Patent Application
20170081331
The present invention relates to a compound having the general formula (IIa) or (IIb), optionally in the form of a pharmaceutically acceptable salt, solvate, polymorph, codrug, cocrystal, prodrug, tautomer, racemate, enantiomer, or diastereomer or mixture thereof,
which is useful in treating, ameliorating or preventing a viral disease. Furthermore, specific combination therapies are disclosed.
In recent years the serious threat posed by influenza virus infection to worldwide public health has been highlighted by, firstly, the ongoing level transmission to humans of the highly pathogenic avian influenza A virus H5N1 strain (63% mortality in infected humans, http://www.who.int/csr/disease/avian_influenza/en/) and secondly, the unexpected emergence in 2009 of a novel pandemic influenza virus strain A/H1N1 that has rapidly spread around the entire world (http://www.who.int/csr/disease/swineflu/en/). Whilst the new virus strain is highly contagious but currently generally results in relatively mild illness, the future evolution of this virus is unpredictable. In a much more serious, but highly plausible scenario, H5N1 and related highly pathogenic avian influenza viruses could acquire mutations rendering them more easily transmissible between humans or the new A/H1N1 could become more virulent and only a single point mutation would be enough to confer resistance to oseltamivir (Neumann et al., Nature, 2009 (18; 459(7249) 931-939)); as many seasonal H1N1 strains have recently done (Dharan et al., The Journal of the American Medical Association, 2009 Mar. 11; 301 (10), 1034-1041; Moscona et al., The New England Journal of Medicine, 2009 (Mar. 5; 360(10) pp 953-956)). In this case, the delay in generating and deploying a vaccine (˜6 months in the relatively favourable case of A/H1N1 and still not a solved problem for H5N1) could have been catastrophically costly in human lives and societal disruption.
It is widely accepted that to bridge the period before a new vaccine is available and to treat severe cases, as well as to counter the problem of viral resistance, a wider choice of anti-influenza drugs is required. Development of new anti-influenza drugs has therefore again become high priority, having been largely abandoned by the major pharmaceutical companies once the neuraminidase inhibitors became available.
An excellent starting point for the development of antiviral medication is structural data of essential viral proteins. Thus, the crystal structure determination of e.g. the influenza virus surface antigen neuraminidase (Von Itzstein, M. et al., (1993), Nature, 363, pp. 418-423) led directly to the development of neuraminidase inhibitors with antiviral activity preventing the release of virus from the cells, however, not the virus production itself. These and their derivatives have subsequently developed into the anti-influenza drugs, zanamivir (Glaxo) and oseltamivir (Roche), which are currently being stockpiled by many countries as a first line of defence against a possible pandemic. However, these medicaments only provide a reduction in the duration of the clinical disease. Alternatively, adamantanes, the other class of licenced anti-influenza drugs (e.g. amantadine and rimantadine) target the viral M2 ion channel protein, which is located in the viral membrane interfering with the uncoating of the virus particle inside the cell. However, they have not been extensively used due to their side effects and the rapid development of resistant virus mutants (Magden, J. et al., (2005), Appl. Microbiol. Biotechnol., 66, pp. 612-621). In addition, more unspecific viral drugs, such as ribavirin, have been shown to work for treatment of influenza and other virus infections (Eriksson, B. et al., (1977), Antimicrob. Agents Chemother., 11, pp. 946-951). However, ribavirin is only approved in a few countries, probably due to severe side effects (Furuta et al., ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, p. 981-986). Clearly, new antiviral compounds are needed, preferably directed against different targets.
Influenza virus as well as Thogotovirus and isavirus belong to the family of Orthomyxoviridae which, as well as the family of the Bunyaviridae, including the Hantavirus, Nairovirus, Orthobunyavirus, and Phlebovirus, are, amongst others, negative stranded RNA viruses. Their genome is segmented and comes in ribonucleoprotein particles that include the RNA dependent RNA polymerase which carries out (i) the initial copying of the single-stranded negative-sense viral RNA (vRNA) into viral mRNAs (i.e. transcription) and (ii) the vRNA replication. This enzyme, a trimeric complex composed of subunits PA, PB1 and PB2, is central to the life cycle of the virus since it is responsible for the replication and transcription of viral RNA. In previous work the atomic structure of two key domains of the polymerase, the mRNA cap-binding domain in the PB2 subunit (Guilligay et al., Nature Structural & Molecular Biology 2008; May; 15(5): 500-506) and the endonuclease-active site residing within the PA subunit (Dias et al., Nature 2009, 458, 914-918) have been identified and their molecular architecture has been characterized. These two sites are critical for the unique “cap-snatching” mode used to initiate mRNA transcription that is used by the influenza virus and certain other virus families of this genus to generate viral mRNAs. A 5′ cap is a modified guanine nucleotide that has been added to the 5′ end of a messenger RNA. The 5′ cap (also termed an RNA cap or RNA m7G cap) consists of a terminal 7-methylguanosine residue which is linked through a 5′-5′-triphosphate bond to the first transcribed nucleotide. The viral polymerase binds to the 5′ RNA cap of cellular mRNA molecules and cleaves the RNA cap together with a stretch of 10 to 15 nucleotides. The capped RNA fragments then serve as primers for the synthesis of viral mRNA (Plotch, S. J. et al., (1981), Cell, 23, pp. 847-858; Kukkonen, S. K. et al (2005), Arch. Virol., 150, pp. 533-556; Leahy, M. B. et al., (2005), J. Virol., 71, pp. 8347-8351; Noah, D. L. et al., (2005), Adv. Virus Res., 65, pp. 121-145).
The polymerase complex seems to be an appropriate antiviral drug target since it is essential for synthesis of viral mRNA and viral replication and contains several functional active sites likely to be significantly different from those found in host cell proteins (Magden, J. et al., (2005), Appl. Microbiol. Biotechnol., 66, pp. 612-621). Thus, for example, there have been attempts to interfere with the assembly of polymerase subunits by a 25-amino-acid peptide resembling the PA-binding domain within PB1 (Ghanem, A. et al., (2007), J. Virol., 81, pp. 7801-7804). Furthermore, the endonuclease activity of the polymerase has been targeted and a series of 4-substituted 2,4-dioxobutanoic acid compounds has been identified as selective inhibitors of this activity in influenza viruses (Tomassini, J. et al., (1994), Antimicrob. Agents Chemother., 38, pp. 2827-2837). In addition, flutimide, a substituted 2,6-diketopiperazine, identified in extracts of Delitschia confertaspora, a fungal species, has been shown to inhibit the endonuclease of influenza virus (Tomassini, J. et al., (1996), Antimicrob. Agents Chemother., 40, pp. 1189-1193). Moreover, there have been attempts to interfere with viral transcription by nucleoside analogs, such as 2′-deoxy-2′-fluoroguanosine (Tisdale, M. et al., (1995), Antimicrob. Agents Chemother., 39, pp. 2454-2458).
It is an object of the present invention to identify further compounds which are effective against viral diseases and which have improved pharmacological properties.
Sequence of the de novo synthesized viral mRNA used for Quantigene TA assay probe set design: Label Extenders (LE) hybridize to the capped primer sequence derived from provided synthetic RNA substrate and first bases of the de novo synthesized viral mRNA at the 5′-end (LE1), and to the poly a tail at the 3′-end (LE2). Capture Extenders (CE1-9) specifically hybridize to gene specific regions and concomitantly immobilize the captured RNA to the plate. Blocking Probes (BP) hybridize to different stretches of the de novo synthesized viral mRNA. The sequence shown in italics at the 3′-end was verified by 3′-RLM RACE (not complete sequence shown). The probe sets are supplied as a mix of all three by Panomics.
Accordingly, in a first embodiment, the present invention provides a compound having the general formula (IIa) or (IIb).
It is understood that throughout the present specification the term “a compound having the general formula (IIa) or (IIb)” encompasses pharmaceutically acceptable salts, solvates, polymorphs, prodrugs, codrugs, cocrystals, tautomers, racemates, enantiomers, or diastereomers or mixtures thereof unless mentioned otherwise.
A further embodiment of the present invention relates to a pharmaceutical composition comprising a compound having the general formula (IIa) or (IIb) and optionally one or more pharmaceutically acceptable excipient(s) and/or carrier(s).
The compounds having the general formula (IIa) or (IIb) are useful for treating, ameliorating or preventing viral diseases.
It has been surprisingly found that the compounds according to the present invention which have a specific bimetal binding fragment —C(═O)—C(OR21)═C—C(═O)— in combination with additional hydrophobic interactions by the specific CR28R29 group have improved properties. In particular, the interaction with protein could be optimized resulting in better binding properties. Additional interactions with relevant amino acids in the hydrophobic binding pocket of the protein could be established resulting in increasing enthalpic binding interactions with additional entropic factors by displacement of water molecules.
Before the present invention is described in detail below, it is to be understood that this invention is not limited to the particular methodology, protocols and reagents described herein as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims. Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art.
Preferably, the terms used herein are defined as described in “A multilingual glossary of biotechnological terms: (IUPAC Recommendations)”, Leuenberger, H. G. W, Nagel, B. and Kölbl, H. eds. (1995), Helvetica Chimica Acta, CH-4010 Basel, Switzerland.
Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise”, and variations such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps. In the following passages different aspects of the invention are defined in more detail. Each aspect so defined may be combined with any other aspect or aspects unless clearly indicated to the contrary. In particular, any feature indicated as being preferred or advantageous may be combined with any other feature or features indicated as being preferred or advantageous.
Several documents are cited throughout the text of this specification. Each of the documents cited herein (including all patents, patent applications, scientific publications, manufacturer's specifications, instructions, etc.), whether supra or infra, are hereby incorporated by reference in their entirety. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention.
The term “alkyl” refers to a saturated straight or branched carbon chain.
The term “aryl” preferably refers to a mono- or polycyclic aromatic compound having 5 to 20 carbon atoms, more preferably an aromatic monocyclic ring containing 6 carbon atoms, an aromatic bicyclic ring system containing 10 carbon atoms or an aromatic tricyclic ring system containing 14 carbon atoms. Examples are phenyl, naphthyl or anthracenyl, preferably phenyl.
The term “cycloalkyl” represents a cyclic version of “alkyl”. The term “cycloalkyl” is also meant to include bicyclic, tricyclic and polycyclic versions thereof. Unless specified otherwise, the cycloalkyl group can have 3 to 12 carbon atoms.
“Hal” or “halogen” represents F, Cl, Br and I.
The term “carbocyclyl” covers any five or six-membered hydrocarbon ring which does not include heteroatoms in the ring. The term “carbocyclyl ring” covers saturated (including cycloalkyl rings), unsaturated rings and aromatic rings (including aryl rings).
The term “heteroaryl” preferably refers to a five- or six-membered aromatic ring wherein one or more of the carbon atoms in the ring have been replaced by 1, 2, 3, or 4 (for the five-membered ring) or 1, 2, 3, 4, or 5 (for the six-membered ring) of the same or different heteroatoms, whereby the heteroatoms are selected from O, N and S. Examples of the heteroaryl group include pyrrole, pyrrolidine, oxolane, furan, imidazolidine, imidazole, pyrazole, oxazolidine, oxazole, thiazole, piperidine, pyridine, morpholine, piperazine, and dioxolane.
The term “heterocyclyl” covers any five or six-membered ring wherein at least one of the carbon atoms in the ring has been replaced by 1, 2, 3, or 4 (for the five membered ring) or 1, 2, 3, 4, or 5 (for the six membered ring) of the same or different heteroatoms, whereby the heteroatoms are selected from O, N and S. The term “heterocyclyl ring” covers saturated, unsaturated rings and aromatic rings (including heteroaryl rings). Examples include pyrrole, pyrrolidine, oxolane, furan, imidazolidine, imidazole, pyrazole, oxazolidine, oxazole, thiazole, piperidine, pyridine, morpholine, piperazine, and dioxolane.
The term “hydrocarbon group which contains from 5 to 20 carbon atoms and optionally 1 to 4 heteroatoms selected from O, N and S and which contains at least one ring” refers to any group having 5 to 20 carbon atoms and optionally 1 to 4 heteroatoms selected from O, N and 2 as long as the group contains at least one ring. The term is also meant to include bicyclic, tricyclic and polycyclic versions thereof. If more than one ring is present, they can be separate from each other or be annelated. The ring(s) can be either carbocyclic or heterocyclic and can be saturated, unsaturated or aromatic. The carbon atoms and heteroatoms can either all be present in the one or more rings or some of the carbon atoms and/or heteroatoms can be present outside of the ring, e.g., in a linker group (such as —(CH2)p— with p=1 to 6). Examples of these groups include -(optionally substituted C3-9 cycloalkyl), -(optionally substituted aryl) wherein the aryl group can be, for example, phenyl, -(optionally substituted biphenyl), adamantyl, —(C3-9 cycloalkyl)-aryl as well as the corresponding compounds with a linker.
If a compound or moiety is referred to as being “optionally substituted”, it can in each instance include 1 or more of the indicated substituents, whereby the substituents can be the same or different.
The term “pharmaceutically acceptable salt” refers to a salt of a compound of the present invention. Suitable pharmaceutically acceptable salts include acid addition salts which may, for example, be formed by mixing a solution of compounds of the present invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulfuric acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid. Furthermore, where the compound carries an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts (e.g., sodium or potassium salts); alkaline earth metal salts (e.g., calcium or magnesium salts); and salts formed with suitable organic ligands (e.g., ammonium, quaternary ammonium and amine cations formed using counteranions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl sulfonate and aryl sulfonate). Illustrative examples of pharmaceutically acceptable salts include, but are not limited to, acetate, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, butyrate, calcium edetate, camphorate, cam phorsulfonate, camsylate, carbonate, chloride, citrate, clavulanate, cyclopentanepropionate, digluconate, dihydrochloride, dodecylsulfate, edetate, edisylate, estolate, esylate, ethanesulfonate, formate, fumarate, gluceptate, glucoheptonate, gluconate, glutamate, glycerophosphate, glycolylarsanilate, hemisulfate, heptanoate, hexanoate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroiodide, 2-hydroxy-ethanesulfonate, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, lauryl sulfate, malate, maleate, malonate, mandelate, mesylate, methanesulfonate, methylsulfate, mucate, 2-naphthalenesulfonate, napsylate, nicotinate, nitrate, N-methylglucamine ammonium salt, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, pectinate, persulfate, 3-phenylpropionate, phosphate/diphosphate, picrate, pivalate, polygalacturonate, propionate, salicylate, stearate, sulfate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide, undecanoate, valerate, and the like (see, for example, S. M. Berge et al., “Pharmaceutical Salts”, J. Pharm. Sci., 66, pp. 1-19 (1977)).
When the compounds of the present invention are provided in crystalline form, the structure can contain solvent molecules. The solvents are typically pharmaceutically acceptable solvents and include, among others, water (hydrates) or organic solvents. Examples of possible solvates include ethanolates and iso-propanolates.
The term “codrug” refers to two or more therapeutic compounds bonded via a covalent chemical bond. A detailed definition can be found, e.g., in N. Das et al., European Journal of Pharmaceutical Sciences, 41, 2010, 571-588.
The term “cocrystal” refers to a multiple component crystal in which all components are solid under ambient conditions when in their pure form. These components co-exist as a stoichiometric or non-stoichometric ratio of a target molecule or ion (i.e., compound of the present invention) and one or more neutral molecular cocrystal formers. A detailed discussion can be found, for example, in Ning Shan et al., Drug Discovery Today, 13(9/10), 2008, 440-446 and in D. J. Good et al., Cryst. Growth Des., 9(5), 2009, 2252-2264.
The compounds of the present invention can also be provided in the form of a prodrug, namely a compound which is metabolized in vivo to the active metabolite. Suitable prodrugs are, for instance, esters, ethers, phosphonates, and carbonates. A detailed discussion of potential prodrugs can be found in J. Rautio (Ed.), Prodrugs and Targeted Delivery, Wiley-VCH, 2011, ISBN: 978-3-527-32603-7. More specific examples of suitable groups are given, among others, in US 2007/0072831 in paragraphs [0082] to [0118] under the headings prodrugs and protecting groups. Preferred examples of the prodrug include compounds in which R21—C(O)—R, —C(O)—OR, —PO(ORA)(ORB) or —OC(O)OR, in which R, RA and RB are independently selected from C1-6 alkyl, aryl, or heteroaryl, whereby the alkyl, aryl, or heteroaryl can be optionally substituted, e.g., by —OH or O—C1-6alkyl. Examples of R include C1-6 alkyl (CH3, t-butyl), phenyl, phenyl-OH or phenyl-OCH3.
The present invention provides a compound having the general formula (IIa) or (IIb).
The present invention provides a compound having the general formula (IIa) or (IIb) in which the following definitions apply.
R21 is selected from —H, -(optionally substituted C1-6 alkyl), —(CH2)q-(optionally substituted carbocyclyl), —(CH2)q-(optionally substituted heterocyclyl), and —C(O)—H, —C(O)-(optionally substituted C1-6 alkyl), —C(O)—(CH2)q-(optionally substituted carbocyclyl), —C(O)—(CH2)q-(optionally substituted heterocyclyl); preferably R21 is selected from —H, —C1-6 alkyl and —(CH2)q-(optionally substituted phenyl); more preferably R21 is selected from —H, —C1-6 alkyl and —(CH2)q-(phenyl).
R22 is selected from —H, -(optionally substituted C1-6 alkyl), —(CH2)q-(optionally substituted carbocyclyl), —(CH2)q-(optionally substituted heterocyclyl), —(CH2)p—OR25, and —(CH2)p—NR26R27; preferably R22 is selected from —H and —C1-6 alkyl.
R23 is selected from —R24 and —X21R24; preferably R23 is selected from —H, —C1-6 alkyl, and (CR*2)m-phenyl, wherein the phenyl ring can be optionally substituted with one or more substituents selected from —H, —C1-6 alkyl, —CF3, -halogen, —CN, —OH, and —O—C1-6 alkyl; more preferably R23 is selected from —H, —C1-6 alkyl, and —(CR*2)m-phenyl.
R24 is selected from —H and -(optionally substituted hydrocarbon group which contains from 1 to 20 carbon atoms and optionally 1 to 4 heteroatoms selected from O, N and S). In a preferred embodiment, R24 is selected from
wherein
X is absent, CH2, NH, C(O)NH, S or O;
R is independently selected from —H, —C1-6 alkyl, —CF3, -halogen, —CN, —OH, and —O—C1-6 alkyl,
wherein it is to be understood that there may be one or more substituents R in each of the above examples.
R25 is selected from —H, —C1-6 alkyl, and —(CH2CH2O)rH.
R26 is selected from —H, -(optionally substituted C1-6 alkyl), -(optionally substituted C3-9 carbocyclyl), —C1-4 alkyl-(optionally substituted C3-9 carbocyclyl), -(optionally substituted heterocyclyl having 3 to 7 ring atoms), and —C1-4 alkyl-(optionally substituted heterocyclyl having 3 to 7 ring atoms).
R27 is selected from —H, -(optionally substituted C1-6 alkyl), -(optionally substituted C3-9 carbocyclyl), —C1-4 alkyl-(optionally substituted C3-9 carbocyclyl), -(optionally substituted heterocyclyl having 3 to 7 ring atoms), and —C1-4 alkyl-(optionally substituted heterocyclyl having 3 to 7 ring atoms).
R28 is selected from —H and —C1-6 alkyl; preferably R28 is —H.
R29 is selected from —R34 and —X31R34; preferably R29 is selected from —H, —C1-6 alkyl, and —(CR*2)m-phenyl, wherein the phenyl ring can be optionally substituted with one or more substituents selected from —H, —C1-6 alkyl, —CF3, -halogen, —CN, —OH, and —O—C1-6 alkyl; more preferably R29 is selected from —H, —C1-6 alkyl, and —(CR*2)m-phenyl.
R34 is selected from —H and -(optionally substituted hydrocarbon group which contains from 1 to 20 carbon atoms and optionally 1 to 4 heteroatoms selected from O, N and S). In a preferred embodiment, R34 is selected from
wherein
X is absent, CH2, NH, C(O)NH, S or O;
R is independently selected from —H, —C1-6 alkyl, —CF3, -halogen, —CN, —OH, and —O—C1-6 alkyl,
wherein it is to be understood that there may be one or more substituents R in each of the above examples).
R* is selected from —H, a —C1-6 alkyl group, or a —C1-6 alkyl group which is substituted by one or more halogen atoms; more preferably R* is H.
R** is selected from —H and —C1-6 alkyl.
R*** is selected from —H and —C1-6 alkyl.
X21 is selected from (CR*2)m, NR26, N(R26)C(O), C(O)NR26, O, C(O), C(O)O, OC(O); N(R26)SO2, SO2N(R26), N(R26)SO2N(R26), S, SO, and SO2; preferably X21 is selected from (CR*2)m, NR26, N(R26)C(O), C(O)NR26, O, C(O), C(O)O, OC(O); more preferably X21 is (CR*2)m.
X22 is selected from NR26, N(R26)C(O), C(O)NR26, O, C(O), C(O)O, OC(O); N(R26)SO2, SO2N(R26), S, SO, and SO2.
X31 is selected from (CR*2)m, NR26, N(R26)C(O), C(O)NR26, O, C(O), C(O)O, OC(O); N(R26)SO2, SO2N(R26), N(R26)SO2N(R26), S, SO, and SO2; preferably X31 is selected from (CR*2)m, NR26, N(R26)C(O), C(O)NR26, O, C(O), C(O)O, OC(O); more preferably X31 is (CR*2)m.
m is 1 to 6; preferably m is 1 to 4.
p is 1 to 4.
q is 0 to 4.
r is 1 to 3.
s is 0 to 4.
The alkyl group can be optionally substituted with one or more substituents selected from halogen, —CN, —NR26R27, —OH, and —O—C1-6 alkyl.
The hydrocarbon group, heterocyclyl group, and/or carbocyclyl group can be optionally substituted with one or more substituents selected from halogen, —CN, —CF3, —CN, —(CH2)s—X22—R**, —C1-6 alkyl, —C3-9 carbocyclyl, —C1-4 alkyl-C3-9 carbocyclyl, -(heterocyclyl having 3 to 7 ring atoms), and —C1-4 alkyl-(heterocyclyl having 3 to 7 ring atoms); preferably halogen, —CN, —NR56R57, —OH, and —O—C1-6 alkyl.
It has been surprisingly found that the compounds according to the present invention which have a specific bimetal binding fragment —C(═O)—C(OR21)═C—C(═O)— in combination with additional hydrophobic interactions by the specific CR28R29 group have improved properties. Without wishing to be bound by theory it is assumed that the viral polymerase protein has a pocket for binding and that the moiety CR28R29 of the compounds of the present invention fills this pocket to a larger extent. It is further assumed that the larger moiety CR28R29 is able to provide more hydrophobic interaction with the pocket than smaller moieties such as methyl.
In particular, the interaction with protein could be optimized resulting in better binding properties. Additional interactions with relevant amino acids in the hydrophobic binding pocket of the protein could be established resulting in increasing enthalpic binding interactions with additional entropic factors by displacement of water molecules.
The compounds of the present invention can be administered to a patient in the form of a pharmaceutical composition which can optionally comprise one or more pharmaceutically acceptable excipient(s) and/or carrier(s).
The compounds of the present invention can be administered by various well known routes, including oral, rectal, intragastrical, intracranial and parenteral administration, e.g. intravenous, intramuscular, intranasal, intradermal, subcutaneous, and similar administration routes. Oral, intranasal and parenteral administration are particularly preferred. Depending on the route of administration different pharmaceutical formulations are required and some of those may require that protective coatings are applied to the drug formulation to prevent degradation of a compound of the invention in, for example, the digestive tract.
Thus, preferably, a compound of the invention is formulated as a syrup, an infusion or injection solution, a spray, a tablet, a capsule, a capslet, lozenge, a liposome, a suppository, a plaster, a band-aid, a retard capsule, a powder, or a slow release formulation. Preferably, the diluent is water, a buffer, a buffered salt solution or a salt solution and the carrier preferably is selected from the group consisting of cocoa butter and vitebesole.
Particular preferred pharmaceutical forms for the administration of a compound of the invention are forms suitable for injectionable use and include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases the final solution or dispersion form must be sterile and fluid. Typically, such a solution or dispersion will include a solvent or dispersion medium, containing, for example, water-buffered aqueous solutions, e.g. biocompatible buffers, ethanol, polyol, such as glycerol, propylene glycol, polyethylene glycol, suitable mixtures thereof, surfactants or vegetable oils. A compound of the invention can also be formulated into liposomes, in particular for parenteral administration. Liposomes provide the advantage of increased half life in the circulation, if compared to the free drug and a prolonged more even release of the enclosed drug.
Sterilization of infusion or injection solutions can be accomplished by any number of art recognized techniques including but not limited to addition of preservatives like anti-bacterial or anti-fungal agents, e.g. parabene, chlorobutanol, phenol, sorbic acid or thimersal. Further, isotonic agents, such as sugars or salts, in particular sodium chloride, may be incorporated in infusion or injection solutions.
Production of sterile injectable solutions containing one or several of the compounds of the invention is accomplished by incorporating the respective compound in the required amount in the appropriate solvent with various ingredients enumerated above as required followed by sterilization. To obtain a sterile powder the above solutions are vacuum-dried or freeze-dried as necessary. Preferred diluents of the present invention are water, physiological acceptable buffers, physiological acceptable buffer salt solutions or salt solutions. Preferred carriers are cocoa butter and vitebesole. Excipients which can be used with the various pharmaceutical forms of a compound of the invention can be chosen from the following non-limiting list:
In one embodiment the formulation is for oral administration and the formulation comprises one or more or all of the following ingredients: pregelatinized starch, talc, povidone K 30, croscarmellose sodium, sodium stearyl fumarate, gelatin, titanium dioxide, sorbitol, monosodium citrate, xanthan gum, titanium dioxide, flavoring, sodium benzoate and saccharin sodium.
If a compound of the invention is administered intranasally in a preferred embodiment, it may be administered in the form of a dry powder inhaler or an aerosol spray from a pressurized container, pump, spray or nebulizer with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, a hydrofluoro-alkane such as 1,1,1,2-tetrafluoroethane (HFA 134A™) or 1,1,1,2,3,3,3-heptafluoropropane (HFA 227EA™), carbon dioxide, or another suitable gas. The pressurized container, pump, spray or nebulizer may contain a solution or suspension of the compound of the invention, e.g., using a mixture of ethanol and the propellant as the solvent, which may additionally contain a lubricant, e.g., sorbitan trioleate.
Other suitable excipients can be found in the Handbook of Pharmaceutical Excipients, published by the American Pharmaceutical Association, which is herein incorporated by reference.
It is to be understood that depending on the severity of the disorder and the particular type which is treatable with one of the compounds of the invention, as well as on the respective patient to be treated, e.g. the general health status of the patient, etc., different doses of the respective compound are required to elicit a therapeutic or prophylactic effect. The determination of the appropriate dose lies within the discretion of the attending physician. It is contemplated that the dosage of a compound of the invention in the therapeutic or prophylactic use of the invention should be in the range of about 0.1 mg to about 1 g of the active ingredient (i.e. compound of the invention) per kg body weight. However, in a preferred use of the present invention a compound of the invention is administered to a subject in need thereof in an amount ranging from 1.0 to 500 mg/kg body weight, preferably ranging from 1 to 200 mg/kg body weight. The duration of therapy with a compound of the invention will vary, depending on the severity of the disease being treated and the condition and idiosyncratic response of each individual patient. In one preferred embodiment of a prophylactic or therapeutic use, from 10 mg to 200 mg of the compound are orally administered to an adult per day, depending on the severity of the disease and/or the degree of exposure to disease carriers.
As is known in the art, the pharmaceutically effective amount of a given composition will also depend on the administration route. In general, the required amount will be higher if the administration is through the gastrointestinal tract, e.g., by suppository, rectal, or by an intragastric probe, and lower if the route of administration is parenteral, e.g., intravenous. Typically, a compound of the invention will be administered in ranges of 50 mg to 1 g/kg body weight, preferably 10 mg to 500 mg/kg body weight, if rectal or intragastric administration is used and in ranges of 1 to 100 mg/kg body weight if parenteral administration is used. For intranasal administration, 1 to 100 mg/kg body weight are envisaged.
If a person is known to be at risk of developing a disease treatable with a compound of the invention, prophylactic administration of the biologically active blood serum or the pharmaceutical composition according to the invention may be possible. In these cases the respective compound of the invention is preferably administered in above outlined preferred and particular preferred doses on a daily basis. Preferably, from 0.1 mg to 1 g/kg body weight once a day, preferably 10 to 200 mg/kg body weight. This administration can be continued until the risk of developing the respective viral disorder has lessened. In most instances, however, a compound of the invention will be administered once a disease/disorder has been diagnosed. In these cases it is preferred that a first dose of a compound of the invention is administered one, two, three or four times daily.
The compounds of the present invention are particularly useful for treating, ameliorating, or preventing viral diseases. The type of viral disease is not particularly limited. Examples of possible viral diseases include, but are not limited to, viral diseases which are caused by Poxviridae, Herpesviridae, Adenoviridae, Papillomaviridae, Polyomaviridae, Parvoviridae, Hepadnaviridae, Reoviridae, Filoviridae, Paramyxoviridae, Rhabdoviridae, Orthomyxoviridae, Bunyaviridae, Arenaviridae, Coronaviridae, Picornaviridae, Hepeviridae, Caliciviridae, Astroviridae, Togaviridae, Flaviviridae, Deltavirus, Bornaviridae, and prions. Preferably viral diseases which are caused by Herpesviridae, Filoviridae, Paramyxoviridae, Rhabdoviridae, Orthomyxoviridae, Bunyaviridae, Arenaviridae, Coronaviridae, Picornaviridae, Togaviridae, Flaviviridae, more preferably viral diseases which are caused by orthomyxoviridae.
Examples of the various viruses are given in the following table.
Preferably, the compounds of the present invention are employed to treat influenza. The present invention covers all virus genera belonging to the family of orthomyxoviridae, specifically influenza virus type A, B, and C, isavirus, and thogotovirus. Within the present invention, the term “influenza” includes influenza caused by any influenza virus such as influenza virus type A, B, and C including their various stains and isolates, and also covers influenza A virus strains commonly referred to as bird flu and swine flu. The subject to be treated is not particularly restricted and can be any vertebrate, such as birds and mammals (including humans).
Without wishing to be bound by theory it is assumed that the compounds of the present invention are capable of inhibiting endonuclease activity, particularly that of influenza virus. More specifically it is assumed that they directly interfere with the N-terminal part of the influenza virus PA protein, which harbors endonuclease activity and is essential for influenza virus replication. Influenza virus replication takes place inside the cell within the nucleus. Thus, compounds designed to inhibit PA endonuclease activity need to cross both the cellular and the nuclear membrane, a property which strongly depends on designed-in physico-chemical properties of the compounds. The present invention shows that the claimed compounds have in vitro endonuclease inhibitory activity and have antiviral activity in vitro in cell-based assays.
A possible measure of the in vitro endonuclease inhibitory activity of the compounds having the formula (IIa) or (IIb) is the FRET (fluorescence-resonance energy transfer)-based endonuclease activity assay disclosed herein. Preferably, the compounds exhibit a % reduction of at least about 50% at 25 μM in the FRET assay. In this context, the % reduction is the % reduction of the initial reaction velocity (v0) measured as fluorescence increase of a dual-labelled RNA substrate cleaved by the influenza virus endonuclease subunit (PA-Nter) upon compound treatment compared to untreated samples. Preferably, the compounds exhibit an IC50 of less than about 50 μM, more preferably less than about 20 μM, in this assay. The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a compound in inhibiting biological or biochemical function and was calculated from the initial reaction velocities (v0) in a given concentration series ranging from maximum 100 μM to at least 2 nM.
The compounds having the general formula (IIa) or (IIb) can be used in combination with one or more other medicaments. The type of the other medicaments is not particularly limited and will depend on the disorder to be treated. Preferably, the other medicament will be a further medicament which is useful in treating, ameliorating or preventing a viral disease, more preferably a further medicament which is useful in treating, ameliorating or preventing influenza that has been caused by influenza virus infection and conditions associated with this viral infection such as viral pneumonia or secondary bacterial pneumonia and medicaments to treat symptoms such as chills, fever, sore throat, muscle pains, severe headache, coughing, weakness and fatigue. Furthermore, the compounds having the general formula (IIa) or (IIb) can be used in combination with anti-inflammatories.
The following combinations of medicaments are envisaged as being particularly suitable:
Various modifications and variations of the invention will be apparent to those skilled in the art without departing from the scope of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention which are obvious to those skilled in the relevant fields are intended to be covered by the present invention.
The following examples are merely illustrative of the present invention and should not be construed to limit the scope of the invention as indicated by the appended claims in any way.
The influenza A virus (IAV) PA-Nter fragment (amino acids 1-209) harboring the influenza endonuclease activity was generated and purified as described in Dias et al., Nature 2009; Apr. 16; 458(7240), 914-918. The protein was dissolved in buffer containing 20 mM Tris pH 8.0, 100 mM NaCl and 10 mM β-mercaptoethanol and aliquots were stored at −20° C.
A 20 bases dual-labelled RNA oligo with 5′-FAM fluorophore and 3′-BHQ1 quencher was used as a substrate to be cleaved by the endonuclease activity of the PA-Nter. Cleavage of the RNA substrate frees the fluorophore from the quencher resulting in an increase of the fluorescent signal.
All assay components were diluted in assay buffer containing 20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM MnCl2, 10 mM MgCl2 and 10 mM β-mercaptoethanol. The final concentration of PA-Nter was 0.5 μM and 1.6 μM RNA substrate. The test compounds were dissolved in DMSO and generally tested at two concentrations or a concentration series resulting in a final plate well DMSO concentration of 0.5%. In those cases where the compounds were not soluble at that concentration, they were tested at the highest soluble concentration.
5 μl of each compound dilution was provided in the wells of white 384-well microtiter plates (PerkinElmer) in eight replicates. After addition of PA-Nter dilution, the plates were sealed and incubated for 30 min at room temperature prior to the addition of 1.6 μM RNA substrate diluted in assay buffer. Subsequently, the increasing fluorescence signal of cleaved RNA was measured in a microplate reader (Synergy HT, Biotek) at 485 nm excitation and 535 nm emission wavelength. The kinetic read interval was 35 sec at a sensitivity of 35. Fluorescence signal data over a period of 20 min were used to calculate the initial velocity (v0) of substrate cleavage. Final readout was the % reduction of v0 of compound-treated samples compared to untreated. The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a compound in inhibiting biological or biochemical function and was calculated from the initial reaction velocities (v0) in a given concentration series ranging from maximum 100 μM to at least 2 nM.
The influenza A virus (IAV) was obtained from American Tissue Culture Collection (A/Aichi/2/68 (H3N2); VR-547). Virus stocks were prepared by propagation of virus on Mardin-Darby canine kidney (MDCK; ATCC CCL-34) cells and infectious titres of virus stocks were determined by the 50% tissue culture infective dose (TCID50) analysis as described in Reed, L. J., and H. Muench. 1938, Am. J. Hyg. 27:493-497.
MDCK cells were seeded in 96-well plates at 2×104 cells/well using DMEM/Ham's F-12 (1:1) medium containing 10% foetal bovine serum (FBS), 2 mM L-glutamine and 1% antibiotics (all from PAA). Until infection the cells were incubated for 5 hrs at 37° C., 5.0% CO2 to form a ˜80% confluent monolayer on the bottom of the well. Each test compound was dissolved in DMSO and generally tested at 25 μM and 250 μM. In those cases where the compounds were not soluble at that concentration they were tested at the highest soluble concentration. The compounds were diluted in infection medium (DMEM/Ham's F-12 (1:1) containing 5 μg/ml trypsin, and 1% antibiotics) for a final plate well DMSO concentration of 1%. The virus stock was diluted in infection medium (DMEM/Ham's F-12 (1:1) containing 5 μg/ml Trypsin, 1% DMSO, and 1% antibiotics) to a theoretical multiplicity of infection (MOI) of 0.05.
After removal of the culture medium and one washing step with PBS, virus and compound were added together to the cells. In the wells used for cytotoxicity determination (i.e. in the absence of viral infection), no virus suspension was added. Instead, infection medium was added. Each treatment was conducted in two replicates. After incubation at 37° C., 5% CO2 for 48 hrs, each well was observed microscopically for apparent cytotoxicity, precipitate formation, or other notable abnormalities. Then, cell viability was determined using CellTiter-Glo luminescent cell viability assay (Promega). The supernatant was removed carefully and 65 μl of the reconstituted reagent were added to each well and incubated with gentle shaking for 15 min at room temperature. Then, 60 μl of the solution was transferred to an opaque plate and luminescence (RLU) was measured using Synergy HT plate reader (Biotek).
Relative cell viability values of uninfected-treated versus uninfected-untreated cells were used to evaluate cytotoxicity of the compounds. Substances with a relative viability below 80% at the tested concentration were regarded as cytotoxic and retested at lower concentrations.
Reduction in the virus-mediated cytopathic effect (CPE) upon treatment with the compounds was calculated as follows: The response (RLU) of infected-untreated samples was subtracted from the response (RLU) of the infected-treated samples and then normalized to the viability of the corresponding uninfected sample resulting in % CPE reduction. The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a compound in inhibiting biological or biochemical function and was calculated from the RLU response in a given concentration series ranging from maximum 100 μM to at least 100 nM.
To analyze the activity of the inhibitors, a transcription assay (TA) was employed using the whole RNP complex in a cell-free environment without the use of radioactively labeled nucleotides.
An in vitro synthesized capped mRNA oligo serves as primer for viral mRNA synthesis as cap-snatching substrate for the viral RNPs and newly synthesized viral mRNA is detected using Quantigene® 2.0 technology. The Quantigene® (QG) technology is based on RNA hybridization bound to coated 96-well plates followed by branched DNA (bDNA) signal amplification. Three different types of probes are responsible for specific hybridization to the gene of interest. The Capture Extenders (CE) hybridize to specific gene regions and concurrently immobilize the RNA to the QG Capture Plate. The Label Extenders (LE) also specifically hybridize to the gene of interest and provide a sequence for the signal amplification tree to be built up via sequential hybridization of preAmplifier (PreAmp), Amplifier (Amp) and alkaline phosphatase Label Probe. The signal is then detected by adding chemiluminescent substrate and using a microplate luminometer for the read out. The third probe blocks nonspecific interactions (Blocking Probe; BP). Generally, probe sets for IAV detection are designed to detect either the negative sense genomic vRNA or synthesized positive sense RNA (+RNA), without differentiating between cRNA or mRNA for translation. For the TA assay, the probe sets and the QG 2.0 protocol were adapted and modified to fit the purpose of a biochemical assay suitable for testing of antiviral compounds in a cell-free environment.
All compounds were dissolved in DMSO and stored at 4° C. All other reagents were obtained from Sigma-Aldrich if not stated otherwise.
The substrate RNA used was derived from in vitro transcribed RNA synthesized by T7 High
Yield RNA Synthesis Kit (New England BioLabs Inc.) generated according to the manufacturer's protocol but with extended incubation time of 16 hr. The RNA product was gel-purified using miRNeasy Mini Kit (Qiagen). The RNA was enzymatically capped using ScriptCap m7G Capping System (CellScript, Madison Wis.). The resulting capped RNA oligonucleotide (5′-m7GpppG-GGG AAU ACU CAA GCU AUG CAU CGC AUU AGG CAC GUC GAA GUA-3′; SEQ ID NO:1) served as primer for the influenza virus polymerase.
All experiments were done on IAV strain A/PR/8/34, amplified either in embryonated chicken eggs or obtained purified and concentrated from Charles River Laboratories. Egg-amplified virus was PEG-precipitated using 4% w/v PEG8000 in 2 mM Tris-HCl (pH 8.0) buffer containing 100 mM NaCl (4° C., 45 min) and centrifuged at 3600 g at 4° C. for 45 min. The pellet was suspended in a 10 mM Tris-HCl (pH 8.0) buffer containing 100 mM NaCl and 6% w/v sucrose and was then purified through a 30% w/v sucrose cushion (109,000 g, 120 min, 4° C.).
The RNP purification was performed as previously published with some modifications (Klumpp et al. 2001. Influenza virus endoribonuclease, p. 451-466, 342 ed.). The virus lyophilisate was solved in 1× lysis buffer (1% w/v Triton X-100, 1 mg/mL lysolecithin, 2.5 mM MgCl2, 100 mM KCl, 5 mM DTT, 2.5% v/v glycerol, 20 mM Tris-HCl (pH8.0), 20 U/mL RNase inhibitor) at a final virus protein concentration of 2 mg/mL and was then incubated for 60 minutes at 30° C. 3.3 mL of the resulting lysate was loaded onto a glycerol gradient (2 mL 70% v/v, 1.5 mL 50% v/v, 0.75 mL 40% v/v and 3.6 mL 33% v/v—buffered in 20 mM Tris-HCl, 50 mM NaCl, 5 mM DTT, 5 mM 2-mercaptoethanol). The gradients were spun in a Sorvall Ultra centrifuge, AH641 rotor, for 6 hours at 4° C. and 240,000 g. Fractions (0.5 mL) were collected from the top of the gradient. The fractions containing the RNP particles were pooled, further concentrated with 10 kD VivaSpin2 columns and stored at −20° C. The RNP concentration was determined by UV spectroscopy, using OD260 nm of 1.0=60 mg/mL RNP as conversion factor (Klumpp et al. 2001. Influenza virus endoribonuclease, p. 451-466, 342 ed.).
All types of viral RNA were analysed by Quantigene® using specific probe sets designed to detect either the negative sense genomic vRNA (−RNA; Cat. No. SF-10318), newly synthesized positive sense RNA (+RNA; Cat. No. SF-10049), or newly synthesized viral mRNA (TA assay; SF-10542) according to the manufacturer's instructions with the exception that all incubation steps during the Quantigene® procedure were done at 49° C.
For the standard reaction, 80 pM RNPs were incubated for 2 hrs at 30° C. with a dilution series of the inhibitors at 1% v/v final DMSO concentration in reaction buffer (55 mM Tris-HCl, 20 mM KCl, 1 mM MgCl2, 0.2% v/v Triton X-100, 0.25 U/μL RNaseOut, 12.5 mM NaCl, 1.25 mM DTT, 1.25 mM 2-mercaptoethanol, 12.5% v/v glycerol). Then 2 nM capped RNA substrate was added, followed by incubation for 2 hrs at 30° C. The reaction was terminated by incubation at 95° C. for 5 min.
For the detection of the synthesized mRNA the Quantigene® 2.0 (Panomics. 2007. QuantiGene 2.0 Reagent System. User Manual) was used with the probe sets specified. The probe sets consists of Capture Extenders (CE), Label Extenders (LE) and Blocking Probes (BP) and were generated by and supplied as a mix of all three by Affymetrix/Panomics. The probe sequences are represented in SEQ ID NOs: 5 to 20 and are also given in
The response values (relative luminescence units) were analyzed using GraphPad Prism to determine IC50 values and 95% confidence intervals using a 4-parameter logistic equation. Positive and negative controls were included to define top and bottom for fitting the curve.
De novo synthesized viral mRNA was generated by incubating purified RNPs with a capped RNA substrate of known sequence.
The Quantigene® probe set “TA assay” detects newly synthesized viral mRNA coding for nucleoprotein (NP), the Label Extenders (LE1 and LE2) specifically hybridize to the snatched cap sequence 5′-cap-GGGGGAAUACUCAAG-3′ (SEQ ID NO: 2) cleaved off from the 44-mer RNA substrate and to the polyA sequence, respectively. The Capture Extenders (CE1-9) specifically hybridize to regions within the coding region of the IAV NP gene. Probe set “+RNA” detects positive sense viral RNA coding for NP by specifically binding to more than 10 different regions within the gene. LE and CE of this probe set hybridize to regions between nucleotides 1 and 1540 (GenBank CY147505) and does not distinguish between viral mRNA and viral cRNA. The third probe set “−RNA” specifically hybridized to negative sense RNA (nsRNA), coding for the nonstructural protein (NS).
Employing the above described TA assay, IC50 values were determined for the compounds of the present invention.
To a solution of t-BuOH (75.48 g, 1.02 mol) and pyridine (80.58 g, 1.02 mol) in DCM (400 mL) was added methyl 2-chloro-2-oxoacetate (83.00 g, 0.68 mol) dropwise under ice bath. The solution was warmed to room temperature and continued to stir overnight. After the reaction was completed according to LCMS, the resultant was washed with water (100 mL×2) and 2N HCl (100 mL×2) successively. The organic phase was dried over NaSO4 and concentrated to give B-1 (98.00 g, 90%) as a colorless oil.
2-(Benzyloxy)acetyl chloride (90.00 g, 0.49 mol) was added dropwise to MeOH (225 mL) under ice bath. The resulting solution was warmed to room temperature and continued to stir overnight. After the reaction was completed according to LCMS, the solvent was evaporated to dryness to give B-2 (78.00 g, 89%) as a colorless oil.
A solution of B-1 (38.00 g, 0.22 mol) and B-2 (36.00 g, 0.20 mol) in THF (720 mL) was added dropwise to LDA (2.0 M in THF) (120 mL, 0.24 mol) under dry ice-acetone bath. The resulting solution was warmed to room temperature and continued to stir overnight. After the neutralization with 2 N HCl, the solution was evaporated to dryness to give B-3, which was used directly in the next step.
A mixture of B-3 (the crude product from last step), benzylhydrazine hydrochloride (31.60 g, 0.20 mol) and t-BuOK (78.40 g, 0.70 mol) in MeOH (2 L) was stirred at room temperature overnight. The mixture was quenched with sat. NH4Cl solution (1 L) and MeOH was removed under reduce pressure. The residue was extracted with EtOAc (200 mL×3). The organic phase was washed with brine (200 mL×3), dried over NaSO4 and concentrated. The residue was purified by column chromatography on silica gel (ethyl acetate/petroleum ether=1/20˜1/1) to give B-4-1 (15.00 g, 22% for two steps) as a yellow solid.
B-4-2 was synthesized in the same manner as B-4-1.
A mixture of B-4-1 (15.00 g, 44.38 mmol) and 2N NaOH (150 mL) in MeOH (150 mL) was stirred at room temperature overnight. The solvent was removed under reduce pressure and the residue was adjusted to pH=3˜4 with 2N HCl. The precipitate was collected by filtration and dried in vacuo to give B-5-1 (10.50 g, 73%) as a yellow solid.
B-5-2 was synthesized in the same manner as B-5-1.
A mixture of B-5-1 (4.50 g, 13.89 mmol), HOBt (2.25 g, 16.67 mmol), EDCl (3.20 g, 16.67 mmol), N-(2-chloroethyl)propan-2-amine hydrochloride (2.43 g, 15.28 mmol) and DIEA (5.38 g, 41.67 mmol) in DCM (90 mL) was stirred at room temperature overnight. The mixture was diluted with DCM (90 mL), washed with brine (50 mL×3), dried over NaSO4 and concentrated. The residue was purified by column chromatography on silica gel (ethyl acetate/petroleum ether=1/20˜2/1) to give B-6-1 (0.50 g, 9.2%) as a purple solid.
B-6-2, B-6-3 and B-6-4 were synthesized in the same manner as B-6-1.
A solution of (2-bromoethyl)benzene (1.00 g, 5.43 mmol) and hydrazine hydrate (85%, 5 mL) in EtOH (5 mL) was stirred at 60° C. for 1 h. The mixture was cooled to room temperature and then purified by reversed phase column chromatography. The fraction containing product was adjusted to pH=1-2 with 2 N HCl to form stable salt. The resultant was concentrated to give B-7 (0.30 g, 32%) as a white solid.
A mixture of 2-methyloxirane (1.00 g, 17.2 mmol) and propan-2-amine (3.0 g, 51.7 mmol) in MeOH (16 mL) was stirred at room temperature overnight. The mixture was concentrated to give the crude alcohol intermediate. To the solution of above intermediate in CHCl3 (16 mL) was added SOCl2 (10.26 g, 86.2 mmol) dropwise under ice bath. The solution was heated to reflux and stirred for 3 h. The solvent was removed and the residue was recrystallized in petroleum ether to give B-8-1 (2.10 g, 71% for two steps)) as a yellow solid.
B-8-2 was synthesized in the same manner as B-8-1
A mixture of B-6-1 (20 mg, 0.05 mmol) and Pd/C (10 mg, 10% Pd) in MeOH (2 mL) was stirred at room temperature for 2 h under H2 atmosphere. The Pd/C was removed by filtration and the filtrate was concentrated in vacuo. The residue was purified by Pre-HPLC to give E-001-01 (5 mg, 33%) as a white solid.
A mixture of B-6-3 (15 mg, 0.03 mmol) in TFA (1 mL) and dichloromethane (0.5 mL) was stirred at room temperature for 6 h. The resultant was concentrated in vacuo and purified by Pre-HPLC to give E-001-02 (4 mg, 33%) as a white solid.
E-001-01 was obtained as a white solid according to Method A.
Yield: 33%
Mp:
MS (ESI): 302 (M+H)+
1H NMR (CDCl3, 400 MHz):
δ 7.26-7.33 (m, 5H), 4.95 (s, 2H), 4.78-4.83 (m, 1H), 3.33 (s, 2H), 3.14 (s, 2H), 1.45 (d, J=6.4 Hz, 6H)
13C NMR (d6-DMSO, 400 MHz):
E-001-03 was obtained as a gray solid according to Method A.
Yield: 33%
Mp:
MS (ESI): 316 (M+H)+
1H NMR (d-DMSO, 400 MHz):
δ 7.18-7.30 (m, 5H), 4.58-4.66 (m, 1H), 3.77-3.90 (m, 2H), 3.39-3.43 (m, 2H), 3.18 (s, 1H), 2.80-2.90 (m, 3H), 1.08 (dd, J=18.4 Hz, 6.4 Hz, 6H)
13C NMR (d6-DMSO, 400 MHz):
E-002-04 was obtained as a yellow solid according to Method B.
Yield: 32%
Mp:
MS (ESI): 316 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.19-7.26 (m, 5H), 5.08 (d, J=15.6 Hz, 1H), 4.70-4.74 (m, 2H), 4.08-4.11 (m, 1H), 3.50 (dd, J=13.2 Hz, 3.6 Hz, 1H), 3.29 (dd, J=13.2 Hz, 1.6 Hz, 1H), 1.05 (dd, J=8.0 Hz, 7.2 Hz, 6H), 0.69 (d, J=6.4 Hz 3H)
13C NMR (d6-DMSO, 400 MHz):
E-001-02 was obtained as a yellow solid according to Method B.
Yield: 33%
Mp:
MS (ESI): 378 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.14-7.21 (m, 6H), 7.00 (d, J=7.2 Hz 2H), 6.89 (d, J=7.2 Hz 2H), 5.00 (s, 1H), 4.86 (s, 1H), 4.68 (d, J=15.2 Hz, 2H), 3.74 (d, J=10.8 Hz, 1H), 3.53 (d, J=12.8 Hz, 1H), 1.04 (d, J=3.6 Hz, 3H), 0.72 (s, 3H)
13C NMR (d6-DMSO, 400 MHz):
To a solution of sodium (3.68 g, 0.16 mol) in MeOH (200 mL) was added diethyl malonate (25.60 g, 0.16 mol) and ethyl 2-diazoacetate (9.12 g, 0.08 mol) quickly under ice-water bath. The solution was warmed to r.t. and continued to stir for 72 h. After cooling to 0° C., 5 N HCl solution (30 mL) was added and water (260 mL) was then added. The precipitate was collected by filtration and dried in vacuo to give E-2-01 (9.00 g, 56%) as a yellow solid.
To a solution of E-2-01 (1.00 g, 5.0 mmol) in 0.2 N NaOH solution (25 mL) was added benzyl bromide (0.86 g, 5.0 mmol) dropwise over 1 h at 70° C. The mixture was continued to stir at the same temperature for additional 1 h. After cooling to r.t., the precipitate was collected by filtration to give E-2-02 (1.10 g, 76%) as a white solid.
To a mixture of 2-phenylacetaldehyde (10.0 g, 83.3 mmol) and ZnI2 (1.30 g, 4.2 mmol) was added TMSCN (9.1 g, 91.6 mmol) dropwise at r.t. The mixture was continued to stir for 30 min. THF (20 mL) was then added and the resulting solution was added dropwise into a suspension of LiAlH4 (3.5 g, 91.6 mmol) in THF (180 mL) at 60° C. The mixture was continued to stir at the same temperature for 4 h. After cooling to 0° C., the mixture was diluted with THF (80 mL), water (3.5 mL) and 15% NaOH (3.5 mL). After stirring for 15 min, water (10.5 mL) and MgSO4 were added successively. The resulting mixture was continued to stir for 15 min. The precipitate was removed by filtration, and the filtrate was concentrated to give E-2-03-2 as a crude product.
To a solution of E-2-03-2 (the above crude product) in DCM (200 mL) was added (Boc)2O (18.1 g, 83.3 mmol) dropwise at 0° C. The solution was warmed to r.t. and continued to stir overnight. The solvent was removed under the reduce pressure and the residue was purified by column chromatography on silica gel (ethyl acetate/petroleum ether=1/100˜1/5) to give E-2-04-2 (3.0 g, 14% two step) as a yellow oil.
E-2-04-1 was synthesized in the same manner as E-2-04-2 (E-2-04-1 was commercially available).
To a solution of E-2-04-2 (2.00 g, 8.0 mmol) and TEA (1.21 g, 12.0 mmol) in DCM (40 mL) was added MsCl (0.91 g, 8.0 mmol) dropwise under ice-water bath. The reaction mixture was continued to stir at r.t. for 1 h. The mixture was diluted with DCM (5 mL), washed with brine (2 mL×3), dried over NaSO4 and concentrated to give E-2-05-2 as a crude product.
E-2-05-1 was synthesized in the same manner as E-2-05-2.
A mixture of E-2-02 (1.16 g, 4.0 mmol), E-2-05-2 (1.58 g, 4.8 mmol) and Cs2CO3 (1.96 g, 6.0 mmol) in DMF (23 mL) was stirred at 50° C. for overnight. The mixture was diluted with water (25 mL), extracted with EtOAc (20 mL×3). The combined organic phase was washed with brine (20 mL×3), dried over NaSO4 and concentrated. The residue was purified by column chromatography on silica gel (ethyl acetate/petroleum ether=1/20˜1/5) to give E-2-06-2 (1.60 g, 75%) as a yellow oil.
E-2-06-1 was synthesized in the same manner as E-2-06-2.
A solution of E-2-06-2 (1.40 g, 3.0 mmol) and TFA (3.5 mL) in DCM (14 mL) was stirred at r.t. for 2 h. The solution was concentrated to give E-2-07-2 as a crude product.
E-2-07-1 was synthesized in the same manner as E-2-07-2.
A solution of E-002-07-2 (crude product from last step) and TEA (1.52 g, 15.0 mmol) in MeOH (20 mL) was heated to reflux for 1 h. The solvent was removed under the reduce pressure and the residue was purified by column chromatography on silica gel (ethyl acetate/petroleum ether=1/20˜1/1) to give E-2-08-2 (0.5 g, 43% two step) as a white solid.
E-2-08-1 was synthesized in the same manner as E-2-08-2.
A mixture of E-2-08-2 (0.20 g, 0.5 mmol), MeI (0.15 g, 1.0 mol) and K2CO3 (0.21 g, 1.5 mol) in DMF (4 mL) was stirred at r.t. overnight. The mixture was diluted with water (10 mL) and extracted with EtOAc (10 mL×3). The combined organic phase was washed with brine (10 mL×3), dried over NaSO4 and concentrated. The residue was purified by Pre-TLC (ethyl acetate/petroleum ether=1/1) to give E-2-09-2 (60 mg, 29%) as a yellow solid.
E-2-09-1 was synthesized in the same manner as E-2-09-2.
A mixture of E-2-09-2 (60 mg, 0.15 mmol) and Pd/C (10 mg, 10% Pd) in MeOH/EtOAc (2.5 mL/1 mL) was stirred at r.t. for 1 h under H2 atmosphere. Pd/C was removed by filtration and the filtrate was concentrated in vacuo to give E-2-10-2 (30 mg, 63%) as a grey solid.
E-2-10-1 was synthesized in the same manner as E-2-10-2.
E-2-11-1 was synthesized in the same manner as E-2-10-2.
E-2-11-2 was synthesized in the same manner as E-2-10-2.
A mixture of E-2-10-2 (25 mg, 0.08 mmol) and 2N NaOH solution (1 mL) in MeOH (1 mL) was stirred at 40° C. for 2 h. The mixture was cooled to r.t. and adjusted to pH=3˜4 with 2N HCl. The resultant was extracted with EtOAc (5 mL×3). The combined organic phase was washed with brine (5 mL×3), dried over NaSO4 and concentrated to give E-2-12 (8 mg, 33%) as a white solid.
To a mixture of E-2-12-3 (60 mg, 0.15 mmol) and 2-iodopropane (51 mg, 0.3 mmol) in DMF (1 mL) was added NaH (12 mg, 0.3 mmol, 60%) under ice-water bath. The mixture was warmed to r.t. and continued to stir for 2 h. The reaction mixture was quenched with saturated NHCl4 (2 mL) and extracted with EtOAc (5 mL×3). The combined organic phase was washed with brine (5 mL×3), dried over NaSO4 and concentrated. The residue was purified by Pre-HPLC to give E-2-12-1 (20 mg, 31%) as a white solid.
A mixture of E-2-12-1 (10 mg, 0.024 mmol) and Pd/C (5 mg, 10% Pd) in MeOH (2 mL) was stirred at r.t. for 1 h under H2 atmosphere. Pd/C was removed by filtration and the filtrate was concentrated. The residue was purified by Pre-HPLC to give E-2-12-2 (3 mg, 38%) as a white solid.
E-2-12-3 was obtained as a white solid
Yield: 39% (two steps)
Mp:
MS (ESI): 302 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.49 (d, J=6.4 Hz, 2H), 7.32-7.35 (m, 3H), 5.30 (s, 2H), 4.36 (t, J=6.0 Hz, 2H), 3.89 (s, 3H), 3.70 (t, J=6.0 Hz, 2H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3A was obtained as a white solid
Yield: 70%
Mp:
MS (ESI): 212 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 4.22 (t, J=6.0 Hz, 2H), 3.80 (s, 3H), 3.60 (t, J=6.0 Hz, 2H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3B was obtained as a white solid
Yield: 26%
Mp:
MS (ESI): 288 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.39 (d, J=7.2 Hz, 2H), 7.18-7.23 (m, 3H), 5.21 (s, 2H), 4.23 (t, J=6.0 Hz, 2H), 3.57 (t, J=4.4 Hz, 2H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3C was obtained as a white solid
Yield: 21%
Mp:
MS (ESI): 198 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 4.32 (t, J=5.2 Hz, 2H), 3.75 (t, J=5.2 Hz, 2H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3D was obtained as a yellow solid
Yield: 19%
Mp:
MS (ESI): 316 (M+H)+
1H NMR (CDCl3, 400 MHz):
δ 7.48 (d, J=7.2 Hz, 2H), 7.23-7.30 (m, 3H), 5.29 (s, 2H), 4.33 (t, J=6.0 Hz, 2H), 3.86 (s, 3H), 3.67 (t, J=6.0 Hz, 2H), 3.10 (s, 3H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3F was obtained as a white solid
Yield: 21%
Mp:
MS (ESI): 212 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 4.27 (t, J=5.2 Hz, 2H), 3.71 (t, J=5.2 Hz, 2H), 2.99 (s, 3H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3G was obtained as a white solid
Yield: 43% (two steps)
Mp:
MS (ESI): 392 (M+H)+
1H NMR (CDCl3, 400 MHz):
δ 7.46 (d, J=6.8 Hz, 2H), 7.21-7.30 (m, 6H), 7.14-7.16 (m, 2H), 5.84 (s, 1H), 5.31-5.37 (m, 2H), 4.53-4.57 (m, 1H), 3.89 (s, 3H), 3.56-3.60 (m, 1H), 3.40 (dd, J=13.6 Hz, 4.4 Hz, 1H), 3.32 (dt, J=13.2 Hz, 4.0 Hz, 1H), 3.95 (dd, J=13.6 Hz, 11.2 Hz, 1H),
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3L was obtained as a white solid
Yield: 87%
Mp:
MS (ESI): 302 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.33-7.36 (m, 2H), 7.25-7.30 (m, 3H), 4.61-4.64 (m, 1H), 3.94 (s, 3H), 3.64 (dd, J=13.2 Hz, 4.4 Hz, 1H), 3.36-3.45 (m, 2H), 3.05 (dd, J=13.6 Hz, 10.0 Hz, 1H),
13C NMR (d6-DMSO, 400 MHz):
E-2-12-3M was obtained as a white solid
Yield: 70%
Mp:
MS (ESI): 288 (M+H)+
1H NMR (d6-DMSO, 400 MHz):
δ 12.97 (br, 1H), 8.95 (br, 1H), 8.00 (s, 1H), 7.32-7.36 (m, 2H), 7.25-7.30 (m, 3H), 4.57-4.62 (m, 1H), 3.42 (d, J=11.6 Hz, 1H), 3.23-3.31 (m, 1H), 2.94 (dd, J=13.6 Hz, 10.0 Hz, 1H), 2.53-2.56 (m, 1H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-4 was obtained as a yellow solid
Yield: 29%
Mp:
MS (ESI): 406 (M+H)+
1H NMR (CDCl3, 400 MHz):
δ 7.50 (d, J=5.6 Hz, 2H), 7.22-7.32 (m, 6H), 7.11 (d, J=7.2 Hz, 2H), 5.29 (d, J=2.8 Hz, 2H), 4.54-4.58 (m, 1H), 3.89 (s, 3H), 3.64 (dd, J=13.2 Hz, 4.4 Hz, 1H), 3.40 (dd, J=13.6 Hz, 4.0 Hz, 1H), 3.26 (dd, J=13.2 Hz, 3.6 Hz, 1H), 3.03 (s, 3H), 2.90 (dd, J=13.2 Hz, 10.8 Hz, 1H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-5 was obtained as a grey solid
Yield: 63%
Mp:
MS (ESI): 316 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.26-7.35 (m, 3H), 7.18 (d, J=7.6 Hz, 2H), 4.68-4.73 (m, 1H), 3.94 (s, 3H), 3.81 (dd, J=13.6 Hz, 4.84 Hz, 1H), 3.50 (dd, J=13.2 Hz, 4.4 Hz, 1H), 3.38 (dd, J=13.6 Hz, 4.4 Hz, 1H), 3.06 (dd, J=13.2 Hz, 8.8 Hz, 1H), 2.99 (s, 3H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12 was obtained as a white solid
Yield: 33%
Mp:
MS (ESI): 302 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.26-7.36 (m, 3H), 7.19 (d, J=7.6 Hz, 2H), 4.70-4.73 (m, 1H), 3.81 (dd, J=13.6 Hz, 4.4 Hz, 1H), 3.50 (dd, J=13.6 Hz, 4.4 Hz, 1H), 3.39 (dd, J=13.6 Hz, 4.8 Hz, 1H), 3.07 (dd, J=13.6 Hz, 4.8 Hz, 1H), 3.00 (s, 3H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-1 was obtained as a white solid
Yield: 31%
Mp:
MS (ESI): 420 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.51 (d, J=6.8 Hz, 2H), 7.28-7.36 (m, 6H), 7.20 (d, J=7.2 Hz, 2H), 5.31 (s, 2H), 4.88-4.92 (m, 1H), 4.73-4.77 (m, 1H), 3.65 (dd, J=13.2 Hz, 4.0 Hz, 1H), 3.47 (dd, J=13.6 Hz, 4.4 Hz, 1H), 3.35-3.36 (m, 1H), 3.06 (dd, J=13.6 Hz, 9.2 Hz, 1H), 1.22 (d, J=6.8 Hz, 3H), 1.15 (d, J=6.8 Hz, 3H)
13C NMR (d6-DMSO, 400 MHz):
E-2-12-2 was obtained as a white solid
Yield: 63%
Mp:
MS (ESI): 330 (M+H)+
1H NMR (CD3OD, 400 MHz):
δ 7.13-7.25 (m, 5H), 4.74-7.84 (m, 1H), 4.62-4.65 (m, 1H), 3.55 (dd, J=13.2 Hz, 3.2 Hz, 1H), 3.35 (dd, J=13.2 Hz, 3.6 Hz, 1H), 3.21-3.26 (m, 1H), 2.94 (dd, J=13.6 Hz, 9.6 Hz, 1H), 1.09 (d, J=6.8 Hz, 3H), 1.02 (d, J=6.8 Hz, 3H)
13C NMR (d6-DMSO, 400 MHz):
This application claims priority from U.S. Provisional Patent Application No. 62/220,821, filed Sep. 18, 2015, which is incorporated herein by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| 62220821 | Sep 2015 | US |
