Claims
- 1. A process for producing optically active 1,3 butandiol, which comprises treating 4-hydroxy-2-butanone with a microorganism, capable of asymmetrically reducing 4-hydroxy-2-butanone into either (R)-1,3-butanediol or (S)-1,3-butanediol, said microorganism being selected from the group consisting of:
- Ambrosiozyma cicatricosa
- Bacillus cereus
- Bacillus subtilis
- Candida cariosilignicola
- Candida arborea
- Candida kefyr (Beijerinck)
- Candida krusei (Castellani)
- Candida succiphila
- Candida utilis
- Citrobacter freundii
- Corynebacterium michiganense
- Hansenula polymorpha
- Hansenula minuta
- Hansenula subpelliculosa
- Hansenula fabianii
- Hansenula wickerhamii
- Hansenula wingei
- Issatchenkia scutulata var. scutulata
- Issatchenkia scutulata
- Kluyveromyces drosophilarum
- Kluyveromyces lactis
- Pichia cellobiosa
- Pichia heedii
- Pichia lindneri
- Pichia opuntiae var. thermotolerans
- Pichia pastoris
- Pichia trehalophia
- Protaminobacter ruber
- Pseudomonas diminuta
- Pseudomonas fluorescens
- Pseudomonas putida
- Stephanoascus ciferrii
- Rhodotorula rubra
- Selenotila peltata
- Schizosaccharomyces pombe
- Xanthomonas maltophilia
- Brevibacterium iodinum
- Candida rugosa
- Candida parapsilosis
- Geotrichum candidum
- Geotrichum rectangulatum
- Geotrichum klebahnii
- Geotrichum fermentans
- Geotrichum capitatum
- Geotrichum eriense
- Klebsiella pneumoniae
- Lodderomyces elongisporus
- Saccharomycopsis lipolytica
- Trichosporon cutaneum and
- Trichosporon capitatum
- and (2) collecting the (R)-1,3-butanediol or (S)-1,3-butanediol thus formed.
- 2. A process according to claim 1, wherein said microorganism is capable of reducing 4-hydroxy-2-butanone to (R)-1,3-butanediol and said microorganism is selected from the group consisting of:
- Ambrosiozyma cicatricosa
- Bacillus cereus
- Bacillus subtilis
- Candida cariosilignicola
- Candida arborea
- Candida kefyr (Beijerinck)
- Candida krusei (Castellani)
- Candida succiphila
- Candida utilis
- Citrobacter freundii
- Corynebacterium michiganense
- Hansenula polymorpha
- Hansenula minuta
- Hansenula subpelliculosa
- Hansenula fabianii
- Hansenula wickerhamii
- Hansenula wingei
- Issatchenkia scutulata var. scutulata
- Issatchenkia scutulata
- Kluyveromyces drosophilarum
- Kluyveromyces lactis
- Pichia cellobiosa
- Pichia heedii
- Pichia lindneri
- Pichia opuntiae var. thermotolerans
- Pichia pastoris
- Pichia trehalophia
- Protaminobacter ruber
- Pseudomonas diminuta
- Pseudomonas fluorescens
- Pseudomonas putida
- Stephanoascus ciferrii
- Rhodotorula rubra
- Selenotila peltata
- Schizosaccharomyces pombe and
- Xanthomonas maltophilia.
- 3. A process as according to claim 1, wherein said microorganism is capable of reducing 4-hydroxy-2-butanone to (S)-1,3-butanediol and said microorganism is selected from the group consisting of:
- Brevibacterium iodinum
- Candida rugosa
- Candida parapsilosis
- Geotrichum candidum
- Geotrichum rectangulatum
- Geotrichum klebahnii
- Geotrichum fermentans
- Geotrichum capitatum
- Geotrichum eriense
- Klebsiella pneumoniae
- Lodderomyces elongisporus
- Saccharomycopsis lipolytica
- Trichosporon cutaneum and
- Trichosporon capitatum.
- 4. A process for producing optically active 1,3 butandiol, which comprises treating 4-hydroxy-2-butanone with a microorganism, capable of asymmetrically reducing 4-hydroxy-2-butanone into either (R)-1,3-butanediol or (S)-1,3-butanediol, said microorganism being selected from the group consisting of:
- ______________________________________Ambrosiozyma cicatricosa IFO 1846Bacillus cereus AHU 1355Bacillus subtilis IFO 3007Candida kefyr (Beijerinck) DSM 70073Candida krusei (Castellani) DSM 70075Citrobacter freundii AHU 1534Corynebacterium michiganense IFO 13762Hansenula polymorpha ATCC 26012Hansenula minuta DSM 70274Hansenula subpelliculosa IFO 0808Hansenula fabianii IFO 1254Hansenula wickerhamii DSM 70280Hansenula wingei DSM 70281Issatchenkia scutulata var. IFO 10070scutulataIssatchenkia scutulata IFO 10069Kluyveromyces drosophilarum IFO 1012Pichia cellobiosa DSM 2147Pichia heedii IFO 10019Pichia heedii IFO 10020Pichia pastoris DSM 70382Pichia trehalophia DSM 70391Protaminobacter ruber IAM 1081Pseudomanas diminuta IFO 12697Pseudomanas fluorescens IFO 3081Pseudomonas putida IFO 3738Stephanoascus ciferrii IFO 1854Rhodotorula rubra IFO 0383Selenotila peltata DSM 70579Schizosaccharomyces pombe IFO 0363Xanthomonas maltophilia IFO 12690Brevibacterium iodinum IFO 3558Candida rugosa IFO 1364Candida parapsilosis IFO 0640Candida parapsilosis IFO 0708Geotrichum candidum IFO 4601Geotrichum candidum IFO 5767Geotrichum rectangulatum JCM 1750Geotrichum klebahnii JCM 2171Geotrichum fermentans JCM 2467Geotrichum capitatum JCM 3908Geotrichum eriense JCM 3912Klebsiella pneumoniae IFO 12009Lodderomyces elongisporus IFO 1676Saccharomycopsis lipolytica IFO 1550Trichosporon cutaneum IFO 1198 andTrichosporon capitatum IFO 0743______________________________________
- and (2) collecting the (R)-1,3-butanediol or (S)-1,3-butanediol thus formed.
- 5. A process according to claim 4, wherein said microorganism is capable of reducing 4-hydroxy-2-butanone to (R)-1,3-butanediol and said microorganism is selected from the group consisting of:
- ______________________________________Ambrosiozyma cicatricosa IFO 1846Bacillus cereus AHU 1355Bacillus subtilis IFO 3007Candida cariosilignicola DSM 2148Candida kefyr (Beijerinck) DSM 70073Candida krusei (Castellani) DSM 70075Candida succiphila DSM 2149Candida utilis IFO 0626Citrobacter freundii AHU 1534Corynebacterium michiganense IFO 13762Hansenula polymorpha ATCC 26012Hansenula minuta DSM 70274Hansenula subpelliculosa IFO 0808Hansenula fabianii IFO 1254Hansenula wickerhamii DSM 70280Hansenula wingei DSM 10281Issatchenkia scutulata var. scutulata IFO 10070Issatchenkia scutulata IFO 10069Kluyveromyces drosophilarum IFO 1012Kluyveromyces lactis IFO 1903Pichia cellobiosa DSM 2147Pichia heedii IFO 10019Pichia heedii IFO 10020Pichia pastoris DSM 70382Pichia trehalophia DSM 70391Protaminobacter ruber IAM 1081Pseudomonas diminuta IFO 12697Pseudomonas fluorescens IFO 3081Pseudomonas putida IFO 3738Stephanoascus ciflerrii IFO 1854Rhodotorula rubra IFO 0383Selenotila peltata DSM 70579Schizosaccharomyces pombe IFO 0363 andXanthomonas maltophilia IFO 12690.______________________________________
- 6. A process as according to claim 4, wherein said microorganism is capable of reducing 4-hydroxy-2-butanone to (S)-1,3-butanediol and said microorganism is selected from the group consisting of:
- ______________________________________Brevibacterium iodinum IFO 3558Candida rugosa IFO 1364Candida parapsilosis IFO 0640Candida parapsilosis IFO 0708Geotrichum candidum IFO 4601Geotrichum candidum IFO 5767Geotrichum rectangulatum JCM 1750Geotrichum klebahnii JCM 2171Geotrichum fermentans JCM 2467Geotrichum capitatum JCM 3908Geotrichum eriense JCM 3912Klebsiella pneumoniae IFO 1209Lodderomyces elongisporus IFO 1676Saccharomycopsis lipolytica IFO 1550Trichosporon cutaneum IFO 1198 andTrichosporon capitatum IFO 0743.______________________________________
- 7. A process according to claim 4, wherein said microorganism has been immobilized.
- 8. A process according to claim 4, wherein said reduction is accomplished by an enzyme isolated from said microorganism.
- 9. A process according to claim 4, wherein said microorganism is collected, washed and resuspended in water or a buffer.
- 10. A process according to claim 4, wherein said microorganism is collected, washed, treated with acetone and lyophilized.
- 11. A process according to claim 10, wherein said microorganism is further immobilized.
- 12. A process for producing optically active 1,3-butanediol, which comprises:
- (a) centrifuging, washing and resuspending in a buffer solution cells of microorganisms selected from the group consisting of:
- ______________________________________Candida utilis IFO 0639Candida parapsilosis IFO 1396Geotrichum candidum IFO 5368Kluyveromyces lactis IFO 1267 andPichia opuntiae var. IFO 10025thermotolerans______________________________________
- (b) treating 4-hydroxy-2-butanone with this solution; and
- (c) recovering either (R)-1,3-butanediol or (S)-1,3-butanediol.
- 13. The process according to claim 12, further comprising immobilizing said cells in said buffer solution.
Priority Claims (5)
Number |
Date |
Country |
Kind |
63-105024 |
Apr 1988 |
JPX |
|
63-105025 |
Apr 1988 |
JPX |
|
63-105026 |
Apr 1988 |
JPX |
|
63-225078 |
Sep 1988 |
JPX |
|
63-253019 |
Oct 1988 |
JPX |
|
Parent Case Info
This application is a divisional of application Ser. No. 07/964,264, filed on Oct. 21, 1992, which in turn is a divisional of application Ser. No. 07/449,929, filed on Dec. 7, 1989, and now issued as U.S. Pat. No. 5,219,757.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5219757 |
Matsuyama et al. |
Jun 1993 |
|
Non-Patent Literature Citations (6)
Entry |
Christen M. et al., J. Chem. Soc. Chem. Commun 1988 pp. 264-266. |
Chemical Abstracts, vol. 100, No. 100:21558m, p. 402 (1984). |
Chemical Abstracts, vol. 27, No. 7 (1933). |
Levene et al., J. Biol. Chem., 94, pp. 361-366, (1931). |
Neuberg et al., Biochem. Z., 92, pp. 96-110 (1918). |
Murakami et al., Bulletin of the Chemical Society of Japan, vol. 53, No. 5, pp. 1356-1360, (1980). |
Divisions (2)
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Number |
Date |
Country |
Parent |
964264 |
Oct 1992 |
|
Parent |
449929 |
Dec 1989 |
|