Claims
- 1. A method for the determination of an analyte selected from the group consisting of an acid, base, salt, enzyme, inorganic or organic catalyst and electron donor comprising reacting the analyte with a compound of formula III, ##STR9## in which R represents ##STR10## and in which R.sub.1 and R.sub.2 are the same or different and represent hydrogen or lower alkyl C.sub.1 -C.sub.6 which is straight-chained or branched, X is a cleavable group and at least one of the groups R.sub.4 or R.sub.5 represents a group stabilizing the dioxetan structure selected from the group consisting of adamanyl, phenyl, cyclohexyl and a secondary or tertiary alkyl residue or at most one of the groups R.sub.4 or R.sub.5 represents hydrogen; and cleaving the group X and measuring the emitted light as a measure for the content of the analyte to be determined.
- 2. A chemiluminescent method for determining an analyte in an immunological assay comprising reacting the analyte with a compound of formula III, ##STR11## in which R represents ##STR12## and in which R.sub.1 and R.sub.2 are the same or different and represent hydrogen or lower alkyl C.sub.1 -C.sub.6 which is straight-chained or branched, X is a cleavable group and at least one of the groups R.sub.4 or R.sub.5 represents a group stabilizing the dioxetan structure selected from the group consisting of adamanyl, phenyl, cyclohexyl and a secondary or tertiary alkyl residue or at most one of the groups R.sub.4 or R.sub.5 represents hydrogen; and
- cleaving the group X after the immunological reaction of the ligand and the analyte and measuring the emitted light as a measure for the extent or presence of the immunologically determinable analyte.
- 3. A chemiluminescent method for determining an analyte in an immunological assay comprising reacting the analyte with a compound of formula III, ##STR13## in which R represents ##STR14## and in which R.sub.1 and R.sub.2 are the same or different and represent hydrogen or lower alkyl C1-C6 which is straight-chained or branched, X is a clearable group and at least one of the groups R.sub.4 or R.sub.5 represents a group stabilizing the dioxetan structure selected from the group consisting of adamanyl, phenyl, cyclohexyl and a secondary or tertiary alkyl residue or at most one of the groups R.sub.4 or R.sub.5 represents hydrogen; and
- cleaving the group X after the immunological reaction of the ligand and the analyte and measuring the emitted light as a measure for the extent or presence of the immunologically determinable analyte.
Priority Claims (1)
Number |
Date |
Country |
Kind |
42 10 759.8 |
Jan 1992 |
DEX |
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Parent Case Info
This is a Divisional Application of application Ser. No. 08/150,188, filed Nov. 30, 1993 now U.S. Pat. No. 5,455,357.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5068339 |
Schapp |
Nov 1991 |
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Divisions (1)
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Number |
Date |
Country |
Parent |
150188 |
Nov 1993 |
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