Claims
- 1. A method to effect the conversion of substrates to products in an enzyme-catalyzed reaction which method comprises adding to a reaction mixture containing said substrates the seeds of a transgenic plant, wherein said plant has been modified to contain an expression system for the production of said enzyme, and wherein the enzyme is heterologous to the seed.
- 2. A method to effect the conversion of substrates to products in an enzyme-catalyzed reaction which method comprises adding to a reaction mixture containing said substrates the seeds of a transgenic plant, wherein said plant has been modified to contain an expression system for the production of said enzyme, and wherein the reaction mixture would not otherwise contain any seeds.
- 3. The method of claim 1 or 2 wherein the seeds are in milled form.
- 4. The method of claim 3 wherein the seeds are milled prior to said adding.
- 5. The method of claim 1 or 2 wherein said seeds are milled subsequent to said adding.
- 6. The method of claim 1 or 2 wherein the enzyme is a hydrolase enzyme.
- 7. The method of claim 6 wherein the hydrolase enzyme is a protease, a cellulase, a hemicellulase, a phosphatase, a lipase, a pectinase, an amylase, a lysozyme, a pellulanase, a phytase or a chitinase.
- 8. The method of claim 1 or 2 wherein the enzyme is a lyase.
- 9. The method of claim 8 wherein the lyase is pectinase.
- 10. The method of claim 1 or 2 wherein the enzyme is an isomerase.
- 11. The method of claim 10 wherein the isomerase is a glucose isomerase.
- 12. The method of claim 1 or 2 wherein said reaction is a digestive reaction and the seeds are added to the reaction mixture by adding said seeds to an animal food.
- 13. A reaction mixture containing the substrate of an in vitro enzyme-catalyzed reaction, which mixture further comprises seeds of a transgenic plant modified to contain an expression system for production of said enzyme in the seeds of the plant,
- wherein said enzyme is heterologous to said seeds.
- 14. A reaction mixture containing the substrate of an in vitro enzyme-catalyzed reaction, which mixture further comprises seeds of a transgenic plant modified to contain an expression system for production of said enzyme in the seeds of the plant and wherein said reaction mixture would otherwise not contain seed.
- 15. An animal feedstuff which comprises the seeds of a transgenic plant modified to contain an expression system for production in its seeds of an enzyme effective in aiding digestion of a substrate further contained in the feedstuff.
Priority Claims (1)
Number |
Date |
Country |
Kind |
91200688 |
Mar 1991 |
EPX |
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RELATED APPLICATIONS
This application is a Continuation-in-Part U.S. application Ser. No. 07/756,994, filed Sep. 11, 1991, abandoned, which is a Continuation-in Part of U.S. application Ser. No. 07/498,561, filed Mar. 23, 1990, abandoned.
US Referenced Citations (13)
Foreign Referenced Citations (1)
Number |
Date |
Country |
0380343 |
Aug 1980 |
EPX |
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
756994 |
Sep 1991 |
|
Parent |
498561 |
Mar 1990 |
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