Production of Adenovector Based Vaccines

Information

  • Research Project
  • 7747764
  • ApplicationId
    7747764
  • Core Project Number
    R44AI063819
  • Full Project Number
    2R44AI063819-03A1
  • Serial Number
    63819
  • FOA Number
    PA-06-134
  • Sub Project Id
  • Project Start Date
    3/15/2005 - 19 years ago
  • Project End Date
    7/31/2012 - 12 years ago
  • Program Officer Name
    KHAMBATY, FARUKH M.
  • Budget Start Date
    8/17/2009 - 15 years ago
  • Budget End Date
    7/31/2010 - 14 years ago
  • Fiscal Year
    2009
  • Support Year
    3
  • Suffix
    A1
  • Award Notice Date
    8/16/2009 - 15 years ago
Organizations

Production of Adenovector Based Vaccines

DESCRIPTION (provided by applicant): Adenovirus vectors (Advectors) designed to express an antigenic gene have shown great promise as vaccines. Although GenVec and other groups have constructed numerous Advectors expressing a variety of antigens a substantial subset of antigen genes have been found to be inhibitory to Advector construction and production. This limitation was resolved in our phase I research by blocking antigen expression with a repressor system that is over 50-fold stronger than the commonly used TetR system. The technology was demonstrated to be applicable to multiple serotype groups of Advectors. In this phase 2 SBIR grant application we propose to use our optimized repressor to make a production cell line that is broadly applicable to vaccine vectors based on different human serotype groups and encoding inhibitory antigens. At the end of this phase 2 research a cell line certified ready for use in GMP banking will be available. No such cell line presently exists. The strategy to make the preferred repressor cell line is described in the three aims of this proposal. In Aim 1 the preferred repressor cell line with an optimized expression cassette will be constructed. Development of an optimized expression cassette is required to circumvent the epigenetic silencing found with the expression cassette used in our phase 1 research. In Aim 2 the preferred repressor cell line will be identified and certified for use in GMP banking. Since it is anticipated that Advector vaccines will be produced in suspension cells for commercial purposes, in Aim 3 we will demonstrate that the preferred repressor cell line can be adapted to grow in suspension and support production of Advectors from multiple serotype groups with inhibitory antigens. The final GMP manufacturing cell line will speed and facilitate the testing of Advector vaccine concepts for many antigens, hasten their progress to clinical testing and reduce the cost of goods for future approved vaccine products. PUBLIC HEALTH RELEVANCE: Adenovirus vector-based vaccines show great promise for protecting against many human diseases. However, some promising vaccines can not be made. In this proposal we overcome this limitation by developing a cell line that ensures any Adenovirus vector vaccine can be produced.

IC Name
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
  • Activity
    R44
  • Administering IC
    AI
  • Application Type
    2
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    684896
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    855
  • Ed Inst. Type
  • Funding ICs
    NIAID:684896\
  • Funding Mechanism
    SBIR-STTR
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    GENVEC, INC.
  • Organization Department
  • Organization DUNS
    806729547
  • Organization City
    GAITHERSBURG
  • Organization State
    MD
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    208784021
  • Organization District
    UNITED STATES