Claims
- 1. A method for separating a selected genetic material from a crude sample containing genetic material applied to a solid medium, the method consisting of:applying a cleaning solution of a water miscible solvent mixed with a source of pH in the range of 9.0 to 13.0 to the sample of genetic material on the solid medium the solution destroying RNA in the sample; applying an eluting solution to the sample of genetic material on the solid medium; and collecting the selected genetic material in the eluting solution.
- 2. The method according to claim 1 wherein the selected genetic material is further analyzed by polymerase chain reaction.
- 3. The method according to claim 1 wherein the selected genetic material is further analyzed by restriction fragment length polymorphism.
- 4. The method according to claim 1 wherein genetic material not removed from the solid medium is analyzed.
- 5. The method according to claim 4 wherein the genetic material not removed from the solid medium is analyzed by polymerase chain reaction in situ.
- 6. The method according to claim 1 wherein the solid medium comprises:a solid matrix; and a protein denaturing agent.
- 7. The method according to claim 6 further comprising a free radical trap.
- 8. The method according to claim 7 wherein the protein denaturing agent is a detergent and the free radical trap is uric acid or a urate salt.
- 9. The method according to claim 7 wherein the protein denaturing agent and free radical trap is guanidium thiocyanate.
- 10. The method according to claim 7 wherein the protein denaturing agent is guanidine hydrochloride and the free radical trap is uric acid or a urate salt.
- 11. The method according to claim 6 wherein the protein denaturing agent is a guanidium salt.
- 12. The method according to claim 7 wherein the free radical trap is guanidine thiocyanate.
- 13. The method according to claim 7 wherein the protein denaturing agent is urea and the free radical trap is uric acid or a urate salt.
- 14. The method according to claim 1 wherein the crude sample includes genetic material from multiple sources.
- 15. A kit for separating a selected genetic material from a sample, the kit consisting of:a solid medium; a cleaning solution of a water miscible solvent mixed with a source of pH in the range of 9.0 to 13.0, the solution destroying RNA in the sample; and an eluting solution.
- 16. The kit according to claim 15 wherein the cleaning solution comprises ethanol and sodium hydroxide.
- 17. The kit according to claim 15 wherein the eluting solution is a saline eluting solution.
- 18. The kit according to claim 17 wherein the saline solution comprises 10 mM ammonium acetate.
- 19. The kit according to claim 17 wherein the saline solution comprises 10 mM ammonium bicarbonate.
- 20. A method for separating genetic material of a plasmid from genetic material of a bacteria applied as a sample to a solid medium, the method consisting of:applying a sample including the bacteria containing the plasmid to a solid medium; applying a cleaning solution of a water miscible solvent mixed with a source of pH in the range of 9.0 to 13.0 to the sample on the solid medium the solution destroying RNA in the sample; applying a rinsing solution to the sample on the solid medium; applying an eluting solution to the sample on the solid medium; and collecting the genetic material of a selected one of the bacteria and plasmid in the eluting solution.
Parent Case Info
This application claims benefit of Provisional application Ser. No. 60/099,715. filed Sep. 10, 1998.
US Referenced Citations (4)
Non-Patent Literature Citations (1)
Entry |
Huynh et al. Sequential Elution of Denatured proteins, hydrolyzed RNA and Plasmid DNA of bacterial lysates absorbed onto Stached DEAE cellulose memebranes. Analytical Biochemistry. vol. 211, pp. 61-65, Dec. 1993. |
Provisional Applications (1)
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Number |
Date |
Country |
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60/099715 |
Sep 1998 |
US |