Claims
- 1. An isolated acylase enzyme obtained from Streptomyces anulatus No. 4811 or Streptomyces anulatus No. 8703, wherein said enzyme catalyzes deacylation of the acyl group R1 of a cyclic lipopeptide compound of the following general formula I or a salt thereof: whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl; to produce a cyclic peptide compound of the following general formula [II] or a salt thereof: wherein R2, R3, R4, R5, and R6 are as defined above; wherein said enzyme deacylates at an optimal pH of 8.0, at an optimal temperature of 50° C., and is activated in a concentration-dependent manner by methanol, wherein activation increases up to 10% by volume concentration of methanol, wherein the enzyme does not deacylate the compound FR901469.
- 2. The enzyme of claim 1, wherein said cyclic lipopeptide compound is selected from the group consisting of FR901379, Echinocandin B, and Aculeacin A.
- 3. An isolated enzyme obtained from Streptomyces No. 6907, comprising a large peptide and a small peptide wherein said large peptide comprises SEQ ID NO:1 and said small peptide comprises SEQ ID NO:2, wherein said enzyme catalyzes the deacylation of the lipid acyl moiety of a cyclic lipopeptide at an optimal pH of 9.0, an optimal temperature of 50° C., and is activated in a concentration-dependent manner by methanol, wherein activation increases up to 10% by volume concentration of methanol, and wherein the enzyme does not deacylate the compound FR901469.
- 4. The enzyme of claim 3, wherein said large peptide is approximately 61 kD and said small peptide is approximately 19 kD as determined by SDS-PAGE.
- 5. The enzyme of claim 3, wherein the enzyme deacylates a compound selected from the group consisting of FR901379, Echinocandin B, and Aculeacin A.
- 6. The acylase of claim 3 which catalyzes deacylation of the acyl group R1 of a cyclic lipopeptide compound of the following general formula I or a salt thereof: whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl; to produce a cyclic peptide compound of the following general formula [II] or a salt thereof: wherein R2, R3, R4, R5, and R6 are as defined above.
- 7. The acylase according to claim 6, whereinR5 is hydroxysulfonyloxy and R6 is carbamoyl.
- 8. A method of producing a cyclic peptide compound of the formula [II] wherein R2, R1, R4, R5, and R6 are the same groups as respectively defined below or a salt thereof, which comprises contacting a cyclic lipopeptide compound of the following general formula [I]: whereinR1 is acyl; R2 is hydroxy or acyloxy; R3 is hydrogen or hydroxy; R4 is hydrogen or hydroxy; R5 is hydrogen or hydroxysulfonyloxy; and R6 is hydrogen or carbamoyl or a salt thereof , in an aqueous solvent with the acylase of claim 3.
- 9. The method according to claim 8, whereinR5 is hydroxysulfonyloxy and R6 is carbamoyl.
Priority Claims (2)
Number |
Date |
Country |
Kind |
8-051386 |
Mar 1996 |
JP |
|
8-194207 |
Jul 1996 |
JP |
|
Parent Case Info
This Appln. is a 371 of PCT/JP97/00000 Mar. 6, 1997.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/JP97/00692 |
|
WO |
00 |
9/3/1998 |
9/3/1998 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO97/32975 |
9/12/1997 |
WO |
A |
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4396543 |
Debono |
Aug 1983 |
|
5573936 |
Kreuzman |
Nov 1996 |
|
Non-Patent Literature Citations (1)
Entry |
Boeck et al., J. Antibiotics, 1988, vol. 41, No. 8, pp. 1085-1092. |