Claims
- 1. A process for the purification of plasminogen activator inhibitor 2 (PAI-2), which comprises the steps:
- (a) preincubating a PAI-2-containing solution with a compound which cleaves disulfide linkages;
- (b) mixing said solution with a water-soluble acridine or quinoline base to precipitate impurities in the solution; and
- (c) separating the precipitated impurities to obtain PAI-1 in purified form.
- 2. The process as claimed in claim 1, wherein the PAI-2-containing solution is an extract of placenta or a solution of genetically engineered PAI-2.
- 3. The process as claimed in claim 1, wherein the disulfide-cleaving compound is dithiothreitol.
- 4. The process as claimed in claim 1, wherein the disulfide-cleaving compound is used in a concentration of from 10 mmol/l to about 250 mmol/l.
- 5. The process as claimed in claim 1, wherein preincubation is carried out for 15 min to about 3.
- 6. The process as claimed in claim 1, wherein said preincubation is carried out at about 10.degree. to about 40.degree. C.
- 7. The process as claimed in claim 1, wherein the base is used in dissolved form at a pH of about 5.5 to about 8.5.
- 8. The process as claimed in claim 1, wherein said PAI-2-containing solution is pasteurized.
- 9. The process according to claim 1, wherein said base is 2-ethoxy-6,9-diaminoacridine lactate.
- 10. The process according to claim 1,
- wherein separating the precipitated impurities is carried out by filtering, and
- isolating the PAI-2 from the filtrate.
- 11. The process according to claim 4, wherein the disulfide-cleaving compound is employed in a concentration of about 100 mmol/l.
- 12. The process according to claim 5, wherein said preincubation is carried out at about 37.degree. C.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3929504 |
Sep 1989 |
DEX |
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Parent Case Info
This application is a continuation of application Ser. No. 07/719,078, filed Jun. 19, 1991, now abandoned, which is a continuation of application Ser. No. 07/577,631, filed on Sep. 4, 1990, now abandoned.
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WOX |
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Entry |
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Continuations (2)
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Number |
Date |
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Parent |
719078 |
Jun 1991 |
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Parent |
577632 |
Sep 1990 |
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