Claims
- 1. An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
a) a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, or SEQ ID NO:31; b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32; and c) a polynucleotide complementary to a polynucleotide of any of a) through b) above.
- 2. An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32.
- 3. An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 92% sequence identity with a polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6.
- 4. An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32.
- 5. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:3.
- 6. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:6.
- 7. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:8.
- 8. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:10.
- 9. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:12.
- 10. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:14.
- 11. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:16.
- 12. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:18.
- 13. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:20.
- 14. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:22.
- 15. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:24.
- 16. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:26.
- 17. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:28.
- 18. The PPSRP coding nucleic acid of claim 1, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:32.
- 19. A vector comprising the PPSRP coding nucleic acid of claim 1.
- 20. An isolated Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid selected from the group consisting of a polynucleotide sequence encoding a PP2A-1 and a PP-1 from Physcomitrella patens; a BnPP2A-1, a BnPP2A-2, and a BnPP2A-3 from Brassica napus; a GmPP2A-1, a GmPP2A-2, a GmPP2A-3, a GmPP2A-4, and a GmPP2A-5 from Glycine max; and an OsPP2A-1, an OsPP2A-2, an OsPP2A-3, an OsPP2A-4, and an OsPP2A-5 from Oryza sativa.
- 21. A transgenic plant cell comprising the PPSRP coding nucleic acid of claim 1.
- 22. A transgenic plant comprising a plant cell of claim 21.
- 23. The plant of claim 22, wherein the plant is a monocot.
- 24. The plant of claim 22, wherein the plant is a dicot.
- 25. The plant of claim 22, wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grass and a forage crop.
- 26. A plant seed produced by the plant of claim 22.
- 27. The seed of claim 26, wherein the seed is true breeding for an increased tolerance to an environmental stress as compared to a wild type variety of the seed.
- 28. A method of modulating a plant's tolerance to an environmental stress comprising, modifying the expression of a Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid in the plant, wherein the nucleic acid is selected from the group consisting of:
a) a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, or SEQ ID NO:31; b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, or SEQ ID NO:32; c) a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32; d) a polynucleotide encoding a polypeptide having at least 92% sequence identity with a polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6; e) a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32; f) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) through e) above; and g) a polynucleotide complementary to a polynucleotide of any of a) through f) above.
- 29. The method of claim 28, wherein the environmental stress is drought and/or increased salinity.
- 30. The method of claim 28, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:3.
- 31. The method of claim 28, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:6.
- 32. The method of claim 28, wherein the nucleic acid comprises at least 60 consecutive nucleotides of any of the polynucleotides of a) through e) of claim 28.
- 33. The method of claim 28, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32.
- 34. The method of claim 28, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 92% sequence identity with the polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6.
- 35. The method of claim 28, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32.
- 36. The method of claim 28, wherein the plant's stress tolerance is increased by increasing expression of the nucleic acid in the plant.
- 37. The method of claim 28, wherein the nucleic acid encodes a polypeptide that functions as a protein phosphatase.
- 38. The method of claim 28, wherein the plant is not transgenic.
- 39. The method of claim 28, wherein the plant is transgenic.
- 40. The method of claim 39, wherein the plant is transformed with a promoter that directs expression of the nucleic acid.
- 41. The method of claim 40, wherein the promoter is tissue specific.
- 42. The method of claim 40, wherein the promoter is developmentally regulated.
- 43. A method of producing a transgenic plant containing a Protein Phosphatase Stress-Related Polypeptide (PPSRP) coding nucleic acid wherein the plant has an increased tolerance to an environmental stress as compared to a wild type variety of the plant comprising, transforming a plant cell with an expression vector comprising the nucleic acid and generating from the plant cell the transgenic plant, wherein the nucleic acid is selected from the group consisting of:
a) a polynucleotide as defined in SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, or SEQ ID NO:31; b) a polynucleotide encoding a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, or SEQ ID NO:32; c) a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32; d) a polynucleotide encoding a polypeptide having at least 92% sequence identity with a polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6; e) a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32; f) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) through e) above; and g) a polynucleotide complementary to a polynucleotide of any of a) through f) above.
- 44. The method of claim 43, wherein the environmental stress is drought and/or increased salinity.
- 45. The method of claim 43, wherein the plant is a monocot.
- 46. The method of claim 43, wherein the plant is a dicot.
- 47. The method of claim 43, wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grass and a forage crop.
- 48. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:3.
- 49. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:6.
- 50. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:8.
- 51. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:10.
- 52. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:12.
- 53. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:14.
- 54. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:16.
- 55. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:18.
- 56. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:20.
- 57. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:22.
- 58. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:24.
- 59. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:26.
- 60. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:28.
- 61. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:30.
- 62. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding the polypeptide of SEQ ID NO:32.
- 63. The method of claim 43, wherein the nucleic acid comprises at least 60 consecutive nucleotides of any of the polynucleotides of a) through e) of claim 43.
- 64. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 97% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:26, SEQ ID NO:28, or SEQ ID NO:32.
- 65. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 92% sequence identity with a polypeptide as defined in SEQ ID NO:3 or SEQ ID NO:6.
- 66. The method of claim 43, wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 90% sequence identity with a polypeptide as defined in SEQ ID NO:3, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, or SEQ ID NO:32
- 67. The method of claim 43, wherein the plant's stress tolerance is increased by increasing expression of the nucleic acid in the plant.
- 68. The method of claim 43, wherein the nucleic acid encodes a polypeptide that functions as a protein phosphatase.
- 69. The method of claim 43, wherein the plant is transformed with a promoter that directs expression of the nucleic acid.
- 70. The method of claim 69, wherein the promoter is tissue specific.
- 71. The method of claim 69, wherein the promoter is developmentally regulated.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present invention claims the priority benefit of U.S. Provisional Patent Application Serial No. 60/317,305 filed Sep. 5, 2001, the entire contents of which are hereby incorporated by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60317305 |
Sep 2001 |
US |