Claims
- 1. A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of a sulfur containing organic compound which induce the formation of the products (FIC) and of a carbon source so as to produce an isolatable amount of the products (FIC) in the nutrient medium; and
- (b) recovering the products (FIC) from the nutrient medium, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
- 2. A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in a composition selected from the group consisting of cysteine, garlic extract, milk, whey, yeast, a yeast extract, molasses and a protein digest which induce the formation of the products (FIC), of a protein source and of a carbon source, so as to produce the products (FIC) in the nutrient medium; and
- (b) treating the growth medium which has been incubated so as to recover isolatable amounts of the products (FIC); and
- (c) recovering the products FIC from the nutrient medium, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
- 3. The process of claim 2 wherein the nutrient medium which is incubated contains the yeast extract for the growth factors, non-fat dry milk as the protein source; lactose, sucrose, dextrose, fructose or molasses as the carbon source and corn steep liquor as a source of minerals.
- 4. The process of claim 3 wherein the nutrient medium contains a compound selected from the group consisting of cinnamic acid, an alkali metal propionate and phenylalanine, in an amount which increases the production of the products (FIC).
- 5. The process of claim 2 wherein in step (b) the growth medium with the live cells is dried.
- 6. A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in a composition selected from the group consisting of cysteine, garlic extract, milk, whey, yeast, a yeast extract, molasses and a protein digest which induce the formation of the products (FIC), a protein source and of a carbon source to thereby produce isolatable amounts of products (FIC) in the nutrient medium; and
- (b) treating the nutrient medium which has been incubated to recover the products (FIC) with removal of water from the nutrient medium to produce the products (FIC), wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
- 7. The process of claim 6 wherein in step (b) the nutrient medium is lyophilized.
- 8. The process of claim 6 wherein the Lactobacillus are incubated in the nutrient medium at a temperature of between about 100 and 50.degree. C.
- 9. The process of claim 6 wherein the nutrient medium contains the yeast extract, whey or non-fat dry milk as the protein source, lactose, sucrose, dextrose, fructose or molasses as the carbon source and corn steep liquor as a source of minerals.
- 10. The process of claim 9 wherein the nutrient medium contains in addition a manganese salt, a magnesium salt and an alkali metal phosphate.
- 11. A process for producing yeast and mold growth inhibiting products (FIC) which comprises:
- (a) incubating cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in compositions selected from the group consisting of cysteine, garlic extract, milk, whey, yeast, a yeast extract, molasses and a protein digest which induce the formation of the products (FIC) and of a carbon source so as to produce the products (FIC) in the nutrient medium;
- (b) mixing the incubated nutrient medium with an organic solvent which extracts the products (FIC) into the solvent;
- (c) separating the solvent with the extracted products (FIC) from the nutrient medium; and
- (d) separating the products (FIC) from the solvent, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
- 12. The process of claim 11 wherein the solvent is n-butanol.
- 13. The process of claim 11 wherein the solvent is acetone.
- 14. The process of claim 11 wherein the solvent is ethanol.
- 15. The process of claim 11 wherein the solvent mixture is cooled to thereby precipitate impurities.
- 16. The process of claim 11 wherein in addition the separated products are purified to separate impurities using liquid chromatography.
- 17. The process of claim 11 wherein in addition the products (FIC) are purified by liquid dialysis which passes compounds through a membrane having a molecular size of about 1000 or less and wherein the products pass through the membrane and are collected.
- 18. The process of claim 17 wherein in addition the purified products (FIC) are further purified by liquid chromatography using a solvent mixture to produce separation of the products (FIC) from impurities.
- 19. The process of claim 18 wherein in addition the products (FIC) with out the impurities are subjected to a further purification using liquid chromatography.
- 20. The process of claim 11 wherein the nutrient medium contains the yeast extract and the protein digest.
- 21. The process of claim 1 wherein the nutrient medium which is to be incubated is pasteurized or sterilized prior to incubating the cells.
- 22. A process for producing fungal growth inhibiting products (FIC) which comprises:
- (a) incubating cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium for the cells containing effective amounts of growth factors present in cysteine, garlic extract, milk, whey, yeast, yeast extract, molasses or protein digest which induce the formation of the products (FIC), of a protein source and of a carbon source so as to produce the products (FIC) in the nutrient medium; and
- (b) recovering the products (FIC) from the nutrient medium and cells, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
- 23. A process for producing fungal growth inhibiting products (FIC) which comprises:
- (a) incubating live cells of a Lactobacillus having all of the identifying characteristics of Lactobacillus casei var. rhamnosus NRRL-B-15972 in a nutrient medium containing effective amounts of growth factors for the cells which stimulate the cells to produce an isolatable amount of the products (FIC) in the nutrient medium; and
- (b) recovering the products (FIC) from the nutrient medium, wherein the products (FIC) are polar, non-protein, non-lipid, have a molecular size of less than 1000 daltons, are insoluble in chloroform or hexane and soluble in ethanol, in butanol and acetone, are temperature stable at between -70.degree. C. and 100.degree. C. and are stable at a pH between 3 to 10.
CROSS REFERENCE TO RELATED APPLICATION
This is a continuation of application Ser. No. 07/468,575 filed on Jan. 23, 1990, now abandoned, which is a continuation of Ser. No. 07/082,118, filed Aug. 6, 1987, now abandoned, which is a continuation-in-part of Ser. No. 06/794,468, filed Nov. 4, 1985, now U.S. Pat. No. 4,956,177.
US Referenced Citations (2)
Foreign Referenced Citations (2)
Number |
Date |
Country |
8001045 |
Aug 1980 |
NLX |
1109436 |
Aug 1984 |
SUX |
Non-Patent Literature Citations (6)
Entry |
ATCC catalogue of Bacteria, pp. 115-116, 1989. |
Condensed Chemical Dictionary, p. 502, 1974. |
The Merck Index, p. 768, 1983. |
Vincent et al, J. Bacteriol., vol. 78, pp. 477-484, 1959. |
ATCC Catalogue of Bacteria, 1989, pp. 116-118. |
Rehm et al, "Biotechnology", vol. 5, p. 73, 1983. |
Continuations (2)
|
Number |
Date |
Country |
Parent |
468575 |
Jan 1990 |
|
Parent |
082118 |
Aug 1987 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
794468 |
Nov 1985 |
|