Process for stereoselective synthesis of prostacyclin derivatives

Description

FIELD OF THE INVENTION

The present application relates to a process for producing prostacyclin derivatives and novel intermediate compounds useful in the process.

BACKGROUND OF THE INVENTION

Prostacyclin derivatives are useful pharmaceutical compounds possessing activities such as platelet aggregation inhibition, gastric secretion reduction, lesion inhibition, and bronchodilation.

For convenience, the novel prostacyclin derivatives will be referred to by the trivial, art-recognized system of nomenclature described by N. A. Nelson, J. Med. Chem. 17:911 (1974) for prostaglandins. Accordingly, all of the novel prostacyclin derivatives herein will be named as 9-deoxy-PGF

1

-type compounds.

The prostacyclin derivatives prepared by the method disclosed in the '075 patent are as follows:

wherein L

1

is α-R

3

:β-R

4

, α-R

4

: β-R

3

, or a mixture of α-R

3

:β-R

4

and α-R

4

: β-R

3

, wherein R

3

and R

4

are hydrogen, methyl, or fluoro, being the same or different, with the proviso that one of R

3

and R

4

is fluoro only when the other is hydrogen or fluoro;

wherein M

1

is α-OH: β-R

5

or α-R

5

: β-OH, wherein R

5

is hydrogen or methyl;

wherein R

7

is

(1) -C

m

H

2m

-CH

3

, wherein m is an integer from one to 5, inclusive,

(2) phenoxy optionally substituted by one, two or three chloro, fluoro, trifluoromethyl, (C

1

-C

3

)alkyl, or (C

1

-C

3

)alkoxy, with the proviso that not more than two substituents are other than alkyl, with the proviso that R

7

is phenoxy or substituted phenoxy, only when R

3

and R

4

are hydrogen or methyl, being the same or different,

(3) phenyl, benzyl, phenylethyl, or phenylpropyl optionally substituted on the aromatic ring by one, two or three chloro, fluoro, trifluoromethyl, (C

1

-C

3

)alkyl, or (C

1

-C

3

)alkoxy, with the proviso that not more than two substituents are other than alkyl,

(4) cis—CH=CH-CH

2

-CH

3

,

(5) (CH

2

)

2

-CH(OH)-CH

3

, or

(6) -(CH

2

)

3

—CH=C(CH

3

)

2

;

wherein -C(L

2

)-R

7

taken together is

(1) (C

4

-C

7

)cycloalkyl optionally substituted by one to 3 (C

1

-C

5

) alkyl;

(2) 2-(2-furyl)ethyl,

(3) 2-(3thienyl)ethoxy, or

(4) 3-thienyloxymethyl;

wherein R

8

is hydroxy, hydroxymethyl, or hydrogen;

wherein

(1) R

20

, R

21

, R

22

, R

23

, and R

24

are all hydrogen with R

22

being either α-hydrogen or β-hydrogen,

(2) R

20

is hydrogen, R

21

and R

22

taken together form a second valence bond between C-9 and C-6

a

, and R

23

taken together form a second valence bond between C-8 and C-9 or are both kydrogen, or

(3) R

22

, R

23

, and R

24

are all kydrogen, with R

22

being either α-hydrogen or β-hydrogen, and

(a) R

20

and R

21

taken together are oxo, or

(b) R

20

is kydrogen and R

21

is hydroxy, being α-hydroxy or β-hydroxy;

wherein X

1

is

(1) -COOR

1

, wherein R

1

is

(a) hydrogen,

(b) (C

1

-C

12

)alkyl,

(c) (C

3

-C

10

)cycloalkyl,

(d) (C

6

-C

12

)aralkyl,

(e) phenyl, optionally substituted with one, 2 or 3 chloro or (C

1

-C

1

)alkyl,

(f) phenyl substituted in the para position by

(i) -NH-CO-R

25

,

(ii) -CO-R

26

,

(iii) -O-CO-R

54

, or

(iv) -CH=N-NH-CO-NH

2

wherein R

25

is methyl, phenyl, acetamidophenyl, benzamidophenyl, or -NH

2

: R

26

is methyl, phenyl, -NH

2

, or methoxy; and R

54

is phenyl or acetamidophenyl; inclusive, or

(g) a pharmacologically acceptiable cation;

(2) -CH

2

OH,

(3) -COL

4

, wherein L

4

is

(a) amino of the formula - - NR

51

R

52

, wherein R

51

and R

52

are

(i) hydrogen,

(ii) (C

1

- C

12

)alkyl,

(iii) (C

3

-C

10

)cycloalkyl,

(iv) (C

7

- C

12

)aralkyl,

(v) phenyl, optionally substituted with one, 2 or 3 chloro, (C

1

-C

3

)alkyl, hydroxy, carboxy, (C

2

-C

5

)alkoxycarbonyl, or nitro,

(vi) (C

2

-C

5

)carboxyalkyl,

(vii) (C

2

-C

5

)carbamoylalkyl,

(viii) (C

2

-C

5

)cyanoalkyl,

(ix) (C

3

-C

6

)acetylalkyl,

(x) (C

7

-C

11

)benzoalkyl, optionally substituted by oe, 2 or 3 chloro, (C

1

-C

3

)alkyl, hydroxy, (C

1

-C

3

)alkoxy, carboxy, (C

2

-C

5

)alkoxycarbonyl, or nitro,

(xi) pyridyl, optionally substituted by one, 2 or 3 chloro, (C

1

-C

3

)alkyl, or (C

1

-C

3

)alkoxy,

(xii) (C

6

-C

9

)pyridylalkyl optionally substituted by one, 2 or 3 chloro, (C

1

-C

3

)alkyl, hydroxy, or (C

1

-C

3

)alkyl,

(xiii) (C

1

-C

4

)kydroxyalkyl,

(xiv) (C

1

-C

4

)dihydroxyalkyl,

(xv) (C

1

-C

4

)trihydroxyalkyl,

with the further proviso that not more than one of R

51

and R

52

is other than hydrogen or alkyl,

(b) cycloamino selected from the group consisting of lyrolidino, piperidino, morpholino, piperazino, hexamethyleneimino, pyrrolino, or 3,4-didehydropiperidinyl optionally substituted by one or 2 (C

1

-C

12

)alkyl of one to 12 carbon atoms, inclusive,

(c) carbonylamino of the formula -NR

53

COR

51

, wherein R

53

is hydrogen or (C

1

-C

4

)alkyl and R

51

is other than hydrogen, but otherwise as defined above,

(d) sulfonylamino of the formula -NR

53

SO

2

R

51

, wherein R

51

and R

53

are as defined in (c),

(4) -CH

2

NL

2

L

3

, wherein L

2

and L

3

are kydrogen or (C

1

-C

4

)alkyl, being the same or different, or the pharmacologically acceptable acid addition salts thereof when X

1

is -CH

2

NL

2

L

3

,

wherein Y

1

is trans-CH=CH-, cis-CH=CH-, CH

2

CH

2

-, or -C≡C-; and

wherein Z

4

is -CH

2

- or -(CH

2

)-CF

2

, wherein ƒ is zero, one, 2 or 3.

When X

1

is -COOR

1

of the Formulac in the '075 patent, the novel compounds so described are used for the purposes described and are in free acid form, in ester form, or in pharmacologically acceptable salt form. When the ester form is used, the ester is any of those within the above definition of R

1

. However, it is preferred that the ester be alkyl of one to 12 carbon atoms, inclusive. Of the alkyl esters, methyl and ethyl are especially preferred for optimum absorption of the compound by the body or experimental animal system; and straight-chain oxtyl, nonyl, decyl, undecyl, and dodecyl are especially preferred for prolonged activity.

Pharmacologically acceptable salts of the novel prostagladin analogs of this invention for the purposes described are those with pharmacologically acceptiable metal cations, ammonia, amine cations, or quaternary ammonium cations.

Especially preferred metal cations are those derived from the alkali metals, e.g., lithium, sodium, and potassium, and from the alkaline earth metals, e.g., magnesium and calcium, although cationic forms of other metals, e.g., aluminum, zinc, and iron are within the scope of this invention.

Pharmacologically acceptable amine cations are those derived from primary, secondary, and tertiary amines. Example of suitable amines are methylamine, dimethylamine, trimethylamine, ethylamine, dibutylamine, triisopropylamine, N-methylhexylamine, decylamine, dodecylamine, allylamine, crotylamine, cyclopentylamine, dicyclohexylamine, benzylamine, dibenzylamine, α-phenylethylamine, β-phenylethylamine, ethylenediamine, diethylenetriamine, adamantylamine, and the like aliphatic, cycloaliphatic, araliphatic amines containing up to and including about 18 carbon atoms, as well as heterocyclic amines, e.g., piperidine, morpholine, pyrrolidine, piperazie, and lower-alkyl derivatives thereto, e.g., 1-methylpiperidine, 4-ethylmorpholine, 1-isopropylpyrrolidine, 2-methylpyrrolidine, 1,4-dimethylpiperazine, 2-methylpiperidine, and the like as well as amines containing water-solubilizing or hydrophilic groups, e.g., mono-, di-, and triethanolamine, ethyldiethanolamine, N-butylethanolamine, 2-amino-1-butanol, 2-amino-2-ethyl,-1,3-propanediol, 2-amino-2-methyl-1-propanol, tris(hydroxymethyl) aminomethane, N-phenylethanolamine, N-(p-tert-amylphenyl)-diethanolamine, galactamine, N-methylglycamine, N-methylglucosamine, ephedrine, phenylephrine, epinephrie, procaine, and the like. Further useful amine salts of the basic amino acid salt, e.g., lysie and arginine.

Examples of suitable pharmacologically acceptable quaternary ammonium cations are tetramethylammonium, tetraethylammonium, benzyltrimethylammonium, phenyltriethylammonium, and the like.

U.S. Pat. No. 4,306,075 discloses methods for making prostacyclin derivatives. However, these and other known processes involve a large number of steps. It is an object of the present invention to provide an improved method of preparing prostacyclin derivatives involving fewer steps.

SUMMARY OF THE INVENTION

The present invention relates to a process for preparing 9-deoxy-PGF

1

-type compounds by a process that is stereoselective and requires fewer steps than the prior art. The invention also relates to novel intermediates prepared during the synthesis of the 9-deoxy-PGF

1

-type compounds.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

In one embodiment, the present invention relates to an improved stereoselective method for making 9-deoxy-PGF

1

-type compounds comprising converting a compound of the formula:

into a compound of the following formula:

wherein Z is O, S, CH

2

, or NR

8

in which R

8

is H, alkyl or aryl;

X is H, CN, OR

9

, or COOR

9

in which R

9

is alkyl, THP or TBDMS;

wherein n is 0, 1, 2, or 3;

wherein Y

1

is trans-CH═CH—, cis-CH═CH—, —CH

2

(CH

2

)

m

—, or —C≡C—; m is 1,2, or 3;

wherein R

1

is an alcohol protecting group;

wherein R

7

is

(1) —C

p

H

2p

—CH

3

, wherein p is an integer from one to 5, inclusive,

(2) phenoxy optionally substituted by one, two or three chloro, fluoro, trifluoromethyl, (C

1

-C

3

)alkyl, or (C

1

-C

3

)alkoxy, with the proviso that not more than two substituents are other than alkyl, with the proviso that R

7

is phenoxy or substituted phenoxy, only when R

3

and R

4

are hydrogen or methyl, being the same or different,

(3) phenyl, benzyl, phenylethyl, or phenylpropyl optionally substituted on the aromatic ring by one, two or three chloro, fluoro, trifluoromethyl, (C

1

-C

3

)alkyl, or (C

1

-C

3

)alkoxy, with the proviso that not more than two substituents are other than alkyl,

(4) cis-CH═CH—CH

2

—CH

3

,

(5) —(CH

2

)

2

—CH(OH)—CH

3

, or

(6) —(CH

2

)

3

—CH═C(CH

3

)

2

;

wherein —C(L

1

)—R

7

taken together is

(1) (C

4

-C

7

)cycloalkyl optionally substituted by one to 3 (C

1

-C

5

) alkyl;

(2) 2-(2-furyl)ethyl,

(3) 2-(3-thienyl)ethoxy, or

(4) 3-thienyloxymethyl;

wherein M

1

is α-OH:β-R

5

or α-R

5

:β-OH, wherein R

5

is hydrogen or methyl; and

wherein L

1

is α-R

3

:β-R

4

, α-R

4

:β-R

3

, or a mixture of α-R

3

:β-R

4

and α-R

4

:β-R

3

,

wherein R

3

and R

4

are hydrogen, methyl, or fluoro, being the same or different, with the proviso that one of R

3

and R

4

is fluoro only when the other is hydrogen or fluoro.

Preferably, the above conversion is carried out through cobalt-mediated cyclization, in which a complex is formed with the alkynyl group of the starting compound, which decomposes upon heating to form a tricyclic structure. More preferably, this cyclization is carried out by reacting Co

2

(CO)

8

with the above compound of the formula:

using a suitable non-reactive solvent. Preferably, the non-reactive solvent is a chlorinated solvent, a hydrocarbon solvent, or an aromatic solvent. More preferably, the non-reactive solvent is CH

2

Cl

2

, toluene, isooctane, and heptane.

In the case of carrying out the cobalt-mediated cyclization with CH

2

Cl

2

, after reacting Co

2

(CO)

8

with the above compound of the formula:

in the presence of CH

2

Cl

2

to form a complex with the alkynyl group, preferably the CH

2

Cl

2

is removed in a subsequent step and replaced with CH

3

CN followed by heating in an inert gas atmosphere, such as argon, nitrogen, or carbon monoxide, which decomposes the complex to form the above tricyclic compound.

Although Co

2

(CO)

8

contributes a carbonyl during the reaction, it is not necessary to react equal amounts of the starting compound of the above formula and Co

2

(CO)

8

. It is also possible to use the Co

2

(CO)

8

in a catalytic way, by introducing a relatively small amount of Co

2

(CO)

8

and also introducing CO into the reaction mixture (e.g., by bubbling CO into the reaction mixture) in the presence of light which catalyzes the transfer of CO through a Co-mediated complex formed with the above compound of the formula:

In another preferred embodiment, the present invention relates to an improved stereoselective method for making 9-deoxy-PGF

1

-type compounds comprising the following reaction:

wherein n is 0, 1, 2, or 3;

wherein Y

1

is trans-CH═CH—, cis-CH═CH—, —CH

2

(CH

2

)

m

—, or —C═C—; m is 1,2, or 3;

wherein R

1

is an alcohol protecting group;

wherein R

7

is

(1) —C

p

H

2p

—CH

3

, wherein p is an integer from one to 5, inclusive,

(2) phenoxy optionally substituted by one, two or three chloro, fluoro, trifluoromethyl, (C

1

-C

3

)alkyl, or (C

1

-C

3

)alkoxy, with the proviso that not more than two substituents are other than alkyl, with the proviso that R

7

is phenoxy or substituted phenoxy, only when R

3

and R

4

are hydrogen or methyl, being the same or different,

(3) phenyl, benzyl, phenylethyl, or phenylpropyl optionally substituted on the aromatic ring by one, two or three chloro, fluoro, trifluoromethyl, (C

1

-C

3

)allyl, or (C

1

-C

3

)alkoxy, with the proviso that not more than two substituents are other than alkyl,

(4) cis-CH═CH—CH

2

—CH

3

,

(5) —(CH

2

)

2

—CH(OH)—CH

3

, or

(6) —(CH

2

)

3

—CH═C(CH

3

)

2

;

wherein —C(L

1

)—R

7

taken together is

(1) (C

4

-C

7

)cycloaklyl optionally substituted by one to 3 (C

1

-C

5

) alkyl;

(2) 2-(2-furyl)ethyl,

(3) 2-(3-thienyl)ethoxy, or

(4) 3-thienyloxymethyl;

wherein M

1

is α-OH:β-R

5

or α-R

5

:β-OH, wherein R

5

is hydrogen or methyl;

wherein L

1

is α-R

3

:β-R

4

, α-R

4

:β-R

3

, or a mixture of α-R

3

:β-R

4

and α-R

4

:β-R

3

,

wherein R

3

and R

4

are hydrogen, methyl, or fluoro, being the same or different, with the proviso that one of R

3

and R

4

is fluoro only when the other is hydrogen or fluoro.

The present invention also relates to a method of making the following compounds utilizing the foregoing reaction:

wherein R

1

is in each case an independently selected alcohol protecting group. Preferred alcohol protecting groups are tertiary butyl dimethyl sily (TBDMS) and tetra hydro pyranyl (THP).

The present invention also relates to the following novel intermediate compounds:

wherein X, Z, Y

1

, M

1

, L

1

, R

1

and R

7

are as defined above.

The present invention is further illustrated by, though in no way limited to, the following examples.

EXAMPLE 1

9-Deoxy-2′,9α-methano-3-oxa4,5,6-trinor-3,7-(1′,3′-inter-phenylene)-13,14-dihydro-PGF

1

Procedure

To a solution of imidazole (29.6 g, 434 mmol, 2.8 eq.) in 1.0 L of methylene chloride were added 25 g (181 mmol) of 3-methoxybenzyl alcohol (1) in 200 ml of methylene chloride. After all material was dissolved, 32.7 g (217 mmol, 1.2 eq.) of t-butyldimethylsilyl chloride was added in portions. The reaction was stirred overnight at room temperature. The mixture was filtered and washed with water and then brine. The organic layer was separated, dried over MgSO

4

, filtered, and evaporated to afford 53 g of a clear yellow oil that was used in the next step without further purification.

Procedure

To a solution of 95 g (376 mmol) of 2 dissolved in 400 ml of hexane under Ar at room temperature were added dropwise 26.5 g (414 mmol, 1.1 eq.) of BuLi in 166 ml of hexane. The mixture was stirred for 2 hours at room temperature, and then the reaction was cooled in an ice bath and 54.6 g (452 mmol) of allyl bromide were added dropwise. The reaction was allowed to warm to room temperature overnight. After stirring for 24 hours, TLC indicated 60% conversion, and the reaction was quenched with saturated NH

4

Cl. The organic layer was separated and washed with Brine, dried over MgSO

4

, and filtered. Evaporation of the solvent yielded a yellow oil which was used in the next reaction without further purification.

Procedure

To a solution 3 (110 g, 376 mmol) in 2.0 L of THF were added 128 g (489 mmol, 1.1eq.) of tetrabutyl ammonium fluoride (TBAF) in 489 ml of THF. The reaction was stirred at room temperature and was complete after 4 hours. The reaction was quenched by adding 500 ml of water. The organic layer was separated and washed with brine and dried over MgSO

4

. Filtration and evaporation of the solvent produced an orange oil which was purified by flash column chromatography, on silica gel using 10-30% ethyl acetate in hexanes as the eluent. The fractions containing the desired product were evaporated to afford 24 g (36% from 3-methoxybenzyl alcohol) of a yellow oil.

Procedures

To a solution of 20.6 g (162 mmol, 1.2 eq.) of oxalyl chloride in 250 ml of CH

2

Cl

2

under Ar at −78° C. were added dropwise 24.2 g (310 mmol) of DMSO in 100 ml of CH

2

Cl

2

. After 10 minutes, 24 g (135 mmol) of 4 in 100 ml of CH

2

Cl

2

were added dropwise. The mixture was stirred at −78° C. for 30 min., and then 68.3 g (675 mmol, 5.0 eq.) of Et

3

N were added. Stirring continued as the reaction warmed to room temperature. The reaction was quenched with H

2

O, washed with saturated NH

4

Cl solution and Brine. The organic layer was separated and dried over MgSO

4

. Filtration and evaporation of the solvent produced a brown oil which was purified by flash column chromatography, on silica gel using 5% ethyl acetate in hexanes as the eluent. The fractions containing the desired compound were evaporated to afford 20.5 g (86%) of a brown oil.

Procedure

Compound A may be synthesized according to S. Takano et al., Chemistry Lett., 1987, p. 2017. To a solution of side chain (A) (1.6 g, 6.72 mmol) in dry THF (10 ml) which was heated to gentle refluxing under argon was added EtMgBr (2.24 ml, 6.72 mmol, 3M solution). After the addition was complete, the resultant solution was refluxed for 20 mil.

The solution was cooled to 0° C. (under argon) and a solution of 5 (1.183 g, 6.72 mmol) in THF (10 ml, dried over molecular sieves) was added dropwise with stirring. After the complete addition, the reaction mixture was allowed to warm to room temperature and stirred for 2-3 hrs. The reaction mixture was cooled to 0° C., diluted with saturated NH

4

Cl solution, concentrated, extracted with ethyl acetate (4×25 ml), dried (MgSO

4

) and the solvent distilled off in vacuo. The crude product (2.65 g) was purified by flash chromatography using 10-30% ether in hexane on silica gel to obtain a colorless oil 1.45 g (52%) of 6.

Procedure

To a solution of alcohol 6 (1.27 g, 13.07 mmol) in dry CH

2

Cl

2

(20 ml) was added pyridinium chlorochromate (PCC) (1.32 g, 6.12 mmol) and the mixture was stirred at room temperature. PCC slowly dissolved and the color of solution turned orange-black after approx. 5 min. Stirring was continued for 3 hrs. The reaction mixture was diluted with ether (100 ml) and filtered through a plug of silica gel. The solid was washed 3 times with ether (3×50 ml). After the solvent was removed, the crude product (1.3 g) was purified by flash chromatography using 10% ether in hexane on silica gel to give 900 mg light yellow oil (71%).

Procedure

STEP I: Preparation of Reagent:

Compound B may be synthesized according to D. S. Mathre et al., J. Org. Chem. 1991, Vol. 56, p. 751; P. Beak, Org. Synth., 1997, p. 23. Compound B (1.08 g, 4.26 mmol) was dissolved in 30 ml of anh. toluene under argon. Trimethylboroxine (C) (0.357 g, 2.84 mmol) was added dropwise and the resulting solution was stirred at room temperature. White solid separated out after 3-4 min. After stirring for 30 min., toluene was distilled out at atmospheric pressure. Again 20 ml of dry toluene were added and distilled out. This distillation was repeated for 2 more times. The solution of reagent in toluene was allowed to cool under argon.

STEP II: Reduction:

A solution of ketone 7 (0.88 g, 2.14 mmol) in dry THF (20 ml) was dried over molecular sieves for 2 hrs and added to the above reagent solution. The resulting solution was cooled to −30° C. (CH

3

CN, CO

2

) under argon and borane-methylsulfide complex (1.07 ml, 10.71 mmol) was added dropwise with stirring. After stirring at −30° C. for 1 hr, the reaction was quenched with methanol (10 ml), diluted with ether (100 ml), washed successively with saturated NH

4

Cl, NaHCO

3

solution and brine, dried (MgSO

4

) and concentrated in vacuo to yield a crude product (2.3 g). The crude product was purified by flash chromatography using 10% ether in hexanes on silica gel to give 770 mg of 8 as a colorless oil (87%).

Procedure

TBDMSCl (0.337 g, 2.23 mmol) and imidazole (0.335 g, 4.65 mmol) were added to the solution of 8 (0.770 g, 1.86 mmol) in DMF (20 ml) at room temperature under argon, and the mixture was stirred at room temperature for 3-4 hrs. After the reaction was quenched with sat. NH

4

Cl, the reaction mixture was extracted with ether (3×50 ml). The combined ether extracts were dried (MgSO

4

) and concentrated in vacuo. The crude oil was purified by chromatography using 5% ether in hexane on silica gel to yield 860 mg of 9 as a colorless oil (88%).

Procedure

STEP I: Complex formation:

Compound 9 (0.840 g, 1.59 mmol) was dissolved in dry CH

2

Cl

2

(15 ml) under argon, and CO

2

(CO)

8

(0.653 g, 1.91 mmol) was added to it and stirred at room temperature under argon. carbon monoxide evolved out slowly, and the solution turned dark brown after 5 min. Stirring was continued for 30 min. at room temperature.

STEP II: Pauson Khand Cyclization

CH

2

Cl

2

was distilled out from the above solution. The complex was dissolved in dry CH

3

CN (50 ml), and the solution was refluxed under argon for 2 hrs. This solvent was distilled out, the crude mass was dissolved in ether and passed quickly through a short column of neutral alumina to yield 850 mg of light brown oil (96%).

Procedure

Compound 10 (0.850 g, 1.53 mmol) was dissolved in absolute ethanol (50 ml). Anh. K

2

CO

3

(0.020 g) and Pd/C (0.550 g, 10%, wet) were added and the mixture was hydrogenated at 20 psi pressure for 13 hrs. The reaction mixture was filtered through celite and concentrated in vacuo. The crude product (800 mg) was purified by chromatography using 10-30% ether in hexane on silica gel to yield 440 mg of colorless oil (67%).

Procedure

A solution of ketone 11 (0.430 g) in 95% ethanol was cooled to −10° C. 10% NaOH (6 ml) and NaBH

4

(0.080 g) were added and the mixture was stirred at −10° C. for 1 hr. Then one more eq. of NaBH

4

(0.080 g) was added and stirring was continued for another 5 hrs. at −10° C. After quenching carefully with glacial acetic acid, the solvent was removed under reduced pressure. Resulting oil was dissolved in ethyl acetate, washed with aq. NaHCO

3

, brine, dried (MgSO

4

) and concentrated in vacuo to obtain 430 mg of colorless oil (98%) which has a single spot on TLC. Further purification was not required.

Procedure

To 400 mg (0.93 mmol) of compound 12 dissolved in methanol (10 ml) was added p-TsOH (20 mg), and the solution was stirred at room temperature until TLC showed completion of the reaction (2 hrs). The solvent was removed in vacuo, the residue was dissolved in CH

2

Cl

2

, washed with sat. NaHCO

3

, dried(MgSO4), and concentrated in vacuo. The crude product was purified by silica gel column chromatography (30% ether in hexanes as eluent) to give 250 mg 13 (78%).

Procedure

n-BuLi (1.1 ml, 1.72 mmol)(1.6 M in hexanes) was added dropwise to a cold (−20° C.) and stirred solution of diphenylphosphine (0.28 g, 1.5 mmol) in anhydrous THF (8 ml) under argon. The reaction mire was warmed to room temperature (20° C.). A solution of diol (13) (0.17 g, 0.49 mmol) in dry THF (0.6 ml) was added dropwise to the reaction mixture and the whole solution was heated to reflux for 3 hrs (TLC shows starting material), heating was stopped and the reaction mixture was cooled again to −20° C. and diphenylphosphine (0.37 g, 1.96 mmol) was added followed by dropwise addition of n-BuLi (1.5 ml, 2.38 mmol)(1.6M in hexanes) under argon. After complete addition, the reaction mixture was warmed to 20° C. and then refluxed for 18 hrs. TLC shows 80-90% conversion (14). The reaction mixture was cooled to −5° C. and then an aqueous solution of NaCl containing 5% conc. HCl was added dropwise to quench the reaction. The reaction mixture was extracted with ethyl acetate 3×20 ml and the combined organic layers were washed with brine and dried (Na

2

SO

4

), filtered and concentrated. The crude product was purified by silica gel column chromatography (50% EtOAc/Hex. as eluent) to give 0.12 g of product (75%) (22 mg of starting diol was recovered).

Procedure

A suspension of compound (14) (0.12 g. 0.37 mmol), chloroacetonitrile (0.56 g, 7.4 mmol) and K

2

CO

3

(0.51 g, 3.7 mmol) in dry acetone (15 ml) was refluxed under Ar for 20 hrs. The reaction mixture was cooled to room temperature and celite (0.5 g) was added. After the mixture was filtered, the solvent was removed under reduced pressure. The crude product was purified by silica gel column chromatography using 1:1 EtOAc/hexanes as eluent to yield 0.12 g of product (95%).

Procedure

Aqueous KOH (0.4 g, 7.12 mmol, water 1.2 ml, 35% solution) was added dropwise to a stirred solution of nitrile compound (15) (0.072 g, 0.21 mmol) in methanol (4 ml) and the reaction mixture was refluxed for 3 hrs. The reaction mixture was cooled to 10° C., dilute aqueous HCl was added to pH 8 and the solvent was removed in vacuo. Ethyl acetate (20 ml) and aqueous NaCl solution (10 ml) were added and the pH of the reaction mixture was acidified to between 2 and 3 by addition of 2% HCl. The reaction mixture was extracted with ethyl acetate (2×20 ml). The combined ethyl acetate extracts were washed with brine, dried (Na

2

SO

4

) and concentrated under reduced pressure. The crude product was purified by silica gel column chromatography using a dichloromethane solution containing 3% methanol and 0.1% acetic acid as eluent to yield 0.076 g of product (95%).

It will be apparent to those skilled in the art that various modifications and variations can be made to the processes and novel intermediates of this invention. Thus, it is intended that the present invention cover such modifications and variations, provided they come within the scope of the appended claims and their equivalents.

The disclosure of all publications cited above are expressly incorporated herein by reference in their entireties to the same extent as if each were incorporated by reference individually.

Claims

1. A stereoselectively produced isomeric compound according to the following formula: that is produced by a process for making 9-deoxy-PGF1-type compounds, the process comprising cyclizing a starting compound of the formula: into a compound of the following formula: by intramolecular cyclization of the enyne, wherein Z is O, S, CH2, or NR8 in which R8 is H, alkyl or aryl; X is H, CN, OR9, or COOR9 in which R9 is H, alkyl, a pharmacologically acceptable cation, THP or TBDMS; wherein n is 0, 1, 2, or 3; wherein Y1 is trans-CH═CH—, cis-CH═CH—, CH2(CH2)m-, or —C≡C—; m is 1,2, or 3; wherein R1 is an alcohol protecting group; wherein R7 is (1) —C2H2p—CH3, wherein p is an integer from one to 5, inclusive, (2) phenoxy optionally substituted by one, two or three chloro, fluoro, trifluoromethyl, (C1-C3)alkyl, or (C1-C3)alkoxy, with the proviso that not more than two substituents are other than alkyl, with the proviso that R7 is phenoxy or substituted phenoxy, only when R3 and R4 are hydrogen or methyl, being the same or different, (3) phenyl, benzyl, phenylethyl, or phenylpropyl optionally substituted on the aromatic ring by one, two or three chloro, fluoro, trifluoromethyl, (C1-C3)alkyl, or (C1-C3)alkoxy, with the proviso that not more than two substituents are other than alkyl, (4) cis-CH═CH—CH2—CH3, (5) —(CH2)2—CH(OH)—CH3, or (6) —(CH2)3—CH═C(CH3)2; wherein —C(L1)—R7 taken together is (1) (C4-C7)cycloalkyl optionally substituted by one to 3 (C1-C5) alkyl; (2) 2-(2-furyl)ethyl, (3) 2-(3-thienyl)ethoxy, or (4) 3-thienyloxymethyl; wherein M1 is α-OH:β-R5 or α-R5:β-OH or α-OR1:β-R5 or α-R5:β-OR1, wherein R5 is hydrogen or methyl and R1 is an alcohol protecting group; and wherein L1 is α-R3:β-R4, α-R4:β-R3, or a mixture of α-R3:β-R4 and α-R4:β-R3, wherein R3 and R4 are hydrogen, methyl, or fluoro, being the same or different, with the proviso that one of R3 and R4 is fluoro only when the other is hydrogen or fluoro.
2. The stereoselectively produced isomeric compound of claim 1, wherein Z is O, n is 1, X is COOH, Y1 is -CH2CH2- M1 is α-OH:β-R5, wherein R5 is hydrogen, L1 is α-R3:β-R4, wherein R3 and R4 are hydrogen and R7 is butyl.
3. A steroselectively produced isomeric compound according to the following formula: that is produced by a process for making 9-deoxy-PFG1-type compounds, the process comprising cyclizing a starting compound of the formula: into a compound of the following formula: by intramolecular cyclization of the enyne, wherein Z is O, S, CH2, or NR8 in which R8 is H, alkyl or aryl; X is H, CN, OR9, or COOR9 in which R9 is H; wherein n is 0, 1, 2, or 3; wherein Y1 is trans-CH=CH-, cis-CH=CH-, -CH2(CH2)m-, or -C=C-; m is 1,2, or 3; wherein R1 is an alcohol protecting group; wherein R7 is (5) -CpH2p-CH3, wherein p is an integer from one to 5, inclusive, (6) phenoxy optionally substituted by one, two or three chloro, fluoro, trifluoromethyl, (C1-C3)alkyl, or (C1-C3)alkoxy, with the proviso that not more than two substituents are other than alkyl, with the proviso that R7 is phenoxy or substituted phenoxy, only when R3 and R4 are lydrogen or methyl, being the same or different, (7) phenyl, bnezyl, phenylethyl, or phenylpropyl optionally substituted on the aromatic ring by one, two or three chloro, fluoro, trifluoromethyl, (C1-C3)alkyl, or (C1-C3)alkoyx, with the proviso that not more than two substituents are other than alkyl, (8) cis-CH=CH-CH2-CH3, (9) -(CH2)2-CH(OH)-CH3, or (10) -(CH2)3-CH=C(CH3)2; wherein -C(L1)-R7 taken together is (11) (C6-C7)cycloalkyl optionally substituted by one to 3 (C1-C5) alkyl; (12) 2-(2-furyl)ethyl, (13) 2-(3-thienyl)ethoxy, or (14) 3-thienyloxymethyl; wherein M1 is α-OH:β-R5 or α-R5:β-OH or α-OR1:β-R5 or α-R5:β-OR1, wherein R5 is hydrogen or methyl and R1 is an alcohol protecting group; and wherein L1 is α-R3:β-R4, α-R4:β-R3, or a mixture of α-R3:β-R4 and α-R4:β-R3, wherein R3 and R4 are hydrogen, methyl, or fluoro, being the same or different, with the proviso that one of R3 and R4 is fluoro only when the other is hydrogen or fluoro.
4. A steroselectively produced isomeric compound in pharmacologically acceptable salt form according to the following formula: that is produced by process for making 9-deoxy-PGF1-type compounds, the process comprising cyclizing a starting compound of the formula: into a compound of the following formula: by intramolecular cyclization of the enyne, wherein Z is O, S, CH2, or NR8 in which R8 is H, alkyl or aryl; X is H, CN, OR9, or COOR9 in which R9 is a pharmacologically acceptable cation; wherein n is 0, 1, 2, or 3; wherein Y1 is trans-CH=CH-, cis-CH=CH-, -CH2(CH2)m-, or -C=C-; m is 1,2, or 3; wherein R1 is an alcohol protecting group; wherein R7 is (1) -CpH2p-CH3, wherein p is an integer from one to 5, inclusive, (2) phenoxy optionally substituted by one, two or three chloro, fluoro, trifluoromethyl, (C1-C3)alkyl, or (C1-C3)alkoxy, with the proviso that not more than two substituents are other than alkyl, with the proviso that R7 is phenoxy or substituted phenoxy, only when R3 and R4 are hydrogen or methyl, being the same or different, (3) phenyl ,benzyl, phenylethyl, or phenylpropyl optionally substituted on the aromatic ring by one, tow or three chloro, fluoro, tribluoromethyl, (C1-C3)alkyl, or (C1-C3)alkoyx, with the proviso that not more than two substituents are other than alkyl, (4) cis-CH=CH-CH2-CH3, (5) -(CH2)2-CH(OH)-CH3, or (6) -(CH2)3-CH=C(CH3)2; wherein -C(L1)-R7 taken together is (1) (C4-C7)cycloalkyl optionally substituted by one to 3 (C1-C5) alkyl; (2) 2-(2-furyl)ethyl, (3) 2-(3-thienyl)ethoxy, or (4) 3-thienyloxymethyl; wherein M1 is α-OH:β-R4 or α-R5:β-OH or α-OR1:β-R5 or α-R5:β-OR1, wherein R5 is hydrogen or methyl and R1 is an alcohol protecting group; and wherein L1 is α-R3:β-R4, α-R4:β-R3, or a mixture of α-R3:β-R4 and α-R4:β-R3, wherein R3 and R4 are kydrogen, methyl, or fluoro, being the same or different, with the proviso that one of R3 and R4 is fluoro only when the other is hydrogen or fluoro.

Parent Case Info

This application is a divisional of U.S. patent application Ser. No. 09/541,521, filed Apr. 3, 2000, now U.S. Pat. No. 6,441,245, which is a continuation-in-part of U.S. patent application Ser. No. 09/481,390, filed Jan. 12, 2000, now abandoned, which is a continuation of U.S. patent application Ser. No. 08/957,736, filed Oct. 24, 1997, now abandoned.

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Foreign Referenced Citations (2)

Number	Date	Country
0087237	Aug 1983	EP
WO 9839337	Sep 1998	WO

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Continuations (1)

	Number	Date	Country
Parent	08/957736	Oct 1997	US
Child	09/481390		US

Continuation in Parts (1)

	Number	Date	Country
Parent	09/481390	Jan 2000	US
Child	09/541521		US

Process for stereoselective synthesis of prostacyclin derivatives

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