Claims
- 1. In a process of preparing L-serine from glycine and formaldehyde in the presence of a harvested microorganism or the cell extract thereof having a serine hydroxymethyl transferase activity, the improvement which comprises pretreating Escherichia coli MT-10350 FERM BP-793, Escherichia coli MT-10351 FERM BP-794 or the cell extract of either of the Escherichia coli strains in a suspension or solution form, under conditions of dissolved oxygen of 1 ppm or more and a temperature of 60.degree. C. or less prior to the addition of the reaction mixture.
- 2. The process of preparing L-serine according to claim 1, wherein the temperature of 60.degree. C. or less in the pre-treatment is 30.degree. to 50.degree. C.
- 3. The process of preparing L-serine according to claim 1, wherein the dissolved oxygen of 1 ppm or more in the pre-treatment is attained by passing air or oxygen through the suspension or solution.
- 4. The process of preparing L-serine according to claim 1, wherein the pre-treatment is carried out while agitating the suspension or solution.
- 5. The process of preparing L-serine according to claim 1, wherein the pre-treatment is carried out for 2 to 10 hours.
- 6. The process of preparing L-serine according to claim 1, wherein the pre-treatment is carried out at pH 6 to 9.
- 7. The process of preparing L-serine according to claim 1, wherein the pre-treatment is carried out in the presence of an anti-foaming agent.
- 8. The process of preparing L-serine according to claim 1, wherein the pre-treatment is carried out in the presence of glycine.
- 9. The process of preparing L-serine according to claim 1, wherein the formaldehyde is in a form selected from the group consisting of gas, aqueous solution, alcoholic solution and paraformaldehyde.
- 10. The process of preparing L-serine according to claim 1, wherein the glycine and the formaldehyde are reacted in the presence of a nitrogen gas or a reducing agent.
- 11. The process of preparing L-serine according to claim 1, wherein the glycine and the formaldehyde are reacted in a pH range of 6 to 9.
- 12. The process of preparing L-serine according to claim 1, wherein the glycine and the formaldehyde are reacted in a temperature range of 30.degree. to 60.degree. C.
- 13. The process of preparing L-serine according to claim 1, wherein the glycine and the formaldehyde are reacted for 20 to 30 hours.
Priority Claims (1)
Number |
Date |
Country |
Kind |
1-260203 |
Oct 1989 |
JPX |
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RELATED APPLICATIONS
This application is a continuation of U.S. Ser. No. 07/592,508, filed Oct. 3, 1990, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4782021 |
Ishiwata et al. |
Nov 1988 |
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Foreign Referenced Citations (1)
Number |
Date |
Country |
0185824 |
Jul 1986 |
EPX |
Non-Patent Literature Citations (2)
Entry |
Chemical Abstracts 107:234706q "Efficient conversion of glycine to L-serine by a glycine-resistant mutant of a methylotroph using cobalt(2+) as an inhibitor of L-serine degradation". |
Patent Abstracts of Japan, vol. 7, No. 242 (Oct. 27, 1983) "Method for Suppressing Activity of Serine Decomposing Enzyme in Bacterial Cell". |
Continuations (1)
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Number |
Date |
Country |
Parent |
592508 |
Oct 1990 |
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