Claims
- 1. A mammalian cell lacking type-1 parathyroid hormone (PTH)/parathyroid hormone-related peptide (PTHrP) receptor (PTH1R) activity and containing carboxyl-terminal parathyroid hormone receptor (CPTHR) activity.
- 2. The cell of claim 1, wherein said cell is homozygous for the ablation of the PTH1R gene.
- 3. The cell of claim 1, wherein said cell is an osteocyte.
- 4. The cell of claim 1, wherein said cell is an osteoblast.
- 5. A method of screening for agonists or antagonists for carboxyl-terminal parathyroid hormone receptor (CPTHR), said method comprising:
(a) contacting a mammalian cell lacking type-1 parathyroid hormone (PTH)/parathyroid hormone-related peptide (PTHrP) receptor (PTH1R) activity and containing CPTHR activity with a candidate compound; and (b) determining the rate of apoptosis of said cell, wherein an accelerated rate of apoptosis compared to said cell not contacted with a candidate compound indicates that said compound is an agonist for CPTHR, and wherein a rate of apoptosis equal to or less than said cell not contacted with a candidate compound indicates that said compound is an antagonist for CPTHR.
- 6. The method of claim 5, wherein said cell is homozygous for the ablation of the PTH1R gene.
- 7. The method of claim 5, wherein said cell is an osteocyte.
- 8. The method of claim 5, wherein said cell is an osteoblast.
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY-SPONSORED RESEARCH AND DEVELOPMENT
[0001] Part of the work performed during development of this invention utilized U.S. Government funds. The U.S. Government has certain rights in this invention.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/US01/25355 |
8/14/2001 |
WO |
|