Claims
- 1. A method for converting a nitrile in an R, S mixture of nitriles of the formula ##STR8## wherein: A is selected from the group consisting of: ##STR9## R.sup.1 is C.sub.1 -C.sub.4 alkyl; R.sup.2 is H or OH;
- R.sup.3 is H, Cl, OCF.sub.2 H, (CH.sub.3).sub.2 CHCH.sub.2, H.sub.2 C.dbd.C(CH.sub.3)CH.sub.2 NH, ##STR10## R.sup.4 is Cl or F; to the corresponding amide comprising contacting said nitrile mixture with a biological material that stereospecifically converts the R- or S-nitrile in said mixture to the corresponding enantiomeric R or S-amide, the biological material being located in or obtained from Pseudomonas putida, Pseudomonas aureofaciens or Serratia liquefaciens.
- 2. A method for converting a nitrile in an R, S mixture of nitriles of the formula ##STR11## wherein: A is selected from the group consisting of: ##STR12## R.sup.1 is C.sub.1 -C.sub.4 alkyl; R.sup.2 is H or OH;
- R.sup.3 is H, Cl, OCF.sub.2 H, (CH.sub.3).sub.2 CHCH.sub.2, H.sub.2 C.dbd.C(CH.sub.3)CH.sub.2 NH, ##STR13## R.sup.4 is Cl or F; to the corresponding amide comprising contacting said nitrile mixture with a biological material that stereospecifically converts the R- or S-nitrile in said mixture to the corresponding enantiomeric R or S-amide, the biological material being located in or obtained from Pseudomonas putida 13-5S-ACN-2a, Pseudomonas putida 5B-MGN-2P, Pseudomonas putida 2D-11-5-1b, Pseudomonas sp. 20-5-MGN-1P, Pseudomonas sp. 3L-B-2-6-1P, Pseudomonas sp. 3L-G-2-5-1a, Pseudomonas sp. 3L-G-1-5-1a, Pseudomonas sp. 5A-MGN-1P, Pseudomonas sp. 5B-ACN-1P, Pseudomonas sp. 2G-8-5-2, Pseudomonas sp. 2G-8-5-1, Pseudomonas sp. 2D-11-5-1, Pseudomonas sp. 2D-11-5-1c, Pseudomonas sp. 20-5-SBN-1b, Pseudomonas aureofaciens MOB C2-1, Moraxella sp. 3L-A-1-5-1a-1, Serratia liquefaciens MOB IM/N3 or strain 3L-G-1-2-1a.
- 3. The method according to claim 1 or 2 wherein A is selected from the group ##STR14## R.sup.1 is selected from CH.sub.3 and CH(CH.sub.3).sub.2.
- 4. The method according to claim 2 wherein A is selected from the group ##STR15##
- 5. The method according to claim 4 wherein the nitrile is selected from the group (2-(4-chlorophenyl)-3-methylbutyronitrile, 2-(4-isobutylphenyl)propionitrile and 2-(6-methoxy-2-naphthyl)-propionitrile.
- 6. The method according to claim 5 wherein the nitrile is selected from the group 2-(4-chlorophenyl)-3-methylbutryonitrile and 2-(6-methoxy-2-naphthyl)propionitrile.
- 7. The method of claim 1 wherein the biological material is located in or obtained from Pseudomonas putida or Serratia liquefaciens.
- 8. The method according to claim 7 wherein the biological material is located in or obtained from Pseudomonas putida.
- 9. The method according to claim 8 wherein the biological material is located in or obtained from Moraxella sp 3L-A-1-5-1A-1.
- 10. The method according to claim 7 wherein the biological material is located in or obtained from Serratia liquefaciens.
CROSS REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of U.S. Ser. No. 08/030,015 filed Mar. 22, 1993, which in turn is a continuation-in-part of U.S. Ser. No. 07/585,554 filed Sep. 20, 1990 both now abandoned.
US Referenced Citations (13)
Foreign Referenced Citations (5)
Number |
Date |
Country |
0326482 |
Aug 1989 |
EPX |
0330529 |
Aug 1989 |
EPX |
0348901 |
Jan 1990 |
EPX |
0356912 |
Mar 1990 |
EPX |
WO8607386 |
Dec 1986 |
WOX |
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
30015 |
Mar 1993 |
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Parent |
585554 |
Sep 1990 |
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