Claims
- 1. A protein adsorbent suitable for use in the chromatographic separation of complex biological molecules which comprises a coloured compound having the formula:
- A--Y--B
- or,
- Y'--B
- wherein A is a cationic group selected from (i) an acyclic or cyclic fully substituted ammonium group of the formulae: ##STR4## wherein R.sup.1 to R.sup.4 are alkyl, alkenyl or aryl groups, B represents an aromatic radical and D represents an alicyclic radial in which at least one of R.sup.1 or R.sup.4, B and D contains a free valency, and (ii) an amidinium or guandiinium group of the formula: ##STR5## wherein R.sup.6 to R.sup.9 are H or alkl, alkenyl or aryl groups and n is 0 or 1; Y is a chromophore; Y' is a cationic chromophore; and B is a mono- or poly-halogenoheterocycle selected from dichloropyrimidinyl, difluoropyrimidinyl, trichloropyrimidinyl, 5-chlorodifluoropyrimidinyl, 5-cyanodichloropyrimidinyl, dichlorotriazinyl and monochlorotriazinyl bound to an inert substrate.
- 2. An adsorbent according to claim 1 wherein the chromophore is an azo chromophore.
- 3. An adsorbent according to claim 1 wherein the cationic chromophore is selected from
- 4-(3- & 4-trimethylammoniophenylazo)-aniline,
- 4-(3- & 4-guanidinophenylazo)-3-methylphenylamino,
- 4-(3'- & 4'-amidinophenylazo)phenylamino,
- 4-(3- & 4-amidinophenyl-azo)-3-methylphenylamino,
- 4-(3- & 4-amidinophenylazo)-2,5-dimethylphenylamino,
- 4-(3- & 4-amidinophenylazo)-5-methyl-2-methoxyphenylamino,
- 4-(3- & 4-amidinophenylazo)-2,5-dimethoxyphenylamino,
- 4-(3- & 4-amidinophenylazo)-3-acetylaminoaniline,
- 4-(3- & 4-amidinophenylazo)-3-ureidophenylamino,
- 4-(3- & 4-amidinophenylazo)-6-methoxy-3-acetylaminophenylamino,
- 4-(3- & 4-amidinophenylazo)-N-methylphenylamino,
- 4-([4-guanidinophenylazo]-[acetyl]-acetylamino)-phenylamino,
- 4-[4-(4-trimethylammoniophenylazo)-3-methylpyrazol-5-on-1-yl]-phenylamino,
- 4-[4-(4-amidinophenylazo)-3-methylpyrazol-5-on-1-yl]-phenylamino and
- 4-[4-(4-guanidinophenylazo)-3-methylpyrazol-5-on-1-yl]-phenylamino.
- 4. In the chromatographic separation of proteinaceous material by contacting said material with an adsorbent, the improvement which comprises using as the adsorbent, the coloured compound of claim 1.
Priority Claims (2)
Number |
Date |
Country |
Kind |
8517778 |
Jul 1985 |
GBX |
|
8613201 |
May 1986 |
GBX |
|
Parent Case Info
This is a continuation application of Ser. No. 880,662, filed June 30, 1986, now abandoned.
US Referenced Citations (6)
Foreign Referenced Citations (3)
Number |
Date |
Country |
61-7358 |
Jan 1986 |
JPX |
518340 |
Mar 1972 |
CHX |
1045685 |
Oct 1986 |
GBX |
Non-Patent Literature Citations (1)
Entry |
Gribnau, T. C. J., Coupling of Effector-Molecules to Solid Supports, Drukkerij van Nameren B.V.--Mijmegen, The Netherlands, 1977, pp. 22-29, 94-103, 161-181. |
Continuations (1)
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Number |
Date |
Country |
Parent |
880662 |
Jun 1986 |
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