Claims
- 1. An isolated or recombinant polynucleotide comprising a nucleic acid sequence encoding a Streptomyces lividins glucose isomerase variant, the nucleic acid sequence comprising any of SEQ ID NOs:9-28.
- 2. An isolated or recombinant polynucleotide comprising a nucleic acid sequence encoding a Streptomyces lividins glucose isomerase variant, the nucleic acid sequence selected from the group consisting of SEQ ID NOS:9-28.
- 3. A library of isolated or recombinant polynucleotides comprising a nucleic acid sequence encoding a Streptomyces lividins glucose isomerase variant, the library comprising the nucleic acid sequences of SEQ ID NOS:9-28.
- 4. An expression cassette comprising the polynucleotide comprising a nucleic acid sequence encoding a Streptomyces lividins glucose isomerase variant of claim 1.
- 5. A kit comprising the polynucleotide comprising a nucleic acid sequence encoding a Streptomyces lividins glucose isomerase variant of claim 1.
- 6. A DNA construct comprising at least three transcriptional terminators and at least one cloning site situated between any two transcriptional terminators.
- 7. The DNA construct of claim 6 wherein the transcriptional terminators are tonB, thrA, or aspA, and the cloning sites are selected from the group consisting of AvrII, NheI, BfaI, Cac8I, BsaJI, and StyI.
- 8. A library of DNA constructs, each DNA construct comprising at least three transcriptional terminators and at least one cloning site situated between any two transcriptional terminators.
- 9. A DNA construct selected from the group consisting of a) A pSYCO109mcs plasmid consisting of SEQ ID NO:30,
b) A DNA construct for the short 1.5 GI promoter consisting of SEQ ID NO:31, c) A DNA construct for the short 1.20 GI promoter consisting of SEQ ID NO:32, d) A DNA construct for the pAH105 plasmid consisting of SEQ ID NO:70, e) A DNA construct for the pSYCO101 plasmid consisting of SEQ ID NO:71, f) A DNA construct for the pSYCO103 plasmid consisting of SEQ ID NO:72, g) A DNA construct for the pSYCO106 plasmid consisting of SEQ ID NO:73, h) A DNA construct for the pSYCO109 plasmid consisting of SEQ ID NO:74, i) A DNA construct for the pSCYO106mcs plasmid consisting of SEQ ID NO:78, and j) A DNA construct for the pRJ50 plasmid consisting of SEQ ID NO:79.
- 10. A vector having a multiple cloning site containing restriction recognition site sequences specific for the restriction endonucleases AscI, NheI, PacI, RsrII, NsiI, SacII, MluI, AgeI, SapI, and SnaBI.
- 11. The vector of claim 10 wherein the multiple cloning site has the nucleotide sequence of SEQ ID NO:77.
- 12. A transformed host cell comprising the polynucleotide encoding a Streptomyces lividins glucose isomerase variant, of claim 1 or claim 9.
- 13. The transformed host cell of claim 12 wherein the host cell is RJ8n.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Application No. 60/374,931, filed Apr. 22, 2002.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60374931 |
Apr 2002 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
10420587 |
Apr 2003 |
US |
Child |
10739542 |
Dec 2003 |
US |