Claims
- 1. A reagent for use in protein assay which comprises the dye: ##STR2## and an acid having a pKa of from 0 to 4.
- 2. The reagent of claim 1, wherein the acid has a pKa of from 1 to 2.
- 3. The reagent of claim 1, wherein the acid is phosphoric acid.
- 4. The reagent of claim 1, wherein the pH of the resultant solution is from -1 to 1.
- 5. The reagent of claim 1, wherein the concentration of said dye is from 0.001 to 0.1% (w/v) and of said acid is from 4 to 12% (w/v).
- 6. The reagent of claim 1, which further comprises an alcohol.
- 7. The reagent of claim 6, which contains from 0 to 10% (w/v) of said alcohol.
- 8. The reagent of claim 6, wherein said alcohol is ethanol.
- 9. The reagent of claim 6, which contains from 0.005 to 0.05% (w/v) of said dye, from 7.5 to 9.5% (w/v) of phosphoric acid and from 4 to 5% (w/v) of ethanol.
- 10. A method of assaying protein which comprises mixing a reagent comprising the dye ##STR3## and an acid having a pKa of from 0 to 4 and a protein-containing sample and observing the resultant color change.
- 11. The method of claim 10, wherein said change is monitored spectrophotometrically at a wavelength of from 570 to 620 nm.
- 12. The method of claim 10, wherein from 0.1 to 1 ml of said reagent is added for up to 25 .mu.g of protein in said sample.
- 13. The method of claim 10, wherein the protein is selected from the group consisting of albumin, hemoglobin, globulin, chymotrypsinogen A and cytochrome C.
- 14. The method of claim 10, wherein the protein containing sample is urine.
- 15. The method of claim 10, wherein the protein-containing sample is spinal fluid.
- 16. The method of claim 10, wherein the acid is phosphoric acid.
- 17. The method of claim 10, wherein the pH of the resultant solution is from -1 to 1.
- 18. The method of claim 10, wherein the concentration of said dye is from 0.001 to 0.1% (w/v) and of said acid is from 4 to 12% (w/v).
- 19. The method of claim 10, wherein said reagent further comprises an alcohol.
- 20. The method of claim 19, wherein said reagent contains from 0 to 10% (w/v) of said alcohol.
- 21. The method of claim 19, wherein said alcohol is ethanol.
- 22. The method of claim 10, wherein an automated analyzer is employed.
- 23. A method of assaying protein which comprises mixing a reagent which contains from 0.005 to 0.05% (w/v) of ##STR4## from 7.5 to 9.5% (w/v) of phosphoric acid and from 4 to 5% (w/v) of ethanol, and a protein containing sample and observing the resultant color change.
Government Interests
The invention described herein was made in the course of work under a grant or award from the Department of Health, Education and Welfare.
US Referenced Citations (4)