Claims
- 1. An antibody directed against an isolated and purified soybean enzyme, wherein said enzyme adds a β-1,2-linked xylose to the β-linked mannose on the N-linked oligosaccharides of storage glycoproteins.
- 2. The enzyme of claim 1, wherein said enzyme is β1,2-xylosyltransferase.
- 3. The antibody of claim 1, wherein digestion of said enzyme by Endo lys C produces a set of proteolytic fragments, said set of proteolytic fragments having a set of sequences comprising SEQ ID NOs. 1-3 or a set of sequences comprising SEQ ID NOs: 4-5.
- 4. The antibody of claim 1, wherein said enzyme is obtained from the microsomal fraction of soybean cells.
- 5. The antibody of claim 1, wherein said enzyme has a purity of about 51,000-fold to near homogeneity compared to a crude microsomal homogenate.
- 6. The antibody of claim 1, wherein said enzyme is separated on SDS gels into 56 and 59 kDa bands.
- 7. A method for localizing xylosyltransferase in a plant cell, comprising the steps of:
contacting said cell with the antibody of claim 1; and detecting localization of said antibody, wherein the localization of said antibody indicating the localization of said enzyme.
- 8. A method for determining distribution levels of xylosyltransferase in a plant, comprising the steps of:
contacting said plant with the antibody of claim 1; and detecting binding activity of said antibody, wherein a higher activity of said antibody indicating a higher level of said xylosyltransferase in said plant.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This patent application is a divisional of U.S. Ser. No. 09/748,578, filed on Dec. 22, 2000, which is a divisional of U.S. Ser. No. 09/207,223, filed on Dec. 8, 1998, now issued as U.S. Pat. No. 6,168,937 on Jan. 2, 2001, which claims benefit of provisional patent application U.S. Serial No. 60/070,418, filed Jan. 5, 1998, now abandoned, and provisional application U.S. Ser. No. 60/067,932, filed Dec. 8, 1997, now abandoned.
Provisional Applications (2)
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Number |
Date |
Country |
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60070418 |
Jan 1998 |
US |
|
60067932 |
Dec 1997 |
US |
Divisions (2)
|
Number |
Date |
Country |
Parent |
09748578 |
Dec 2000 |
US |
Child |
10411905 |
Apr 2003 |
US |
Parent |
09207223 |
Dec 1998 |
US |
Child |
09748578 |
Dec 2000 |
US |