Claims
- 1. A method for generating a secondary library of scaffold protein variants comprising:
a) receiving a library of primary sequences generated utilizing an alignment program; b) generating a probability distribution table of amino acid residues in a plurality of variant positions from said primary sequences; c) combining a plurality of said amino acid residues to generate a secondary library of secondary sequences, wherein at least one of said secondary sequences is different from said primary sequences; and d) computationally ranking said secondary library.
- 2. A method according to claim 1, wherein said alignment program is a sequence alignment program.
- 3. A method according to claim 1, wherein said alignment program is a structural alignment program.
- 4. A method according to claim 1 further comprising synthesizing a plurality of said secondary sequences.
- 5. A method according to claim 4 wherein said synthesizing is done by multiple PCR with pooled oligonucleotides.
- 6. A method according to 5 wherein said pooled oligonucleotides are added in equimolar amounts.
- 7. A method according to claim 5 wherein said pooled oligonucleotides are added in amounts that correspond to the frequency of the mutation.
- 8. A method according to claim 6 wherein said pooled oligonucleotides are pooled in relative amounts.
PRIORITY
[0001] This application is a divisional application of U.S. Ser. No. 09/782,004, filed Feb. 12, 2001, which claims the benefit of the filing date of U.S. S. No. 60/181,630, filed Feb. 10, 2000, U.S. S. No. 60/186,904, filed Mar. 3, 2000 and U.S. S. No. 60/197,851, filed Apr. 14, 2000.
Provisional Applications (3)
|
Number |
Date |
Country |
|
60181630 |
Feb 2000 |
US |
|
60186904 |
Mar 2000 |
US |
|
60197851 |
Apr 2000 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09782004 |
Feb 2001 |
US |
Child |
10666311 |
Sep 2003 |
US |