Claims
- 1. A process for preparing the compund of Formula I; ##STR10## and its minor related components A, B, C and D which comprises the aerobic fermentation of a strain of Aspergillus alliaceus Thom and Church that is capable of producing said compound and its minor related components A, B, C and D wherein said aerobic fermentation is under operating conditions and a time period that are controlled so as to produce a recoverable amount of said compound or one of its minor related components A, B, C, and D in an assimilable aqueous nutrient medium and isolating at least one of said compound and its minor related components A, B, C and D.
- 2. The process of claim 1 wherein the controlled aerobic fermentation is at a temperature range of from 20.degree. to 37.degree. C. for at least 24 hours.
- 3. The process of claim 1 wherein the strain of Aspergillus alliaceus Thom and Church is selected from the group consisting of ATCC No. 20655 and ATCC No. 20656.
- 4. The process of claim 1 wherein the controlled aerobic fermentation is at 28.degree. C. for about 144 hours.
- 5. The process of claim 1 wherein the isolation of the compound of Formula I is accomplished by an extraction step followed by a chromatographic separation step.
- 6. The process of claim 5 wherein the chromatographic step comprises the sequential chromatographic steps of:
- (a) silica gel chromatography;
- (b) gel permeation chromatography;
- (c) reverse phase high pressure liquid chromatography.
Parent Case Info
This is a continuation of application Ser. No. 509,883, filed July 1, 1983, now U.S. Pat. No. 4,530,790, now U.S. Pat. No. 4,530,790, which in turn is a continuation-in-part of application Ser. No. 453,383, filed Dec. 27, 1982, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
3944470 |
Diehl et al. |
Mar 1976 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
0722946 |
Mar 1980 |
SUX |
Non-Patent Literature Citations (1)
Entry |
Chien et al.; "Microbial Transformations of Natural Antitumor Agents: Use of Solubilizing Agents to Improve Yields of Hydroxylated Ellipticines"; Applied and Environmental Microbiology, vol. 40, No. 4, Oct. 1980, pp. 741-745. |
Continuations (1)
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Number |
Date |
Country |
Parent |
509883 |
Jul 1983 |
|
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
453383 |
Dec 1982 |
|