Claims
- 1. A biologically pure culture of Actinomadura verrucaspora subspecies veractimyces having the identifying characteristics of ATCC 39363, which culture is capable of producing the antibiotic CL-1577A and CL-1577B compounds in recoverable amounts under conditions of aerobic fermentation in a culture medium containing assimilable sources of carbon and nitrogen;
- said CL-1577A compound characterized by:
- (a) a molecular weight (FAB mass spectrometry) of 1248 a.m.u.
- (b) an ultraviolet absorption spectrum in methanol solution exhibiting absorption maxima at 215 nm (a=27.4), 252 nm (a=28.2), 282 nm (a=16.7), and 318 nm (a=11.6);
- (c) an infrared absorption spectrum (potassium bromide pellet) exhibiting principal absorption peaks at 3450, 2970, 2930, 1690, 1630, 1610, 1600, 1525, 1465, 1450, 1410, 1370, 1310, 1250, 1210, 1155, 1075, 1020, 990, 905, 880, 855, 795, 780, and 750 reciprocal centimeters;
- (d) a 200 MHz proton magnetic resonance spectrum (CDCl.sub.3) exhibiting signals at 1.11 (doublet), 1.33 (doublet), 1.35 (doublet), 1.41 (doublet), 1.56 (multiplet), 1.95 (doublet), 2.12 (multiplet), 2.13 (singlet), 2.22 (multiplet), 2.30 (multiplet), 2.52 (doublet of doublets), 2.53 (singlet), 2.75 (multiplet), 3.42 (singlet), 3.4-4.2 (multiplet), 3.81 (singlet), 3.89 (singlet), 3.98 (singlet), 4.21 (singlet), 4.57 (doublet), 4.67 (multiplet), 4.97 (doublet), 5.40 (multiplet), 5.50 (multiplet), 5.70 (doublet), 5.82 (doublet), 6.00 (doublet), 6.15 (broad singlet), 6.26 (singlet), 6.60 (doublet of doublets), 7.49 (singlet), 8.60 (singlet), and 11.71 (singelt) parts per million downfield from tetramethylsilane;
- (e) an optical rotation [.alpha.].sub.D =-198.degree. (c=1.25, CHCl.sub.3); and
- (f) the elemental analysis, 53.26% C, 6.04% H, 3.82% N, and 8.98% S;
- said CL-1577B compound characterized by:
- (a) an ultraviolet absorption spectrum in methanol solution exhibiting absorption maxima at 215 nm (a=26.8), 282 nm (a=16.1), and 325 nm (a=11.9);
- (b) an infrared spectrum (potassium bromide pellet) exhibiting principal absorption peaks at 3450, 2970, 2930, 1690, 1630, 1610, 1600, 1525, 1465, 1450, 1410, 1370, 1310, 1250, 1210, 1155, 1075, 1020, 945, 905, 880, 855, 795, 780, and 750 reciprocal centimeters;
- (c) a 200 MHz proton magnetic resonance spectrum (CDCl.sub.3) exhibiting signals at 1.11 (doublet), 1.12 (doublet), 1.24 (doublet), 1.35 (doublet), 1.41 (doublet), 1.43 (singlet), 1.46 (multiplet), 2.00 (multiplet), 2.13 (singlet), 2.14 (multiplet), 2.31 (multiplet), 2.52 (singlet), 2.53 (multiplet), 2.75 (multiplet), 3.42 (singlet), 3.4-4.2 (multiplet), 3.79 (singlet), 3.89 (singlet), 3.99 (singlet), 4.19 (singlet), 4.49 (multiplet), 4.56 (doublet), 4.66 (doublet), 4.77 (multiplet), 4.97 (doublet), 5.35 (broad singlet), 5.47 (multiplet), 5.72 (doublet), 5.83 (doublet), 5.93 (doublet), 6.21 (multiplet), 6.24 (singlet), 6.59 (doublet of doublets), 7.61 (singlet), 8.64 (singlet), and 11.58 (singlet) parts per million downfield from tetramethylsilane;
- (d) an optical rotation [.alpha.].sub.D =-189.degree. (c=0.63, CHCl.sub.3); and
- (e) the elemental analysis, 54.81% C, 6.33% H, 3.86% N, and 8.96% S.
Parent Case Info
This is a division of application Ser. No. 512,088, filed July 8, 1983, now U.S. Pat. No. 4,530,835.
US Referenced Citations (4)
Number |
Name |
Date |
Kind |
4195079 |
Celmer et al. |
Mar 1980 |
|
4301248 |
Nettleton, Jr. et al. |
Nov 1981 |
|
4546084 |
Konishi et al. |
Oct 1985 |
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Divisions (1)
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Number |
Date |
Country |
Parent |
512088 |
Jul 1983 |
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