Claims
- 1. A method of producing antibiotic A21A which comprises cultivating a A21A-producing strain of Streptomyces filipinensis in a fermentation broth containing assimilable sources of carbohydrates, nitrogen and inorganic salts under aerobic conditions until a substantial amount of A21A is produced in the fermentation broth and recovering said antibiotic.
- 2. The method of claim 1 wherein the Streptomyces filipinensis has NRRL 11,044.
- 3. The method of claim 1 wherein the whole fermentation broth is extracted with a water immiscible polar or hydroxylic solvent.
- 4. The method of claim 3 wherein the solvent is selected from the group consisting of methyl formate, ethyl formate, methyl acetate, ethyl acetate, n-butyl acetate, isobutyl acetate, ethyl proprionate, methyl ethyl ketone, cyclohexanone, chloroform, methylene chloride, carbon tetrachloride, ethylene dichloride, 1-chloro-2,2-dimethylpropane, tetrachloroethylene, bromoform and n-butanol.
- 5. The method of claim 4 wherein the polar or hydroxylic solvent is chloroform, methyl ethyl ketone or ethyl acetate.
- 6. The method of claim 5 wherein the antibiotic A21A is recovered in substantially pure form.
Parent Case Info
This is a division of application Ser. No. 736,794 filed Oct. 29, 1976 now U.S. Pat. No. 4,071,631.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4062948 |
Vos et al. |
Dec 1977 |
|
4065356 |
Maiese et al. |
Dec 1977 |
|
Divisions (1)
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Number |
Date |
Country |
Parent |
736794 |
Oct 1976 |
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