Claims
- 1. A process for preparation of R-(-)-3-chloro-1,2-propanediol or R-(-)-3-bromo-1,2-propanediol which comprises cultivating and proliferating, in a medium containing racemate 3-chloro-1,2-propanediol or racemate 1-bromo-1,2-propanediol as an essential carbon source, a bacterium belonging to the genus Pseudomonas which is selected from the group consisting of Pseudomonas sp. DS-K-9D1, Pseudomonas sp. DS-K-2D1 and Pseudomonas sp. DS-K-14A4 which are deposited with Fermentation Research Institute, Agency of Industrial Science and Technology, Japan as Deposit Nos. FERM BP-3095, FERM BP-3096 and FERM BP-3097, respectively, or its culture cell, and recovering R-(-)-3-chloro-1,2-propanediol or R-(-)-3-bromo-1,2-propanediol from the resulting culture broth.
- 2. The process of claim 1 in which each of said bacteria has the ability to grow and proliferate when cultivated in a medium containing racemate 3-chloro-1,2-propanediol or racemate 3-bromo-1,2-propanediol as the sole carbon source.
- 3. The process of claim 1 for preparing R-(-)-3-chloro-1,2-propanediol wherein the medium contains racemate 3-chloro-1,2-propanediol.
- 4. The process of claim 1 for preparing R-(-)-3-chloro-1,2-propanediol wherein the medium contains racemate 3-bromo-1,2-propanediol.
- 5. The process of claim 1 wherein the cultivation is carried out under an aerobic condition.
- 6. The process of claim 1 wherein the cultivation is carried out at a temperature of about 20.degree. C. to about 40.degree. C.
- 7. The process of claim 1 wherein the cultivation is carried out at a pH of about 5 to about 10.
- 8. The process of claim 1 wherein the bacterium is Pseudomonas sp. DS-K-9D1.
- 9. The process of claim 1 wherein the bacterium is Pseudomonas sp. DS-K-2D1.
- 10. The process of claim 1 wherein the bacterium is Pseudomonas sp. DS-K-14A4.
- 11. The process of claim 1 wherein the cultivation is carried out under aerobic conditions at a temperature of from about 25.degree. to 37.degree. C. at a pH of from about 5.5 to 9.5 at a substrate concentration in the reaction mixture of from 0.1 to 10% (v/v) for from about 48 to 80 hours.
- 12. The process of claim 11 wherein the cultivation is carried out in a medium containing racemate 3-chloro-1,2-propanediol as the sole carbon source.
- 13. The process of claim 1 in which the cultivation is carried out in a medium containing racemate 3-bromo-1,2-propanediol as the sole carbon source.
Priority Claims (1)
Number |
Date |
Country |
Kind |
1-330367 |
Dec 1989 |
JPX |
|
Parent Case Info
This application is a continuation of application Ser. No. 07/630,046, filed Dec. 19, 1990, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5017484 |
Nakamura et al. |
May 1991 |
|
Foreign Referenced Citations (5)
Number |
Date |
Country |
0224246 |
Mar 1987 |
EPX |
0207636 |
Jul 1987 |
EPX |
0286059 |
Dec 1988 |
EPX |
0317998 |
May 1989 |
EPX |
3-053886 |
Mar 1991 |
JPX |
Continuations (1)
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Number |
Date |
Country |
Parent |
630046 |
Dec 1990 |
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