Processing of whole fruits and vegetables, processing of side-stream ingredients of fruits and vegetables, and use of the processed fruits and vegetables in beverage and food products

Information

  • Patent Grant
  • 10334870
  • Patent Number
    10,334,870
  • Date Filed
    Friday, September 30, 2011
    13 years ago
  • Date Issued
    Tuesday, July 2, 2019
    5 years ago
Abstract
Processing and use of whole fruits and vegetables or side-stream ingredients of juice extraction, paste, or ketchup process, or canning industry, in particular, the processing of the by-products, including pomace, and its use in beverage and food products.
Description
FIELD OF THE INVENTION

The invention relates to processing and use of whole fruits and vegetables and to processing of side-stream ingredients of juice extraction and paste production. More particularly, the invention relates to methods of reducing the particle size of whole fruits and vegetables or side-stream ingredients thereof. The invention further relates to use of the processed whole fruits and vegetables and use of the processed side-stream ingredients in beverage and food products.


BACKGROUND OF THE INVENTION

Whole vegetables and fruits are generally not used in beverages as they contain ingredients such as skins, seeds, stems, roots, and the like which may affect the taste and mouthfeel of the beverage. Instead, juices and/or pulp are obtained from the vegetables and fruits and the skins, seeds, stems, roots, and the like are discarded. These discarded ingredients are often in the form of pomace.


Pomace is the pulpy edible by-product remaining after fruit or vegetable juice pressing processes, wine crush operations, puree and concentrate operations, canning processes, and other food manufacturing processes. Pomace may include, for example, skins, pulp, seeds, and edible part of stems of the fruit and vegetable such as apples or carrots. In some cases the pomace can derive or contain other parts of the fruit and vegetable such as pod, stalk, flower, root, leaves and tuber. In a juice extraction process, the pomace is typically in the form of a part of press cake. By-products from paste and puree processes such as tomato skins and seeds from tomato ketchup and paste processing are also included in the pomace even they are not the by-products from juice extracts. Fruit skins from cannery are also edible by-products. Hereafter, pomace includes all edible by-products from fruit and vegetable juice, paste, puree and canning processes.


Pomace has been used for fertilizer and substrates for microorganism growth, for example, and dried pomace has had limited use in food products such as soups and snacks, and also has been used in the dietary supplement industry. However, pomace is generally not used in food products due to its gritty texture, sedimentation, fibrous nature, high insoluble fiber content, intense flavor and lack of starch and protein. Thus pomace is generally considered a waste by-product in the fruit and vegetable industry.


For example, cranberry pomace remains after the squeezing of juice for cranberry type cocktails and juices and concentrates. These cranberry pomace materials have been found to contain 70% to 75% fiber with in insoluble to soluble fiber ratio of 9 or 10 to 1 (wet basis) less than 5%-10% protein, and less that 5% sugars and starches. Thus, by-products are typically removed from the juicing process due to challenges with texture and flavor when creating a juice beverage.


It would be beneficial to process pomace as well as whole fruits and vegetables to arrive at a useful food ingredient in order to avoid such waste.


BRIEF SUMMARY OF THE INVENTION

In one aspect, the invention relates to whole fruits and vegetables which have been processed to provide a product having particle and fiber size less than 250 microns and their use in beverage and food products.


In another aspect, the invention relates to a method of processing whole fruits and vegetables to provide a product having particle and fiber size less than 250 microns to improve texture and dispersion properties.


In another aspect, the invention relates to side-stream ingredients (pomace) having particle and fiber size less than 250 microns and its use in beverage and food products.


In another aspect, the invention relates to a method of reducing the particle and fiber particle size of pomace to less than 250 microns to improve texture and dispersion properties.


In another aspect, the invention relates to a method of reducing the particle and fiber size of pomace wherein the pomace is hydrated and frozen prior to micro-grinding.


In another aspect, the invention relates to pre-treating the whole fruits and vegetables or pomace with heat prior to AOAC fiber analysis to maximize fiber detection.







DETAILED DESCRIPTION OF THE INVENTION

Side-stream ingredients (hereinafter “pomace”) generally contains high total dietary fiber content (50 or more percent by weight), low amounts of in sugars typically less than 5% but more commonly less than 2% by weight in wet pomace, and varying amounts of essential vitamins, minerals and phytonutrients (depending on types of fruit/vegetable and process applied). Likewise, whole fruits and vegetables also contain fiber and nutrient content much higher than the extracted juice products and thus are much healthier.


Whole Fruits and Vegetables


Whole vegetables and fruits include juices, pulp, skins, seeds, roots, and the like. Aspects of the invention utilize the entire vegetable or fruit to prepare a product for consumption either alone or in combination with other ingredients. In accordance with the present invention, the desired product has a reduced particle size of less than 250 microns. The whole fruits and vegetables are pre-treated prior to size reduction by, for example, crushing, cutting, pulverizing, or homogenizing to form an intermediate product. This intermediate product is then subjected to particle (including fiber) size reduction as discussed in more detail below.


Prior to particle size reduction, the intermediate product may be acidified to lower the pH. Typically the product is acidified to under pH 4.5 for microbial stability. Sufficient amounts of organic acids such as citric acid, lactic acid, acetic acid, malic acid, succinic acid, or juices such as lemon juice and/or acidic juice concentrate may be added to the product to reduce the pH of product to a desired pH.


Whole fruits and vegetables any be any suitable fruit or vegetable such as, but not limited to, carrot, cranberry, orange, blueberry, tomato, apple, and any combination thereof. Lemons, limes, grapes, strawberries, grapefruits, tangerine, mandarin orange, tangelo, pomelo, celery, beet, lettuce, spinach, cabbage, artichoke, broccoli, brussels sprouts, cauliflower, watercress, peas, beans, lentils, asparagus, onions, leeks, kohlrabi, radish, turnip, rutabaga, rhubarb, carrot, cucumber, zucchini, eggplant, pineapple, peach, banana, pear, guava, apricot, watermelon, Saskatoon berry, blueberry, plains berry, prairie berry, mulberry, elderberry, Barbados cherry (acerola cherry), choke cherry, date, coconut, olive, raspberry, strawberry, huckleberry, loganberry, currant, dewberry, boysenberry, kiwi, cherry, blackberry, quince, buckthorn, passion fruit, rowan, gooseberry, pomegranate, persimmon, mango, papaya, lychee, plum, prune, fig, or their combination.


Pomace (Side-Stream Ingredients)


Pomace a natural by-product from the extraction of juices from fruits and vegetables and other processes. Pomace contains natural nutrients (such as vitamin A, vitamin C, vitamin E, anthocyanins, and antioxidants), flavors, and/or colors of the original fruits and vegetables. Pomace also contains high natural (un-processed) fibers. Most pomace contains over 50% (d.b.) of dietary fiber, mostly insoluble fiber. The use of pomace in food products will fortify fiber and naturally existing nutrients such as vitamins and antioxidants. Hence the use of pomace provides an excellent natural and label-friendly way to enhance quality of food products including, for example, beverages, soups, spreads, puddings, smoothies and snacks. Moreover, pomace is generally a low cost ingredient because it is under-utilized and is considered a waste from juice industry.


Pomace may be obtained from any suitable fruit or vegetable such as, but not limited to, carrot, cranberry, orange, blueberry, tomato, apple, and any combination thereof. Lemons, limes, grapes, strawberries, grapefruits, tangerine, mandarin orange, tangelo, pomelo, celery, beet, lettuce, spinach, cabbage, artichoke, broccoli, brussels sprouts, cauliflower, watercress, peas, beans, lentils, asparagus, onions, leeks, kohlrabi, radish, turnip, rutabaga, rhubarb, carrot, cucumber, zucchini, eggplant, pineapple, peach, banana, pear, guava, apricot, watermelon, Saskatoon berry, blueberry, plains berry, prairie berry, mulberry, elderberry, Barbados cherry (acerola cherry), choke cherry, date, coconut, olive, raspberry, strawberry, huckleberry, loganberry, currant, dewberry, boysenberry, kiwi, cherry, blackberry, quince, buckthorn, passion fruit, rowan, gooseberry, pomegranate, persimmon, mango, papaya, lychee, plum, prune, fig, or their combination. In particular, pomace obtained from orange, cranberry, and carrot provides valuable nutrients and fiber.


Pomace may be obtained from any processing method that produces side-stream ingredients. For example pomace may be obtained and used as is from the press cake after a juice extraction process; however, such pomace generally has a wide range of particle and fiber sizes with a significant amount being large particles. For example, particle or fiber sizes of pomace may range from 100 microns to 5 cm depending upon the particular type of extracted fruit or vegetable. Pomace containing larger particles and fiber, and/or a wide distribution of particle or fiber sizes does not have a smooth texture. Therefore, mixing such pomace into beverage or snack formulations provides a tough, sandy, and gritty texture. Such pomace also causes a non-homogeneous dispersion in liquid-based products. For ease of discussion, the term “particle size” refers to both particle size and fiber size.


As used herein, pomace may also include the “goo” or the retentate formed from a filtration step of an extraction process, e.g., when clarifying a juice. This filtration retentate may be obtained as a by-product, for example, from any suitable fruit or vegetable juice such as carrot or cranberry juices. This retentate or goo may be added to pomace obtained from the press cake, for example, and is hereinafter is collectively referred to as pomace. Pomace (including filtration retentate) “as is” provides fiber, color, nutrients, mouthfeel, flavor.


It was discovered that the pomace may be treated to reduce the particle (including fiber) size resulting in improved texture and dispersion capability of the pomace in a product. It was further discovered that upon size reduction, the ratio of soluble portions of the pomace to insoluble portions increases, more flavors and colors are released, and the texture of the particles feels smoother.


Any suitable form of pomace such as wet pomace, frozen pomace, or dry pomace may be used to obtain the desired product. Wet or frozen pomace does not require rehydration. Dry pomace is lighter and easier to store. Wet pomace may be taken directly as the press cake. The moisture content of the pomace depends on the fruit or vegetable they may vary on moisture content. There are various methods to dry pomace if desired, for example on a commercial scale a continuous forced air indirect fire oven may be used.


The pH of the pomace may range from 2 to 7. Prior to particle size reduction, the pomace may be acidified to lower the pH. Typically the pomace is acidified to under pH 4.5 for microbial stability. Sufficient amounts of organic acids such as citric acid, lactic acid, acetic acid, malic acid, succinic acid, or juices such as lemon juice and/or acidic juice concentrate may be added to the pomace to reduce the pH of pomace to a desired pH.


Processing of Whole Fruits and Vegetables and/or Pomace


In accordance with one aspect, the whole fruits or vegetables intermediate product obtained by pre-treating the whole fruits or vegetables as discussed above or pomace material is subjected to a size-reduction process. Size reduction may occur through any suitable mechanical or chemical process such as micro-grinding, cutting, shredding, slicing, grinding, shearing, extruding, homogenizing, pulverizing, comminuting, or subjecting to ultrasonic frequency. In one aspect, the size reduction is achieved by micro-grinding.


Micro-grinding process is typically used for pureeing portions of fruits and vegetables such as fruits and vegetables wherein the skin, seeds, etc. are removed, but has not heretofore been known to reduce the size of whole fruits and vegetables or of pomace.


This process comprises feeding, grinding, and mesh-screening or filtering processes in a unit operation. Therefore, the outcome has more controlled and homogeneous size distribution than random grinding without mesh-screening process. The micro-grinding process provides a product which is very suitable for addition to food products, and in particular beverage products to provide high nutritional value. For further size reduction, the micro-ground product is, optionally, passed through pressurized homogenizers such as dairy or dipping sauce homogenizers. The micro-ground product can be incorporated in wet form or dehydrated to appropriate moisture level prior to use in product formulation.


Ideally, the particle size of the whole fruits and vegetables or pomace is reduced to at least below 250 microns to provide a micro-ground product with a smooth texture and dispersibility, while below 125 micron is recommended for the better sensory result. In some examples, the particle size may be reduced to below 75 micron or below 38 micron. The range of 38 micron to 125 micron particularly improves both dispersion and mouthfeel characteristics in beverages. Micro-grinding also increases: (1) total fiber content; and (2) the ratio of soluble fiber to insoluble fiber. This indicates that the mechanical grinding method partially converts: (1) non-detectable fiber to detectable by AOAC method; and (2) insoluble fiber to soluble fiber. See Example 2 below.


In another aspect of the invention, pomace is prepared for freeze-shearing by adding water to the pomace. The amount of water depends on the type of pomace. For example, cranberry pomace requires a 20:1 water:pomace ratio whereas blueberry pomace requires a 10:1 water to pomace ratio. The amount of water necessary to prepare the pomace for freeze shearing is easily determined by following the process outlined below in the examples. Essentially, sufficient water is added to form and maintain a vortex in a Vitamix or other suitable blender for about 5 minutes. When frozen, such hydrated pomace provides an ideal substrate for freeze shearing.


The mixture is then frozen at 0 to −20° C., for example −9° C., until suitably frozen, typically 12-20 hours. The frozen puree is then subjected to at least one cycle of shearing such as with the Pacojet shearing process. The result is a smooth frozen puree which, when eaten, exhibits little or no grittiness experienced with the untreated puree/slurry.


Upon thawing at room temperature, a smooth, thick puree with the consistency of dairy pudding can be obtained. The continuous hydration and shear of the pomace increases viscosity and film forming ability. The unexpected result indicates shear and hydration can be used to slightly modify fruit fiber to create a useful food ingredient.


Before or after size reduction, components that provide off-tastes may be removed from the fruit or vegetable intermediate product or from the pomace. The components (e.g. bitter nutrients such as certain phytonutrients for example) may be left in the fruits and vegetables or pomace, or may be removed before or after particle size reduction through steam-stripping or blenching, or some type of filtration such as micro, ultra, nano filtration or reverse osmosis and then replaced (e.g. after encapsulation via a typical shell and core or even replaced through an emulsion system or some other process to overcome the off-taste). Steam stripping or blenching can also eliminate undesirable enzymatic reactions including enzymatic browning, and also initial load of microorganisms.


If pH adjustment had not been made prior in the process (before particle size reduction) then sufficient amounts of organic acids such as citric acid, lactic acid, acetic acid, malic acid, succinic acid, or juices such as lemon juice and/or acidic juice concentrate may be added to the size-reduced product to reduce the pH as desired.


The size-reduced fruit or vegetable or pomace product may then be used to prepare a beverage or food product. If necessary, the micro-ground product may be subjected to partial dehydration to remove some of the moisture. The resulting products are combined with base juices to form a beverage formulation which optionally may be homogenized and then pasteurized.


The size-reduced fruit or vegetable or pomace product may be used as main ingredients of fruit and vegetable food products or may be included in various food products to provide enhanced nutrition and other characteristics, such as color, flavor, and mouthfeel. Suitable food products include, but are not limited to beverages, soups, spreads, puddings, smoothies, snack foods, yogurts, and cereals.


The size-reduced fruit or vegetable or pomace product may be added to products that usually contain little or no real fruit, such as gummy snacks that contain less than 2% fruit, or multiphase snacks with a jam filling that is mostly non-fruit ingredients such as fillings for toaster tarts and the like. The size-reduced fruit or vegetable or pomace product may be used as a substitute for a combination of fruit juice or juice concentrate with a thickener (gum) because it can provide both fruit benefits and viscosity.


The size-reduced fruit or vegetable or pomace product may be added to various types of beverages such as fruit and or vegetable juices, fruit smoothies, fruit beverages and fruit cocktails. This will enhance natural fiber and phytochemical contents and increase viscosity, smoothness and mouth filling.


The following table demonstrates the benefit of added pomace (or goo) to various base juices.
















Enhanced

pH of final


Base-Juice
Nutrients
Pomace/Goo
product







100% Orange
2.5 g
Orange Pomace
3.8



fiber/serving
 38 μm



Orange
100% Vitamin
Carrot Goo
3.8


Carrot
A/serving




Orange
2.5 g
Carrot Pomace
3.8


Carrot
fiber/serving +
125 μm (acidified




100% Vitamin
w/Lemon Juice




A/serving
conc.)



Red/Apple
2.5 g
Cranberry Goo
3.5



fiber/serving




Red/Apple
2.5 g
Cranberry Skin
3.4



fiber/serving
125 μm



Red/Apple
2.5 g
Cranberry Skin
3.0



fiber/serving
 38 μm









As shown in the following table, pomace ingredients (without treatment) are generally higher molecular weight and less soluble and dispersible than FIBERSOL-2 a modified corn starch by ADM or other common sources of fiber. However, treating pomace as described herein to reduce the particle size provides pomace having increased solubility and greater nutritional value.


















Soluble





Chemical
to
Total



Name
Composition
Insoluble
Fiber
Other nutrients







Fibersol
Resistant
2:1
 100%




Maltodextrin






α and bglycoside






linkage





Inulin
β-(2-1) fructosyl-
Soluble
 100%




fructose links





Pectin from
α-(1-4)-linked D-
Soluble
 100%



Citrus
galacturonicacid





β-Glucan
D-glucose linked by
Short:
75.1%




β-glycosidicbonds
soluble
(oat)





Long:






insoluble




Cranberry
Mainly β -(1-4)
1:10
72.4%
Proanthocyanins


Pomace
glucoside





Cranberry
Mainly β -(1-4)
1:5
  43%
Anthocyanins


Goo
glucoside





Carrot
β -(1-4) glucoside,
1:1
52.8%
Carotenoids


Pomace
α -(1-4) galacturonic


Minerals



acid





Orange
α -(1-4) galacturonic
1:2
  54%
Carotenoids,


Pomace
acid, β -(1-4)


saponins,



glucoside


limonin









Dietary fiber comprises the remnants of edible plants cells, polysaccharides, lignin, and associate substances (carbohydrates) resistant to (hydrolysis) digestion by alimentary enzymes of humans.


AOAC fiber analysis is used to detect the amount of fiber in a food ingredient. This is important so that the amount of dietary fiber may be accurately reported in an ingredient/nutritional content disclosure. In another aspect of the invention, the pomace is pre-heated to inactivate natural enzymes present in the fruits or vegetables ingredients present in the pomace. The pomace is pre-heated to a temperature of at least about 70° C., to about 100° C. The pre-heating occurs prior to AOAC fiber analysis to maximize the fiber detection. It was discovered that such pre-heating treatment results in increasing the measured fiber content of the material and modifying the structure of the fiber. The heating may be done prior to particle size reduction, afterwards, both, or neither.


Example 1

Orange juice (200 g), carrot pomace treated to reduce the particle size (43 g), lemon Juice (2 g), and carrot goo (1 g) were combined to form a juice product. The resulting juice product had the following nutritional characteristics based on an 8 oz serving. Calories: 100; Total Fat: 0 g; Cholesterol 0 mg; Total Carb 25 g (Dietary Fiber 3 g; Sugars 20 g); Vitamin A: 220%; Vitamin C: 100%.


Example 2

Wet orange pomace was used in various forms to compare measured fiber. AOAC method 991.43; All results are listed in dry basis.



















Total Fiber
Soluble
Insoluble



Sample
(%)
Fiber (%)
Fiber (%)





















Wet Pomace






Raw
15
6.9
7.7



Micro-Ground Pomace






125 micron
27
12.1
14.7



 75 micron
28
12.5
15.7



 38 micron
37
21.7
15.1



Dried Pomace






Heated + Freeze Dried
54
35.5
18.4










Example 3

Two samples of orange pomace were subjected to AOAC analysis. Oranges were peeled and then juiced in an electric juicer. The resulting pomace was washed with water. The first sample (control) had no heat treatment. The second sample was heated for 5 minutes in boiling water and then drained. Both samples were freeze dried. Fiber and pectin analysis was conducted on the sample solids.

















TDF
Soluble Fiber
Insoluble Fiber
Pectin



(%)
(%)
(%)
(mg/100 g)







Control
33
11.4
21.3
3244


Heat-Treated
46
15.7
30.0
4574









It was concluded that inactivation of naturally existing enzymes (including pectinase) by heating increases fiber content.


Example 4

Four gallons of cranberry fine pomace were mixed with cranberry juice. One part of this material was mixed with four parts concentrated juice and water (16 gallons). The resulting slurry was subjected to three steps of reduction. Step one used the 212084-1 microcut head for 125 micron product which step produced about 4″ of “foam” on top of the cranberry liquid. The result indicated a good reduction of fine cranberry pulp with cranberry “skins”.


Step two further processed the step one product into a 21608-1 microcut head for 75 micron product. Not much foam was produced in step two. The result indicated a good further reduction of cranberry pulp was accomplished in this step.


Step three further processed the step two product through a 216084 microcut head for 35 micron product. Almost no foam was produced in the last step. The result indicated a good final reduction of cranberry pulp in cranberry juice/water.


Example 5

Fruit and vegetable pomace was reduced to as small as possible without having to add extra juice. Orange pulp was cut into 2″ pieces so that the pieces could be fed into a cutting head for further reduction. There was a very good reduction of the 2″ sized cubes of orange pulp into free flow 0.090″ pieces of orange pulp. This material fed easily into a microcut impeller having a 212084-1 microcut head. The resulting reduced orange juice pulp mixed well with water.


The carrot pulp was too thick as received. One part carrot juice (by weight) was added to one part carrot pulp and mixed well so that the material could go into the 216084-1 microcut head 35 micon. Moisture content (M.C.) 87.15%. There was a very good further reduction of carrot slurry into a fine puree which floated in water.


Example 6

Cranberry pulp (skin and seeds) was mixed with 1 part cranberry juice to 1 part pulp and mixed well. The M.C. was 55.11%. The 1:1 cranberry pulp slurry was reduced in the 200084-2° microcut head and produced a dry ribbon of pulp. This ribbon extrusion is due to the cranberry seeds being reduced. More liquid or juice was added to this product to go into the next reduction step. Three parts of juice was added to make the input material 3:1 or 62.9% M.C.


The second step of reduction on the 3:1 cranberry slurry was successful. An additional trickle of water was needed to avoid a pause in the feeding of the cranberry pulp slurry which would result in high temperature in the microcut cutting zone and steam being released from the material being cut.


Because the material that goes into the 216084 microcut head should be pourable, water was added to the cranberry pulp slurry to make it a 5:1 slurry with 76.66% M.C. The 5 parts liquid to 1 part cranberry pulp was sufficient to reduce the cranberry pulp to as fine as could be produced. Some of the pulp floated and some sank to the bottom of the container.


Example 7

A fine particle size fiber by dry grinding was created that reduces negative texture in foods and beverages. About 200 grams each of cranberry and blueberry pomace were ground for about 5 minutes in a “Vitamix” brand high shear blender. Next, the materials were sieved using a ROTAP machine to create about 50 grams of material that passed through a US 120 mesh sieve.


The dry grinding and sieve produced sufficient powder to pass through a 120 mesh powder. The powder had a greatly reduced gritty feel when rubbed between fingers.


Example 8

The ability of a “freeze-shear” process to reduce the particle size and functional properties of cranberry and blueberry pomace was evaluated. In the culinary world, a process sometimes called “Paco-Tizing” is used be chefs to create smooth food purees. The process involves cooking of various foods, adding liquid to the food mixture, freezing the mixture, then subjecting to the “Paco-tizing” treatment. A blade is used to mechanically shave off thin shavings of ice from the frozen food—water matrix. The result is often a smooth purees than can be used for various culinary applications. The consistency of the resultant purees is often referred to as “baby-food”


It was discovered that applying this freeze-shear/shave process to a high fiber material creates a modified fiber with improved water sorbtion, texture, and food ingredient useful properties.


Fifty (50) grams each of cranberry pomace (Cran Chile) or blueberry pomace (Givaudan) was hydrated continuously with water in order to create a slurry or paste that could be frozen.


Try #1: 50 g of cold water was added to 50 g of cranberry pomace and mixed in a bowl (stainless steel) with a standard rubber spatula. The pomace immediately absorbed the water fully but did not produce a slurry. Water was added at 50 g increments to understand how much water must be added to make a slurry.













Cold H20



to Pomace
Observation







0:1
Dry pomace~70 mesh. Gritty and fibrous.


1:1
Water absorbs rapidly, pomace does not feel wet.


2:1
Pomace begins to swell but still dry to touch.


3:1
Crumb topping consistency.


4:1
Still crumbly.


5:1
Mush just begins to form, still too thick for a blender.



Transfer to Vitamix.


7:1
Mixture thick slurry but Vitamix blender cannot



continuously mix (no vortex)


8:1
As more water is added mixture THICKENS → indicates



fiber swelling and moisture sorbtion.


10:1 
Mixture still thickening Vitamix still cannot create a vortex.


15:1 
Same as 10:1 except the vortex is created intermittently.


20:1 
Vortex created and continuously sheared for 5 minutes.



Mixture appears to thicken somewhat but stabilize after 2-3



minutes of continuous shear.









Try #2: Hot water (boiling) was used with the same procedure as in try #1 with virtually the same result.


Try #3a: 50 g Blueberry pomace and cold water and required only 10:1 water:pomace to achieve desired consistency.


Try #3b: A batch for freeze-shearing was prepared with a 20:1 water cranberry pomace ratio. In particular, 50 g cranberry pomace added to 500 g cold (20° C.) water in a Vitamix blender (a 10:1 ratio.) Blending on high, a vortex/sustained shearing could not be attained. An additional 100 g cold water (12:1) was added with still no vortex. Another 100 g cold water (14:1) was added and a vortex started and stopped erratically. An additional 100 g cold water (16:1) as added and a vortex began but after a minute, it ceased as viscosity of the blending appeared to increase. A further 100 g cold water (18:1) was added with the same result as the 16:1 ratio—a vortex was created for about 1-1½ minute, but then stopped and the viscosity of the mixture appeared to increase again. A final 100 g cold water (20:1) was added whereby a vortex was created and stayed for 5 minutes on high. After 5 minutes, mixture began to “dry pop” or “splash” as viscosity of mixture still continued to increase. Mixing was stopped.


A small amount of the mixture was spread on a culinary roule rubber mat and dried in a low oven (about 170° F.). After an hour, it was apparent that a film of fiber was forming indicating the fiber was modified enough by the Vitamix process to form a cohesive matrix. Unsheared material did not form a film.


The remaining balance of the mixture was frozen in 1 L stainless steel canisters (for later freeze-shearing) at 16° F. The next day, ˜16 hour later, the frozen puree was subject to two cycles of the Paoject shearing process. The result was immediately apparent: a smooth frozen puree resulted. When eaten, the puree exhibited little or no grittiness experienced with the untreated puree/slurry.


Upon thawing at room temperature, a smooth, thick puree with the consistency of dairy pudding was obtained. The pudding had only a slight degree of grittiness on the tongue and a slightly acidic/tart and astringent after taste (due to the acids and polyphenol compounds contained in cranberry pomace).


Continuous hydration and shear of cranberry and blueberry pomace increased viscosity and created film forming ability. Around 20 parts water to 1 part cranberry pomace or 10 parts water to 1 part blueberry pomace was needed to create a fluid that could be continuously sheared in a high shear Vitamix mixer/blender. The unexpected result indicated shear and hydration can be used to slightly modify fruit fiber to create a potentially useful food ingredient. Subsequent “freeze-shearing” created a relatively smooth pudding like product. The Vitamix or freeze-shear process alone did not have the same effect as the combination of the two treatments.


Example 9

Samples Prepared:


A. Naked Juice blue machine fiber enrichment. Blended 15.2 g blueberry pomace that had been freeze-sheared after Vitamix hydration and sheer 1/6=n % s and contained 5 parts H2O 1 part pomace (12% total fiber based on calculation), 186.4 g Naked Juice Blue machine smoothie, and 38.4 g tap water. Thus 15.2 g×0.12 or 1.8 g fiber from pomace blend was prepared by shaking 10 seconds in a Naked Juice bottle. The resulting beverage had scant to no gritty texture.


B. Naked juice blue machine with cranberry pomace blend.








150





g





20


:






1





cranberry






pomace


(

=



1





g





pomace


21





g





sturry


×
150
×


72





g





fiber


g





pomace




)



=

5





g





fiber


,





25 g sucrose (granulated), 0.34 g citric acid, and 0.16 g malic acid. Resulting blend had low to no gritty texture, low sweetness, and high tartness. Illustrated ability to add excellent source of fiber to a beverage.


C. Formula A above was prepared with 2× blueberry pomace. The blend provided a good source of fiber and some gritty texture.


D. Blended 6 oz of Yoplait blueberry yogurt with 21 g 5:1 blueberry pomace to obtain approximately a good source (2.5 g fiber/serving) fiber. Nice and smooth texture, no grit although flavor balance lost when added to commercial product.


E. Cranberry-fiber “pudding”/thick smoothie as prepared by combining 116 g (20:1) cranberry pomace “pudding,” 33 g sucrose, 0.5 g citric acid, 0.3 g malic acid, and 0.2 aspartame. The resulting smoothie had minimal grittiness. The acid and sweetener were balanced for more optimal taste.


While the invention has been described with respect to specific examples including presently preferred modes of carrying out the invention, those skilled in the art will appreciate that there are numerous variations and permutations of the above described systems and techniques that fall within the spirit and scope of the invention as set forth in the appended claims.

Claims
  • 1. A method of processing pomace comprising obtaining a pomace press cake by extracting juice from a fruit, vegetable, or combinations thereof;hydrating the pomace press cake;acidifying the pomace press cake with an organic acid;micro-grinding the hydrated, acidified pomace press cake to reduce the particle size of the pomace to less than 250 microns.
  • 2. The method of claim 1 comprising reducing the particles size to less than 125 microns.
  • 3. The method of claim 1 comprising reducing the particles size to less than 38 to 125 microns.
  • 4. The method of claim 1 further comprising heating the pomace press cake to at least 70° C. before, during, or after reducing the particle size.
  • 5. The method of claim 1 further comprising heating the pomace press cake with heat generated by the friction of grinding blades during size reduction.
  • 6. The method of claim 1 further comprising acidifying the pomace press cake with citric acid, lactic acid, acetic acid, malic acid, or succinic acid.
  • 7. The method of claim 1 comprising freezing the hydrated pomace cake at a temperature of 0 to −20° C. prior to reducing the particle size.
  • 8. The method of claim 1 further comprising treating the pomace press cake to remove undesired components therefrom.
  • 9. The method of claim 1 further comprising, after micro-grinding the pomace press cake, homogenizing the micro-ground pomace.
  • 10. The method of claim 1 wherein the pomace press cake is obtained from juice extraction from a fruit or vegetable selected from the group consisting of carrot, cranberry, orange, blueberry, tomato, apple, lemons, limes, grapes, strawberries, grapefruits, tangerine, mandarin orange, tangelo, pomelo, celery, beet, lettuce, spinach, cabbage, artichoke, broccoli, brussels sprouts, cauliflower, watercress, peas, beans, lentils, asparagus, onions, leeks, kohlrabi, radish, turnip, rutabaga, rhubarb, carrot, cucumber, zucchini, eggplant, pineapple, peach, banana, pear, guava, apricot, watermelon, Saskatoon berry, blueberry, plains berry, prairie berry, mulberry, elderberry, Barbados cherry (acerola cherry), choke cherry, date, coconut, olive, raspberry, strawberry, huckleberry, loganberry, currant, dewberry, boysenberry, kiwi, cherry, blackberry, quince, buckthorn, passion fruit, rowan, gooseberry, pomegranate, persimmon, mango, papaya, lychee, plum, prune, fig, and any combination thereof.
  • 11. The method of claim 1 wherein the pomace press cake is obtained from juice extraction from a fruit or vegetable selected from the group consisting of carrot pomace, orange pomace, cranberry pomace, or mixtures thereof.
  • 12. The method of claim 1 further comprising testing fiber content of the pomace comprising heating the pomace up to 100° C. for a time sufficient for enzyme inactivation and then subjecting the pomace to AOAC analysis.
  • 13. The method of claim 1, wherein the hydrating step further comprising: adding water to the pomace press cake.
  • 14. The method of claim 13, further comprising: adding sufficient water to the pomace press cake to maintain a vortex in a blender.
Parent Case Info

The application claims priority to U.S. provisional application Ser. No. 61/390,720 filed Oct. 7, 2010, hereby incorporated by reference in its entirety.

US Referenced Citations (246)
Number Name Date Kind
2595181 Vincent Apr 1952 A
2596662 Ducker May 1952 A
3037975 Cohn Jun 1962 A
3118770 Harrell Jan 1964 A
3404012 Craig Oct 1968 A
3860734 Huth Jan 1975 A
3959518 Vincent May 1976 A
3966984 Cooke Jun 1976 A
3973051 Buckley Aug 1976 A
4016351 Eschinasi Apr 1977 A
4113573 Gerow Sep 1978 A
4241093 Farag Dec 1980 A
4259252 Perry Mar 1981 A
4304768 Staub Dec 1981 A
4313372 Gerow Feb 1982 A
4377520 Howell Mar 1983 A
4413017 Loader Nov 1983 A
4488912 Milch Dec 1984 A
4497838 Bonnell Feb 1985 A
4503079 King Mar 1985 A
4525172 Eriksson Jun 1985 A
4526794 Altomare Jul 1985 A
4565702 Morley Jan 1986 A
4587126 Patton May 1986 A
4644905 Vierling Feb 1987 A
4690827 Kupper Sep 1987 A
4747881 Shaw May 1988 A
4774099 Feeney Sep 1988 A
4810517 Glittenberg Mar 1989 A
4830862 Braun May 1989 A
4842877 Tyson Jun 1989 A
4865863 Prosise Sep 1989 A
4871569 Anderson Oct 1989 A
4876102 Feeney Oct 1989 A
4895729 Powrie et al. Jan 1990 A
4925686 Kastin May 1990 A
4952504 Pavilon Aug 1990 A
4959227 Amer Sep 1990 A
4971823 Fahlen Nov 1990 A
4988530 Hoersten Jan 1991 A
5007334 Kobes Apr 1991 A
5008254 Weibel Apr 1991 A
5024996 Ringe Jun 1991 A
5034378 Cox Jul 1991 A
5073397 Tarr Dec 1991 A
5099009 Thibault Mar 1992 A
5106634 Thacker et al. Apr 1992 A
5137744 Cagley Aug 1992 A
5162128 Mills Nov 1992 A
5196222 Kirk Mar 1993 A
5202122 Graves Apr 1993 A
5232726 Clark Aug 1993 A
5234704 Devine Aug 1993 A
5260086 Downton et al. Nov 1993 A
5312636 Myllymaki et al. May 1994 A
5385748 Bunger Jan 1995 A
5403612 Huang Apr 1995 A
5472952 Smidt Dec 1995 A
5474793 Meyer Dec 1995 A
5480788 Devic Jan 1996 A
5567424 Hastings Oct 1996 A
5567462 Ehrlich Oct 1996 A
5612074 Leach Mar 1997 A
5616355 Haast Apr 1997 A
5627269 Herak May 1997 A
5639494 Grassin Jun 1997 A
5656310 Santillo, Jr. Aug 1997 A
5690981 Watanabe Nov 1997 A
5738887 Wu Apr 1998 A
5759704 Horiuchi Jun 1998 A
5766662 Inglett Jun 1998 A
5773075 Todd Jun 1998 A
5817381 Chen Oct 1998 A
5837311 Zelkha et al. Nov 1998 A
5861178 Burgin Jan 1999 A
5927187 Bosch Jul 1999 A
5932265 Morgan Aug 1999 A
5958474 Lee Sep 1999 A
5964983 Dinand Oct 1999 A
6019851 Pittet Feb 2000 A
6020016 Castleberry Feb 2000 A
6022580 Akatsuka Feb 2000 A
6083582 Chen Jul 2000 A
6151799 Jones Nov 2000 A
6183806 Ficca et al. Feb 2001 B1
6361818 Biyani Mar 2002 B2
6383546 Powrie May 2002 B1
6506435 Lundberg Jan 2003 B1
6523496 Keithly Feb 2003 B1
6645546 Roney Nov 2003 B2
6730343 Chung May 2004 B2
6753019 Lang Jun 2004 B1
6787172 McArdle et al. Sep 2004 B2
6787372 McArdle et al. Sep 2004 B1
6830771 Lanter Dec 2004 B2
6841181 Jager Jan 2005 B2
6890578 Takahasi May 2005 B1
7052725 Chang May 2006 B2
7060313 Jones Jun 2006 B2
7074300 Lundberg Jul 2006 B2
7094317 Lundberg Aug 2006 B2
7485332 Chu Feb 2009 B2
7560132 Newkirk Jul 2009 B2
7563471 Keithly Jul 2009 B2
7576070 Kunz Aug 2009 B2
7582213 Lundberg Sep 2009 B2
7629010 Passarelli Dec 2009 B2
7629011 Suasin Dec 2009 B2
7833558 Larsen Nov 2010 B2
7879379 Widmer Feb 2011 B1
7887862 Paz Briz Feb 2011 B2
7910338 Hennessey Mar 2011 B2
8017171 Sample Sep 2011 B2
8021520 Yao Sep 2011 B2
8034286 Janssen Oct 2011 B2
8481099 Nafisi-Movaghar Jul 2013 B2
8673382 Jordan Mar 2014 B2
20010012525 Mann Aug 2001 A1
20010016220 Jager Aug 2001 A1
20010046550 Wadsworth Nov 2001 A1
20010053404 William Dec 2001 A1
20020034563 Grassin Mar 2002 A1
20020054924 Leahy May 2002 A1
20020127319 Gare Sep 2002 A1
20030040489 Tanaka Feb 2003 A1
20030049360 Zietlow Mar 2003 A1
20030064140 Lineback Apr 2003 A1
20030068357 Vala Apr 2003 A1
20030068429 Frippiat Apr 2003 A1
20030144245 Addis Jul 2003 A1
20030194473 Redding Oct 2003 A1
20040022877 Green Feb 2004 A1
20040081734 Lang Apr 2004 A1
20040081741 Levi Apr 2004 A1
20040086626 Lundberg May 2004 A1
20040126474 Letourneau Jul 2004 A1
20040170731 Subramaniam Sep 2004 A1
20040213886 Toves Oct 2004 A1
20040265451 Rooks et al. Dec 2004 A1
20040265465 Daniels Dec 2004 A1
20050058763 Cetrulo Mar 2005 A1
20050074542 Lundberg Apr 2005 A1
20050089614 Jones Apr 2005 A1
20050089620 Bialek Apr 2005 A1
20050106308 Rudie et al. May 2005 A1
20050112242 Hessel May 2005 A1
20050118233 Segal Jun 2005 A1
20050118326 Anfinsen Jun 2005 A1
20050158541 Tanaka Jul 2005 A1
20050169970 Aquino Aug 2005 A1
20050175672 Kleutz Aug 2005 A1
20050202149 McClements Sep 2005 A1
20050271790 Aronson Dec 2005 A1
20050274469 Lundberg Dec 2005 A1
20060062862 Haber Mar 2006 A1
20060093720 Tatz May 2006 A1
20060099277 Jewett, Jr. May 2006 A1
20060115564 Passarelli Jun 2006 A1
20060180547 Widmer Aug 2006 A1
20060182820 Kluetz Aug 2006 A1
20060188621 Jones Aug 2006 A1
20060204624 Patist Sep 2006 A1
20060210673 Petrofsky Sep 2006 A1
20060210687 Lundberg Sep 2006 A1
20060216397 Kerkman Sep 2006 A1
20060240077 Hansen Oct 2006 A1
20060251789 Lundberg Nov 2006 A1
20060263416 Brent Nov 2006 A1
20060263487 Brent Nov 2006 A1
20060286260 Nayak Dec 2006 A1
20070031572 Larsen Feb 2007 A1
20070082026 Aimutis Apr 2007 A1
20070082027 Aimutis Apr 2007 A1
20070082028 Aimutis Apr 2007 A1
20070082029 Aimutis Apr 2007 A1
20070082030 Aimutis Apr 2007 A1
20070082085 Catani Apr 2007 A1
20070087084 Coleman Apr 2007 A1
20070110684 Jensen May 2007 A1
20070110875 Keithly May 2007 A1
20070116837 Prakash May 2007 A1
20070202211 Altom Aug 2007 A1
20070298078 Harrison Dec 2007 A1
20080014303 Jacops Jan 2008 A1
20080020094 Lager Jan 2008 A1
20080032015 Walpole Feb 2008 A1
20080113079 Takamine May 2008 A1
20080166464 Lundberg Jul 2008 A1
20080193590 Lundberg Aug 2008 A1
20080233238 Roney Sep 2008 A1
20080248185 Ferrari Oct 2008 A1
20080305096 Verdegem Dec 2008 A1
20080311174 Sakamoto Dec 2008 A1
20080311265 MacDonald Dec 2008 A1
20080311272 Wild Dec 2008 A1
20090011111 Sugiura Jan 2009 A1
20090022877 Sample Jan 2009 A1
20090035441 Hirashima et al. Feb 2009 A1
20090110798 Gusek Apr 2009 A1
20090123597 Williams May 2009 A1
20090130291 Driggers May 2009 A1
20090169680 Rabault Jul 2009 A1
20090186383 Mancosky Jul 2009 A1
20090215704 Johnson Aug 2009 A1
20090220665 Vanhemelrijck Sep 2009 A1
20090258111 Takayanagi Oct 2009 A1
20090269376 Lundberg Oct 2009 A1
20090274811 Lundberg Nov 2009 A1
20090297671 Basker Dec 2009 A1
20090297682 Dukan Dec 2009 A1
20100015302 Bates Jan 2010 A1
20100040728 Henderson Feb 2010 A1
20100086511 Sakamoto Apr 2010 A1
20100092620 Bravo Apr 2010 A1
20100099648 Debon Apr 2010 A1
20100159100 Buysschaert Jun 2010 A1
20100166913 Stewart Jul 2010 A1
20100233342 Almeida Sep 2010 A1
20100234569 Helling Sep 2010 A1
20100248320 Lyons Sep 2010 A1
20110020525 Homsma Jan 2011 A1
20110081336 Medoff Apr 2011 A1
20110086133 Puranen Apr 2011 A1
20110129591 Jordan Jun 2011 A1
20110151056 Chukwu Jun 2011 A1
20110223294 Itoh Sep 2011 A1
20110250314 Lager Oct 2011 A1
20110268860 Lundberg Nov 2011 A1
20110278153 Bates Nov 2011 A1
20110287151 Simunovic Nov 2011 A1
20110293814 Alexandre Dec 2011 A1
20110305808 Sample Dec 2011 A1
20110308141 Christensen Dec 2011 A1
20110311599 Boursier Dec 2011 A1
20110311708 Wang Dec 2011 A1
20120016026 Bromley Jan 2012 A1
20120037013 Bertocchi Feb 2012 A1
20120040068 Eveland Feb 2012 A1
20120040073 Fox Feb 2012 A1
20120088015 Han et al. Apr 2012 A1
20120135124 Letourneau May 2012 A1
20130064947 Nafisi-Movaghar Mar 2013 A1
20130123374 Gusek May 2013 A1
20140234476 Balasubramanian Aug 2014 A1
20140308389 Ames Oct 2014 A1
20160000130 Balasubramanian Jan 2016 A1
Foreign Referenced Citations (51)
Number Date Country
1909805 Feb 2007 CN
101669550 Mar 2010 CN
101715947 Jun 2010 CN
0061305 Sep 1982 EP
0485030 May 1992 EP
2624710 Aug 2013 EP
2712464 Nov 1993 FR
635053 Apr 1950 GB
1045243 Oct 1966 GB
1341963 Dec 1973 GB
5898055 Jun 1983 JP
3146593 Jun 1991 JP
H06335371 Jun 1994 JP
10210956 Aug 1998 JP
2001128637 May 2001 JP
2004261039 Sep 2004 JP
20120010680 Feb 2012 KR
2202257 Apr 2003 RU
2342854 Jan 2009 RU
199427451 Dec 1994 WO
199903892 Jan 1999 WO
200005310 Feb 2000 WO
2000013532 Mar 2000 WO
2001032978 May 2001 WO
2001078859 Oct 2001 WO
2001084965 Nov 2001 WO
2004008881 Jan 2004 WO
2004044285 May 2004 WO
2004085484 Oct 2004 WO
2005087200 Sep 2005 WO
2006096884 Sep 2006 WO
2007092087 Aug 2007 WO
2007092448 Aug 2007 WO
2007139563 Dec 2007 WO
2008068572 Jun 2008 WO
2008077594 Jul 2008 WO
2008089821 Jul 2008 WO
2008095254 Aug 2008 WO
2008104779 Sep 2008 WO
2008112957 Sep 2008 WO
WO 2008104779 Sep 2008 WO
2008134626 Nov 2008 WO
2009013395 Jan 2009 WO
2010010219 Jan 2010 WO
2010084240 Jul 2010 WO
2010093864 Aug 2010 WO
2011024183 Mar 2011 WO
2012016201 Feb 2012 WO
2012045045 Apr 2012 WO
2012047786 Apr 2012 WO
2015146880 Jan 2015 WO
Non-Patent Literature Citations (40)
Entry
PCT/US2011/054556, International Search Report and Written Opinion, dated Feb. 6, 2012.
Oct. 16, 2014—(CA) Office Action—App 2814059.
Bagherian et al., “Comparisons Between Conventional Microwave- and Ultrasound-Assisted Methods for Extraction of Pectin From Grapefruit,” Chemical Engineering and Processing, vol. 50, 2011, pp. 1237-1243.
Bourquin, L.D., et al., Vegetable fiber fermentation by human fecal bacteria: Cell wall polysaccharide disappearance and short-chain fatty acid production during in vitro fermentation and water-holding capacity of unfermented residues. Journal of Nutr. 123: 860-869. (Abstract), 1993.
Corey et al. “Witloof Chicory: A New Vegetable Crop in the United States” 1990 Advances in New Crops Timber Press pp. 414-418 Accessed from http://www.hort.purdue.edu/newcrop/proceedings1990/v1-414.html pp. 1-8.
Dongowski, G., et al., The degree of methylation influences the degradation of pectin in the intestinal tract of rats and in vitro. J. Nutr. 132: 1935-1944. (Abstract), 2002.
Endress, Hans Ulrich, et al, In Fiber ingredients: Food applications and health benefits. Susan Sung Cho and Priscilla Samuels, eds. CRC Press Francis Taylor Group, Boca Raton FL. (Summary), 2009.
Fishman, M.L., et al., Component and global average radii of gyration of pectins from various sources. Carbohydrate Polymers 15: 89-104, 1991.
Gibson, G.R., et al., Dietary modulation of the colonic microbiota: Introducing the concept of prebiotics. J. Nutr. 125: 1401-1412, 1995.
Gibson, G.R., et al., “Handbook of Prebiotics” CRC Press Taylor & Francis Group. Boca Raton, FL. (Table of Contents), 2008.
Grootaert, C., et al., Microbial metabolism and prebiotic potentcy of arabinoxylan oligosaccharides in the human intestine. Trends in Food Science & Technology. 18(2): 64-71. (Abstract), 2007.
Gulfi, M., et al, Influence of structure on in vitro fermentability of commerical pectins and partially hydrolysed pectin preparations. Carbohydrate Polymers 59: 247-255, 2005.
Gulfi, M., et al., The Chemical characteristics of apple pectin influence its fermentability in vitro. LWT 39: 1001-1004, 2006.
Gulfi, M., et al., In vitro fermentability of a pectin fraction rich in hairy regions. Carbohydrate Polymers, 67: 410-416, 2007.
Hellin et al., “Changes in High and Low Molecular Weight Carbohydrates During Rhizopus Nigricans Cultivation on Lemon Peel,” Carbohydrate Polymers, vol. 45, 2001, pp. 169-174.
Hotchkiss, A.,et al., New bioactive and biobased product applications of pectin. In Pectins and Pectinases, Schols et al. editors. Wageningen Academic Publisher, Wageningen, The Netherlands (Summary and Abstract), 2009.
International Search Report and Written Opinion in PCT/US10/57778, dated Mar. 2, 2011.
International Search Report and Written Opinion in PCT/US15/14850 dated Jun. 25, 2015.
International Search Report and Written Opinion in PCT/US11/62303 dated Apr. 5, 2012.
International Search Report and Written Opinion in PCT/US14/15326 dated May 9, 2014.
“Isolate” Merriam-Webster.com. Merriam-Webster, n.d. Web., Mar. 27, 2014. http://www.merriam-webster.com/dictionary/isolate 2 pages.
Khan, M.K. et al, Ultrasound-assisted extraction of polyphenols (flavanone glycosides from orange (Citrus sinensis L.) peel. Food Chemistry, 119, 851-858. (Abstract).
Manderson, K., et al, In vitro determination of prebiotic properties of oligosaccharides derived from an orange juice manufactoring by-product stream. Applied and environmental microbology. 71(12):8383-8389, 2005.
Nicolini, L., Changes in in-vitro digestibillity of orange peels and distillery grape stalks after solid-state fermentation by higher fungi. Bioresource Technology 45(1): 17-20 (Abstract), 1993.
Olano-Martin, E., et al, Comparison of the in vitro bifidogenic properties of pectins and pectic-oligosaccharides. J Appl Microbiol 93: 505-511, 2002.
Roth, J.A., et al, Pectin improves colonic function in rat short bowel syndrome. Journal of surgical research 58: 240-246. (Abstract), 1995.
Salyers, A. A., et al, Fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon. Applied and Environmental Microbology. 1977: 529-533 (Abstract).
Sunvold, G.D., et al., In vitro fermentation of cellulose, beet pulp, citrus pulp, and citrus pectin using fecal inoculum from cats, dogs, horses, humans, and pigs and ruminal fluid from cattle. J Anim Sci 1995. 73:3639-3648, 1995.
Titgemeyer, E.C., et al, Fermentability of various fiber sources by human fecal bacteria in vitro. Am J of Clinical Nutr 53:1418-1424. (Abstract), 1991.
Tripodo, M.M., et al, Citrus waste recovery: a new environmentally friendly procedure to obtain animal feed. Bioresource Technology 91(2): 111-115. (Abstract), 2004.
“Understanding Mesh Sizes and Microns”, www.skylighter.com/fireworks/making-fireworks-projects/screen-mess-metal-particl . . . May 31, 2013. , p. 1.
Van Den Broek, L.A.M., Voragen, A.G.J., 2008. Bifidobacterium glycoside hydrolases and (potential) prebiotics. 2008. Innovative Food Science & Emerging Technologies 9: 401-407. (Abstract and Industrial Relevance).
Veldius, M.K., et al., Oil- and water-soluble aromatics distilled from citrus fruit and processing waste J. of Food Science 37: 108-112. (p. 108), 1972.
Will, et al., “Processing and Analytical Characterisation of Pulp-Enriched Cloudy Apple Juices,” Elsevier, LWT—Food Science and Technology, vol. 41, No. 10, 2008, pp. 2057-2063.
Yamada, H., et al, Recent studies on structures and intestinal immunity modulating activities of pectins and pectic polysaccharides from medicinal herbs. In Pectins and Pectinases, Schols et al. editors. Wageningnen Academic Publisher, Wageningen, The Netherlands. (Abstract), 2009.
Hayat, Khizar, “Effect of microwave treatment on phenolic content and antioxidant activity of citrus mandarin pomace,” Food Chemistry 123 (2010) 423-429, XP027086084, (7 pages).
Panquin: “Funcitional and Speciality Beverage Technology”; Elsevier, Jan. 29, 2009, Technology & Engineering—(512 pages).
Rockenbach, Ismael Ivan, “Phenolic compounds content and antioxidant activity in pomace from selected red gapes (Vitis vinifera L. and Vitis labrusca L.) widely produced in Brazil,” Food Chemistry 127 (2011) 174-179, XP028361805 (6 pages).
Stoll, T. et al. 2003. Food, Argic and Environ. 1: 88-92, Year 2003, (4 pages).
Stoll, T. et al. 2003. Inv Food Sci. Emg. Technol. 4:415-423, Year 2003, (9 pages).
Related Publications (1)
Number Date Country
20120088015 A1 Apr 2012 US
Provisional Applications (1)
Number Date Country
61390720 Oct 2010 US