Claims
- 1. A method for the isolation of immunomodulatory carbohydrate from Aloe species, comprising:
(a) extracting Aloe gel juice from said Aloe species; (b) performing a controlled enzymatic limited hydrolysis of total polysaccharide in said Aloe gel juice at a temperature and for a period of time suitable for controlled limited carbohydrate hydrolysis, wherein the immunomodulatory activity is maximized; (c) terminating said controlled limited hydrolysis; (d) optionally decolorizing and filtering said hydrolyzed Aloe gel juice; and (e) purifying said decolorized and filtered hydrolyzed Aloe gel juice via nanofiltration.
- 2. The method of claim 1 wherein said enzyme is selected from the group consisting of cellulase, pectinase or mannanase.
- 3. The method of claim 1 wherein said enzymatic hydrolyzing agent is cellulase, added at a ratio of 0.5 g -2.5 g of cellulase to 216 L of aloe gel juice.
- 4. The method of claim 3 wherein step (b) is performed at 25° C. for 2-2.5 hours.
- 5. The method of claim 1 wherein said hydrolysis is terminated by heating or by neutralization.
- 6. The method of claim 5 wherein said hydrolysis is terminated by heating to 85-90° C. for 30-50 minutes.
- 7. The method of claim 1 wherein step (d) is accomplished by adding charcoal to said Aloe gel juice and then passing said Aloe gel juice through a series of filters with progressively smaller pore sizes.
- 8. The method of claim 7 wherein said series of filters comprises a 30 μm filter, a 1 μm filter and a 0.7 μm filter.
- 9. The method of claim 8 further comprising the addition of a diatomaceous earth material selected from the group consisting of celite, FW12, or FW14 as a filtration aid to said Aloe gel juice in step (c).
- 10. The method of claim 1 further comprising optionally repeating step (e).
- 11. A method for the isolation of immunomodulatory carbohydrate composition, wherein said composition is comprised of:
(i) primarily (>95%) of polysaccharides derived from Aloe, said polysaccharides in said composition having an average molecular weight of 70-80 kDa with a range between 50-200 kDa; and (ii) said polysaccharides are comprised of D-galactose (approx. 5% or less), D-glucose (approx. 5% or less) and D-mannose (approximately 90%); said method comprising:
(a) extracting Aloe gel juice from said Aloe species; (b) performing a controlled limited hydrolysis of total polysaccharide in said Aloe gel juice at a temperature and for a period of time suitable for controlled limited carbohydrate hydrolysis, wherein the immunomodulatory activity is maximized; (c) terminating said controlled limited hydrolysis; and (d) optionally decolorizing and filtering said Aloe gel juice. (e) purifying said decolorized and filtered hydrolyzed aloe gel juice via nanofiltration.
- 12. The method of claim 11 wherein step (b) is accomplished by treating said Aloe gel juice with an enzymatic or chemical hydrolyzing agent.
- 13. The method of claim 12 wherein said enzyme is selected from the group consisting of cellulase, pectinase or mannanase.
- 14. The method of claim 12 wherein said enzymatic hydrolyzing agent is cellulase, added at a ratio of 0.5 g-2.5 g of cellulase to 216 L of aloe gel juice.
- 15. The method of claim 14 wherein step (b) is performed at 25° C. for 2-2.5 hours.
- 16. The method of claim 11 wherein said hydrolysis is terminated by heating or by neutralization.
- 17. The method of claim 16 wherein said hydrolysis is terminated by heating to 85-90° C. for 30-50 minutes.
- 18. The method of claim 11 wherein step (d) is accomplished by adding charcoal to said Aloe gel juice and then passing said Aloe gel juice through a series of filters with progressively smaller pore sizes.
- 19. The method of claim 18 wherein said series of filters comprises a 30 μm filter, a 1 μm filter and a 0.7 μm filter.
- 20. The method of claim 18 further comprising the addition of a diatomaceous earth material selected from the group consisting of celite, FW12, or FW14 as a filtration aid to said Aloe gel juice in step (c).
- 21. The method of claim 11 further comprising optionally repeating step (e).
RELATED APPLICATIONS
[0001] This application is a continuation in part of U.S. patent application Ser. No. 09/295,907, filed Apr. 21, 1999, which is a divisional of U.S. patent application Ser. No. 09/169,449, filed Oct. 9, 1998, now U.S. Pat. No. 6,133,440, which claims the benefit of U.S. Provisional Patent Application Serial No. 60/061,681, filed Oct. 10, 1997 and U.S. Provisional Patent Application Serial No. 60/098,271, filed Aug. 28, 1998, each of which is entitled “Process for Preparation of Immunomodulatory Carbohydrates from Aloe.”
Provisional Applications (2)
|
Number |
Date |
Country |
|
60061681 |
Oct 1997 |
US |
|
60098271 |
Aug 1998 |
US |
Divisions (1)
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Number |
Date |
Country |
Parent |
09169449 |
Oct 1998 |
US |
Child |
09295907 |
Apr 1999 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09295907 |
Apr 1999 |
US |
Child |
10039752 |
Jan 2002 |
US |