Claims
- 1. A process for producing an immobilized enzyme composition which comprises the steps of
- reacting enzyme and/or enzyme containing microorganic cells with a cross-linking agent and an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.05, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0.degree. C. to about 30.degree. C,
- adjusting the water contents of the resulting reaction products to from about 25 to about 80 wt.%,
- subjecting to extrusion molding the adjusted reaction products with an extruder at a temperature from about 0.degree. C. to 25.degree. C., thereby obtaining an extrudate,
- pelletizing said extrudate, and then
- drying the pellets at a temperature from about 40.degree. C. to 80.degree. C. for about 15 minutes to 20 hours.
- 2. The process of claim 1 wherein the enzyme-containing microorganic cells are selected from the group consisting of cells containing glucose isomerase, glucoamylase and aminoacylase.
- 3. The process of claim 1 wherein said enzyme is selected from the group consisting of glucose isomerase, glucoamylase and aminoacylase.
- 4. The process of claim 1, wherein the extruder is a twin screw horizontal type extruder.
- 5. A process for producing an immobilized enzyme composition which comprises the steps of
- reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0.degree. C. to about 30.degree. C.,
- adjusting the water content of the resulting reaction products to from about 25 to about 80 wt.%,
- subjecting to extrusion molding the adjusted reaction products in the presence of a cross-linking agent with an extruder at a temperature of from about 0.degree. C. to 25.degree. C., thereby obtaining an extrudate,
- pelletizing said extrudate, and then drying the resulted pellets at a temperature from about 40.degree. C. to 80.degree. C. for about 15 minutes to 20 hours.
- 6. A process for producing an immobilized enzyme composition which comprises the steps of
- reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0.degree. C. to about 30.degree. C.,
- adjusting the water content of the resulting reaction products to from about 25 to about 80 wt.%,
- subjecting to extrusion molding said adjusted products with an extruder at a temperature from about 0.degree. C. to 25.degree. C., thereby obtaining an extrudate,
- contacting said extrudate with a solution of a cross-linking agent,
- pelletizing the resulting extrudate, and then drying the pellets at a temperature from about 40.degree. C. to 80.degree. C. for about 15 minutes to 20 hours.
- 7. A process for producing an immobilized enzyme composition which comprises the steps of
- reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0.degree. C. to about 30.degree. C.,
- adjusting the water contents of the resulting reaction products to from about 25 to about 80 wt.%,
- subjecting to extrusion molding the adjusted reaction products with an extruder at a temperature from about 0.degree. C. to 25.degree. C., thereby obtaining an extrudate,
- pellitizing said extrudate, and then drying the pellets at a temperature from about 40.degree. C. to 80.degree. C. for about 15 minutes to 20 hours, and contacting said dried pellets with a solution of a cross-linking agent.
- 8. The process of any of claims 1, 5, 6 or 7 wherein said extruder has a screen of 0.1 to 2.0 mm.
- 9. The process any of claims 1, 5, 6 or 7, wherein the crosslinking agent is dialdehyde starch.
- 10. The process any of claims 1, 5, 6 or 7, wherein the crosslinking agent is glutaraldehyde.
- 11. The process any of claims 1, 5, 6 or 7, wherein the crosslinking agent is selected from the group consisting of condensation products of ureaformaldehyde, succinic anhydride, maleic anhydride copolymer, epichlorohydrin, epoxy resin, cyanuric acid chloride, aminodichlorotriazine, toluene diisocyanate, dichlorobutene and Woodward's reagent.
- 12. The process any of claims 1, 5, 6 or 7, wherein the weight ratio of the enzyme and/or enzyme containing microorganic cells to the anion exchange high molecular substance is in the range of from 1:0.05 to 1:0.2.
- 13. The product of the process any of claims 1, 5, 6 or 7.
Priority Claims (1)
Number |
Date |
Country |
Kind |
51/35471 |
Mar 1976 |
JPX |
|
Parent Case Info
This is a continuation of application Ser. No. 781,921, filed Mar. 28, 1977, now abandoned.
US Referenced Citations (6)
Continuations (1)
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Number |
Date |
Country |
Parent |
781921 |
Mar 1977 |
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