The invention relates to processing and use of whole fruits and vegetables and to processing of side-stream ingredients of juice extraction and paste production. More particularly, the invention relates to methods of reducing the particle size of whole fruits and vegetables or side-stream ingredients thereof. The invention further relates to use of the processed whole fruits and vegetables and use of the processed side-stream ingredients in beverage and food products.
Whole vegetables and fruits are generally not used in beverages as they contain ingredients such as skins, seeds, stems, roots, and the like which may affect the taste and mouthfeel of the beverage. Instead, juices and/or pulp are obtained from the vegetables and fruits and the skins, seeds, stems, roots, and the like are discarded. These discarded ingredients are often in the form of pomace.
Pomace is the pulpy edible by-product remaining after fruit or vegetable juice pressing processes, wine crush operations, puree and concentrate operations, canning processes, and other food manufacturing processes. Pomace may include, for example, skins, pulp, seeds, and edible part of stems of the fruit and vegetable such as apples or carrots. In some cases the pomace can derive or contain other parts of the fruit and vegetable such as pod, stalk, flower, root, leaves and tuber. In a juice extraction process, the pomace is typically in the form of a part of press cake. By-products from paste and puree processes such as tomato skins and seeds from tomato ketchup and paste processing are also included in the pomace even they are not the by-products from juice extracts. Fruit skins from cannery are also edible by-products. Hereafter, pomace includes all edible by-products from fruit and vegetable juice, paste, puree and canning processes.
Pomace has been used for fertilizer and substrates for microorganism growth, for example, and dried pomace has had limited use in food products such as soups and snacks, and also has been used in the dietary supplement industry. However, pomace is generally not used in food products due to its gritty texture, sedimentation, fibrous nature, high insoluble fiber content, intense flavor and lack of starch and protein. Thus pomace is generally considered a waste by-product in the fruit and vegetable industry.
For example, cranberry pomace remains after the squeezing of juice for cranberry type cocktails and juices and concentrates. These cranberry pomace materials have been found to contain 70% to 75% fiber with in insoluble to soluble fiber ratio of 9 or 10 to 1 (wet basis) less than 5%-10% protein, and less that 5% sugars and starches. Thus, by-products are typically removed from the juicing process due to challenges with texture and flavor when creating a juice beverage.
It would be beneficial to process pomace as well as whole fruits and vegetables to arrive at a useful food ingredient in order to avoid such waste.
In one aspect, the invention relates to whole fruits and vegetables which have been processed to provide a product having particle and fiber size less than 250 microns and their use in beverage and food products.
In another aspect, the invention relates to a method of processing whole fruits and vegetables to provide a product having particle and fiber size less than 250 microns to improve texture and dispersion properties.
In another aspect, the invention relates to side-stream ingredients (pomace) having particle and fiber size less than 250 microns and its use in beverage and food products.
In another aspect, the invention relates to a method of reducing the particle and fiber particle size of pomace to less than 250 microns to improve texture and dispersion properties.
In another aspect, the invention relates to a method of reducing the particle and fiber size of pomace wherein the pomace is hydrated and frozen prior to micro-grinding.
In another aspect, the invention relates to pre-treating the whole fruits and vegetables or pomace with heat prior to AOAC fiber analysis to maximize fiber detection.
Side-stream ingredients (hereinafter “pomace”) generally contains high total dietary fiber content (50 or more percent by weight), low amounts of in sugars typically less than 5% but more commonly less than 2% by weight in wet pomace, and varying amounts of essential vitamins, minerals and phytonutrients (depending on types of fruit/vegetable and process applied). Likewise, whole fruits and vegetables also contain fiber and nutrient content much higher than the extracted juice products and thus are much healthier.
Whole Fruits and Vegetables
Whole vegetables and fruits include juices, pulp, skins, seeds, roots, and the like. Aspects of the invention utilize the entire vegetable or fruit to prepare a product for consumption either alone or in combination with other ingredients. In accordance with the present invention, the desired product has a reduced particle size of less than 250 microns. The whole fruits and vegetables are pre-treated prior to size reduction by, for example, crushing, cutting, pulverizing, or homogenizing to form an intermediate product. This intermediate product is then subjected to particle (including fiber) size reduction as discussed in more detail below.
Prior to particle size reduction, the intermediate product may be acidified to lower the pH. Typically the product is acidified to under pH 4.5 for microbial stability. Sufficient amounts of organic acids such as citric acid, lactic acid, acetic acid, malic acid, succinic acid, or juices such as lemon juice and/or acidic juice concentrate may be added to the product to reduce the pH of product to a desired pH.
Whole fruits and vegetables any be any suitable fruit or vegetable such as, but not limited to, carrot, cranberry, orange, blueberry, tomato, apple, and any combination thereof. Lemons, limes, grapes, strawberries, grapefruits, tangerine, mandarin orange, tangelo, pomelo, celery, beet, lettuce, spinach, cabbage, artichoke, broccoli, brussels sprouts, cauliflower, watercress, peas, beans, lentils, asparagus, onions, leeks, kohlrabi, radish, turnip, rutabaga, rhubarb, carrot, cucumber, zucchini, eggplant, pineapple, peach, banana, pear, guava, apricot, watermelon, Saskatoon berry, blueberry, plains berry, prairie berry, mulberry, elderberry, Barbados cherry (acerola cherry), choke cherry, date, coconut, olive, raspberry, strawberry, huckleberry, loganberry, currant, dewberry, boysenberry, kiwi, cherry, blackberry, quince, buckthorn, passion fruit, rowan, gooseberry, pomegranate, persimmon, mango, papaya, lychee, plum, prune, fig, or their combination.
Pomace (Side-Stream Ingredients)
Pomace a natural by-product from the extraction of juices from fruits and vegetables and other processes. Pomace contains natural nutrients (such as vitamin A, vitamin C, vitamin E, anthocyanins, and antioxidants), flavors, and/or colors of the original fruits and vegetables. Pomace also contains high natural (un-processed) fibers. Most pomace contains over 50% (d.b.) of dietary fiber, mostly insoluble fiber. The use of pomace in food products will fortify fiber and naturally existing nutrients such as vitamins and antioxidants. Hence the use of pomace provides an excellent natural and label-friendly way to enhance quality of food products including, for example, beverages, soups, spreads, puddings, smoothies and snacks. Moreover, pomace is generally a low cost ingredient because it is under-utilized and is considered a waste from juice industry.
Pomace may be obtained from any suitable fruit or vegetable such as, but not limited to, carrot, cranberry, orange, blueberry, tomato, apple, and any combination thereof. Lemons, limes, grapes, strawberries, grapefruits, tangerine, mandarin orange, tangelo, pomelo, celery, beet, lettuce, spinach, cabbage, artichoke, broccoli, brussels sprouts, cauliflower, watercress, peas, beans, lentils, asparagus, onions, leeks, kohlrabi, radish, turnip, rutabaga, rhubarb, carrot, cucumber, zucchini, eggplant, pineapple, peach, banana, pear, guava, apricot, watermelon, Saskatoon berry, blueberry, plains berry, prairie berry, mulberry, elderberry, Barbados cherry (acerola cherry), choke cherry, date, coconut, olive, raspberry, strawberry, huckleberry, loganberry, currant, dewberry, boysenberry, kiwi, cherry, blackberry, quince, buckthorn, passion fruit, rowan, gooseberry, pomegranate, persimmon, mango, papaya, lychee, plum, prune, fig, or their combination. In particular, pomace obtained from orange, cranberry, and carrot provides valuable nutrients and fiber.
Pomace may be obtained from any processing method that produces side-stream ingredients. For example pomace may be obtained and used as is from the press cake after a juice extraction process; however, such pomace generally has a wide range of particle and fiber sizes with a significant amount being large particles. For example, particle or fiber sizes of pomace may range from 100 microns to 5 cm depending upon the particular type of extracted fruit or vegetable. Pomace containing larger particles and fiber, and/or a wide distribution of particle or fiber sizes does not have a smooth texture. Therefore, mixing such pomace into beverage or snack formulations provides a tough, sandy, and gritty texture. Such pomace also causes a non-homogeneous dispersion in liquid-based products. For ease of discussion, the term “particle size” refers to both particle size and fiber size.
As used herein, pomace may also include the “goo” or the retentate formed from a filtration step of an extraction process, e.g., when clarifying a juice. This filtration retentate may be obtained as a by-product, for example, from any suitable fruit or vegetable juice such as carrot or cranberry juices. This retentate or goo may be added to pomace obtained from the press cake, for example, and is hereinafter is collectively referred to as pomace. Pomace (including filtration retentate) “as is” provides fiber, color, nutrients, mouthfeel, flavor.
It was discovered that the pomace may be treated to reduce the particle (including fiber) size resulting in improved texture and dispersion capability of the pomace in a product. It was further discovered that upon size reduction, the ratio of soluble portions of the pomace to insoluble portions increases, more flavors and colors are released, and the texture of the particles feels smoother.
Any suitable form of pomace such as wet pomace, frozen pomace, or dry pomace may be used to obtain the desired product. Wet or frozen pomace does not require rehydration. Dry pomace is lighter and easier to store. Wet pomace may be taken directly as the press cake. The moisture content of the pomace depends on the fruit or vegetable they may vary on moisture content. There are various methods to dry pomace if desired, for example on a commercial scale a continuous forced air indirect fire oven may be used.
The pH of the pomace may range from 2 to 7. Prior to particle size reduction, the pomace may be acidified to lower the pH. Typically the pomace is acidified to under pH 4.5 for microbial stability. Sufficient amounts of organic acids such as citric acid, lactic acid, acetic acid, malic acid, succinic acid, or juices such as lemon juice and/or acidic juice concentrate may be added to the pomace to reduce the pH of pomace to a desired pH.
Processing of Whole Fruits and Vegetables and/or Pomace
In accordance with one aspect, the whole fruits or vegetables intermediate product obtained by pre-treating the whole fruits or vegetables as discussed above or pomace material is subjected to a size-reduction process. Size reduction may occur through any suitable mechanical or chemical process such as micro-grinding, cutting, shredding, slicing, grinding, shearing, extruding, homogenizing, pulverizing, comminuting, or subjecting to ultrasonic frequency. In one aspect, the size reduction is achieved by micro-grinding.
Micro-grinding process is typically used for pureeing portions of fruits and vegetables such as fruits and vegetables wherein the skin, seeds, etc. are removed, but has not heretofore been known to reduce the size of whole fruits and vegetables or of pomace.
This process comprises feeding, grinding, and mesh-screening or filtering processes in a unit operation. Therefore, the outcome has more controlled and homogeneous size distribution than random grinding without mesh-screening process. The micro-grinding process provides a product which is very suitable for addition to food products, and in particular beverage products to provide high nutritional value. For further size reduction, the micro-ground product is, optionally, passed through pressurized homogenizers such as dairy or dipping sauce homogenizers. The micro-ground product can be incorporated in wet form or dehydrated to appropriate moisture level prior to use in product formulation.
Ideally, the particle size of the whole fruits and vegetables or pomace is reduced to at least below 250 microns to provide a micro-ground product with a smooth texture and dispersibility, while below 125 micron is recommended for the better sensory result. In some examples, the particle size may be reduced to below 75 micron or below 38 micron. The range of 38 micron to 125 micron particularly improves both dispersion and mouthfeel characteristics in beverages. Micro-grinding also increases: (1) total fiber content; and (2) the ratio of soluble fiber to insoluble fiber. This indicates that the mechanical grinding method partially converts: (1) non-detectable fiber to detectable by AOAC method; and (2) insoluble fiber to soluble fiber. See Example 2 below.
In another aspect of the invention, pomace is prepared for freeze-shearing by adding water to the pomace. The amount of water depends on the type of pomace. For example, cranberry pomace requires a 20:1 water:pomace ratio whereas blueberry pomace requires a 10:1 water to pomace ratio. The amount of water necessary to prepare the pomace for freeze shearing is easily determined by following the process outlined below in the examples. Essentially, sufficient water is added to form and maintain a vortex in a Vitamix or other suitable blender for about 5 minutes. When frozen, such hydrated pomace provides an ideal substrate for freeze shearing.
The mixture is then frozen at 0 to −20° C., for example −9° C., until suitably frozen, typically 12-20 hours. The frozen puree is then subjected to at least one cycle of shearing such as with the Pacojet shearing process. The result is a smooth frozen puree which, when eaten, exhibits little or no grittiness experienced with the untreated puree/slurry.
Upon thawing at room temperature, a smooth, thick puree with the consistency of dairy pudding can be obtained. The continuous hydration and shear of the pomace increases viscosity and film forming ability. The unexpected result indicates shear and hydration can be used to slightly modify fruit fiber to create a useful food ingredient.
Before or after size reduction, components that provide off-tastes may be removed from the fruit or vegetable intermediate product or from the pomace. The components (e.g. bitter nutrients such as certain phytonutrients for example) may be left in the fruits and vegetables or pomace, or may be removed before or after particle size reduction through steam-stripping or blenching, or some type of filtration such as micro, ultra, nano filtration or reverse osmosis and then replaced (e.g. after encapsulation via a typical shell and core or even replaced through an emulsion system or some other process to overcome the off-taste). Steam stripping or blenching can also eliminate undesirable enzymatic reactions including enzymatic browning, and also initial load of microorganisms.
If pH adjustment had not been made prior in the process (before particle size reduction) then sufficient amounts of organic acids such as citric acid, lactic acid, acetic acid, malic acid, succinic acid, or juices such as lemon juice and/or acidic juice concentrate may be added to the size-reduced product to reduce the pH as desired.
The size-reduced fruit or vegetable or pomace product may then be used to prepare a beverage or food product. If necessary, the micro-ground product may be subjected to partial dehydration to remove some of the moisture. The resulting products are combined with base juices to form a beverage formulation which optionally may be homogenized and then pasteurized.
The size-reduced fruit or vegetable or pomace product may be used as main ingredients of fruit and vegetable food products or may be included in various food products to provide enhanced nutrition and other characteristics, such as color, flavor, and mouthfeel. Suitable food products include, but are not limited to beverages, soups, spreads, puddings, smoothies, snack foods, yogurts, and cereals.
The size-reduced fruit or vegetable or pomace product may be added to products that usually contain little or no real fruit, such as gummy snacks that contain less than 2% fruit, or multiphase snacks with a jam filling that is mostly non-fruit ingredients such as fillings for toaster tarts and the like. The size-reduced fruit or vegetable or pomace product may be used as a substitute for a combination of fruit juice or juice concentrate with a thickener (gum) because it can provide both fruit benefits and viscosity.
The size-reduced fruit or vegetable or pomace product may be added to various types of beverages such as fruit and or vegetable juices, fruit smoothies, fruit beverages and fruit cocktails. This will enhance natural fiber and phytochemical contents and increase viscosity, smoothness and mouth filling.
The following table demonstrates the benefit of added pomace (or goo) to various base juices.
As shown in the following table, pomace ingredients (without treatment) are generally higher molecular weight and less soluble and dispersible than FIBERSOL-2 a modified corn starch by ADM or other common sources of fiber. However, treating pomace as described herein to reduce the particle size provides pomace having increased solubility and greater nutritional value.
Dietary fiber comprises the remnants of edible plants cells, polysaccharides, lignin, and associate substances (carbohydrates) resistant to (hydrolysis) digestion by alimentary enzymes of humans.
AOAC fiber analysis is used to detect the amount of fiber in a food ingredient. This is important so that the amount of dietary fiber may be accurately reported in an ingredient/nutritional content disclosure. In another aspect of the invention, the pomace is pre-heated to inactivate natural enzymes present in the fruits or vegetables ingredients present in the pomace. The pomace is pre-heated to a temperature of at least about 70° C., to about 100° C. The pre-heating occurs prior to AOAC fiber analysis to maximize the fiber detection. It was discovered that such pre-heating treatment results in increasing the measured fiber content of the material and modifying the structure of the fiber. The heating may be done prior to particle size reduction, afterwards, both, or neither.
Orange juice (200 g), carrot pomace treated to reduce the particle size (43 g), lemon Juice (2 g), and carrot goo (1 g) were combined to form a juice product. The resulting juice product had the following nutritional characteristics based on an 8 oz serving. Calories: 100; Total Fat: 0 g; Cholesterol 0 mg; Total Carb 25 g (Dietary Fiber 3 g; Sugars 20 g); Vitamin A: 220%; Vitamin C: 100%.
Wet orange pomace was used in various forms to compare measured fiber. AOAC method 991.43; All results are listed in dry basis.
Two samples of orange pomace were subjected to AOAC analysis. Oranges were peeled and then juiced in an electric juicer. The resulting pomace was washed with water. The first sample (control) had no heat treatment. The second sample was heated for 5 minutes in boiling water and then drained. Both samples were freeze dried. Fiber and pectin analysis was conducted on the sample solids.
It was concluded that inactivation of naturally existing enzymes (including pectinase) by heating increases fiber content.
Four gallons of cranberry fine pomace were mixed with cranberry juice. One part of this material was mixed with four parts concentrated juice and water (16 gallons). The resulting slurry was subjected to three steps of reduction. Step one used the 212084-1 microcut head for 125 micron product which step produced about 4″ of “foam” on top of the cranberry liquid. The result indicated a good reduction of fine cranberry pulp with cranberry “skins”.
Step two further processed the step one product into a 21608-1 microcut head for 75 micron product. Not much foam was produced in step two. The result indicated a good further reduction of cranberry pulp was accomplished in this step.
Step three further processed the step two product through a 216084 microcut head for 35 micron product. Almost no foam was produced in the last step. The result indicated a good final reduction of cranberry pulp in cranberry juice/water.
Fruit and vegetable pomace was reduced to as small as possible without having to add extra juice. Orange pulp was cut into 2″ pieces so that the pieces could be fed into a cutting head for further reduction. There was a very good reduction of the 2″ sized cubes of orange pulp into free flow 0.090″ pieces of orange pulp. This material fed easily into a microcut impeller having a 212084-1 microcut head. The resulting reduced orange juice pulp mixed well with water.
The carrot pulp was too thick as received. One part carrot juice (by weight) was added to one part carrot pulp and mixed well so that the material could go into the 216084-1 microcut head 35 micon. Moisture content (M.C.) 87.15%. There was a very good further reduction of carrot slurry into a fine puree which floated in water.
Cranberry pulp (skin and seeds) was mixed with 1 part cranberry juice to 1 part pulp and mixed well. The M.C. was 55.11%. The 1:1 cranberry pulp slurry was reduced in the 200084-2° microcut head and produced a dry ribbon of pulp. This ribbon extrusion is due to the cranberry seeds being reduced. More liquid or juice was added to this product to go into the next reduction step. Three parts of juice was added to make the input material 3:1 or 62.9% M.C.
The second step of reduction on the 3:1 cranberry slurry was successful. An additional trickle of water was needed to avoid a pause in the feeding of the cranberry pulp slurry which would result in high temperature in the microcut cutting zone and steam being released from the material being cut.
Because the material that goes into the 216084 microcut head should be pourable, water was added to the cranberry pulp slurry to make it a 5:1 slurry with 76.66% M.C. The 5 parts liquid to 1 part cranberry pulp was sufficient to reduce the cranberry pulp to as fine as could be produced. Some of the pulp floated and some sank to the bottom of the container.
A fine particle size fiber by dry grinding was created that reduces negative texture in foods and beverages. About 200 grams each of cranberry and blueberry pomace were ground for about 5 minutes in a “Vitamix” brand high shear blender. Next, the materials were sieved using a ROTAP machine to create about 50 grams of material that passed through a US 120 mesh sieve.
The dry grinding and sieve produced sufficient powder to pass through a 120 mesh powder. The powder had a greatly reduced gritty feel when rubbed between fingers.
The ability of a “freeze-shear” process to reduce the particle size and functional properties of cranberry and blueberry pomace was evaluated. In the culinary world, a process sometimes called “Paco-Tizing” is used be chefs to create smooth food purees. The process involves cooking of various foods, adding liquid to the food mixture, freezing the mixture, then subjecting to the “Paco-tizing” treatment. A blade is used to mechanically shave off thin shavings of ice from the frozen food—water matrix. The result is often a smooth purees than can be used for various culinary applications. The consistency of the resultant purees is often referred to as “baby-food”
It was discovered that applying this freeze-shear/shave process to a high fiber material creates a modified fiber with improved water sorbtion, texture, and food ingredient useful properties.
Fifty (50) grams each of cranberry pomace (Cran Chile) or blueberry pomace (Givaudan) was hydrated continuously with water in order to create a slurry or paste that could be frozen.
Try #1: 50 g of cold water was added to 50 g of cranberry pomace and mixed in a bowl (stainless steel) with a standard rubber spatula. The pomace immediately absorbed the water fully but did not produce a slurry. Water was added at 50 g increments to understand how much water must be added to make a slurry.
Try #2: Hot water (boiling) was used with the same procedure as in try #1 with virtually the same result.
Try #3a: 50 g Blueberry pomace and cold water and required only 10:1 water:pomace to achieve desired consistency.
Try #3b: A batch for freeze-shearing was prepared with a 20:1 water cranberry pomace ratio. In particular, 50 g cranberry pomace added to 500 g cold (20° C.) water in a Vitamix blender (a 10:1 ratio.) Blending on high, a vortex/sustained shearing could not be attained. An additional 100 g cold water (12:1) was added with still no vortex. Another 100 g cold water (14:1) was added and a vortex started and stopped erratically. An additional 100 g cold water (16:1) as added and a vortex began but after a minute, it ceased as viscosity of the blending appeared to increase. A further 100 g cold water (18:1) was added with the same result as the 16:1 ratio—a vortex was created for about 1-1½ minute, but then stopped and the viscosity of the mixture appeared to increase again. A final 100 g cold water (20:1) was added whereby a vortex was created and stayed for 5 minutes on high. After 5 minutes, mixture began to “dry pop” or “splash” as viscosity of mixture still continued to increase. Mixing was stopped.
A small amount of the mixture was spread on a culinary roule rubber mat and dried in a low oven (about 170° F.). After an hour, it was apparent that a film of fiber was forming indicating the fiber was modified enough by the Vitamix process to form a cohesive matrix. Unsheared material did not form a film.
The remaining balance of the mixture was frozen in 1 L stainless steel canisters (for later freeze-shearing) at 16° F. The next day, ˜16 hour later, the frozen puree was subject to two cycles of the Paoject shearing process. The result was immediately apparent: a smooth frozen puree resulted. When eaten, the puree exhibited little or no grittiness experienced with the untreated puree/slurry.
Upon thawing at room temperature, a smooth, thick puree with the consistency of dairy pudding was obtained. The pudding had only a slight degree of grittiness on the tongue and a slightly acidic/tart and astringent after taste (due to the acids and polyphenol compounds contained in cranberry pomace).
Continuous hydration and shear of cranberry and blueberry pomace increased viscosity and created film forming ability. Around 20 parts water to 1 part cranberry pomace or 10 parts water to 1 part blueberry pomace was needed to create a fluid that could be continuously sheared in a high shear Vitamix mixer/blender. The unexpected result indicated shear and hydration can be used to slightly modify fruit fiber to create a potentially useful food ingredient. Subsequent “freeze-shearing” created a relatively smooth pudding like product. The Vitamix or freeze-shear process alone did not have the same effect as the combination of the two treatments.
Samples Prepared:
A. Naked Juice blue machine fiber enrichment. Blended 15.2 g blueberry pomace that had been freeze-sheared after Vitamix hydration and sheer 1/6=n % s and contained 5 parts H2O 1 part pomace (12% total fiber based on calculation), 186.4 g Naked Juice Blue machine smoothie, and 38.4 g tap water. Thus 15.2 g×0.12 or 1.8 g fiber from pomace blend was prepared by shaking 10 seconds in a Naked Juice bottle. The resulting beverage had scant to no gritty texture.
B. Naked juice blue machine with cranberry pomace blend.
25 g sucrose (granulated), 0.34 g citric acid, and 0.16 g malic acid. Resulting blend had low to no gritty texture, low sweetness, and high tartness. Illustrated ability to add excellent source of fiber to a beverage.
C. Formula A above was prepared with 2× blueberry pomace. The blend provided a good source of fiber and some gritty texture.
D. Blended 6 oz of Yoplait blueberry yogurt with 21 g 5:1 blueberry pomace to obtain approximately a good source (2.5 g fiber/serving) fiber. Nice and smooth texture, no grit although flavor balance lost when added to commercial product.
E. Cranberry-fiber “pudding”/thick smoothie as prepared by combining 116 g (20:1) cranberry pomace “pudding,” 33 g sucrose, 0.5 g citric acid, 0.3 g malic acid, and 0.2 aspartame. The resulting smoothie had minimal grittiness. The acid and sweetener were balanced for more optimal taste.
While the invention has been described with respect to specific examples including presently preferred modes of carrying out the invention, those skilled in the art will appreciate that there are numerous variations and permutations of the above described systems and techniques that fall within the spirit and scope of the invention as set forth in the appended claims.
The application claims priority to U.S. provisional application Ser. No. 61/390,720 filed Oct. 7, 2010, hereby incorporated by reference in its entirety.
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