Claims
- 1. A process for the preparation of desacetyl cephalosporin C which comprises fermenting a cephalosporin C-producing microorganism in the presence of an amount of an acetylesterase enzyme effective to convert substantially all the cephalosporin C produced into desacetyl cephalosporin C before non-enzymic degradation of the cephalosporin C occurs.
- 2. The process of claim 1 wherein fermentation is carried out at from 15.degree.-45.degree. C. at a pH of from 4 to 9 for from 1 to 20 days.
- 3. The process of claim 1 wherein the cephalosporin C-producing microorganism is a strain of the species Acremonium chrysogenum.
- 4. The process of claim 1 wherein an excess of the acetylesterase enzyme is added before accretion of the cephalosporin C.
- 5. The process of claim 1 wherein the acetylesterase is derived from the bacterial genus Rhizobium, the yeast genus Rhodotorula or from the class Basidiomycetes, or species B. subtilis.
- 6. The process of claim 1 wherein the acetylesterase is derived from Rhodosporidium toruloides strain CBS 349.
- 7. The process of claim 1 in which the esterase is used in the form of a cell-free preparation.
- 8. The process of claim 1 wherein the acetylesterase is generated in situ by fermentation of the esterase-producing microorganism.
- 9. The process of claim 3 wherein the acetylesterase is generated in situ by the cephalosporin C-producing microorganism during the fermentation.
Priority Claims (1)
Number |
Date |
Country |
Kind |
7925283 |
Jul 1979 |
GBX |
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Parent Case Info
This is a continuation of application Ser. No. 171,929, filed July 18, 1980 and now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
3912589 |
Smith |
Oct 1975 |
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3926728 |
Kanzaki et al. |
Dec 1975 |
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Non-Patent Literature Citations (4)
Entry |
Konecny et al., J. of Antibiotics XXVI, No. 3, pp. 135-141 (1973). |
Movita et al., Phil. Trans. R. Soc. Lond. B289, pp. 181-190 (1980). |
Fujisawa et al., Ag. Biol. Chem. 39(6), pp. 1295-1301 (1975). |
Fujisawa et al., Agr. Biol. Chem. 39(6), pp. 1303-1309 (1975). |
Continuations (1)
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Number |
Date |
Country |
Parent |
171929 |
Jul 1980 |
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