Processes for purifying oligosaccharides

Abstract

Processes for purifying oligosaccharides including contacting a basic oligosaccharide solution with a basic boronate resin to bind monosaccharides to the basic boronate resin and obtain a purified oligosaccharide solution. The basic boronate resin can bind about 50% of the monosaccharides and about 10% of oligosaccharides in the basic oligosaccharide solution, by weight of total carbohydrates therein.

Description

Claims

1. A process for purifying oligosaccharides comprising: contacting a basic oligosaccharide solution with a basic boronate resin to bind monosaccharides to the basic boronate resin and obtain a purified oligosaccharide solution,

2. The process of claim 1 wherein the boronate resin comprises a boronate derivative selected from the group consisting of ortho-aminophenyl boronate, meta-aminophenyl boronate, para-aminophenyl boronate and combinations thereof.

3. The process of claim 1 wherein the oligosaccharides comprise two or more sugars selected from the group consisting of glucose, fructose, galactose, xylose and combinations thereof.

4. The process of claim 1 wherein the oligosaccharides are selected from the group consisting of fructooligosaccharide, galactooligosaccharide, lactosucrose, isomaltulose, glycosyl sucrose, isomaltooligosaccharide, gentioligosaccharide, xylooligosaccharide and combinations thereof.

5. The process of claim 1 wherein the basic oligosaccharide solution and the basic boronate resin each comprise a pH of at least about 7.5.

6. The process of claim 1 wherein the basic boronate resin binds at least about 75% of the monosaccharides and less than about 10% of the oligosaccharides in the basic oligosaccharide solution, by weight of total carbohydrates.

7. The process of claim 1 wherein the basic boronate resin binds from about 90% to about 99.9% of the monosaccharides and from about 0.1% to about 5% of the oligosaccharides in the basic oligosaccharide solution, by weight of total carbohydrates.

8. The process of claim 1 comprising drying the purified oligosaccharide solution to obtain a purified oligosaccharide composition.

9. The process of claim 1 comprising: regenerating the basic boronate resin comprising bound monosaccharides by contacting an acid with the basic boronate resin to obtain an acidic boronate resin; andseparating the bound monosaccharides from the acidic boronate resin.

10. The process of claim 9 wherein the acid is selected from the group consisting of mineral acids, organic acids, ionized water with H+ and combinations thereof.

11. The process of claim 8 wherein the purified oligosaccharide composition comprises at least about 75% oligosaccharides by weight of total carbohydrates.

12. A process for purifying oligosaccharides comprising: providing a crude oligosaccharide solution and a boronate resin;adjusting pH of the crude oligosaccharide solution to obtain a basic oligosaccharide solution;adjusting pH of the boronate resin to about the pH of the basic oligosaccharide solution to obtain a basic boronate resin; andcontacting the basic oligosaccharide solution with the basic boronate resin to bind monosaccharides to the basic boronate resin and obtain a purified oligosaccharide solution

13. The process of claim 16 comprising treating the crude oligosaccharide solution with a catalytic enzyme selected from the group consisting of α-glucosidase, β-galactosidase and combinations thereof.

14. The process of claim 16 comprising adjusting the pH of the crude oligosaccharide solution to at least about 7.5 by adding a base selected from the group consisting of sodium hydroxide, calcium hydroxide, ammonium hydroxide, potassium hydroxide, alkaline water, ionized water with OH− and combinations thereof.

15. The process of claim 16 wherein the boronate resin comprises a boronate derivative selected from the group consisting of ortho-aminophenyl boronate, meta-aminophenyl boronate, para-aminophenyl boronate and combinations thereof.

16. The process of claim 16 wherein the oligosaccharides comprise two or more sugars selected from the group consisting of glucose, fructose, galactose, xylose and combinations thereof.

17. The process of claim 16 wherein the basic boronate resin binds at least about 75% of the monosaccharides and less than about 10% of the oligosaccharides in the basic oligosaccharide solution, by weight of total carbohydrates.

18. The process of claim 16 comprising adjusting the pH of the boronate resin to at least about 7.5 using a base selected from the group consisting of sodium hydroxide, calcium hydroxide, ammonium hydroxide, potassium hydroxide, alkaline water, ionized water with OH− and combinations thereof.

19. The process of claim 16 wherein the basic boronate resin binds from about 90% to about 99.9% of the monosaccharides and from about 0.1% to about 5% of the oligosaccharides in the basic oligosaccharide solution, by weight of total carbohydrates.

20. A process for purifying oligosaccharides comprising: contacting a basic oligosaccharide solution having a pH of from about 8 to about 10 with a basic boronate resin having a pH of from about 8 to about 10 to bind monosaccharides to the basic boronate resin and obtain a purified oligosaccharide solution,