Claims
- 1. A process for producing isomaltulose by enzymatic conversion of sucrose using dead immobilized cells of an isomaltulose-forming microorganism comprising continuously passing a pure sucrose solution having a concentration of from 45 to 75% by weight sucrose at a temperature of about 45.degree. to 65.degree. C. through a reactor filled with said dead, immobilized cells of an isomaltulose-forming microorganism so that the sucrose is converted to a product containing primarily isomaltulose.
- 2. A process for producing isomaltulose by enzymatic conversion of sucrose using dead, immobilized cells of an isomaltulose-forming microorganism comprising continuously passing a pure sucrose solution having a concentration of from 65 to 75% by weight sucrose at a temperature of about 45.degree. to 65.degree. C. through a reactor filled with said dead, immobilized cells of an isomaltulose-forming microorganism so that the sucrose is converted to a product containing primarily isomaltulose.
- 3. Process according to claim 1 or 2, wherein the microorganism is Protaminobacter rubrum (CBS 574.77), Serratia plymuthica (ATCC 15928), Serratia marcescens (NCIB 8285) or Leuconostoc mesenteroides (NRRL B-512F).
- 4. Process according to claim 3, wherein the immobilized cells are cross-linked with glutaraldehyde or a cyanuric halide.
- 5. Process according to claim 3, wherein the cells are immobilized by flocculation with a cationic flocculating agent.
- 6. Process according to claim 3, wherein the cells are immobilized by flocculation with chitosan.
- 7. Process according to claim 3, wherein the cells are immobilized by inclusion in a calcium alginate matrix.
- 8. Process according to claim 3, wherein the cells are immobilized by inclusion in cellulose diacetate or cellulose triacetate.
- 9. Process according to claim 3, wherein the cells are immobilized by inclusion in K-carrageenan gel.
- 10. Process according to claim 3, wherein the cells are immobilized by flocculation with a cationic or anionic flocculating agent or with a combination of the two.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3038219 |
Oct 1980 |
DEX |
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Parent Case Info
This is a continuation of application Ser. No. 290,083 filed Aug. 4, 1981, and now abandoned.
US Referenced Citations (5)
Foreign Referenced Citations (2)
Number |
Date |
Country |
1099 |
Mar 1979 |
EPX |
2063268 |
Jun 1981 |
GBX |
Non-Patent Literature Citations (1)
Entry |
Chibata, et al., Advances in Applied Microbiology, vol. XXII, 1977, pp. 1-9. |
Continuations (1)
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Number |
Date |
Country |
Parent |
290083 |
Aug 1981 |
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