Patent Application
20060135525
This invention relates to a novel pyrazolopyrimidine compound, which has a small-conductance potassium channel (SK) blocking activity and is useful as a medicament and a method for preparing the same.
Ca2+-activated potassium (K) channels consist of at least three subtypes: Big- (BK), Intermediate- (IK) and Small-conductance K channel. These channels are activated by increase in intracellular Ca2+ level. Although BK and IK channels are sensitive to changes in membrane voltage and increase in intracellular Ca2+ level, SK channels are not significantly sensitive to the change in membrane voltage. Besides, SK channels are characterized in that the channels have a low conductance of 6 to 20 pS to single channel and a higher sensitivity to apamin. SK channels are present not only in excitable cells such as nerve cells and muscle cells but also in other kinds of cells such as liver cells or blood cells, and may be responsible for various cell functions including chemokine release, muscle contraction and secretion.
Apamin is a well-known selective SK channel blocker, and it has been reported that this agent activates gastrointestinal peristaltic function (S. A. Waterman and M. Costa, J. Physiology 477, 459-468, 1994; N. Spencer et al., J. Physiology 517, 889-898, 1999), improves cognitive/acquisition deficits (S. Ikonen et al., Eur. J. Pharmacol. 347, 13-21, 1998; C. Ghelardini et al., Br. J. Pharmacol. 123, 1079-1084, 1998) and decreases immobility time in mouse forced swimming test (N. Galeotti et al., Br. J. Pharmacol. 126, 1653-1659, 1999). Moreover, it is reported that a specific receptor for apamin exists in skeletal muscle cells and administration of this agent alleviates the symptoms in patients with myotonic muscle dystrophy (J. F. Renaud et al., Nature 319, 678-680, 1986; M. L Behrens et al., Muscle & Nerve 17, 1264-1270, 1994). Furthermore, it was reported that mice showed abnormal respiratory responses to hypoxia under conditional overexpression of SK subtype (SK3) (C. T. Bond et al., Science 289, 1942-1946, 2000).
As compounds having a SK channel-blocking activity, bis(benzimidazol) compounds such as 1,1′-(α, α′-p-xylene)-3,3′-(α, α′-m-xylene)-bis(benzimidazolium), cyclophan compounds such as 7,18-diaza-3,4(1,4)-dibenzena-1,6(1,4)-diquinolin-acyclooctadecaphan 3 trifluoroacetate hydrate, cross-linked bisquinoline compounds such as 1,4-bis-(2-methyl-quinolin-4-yl)-[1,4]-diazepane and compounds having a cyclohexane-1,1′(2′H)-spiroisoquinoline moiety are disclosed in International Patent Publication WO00/01676, WO97/48705, the U.S. Pat. No. 5,866,562 and WO02/79189, respectively.
The object of the present invention is to provide a novel pyrazolopyrimidine compound as a medicament having an excellent SK channel blocking activity and a method for preparing the same.
The present invention relates to a pyrazolopyrimidine compound of the formula
wherein R1 is
(A) a substituted aryl group,
(B) an optionally substituted nitrogen-containing aliphatic heteromonocyclic group,
(C) a substituted cyclo-lower alkyl group,
(D) an optionally substituted amino group, or
(E) a substituted heteroaryl group,
R2 is (a) an optionally substituted heteroaryl group or (b) an optionally substituted aryl group,
Y is a single bond, a lower alkylene group or a lower alkenylene group,
Z is a group of the formula: —CO—, —CH2—, —SO2— or
Q is a lower alkylene group, and q is an integer of 0 or 1 or a pharmaceutically acceptable salt thereof
In case that R1 in the compound [I] is (A) a substituted aryl group, the substituent may be the same or different one to three groups selected from the group consisting of
(i) a hydroxyl group; (ii) a halogen atom; (iii) a lower alkyl group; (iv) an amino group optionally substituted by one or two groups selected from a lower alkyl group optionally substituted by a hydroxyl group, a lower alkoxy-lower alkyl group, an amino-lower alkanoyl group optionally substituted by a group selected from a lower alkyl group, a lower alkoxycarbonyl group and a group of the formula:
in which R31 is a lower alkyl group at the amino moiety, a (mono- or di-lower alkyl)amino-lower alkyl group, a (mono- or di-lower alkyl)carbamoyl group, a lower alkanoyl group optionally substituted by a hydroxyl group, a cyclo-lower alkylcarbonyl group, a lower alkoxy-lower alkanoyl group, a lower alkoxy-lower alkoxycarbonyl group, a cyclo-lower alkyl-lower alkyl group, a lower alkylsulfonyl group, an aryl-lower alkyl group optionally substituted by a (mono- or di-lower alkyl)amino group, a lower alkenoyl group, a thiocarbamoyl group optionally substituted by a lower alkyl group, a heteroarylcarbonyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkanoyl group, an arylsulfonyl group optionally substituted by a (mono- or di-lower alkyl)amino group at the aryl moiety; a group of the formula:
in which R32 is a lower alkoxy group and a nitrogen-containing aliphatic heteromonocyclic group optionally substituted by a lower alkyl group, (v) a lower alkoxy group optionally substituted by a group selected from an amino group optionally substituted by a group(s) selected from a lower alkyl group and an aryl-lower alkyl group, a heteroaryl group optionally substituted by a lower alkyl group and a nitrogen-containing aliphatic heteromonocyclic group optionally substituted by a lower alkyl group; (vi) an amino-lower alkyl group optionally substituted by a group selected from a lower alkyl group optionally substituted by a hydroxyl group, a lower alkanoyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkoxycarbonyl group, a lower alkoxy-lower alkanoyl group, a (mono- or di-lower alkyl)carbamoyl group, a lower alkoxy-lower alkoxycarbonyl group, a lower alkoxy-lower alkyl group, a cyclo-lower alkylcarbonyl group, an aryl-lower alkyl group, a cyclo-lower alkyl group, a cyclo-lower alkyl-lower alkyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkoxycarbonyl group and a group of the formula:
in which R33 is an amino group, a (mono- or di-lower alkyl)amino group or a (mono- or di-lower alkyl)amino-lower alkylamino group; (vii) a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group optionally substituted by a group selected from a hydroxyl group, a lower alkyl group optionally substituted by a hydroxyl group, a lower alkoxy-lower alkyl group and a carbamoyl group; (viii) a carbamoyl group optionally substituted by a group selected from a lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a heteroaryl group-substituted lower alkyl group and a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group; (ix) a nitrogen-containing aliphatic heteromonocyclic group optionally substituted by a lower alkyl group (said nitrogen-containing aliphatic heteromonocyclic group may bond to the aryl moiety via an oxygen atom); (x) a nitro group; (xi) a cyclo-lower alkyloxy group optionally substituted by a (mono- or di-lower alkyl)amino group; (xii) a lower alkenyl group optionally substituted by a group selected from a (mono- or di-lower alkyl)amino group and a nitrogen-containing aliphatic heteromonocyclic group; (xiii) a lower alkynyl group optionally substituted by a group(s) selected from a (mono- or di-lower alkyl)amino group and a nitrogen-containing aliphatic heteromonocyclic group; (xiv) a lower alkylthio group optionally substituted by a (mono- or di-lower alkyl)amino group; and (xv) a cyclo-lower alkyl-lower alkoxy group optionally substituted by a (mono- or di-lower alkyl)amino group at the cyclo-lower alkyl moiety.
In case that R1 in the compound [I] is (B) an optionally substituted nitrogen-containing aliphatic heteromonocyclic group, the substituent may be the group(s) selected from a lower alkyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkanoyl group, a (mono- or di-lower alkyl)amino-lower alkanoyl group, a lower alkoxy-lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a cyclo-lower alkyl group, a heteroaryl group, a nitrogen-containing aliphatic heteromonocyclic group optionally containing one or more double bond in the ring and optionally substituted by a group(s) selected from a lower alkyl group, a lower alkoxy-lower alkyl group, a carbamoyl group and a lower alkanoyl-amino group and an amino group optionally substituted by a group(s) selected from a lower alkyl group, a (mono- or di-lower alkyl)amino group, a cyclo-lower alkyl-carbonyl group, a lower alkenoyl group, a heteroarylcarbonyl group, a lower alkoxy-lower alkyl group, a lower alkanoyl group and a nitrogen-containing aliphatic heteromonocyclic group.
In case that R1 in the compound [I] is (C) a substituted cyclo-lower alkyl group, the substituent may be a group(s) selected from a group consisting of (i) an amino group optionally substituted by a group selected from a lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkanoyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkanoyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a lower alkanoyl group, a cyclo-lower alkylcarbonyl group, a lower alkenoyl group, a heteroarylcarbonyl group, an arylcarbonyl group optionally substituted by a halogen atom(s), a lower alkyl-thiocarbamoyl group, a lower alkoxycarbonyl group, a cyclo-lower alkyl group, a group of the formula:
in which R34 is a (mono- or di-lower alkyl)amino group, a cyclo-lower alkyl-lower alkyl group and a lower alkylsulfonyl group; (ii) an amino-lower alkyl group optionally substituted by a group selected from a lower alkyl group optionally substituted by a hydroxyl group, a (mono- or di-lower alkyl)amino-lower alkanoyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkanoyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a heteroaryl group-substituted lower alkyl group, a lower alkoxy-lower alkyl group, a lower alkanoyl group, a heteroarylcarbonyl group (the heteroaryl moiety of said group is optionally substituted by a lower alkyl group), a cyclo-lower alkylcarbonyl group, an aryl-lower alkyl group, a cyclo-lower alkyl group, a cyclo-lower alkyl-lower alkyl group, a lower alkylsulfonyl group, a lower alkoxycarbonyl group, a mono- or di-lower alkylcarbamoyl group and an arylcarbonyl group optionally substituted by a group(s) selected from a halogen atom and a lower alkoxy group, a lower alkoxy-lower alkanoyl group and a lower alkanoyl group; (iii) a nitrogen-containing aliphatic heteromonocyclic group optionally substituted by a group(s) selected from a hydroxyl group, a lower alkyl group, a lower alkanoyl group and a lower alkoxy-lower alkyl group; (iv) a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group (said nitrogen-containing aliphatic heteromonocyclic group is optionally fused to a benzene ring and optionally substituted by a group selected from a lower alkyl group, a carbamoyl (or thiocarbamoyl) group, a hydroxyl group, a lower alkoxy-lower alkyl group, a lower alkanoyl group and a (mono- or di-lower alkyl)amino group); (v) a mono- or di-lower alkylamino-lower alkoxy group; and (vi) a carbamoyl group optionally substituted by a group(s) selected from a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group optionally substituted by a lower alkyl group, a (mono- or di-lower alkyl)amino group and a lower alkyl group.
In case that R1 in the compound [II] is (D) an optionally substituted amino group, the substituent may be a lower alkyl group.
In case that R1 in the compound [I] is (E) a substituted heteroaryl group, the substituent may be a group(s) selected from (i) an amino-lower alkyl group optionally substituted by a group(s) selected from a lower alkyl group and a lower alkoxy-lower alkyl group; (ii) an amino group optionally substituted by a group selected from a cyclo-lower alkylcarbonyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a lower alkanoyl group, a lower alkenoyl group, a (mono- or di-lower alkyl)thiocarbamoyl group, a (mono- or di-lower alkyl)carbamoyl group and a lower alkyl group; (iii) a carbamoyl group optionally substituted by a group selected from a lower alkyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group and a (mono- or di-lower alkyl)amino-lower alkyl group; (iv) a lower alkyl group optionally substituted by a halogen atom(s); (v) a (mono- or di-lower alkyl)amino-lower alkoxy group; (vi) an oxo group; and (vii) a group of the formula:
wherein ring A is a nitrogen-containing aliphatic heteromonocyclic group optionally substituted by a lower alkyl group and optionally fused to a benzene ring, Ya is a single bond, a lower alkylene group or a lower alkenylene group and p is an integer of 0 or 1.
In case that R2 in the compound [I] is (a) an optionally substituted heteroaryl group, the substituent may be the same or different one to three groups selected from a lower alkyl group, a lower alkoxy group and a (mono- or di-lower alkyl)amino group. In case that R2 in the compound [I] is (b) an optionally substituted aryl group, the substituent may be the same or different one to three groups selected from a lower alkyl group, a halogen atom, a halogeno-lower alkoxy group, a (mono- or di-lower alkyl)amino group, a lower alkoxy group, a nitro group, a lower alkoxy-lower alkyl group, a hydroxyl group, a lower alkanoyl group and a lower alkoxycarbonyl group.
Examples of the aryl group in R1 and R2 include a 6- to 10-membered, mono- or bi-cyclic aryl group such as a phenyl group and a naphthyl group.
Examples of the nitrogen-containing aliphatic heteromonocyclic group in R1 and R2 include a 4- to 8-membered (preferably 5- or 6-membered) nitrogen-containing aliphatic heteromonocyclic group (said cyclic group optionally contain one or more double bond in the ring) such as an azetidinyl group, a pyrrolidinyl group, an imidazolidinyl group, a pyrazolidinyl group, a piperidyl group, a piperazinyl group, an azepinyl group, a diazepinyl group, an azeocinyl group, a diazeocinyl group, a 3-pyrrolinyl group or a morpholinyl group.
Examples of the heteroaryl group in R1 and R2 include a 5- to 14-membered (preferably 5- to 10-membered) mono- or bi-cyclic heteroaryl group having at least one heteroatom selected from nitrogen atom, sulfur atom and oxygen atom. Such heteroaryl group may be a nitrogen-containing heteroaryl group selected from a pyrrolyl group, an imidazolyl group, a pyrazolyl group, an oxazolyl group, a thiazolyl group, an isothiazolyl group, an isoxazolyl group, a pyridyl group, a dihydropyridyl group, a pyrazinyl group, a pyrimidinyl group, a tetrahydropyrimidinyl group, a furopyrimidinyl group, a pyridazinyl group, an imidazolidinyl group, an indolyl group, a quinolyl group, an isoquinolyl group, a purinyl group, a 1H-indazolyl group, a quinazolinyl group, a cinnolinyl group, a quinoxalinyl group, a phthalazinyl group and a pteridinyl group or an oxygen- or sulfur-containing heteroaryl group selected from a furyl group, a pyranyl group, a thienyl group, a benzofuryl group and a benzothienyl group.
Examples of the lower alkylene group and the lower alkenylene group in Y includes an alkylene group having one to eight carbon atoms (e.g., methylene group, ethylene group or propylene group) and an alkenylene group having two to eight carbon atoms (e.g., vinylene group or propenylene group), respectively.
Among the objective compounds [I] of the present invention, preferred examples are (1) those in which Y is a single bond, a lower alkylene group or a lower alkenylene group, Z is —CO—, R2 is a phenyl group substituted by a group selected from a lower alkyl group, a lower alkoxy group and a halogen atom, a lower alkoxy-substituted heteroaryl group or a lower alkyl-substituted heteroaryl group and q is an integer of 0, or (2) those in which Y is a single bond, Z is —CH2—, R2 is a lower alkoxyphenyl group and q is an integer of 0.
Among the objective compounds [I] of the present invention, more preferred examples are those in which R1 is (a) a phenyl group substituted by a group selected from (i) a lower alkoxy group substituted by a group selected from a (mono- or di-lower alkyl)amino group and a nitrogen-containing aliphatic heteromonocyclic group, (ii) a lower allyl group substituted by a group selected from a (mono- or di-lower alkyl)-amino group and a nitrogen-containing aliphatic heteromonocyclic group, and (iii) an amino group substituted by a group selected from a lower alkyl group, a cyclo-lower alkylcarbonyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a lower alkoxy-lower alkoxycarbonyl group, a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group, a lower alkanoyl group and a lower alkenoyl group, (b) a cyclo-lower alkyl group substituted by a group selected from (i) an amino-lower alkyl group optionally substituted by a group selected from a lower alkyl group, a hydroxy-lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a lower alkanoyl group, a cyclo-lower alkylcarbonyl group and a lower alkoxy-lower alkyl group; (ii) a nitrogen-containing aliphatic heteromonocyclic group optionally substituted by a hydroxyl group; and (iii) an amino group substituted by a group selected from a lower alkyl group, a cyclo-lower alkylcarbonyl group, a (mono- or di-lower alkyl)amino-lower alkyl group, a lower alkanoyl group, a heteroarylcarbonyl group, a lower alkylsulfonyl group and a lower alkyl-thiocarbamoyl group, or (c) a nitrogen-containing aliphatic heteromonocyclic group substituted by a group selected from (i) a lower alkyl group, (ii) an amino group optionally substituted by a group selected from a lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkyl group and a cyclo-lower alkylcarbonyl group and (iii) a nitrogen-containing aliphatic heteromonocyclic group substituted by a lower alkyl group, R2 is a phenyl group substituted by a group selected from a halogen atom and a lower alkoxy group, a lower alkyl group-substituted heteroaryl group or a lower alkoxy-substituted heteroaryl group and Q is methylene group.
Among the more preferred compounds [II] mentioned above, further preferred compound are those in which the group of the formula: R1—(O)q—Y-Z- is a 4-(mono- or di-lower alkylamino-lower alkyl)benzoyl group; a 4-(pyrrolidino-lower alkyl)benzoyl group; a 4-(di-lower alkylamino-lower alkoxy)benzoyl group; a 3-(di-lower alkylamino-lower alkoxy)-4-(di-lower alkylamino-lower alkoxy)benzoyl group; a 4-(piperidino-lower alkoxy)benzoyl group; a 4-[N-lower alkyl-N-(di-lower alkylamino-lower alkyl)-amino]benzoyl group; a 4-[N-lower alkanoyl-N-(di-lower alkylamino-lower alkyl)-amino]benzoyl group; a 4-[N-lower alkenoyl-N-(di-lower alkylamino-lower alkyl)-amino]benzoyl group; a 4-[N-(cyclo-lower alkylcarbonyl)-N-(di-lower alkylamino-lower alkyl)amino]benzoyl group; a 4-[N-(lower alkoxy-lower alkoxycarbonyl)-N-(di-lower alkylamino-lower alkyl)amino]benzoyl group; a 4-[N-lower alkanoyl-N-(pyrrolidino-lower alkyl)amino]benzoyl group; a [1-(lower alkyl)piperidin-4-yl]-carbonyl group; a 4-[N-lower alkyl-N-(di-lower alkylamino-lower alkyl)amino]-piperidinocarbonyl group; a 4-[N-(cyclo-lower alkylcarbonyl)-N-(di-lower alkylamino-lower alkyl)amino]-piperidinocarbonyl group; a 4-[4-(di-lower alkyl)piperidino]-piperidinocarbonyl group; a [1-(lower alkyl)piperidin-4-yl]lower alkanoyl group; a [1-(lower alkyl)piperidin-4-yl]lower alkenoyl group; a 4-(di-lower alkylamino-lower alkyl)cyclohexylcarbonyl group; a 4-(mono- or di-lower alkylamino)cyclohexylcarbonyl group; a 4-[N-lower alkanoyl-N-(di-lower alkylamino-lower alkyl)amino]-cyclohexylcarbonyl group; a 4-[N-lower alkenoyl-N-(di-lower alkylamino-lower alkyl) amino]cyclohexylcarbonyl group; a 4-[N-heteroarylcarbonyl-N-(di-lower alkylamino-lower alkyl)amino]cyclohexylcarbonyl group; a 4-[N-lower alkylthiocarbamoyl-N-(di-lower alkylamino-lower alkyl)amino]cyclohexylcarbonyl group; a 4-[N-(di-lower alkylamino-lower alkyl)-N-(lower alkylsulfonyl)amino]cyclohexylcarbonyl group; a 4-[[N-lower alkyl-N-(hydroxy-lower alkyl)amino]lower alkyl]cyclohexylcarbonyl group; a 4-[[N-lower alkyl-N-lower alkoxy-lower alkyl)amino]lower alkyl]cyclohexylcarbonyl group; a 4-[[N-lower alkanoyl-N-(di-lower alkylamino-lower alkyl)amino]-lower alkyl]cyclohexylcarbonyl group; a 4-[[N-(cyclo-lower alkylcarbonyl)-N-(di-lower alkylamino-lower alkyl)amino]lower alkyl]cyclohexylcarbonyl group; a 4-(pyrrolidino)-cyclohexylcarbonyl group; a 4-(hydroxypyrrolidino)cyclohexylcarbonyl group; or a 4-(piperidino)cyclohexylcarbonyl group, and R2 is a phenyl group substituted by one or two groups selected from an ethoxy group and a fluorine atom, an ethoxypyridyl group, a propylpyridyl group or a propylthiazolyl group.
Among the further preferred compounds [I] of the present invention mentioned above, particularly preferred examples are those in which R2 is 3-ethoxyphenyl group, 6-propylpyridin-2-yl group, 6-ethoxypyridin-2-yl group, 2-propyl-1,3-thiazol-4-yl group or 3-ethoxy-2-fluorophenyl group.
The concrete examples of the particularly preferred compound include
When the compound [I] of the present invention has an asymmetric carbon atom(s) at the substituent in R1 and/or R2, it may exist in the form of a stereoisomer thereof (diastereoisomers, optical isomers) owing to said asymmetric carbon atom(s) thereof, and the present invention also includes these stereoisomers and a mixture thereof.
A compound [I] of the present invention or a pharmaceutically acceptable salt thereof shows a significant antagonizing activity against apamin, which is known as a selective SK channel blocker, in a competitive binding assay. For example, as a result of the assay conducted in the same manner as described in WO02/079189 (Experiment 1), a compound of the present invention, 1-(3-ethoxybenzyl)-4-[4-[[N-(cyclopropyl-carbonyl)-N-[2-(dimethylamino)ethyl]amino]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine, showed an excellent apamin-binding inhibitory activity (IC50: 0.05 μM. Therefore, the compounds [I] or a pharmaceutically acceptable salt thereof is useful as a SK channel blocker which is applicable to treatment and/or prophylaxis of SK-channel-related diseases such as gastrointestinal motility disorders (e.g., constipation, irritable bowel syndrome, gastoroesophageal reflux disease, post-operative ileus), central nervous system disorders (e.g., memory and learning disorders including Arzheimer's disease, depression), emotional disorders, myotonic muscular dystrophy or sleep apnea.
Moreover, the compound of the present invention shows a low toxicity and are safe as medicaments.
The compound [I] of the present invention can be clinically used either in the free form or in the form of a pharmaceutically acceptable salt thereof. The pharmaceutically acceptable salt of the compound [I] includes a salt with an inorganic acid such as hydrochloride, sulfate, phosphate or hydrobromide, or a salt with an organic acid such as oxalate, citrate, methanesulfonate, benzenesulfonate, tosylate or maleate. Besides, when the compounds [I] of the present invention has a carboxyl group(s) and the like in its molecule, examples of the pharmaceutically acceptable salt include, salts with a base such as alkaline metal (e.g., sodium salt, potassium salt), or alkaline earth metal (e.g., calcium salt).
The compound [I], a salt thereof, or its intermediate or a salt thereof includes either intramolecular salt or an additive thereof, and solvates or hydrates thereof.
The present compound [I] or a pharmaceutically acceptable salt thereof can be administered either orally or parenterally, and can be formulated into a conventional pharmaceutical preparation such as tablets, granules, fine granules, capsules, powders, injections or inhalants.
The dose of the compounds [I] of the present invention or a pharmaceutically acceptable salt thereof may vary in accordance with the administration routes, and the ages, weights and conditions of the patients. For example, when administered in an injection preparation, it is usually in the range of about 0.0001 to 1 mg/kg/day, preferably in the range of about 0.001 to 0.1 mg/kg/day. When administered in an oral preparation, it is usually in the range of about 0.001 to 100 mg/kg/day, preferably in the range of 0.01 to 10 mg/kg/day.
The pyrazolopyrimidine compounds [I] of the present invention may be prepared by the following manners.
Process A:
Among the compounds [I] of the present invention, the compound of the following formula [I-A]:
wherein Za is a group of the formula: —CO—, —SO2— or ═C═N—CN and other symbols are the same as defined above, can be prepared by reacting a compound of the formula [II]:
wherein the symbols are the same as defined above or a salt thereof with a compound of the formula [III]:
R1—(O)q—Y-Za-OR3 [III]
wherein R3 is a hydrogen atom, a lower alkyl group or benzyl group and other symbols are the same as defined above or a salt thereof.
When R3 is a hydrogen atom, the above-mentioned reaction can be carried out in a solvent in the presence of a condensing agent, and in the presence or absence of an activating agent and a base. Examples of the solvent include any solvent which does not disturb the reaction, such as methylene chloride, chloroform, N,N-dimethylformaide, N,N-dimethylacetamide, tetrahydrofuran, dioxane, toluene, benzene, 1,2-dichloroethane, 1-methyl-2-pyrrolidinone, 1,2-dimethoxyethane and the like.
The condensing agent includes, for example, dicyclohexylcarbodiimide (DCC), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide.hydrochloride (WSC.HCl), diphenyl-phosphoryl azide (DPPA), carbonyldiimidazole (CDI), diethylcyano-phosphonate (DEPC), diisopropylcarbodiimide (DIPCI), benzotriazol-1-yloxy-trispyrrolidinophosphonium hexafluorophosphate (PyBOP), carbonylditriazole, N-cyclohexylcarbodiimide-N′-propyloxymethylpolystylene (PS-carbodiimide), N-ethoxy-carbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ), 2-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU), 2-(1H-benzotriazol-1-yl-1,1,3,3-tetramethyluronium (HBTU), bromotrispyrrolidinophosphonium hexafluorophosphate (PyBroP), 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU), chloro-1,1,3,3-tetramethyluronium chloroantimonate (ACTU) and the like.
Examples of the activating agent include 1-hydroxybenzotriazole (HOBt), 1-hydroxysuccinimide (HOSu), dimethylaminopyridine (DMAP), 1-hydroxy-7-azabenzotriazole (HOAt), hydroxyphthalimide (HOPht), pentafluorophenol (Pfp-OH), 1-hydroxybenzotriazole-6-sulfonamidomethylpolystylene (PS-HOBt) and the like.
The base includes, for example, pyridine, triethylamine, diisopropylethylamine, 4-methylmorpholine, 1,8-diazabicyclo[5,4,0]-7-undecene (BU) and the like.
In the above-mentioned process, the compound [II] can be used in an amount of 0.3 to 10 moles, preferably 0.5 to 2 moles per mole of the compound [I]. The condensing agent can be used in an amount of 1 to 10 moles, preferably 1.5 to 4 moles per one mole of the compound [II] or [III]. The base can be used in an amount of 1 to 10 moles, preferably 2 to 4 moles per one mole of the compound [II] or [x]. The activating agent can be used in an amount of 1 to 10 moles, preferably 1.5 to 4 moles per one mole of the compound [II] or [III]. The reaction can be carried out at −20 to 80° C., preferably 0 to 30° C.
Concomitantly, when R3 in the compound [III] is hydrogen atom, the reaction process A to prepare the compound [I-A] can be carried out by converting the compound to a reactive derivative at the carboxyl group and the like (e.g., an acid halide, a mixed acid anhydride) and reacting the reactive derivative with the compound [II] in the presence of the base in the solvent or without solvent.
When R3 in the compound [III] is a lower alkyl group or a benzyl group, the reaction process A can be also carried out by converting the compound to a corresponding carboxylic acid (sulfonic acid or imino acid) compound by a conventional method such as hydrolysis, acidolysis using hydrochloric acid, formic acid, trifluoroacetic acid and the like or hydrogenation and reacting the carboxylic acid compound with the compound [II] by the above-mentioned method.
Furthermore, when R3 in the compound [III] is a lower alkyl group or a benzyl group, the reaction process A can be also carried out by directly reacting the ester compound [II] with the compound [III] in the presence of a base in a solvent or without solvent. The solvent includes any solvent which does not disturb the reaction, such as methylene chloride, chloroform, N,N-dimethylformamide, N,N-dimethylacetamide, tetrahydrofuran, dioxane, toluene, benzene, 1,2-dichloroethane, 1-methyl-2-pyrrolidinone, methanol, ethanol, isopropanol and the like. The base includes, for example, triethylamine, diisopropylethylamine, 4-methylmorpholine, 1,8-diazabicyclo-[5,4,0]undecene (DBU), dimethylaminopyridine (DMAP) and the like.
In the above-mentioned process, the compound [III] can be used in an amount of 0.3 to 10 moles, preferably 0.5 to 2 moles per mole of the compound [II]. The base can be used in an amount of 1 to 10 moles, preferably 1 to 4 moles per one mole of the compound [II] or [III]. The reaction can be carried out at 25 to 150° C., preferably 60 to 100° C.
Process B:
Among the compounds [I] of the present invention, the compound in which Z is —CH2— of the following formula [I-B]:
wherein the symbols are the same as defined above, can be prepared by reacting a compound [II] or a salt thereof with an aldehyde compound (a lower alkanal or an aryl-lower alkanal) of the formula [IV]:
R1—(O)q—Y—CHO [IV]
wherein the symbols are the same as defined above in the presence of a reducing agent in a solvent.
The solvent includes any solvent which does not disturb the reaction, such as methylene chloride, chloroform, 1,2-dichloroethane, N,N-dimethylformamide, N,N-dimethylacetamide, 1-methyl-2-pyrrolidinone, tetrahydrofuran, 1,2-dimethoxyethane, dioxane, toluene, benzene, methanol, ethanol, isopropanol, acetic acid and the like. The reducing agent includes, for example, macroporous triethylammoniummethyl-polystyrene cyanoborohydride (MP-Cyanoborohydride), sodium borohydride, sodium triacetoxyborohydride, sodium cyanoborohydride and the like. The reducing agent can be used in an amount of 1 to 10 moles, preferably 1 to 4 moles per one mole of the compound [II]. The reaction can be carried out at −20 to 100° C., preferably 0 to 40° C.
Process C:
Among the compounds [I] of the present invention, the compound of the formula [I-C]:
wherein R11 is an amino group optionally substituted by a group selected from a lower alkyl group, a (mono- or di-lower alkyl)amino-lower alkyl group and a nitrogen-containing aliphatic heteromonocyclic group-substituted lower alkyl group, Za is a group of the formula: —CO—, —SO2— or ═C═N—CN and other symbols are the same as defined above, can be prepared by reacting a carboxylic acid compound of the formula [V]:
wherein the symbols are the same as defined above or a salt thereof with an amine compound of the formula [VI]:
R11—H [V]
wherein R11 is the same as defined above or a salt thereof. The reaction can be carried out in a solvent in the presence of a condensing agent and in the presence or absence of an activating agent and a base. Examples of the solvent, condensing agent, activating agent and base include those as exemplified in the Process A.
The compound [V] can be used in an amount of 0.5 to 3 moles, preferably 1 to 2 moles per one mole of the compound [VI]. The condensing agent can be used in an amount of 1 to 10 moles, preferably 1.5 to 4 moles per one mole of the compound [V] or [VI]. The base can be used in an amount of 1 to 10 moles, preferably 2 to 4 moles per one mole of the compound [V] or [VI]. The activating agent can be used in an amount of 1 to 10 moles, preferably 1.5 to 4 moles per one mole of the compound [V] or [VI]. The reaction can be carried out at −20 to 80° C., preferably 0 to 30° C.
Process D:
Among the compounds [I] of the present invention, the compound of the formula [I-D]:
wherein R12 is an optionally substituted nitrogen-containing aliphatic heteromonocyclic group or an optionally substituted amino group and other symbols are the same as defined above can be prepared by reacting a compound of the formula [VII]:
wherein W2 is a reactive residue and other symbols are the same as defined above with a compound of the formula [VIII]:
wherein the symbol is the same as defined above or a salt thereof, or reacting a compound of the formula [II]:
wherein the symbols are the same as defined above or a salt thereof with a compound of the formula [IX]:
wherein the symbol is the same as defined above.
The reaction for preparing the compound [I-D] from the compound [VII] and [VIII] or the compound [II] and [IX] can be carried out in a solvent in the presence of a base. The solvent includes any solvent which does not disturb the reaction, such as chloroform, methylene chloride, 1,2-dichloroethane, tetrahydrofuran, dioxane, ethyl acetate, N,N-dimethylformamide, N,N-dimethylacetamide, 1-methyl-2-pyrrolidinone, dimethylsulfoxide, toluene and the like. The base includes, for example, triethylamine, diisopropylethylamine, N-methylmorpholine, pyridine and the like. The compound [VIII] or [II] can be used in an amount of 0.8 to 3 moles, preferably 1 to 1.5 moles per one mole of the compound [VII] or [IX]. The base can be used in an amount of 1 to 4 moles, preferably 2 to 3 moles per one mole of the compound [II] or [IX]. The reaction can be carried out at 25 to 150° C., preferably 60 to 120° C.
Meanwhile, the compound [IX] can be prepared by reacting the compound [II] with a compound of the formula (phosgen, a phosgen precursor, a phosgen equivalent and the like):
wherein W1 and W2 are a reactive residue.
Besides, the compound [IX] can be prepared by reacting the compound [VIII with the compound [X]. In the compound [X], the reactive residue represented as W1 or W2 may be a halogen atom, phenoxy group, p-nitrophenoxy group, a lower alkoxy group or a nitrogen-containing aromatic heteromonocyclic group (e.g., a 5- or 6-membered heteroaryl group containing at least one nitrogen atom such as pyridyl group).
The reaction for preparing the compound [VII] or [IX] can be carried out in a solvent in the presence of a base. The solvent and the base include those used in the reaction for preparing the compound [I-D] from the compound [VII] and [VII] or the compound [II] and [IX]. The phosgen precursor is, for example, triphosgen, diphosgen (phosgen dimer) and the like. The phosgen equivalent includes, for example, p-nitrophenyl chloroformate, diethyl carbonate, 1,1′-carbonyldiimidazole, diphenyl chlorocarbonate, diethyl carbonate, N,N′-disuccinimidyl carbonate, dipyridin-2-yl carbonate and the like.
The compound [α]can be used in an amount of 0.2 to 4 moles, preferably 0.4 to 2 moles per one mole of the compound [II] or [VIII]. The base can be used in an amount of 3 to 6 moles, preferably 3 to 4 moles per one mole of the compound [II] or [VIII]. The reaction can be carried out at −4 to 60° C., preferably 0 to 30° C.
The aforementioned reactions for preparing the compound [I-D] from the compound [VIII] via the compound [VII] or [IX] can be also carried out without isolating the intermediate compound [VII] or [IX].
The objective compound [I] of the present invention can be also prepared by intramolecularly converting the substituent(s) in R1 of the compounds [I] as obtained above to the other desired substituent(s) within the scope the present invention. The intramolecular conversion processes can be selected according to the kinds of the objective substituents, and may be carried out, for example, by the following methods (a) to (e).
Method (a):
An objective compound [I] of the present invention having a substituent(s) containing a substituted lower alkoxy group in R1 can be prepared by reacting a compound [I] having a substituent(s) containing a hydroxyl group in R1 with a lower alkyl halide having a corresponding substituent(s) in the presence of a base (e.g., sodium hydride, potassium carbonate), or by reacting a compound [I] having a substituent(s) containing a hydroxyl group in R1 with a lower alkanol having a corresponding substituent(s) in an appropriate solvent in the presence of triphenylphosphine and isopropyl azodicarboxylate.
Method (b):
An objective compound [I] of the present invention having a substituent(s) containing a lower alkylamino group in R1 can be prepared by reacting a corresponding compound [I] having a substituent(s) containing a primary or secondary amino group with a corresponding lower alkyl halide in an appropriate solvent in the presence of a base.
Method (c):
An objective compound [I] of the present invention having a substituent(s) containing an acylamino group such as a lower alkanoylamino group in R1 can be prepared by reacting a corresponding compound [I] having a substituent(s) containing a primary or secondary amino group in R1 with a corresponding carboxylic acid or thiocarboxylic acid compound in the same manner as described in the above Process A.
Method (d):
An objective compound [I] of the present invention having a substituent(s) containing a group of the formula:
wherein R13 is a hydrogen atom or a lower alkyl group, R14 and R15 are a lower alkyl group which may be combined each other to form a ring group and n is an integer of 1 or 2 in R1, can be prepared by reacting a corresponding compound [I] having a substituent(s) containing a primary or secondary amino group in R1 with a compound of the formula [X′]:
wherein X1 and X2 is a halogen atom and the other symbols are the same as defined above in an appropriate solvent in the presence of a base and then reacting the resultant product with an amine compound of the formula [XI]:
(R14)(R15)NH [XI]
wherein the symbols are the same as defined above. Meanwhile, the reaction can be also carried out in a single reaction vessel without isolating an intermediate.
Method (e):
An objective compound [I] of the present invention having a substituent(s) containing a group of the formula:
wherein m is an integer of 5 or 6 in R1 can be prepared by reacting a corresponding compound [I] having a substituent(s) containing an amino group(s) with a compound of the formula [XII]:
X3—(CH2)m-X4 [XII]
wherein X3 and X4 are a halogen atom and the other symbols are the same as defined above in an appropriate solvent in the presence of a base.
In conducting the above mentioned Processes A to C and Methods (a) to (e), when the starting materials or intermediate compounds have a functional group(s), if necessary, the protection of the functional groups and the following deprotection thereof may be carried out in accordance with a conventional method.
An intermediate compound [II] for preparation of the objective compounds [I] of the present invention can be obtained, for example, in accordance with the following method as described in WO02/79189, reacting a compound [XIII] with a compound [XIV] to prepare a compound [II-A], reacting the product with a compound [XV] to prepare a compound [II-B] and removing an amino-protecting group (G) from the resultant product in a conventional manner.
In the above scheme, G is an amino-protecting group and the other symbols are the same as defined above.
The reaction process to prepare the compound [II-A] can be carried out in a solvent or without solvent in the presence or absence of an activating agent and an additive. The solvent includes any solvent which does not disturb the reaction, for example, xylene, chloroform and the like. Examples of the activating agent include hexamethyldisilazane, N,O-bistrimethylsilylacetamide, chlorotrimethylsilane. The additives include ammonium sulfate, chlorotrimethylsilane, triethylamine hydrochloride, pyridine hydrochloride, triethylamine and the like.
The compound [II-B] can be prepared by reacting a compound [II-A] with a lower alkanol or a compound having a leaving group of the formula [XV]:
R2-Q-X5 [XV]
wherein X5 is hydroxyl group or a leaving group and the other symbols are the same as defined above in an appropriate solvent in the presence of an dehydrating agent or a base. The solvent includes, for example, methylene chloride, chloroform, 1,2-dichloroethane, N,N-dimethylformamide, N,N-dimethylacetamide, 1-methyl-pyrrolidinone, tetrahydrofuran, 1,2-dimethoxyethane, 1,4-dioxane, toluene, benzene and the like. The dehydrating agent for the compound in which X5 is a hydroxyl group includes, for example, a combination of a diester of azodicarboxylic acid and a tri-substituted phosphine or phosphorane and the like. The base for the compound in which X5 is a leaving group such as a halogen atom, a lower alkylsulfonyloxy group or an arylsulfonyloxy group includes, for example, an alkaline metal hydroxide such as lithium hydroxide, an alkali metal hydride, an alkali metal carbonate, an alkali metal lower alkoxide, lithium diisopropylamide (LDA) and the like.
In the above compound [II-B], amino-protecting group G includes, for example, benzyl group, a lower alkoxycarbonyl group and the protecting group can be removed in a conventional manner.
The intermediate compound [V] of the present invention can be prepared by reacting a compound [II] with a compound of the formula [XVI]:
R16—COOH [XVI]
wherein R16 is a phenyl group substituted by a protected or esterified carboxyl group or its reactive derivative such as a corresponding acid halide (e.g., an acid chloride) and removing the protecting group or ester residue from the resultant product in accordance with a conventional manner.
The objective compound [I] and the starting materilas [III] or [IV] of the present invention can be also prepared by intramolecularly converting the substituent(s) in R1 and/or R2 of the compound as obtained above to the other desired substituent(s) within the scope the present invention. The method of such conversion can be selected according to the kind of the desired substituent and includes, for example, O-alkylation, reductive amination and the like.
If necessary, the compounds [I] of the present invention obtained in the aforementioned Processes A to D or Methods (a) to (e) can be converted to a pharmaceutically acceptable salt thereof by a conventional manner.
Throughout the present description and claims, the “lower alkyl group” or “lower alkoxy group” means a straight or branched chain alkyl or alkoxy group having 1 to 6 carbon atoms, preferably 1 to 4 carbon atoms. The “lower alkanoyl group” means a straight or branched chain alkanoyl group having 2 to 7 carbon atoms, preferably 2 to 5 carbon atoms. The “cyclo-lower alkyl group” means a cycloalkyl having 3 to 8 carbon atoms, preferably 3 to 6 carbon atoms. The “lower alkenyl group” means a straight or branched chain alkenyl group having 2 to 8 carbon atoms, preferably 2 to 4 carbon atoms. The “lower alkynyl group” means a straight or branched chain alkynyl group having 2 to 8 carbon atoms, preferably 2 to 4 carbon atoms. The “lower alkylene group” means a straight or branched chain alkylene group having 1 to 6 carbon atoms, preferably 1 to 5 carbon atoms. The “lower alkenylene group” means a straight or branched chain alkenylene group having 2 to 8 carbon atoms, preferably 2 to 4 carbon atoms. Moreover, “halogen atom” means fluorine, chlorine, bromine or iodine atom. The “lower alkenoyl group” means a straight or branched chain alkenoyl group having 2 to 8 carbon atoms, preferably 3 to 6 carbon atoms. The “heteroaryl group” means a 5- to 14-membered heteroaryl group containing at least one heteroatom selected from nitrogen atom, sulfur atom or oxygen atom, particularly a 5- to 10-membered, mono- or bi-cyclic heteroaryl group containing at least one nitrogen atom as a heteroatom.
The present invention is illustrated in more detail by the following Examples and Reference Examples but should not be construed to be limited thereto.
To a solution of ethyl 4-[N-(cyclopropylcarbonyl)-N-[2-(dimethylamino)ethyl]-amino]benzoate (106 mg; compound obtained in Reference Example 11) in ethanol (3 mL) is added 2N sodium hydroxide solution (21 μL) and the mixture is stirred at room temperature overnight. To the reaction mixture is added 2N HCl (42 μL) and the mixture is concentrated to give a crude carboxylic acid compound. To the compound is added successively methylene chloride (2 mL), 1-(3-ethoxybenzyl)-4-piperazin-1-yl-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (105 mg, compound described in WO02/79189), 1-hydroxybenzotriazole (51.7 mg), triethylamine (124.5 μL) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (73.4 mg) and the mixture is stirred at room temperature for 19 hours. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution (10 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by flash column chromatography on NH-silica gel (Chromatorex NH silica gel; Fuji Silicia Chemical Ltd., Solvent; ethyl acetate:chloroform=1:0→0:1) to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-(cyclopropyl-carbonyl)-N-[2-(dimethylamino)ethyl]amino]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (127 mg, yield; 84%) as an amorphous powder. MS (APCI) m/z: 597 [M+H]+
To trans-4-(1-pyrrolidinyl)cyclohexanecarboxylate hydrochloride (26 mg; compound obtained in Reference example 87(2)) is added successively methylene chloride (3.5 mL), 1-(3-ethoxybenzyl)-4-piperazin-1-yl-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (30 mg), 0.5M 1-hydroxybenzotriazole in N,N-dimethylformamide (219 μL), triethylamine (35.6 μL) and N-cyclohexylcarbodiimide-N′-propyloxymethylpolystylene (310.4 mg, PS-Carbodiimide, 0.94 mmol/g, Argonaut Technology) and the mixture is stirred at room temperature for 24 hours by using a parallel synthesizer (Quest 210; Argonaut Technology). To the reaction mixture is added macroporous triethylammoniummethylpolystylene carbonate (120 mg, MP-Carbonate 3.04 mmol/g; Argonaut Technology) and the mixture is stirred at room temperature for 3 hours. The liquid layer is separated from the reaction mixture and the residual resin is washed successively with chloroform and methanol. The liquid layer and the washings are combined and concentrated by centrifugal concentrator. The resultant crude product is purified by HPLC (XTerra PerpMS C18 column; Waters Inc., Solvent; 10 mM ammonium carbonate:methanol=1:1→5:95) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(1-pyrrolidinyl)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]-pyrimidine (27 mg, yield; 71%) as an amorphous powder.
MS (APCI) m/z: 518 [M+H]+
IR(Nujol) cm−1; 1634, 1596, 1555, 1459
To 4-[2-(dimethylamino)ethoxy]benzoic acid hydrochloride (66 mg, compound obtained in Reference example 78(2)) is added successively methylene chloride (2 mL), 1-(3-ethoxybenzyl)-4-piperazin-1-yl-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (100 mg), 1-hydroxybenzotriazole (49.3 mg), triethylamine (118.6 μL) and 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride (70 mg) and the mixture is stirred at room temperature for 17 hours. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution (10 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by flash column chromatography on NH-silica gel (Solvent; ethyl acetate:chloroform=1:0→0:1) to give 1-(3-ethoxybenzyl)-4-[4-[4-[2-(dimethylamino)ethoxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (103 mg, yield; 69%) as an amorphous powder. MS (APCI) m/z: 530 [M+H]+
(1) To a suspension of 1-(3-ethoxybenzyl)-4-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (2 g) and triethylamine (2.18 mL) in methylene chloride (20 mL) is added dropwise a solution of terephthalic acid mono-methyl ester chloride (970 mg) in methylene chloride (10 mL) under ice-cooling and the mixture is stirred at room temperature for one day. The reaction mixture is diluted with water and stirred for 30 minutes. The methylene chloride layer is separated and the aqueous layer is extracted with chloroform. The chloroform layer and the methylene chloride layer are combined, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated. The crude product is purified by flash column chromatography on silica gel (Biotage Flush 40M; Solvent; ethyl acetate:n-hexane=1:1) to give 1-(3-ethoxybenzyl)-4-[4-[4-(methoxycarbonyl)benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]-pyrimidine (2.4 g, yield; 98%) as an amorphous powder. MS (APCI) m/z: 501 [M+H]+
(2) To a suspension of the compound obtained in the above step (1) (2.4 g) in ethanol/tetrahydofuran (1:1, 25 mL) is added dropwise 10% aqueous sodium hydroxide solution (10 mL) and the mixture is stirred at room temperature for 19 hours. The reaction mixture is diluted with chloroform, neutralized with 10% aqueous citric acid solution and extracted with chloroform (×2). The extract is washed with saturated brine, dried over anhydrous sodium sulfate and concentrated. To the resultant crude carboxylic acid compound (50 mg) is added successively N,N-diethylethylenediamine (35.8 mg), 0.5 M 1-hydroxybenzotriazole in N,N-dimethylformamide (308 μL) and N-cyclohexylcarbodiimide-N′-propyloxymethylpolystylene (328 mg, PS-carbodiimide 0.94 mmol/g). The mixture is stirred at room temperature for 24 hour by using a parallel synthesizer (Quest 210). To the reaction mixture is added macroporous triethylammoniummethylpolystylenecarbonate (MP-Carbonate 3.04 mmol/g, 120 mg) and the mixture is stirred at room temperature for 1 hour. The liquid layer is separated and the resin is washed successively with chloroform and methanol. The washing and the reaction solution are combined and concentrated. The resultant crude product is purified by HPLC (XTerra PrepMS C18 column, Solvent; 10 mM ammonium carbonate:methanol=80:20→5:95) to give 1-(3-ethoxybenzyl)-4-[4-[4-[2-(diethylamino)ethylcarbamoyl]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]-pyrimidine (15.6 mg, yield; 19%) as an amorphous powder. MS (APCI) m/z: 585 [M+H]+
(3) The compound obtained in the above step (2) is dissolved in ethanol (1 mL) and thereto is added 2N hydrochloric acid (16 μL). The mixture is evaporated to remove solvent and the residue is dissolved in water and lyophilized to give 1-(3-ethoxybenzyl)-4-[4-[4-[2-(diethylamino)ethylcarbamoyl]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride as an amorphous powder. MS (APCI) m/z: 585 [M+H]+
(1) The compound obtained in Reference Example 1(3) is treated in the same manner as described in Example 1 to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-[3-(dimethylamino)propionyl]-N-[2-(dimethylamino)ethyl]amino]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine as an amorphous powder.
(2) A mixture of the compound obtained in the above step (1) (131 mg) and fumaric acid 24.2 mg is dissolved in water and lyophilized to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-[3-(dimethylamino) propionyl]-N-[2-(dimethylamino)ethyl]amino]benzoyl]-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine fumarate as powder.
MS (APCI) m/z: 628 [M+H]+
To 4-[N-[(2-methoxyethoxy)carbonyl]-N-[2-(dimethylamino)ethyl]amino]-benzaldehyde (90 mg, compound obtained in Reference Example 97) is added successively tetrahydrofuran (1.5 mL), 1-(3-ethoxybenzyl)-4-(1-piperazinyl)-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (100 mg), triethylamine (90 mL), acetic acid (73 μL) and macroporous triethylammoniummethylpolystylenecyanoborohydride (MP-Cyanoborohydride 2.04 mmol/g, Argonaut Technology). The mixture is shaken at room temperature for 18 hours by using a parallel synthesizer (MiniBlock; Mettler Toledo). The reaction mixture is separated and the residual resin is washed with tetrahydrofuran (×3). The washings and the reaction solution are combined and concentrated. The resultant crude product is purified by flash column chromatography on silica gel (Solvent; ethyl acetate:chloroform=1:0→0:1) to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-[(2-methoxyethoxy)carbonyl]-N-[2-(dimethylamino)ethyl]-amino]benzyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (159 mg, yield; 38%) as an amorphous powder. MS (APCI) m/z: 617 [M+H]+
The corresponding materials are treated in the same manner as described in Example 1, or Examples 1 and 4(2) to give the compounds as shown in the following Table 1.
*hydrochloride
Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
*hydrochloride, Me: methyl group, Et: ethyl group, Boc: tert-butoxycarbonyl group
*hydrochloride
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 3, or Examples 3 and 5(2) to give the compounds as shown in the following Table 3.
*fumarate
Me: methyl group, Et: ethyl group
*fumarate
Me: methyl group, Et: ethyl group, nPr: n-propyl group
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*fumarate
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*fumarate
Me: methyl group, Et: ethyl group, nPr: n-propyl group
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Examples 4 and 5(2) to give the compounds as shown in the following Table 4.
*hydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 6 to give the compounds as shown in the following Table 5.
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in either one of Examples 1 to 6 to give the compounds as shown in the following Table 6.
*hydrochloride
The corresponding materials are treated in the same manner as described in Example 1, or Examples 1 and 4(3) to give the compounds as shown in the following Table 7.
Me: methyl group, Et: ethyl group
Me: methyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride,
**dihydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride, **dihydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, nBu: n-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nBu: n-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group, Boc: tert-butoxycarbonyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, nBu: n-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, nBu: n-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, nBu: n-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, nBu: n-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, nBu: n-butyl group
*hydrochloride,
**dihydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group
The corresponding materials are treated in the same manner as described in Example 2 to give the compound as shown in the following Table 8.
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Examples 3, Examples 3 and 4(3) to give the compounds as shown in the following Table 9.
Me: methyl group, Et: ethyl group, nPr: n-propyl group
Me: methyl group, Et: ethyl group, nPr: n-propyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride, Me: methyl group, Et: ethyl group
**dihydrochloride, Me: methyl group
To a solution of tert-butyl 4-[N-acetyl-N-[2-(1-pyrrolidinyl)ethyl]amino]-benzoic acid (37 mg, compound obtained in Reference Example 107) in methylene chloride (0.2 mL) is added trifluoroacetic acid (0.2 mL), and the mixture is shaken at room temperature for one day. The reaction mixture is concentrated and the residue is subjected to azeotropic distillation successively with chloroform, 4N HCl-dioxane and chloroform to give 4-[N-acetyl-N-[2-(1-pyrrolidinyl)ethyl]amino]benzoate hydrochloride. The product and 1 (3-ethoxybenzyl)-4-piperazin-1-yl-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride are treated in the same manner (condensation reaction) as described in Example 3 and then the resultant product is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-acetyl-N-[2-(1-pyrrolidinyl)ethyl]amino]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride (15 mg, yield; 21%) as an amorphous powder. MS (APCI) m/z; 597 [M+H]+
The corresponding materials are treated in the same manner as describe in Example 366 to give the compounds as shown in the following Table 9.1.
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
(1) To 4-hydroxybenzoic acid (1.06 g) is added successively methylene chloride (30 mL), 1-(3-ethoxybenzyl)-4-piperazin-1-yl-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (3.02 g), 1-hydroxybenzotriazole (1.25 g), triethylamine (3.7 mL) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (1.78 g). The mixture is stirred at room temperature for 17 hours. The reaction mixture is diluted with chloroform and thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated and evaporated to remove solvent. The resultant residue is purified by flash column chromatography on silica gel (Solvent; chloroform:methanol=50:1) to give 1-(3-ethoxybenzyl)-4-[4-(4-hydroxybenzoyl)piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (2.70 g, yield; 80%) as an amorphous powder. MS (APCI) m/z: 459 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (250 mg), 3-dimethylamino-2,2-dimethyl-1-propanol (215 mg), and triphenylphosphine (429 mg) in tetrahydrofuran (4.0 mL) is added dropwise diisopropyl azodicarboxylate (330 mg) under ice-cooling and the mixture is stirred at room temperature overnight. The reaction mixture is diluted with methylene chloride (4 mL) and treated with cation-exchange resin (ISOLUTE SCX; IST Ltd., Solvent; methanol:methylene chloride=1:1→1N ammonia/methanol). The resultant crude product is purified by flash column chromatography on silica gel (Solvent; chloroform:methanol=10:1) and then the product is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[4-[3-(dimethylamino)-2,2-dimethylpropyloxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride (257 mg, yield; 78%) as an amorphous powder. MS (APCI) m/z: 572 [M+H]+
The corresponding materials are treated in the same manner as described in Example 381 to give the compounds as shown in the following Table 10.
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group, nPr: n-propyl group
(1) The corresponding compound is treated in the same manner as described in Example 381 to give 1-(3-ethoxybenzyl)-4-[[4-[[1-(tert-butoxycarbonyl)piperidin-1-yl]oxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (34 mg, yield; 61%) as an amorphous powder. MS (APCI) m/z: 642 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (34 mg) in methanol (0.5 mL) is added 4N HCl-dioxane (0.1 mL) and the mixture is stirred at room temperature overnight. The reaction mixture is evaporated to remove solvent. The resultant residue is dissolved in water and lyophilized to give 1-(3-ethoxybenzyl)-4-[4-[4-(4-piperidyloxy)benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine 2hydrochloride (27 mg, yield; 79%) as an amorphous powder. MS (APCI) m/z: 542 [M+H]+
The corresponding materials are treated in the same manner as described in Example 404 to give the compounds as shown in the following Table 11.
**dihydrochloride
Me: methyl group, Et: ethyl group
(1) Methyl 4-[[N-ethyl-N-(tert-butoxycarbonyl)]aminomethyl]benzoate (230 mg, compound obtained in Reference Example 81) is treated in the same manner as described in Example 1 to give 1-(3-ethoxybenzyl)-4-[4-[4-[[N-ethyl-N-(tert-butoxy-carbonyl)]aminomethyl]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (330 mg, 2 steps yield; 70%) as an amorphous powder. MS (APCI) m/z: 600 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (330 mg) in dioxane (2 mL) is added 4N HCl-dioxane (4 mL) and the mixture is stirred at room temperature for 6 hours. To the reaction mixture is added diisopropylether (6 mL,), and after stirring, the supernatant is removed (Such washing procedure is repeated again). The precipitate is dissolved in water (2 mL) and thereto is added a saturated sodium hydrogencarbonate solution (3 mL) and chloroform (3 mL). After standing, the organic layer is separated and concentrated. The resultant crude product is purified by flash column chromatography on NH-silica gel (Purif 8 Hi-flush/L, MORITEX Inc.; Solvent; n-hexane:ethyl acetate=65:35→0:100) to give 1-(3-ethoxybenzyl)-4-[4-[4-(ethylaminomethyl)benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (121 mg, yield; 44%) as an amorphous powder. MS (APCI) m/z: 500 [M+H]+
(3) To a suspension of the compound obtained in the above step (2) (110 mg) in water (500 μL) is added 2N HCl (220 μL) and the mixture is lyophilized to give 1-(3-ethoxybenzyl)-4-[4-[(ethylaminomethyl)benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]-pyrimidine dihydrochloride as an amorphous powder. MS (APCI) m/z: 500 [M+H]+
The corresponding materials are treated in the same manner as described in Example 407 to give the compounds as shown in the following Table 12.
**dihydrochloride, ***trihydrochloride
Me: methyl group, Et: ethyl group
To a solution of the compound obtained in Example 415 (50 mg) and triethylamine (50 mg) in methylene chloride is added acetyl chloride (10 mg) and the mixture is stirred at room temperature for 18 hours. The reaction mixture is diluted with a saturated sodium hydrogencarbonate solution and extracted with chloroform. The extract is evaporated to remove solvent and the resultant crude product is purified by HPLC (Solvent; 10 mM ammonium carbonate:methanol=80:20→5:95) and then the resultant product (1-(3-ethoxybenzyl)-4-[4-[[trans-4-[N-acetyl-N-[2-(dimethylamino)ethyl]amino]cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine) is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(N-acetyl-N-[2-(dimethylamino)ethyl]amino]cyclohexyl]-carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (20 mg, yield; 40%) as an amorphous powder. MS (APCI) m/z: 577 [M+H]+
The corresponding materials are treated in the same manner as described in Example 416 to give the compounds as shown in the following Table 13.
**dihydrochloride
Me: methyl group, Et: ethyl group
To a suspension of the compound obtained in Example 410 (40 mg) in tetrahydrofuran (1 mL) is added successively potassium carbonate (35 mg) and ethyl iodide (9 μL), and the mixture is stirred at room temperature overnight. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by HPLC (Solvent; 10 mM ammonium carbonate:methanol=80:20→5:95) and then the resultant product is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(N-ethyl-N-methylamino)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (13 mg, yield; 31%) as an amorphous powder. MS (APCI) m/z: 506 [M+H]+
The corresponding materials are treated in the same manner as described in Example 422 to give the compounds as shown in the following Table 14.
**dihydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, iPr: isopropyl group
(1) To a suspension of the compound obtained in Example 411 (50 mg) in dichloromethane (1 mL) is added successively triethylamine (44 μL) and chloroacetyl chloride (9 μL), and the mixture is stirred for 2 hours under ice-cooling. To the mixture is added 2M dimethylamine in tetrahydrofuran (0.3 mL) under ice-cooling and the mixture is stirred at room temperature overnight. The reaction mixture is diluted with chloroform (3 mL) and thereto is added a saturated sodium hydrogencarbonate solution (5 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by HPLC (Solvent; 10 mM ammonium carbonate:methanol=80:20→5:95) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[N,N-(dimethylglycyl)amino]cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (20.4 mg, yield; 40%) as an amorphous powder. MS (ESI) m/z: 549 [M+H]+
(2) The compound obtained in the above step (1) is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[N,N-(dimethyl-glycyl)amino]cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine 2 hydrochloride (21.5 mg, yield; 93%) as an amorphous powder. MS (APCI) m/z: 549 [M+H]+
The corresponding materials are treated in the same manner as described in Example 425 to give the compounds as shown in the following Table 15.
**dihydrochloride
Me: methyl group, Et: ethyl group
Me: methyl group
Et: ethyl group
(1) To a suspension of the compound obtained in Example 411 (40 mg) in tetrahydrofuran (1 mL) is added successively dimetylacetamide (0.2 mL), 1,5-diiodopentane (16.6 μL), and sodium carbonate (28 mg). The mixture is stirred at 70° C. for 23 hours. After cooling, the reaction mixture is diluted with chloroform (3 mL) and thereto is added a saturated sodium hydrogencarbonate solution (5 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by HPLC (Solvent; 10 mM ammonium carbonate:methanol=80:20→5:95) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(1-piperidyl)cyclohexyl]-carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (12.9 mg, yield: 34%). MS (ESI) m/z: 532 [M+H]+
(2) The compound obtained in the above step (1) is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(1-piperidyl)-cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (15.4 mg, yield; quantitatively) amorphous powder. MS (APCI) m/z: 532 [M+H]+
The corresponding materials are treated in the same manner as described in Example 434 to give the compound as shown in the following Table 16.
*fumarate
Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 407 to give the compounds as shown in the following Table 17.
**dihydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
**dihydrochloride,
Et: ethyl group, nPr: n-propyl group, iPr: isopropyl group
The corresponding materials are treated in the same manner as described in Example 3 to give compounds as shown in the following Table 18.
*hydrochloride
Me: methyl group,Et: ethyl group, nPr: n-propyl group, iPr: isopropyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, nPr: n-propyl group, iPr: isopropyl group
Me: methyl group, Et: ethyl group, nPr: n-propyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Et: ethyl group, n-Pr: n-propyl group
*: hydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 381 to give the compounds as shown in the following Table 20.
*hydrochloride
Me: methyl group, Et: ethyl group
*: hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 366 to give the compounds as shown in the following Table 21.
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, i-Bu: isobutyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
(1) Trans-4-[N-methyl-N-(tert-butoxycarbonyl)aminomethyl]cyclohexanecarboxylic acid (compound obtained in Reference Example 239) is treated in the same manner as described in Example 3 to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[N-methyl-N-(tert-butoxycarbonyl)aminomethyl]cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine as an amorphous powder. MS (APCI) m/z; 592 [+H]+
(2) The compound obtained in the above step (1) is treated in the same manner as described in Example 404(2) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(methyl-aminomethyl)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride as an amorphous powder. MS (APCI) m/z; 492 [M+H]+
(1) 4-[[2-(dimethylamino)ethyl]amino]-3-methylbenzoic acid hydrochloride (compound obtained in Reference Example 251) is treated in the same manner as described in Example 3 to give 1-(3-ethoxybenzyl)-4-[4-[3-methyl-4-[2-(dimethylamino)ethylamino]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine as an amorphous powder. MS (APCI) m/z; 543 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (50 mg) in 1,2-dichloroethane (1 mL) is added successively acetaldehyde (10 μL), acetic acid (10 μL) and sodium triacetoxyborohydride (29 mg) under ice-cooling. The mixture is stirred at room temperature overnight. To the reaction mixture is further added successively acetaldehyde (10 μL), acetic acid (10 μL), and sodium triacetoxyborohydride (29 mg). The mixture is stirred at room temperature overnight. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform. The extract is concentrated and the residue is purified by HPLC (Solvent; 10 mM ammonium carbonate:methanol=80:20→5:95). The resultant product is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[3-methyl-4-[N-ethyl-N-[2-(dimethylamino)ethyl]amino]benzoyl]-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine 2 hydrochloride (22 mg, yield; 37%) as an amorphous powder. MS (APCI) m/z; 571 [M+H]+
The corresponding materials are treated in the same manner as described in Example 618 to give the compounds as shown in the following Table 22.
*hydrochloride, Et: ethyl group, i-Pr: isopropyl group, i-Bu: isobutyl group
**dihydrochioride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, i-Bu: isobutyl group
(1) To a solution of 1-(3-ethoxybenzyl)-4-(1-piperadinyl)-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (60 mg) in methylene chloride (1.2 mL) is added N,N-diisopropylethylamine (85 μL) and 4-nitrophenyl chloroformate (49 mg) at 0° C., and the mixture is stirred at room temperature for 2.5 hours. The reaction mixture is diluted with chloroform (1 mL) and thereto is added a saturated sodium hydrogencarbonate solution (1.5 mL). After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined, dried over sodium sulfate, and filtered. The filtrate is concentrated and the residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=9:1→0:10) to give 1-(3-ethoxybenzyl)-4-[4-[(4-nitrophenoxy)carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (72 mg, yield; 100%) as an amorphous powder. MS (APCI) m/z; 504 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (25 mg) in N,N-dimethylformamide (1.5 mL) is added N,N-diisopropylethylamine (51 μL) and 4-(1-pyrrolidinyl)piperidine (65 mg) at room temperature and the mixture is stirred at 70° C. for 4 hours. After cooling, the reaction mixture is diluted with chloroform (1 mL) and thereto is added a saturated sodium hydrogencarbonate solution (1.5 mL). After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined, dried over sodium sulfate, and filtered. The filtrate is concentrated and the residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=10:0→85:15) to give 1-(3-ethoxy-benzyl)-4-[4-[[4-(1-pyrrolidinyl)piperidin-1-yl]carbonyl]piperazin-1-yl]-1H-pyrazolo-[3,4-d]pyrimidine (19 mg, yield; 73%) as an amorphous powder. MS (APCI) m/z; 519 [M+H]+
(3) The compound obtained in the above step (2) (19 mg) is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[[4-(1-pyrrolidin-yl)piperidin-1-yl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine 2 hydrochloride as an amorphous powder. MS (APCI) m/z; 519 [M+H]+
The corresponding materials are treated in the same manner as described in Example 630 to give the compounds as shown in the following Table 23.
**dihydrochloride
Et: ethyl group
(1) Methyl 4-[[1-(tert-butoxycarbonylaminomethyl)-1-cyclopropyl]methoxy]-benzoate (compound obtained in Reference Example 235, 245 mg) is treated in the same manner as described in Example 1 to give 1-(3-ethoxybenzyl)-4-[4-[4-[[1-(tert-butoxycarbonylaminomethyl)-1-cyclopropyl]methoxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (380 mg, yield; 98%) as colorless liquid. MS (APCI) m/z; 642.5 [M+H]+
(2) The compound obtained in the above step (1) (100 mg) is treated in the same manner as described in Example 404(2) to give 1-(3-ethoxybenzyl)-4-[4-[4-[[1-(aminomethyl)-1-cyclopropyl]methoxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]-pyrimidine dihydrochloride (crude product) and the product is treated in the same manner as described in Example 618(2) 1-(3-ethoxybenzyl)-4-[4-[4-[[1-(dimethylaminomethyl)-1-cyclopropyl]methoxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]-pyrimidine hydrochloride (33 mg, yield; 34%) as an amorphous powder. MS (APCI) m/z; 570 [M+H]+
The corresponding materials are treated in the same manner as described in Example 633 to give the compounds as shown in the following Table 24.
*hydrochloride
Me: methyl group, Et: ethyl group
1-(3-Ethoxybenzyl)-4-[4-[3-methyl-4-[2-(dimethylamino)ethylamino]benzoyl]-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (compound obtained in Example 618(1)) is treated in the same manner as described in Example 416 to give 1-(3-Ethoxybenzyl)-4-[4-[3-methyl-4-[N-acetyl-N-[2-(dimethylamino)ethyl]amino]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (26 mg, yield; 42%) as an amorphous powder. MS (APCI) m/z; 585 [M+H]+
The corresponding materials are treated in the same manner as described in Example 637 to give the compounds as shown in the following Table 25.
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group
(1) Ethyl trans-4-(methoxymethoxy)cyclohexanecarboxylate (compound obtained in Reference Example 237, 2.0 g) is treated in the same manner as described in Example 1 to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(methoxymethoxy)cyclohexyl]-carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (2.88 g, yield; 78%) as an amorphous powder. MS (APCI) m/z; 509 [M+H]+
(2) To a suspension of the compound obtained in the above step (1) (2.8 g) in ethanol (20 mL) is added conc. hydrochloric acid (2.0 mL) and water (0.2 mL), and the mixture is stirred at 80° C. for 1 hour. The reaction mixture is diluted with ether and the resultant crystals are collected by filtration to give 1-(3-ethoxybenzyl)-4-[4-[(trans-4-hydroxycyclohexyl)carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride (2.81 g, yield; 99%) as colorless crystals. MS (APCI) m/z; 465 [M+H]+
(3) To a suspension of the compound obtained in the above step (2) (50 mg) in toluene (0.5 mL) is added 2-(dimethylamino)ethyl chloride hydrochloride (80 mg) and sodium hydride (60% dispersion in mineral oil, 50 mg), and the mixture is stirred at 100° C. for 2 days. To the reaction mixture is added water and the mixture is extracted with chloroform. The extract is concentrated in vacuo and the residue is purified by flash column chromatography on silica gel (Solvent; ethyl acetate) and then the resultant product is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[2-(dimethylamino)ethoxy]cyclohexyl]carbonyl]-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride (14 mg, yield; 24%) as an amorphous powder. MS (APCI) m/z; 536 [M+H]+
Methyl 4-[(2S)-2-(tert-butoxycarbonylamino)-4-methylpentyloxy]benzoate (compound obtained in Reference Example 285, 256 mg) is treated in the same manner as described in Example 1 to give 1-(3-ethoxybenzyl)-4-[4-[4-[(2S)-2-(tert-butoxy-carbonylamino)-4-methylpentyloxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]-pyrimidine (400 mg, yield; 99%) as a colorless oil. MS (APCI) m/z; 659 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (50 mg) in tetrahydrofuran (3 mL) is added sodium hydride (60% dispersion in mineral oil; 10 mg) under ice-cooling and the mixture is stirred for 0.5 hour. To the mixture is added dropwise methyl iodide (55 mg) and the mixture is stirred at room temperature for 3 hours. To the reaction mixture is added water and the mixture is extracted with ethyl acetate. The extract is concentrated and the residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=9:1→1:9) to give 1-(3-ethoxybenzyl)-4-[4-[4-[(2S)-2-[N-methyl-N-(tert-butoxycarbonyl)amino]-4-methylpentyloxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (35 mg, yield; 67%) as a colorless oil. MS (APCI) m/z; 672 [M+H]+
(3) The compound obtained in the above step (2) is treated in the same manner as described in Example 404(2) to give 1-(3-ethoxybenzyl)-4-[4-[4-[(2S)-2-(methyl-amino)-4-methylpentyloxy]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (15 mg, yield; 46%) as an amorphous powder. MS (APCI) m/z; 572 [M+H]+
The corresponding materials are treated in the same manner as described in Example 652 to give the compound as shown in the following Table 26.
**dihydrochloride
Me: methyl group, Et: ethyl group
(1) To a solution of oxalyl chloride (4.48 mL) in methylene chloride (50 mL) is added dropwise dimethylsulfoxide (4.55 g) in methylene chloride (5 mL) at −60° C. over a period of 15 minutes. To the mixture is added dropwise a solution of methyl trans-4-(hydroxymethyl)cyclohexanecarboxylate (compound obtained in Reference Example 238; 5.9 g) in methylene chloride (30 mL) over a period of 30 minutes. The mixture is stirred at the same temperature for 1 hour and thereto is added dropwise triethylamine (16.7 mL) at −60° C. The mixture is stirred at the same temperature for 30 minutes and at 0° C. for 1 hour. The reaction mixture is diluted with chloroform, and washed successively with water, 5% citric acid solution, water, and saturated brine. The organic layer is dried over anhydrous sodium sulfate and concentrated in vacuo to give methyl trans-4-formylcyclohexanecarboxylate (5.32 g, yield; 91%) as an oil.
(2) To a solution of the compound obtained in the above step (1) (500 mg) and 3-dimethylaminopropylamine (600 mg) in methylene chloride (10 mL) is added successively sodium triacetoxyborohydride (983 mg) and acetic acid (353 mg), and the mixture is stirred at room temperature for 5 days. The reaction mixture is neutralized carefully with a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform (×2). The extract is concentrated in vacuo and the residue is purified by flash column chromatography on silica gel (Solvent; chloroform methanol: 28% aqueous ammonia solution=9:1:0.1) to give methyl trans-4-[3-(dimethylamino)propylaminomethyl]cyclohexanecarboxylate (293 mg, yield; 32%) as oil. MS (APCI) m/z; 257 [M+H]+
(3) To a solution of the compound obtained in the above step (2) (92 mg) in chloroform (0.5 mL) is added a solution of 2-furoyl chloride (68 μL) in chloroform (0.5 mL) and pyridine (67 μL) under ice-cooling, and the mixture is stirred at room temperature for one day. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform (×3). The organic layers are combined and concentrated in vacuo. The resultant crude product is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=9:1→1:1) to give methyl trans-4-[[N-(2-furoyl)-N-[3-(dimethylamino)-propyl]amino]methylcyclohexanecarboxylate (102 mg, yield; 75%) as oil. MS (APCI) m/z; 373 [M+H]+
(4) The compound obtained in the above step (3) is treated in the same manner as described in Example 1 to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[N-(2-furoyl)-N-[3-(dimethylamino)propyl]aminomethyl]cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride as an amorphous powder. MS (APCI) m/z; 657 [M+H]+
The corresponding materials are treated in the same manner as described in Example 654 to the compounds as shown in the following Table 27.
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in either one of the aforementioned Example 404, 407, 617 and 652 to give the compounds as shown in the following Table 27.1.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr n-propyl group
*hydrochloride, **dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 1 to give the compounds as shown in the following Table 27.2.
**dihydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
(1) The compound obtained in Example 630 (1) (730 mg) and 4-piperidone monohydrate hydrochloride (1.05 g) are treated in the same manner as described in Example 630 (2) to give 1-(3-ethoxybenzyl)-4-[4-[[4-oxopiperidin-1-yl)carbonyl]-piperidin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (654 mg, yield: 97%) as an amorphous powder. MS (APCI) m/z: 464 [M+H]+
(2) The compound obtained in the above step (1) (50 mg) is treated in the same manner as described in Example 6 to give 1-(3-ethoxybenzyl)-4-[4-[[4-(dimethylamino)piperidin-1-yl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (15.6 mg, yield; 30%) as an amorphous powder. MS (APCI) m/z: 493 [M+H]+
(3) The compound obtained in the above step (2) (13.8 mg) is treated in the same manner as described in Example 4 (3) to give 1-(3-ethoxybenzyl)-4-[4-[[4-(dimethylamino)piperidin-1-yl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (15.9 mg, yield; 99.6%) as an amorphous powder. MS (APCI) m/z: 493 [M+H]+
The compound obtained in Example 441 (50 mg) is treated in the same manner as described in Example 6 to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(N-ethyl-N-n-propylamino)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (59.3 mg; yield; 64°/O) as an amorphous powder. MS (APCI) m/z: 534 [M+H]+
(1) To a solution of 4-fluorobenzenesulfonyl chloride (2.1 g) in methylene chloride (20 mL) is added pyridine (6 mL) and 1-(3-ethoxybenzyl)-4-piperazin-1-yl-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (3 g) under ice-cooling and the mixture is stirred at room temperature for 16 hours. The reaction mixture is diluted with chloroform (20 mL). Thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined, dried over anhydrous sodium sulfate and filtered. The filtrate is concentrated and the residue is purified by chromatography on silica gel (solvent; n-hexane:ethyl acetate=1:1→1:3) to give 1-(3-ethoxy-benzyl)-4-[4-(4-fluorobenesulfonyl)piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (3.2 g, yield; 88%) as an amorphous powder. MS (APCI) m/z: 497 [M+H]+
(2) The compound obtained in the above step (1) (100 mg) is treated in the same manner as described in Reference Example 1 (1) to give 1-(3-ethoxybenzyl)-4-[4-[4-[[2-(dimethylamino)ethyl]amino]benzenesulfonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (91 mg, yield; 80%) as an amorphous powder. MS (APCI) m/z: 565 [M+H]+
The compound obtained in Example 715 (2) (57 mg) is treated in the same manner as described in Example 416 and Example 4 (3) to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-acetyl-N-[2-(dimethylamino)ethyl]amino]benzenesulfonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (51 mg, yield; 75%) as an amorphous powder. MS (APCI) m/z: 607 [M+H]+
The compound obtained in Example 441 is treated in the same manner as described in Example 422 (2) and then the resultant product is treated in the same manner as described in Example 4 (3) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[N-(2-dimethylamino)ethyl-N-propyl]amino]cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (3.4 mg, yield; 6.5%) as an amorphous powder. MS (ESI) m/z: 577 [M+H]+
The corresponding materials are treated in the same manner as described in Example 713 to give 1-(3-ethoxybenzyl)-4-[4-[[4-[[2-(dimethylamino)ethyl]amino]-piperidin-1-yl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine. The product (53 mg) is treated in the same manner as described in Example 416 to give 1-(3-ethoxy-benzyl)-4-[4-[[4-[N-(cyclopropylcarbonyl)-N-[2-(dimethylamino)ethyl]amino]piperidin-1-yl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (23 mg, yield; 34%) as an amorphous powder. MS (APCI) m/z: 604 [M+H]+
(1) To a solution of 4-piperidinopiperidine (1.0 g) in methylene chloride (5 mL) and isopropanol (15 mL) is added diphenylcyanocarbonimide (1.56 g) and the mixture is stirred at room temperature for 24 hours. The reaction mixture is diluted with ethyl acetate (20 mL) and thereto is added a saturated sodium hydrogencarbonate solution (30 mL). After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined, washed with saturated brine, dried over anhydrous sodium sulfate and filtered. The filtrate is concentrated and the residue is purified by column chromatography on silica gel (solvent; chloroform:methanol=9:1→5:1) to give phenyl N-cyano-1,4-bipiperidine-1′-carboximidoate (2.5 g, yield; quantitative) as an amorphous powder. MS (APCI) m/z: 313 [M+H]+
(2) To the solution of the compound obtained in the above step (1) (70 mg) in tetrahydrofuran (3 mL) is added sodium hydride (14.4 mg) and 1-(2-fluoro-3-methyl-benzyl)-4-[4-(1-piperidyl)piperidin-1-yl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (60 mg) and the mixture is stirred at 100° C. for 24 hours. The reaction mixture is diluted with chloroform (3 mL) and thereto is added a saturated sodium hydrogencarbonate solution (5 mL). After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue is purified by column chromatography on silica gel (Purif 8, Hi-flash/M, solvent; chloroform:methanol=100:0→85:15) to give 1-(2-fluoro-3-methylbenzyl)-4-[4-[1,4′-bipiperidin-1′-yl(cyanoimino)methyl]-1H-pyrazolo[3,4-d]pyrimidine (77.4 mg, yield; 95%) as an amorphous powder. MS (APCI) m/z: 545 [M+H]+
(1) The compound obtained in Example 407 (282 mg) is treated in the same manner as described in Example 719 (1) to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-[(cyanoimino)(phenoxy)methyl]-N-ethyl]amino]methyl]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (221 mg, yield; 69%) as an amorphous powder. MS (APCI) m/z: 644 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (64 mg) in acetonitrile is added an aqueous dimethylamine solution (241 μL) and the mixture is stirred at 100° C. for 16 hours. After cooling, the reaction mixture is concentrated and the residue is purified by column chromatography on silica gel (Purif 8, Hi-flash/M, solvent; chloroform:methanol=100:0→90:10) to give 1-(3-ethoxybenzyl) [4-[[4[[N-[(cyanoimino)(dimethylamino)methyl]-N-ethyl]amino]methyl]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (51 mg, yield; 86%) as an amorphous powder. MS (APCI) m/z: 595 [M+H]+
(3) The compound obtained in the above step (2) (51 mg) is treated in the same manner as described in Example 4 (3) to give 1-(3-ethoxybenzyl)-4-[4-[4-[[N-[(cyanoimino)(dimethylamino)methyl]-N-ethyl]amino]methyl]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride (45.8 mg, yield; 80%) as an amorphous powder. MS (APCI) m/z: 595 [M+H]+
The corresponding materials are treated in the same manner as described in Example 366 to give 1-(3-ethoxybenzyl)-4-[4-[4-[2-(dimethylamino)ethylamino]-benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine. To a solution of the resultant product (50 mg) in methylene chloride/isopropanol (1:1, 1 mL) is added diphenyl cyanocarbonimidate (25 mg) and the mixture is stirred at 50° C. for 6 hours. The reaction mixture is diluted with chloroform (2 mL) and thereto is added a saturated sodium hydrogencarbonate solution (2 mL). After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined, dried over anhydrous sodium sulfate, filtered and concentrated. The residue is purified by column chromatography on silica gel (Purif 8, Hi-flash/M, solvent; chloroform:methanol=100:0→90:10) to give 1-(3-ethoxybenzyl)-4-[4-[4 [N-[2-(dimethylamino)ethyl]-N-[(2-propyloxy)(cyanoimino)methyl]amino]benzoyl]-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (44.3 mg, yield; 69%) as an amorphous powder. MS (APCI) m/z: 639 [M+H]+
(1) Cyclohexane-1,4-dicarboxylic acid monomethyl ester (1.0 g) is treated in the same manner as described in Example 3 to give 1-(3-ethoxybenzyl)-4[[[trans-4-(methoxycarbonyl)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine (1.83 g, yield; 81%) as an amorphous powder. MS (APCI) m/z: 507 [M+H]+
(2) The compound obtained in the above step (1) and N,N,N′-triethyl-1,2-ethylenediamine are treated in the same manner as described in Example 4 (2) and (3) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[N-ethyl-N-[2-(diethylamino)ethyl]carbamoyl]-cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine dihydrochloride as an amorphous powder. MS (ESI) m/z: 619 [M+H]+
Ethyl 4-(2-dimethylaminoethylamino)cyclohexanecarboxylate is treated in the same manner as described in Example 654 (3) and (4) to give 1-(6-propyl-2-pyridylmethyl)-4-[4-[[4-[N-naphthoyl-N-[2-(dimethylamino)ethyl]amino]cyclohexyl]-carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine as an amorphous powder. MS (ESI) m/z: 688 [M+H]+
(1) To a solution of ethyl 6-chloropyridazine-3-carboxylate (37 mg) in tetrahydrofuran (1.5 mL) is added N,N,N′-trimethylpropane-1,3-diamine (46 mg) and the mixture is stirred at room temperature overnight. The reaction mixture is diluted with tetrahydrofuran (1.5 mL) and thereto is added MP-Isocyanate (polystyrene methylisocyanate, 1.43 mmol/g, 400 mg). The mixture is shaken at room temperature overnight. The reaction mixture is filtered to remove the resin and the filtrate is extracted with chloroform/methanol. The organic layer is concentrated to give crude ethyl 6-[N-(3-dimethylaminopropyl)-methylamino]-3-pyridazinecarboxylate. MS (ESI) m/z: 267 [M+H]+
(2) The compound obtained in the above step (1) is treated in the same manner as described in Example 1 and Example 4 (3) to give 1-(3-ethoxybenzyl)-4-[4-[[6-[N-methyl-N-[3-(dimethylamino)propyl]amino]pyridazin-3-yl]carbonyl]piperazin-1-yl)-1H-pyrazolo[3,4-d]pyrimidine hydrochloride as an amorphous powder. MS (APCI) m/z: 559 [M+H]+
(1) To a solution of the compound obtained in Example 22 (500 mg) and N-benzyloxycarbonyl-β-alanine (291 mg) in methylene chloride (5 mL) is added diethyl cyanophosphonate (230 μL) and the mixture is stirred at room temperature overnight. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform. The organic layer is evaporated to remove solvent and the residue is purified by column chromatography on silica gel (solvent; chloroform/methanol=10:1) to 1-(3-ethoxybenzyl)-4-[4-[4-[N-[3-[N′-methyl-N′-(benzyloxycarbonyl)amino]propionyl]-N-[2-(dimethylamino)ethyl]amino]benzoyl]-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine give (431 mg, yield; 61%) as an yellow liquid. MA (APCI) m/z: 748 [M+H]+
(2) A suspension of the compound obtained in the above step (1) (480 mg), ammonium formate (81 mg) and 10% palladium-carbon (100 mg) in methanol (5 mL) is refluxed under heating for 2 hours. The reaction mixture is filtered and the filtrate is concentrated in vacuo. The residue is purified by column chromatography on silica gel (solvent; chloroform/methanol=5:1), and then the resultant product is treated in the same manner as described in Example 4 (3) to give 1-(3-ethoxybenzyl)-4-[4-[4-[N-[3-(methylamino)propionyl]-N-[2-(dimethylamino)ethyl]amino]benzoyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride (390 mg, yield; 99%) as a yellow liquid. MA (APCI) m/z: 614 [M+H]+
(1) Methyl 4-[trans-4-[(2-nitrophenyl)sulfonyl]piperazin-1-yl]cyclohexanecarboxylate (compound obtained in Reference Example 310, 1.29 g) treated in the same manner as described in Example 1 to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-[4-[(2-nitrophenyl)sulfonyl]piperazin-1-yl]cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo-[3,4-d]pyrimidin (1.91 g, yield; 85%) as an yellow liquid. MS (APCI) m/z: 718 [M+H]+
(2) A suspension of the compound obtained in the above step (1) (1.71 g), potassium carbonate (658 mg) and thiophenol (270 μL) in N,N-dimethylformamide (10 mL) is stirred at room temperature for 4 hours. The reaction mixture is diluted with water/ethyl acetate and the mixture is treated with cation-exchange resin (SCX-2, IST Ltd., solvent; methanol/water=1:1→1N ammonia/methanol) to give 1-(3-ethoxy-benzyl)-4-[trans-4-[[4-(1-piperazinyl)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo-[3,4-d]pyrimidin (1.09 g, yield; 86%) as a colorless solid. MS (APCI) m/z: 533
(3) To a solution of the compound obtained in the above step (2) (50 mg) in 1,2-dichloroethane (1 mL) is added successively formaldehyde solution (15 μL), acetic acid (11 μL) and sodium triacetoxyborohydride (30 mg) under ice-cooling and the mixture is stirred at room temperature overnight. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform. The organic layer is evaporated to remove solvent and the resultant crude product is purified by HPLC (solvent; 10 mM ammonium carbonate/methanol) to give 1-(3-ethoxybenzyl)-4-[4-[[trans-4-(4-methylpiperazin-1-yl)cyclohexyl]carbonyl]-piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidin (47 mg, yield; 92%) as a colorless solid. MS (APCI) m/z: 547 [M+H]+
To a solution of the compound obtained in Example 725 (50 mg) in methylene chloride (3 mL) is added ethyl chloroformate (10 μL) and triethylamine (22 μL) and the mixture is stirred at room temperature overnight. The reaction mixture is diluted with a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform. The organic layer is evaporated to remove solvent and the residue is purified by column chromatography on silica gel (solvent; chloroform/methanol=10:1), and the resultant product is treated in the same manner as described in Example 4 (3) to give 1-(3-ethoxybenzyl)-4-[4-[4-(N-[3-[N-methyl-N-(ethoxycarbonyl)amino]propionyl)-N-[2-(dimethylamino)ethyl]amino]benzoyl]piperazin-1-yl]-1H-pyrazolo [3,4-d]pyrimidine hydrochloride (17 mg, yield; 30%) as an amorphous powder. MS (APCI) m/z: 686 [M+H]+
The corresponding materials are treated in the same manner as described in either one of Examples 416, 714, 715, 716 and 717 to give the compounds as shown in the following Table 27.3.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in either one of Examples 1, 366 and 407 to give the compounds as shown in the following Table 27.4.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in either one of Examples 3, 407 and 434 to give the compounds as shown in the following Table 27.5.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in either one of Examples 713 and 718 to give the compounds as shown in the following Table 27.6.
*hydrochloride, **dihydrochloride
Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group, n-Bu: n-butyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in either one of Examples 720 and 724 to give the compounds as shown in the following Table 27.7.
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group
*hydrochloride
Me: methyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 1 to give the compounds as shown in the following Table 27.8.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
Me: methyl group, n-Pr: n-propyl group
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 3 to give the compound as shown in the following Table 27.9.
Me: methyl group
The corresponding materials are treated in the same manner as described in Example 723 to give the compounds as shown in the following Table 27.10.
**dihydrochloride
Me: methyl group, n-Pr: n-propyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 633 or 726 to give the compounds as shown in the following Table 27.11.
*hydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 366 to give the compounds as shown in the following Table 27.12.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, t-Bu: tert-butyl group
The corresponding materials are treated in the same manner as described in Example 719 to give the compounds as shown in the following Table 27.13.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 720 to give the compounds as shown in the following Table 27.14.
**dihydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 722 to give the compounds as shown in the following Table 27.15.
**dihydrochloride
Me: methyl group, Et: ethyl group
**dihydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 723 to give the compounds as shown in the following Table 27.16.
**dihydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, t-Bu: tert-butyl group
**dihydrochloride
Me: methyl group, n-Pr: n-propyl group, t-Bu: tert-butyl group
The corresponding materials are treated in the same manner as described in Example 727 to give the compounds as shown in the following Table 27.17.
*hydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 630 to give the compounds as shown in the following Table 27.18.
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 723 or 725 to give the compounds as shown in the following Table 27.19.
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 720 or 727 to give the compounds as shown in the following Table 27.20.
**dihydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 723 or 724 to give the compounds as shown in the following Table 27.21.
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride
Me: methyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 713 to give the compounds as shown in the following Table 27.22.
*hydrochloride
Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 713 to give the compounds as shown in the following Table 27.23.
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, t-Bu: tert-butyl group
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, t-Bu: tert-butyl group
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 718 to give the compounds as shown in the following Table 27.24.
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride,
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride, Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 1 to give the compounds as shown in the following Table 27.25.
Me: methyl group, Et: ethyl group, n-Pr n-propyl group
The corresponding materials are treated in the same manner as described in Example 654 to give the compounds as shown in the following Table 27.26.
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, t-Bu: tert-butyl group
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, t-Bu: tert-butyl group
*hydrochloride, Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
*hydrochloride, Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
The corresponding materials are treated in the same manner as described in Example 416 to give the compounds as shown in the following Table 27.27.
*hydrochloride, Me: methyl group, Et: ethyl group
The compound obtained in Example 726(2) (45 mg) is treated in the same manner as described in Example 416 to give 1-(3-ethoxybenzyl)-4-[trans-4-[[4-(4-acetyl-piperazin-1-yl)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine as an yellow oil. Subsequently, the compound is treated in the same manner as described in Example 4(3) to give 1-(3-ethoxybenzyl)-4-[trans-4-[[4-(4-acetyl-piperazin-1-yl)cyclohexyl]carbonyl]piperazin-1-yl]-1H-pyrazolo[3,4-d]pyrimidine hydrochloride (39.8 mg, yield: 78%) as an amorphous powder.
MS (APCI) 575 [M+H]+
The corresponding materials are treated in the same manner as described in Example 1075 to give the compounds as shown in the following Table 27.28.
*hydrochloride, Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 637 to give the compounds as shown in the following Table 27.29.
*hydrochloride, Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 407 to give the compounds as shown in the following Table 27.30.
*hydrochloride, Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 1 or 3 to give the compounds as shown in the following Table 27.31.
*hydrochloride, Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
The corresponding materials are treated in the same manner as described in Example 637 to give the compounds as shown in the following Table. 27.32.
*hydrochloride, Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 1 to give the compounds as shown in the following Table 27.33.
**dihydrochloride, Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 1 to give the compounds as shown in the following Table. 27.34.
*hydrochloride, Me: methyl group, Et: ethyl group
**dihydrochloride, Me: methyl group, Et: ethyl group
The corresponding materials are treated in the same manner as described in Example 1 or 637 to give the compounds as shown in the following Table. 27.35.
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
The corresponding materials are treated in the same manner as described in Example 723 to give the compounds as shown in the following Table. 27.36.
*hydrochloride, Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
(1) A solution of ethyl 4-fluorobenzoate (20 g), N,N-dimethylethylenediamine (20 g) and potassium carbonate (32.9 g) in dimethylsulfoxide (200 mL) is stirred at 80° C. for 3 days. After cooling to room temperature, to the reaction mixture is added ethyl acetate and water, and the mixture is stirred and extracted with ethyl acetate (×2). The organic layer is treated with 10% HCl to transfer basic materials to the aqueous layer. The aqueous layer is washed with ethyl acetate, neutralized with 10% sodium hydroxide solution and extracted with ethyl acetate (×3). The combined extract is dried over anhydrous sodium sulfate and concentrated in vacuo. The resultant crude product is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=8:1→4:1) to give ethyl 4-[[2-(dimethylamino)ethyl]amino]-benzoate (12.45 g, yield; 44%) as an oil. MS (APCI) m/z: 237 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (5 g) and pyridine (10 mL) in methylene chloride (20 mL) is added dropwise acryloyl chloride (2.55 mL) under ice-cooling and the mixture is stirred at room temperature for 3 hours. To the reaction mixture is added successively water and a saturated sodium hydrogencarbonate solution, and the mixture is stirred and extracted with chloroform. The extract is washed with a saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The resultant crude product is purified by flash column chromatography on silica gel (Solvent; Chloroform:methanol=1:0→50:1) to give ethyl 4-[N-acryloyl-N-[2-(dimethylamino)ethyl]amino]benzoate (1.62 g, yield; 26%) as a red oil.
(3) A mixture of the compound obtained in the above step (2) (1.62 g) and a 50% aqueous dimethylamine solution (5 mL) in acetonitrile (20 mL) is stirred at room temperature for 1.5 hours and the reaction mixture is concentrated in vacuo. The resultant crude product is purified by flash column chromatography on silica gel (Solvent; chloroform:methanol=1:0→50:1→chloroform:methanol:aqueous ammonia=25:1:0.1) to give ethyl 4-[N-[3-(dimethylamino)propionyl]-N-[2-(dimethylamino)ethyl]amino]benzoate (1.78 g, yield; 95%) as a red oil. MS (APCI) m/z; 336 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 1 to give the compounds as shown in the following Table 28.
Me: methyl group, Et: ethyl group, nPr: n-propyl group, Ph: phenyl group
To a solution of the compound obtained in Reference Example 1(1) (200 mg) and cyclopropanecarbonyl chloride (115 μL) in methylene chloride (4 mL) is added dropwise pyridine (137 μL) under ice-cooling and the mixture is stirred at room temperature for 23 hours. To the reaction mixture is added successively water and a saturated sodium hydrogencarbonate solution, and the mixture is stirred. The mixture is extracted with chloroform and the extract is concentrated. The resultant crude product is purified by flash column chromatography on NH-silica gel (Hi-Flash column; Yamazen, Solvent; chloroform:methanol=1:0→89:11) to give ethyl 4-[N-(cyclo-propylcarbonyl)-N-[2-(dimethylamino)ethyl]amino]benzoate (209 mg, yield; 81%) as a yellow oil. MS (APCI) m/z: 305 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 11 to give the compounds as shown in the following Table 29.
Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
Me: methyl group, Et: ethyl group, tBu: tert-butyl group
A solution of the compound obtained in Reference Example 1(1) (200 mg) and n-butyl isocyanate (143 μL) in methylene chloride (4 mL) is stirred at room temperature for 23 hours. To the reaction mixture is further added n-butyl isocyanate (143 μL) and the mixture is stirred at 50° C. for 17 hours. The reaction mixture is concentrated and the resultant crude product is purified by gel-permeation chromatography (JAI Gel-H column, Solvent; chloroform) to give ethyl 4-[N-(butylcarbamoyl)-N-[2-(dimethylamino)ethyl]amino]benzoate (179 mg, yield; 63%) as a yellow resin. MS (APCI) m/z: 336 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 27 to give the compounds as shown in the following Table 30.
Me: methyl group, Et: ethyl group
To a solution of ethyl 4-benzylaminobenzoate (1.5 g) in methylene chloride (15 mL) is added dropwise successively chloroacetyl chloride (0.56 mL) and N,N-diisopropylethylamine (1.54 mL) under ice-cooling, and the mixture is stirred for 1 hour. To the reaction mixture is added diethylamine (3 mL) at the same temperature and the mixture is stirred at room temperature for 12 hours. To the reaction mixture is added water (10 mL) and the organic layer is separated. The aqueous layer is extracted with chloroform (5 mL). The organic layers are combined and concentrated. The resultant crude product is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=6:1→3:1) to give ethyl 4-[N-(N′,N′-diethyl-glycyl)-N-benzylamino]benzoate (2.0 g, yield; 92%) as a brown oil. MS (APCI) m/z; 369 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 31 to give the compounds as shown in the following Table 31.
Me: methyl group, Et: ethyl group, Ph: phenyl group
(1) To a solution of methyl 4-formylbenzoate (25 g) in methylene chloride (250 mL) is added successively N,N-dimethylethylenediamine (67 g), acetic acid (87 mL), and sodium triacetoxyborohydride (50.6 g). The mixture is stirred at room temperature overnight. The reaction mixture is concentrated in vacuo and to the residue is added a saturated potassium carbonate solution. The mixture is extracted with ethyl acetate, and the organic layer is dried over anhydrous sodium sulfate and concentrated in vacuo to give methyl 4-[[[2-(dimethylamino)ethyl]amino]methyl]-benzoate (31.9 g, yield; 89%) as a colorless oil. MS (APCI) m/z: 237 [M+H]+
(2) To a solution of p-nitrophenyl chloroformate (18.1 g) in methylene chloride (300 mL) is added dropwise 3-(dimethylamino)propanol (14.2 mL) and the mixture is stirred at room temperature for 4 hours. To the mixture is added successively methyl 4-[[[2-(dimethylamino)ethyl]amino]methyl]benzoate (13.7 g) and triethylamine (25 mL) and the mixture is stirred at room temperature for one day. The reaction mixture is washed with a saturated potassium carbonate solution, dried over anhydrous sodium sulfate and concentrated in vacuo. The resultant crude product is purified by flash column chromatography on NH-silica gel (Chromatorex NH silica gel, Solvent; ethyl acetate:n-hexane=1:1) to give methyl 4-[[N-[2-(dimethylamino)ethyl]-N-[[3-(dimethylamino)propoxy]carbonyl]amino]methyl]benzoate (7.97 g, yield; 38%) as a colorless oil. MS (APCI) m/z: 366 [M+H]+
To a solution of the compound obtained in Reference Example 36(1) (200 mg) in methylene chloride (4 mL) is added propionyl chloride (110 μL) and thereto is added dropwise pyridine (137 μL) under ice-cooling. The mixture is stirred at room temperature for 4 hours. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution (10 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→90:10) to give methyl 4-[[N-propionyl-N-[2-(dimethylamino)ethyl]amino]methyl]benzoate (191 mg, yield; 78%) as an amorphous powder. MS (APCI) m/z: 293 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 37 to give the compounds as shown in the following Table 32.
Me: methyl group
To a solution of methyl 4-[[[2-(dimethylamino)ethyl]amino]methyl]benzoate (compound obtained in Reference Example 36(1), 200 mg) in methylene chloride (4 mL) is added n-butyl isocyanate (143 μL) and the mixture is stirred at room temperature for 4 hours. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution (10 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→90:10) to give methyl 4-[[N-(butylcarbamoyl)-N-[2-(dimethylamino)ethyl]amino]methyl]-benzoate (261 mg, yield; 92%) as a pale yellow oil. MS (APCI) m/z: 336 [M+H]+
To a solution of methyl 4-formylbenzoate (300 mg) in 1,2-dichloroethane (6 mL) is added diethylamine (113 μL), and thereto is added sodium triacetoxyborohydride (581 mg) and acetic acid (261 μL) under ice-cooling. The mixture is stirred at room temperature for 18 hours. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution (10 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=70:30→40:60) to give methyl 4-(diethylaminomethyl)benzoate (144 mg, yield; 60%) as an oil. MS (APCI) m/z: 222 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 44 to give the compounds as shown in the following Table 33.
Me: methyl group, Et: ethyl group
Me: methyl group
To a solution of ethyl 4-[[2-(dimethylamino)ethyl]amino]benzoate (compound obtained in Reference example 1(1), 200 mg) in 1,2-dichloroethane (4 mL) is added isobutylaldehyde (384 μL) and thereto is added sodium triacetoxyborohydride (448 mg) and acetic acid (145 μL) under ice-cooling. The mixture is stirred at room temperature for 24 hours. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution (10 mL). After stirring, the organic layer is separated and concentrated. The resultant crude product is purified by column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=80:20→50:50) to give ethyl 4-[N-[2-(dimethylamino)ethyl]-N-isobutylamino]benzoate (239 mg, yield; 97%) as a pale yellow oil. MS (APCI) m/z: 293 [M+]+
The corresponding materials are treated in the same manner as described in Reference Example 60 to give the compounds as shown in the following Table 34.
Me: methyl group, Et: ethyl group
To a solution of ethyl 4-[[2-(dimethylamino)ethyl]amino]benzoate (compound obtained in Reference Example 1(1), 3.0 g) in methylene chloride (30 mL) is added N-(tert-butoxycarbonyl)glycine (2.89 g) and thereto is added diethyl cyanophosphonate (2.89 mL) under ice-cooling. The mixture is stirred at room temperature for 24 hours. The reaction mixture is diluted with chloroform (20 mL) and thereto is added a saturated sodium hydrogencarbonate solution (50 mL). After, stirring, the organic layer is separated, washed with a saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo. The resultant crude product is purified successively by column chromatography on silica gel (Solvent; chloroform:methanol:28% aqueous ammonia=100:10:1) and gel-permeation chromatography (Nippon Bunseki Kogyo, Solvent; chloroform) to give ethyl 4-[N-[N′-(tert-butoxycarbonyl)glycyl]-N-[2-(dimethylamino)ethyl]amino]benzoate (4.2 g, yield; 84%) as a pale yellow crystals. MS (APCI) m/z: 394 [M+H]+
(1) To a solution of methyl 4-bromomethylbenzoate (22.8 g) in N,N-dimethylformamide (450 ml) is added dropwise 50% aqueous dimethylamine solution (27 mL) and the mixture is stirred at room temperature for 3 hours. The reaction mixture is poured into water with ice and extracted with ethyl acetate. The extract is washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The resultant crude product is purified by flash column chromatography on silica gel (Solvent; chloroform:methanol=10:1) to give methyl 4-[(dimethylamino) methyl]benzoate (27.5 g, yield; 99%) as brown oil. MS (APCI) m/z: 194 [M+H]+
(2) To the compound obtained in the above step (1) (19.2 g) is added conc. hydrochloric acid (66 mL) and water (167 mL) and the mixture is refluxed overnight. The reaction mixture is concentrated in vacuo. The resultant crude crystals are washed with ether to give 4-[(dimethylamino)methyl]benzoic acid hydrochloride (19.0 g, yield; 88%) as a colorless powder. MS (APCI) m/z: 179 [M+H]+
(1) To a solution of methyl 6-methylnicotinate (6.3 g) in carbon tetrachloride (100 mL) is added successively N-bromosuccinimide (8.9 g) and 2,2′-azobisisobutyronitrile (342 mg), and the mixture is refluxed under heating for 6 hours. After cooling to room temperature, to the reaction mixture is added n-hexane (300 mL) and the insoluble materials are removed by filtration. The filtrate is concentrated in vacuo and the resultant crude product is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=10:1) and stirred in n-hexane to give methyl 6-(bromomethyl)nicotinate (3.4 g, yield; 35%) as colorless crystals.
(2) To a solution of the compound obtained in the above step (1) (350 mg) in tetrahydrofuran (5 mL) is added 50% aqueous dimethylamine solution (3 mL) and the mixture is stirred at room temperature for 10 minutes. To the reaction mixture is added ethyl acetate. The mixture is washed successively with water and saturated brine, dried over anhydrous sodium sulfate and purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:1) to give methyl 6-(dimethylamino-methyl)nicotinate (276 mg, yield; 93%) as a colorless oil. MS (APCI) m/z: 195 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 69 to give the compounds as shown in the following Table 35.
Me: methyl group, Et: ethyl group
(1) To a solution of ethyl 4-hydroxybenzoate (15.3 g) in N,N-dimethylformamide (150 mL) is added successively 2-dimethylaminoethyl chloride hydrochloride (15.9 g) and potassium carbonate (43.2 g) and the mixture is stirred at 110° C. overnight. To the reaction mixture is added water and the mixture is extracted with ethyl acetate. The organic layer is washed successively with a saturated sodium hydrogencarbonate solution and water, dried over anhydrous sodium sulfate, and concentrated in vacuo. The resultant crude product is purified by flash column chromatography on silica gel (Solvent; chloroform:methanol=10:1) to give ethyl 4-[2-(dimethylamino)ethoxy]benzoate (14.1 g, yield; 65%) as a brown oil. MS (APCI) m/z: 238 [M+H]+
(2) To the compound obtained in the above step (1) (14.1 g) is added conc. hydrochloric acid (40 mL) and water (100 mL) and the mixture is refluxed overnight. The reaction mixture is concentrated in vacuo and the resultant crystals is washed with methanol/ether to give 4-[2-(dimethylamino)ethoxy]benzoic acid hydrochloride (14.1 g, yield; 56/o) as a brown powder. MS (APCI) m/z: 210 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 78 to give the compounds as shown in the following Table 36.
Me: methyl group
(1) Thionyl chloride (1.33 mL) is added dropwise to methanol (30 mL) under ice-cooling and the solution is stirred for 10 minutes. To the solution is added 4-hydroxycinnamic acid (3.0 g) and the mixture is stirred at room temperature for 4 days. The reaction mixture is concentrated and the residue is diluted with ethyl acetate (50 mL), washed successively with a saturated sodium hydrogencarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is re-crystallized from ethyl acetate/n-hexane to methyl 4-hydroxycinnamate (2.86 g, yield; 88%) as pale yellow crystals.
M.p. 136.5-137° C.
(2) To a solution of the compound obtained in the above step (1) (1.53 g) in N,N-dimethylformamide (25 mL) is added successively 3-chloropropyldimethylamine hydrochloride (1.69 g), potassium carbonate (3.12 g), and tetrabutylammonium iodide (105 mg). The mixture is stirred at 80° C. for 17 hours. After cooling, to the reaction mixture is added water (100 mL) and the mixture is extracted with ethyl acetate (150 mL×2). The organic layer is separated, washed successively with water (150 mL, ×2) and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo to give methyl trans-3-[4-[3-(dimethylamino)propyloxy]phenyl]acrylate (2.07 g, yield; 92%) as a pale yellow oil. MS (APCI) m/z: 264 [M+H]+
(3) To a solution of the compound obtained in the above step (2) (2.07 g) in methanol (15 mL) is added 1N sodium hydroxide solution (15 mL) and the mixture is stirred at room temperature for 15 hours. The reaction mixture is concentrated and the residue is neutralized (pH 7) with 1N HCl and thereto is added methanol. The mixture is stirred and the precipitated crystals are collected to give trans-3-[4-[3-(dimethylamino)propyloxy]phenyl]acrylic acid (1.93 g, yield; 98%) as colorless crystals. M.p. 191-193° C.
(1) To a solution of methyl 4-(aminomethyl)benzoate (5.08 g) in methylene chloride (30 mL) is added di-tert-butyl dicarbonate (6.4 g) under ice-cooling and the mixture is stirred at room temperature for 24 hours. The reaction mixture is diluted with methylene chloride (20 mL) and thereto is added water (40 mL). After stirring, the organic layer is separated and concentrated to give methyl 4-[N-(tert-butoxy-carbonyl)aminomethyl]benzoate.
(2) To a solution of the compound obtained in the above step (1) (1.5 g) in tetrahydrofuran (15 mL) and thereto is added sodium hydride (60% dispersion in mineral oil, 407 mg) under ice-cooling. The mixture is stirred at room temperature for 30 minutes. To the reaction mixture is added dropwise ethyl iodide (2.26 mL) under ice-cooling and the mixture is stirred at 60° C. for 1 hour. After cooling to room temperature, the reaction mixture is diluted with ethyl acetate (10 mL) and thereto is added water. After standing, the organic layer is separated and concentrated. The resultant product is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=20:1) to give methyl 4-[[N-ethyl-N-(tert-butoxycarbonyl)]-aminomethyl]benzoate (929 mg, yield; 56%) as an amorphous powder. MS (APCI) m/z: 294 [M+H]+
(1) To a solution of 4-hydroxybenzaldehyde (610 mg) in N,N-dimethylformamide (20 mL) is added successively 2-dimethylaminoethyl chloride hydrochloride (1.08 g) and potassium carbonate (2.0 g), and the mixture is stirred at room temperature for 18 hours. To the reaction mixture is added ethyl acetate, and the mixture is washed with water and saturated brine and dried over anhydrous sodium sulfate. The resultant crude product is purified by column chromatography in silica gel (Solvent; chloroform:methanol=20:1→10:1) to give 4-[2-(dimethylamino)-ethoxy]benzaldehyde (640 mg, yield; 66%) as a pale yellow oil. MS (APCI) m/z: 194 [M+H]+
(2) To a solution of tert-butyl dimethylphosphonoacetate (807 mg) in tetrahydrofuran (20 mL) is added sodium hydride (60% dispersion in mineral oil, 144 mg) under ice-cooling and the mixture is stirred for 30 minutes. To the reaction mixture is added a solution of the compound obtained in the above step (1) (580 mg) in tetrahydrofuran (10 mL) and the mixture is stirred at room temperature for 1 hour. To the reaction mixture is added ice-water. The mixture is extracted with ethyl acetate and the organic layer is washed with saturated brine and dried over anhydrous sodium sulfate. The resultant crude product is purified by column chromatography on silica gel (Solvent; chloroform:methanol=20:1) to give tert-butyl trans-3-[4-[2-(dimethylamino)ethoxy]phenyl]acrylate (920 mg, yield; quantitative) as colorless crystals. MS (APCI) m/z: 292 [M+H]+
(3) A solution of the compound obtained in the above step (2) (880 mg) in 4N HCl-dioxane (10 mL) is stirred at room temperature for 6 hours. The reaction mixture is diluted with diethylether, and the precipitated crystals are collected by filtration and washed with diethylether to give trans-3-[4-[2-(dimethylamino)ethoxy]phenyl]acrylic acid hydrochloride (750 mg, yield; 91%) as colorless crystals. MS (APCI) m/z: 236 [M+H]+
(1) Thionyl chloride (8.7 mL) is added dropwise to methanol (90 mL) at −30° C. under argon gas atmosphere over a period of 15 minutes and a mixture is stirred at −20° C. for 30 minutes. To the mixture is added 3-amino-4-methoxybenzoic acid (5.0 g) and the solution is stirred at the same temperature for 15 minutes and at room temperature for 3 days. The reaction mixture is concentrated in vacuo and the resultant crystals are washed with methanol/ether to give methyl 3-amino-4-methoxybenzoate hydrochloride (6.25 g, yield; 96%) as colorless crystals. M.p. 213-215° C., MS (APCI) m/z: 182 [M+H]+
(2) To a suspension of the compound obtained in the above step (1) (1.08 g) in tetrahydrofuran (10 mL) is added pyridine (2.4 mL) and methanesulfonyl chloride (0.56 mL) and the mixture is stirred at room temperature for 30 minutes. To the reaction mixture is added water and the mixture is stirred. The mixture is extracted with ethyl acetate. The extract is treated by column chromatography on diatomite and concentrated in vacuo. The resultant residue is recrystallized from ether/hexane to give methyl 3-methanesulfonylamino-4-methoxybenzoate (1.08 g, yield; 69%) as crystals. MS (APCI) m/z: 258 [M+H]+
(3) A solution of the compound obtained in the above step (2) (260 mg), potassium carbonate (222 mg), and 2-chloroethyldiethylamine hydrochloride (414 mg) in dimethylsulfoxide (2 mL) is vigorously stirred. To the reaction mixture is added water. After stirring, the mixture is extracted with ethyl acetate. The extract is washed with saturated brine, filtered through NH-silica gel column and concentrated in vacuo. The resultant residue is purified by flash column chromatography on NH-silica gel (HI-flash column, Solvent; chloroform:methanol=1:0→95:5) and treated with HCl/ethyl acetate to 3-[N-[2-(diethylamino)ethyl]-N-(methanesulfonyl)amino]4 methoxybenzoate hydrochloride (228 mg, yield; 93%) as colorless resin. MS (APCI) m/z: 359 [M+H]+
(1) To a solution of vanilin (76.1 g) in N,N-dimethylformamide (500 mL) is added successively 4-(2-chloroethyl)morpholine hydrochloride (130 g), sodium iodide (7.5 g), and potassium carbonate (207 g). The mixture is stirred at 80° C. for 15 hours. The reaction mixture is concentrated in vacuo. The residue is diluted with ethyl acetate, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. To the residue is added diisopropyl ether and the resultant crystals are collected by filtration to give 3-methoxy-4-(2-morpholinoethoxy)-benzaldehyde (112.6 g, yield; 85/o) as orange crystals. MS (APCI) m/z: 266 [M+H]+
(2) To a solution of triethyl phosphonoacetate (37.0 g) in tetrahydrofuran (150 mL) is added sodium hydride (60% dispersion in mineral oil, 6.4 g) with ice cooling and the mixture is stirred for 0.5 hour. To the mixture is added dropwise a solution of the compound obtained in the above step (1) (40.0 g) in tetrahydrofuan (140 mL) under ice-cooling and the mixture stirred at the same temperature for 2 hours. The reaction mixture is diluted with ethyl acetate (500 mL), washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. To the precipitated product is added diisopropyl ether and the resultant crystals are collected by filtration to give ethyl trans-3-[3-methoxy-4-(2-morpholinoethoxy)phenyl]acrylate (46.2 g, yield; 91%) as orange crystals. MS (APCI) m/z: 336 [M+H]+
(3) To a solution of the compound obtained in the above step (2) (43.3 g) in methanol (250 mL) is added 2N sodium hydroxide solution (100 mL) and the mixture is stirred at room temperature overnight. The reaction mixture is concentrated in vacuo and neutralized. The precipitated crystals are collected by filtration and dissolved in ethanol. The insoluble materials are removed by filtration and the resultant crystals are recrystallized to give trans-3-[3-methoxy-4-(2-morpholinoethoxy)phenyl]acrylic acid (34.8 g, yield; 88%) as yellow crystals. MS (APCI) m/z: 308 [M+H]+
(1) To methanol (1500 mL) is added dropwise thionyl chloride (254 mL) at −30° C. over a period of one hour and the mixture is stirred at room temperature for 0.5 hour. To the mixture is added trans-cyclohexane-1,4-dicarboxylic acid (500.0 g) and the mixture is stirred at room temperature for 17 hours. The reaction mixture is concentrated in vacuo and the residue is diluted with chloroform, washed with a saturated sodium hydrogencarbonate solution and saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is crystallized from n-hexane, collected by filtration, and dried to give dimethyl trans-cyclohexane-1,4-dicarboxylate (545.0 g). MS (APCI) m/z: 201 [M+]+
(2) To a solution of the compound obtained in the above step (1) (150.0 g) in tetrahydrofuran (1500 mL) is added dropwise a mixture of a solution of 28% sodium methoxide-methanol (149 g) and water (13.2 g) under ice-cooling. The mixture is stirred at room temperature for 3.5 hours and thereto is added n-hexane (150 mL). The resultant precipitate is collected by filtration and added to a mixture of conc hydrochloric acid (50 mL), water (450 mL) and chloroform (1000 ml). The mixture is stirred at room temperature for 20 minutes. The chloroform layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is crystallized from n-hexane, collected by filtration, and dried to give mono-methyl trans-cyclohexane-1,4-dicarboxylate (106.0 g). MS (ESI) m/z: 185 [M−H]−
(3) To a solution of the compound obtained in the above step (2) (100.0 g) in tert-butanol (1000 mL) is added diphenylphosphoryl azide (155 g) and triethylamine (78.6 mL). The mixture is stirred at 60° C. for 1 hour and refluxed under heating for 17 hours. After cooling, ice-cooled water is poured into the reaction mixture and the mixture is extracted with ethyl acetate. The organic layer is washed with a saturated sodium hydrogencarbonate solution and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is dissolved in methanol (250 mL) and thereto is added water (750 mL). The mixture is stirred for 0.5 hour under ice-cooling. The resultant precipitate is collected by filtration, washed with water/methanol (3:1, 1000 mL) and n-hexane, and dried over anhydrous sodium sulfate to give methyl trans-4-(tert-butoxycarbonylamino)cyclohexanecarboxylate (117.0 g). MS (APCI) m/z: 275 [M+NH4]+
(1) To a solution of the compound obtained in Reference Example 85 (234.0 g) in dioxane (500 mL) is added 4N HCl-dioxane (500 mL) and the mixture is stirred at room temperature for 19 hours. The reaction mixture is concentrated in vacuo and the residue is suspended in diethyl ether. The precipitate is collected by filtration to give methyl trans-4-aminocyclohexanecarboxylate hydrochloride (121.9 g). MS (APCI) m/z: 158 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (93.0 g) in methanol (1000 mL) is added 35% formaldehyde solution (95.4 mL), sodium acetate (39.4 g) and 10% palladium-carbon (10 g) and the mixture is stirred at room temperature under atmospheric pressure of hydrogen for 3.5 hours. The insoluble materials are removed through Celite and the filtrate is concentrated in vacuo. To the residue is added 20% potassium carbonate solution (500 mL) and the mixture is extracted with chloroform. The organic layer is dried over anhydrous sodium sulfate and potassium carbonate, and concentrated in vacuo. The residue is purified by column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=2:1) to give methyl trans-4-(dimethylamino)cyclohexanecarboxylate (87.3 g). MS (APCI) m/z: 186 [M+H]+
(3) To a solution of the compound obtained in the above step (2) (27.6 g) in dioxane (300 mL) and water (100 mL) is added 6N HCl (50 mL) and the mixture is refluxed under heating for 4 hours. To the reaction mixture is added 6N HCl (50 mL) and the mixture is further refluxed for 1 hour. The reaction mixture is concentrated in vacuo and the residue is subjected to azeotropic distillation with toluene. The residue is suspended in diisopropyl ether and the resultant precipitate is collected by filtration, washed with diisopropyl ether, and dried to give trans-4-(dimethylamino)cyclohexanecarboxylic acid (27.5 g). MS (APCI) m/z: 172 [M+H]+
(1) A suspension of the compound obtained in Reference Example 86(1) (10 g), 1,4-diiodobuthane (19.2 g), and sodium carbonate (16.4 g) in tetrahydrofuran (300 mL) and N,N-dimethylacetamide (60 mL) is stirred at 70° C. for 20 hours. The reaction mixture is concentrated in vacuo and the residue is dissolved in ethyl acetate/water. The organic layer is separated, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by column chromatography on NH-silica gel (Solvent; ethyl acetate:n-hexane=1:5) to give methyl trans-4-(1-pyrrolidinyl)cyclohexanecarboxylate (10.9 g). MS (APCI) m/z: 212 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (10.9 g) in dioxane (150 mL) is added 2N aqueous HCl (80 mL) and the mixture is stirred at 110° C. for 3 hours while evaporating to remove methanol. The reaction mixture is concentrated in vacuo. The residue is triturated with diethyl ether and collected by filtration to give trans-4-(1-pyrrolidinyl)cyclohexanecarboxylic acid hydrochloride (11.1 g). MS (APCI) m/z: 198 [M+H]+
(1) A suspension of the compound obtained in Reference Example 86(1) (47.5 g), bis(2-chloroethyl) ether (34.5 mL), sodium carbonate (77.9 g), and sodium iodide (88 g) in tetrahydrofuran (1400 mL) and N,N-dimethylacetamide (280 mL) is refluxed for 18 hours. To the reaction mixture is added bis(2-chloroethyl)ether (23 mL) and sodium iodide (22 g) and the mixture is refluxed for 6 hours. The reaction mixture is concentrated in vacuo and the residue is dissolved in ethyl acetate/water. The organic layer is separated, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by column chromatography on NH-silica gel (Solvent; ethyl acetate:n-hexane=1:30→1:5→1:3) to give methyl trans-4-morpholinocyclohexanecarboxylate (53.9 g). MS (APCI) m/z: 228 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (53.8 g) in dioxane (750 mL) is added 2N aqueous HCl (400 mL) and the mixture is stirred at 110° C. for 4 hours while evaporating to remove methanol. The reaction mixture is concentrated in vacuo. The residue is suspended in diethyl ether and collected by filtration to give trans-4-morpholinocyclohexanecarboxylic acid hydrochloride (54.8 g). MS (APCI) m/z: 214 [M+H]+
(1) To a suspension of trans-4-(aminomethyl)cyclohexanecarboxylic acid (6.29 g) in methanol (32 mL) is added dropwise thionyl chloride (6 mL) under ice-cooling and the mixture is stirred at room temperature overnight. The reaction mixture is concentrated in vacuo to give methyl trans-4-(aminomethyl)cyclohexanecarboxylate hydrochloride (8.69 g). MS (APCI) m/z: 172 [M+H]+
(2) To a suspension of the compound obtained in the above step (1) (8.69 g) in dichloromethane (400 mL) is added triethylamine (11.2 mL). After stirring at room temperature for a few minutes, to the reaction mixture is added 35% formaldehyde solution (15.9 mL) and sodium triacetoxyborohydride (25.43 g), and then the mixture is stirred at room temperature for 2 hours. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform. The organic layer is washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo to give methyl trans-4-(dimethylaminomethyl)-cyclohexanecarboxylate (7.42 g). MS (APCI) m/z: 200 [M+H]+
(3) To a solution of the compound obtained in the above step (2) (7.41 g) in dioxane (140 mL) is added 2N aqueous HCl (70 mL), and the mixture is refluxed under heating for 3 hours. After cooling, the reaction mixture is concentrated in vacuo. The residue is subjected to azeotropic distillation with toluene and dried to give trans-4-(dimethylaminomethyl)cyclohexanecarboxylic acid hydrochloride (8.45 g) as an amorphous powder. MS (APCI) m/z: 186 [M+H]+
(1) To a suspension of sodium hydride (60% dispersion in mineral oil, 33.6 g) in tetrahydrofuran (600 mL) is added dropwise a solution of triethyl phosphonoacetate (188.4 g) in tetrahydrofuran (100 mL) under ice-cooling. The mixture is stirred at the same temperature for 0.5 hour and thereto is added dropwise a solution of pyridine 4-carboxaldehyde (75.00 g) in tetrahydrofuran (100 mL). The mixture is stirred for 1 hour. To the reaction mixture is added ice water (1000 mL) and the mixture is extracted with ethyl acetate. The organic layer is washed with water, a saturated sodium hydrogencarbonate solution and saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is suspended in a small amount of diisopropyl ether under ice-cooling. The resultant precipitates are collected by filtration, washed with diisopropylether, and n-hexane and dried to give ethyl 3-(4-pyridyl)acrylate (77.53 g). MS (APCI) m/z: 178 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (28.00 g) in acetic acid (280 mL) is added platinum oxide (1.80 g), and the mixture is stirred at room temperature under hydrogen gas atmosphere for 24 hours. The reaction mixture is filtered to remove insoluble materials and the filtrate is concentrated in vacuo. The resultant residue is dissolved in dioxane (200 mL) and thereto is added 4N HCl-dioxane (200 mL). The mixture is concentrated in vacuo and the residue is suspended in diethyl ether/diisopropylether. The resultant precipitates are collected by filtration, washed with diisopropylether, and dried to give ethyl 3-(4-piperidyl)propionate hydrochloride (33.50 g). MS (APCI) m/z: 186 [M+H]+
To a solution of ethyl (4-pyridyl)acetate (50.00 g) in acetic acid (500 mL) is added platinum oxide (3.44 g), and the mixture is shaken at room temperature under hydrogen gas atmosphere for 20 hours. The reaction mixture is filtered to remove insoluble materials and the filtrate is concentrated in vacuo. The resultant residue is dissolved in dioxane (200 mL) and thereto is added 4N HCl-dioxane (400 mL). The mixture is concentrated in vacuo and the residue is suspended in diethyl ether/diisopropylether. The resultant precipitate is collected by filtration, washed with diisopropylether and dried to give ethyl (4-piperidyl)acetate hydrochloride (61.80 g). MS (APCI) m/z: 172 [M+H]+
(1) To a solution of the compound obtained in Reference Example 90 (70.83 g) in ethanol (700 mL) is added 2-iodopropane (38.2 mL) and potassium carbonate (132.3 g), and the mixture is refluxed under heating for 6 hours. The reaction mixture is filtered to remove insoluble materials and the filtrate is concentrated in vacuo. The residue is diluted with ethyl acetate (800 mL), washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=20:1→9:1) to give ethyl 3-(1-isopropylpiperidinyl)propionate (57.13 g). MS (APCI) m/z: 228 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (57.12 g) in dioxane (1200 mL) is added 2N HCl (600 mL) and the mixture is refluxed under heating for 3 hours. The reaction mixture is concentrated in vacuo and subjected to azeotropic distillation with dioxane. The resultant residue is triturated with diethyl ether/diisopropylether (1:1, 500 mL). The precipitates are collected by filtration, washed with diisopropylether and dried to give 3-(isopropylpiperidin-4-yl)propionic acid hydrochloride (55.36 g). MS (APCI) m/z: 200 [M+H]+
The corresponding materials are treated in the same manner as described in Reference Example 92(1) and (2) to give the compounds as shown in the following Table 37.
(1) A suspension of the compound obtained in Reference Example 91 (5.00 g), 4-chloropyridine hydrochloride (3.62 g), and triethylamine (10.01 mL) in xylene (130 mL) is refluxed under heating for 20 hours. After cooling, the reaction mixture is filtered to remove insoluble materials and the filtrate is concentrated in vacuo. The residue is diluted with chloroform, washed with water, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by column chromatography on NH-silica gel (Solvent; chloroform:ethyl acetate=4:1) to give ethyl [1-(4-pyridyl)piperidin-4-yl]acetate (4.15 g). MS (APCI) m/z: 249 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (4.15 g) in dioxane (200 mL) is added 1N aqueous HCl (70 mL), and the mixture is refluxed under heating for 4 hours. The reaction mixture is concentrated in vacuo and the residue is lyophilized to give [1-(4-pyridyl)piperidin-4-yl]acetic acid hydrochloride (3.90 g). MS (APCI) m/z: 221 [M+H]+
(1) A mixture of ethyl isonipecotate (1.57 g), propionaldehyde (1.0 g), and 10% palladium-carbon (500 mg) in methanol (20 mL) is vigorously stirred at room temperature under hydrogen gas atmosphere for 2 hours. After substituting the argon gas for hydrogen, the palladium-carbon is collected by filtration and washed with methanol (100 mL). The washing and the filtrate are combined and concentrated in vacuo. The residue is dissolved in ethanol (20 mL) and thereto is added 4N HCl-dioxane (20 mL). The mixture is concentrated in vacuo and the residue is crystallized from ethanol/ether to give ethyl 1-propylpiperidin-4-carboxylate hydrochloride (1.81 g, yield; 77%) as colorless crystals. M.p. 114-117° C.
(2) A mixture of the compound obtained in the above step (1) (1.79 g) and conc. hydrochloric acid (20 mL) is refluxed under heating for 2 hours. After cooling, the reaction mixture is concentrated in vacuo and ether is added to the residue. The mixture is stirred and the supernatant is separated. The ether layer of the supernatant is removed and the residue is crystallized from ethanol/ether to give 1-propylpiperidin-4-carboxylic acid hydrochloride (1.11 g, yield; 70) as colorless crystals. M.p. 222-224° C.
To a solution of 4-[[2-(dimethylamino)ethyl]amino]benzaldehyde (300 mg) in methylene chloride (4 mL) is added 2-methoxyethyl chloroformate (234 μL) and thereto is added dropwise pyridine (252 μL) under ice-cooling. The mixture is stirred at 50° C. for 2 days. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution (10 mL). After stirring, the organic layer is separated and concentrated. The residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→90:10) to give 4-[N-[(2-methoxyethoxy)carbonyl]-N-[2-(dimethylamino)ethyl]amino]benzaldehyde (290 mg, yield; 63%) as an amorphous powder. MS (APCI) m/z: 294 [M+H]+
A solution of mono-methyl terephthaloyl chloride (500 mg), N,N-dimethylethylenediamine (178 mg) and diisoprpylethylamine (650 mg) in methylene chloride (5.0 mL) is stirred at room temperature overnight. The reaction mixture is diluted with ethyl acetate and thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated and concentrated in vacuo. The residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=9:1) to give methyl 4-[[[2-(dimethylamino)-ethyl]amino]carbonyl]benzoate (355 mg, yield; 70%) as yellow crystals. MS (APCI) m/z: 251 [M+H]+
Under argon gas atmosphere, lithium aluminum hydride (1.19 g) is suspended in tetrahydrofuran (50 mL) and thereto is added dropwise a solution of ethyl (1-isopropylpiperidin-4-yl)acetate (compound obtained in Reference Example 93(1), 6.43 g) in tetrahydrofuran (50 mL) over a period of 15 minutes under ice-cooling. The mixture is stirred at room temperature for 1.5 hours. To the reaction mixture is added successively water (1.2 mL), 2N sodium hydroxide solution (2.4 mL), and water (2.4 mL) under ice-cooling. The mixture is stirred at room temperature for 2 hours. After addition of magnesium sulfate, the reaction mixture is stirred for 10 minutes and filtered. The filtrate is concentrated in vacuo and the residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=1:1) to give 2-(1-isopropylpiperidinyl)ethanol (5.09 g, yield; 99%) as a colorless oil. MS (APCI) m/z: 172 [M+H]+
Under argon gas atmosphere, lithium aluminum hydride (3.29 g) is suspended in tetrahydrofuran (160 mL) and thereto is added ethyl 1-isopropylpiperidin-4-carboxylate (compound obtained in Reference Example 94(1), 5.00 g) under ice-cooling. The mixture is stirred at room temperature for 14 hours. To the reaction mixture is added successively water (3.3 mL), 2N sodium hydroxide solution (6.6 mL) and water (6.6 mL) under ice-cooling. The mixture is stirred at room temperature for 1 hour. After addition of magnesium sulfate, the reaction mixture is stirred for 10 minutes and filtered. The filtrate is concentrated in vacuo and the residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=1:1) to give (1-isopropylpiperidin-4-yl)methanol (3.75 g, yield; 99%) as a colorless oil. MS (APCI) m/z: 158 [M+H]+
To a solution of 1-methyl-2-imidazolecarboxaldehyde (10 g) in a mixture of ethanol (80 mL) and tatrahydrofuran (80 mL) is added sodium borohydride (4.2 g) under ice-cooling and the mixture is stirred at room temperature for 2 hours. The reaction mixture is concentrated in vacuo and the residue is dissolved in chloroform, washed with a saturated sodium hydrogencarbonate solution, dried over anhydrous sodium sulfate, and concentrated in vacuo. To the residue is added diisopropylether and the resultant crystals are collected by filtration to give (1-methyl-1H-imidazole-2-yl)methanol (9.9 g, yield; 98%) as colorless crystals. MS (APCI) m/z: 113 [M+H]+
(1) To a solution of methyl 4-[[(tert-butoxycarbonyl)amino]methyl]benzoate (compound obtained in Reference Example 81(1), 2.0 g) in tetrahydrofuran (20 mL) is added sodium hydride (60% dispersion in mineral oil, 0.9 g) under ice-cooling. The mixture is stirred at room temperature for 30 minutes and then to the reaction mixture is added dropwise methyl iodide (2.3 mL). The mixture is stirred at room temperature for 3 hours. The reaction mixture is diluted with ethyl acetate, washed with water, and concentrated in vacuo to give methyl 4-[[N-methyl-N-(tert-butoxycarbonyl)amino]-methyl]benzoate (1.9 g, yield; 94%) as a yellow oil. MS (APCI) m/z: 280 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (1.5 g) in methanol (5.0 mL) is added 4N HCl-dioxane (10.0 mL) and the mixture is stirred at room temperature for 1 hour. The reaction mixture is diluted with ether and the precipitated crystals are collected by filtration to give methyl 4-[(methylamino)-methyl]benzoate (0.89 g, yield; 78%) as colorless crystals. MS (APCI) m/z: 180 [M+H]+
(3) A suspension of the compound obtained in the above step (2) (100 mg), 2-(diethylamino)ethyl chloride hydrochloride (160 mg), and potassium carbonate (260 mg) in N,N-dimethylformamide (3.0 mL) is stirred at 80° C. for 3 hours. The reaction mixture is diluted with water and extracted with ethyl acetate. The extract is concentrated in vacuo and the residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=10:1) to give methyl 4-[[N-methyl-N-[[2-(diethylamino)ethoxy]carbonyl]amino]methyl]benzoate (94 mg, yield; 64%) as a yellow oil. MS (APCI) m/z: 323 [M+H]+
(1) To a solution of methyl 5-aminofuran-2-carboxylate (1.0 g) and pyridine (1.2 mL) in methylene chloride (6.0 mL) is added dropwise cyclopropanecarbonyl chloride (0.78 mL) under ice-cooling and the mixture is stirred at room temperature for 2 hours. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform. The extract is dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=3:1) to give methyl 5-[(cyclopropylcarbonyl)amino]furan-2-carboxylate (0.82 g, yield; 56%) as colorless crystals. MS (APCI) m/z: 210 [M+H]+
(2) A suspension of the compound obtained in the above step (1) (200 mg), 2-(dimethylamino)ethyl chloride hydrochloride (275 mg) and potassium carbonate (530 mg) in N,N-dimethylformamide (3.0 mL) is stirred at 80° C. for 3 hours. The reaction mixture is diluted with water and extracted with ethyl acetate. The extract is concentrated in vacuo and the residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=10:1) to give methyl 5-[N-(cyclopropyl-carbonyl)-N-[2-(dimethylamino)ethyl]amino]furan-2-carboxylate (196 mg, yield; 73%) as a colorless oil. MS (APCI) m/z: 281 [M+H]+
(1) To a solution of 2-nitorthiophen-4-carboxylic acid (10 g) in ethanol (50 mL) is added thionyl chloride (5.0 mL) under ice-cooling and the mixture is stirred at room temperature overnight. The reaction mixture is concentrated in vacuo and to the residue is added a saturated sodium hydrogencarbonate solution. The mixture is extracted with ethyl acetate and the extract is dried over anhydrous sodium sulfate and concentrated in vacuo. To the residue is added iron powder (16 g), ammonium chloride (16 g), ethanol (100 mL) and water (100 mL) and the mixture is refluxed under heating for 2 hours. The reaction mixture is diluted with ethyl acetate and filtered to remove insoluble materials. The filtrate is washed with a saturated sodium hydrogencarbonate solution and saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo to give ethyl 5-aminothiophen-3-carboxylate (6.34 g, yield; 65%) as a brown oil. MS (APCI) m/z: 172 [M+H]+
(2) A solution of the compound obtained in the above step (1) (5.62 g), di-tert-butyl dicarbonate (15.8 g), pyridine (6.4 mL), and 4-(dimethylamino)pyridine (0.4 g) in methylene chloride (50 mL) is stirred at room temperature for 3 hours. The reaction mixture is washed with water, dried over anhydrous sodium sulfate, and concentrated in vacuo. To the residue is added cesium carbonate (22 g) and ethanol (50 mL), and the mixture is stirred at 80° C. overnight. The reaction mixture is filtered to remove insoluble materials and the filtrate is diluted with water. The resultant crystals are collected by filtration to give ethyl 5-[(tert-butoxycarbonyl)amino]thiophen-3-carboxylate (6.07 g, yield; 68%) as brown crystals. MS (APCI) m/z: 272 [M+H]+
(3) A suspension of the compound obtained in the above step (2) (1.5 g), 2-(dimethylamino)ethyl chloride hydrochloride (1.5 g), and potassium carbonate (3.0 g) in N,N-dimethylfromamide (15 mL) is stirred at 80° C. for 3 hours. The reaction mixture is diluted with water and extracted with ethyl acetate. The extract is concentrated in vacuo and the residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=50:1) to give ethyl 5-[N-(tert-butoxycarbonyl)-N-[2-(dimethylamino)ethyl]amino]thiophen-3-carboxylate (1.53 g, yield; 81%) as a brown oil. MS (APCI) m/z: 343 [M+H]+
(4) To the compound obtained in the above step (3) is added methanol (5.0 mL) and 4N HCl-dioxane (10.0 mL), and the mixture is stirred at room temperature for 3 hours. The reaction mixture is concentrated in vacuo, neutralized with a saturated sodium hydrogencarbonate solution, and extracted with chloroform. The extract is dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=10:1) to give ethyl 5-[[2-(dimethylamino)ethyl]amino]thiophen-3-carboxylate (0.75 g, yield; 56%) as a brown oil. MS (APCI) m/z: 243 [M+H]+
(5) To a solution of the compound obtained in the above step (4) (50 mg) and pyridine (33 mg) in methylene chloride is added dropwise acetyl chloride (24 mg) under ice-cooling and the mixture is stirred at room temperature overnight. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform. The extract is dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=1:1) to give ethyl 5-[N-acetyl-N-[2-(dimethylamino)ethyl]amino]thiophen-3-carboxylate (46 mg, yield; 79%) as a yellow oil. MS (APCI) m/z: 285 [M+H]+
A solution of the compound obtained in Reference Example 104(4) (50 mg) and n-butyl isocyanate (31 mg) in chloroform (3 mL) is stirred at 60° C. overnight. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and extracted with chloroform. The extract is dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=1:1) to give ethyl 5-[N-(butylcarbamoyl)-N-[2-(dimethylamino)ethyl]amino]thiophen-3-carboxylate (58 mg, yield; 82%) as a yellow oil. MS (APCI) m/z: 342 [M+H]+
To 5-(ethoxycarbonyl)thiophen-2-carboxylic acid (250 mg) is added successively methylene chloride (3.0 mL), N,N-dimethylethylenediamine (100 mg), 1-hydroxybenzotriazole (205 mg), triethylamine (610 mg), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (290 mg). The mixture is stirred at room temperature overnight. The reaction mixture is diluted with chloroform and thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated and concentrated in vacuo. The residue is purified by flash column chromatography on NH-silica gel (Solvent; ethyl acetate) to give ethyl 5-[[[2-(dimethylamino)ethyl]amino]carbonyl]thiophen-2-carboxylate (160 mg, yield; 53%) as brown crystals. MS (APCI) m/z: 271 [M+H]+
(1) A mixture of tert-butyl 4-fluorobenzoate (2.14 g) and 1-(2-aminoethyl)pyrrolidine (1 mL) is stirred at 120° C. for one day. After cooling, to the reaction mixture is added water and the mixture is extracted with ethyl acetate (×2). The extract is concentrated by centrifugal concentrator and the residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=8:1→4:1) to give tert-butyl 4-[[2-(1-pyrrolidinyl)ethyl]amino]benzoate (1.62 g, yield; 51%) as an oil. MS (APCI) m/z: 291 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (96 mg) and pyridine (67 μL) in chloroform (1 mL) is added acetyl chloride (47 μL) under ice-cooling and the mixture is stirred at room temperature for one day. The reaction mixture is diluted with chloroform and washed with a saturated sodium hydrogencarbonate solution. The washing is extracted with chloroform (×2), and the extracts are combined and concentrated by centrifugation. The residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=4:1→2:3) to give tert-butyl 4-[N-acetyl-N-[2-(1-pyrrolidinyl)ethyl]amino]benzoate (37 mg, yield; 33%) as a colorless oil. MS (APCI) m/z: 333 [M+H]+
(1) To a solution of oxalyl chloride (2.0 mL) in dichloromethane (120 mL) is added dropwise a solution of dimethylsulfoxide (3.3 mL) in dichloromethane (15 mL) under cooling with dry ice-acetone bath and the mixture is stirred at the same temperature for 10 minutes. To the reaction mixture is added dropwise a solution of the compound obtained in Reference Example 100 ((1-isopropylpiperidin-4-yl)methanol) (3.00 g) in dichloromethane (30 mL) over a period of 15 minutes. The mixture is stirred at the same temperature for 2 hours and thereto is added dropwise triethylamine (13.3 mL) over a period of 10 minutes. The mixture is stirred at room temperature for 1 hour, poured into a saturated sodium hydrogencarbonate solution and extracted with dichloromethane. The extract is concentrated in vacuo and the aqueous layer is extracted with ethyl acetate. The extract and the dichloromethane layer are combined, washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo to give crude 1-isopropylpiperidin-4-carboxaldehyde (1.96 g). MS (APCI) m/z: 156 [M+H]+
(2) To a solution of triethyl phosphonoacetate (7.96 g) in tetrahydrofuran (50 mL) is added portionwise sodium hydride (60% dispersion in mineral oil, 1.45 g) under ice-cooling and the mixture is stirred at the same temperature for 20 minutes. To the mixture is added a solution of the compound obtained in the above step (1) (5.03 g) in tetrahydrofuran (25 mL). The mixture is stirred at the same temperature for 3 hours and diluted with diethyl ether. After addition of water, the reaction mixture is extracted with ethyl acetate. The organic layer is washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=9:1) to give ethyl 3-(1-isopropylpiperidin-4-yl)acrylate (6.87 g). MS (APCI) m/z: 226 [M+H]+
(3) To a solution of the compound obtained in the above step (2) (1.01 g) in ethanol (20 mL) is added 2N sodium hydroxide solution (4.5 mL) and the mixture is stirred at room temperature for 24 hours. To the reaction mixture is added 2N HCl (9 mL) and the mixture is concentrated in vacuo. The residue is lyophilized to give 3-(1-isopropylpiperidin-4-yl)acrylic acid hydrochloride (1.43 g). MS (APCI) m/z: 198 [M+H]+
A mixture of ethyl p-aminobenzoate (25.2 g), triethyl orthoformate (80 g) and trifluoroacetic acid (0.2 mL) is refluxed under heating for 3 hours. After cooling, the reaction mixture is concentrated in vacuo and the residue is subjected to azeotropic distillation with ethanol. The residue is suspended in ethanol (400 mL) and thereto is added sodium borohydride (23 g), and the mixture is refluxed under heating for 3 hours. After cooling, to the reaction mixture is added ethyl acetate and water. The mixture is stirred at room temperature for 1 day. The organic layer is separated, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by flash column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=4:1) to give ethyl 4-(methylamino)benzoate (12.7 g, yield; 46%) as a pale yellow amorphous powder. MS (APCI) m/z: 180 [M+H]+
To a suspension of trans-4-(aminomethyl)cyclohexanecarboxylic acid (8.35 g) in dioxane (100 mL) is added water (50 mL) and 1N sodium hydroxide solution (50 mL), and then added dropwise di-tert-butyl dicarbonate (12.7 g) under ice-cooling. The mixture is stirred at room temperature for 4 hours. The reaction mixture is concentrated in vacuo and the residue is diluted with ethyl acetate and thereto is added an aqueous citric acid solution to acidify the solution (pH 3-4). The mixture is extracted with ethyl acetate, and the organic layer is washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is triturated with n-hexane, collected by filtration, and dried to give trans-4-(tert-butoxycarbonylaminomethyl)cyclohexanecarboxylic acid (13.30 g, yield; 97%) as crystals. MS (APCI) m/z: 256 [M−H]−
(1) To a solution of the compound obtained in Reference Example 85 (30.0 g) in N,N-dimethylformamide (150 mL) is added sodium hydride (60% dispersion in mineral oil, 5.60 g) under ice-cooling. The mixture is stirred at the same temperature for 0.5 hour and thereto is added successively methyl iodide (14.5 mL) and methanol (0.15 mL). The mixture is stirred at room temperature for 4 hours. To the reaction mixture is added a saturated ammonium chloride solution and ice water, and the mixture is extracted with ethyl acetate. The organic layer is washed with water and saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=10:1→7:1) to give methyl trans-4-[N-methyl-N-(tert-butoxycarbonyl)amino]cyclohexanecarboxylate (26.3 g, yield; 83%) as an amorphous powder. MS (APCI) m/z: 272 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (44.78 g) in methanol (300 mL) is added 2N sodium hydroxide solution (100 mL), and the mixture is stirred at room temperature for 6 hours. The reaction mixture is concentrated in vacuo and to the residue is added ice water, ethyl acetate, and 10% HCl. The mixture is extracted with ethyl acetate and the organic layer is washed with water and saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is suspended in a small amount of ethyl acetate and thereto is added n-hexane. The precipitated crystals are collected by filtration, washed with n-hexane-diisopropylether in several times and dried to give trans-4-[n-methyl-N-(tert-butoxycarbonyl)amino]-cyclohexanecarboxylic acid (39.20 g, yield; 92%) as crystals. MS (APCI) m/z: 256 [M−H]−
(1) 3-Methoxy-4-methylbenzoic acid (1 g) is treated in the same manner as described in Reference Example 69 to give methyl 3-methoxy-4-[(dimethylamino)-methyl]benzoate (792 mg, yield; 64%) as an oil. MS (APCI) m/z: 224 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (792 mg) in ethanol (10 mL) is added 2N sodium hydroxide solution (2.1 mL) and the mixture is stirred at 60° C. for 2 hours. After cooling, to the reaction mixture is added 2N HCl (4.3 mL). The mixture is concentrated in vacuo and to the residue is added methanol. The mixture is filtered to remove insoluble materials and the filtrate is concentrated in vacuo to give 3-methoxy-4-[(dimethylamino)methyl]benzoate hydrochloride (890 mg, yield; quantitative) as an amorphous powder. MS (APCI) m/z: 210 M+H]+
To a solution of the compound obtained in Reference Example 156 (200 mg) in dimethylformamide (2 mL) is added successively methylboronic acid (132 mg), [1,1-bis(diphenylphophino)ferrocene]dichloropalladium(II) complex with dichloromethane (60 mg) and potassium carbonate (305 mg). The mixture is stirred at 115° C. under argon gas atmosphere for 3 hours. The reaction mixture is diluted with ethyl acetate (10 mL) and thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated and concentrated by centrifugal concentrater. The residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→90:10) to give methyl 3-methyl-4-[(dimethylamino)methyl]-benzoate (65 mg, yield; 43%) as an amorphous powder. MS (APCI) m/z: 208 [M+H]+
To a suspension of cesium hydroxide (24.5 g) in dimethylformamide (500 mL) is added successively Molecular sieve 4A (44.5 g) and the compound obtained in Reference Example 86(1) (7.7 g), and the mixture is stirred at room temperature for 30 minutes. To the reaction mixture is added dimethylaminoethyl chloride hydrochloride (11 g), and the mixture is stirred for 22 hours. The reaction mixture is filtered to remove insoluble materials through Celite and washed with ethyl acetate. The filtrate and washing are combined and concentrated in vacuo. The residue is dissolved in chloroform (100 mL). To the solution is added a solution of di-tert-butyl dicarbonate (16 g) in chloroform (10 mL) under ice-cooling and the mixture is stirred at room temperature for 1.5 hours. The reaction mixture is washed with a saturated sodium hydrogencarbonate solution, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→50:1) to give methyl trans-4-[N-(tert-butoxy-carbonyl)-N-[2-(dimethylamino)ethyl]amino]cyclohexanecarboxylate (1.7 g, yield; 11%) as an amorphous powder. MS (APCI) m/z: 329 [M+H]+
To a suspension of the compound obtained in Reference Example 90 (500 mg) in dichloromethane (10 mL) is added diethylketone (388 μL), triethylamine (471 μL) and sodium triacetoxyborohydride (956 mg) under ice-cooling, and the mixture is stirred at room temperature overnight. The reaction mixture is diluted with chloroform and thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→90:10) to give ethyl 3-[1-(3-pentyl)piperidin-4-yl]propionate (130 mg). MS (APCI) m/z: 256 [M+H]+
The corresponding materials are treated in the same manner as described in either one of the aforementioned Reference Examples 1 to 115 to give the compounds as shown in the following Table 38.
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group
c-Pr: cyclopropyl group, n-Bu: n-butyl group
Me: methyl group
Me: methyl group
Me: methyl group
Me: methyl group
Me: methyl group, Ph: phenyl group
Me: methyl group
Me: methyl group
Me: methyl group
Et: ethyl group, i-Pr: isopropyl group, n-Pr: n-propyl group, n-Bu: n-butyl group
Me: methyl group, n-Bu: n-butyl group
Me: methyl group
Me: methyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
(1) To a solution of 1-(carbamoyl)-1-cyclopropanecarboxylic acid (500 mg) in tetrahydrofuran (2.0 mL) is added dropwise 2M boran-dimethylsulfide complex solution in tetrahydrofuran (7.5 mL) and the mixture is refluxed under heating for 4 hours. To the reaction mixture is added 10% HCl and the mixture is refluxed under heating for 30 minutes. After cooling, the reaction mixture is neutralized with a saturated sodium hydrogencarbonate solution, and thereto is added dioxane (10 mL) and di-tert-butyl dicarbonate (1.3 g). The mixture is stirred at room temperature for 3 hours. The reaction mixture is extracted with ethyl acetate and the extract is concentrated in vacuo. The residue is purified by flash column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=3:1) to give 1-hydroxymethyl-1-(tert-butoxycarbonylamiomethyl)cyclopropane (337 mg, yield; 45%) as a yellow oil. MS (APCI) m/z; 202 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (337 mg), methyl 4-hidroxybenzoate (280 mg), and triphenylphosphine (650 mg) in tetrahydrofuran (3.0 mL) is added dropwise diisopropyl azodicarboxylate (510 mg) under ice-cooling and the mixture is stirred at room temperature overnight. The reaction mixture is concentrated in vacuo and the residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=3:2) to give methy 4-[[1-(tert-butoxycarbonylamiomethyl)cyclopropyl]methoxy]benzoate (515 mg, yield; 92%) as a yellow oil. MS (APCI) m/z; 353 [M+H]+
A suspension of tert-butyl 4-fluorobenzoate (300 mg), 2-(dimethylamino)-ethanethiol hydrochloride (420 mg), and potassium carbonate (620 mg) in dimethylsulfoxide (3.0 mL) is stirred at 80° C. for 4 hours. The reaction mixture is diluted with water and extracted with ethyl acetate. The extract is concentrated in vacuo and to the residue is added 4N HCl-dioxane (1.5 mL). The mixture is stirred at room temperature overnight. The reaction mixture is diluted with ether and the precipitated crystals are collected by filtration to give 4-[2-(dimethylamino)ethylthio]-benzoic acid (380 mg, yield; 99%) as colorless crystals. MS (APCI) m/z; 226 [M+H]+
To a solution of ethyl 4-cyclohexanonecarboxylate (24 g) in methanol (200 mL) is added sodium borohydride (2.67 g) at −78° C. and the mixture is stirred at the same temperature for 1 hour. To the reaction mixture is added water, and the mixture is evaporated to remove methanol and extracted with ethyl acetate. The organic layer is washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. To a solution of the residue (20 g) in methylene chloride (200 mL) is added dropwise diisopropylethylamine (50 mL) and chloromethylmethylether (16 mL) under ice-cooling and the mixture is stirred at room temperature overnight. To the reaction mixture is added water and the mixture is extracted with ethyl acetate. The extract is concentrated and the residue is purified by flash column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=15:1) to give ethyl trans-4-(methoxymethoxy)-cyclohexanecarboxylate (12.3 g, yield; 40%) as a colorless oil. MS (APCI) m/z; 234 [M+H]+
The compound obtained in Reference Example 85(2) (mono-methyl trans-cyclohexane-1,4-dicarboxylate, 14.3 g) is dissolved in tetrahydrofuran (78 mL) and 1.0M boran-tetrahydrofuran complex solution in tetrahydrofuran (100 mL) is added dropwise thereto at −50° C. under argon gas atmosphere over a period of 1 hour. The mixture is stirred at −10° C. for 1 hour. To the reaction mixture is added water (160 mL) and a saturated sodium hydrogencarbonate solution (160 mL) under ice-cooling and the mixture is extracted with ethyl acetate (×4). The extract is washed with saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by flash column chromatography on silica gel (Solvent; chloroform:methanol=20:1) to give methyl trans-4-(hydroxymethyl)cyclohexanecarboxylate (13.25 g, yield; quantitative) as an oil. MS (APCI) m/z; 173 [M+H]+
(1) To a solution of methyl trans-4-(aminomethyl)cyclohexanecarboxylate hydrochloride (5.86 g) and triethylamine (12 mL) in chloroform (100 mL) is added dropwise acetyl chloride (2.69 g) under ice-cooling and the mixture is stirred at room temperature for 2 hours. The reaction mixture is poured into water and the mixture is extracted with chloroform (×2). The combined extracts are washed successively with 10% HCl, saturated brine, a saturated sodium hydrogencarbonate solution, and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by flash column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=1:1→0:1) to give methyl trans-4-(acetylaminomethyl)cyclohexanecarboxylate (5.92 g, yield; 98%) as an amorphous powder. MS (APCI) m/z; 214 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (4.32 g) in dimethylformamide (50 mL) is added successively sodium hydride (60% dispersion in mineral oil, 1.0 g), methyl iodide (5.91 g) and methanol (5 drops) under ice-cooling and the mixture is stirred at room temperature for 8 hours. After addition of sodium hydride (60% dispersion in mineral oil, 430 mg), methyl iodide (1.5 mL), and methanol (2 drops). The mixture is stirred for 2 hours. The reaction mixture is poured into water and extracted with ethyl acetate (×3). The extract is washed with water (×2), dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by flash column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=1:2→0:1) to give methyl trans-4-(N-methyl-N-acetylaminomethyl)cyclohexanecarboxylate (3.04 g, yield; 66%) as an oil. MS (APCI) m/z; 228 [+H]+
(3) The compound obtained in the above step (2) (3.03 g) is hydrolyzed by 2N sodium hydroxide solution (14 mL) in methanol/tetrahydrofuran and the reaction mixture is concentrated in vacuo and nuetralized with diluted aqueous HCl. The mixture is extracted with chloroform and the extract is dried over anhydrous sodium sulfate and concentrated in vacuo to give a crude carboxylic acid compound (2.67 g, 94%). The compound (500 mg) is refluxed under heating in 5N HCl for 2 days and the reaction mixture is concentrated to give a crude amino acid compound. To the compound is added a mixture of 1N sodium hydroxide solution (6 mL) and dioxane (4 mL) and di-tert-butyl dicarbonate (1.0 g). The mixture is stirred at room temperature for 12 hours. The reaction mixture is diluted with water and washed with ether. After neutralizing the aqueous layer with 10% citric acid solution, the mixture is extracted with ethyl acetate. The extract is washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo to give trans-4-[N-methyl-N-(tert-butoxycarbonyl)aminomethyl]cyclohexanecarboxylic acid (680 mg, yield; 90%) as an oil. MS (ESI) m/z; 270 [M−H]−
The compound obtained in Reference Example 238 (500 mg) and isopropyl-(2-methoxyethyl)amine (689 mg) are treated in the same manner as described in Example 654(1) and (2) to give methyl trans-4-[N-isopropyl-N-(2-methoxyethyl)aminomethyl]-cyclohexanecarboxylate (445 mg, yield; 56%) as oil. MS (APCI) m/z; 272 [M+H]+
(1) To a solution of ethyl 4-benzylaminobenzoate (6.0 g) in methylene chloride (60 mL) is added dropwise chlorobutyryl chloride (3.95 mL) and N,N-diisopropylethylamine (6.14 mL) under ice-cooling, and the mixture is stirred for 12 hours. The reaction mixture is diluted with chloroform and thereto is added 10% HCl. The organic layer is separated, washed successively with water, a saturated sodium hydrogencarbonate solution, water, and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is purified by flash column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=4:1) to give ethyl 4-[N-benzyl-N-(4-chlorobutyryl)amino]benzoate (8.27 g, yield; quantitative) as an oil. MS (APCI) m/z; 360/362 [M+H]+
(2) A solution of the compound obtained in the above step (1) (1.0 g), diethylamine (287 μL), and sodium iodide (417 mg) in dimethylsulfoxide (2.5 mL) is stirred at 100° C. under argon gas atmosphere for 1 day. After cooling, to the reaction mixture is added a saturated sodium hydrogencarbonate solution and the mixture is extracted with chloroform (×2). The extract is concentrated in vacuo and the residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=4:1) to give ethyl 4-[N-benzyl-N-[4-(diethylamino)butyryl]amino]benzoate (280 mg, yield; 25%) as an oil. MS (APCI) m/z; 397 [M+H]+
To a solution of ethyl 3,4-dihydroxybenzoate (500 mg), N,N-dimethylethanol-amine (565 μL), and triphenylphosphine (1.51 g) in methylene chloride (20 mL) is added dropwise diisopropyl azodicarboxylate (1.37 mL) under argon gas atmosphere and ice-cooling. The mixture is stirred at room temperature for 1 day. The reaction mixture is diluted with ethyl acetate, washed with water and treated with 10% HCl to transfer basic materials in ethyl acetate layer to the aqueous layer. The aqueous layer is neutralized with saturated aqueous sodium hydrogencarbonate and extracted with chloroform/methanol/tetrahydrofuran (4:1:1, ×2). The extract is dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by flash column chromatography on NH-silica gel (Solvent; n-hexane:ethyl acetate=1:1→chloroform:methanol=19:1) to give ethyl 4-[2-(dimethylamino)ethoxy]-3-hydroxy-benzoate (240 mg, yield; 35%) as oil. MS (APCI) m/z; 254 [M+H]+
To a solution of ethyl 4-aminobenzoate (10 g) and 4-(dimethylamino)benzaldehyde (9.0 g) in methylene chloride (200 mL) is added sodium triacetoxyborohydride (18.9 g) and acetic acid (3.47 mL) under ice-cooling, and the mixture is stirred at room temperature for 2 days. To the reaction mixture is added a saturated sodium hydrogencarbonate solution and extracted with chloroform (×2). The extract is concentrated in vacuo and the residue is triturated in n-hexane/ethyl acetate to give ethyl 4-[4-(dimethylamino)benzylamino]benzoate (11.6 g, yield; 64%) as powder. MS (APCI) m/z; 299 [M+H]+
(1) A solution of 4-(bromomethyl)benzaldehyde (15.9 g) and (1-ethoxy-carbonylethylidene)triphenylphosphorane (29 g) in chloroform (290 mL) is refluxed under heating for 12 hours. After cooling, the reaction mixture is concentrated and the residue is purified by column chromatography on silica gel (Solvent; chloroform) to give ethyl 3-[4-(bromomethyl)phenyl]-2-methylacrylate (18 mg, yield; 79%) as an oil.
(2) To a solution of pyrrolidine (0.89 g) and potassium carbonate (1.38 g) in water (10 mL) is added dropwise a solution of the compound obtained in the above step (1) (2.83 g) in tetrahydrofuran (5 mL) and the mixture is stirred at room temperature overnight. The reaction mixture is diluted with ethyl acetate, washed with water, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue is dissolved in ether and filtered to remove insoluble materials. To the filtrate is added oxalic acid (0.9 g) and the resultant precipitate is collected by filtration to give ethyl 2-methyl-3-[4-[(1-pyrrolidinyl)methyl]phenyl]acrylate hemioxalate (2.67 g, yield; 83%) as an amorphous powder.
(3) The compound obtained in the above step (2) (2.2 g) is treated with ethyl acetate/10% potassium carbonate solution and the ethyl acetate layer is concentrated in vacuo. The residue (2-methyl-3-[4-pyrrolidin-1-yl)methylphenyl]acrylic acid) is dissolved in methanol (10 mL) and thereto is added potassium hydroxide (1.38 g). The mixture is stirred at 50° C. for 5 hours. After cooling, to the reaction mixture is added ethanol (5 mL) and the resultant crystals are collected by filtration to give potassium 2-methyl-3-[4-[(1-pyrrolidinyl)methyl]phenyl]acrylate (1.65 g, yield; 83%) as a powder. M.p. 268° C. (dec.)
(1) To a solution of methyl 4-(bromomethyl)benzoate (500 mg) in tetrahydro-furan (5 mL) is added cyclopentylamine (929 mg) and the mixture is stirred at room temperature for 3 hours. The reaction mixture is diluted with chloroform (5 mL) and thereto is added a saturated sodium hydrogencarbonate solution. After stirring, the organic layer is separated, dried over anhydrous sodium sulfate, and filtered. The filtrate is concentrated and the residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=9:1→5:5) to give methyl 4-(cyclopentyl-aminomethyl)benzoate (469 mg, yield; 92%) as a colorless oil. MS (APCI) m/z; 234 [M+H]+
(2) The compound obtained in the above step (1) (480 mg) is treated in the same manner as described in Reference Example 89(2) to give methyl 4-[(N-cyclo-pentyl-N-methylamino)methyl]benzoate (420 mg, yield; 83%) as a colorless oil. MS (APCI) m/z; 248 [M+H]+
(1) The corresponding materials are treated in the same manner as described in Reference Example 107(1) to give tert-butyl 4-[[3-(dimethylamino)-2,2-dimethyl-propyl]amino]benzoate.
(2) The compound obtained in the above step (1) (500 mg) is treated in the same manner as described in Reference Example 89(2) to give tert-butyl 4-[N-[3-(dimethylamino)-2,2-dimethylpropyl]-N-ethylamino]benzoate (468 mg, yield; 85%) as a colorless oil. MS (APCI) m/z; 335 [M+H]+
The compound obtained in Reference Example 86(1) (1.00 g) is treated in the same manner as described in Reference Example 89(2) and the resultant product is treated in the same manner as described in Reference Example 81(1) to give methyl trans-4-[N-(tert-butoxycarbonyl)-N-ethylamino]cyclohexanecarboxylate (258.4 mg, yield; 14%) as a colorless oil. MS (APCI) m/z; 303 [M+NH4]+
(1) To a solution of malononitrile (28.2 g) in methanol (30 mL) is added a suspension of acetoin (25 g) in methanol (30 mL) and thereto is added dropwise diethylamine (11 mL) under ice-cooling (internal temp.: 10° C.). The mixture is stirred at room temperature for 3 hours. The reaction mixture is poured into ice water (300 mL) and the precipitated crystals are collected by filtration to give 2-amino-4,5-dimethyl-3-furonitrile (28.91 g, yield; 75%) as colorless crystals.
(2) To a solution of the compound obtained in the above step (1) (13.6 g) in methylene chloride (200 mL) is added piperidine (18.9 g) and thereto is added dropwise a solution of ethyl chlorooxoacetate (16.3 g) in methylene chlorode (200 mL) under ice-cooling. The mixture is stirred at 0° C. for 1.5 hours. The reaction mixture is diluted with chloroform (100 mL) and thereto is added water (300 mL). After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined and washed with saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate is concentrated and the residue is purified by column chromatography on silica gel (Solvent; chloroform:ethyl acetate=9:1) to give ethyl [(3-cyano-4,5-dimethylfuran-2-yl)amino](oxo)acetate (17 g, yield; 72%) as a colorless powder. A mixture of formic acid (30 mL) and acetic anhydride (30 mL) is stirred at 60° C. under argon gas atmosphere for 2 hours and thereto is added ethyl [(3-cyano-4,5-dimethylfuran-2-yl)amino](oxo)acetate (7.07 g). The mixture is refluxed for 14 hours. The reaction mixture is concentrated and the residue is purified by column chromatography on silica gel (Solvent; chloroform:ethyl acetate=9:1→chloroform:methanol=19:1) to give ethyl 5,6-dimethyl-4-oxo-3,4-dihydrofuro[2,3-d]pyrimidine-2-carboxylate (4.30 g, yield; 61%) as a colorless powder. M.p. 180-182° C.
(3) The compound obtained in the above step (2) (2.36 g) is dissolved in N,N-dimethylformamide (60 mL) and thereto is added dimethyaminoethyl chloride hydrochloride (2.16 g) and potassium carbonate (4.14 g). The mixture is stirred at 60° C. for 14 hours. The reaction mixture is diluted with chloroform (50 mL) and thereto is added water (70 mL). After stirring, the organic layer is separated and the aqueous layer is extracted with chloroform. The organic layers are combined and washed with saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate is concentrated and the residue is purified by column chromatography on silica gel (Solvent; chloroform:ethyl acetate:methanol=6:2:1). The resultant product is treated in the same manner as described in Example 5(2) to give ethyl 4-[2-(dimethylamino)ethoxy]-5,6-dimethylfuro[2,3-d]pyrimidine-2-carboxylate fumarate (780 mg, yield; 18%) as a colorless powder. M.p. 173-174° C.
(4) The compound obtained in the above step (3) (1.44 g) is dissolved in ethanol (20 mL) and thereto is added 2N sodium hydroxide solution (2.34 mL). The mixture is stirred at room temperature for 2 hours. The reaction mixture is concentrated and to the residue is added diethyl ether. The resultant crystals are collected by filtration and purified by reverse phase column chromatography (HP-20 column, Solvent; water:methanol=1:0→1:1) to give 4-[2-(dimethylamino)-ethoxy]-5,6-dimethylfuro[2,3-d]pyrimidine-2-carboxylic acid (970 mg, yield; 74%) as a colorless powder. M.p. 179-180° C.
(1) To a solution of 5-hydroxy-2-(hydroxymethyl)-4H-pyranone (1 g) in N,N-dimethylformamide (20 mL) is added potassium carbonate (1.95 g) and tert-butyl bromoacetate (1.37 g) and the mixture is stirred at room temperature for 16 hours. The reaction mixture is diluted with tetrahydrofuran (10 mL), and thereto is added water and saturated brine. After stirring, the organic layer is separated and the aqueous layer is extracted with tetrahydrofuran. The organic layers are combined and washed with saturated brine, dried over anhydrous sodium sulfate, and filtered. The filtrate is concentrated and the residue is washed with diethyl ether and collected by filtration to give tert-butyl [[6-(hydroxymethyl)-4-oxo-4H-pyran-3-yl]oxy]acetate (1.49 g, yield; 88%) as a colorless powder. MS (ESI) m/z; 257 [M+H]+, M.p. 90-92° C.
(2) To a solution of the compound obtained in the above step (1) (50 g) in methylene chloride (600 mL) is added Dess-Martin periodinate (1,1,1-tris(acetyloxy)-1,1-dihydro-1,2-benziodoxol-3(1H)-one, 91.04 g) and the mixture is stirred at room temperature for 5 days. The reaction mixture is filtered through Celite, and the filtrate is extracted with ethyl acetate and concentrated. The residue is washed with diethyl ether and collected by filtration to give tert-butyl [(6-formyl-4-oxo-4H-pyran-3-yl)-oxy]acetate (17.1 g, 34.5%) as a brown powder.
(3) The compound (2.0 g) obtained in the above step (2) is treated in the same manner as described in Example 6 to give tert-butyl [[4-oxo-6-[(1-pyrrolidinyl)methyl]-4H-pyran-3-yl]oxy]acetate (1.06 g, yield; 44%) as a yellow powder. MS (ESI) m/z; 316 [M+H]+, M.p. 93-94° C.
(4) To a solution of the compound obtained in the above step (3) (700 mg) in methylene chloride (5 mL) is added trifluoroacetic acid (2.5 mL) at 0° C. and the mixture is stirred at room temperature for 24 hours. The reaction mixture is concentrated and thereto is added ethyl acetate and water. After stirring, the aqueous layer is separated and washed with ethyl acetate again. The aqueous layer is lyophilized to give [[4-oxo-6-[(1-pyrrolidinyl)methyl]-4H-pyran-3-yl]oxy]acetic acid (820 mg, yield; 99%) as a colorless powder. MS (ESI) m/z; 254 [M+H]+
(1) To a solution of tert-butyl [[4-oxo-6-[(1-pyrrolidinyl)methyl]-4H-pyran-3-yl]oxy]acetate (compound obtained in Reference Example 249(2), 12 g) in toluene is added (1-methoxycarbonylmethylidene)triphenylphosphoran (17.36 g) and the mixture is stirred at 60° C. for 3 hours. The reaction mixture is concentrated and the residue is purified by flash column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=1:1) to give tert-butyl [2-[(1E)-2-(methoxycarbonyl)vinyl]-4-oxo-4H-pyran-3-yl]-oxyacetate (10.99 g, yield; 75%) as colorless crystals. MS (ESI) m/z; 311 [M+H]+, M.p. 115-116° C.
(2) To a solution of the compound obtained in the above step (1) (8.83 g) in tetrahydrofuran (100 mL) is added water (100 mL) and 2N sodium hydroxide solution (20.1 mL) and the mixture is stirred at room temperature for 4 hours. After addition of HCl (23 mL), the reaction mixture is concentrated and the residue (1.0 g) is dissolved in N,N-dimethylformamide (7 mL). To the solution is added N-methylmorpholine (3 mL) and polyphosphoric acid (3.9 mL) and the mixture is stirred at room temperature for 4 days. The reaction mixture is diluted with ethyl acetate and thereto is added a saturated sodium hydrogencarbonate solution and saturated brine. After stirring, the organic layer is separated and the aqueous layer is extracted with ethyl acetate. The organic layers are combined, washed with saturated brine, dried over anhydrous magnesium sulfate and filtered. The filtrate is concentrated and the residue is purified by flash column chromatography on silica gel (Solvent; chloroform:methanol=19:1) to give tert-butyl [2-[(1E)-2-[(4-methylpiperazin-1-yl)carbonyl]vinyl])oxo-4H-pyran-3-yl]oxyacetate (450 mg, yield; 35%) as a yellow crystals. MS (ESI) m/z; 379 [M+H]+, M.p. 124-125° C.
(3) To a solution of the compound obtained in the above step (2) (390 mg) in methylene chloride (5 mL) is added trifluoroacetic acid (2 mL) at 0° C. and the mixture is stirred at room temperature for 5 hours. The reaction mixture is concentrated and thereto is added water. The solution is lyophilized to give [2-[(1E)-2-[(4-methylpiperazin-1-yl)carbonyl]vinyl]-4-oxo-4H-pyran-3-yl]oxyacetic acid (330 mg, yield; 73%) as a colorless powder. MS (ESI) m/z; 323 [M+H]+
(1) Tert butyl 4-fluoro-3-methylbenzoate (4 g) is treated in the same manner as described in Reference Example 1(1) to give tert-butyl 4-[[2-(dimethylamino)ethyl]-amino]-3-methylbenzoate (1.3 g, yield; 24%) as an oil. MS (APCI) m/z; 279 [M+H]+
(2) To a solution of the compound obtained in the above step (1) (1.3 g) in dioxane (5 mL) is added 4N HCl-dioxane (5 mL) and 5N HCl (3 mL) and the mixture is stirred at 60° C. for 10 minutes. After cooling, the reaction mixture is concentrated in vacuo, and the residue is dissolved in water and lyophilized to give 4-[[2-(dimethylamino)ethyl]amino]-3-methylbenzoate dihydrochloride (1.35 g, yield; 98%) as an amorphous powder. MS (APCI) m/z; 223 [M+H]+
(1) A mixture of ethyl 4,4,4-trifluoroacetoacetate (23.1 g), urea (7.54 g) and ethyl orthoformate (18.6 g) is stirred at 130° C. under argon gas atmosphere for 1.5 hours. To the reaction mixture is added xylene (100 mL) and the mixture is stirred at 140° C. for 17 hours. The reaction mixture is evaporated to remove solvent and the residue is diluted with methanol. The solution is treated with an activated carbon powder, concentrated in vacuo, and triturated in diisopropylether to give ethyl 2-hydroxy-4-trifluoromethyl-5-pyrimidinecarboxylate (20.3 g, yield; 69%) as crystals. MS (ESI) m/z; 235 [M−H]−
(2) A solution of the compound obtained in the above step (1) (5.0 g) in phosphorus oxychloride (30 mL) is refluxed under heating for 2 hours. The reaction mixture is poured into water with ice and extracted with chloroform. The extract is washed with saturated brine, dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=5:1) to give ethyl 2-chloro-4-trifluoromethyl-5-pyrimidinecarboxylate (4.3 g, yield; 80%) as an oil. MS (GC-EI) m/z; 254 [M+]
(3) To a solution of the compound obtained in the above step (2) (500 mg) in tetrahydrofuran (3 mL) is added a solution of N,N,N′-trimethylethylenediamine (421 mg) in tetrahydrofuran (2 mL) under argon gas atmosphere and the mixture is stirred at room temperature for 1 hour. The reaction mixture is diluted with ethyl acetate, washed successively with a saturated sodium hydrogencarbonate solution, water, and saturated brine. The organic layer is dried over anhydrous sodium sulfate and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→50:1) to give ethyl 2-[N-methyl-N-[2-(dimethylamino)ethyl]amino]-4-trifluoromethyl-5-pyrimidinecarboxylate (540 mg, yield; 77%) as an oil. MS (APCI) m/z; 321 [M+H]+
To a solution of the compound obtained in Reference Example 86(1) (500 mg) in 1,2-dichloroethane (10 mL) is added triethylamine (665 μL), propionaldehyde (688 μL), sodium triacetoxyborohydride (1.68 g), and acetic acid (546 μL) under ice-cooling. The mixture is stirred at room temperature for 3 days. The reaction mixture is diluted with chloroform and thereto is added a saturated sodium hydrogencarbonate solution. The mixture is extracted with chloroform and the extract is concentrated in vacuo. The residue is dissolved in chloroform (6 mL) and thereto is added a solution of di-tert-butyl dicarbonate (690 mg) in chloroform (2 mL) under ice-cooling, and the mixture is stirred at room temperature for 2 hours. The reaction mixture is concentrated in vacuo and the residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→90:10) to give methyl trans-4-(dipropylamino)cyclohexanecarboxylate (600 mg, yield; 78%) as an oil. MS (APCI) m/z; 242 [M+H]+
(1) To a solution of methyl 4-(3-hydroxy-1-propinyl)benzoate (2 g) in methanol (32 mL) is added Lindlar catalyst (238 mg) and the mixture is stirred under hydrogen gas atmosphere at room temperature for 18 hours. To the reaction mixture is added Lindlar catalyst (90 mg) and the mixture is stirred for 1 hour. The reaction mixture is filtered to remove insoluble materials through Celite. The filtrate is concentrated in vacuo and the residue is purified by column chromatography on silica gel (Solvent; n-hexane:ethyl acetate=100:0→50:50) to give methyl 4-(3-hydroxy-1-propenyl)-benzoate (1.5 g, yield; 75%) as an amorphous powder. MS (APCI) m/z; 175 [M+H−H2O]+
(2) To a solution of the compound obtained in the above step (1) (200 mg) in tetrahydrofuran (4 mL) is added triethylamine (218 μL) and methanesulfonyl chloride (105 μL) under ice-cooling and the mixture is stirred for 30 minutes. The reaction mixture is diluted with ethyl acetate and thereto is added 10% citric acid solution. The mixture is extracted with ethyl acetate and the extract is washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. To the residue is dissolved in dimethylformamide (2 mL) and thereto is added 2M dimethylamine/tetrahydrofuran (2 mL). The mixture is stirred at room temperature for 2 days. The reaction mixture is diluted with ethyl acetate, washed with a saturated sodium hydrogencarbonate solution, and concentrated in vacuo. The residue purified by column chromatography on silica gel (Solvent; chloroform methanol=100:0→90:10) to give methyl 4-[3-(dimethylamino)-1-propenyl]-benzoate (198 mg, yield; 87%) as an oil. MS (APCI) m/z; 220 [M+H]+
To a solution of methyl 4-(3-tosyloxypropyl)benzoate (500 mg) in dimethylformamide (3 mL) is added 2M dimethylamine solution in tetrahydrofuran (2.2 mL) and the mixture is stirred at 50° C. for 15 hours. The reaction mixture is diluted with ethyl acetate, washed with a saturated sodium hydrogencarbonate solution and concentrated in vacuo. The residue is purified by column chromatography on silica gel (Solvent; chloroform:methanol=100:0→90:10) to give methyl 4-[3-(dimethylamino)-propyl]benzoate (357 mg, yield; quantitative) as an oil. MS (APCI) m/z; 222 [M+H]+
Methyl 4-(3-hydroxy-1-propynyl)benzoate is treated in the same manner as described in Reference Example 254(2) to give methyl 4-[3-(1-pyrrolidinyl)-1-propynyl]benzoate (463 mg, yield; 91%) as an oil. MS (APCI) m/z; 244 [M+H]+
Tert-butyl 4-fluorobenzoate is treated in the same manner as described in Reference Example 251 to give 4-[2-(dimethylamino)ethyl]aminobenzoic acid dihydrochloride as an amorphous powder. MS (APCI) m/z; 209 [M+H]+
The corresponding materials are treated in the same manner as described in either one of the aforementioned Reference Examples to give the compounds as shown in the following Table 39.
*hydrochloride
Me: methyl group, Et: ethyl group, n-Pr: n-propyl group, Boc: tert-butoxycarbonyl group
Me: methyl group
*: hydrochloride
Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
Me: methyl group, n-Pr: n-propyl group, i-Bu: isobutyl group, t-Bu: tert-butyl group
Me: methyl group, Et: ethyl group, Bzl: benzyl group, Boc: tert-butoxycarbonyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
(1) To a solution of 6-oxo-1,4,5,6-tetrahydropyridazine-3-carboxylic acid (20 g) in acetic acid (50 mL) is added 25% hydrobromic acid/acetic acid (200 mL) and thereto is added dropwise dimethylsulfoxide (22.0 g) at room temperature. The mixture is stirred at room temperature for 3 days. The resultant precipitates are collected by filtration, washed successively with acetic acid and water and dried at 40° C. overnight to give 6-oxo-1,6-dihydropyridazine-3-carboxylic acid (10.55 g, yield; 54%) as colorless crystals. MS (ESI) m/z: 139 [M−H]−
(2) To a solution of the compound obtained in the above step (1) (1.0 g) in toluene (5 mL) is added thionyl chloride (3 mL) and two drops of dimethylformamide under ice-cooling, and the mixture is stirred at 80° C. for 3 hours. After cooling, the reaction mixture is concentrated and to the residue is added ether. The resultant crystals are collected by filtration, washed with ether and dried to give ethyl 6-chloro-1,6-dihydropyridazine-3-carboxylate (813 mg, yield; 61%) as a colorless powder. MS (APCI) m/z: 187/189 [M+H]+
To a solution of ethyl 4-cyclohexanonecarboxylate (5 g), N,N-dimethyl-ethylene-1,2-diamine (6.45 mL) and acetic acid (3.36 mL) in chloroform (50 mL) is added sodium triacetoxyborohydride (9.8 g) under ice-cooling and the mixture is stirred at room temperature overnight. To the reaction mixture is added a saturated sodium hydrogencarbonate solution. After stirring, the mixture is basified with potassium carbonate and extracted with chloroform (×2). The extract is dried and concentrated in vacuo. The resultant crude product is purified by flash column chromatography on silica gel (Biotage, Flash 75M, solvent; chloroform/methano-1/aqueous ammonia=19:1:0.3) to give ethyl 4-(2-dimethylaminoethylamino)cyclohexanecarboxylate (a mixture of cis- and trans-isomer, 6.63 g, yield; 94%) as an oil. MS (APCI) m/z: 243 [M+H]+
The corresponding compounds are treated in the same manner as described in Reference Example 242 to give ethyl 4-(3-dimethylamino-2,2-dimethylpropoxy)-3-hydroxybenzoate (MS (APCI) m/z: 296 [M+H]+). To a solution of the compound (80 mg) in dimethylformamide (1.5 mL) is added sodium hydride (60% dispersion in mineral oil, 22.7 mg) and the mixture is stirred for 20 minutes. To the mixture is added 4-(2-chloroethyl)morpholine hydrochloride (55.4 mg) and the mixture is stirred at room temperature for one day. The reaction mixture is diluted with water and extracted with chloroform (×3). The extract is concentrated in vacuo and the resultant product is purified by flash column chromatography on NH-silica gel (solvent; ethyl acetate/n-hexane=5:95→25:75) to give ethyl 4-(3-dimethylamino-2,2-dimethyl-propoxy)-3-(2-morpholin-4-iyethoxy)benzoate (53 mg, yield; 70%) as an oil. MS (APCI) m/z: 409 [M+H]+
To a solution of methyl 6-chloronicotinate (10 g) in N,N-dimethylsulfoxide (15 mL) is added N,N-dimethylethylenediamine (10 mL) and the mixture is stirred at 75° C. overnight. The reaction mixture is diluted with water and extracted with ethyl acetate. The extract is concentrated in vacuo and the resultant product is purified by flash column chromatography on NH-silica gel (solvent; n-hexane/ethyl acetate=1:1) to give methyl 6-[(2-dimethlamino)ethyl]aminonicotinate (4.09 g, yield; 31%) as a yellow liquid. MS (APCI) m/z: 224 [M+H]+
(1) To a suspension of 1-amino-2-methyl-2-propanethiol hydrochloride (75 mg) in N,N-dimethylformamide (2 mL) is added sodium hydride (60% dispersion in mineral oil, 50 mg) and the mixture is stirred at room temperature for 30 minutes. To the mixture is added tert-butyl 4-fluorobenzoate (50 mg) and the mixture is stirred at 70° C. for 4 hours. The reaction mixture is diluted with water and extracted with ethyl acetate. The extract is concentrated in vacuo and the resultant product is purified by flash column chromatography on NH-silica gel (solvent; ethyl acetate) to give tert-butyl 4-[(2-amino-1,1-dimethylethyl)thio]benzoate (54 mg, yield; 75%) as a colorless oil. MS (APCI) m/z: 282 [M+H]+
(2) The compound obtained in the above step (1) (59 mg) is treated in the same manner as described in Example 618 (1) to give tert-butyl 4-[(2-dimethylamino-1,1-dimethylethyl)thio]benzoate (40 mg, yield; 62%) as a yellow oil. MS (APCI) m/z: 310 [M+H]+
(1) To a solution of 4-mercaptobenzoic acid (1.5 g) in methanol (20 mL) is added conc. sulfuric acid (0.5 mL) and the mixture is refluxed under heating overnight. The reaction mixture is evaporated to remove solvent and the residue is neutralized with a saturated sodium hydrogencarbonate solution. The mixture is extracted with ethyl acetate and the extract is concentrated in vacuo to give methyl 4-mercaptobenzoate (1.6 g, yield; 99%) as a colorless oil. MS (APCI) m/z: 167 [M−H]−
(2) The compound obtained in the above step (1) (50 mg) is treated in the same manner as described in Example 381 to give methyl 4-[(3-dimethylamino-2,2-dimethylpropyl)thio]benzoate (58 mg, yield; 69%) as a brown oil. MS (APCI) m/z: 282 [M+H]+
(1) To a solution of diethanolamine (5.0 g) and sodium carbonate (5.5 g) in water (25 mL) is added portionwise 2-nitrobenezenesulfonyl chloride (10.5 g) at 65° C. and the mixture is stirred at 90° C. for 3 hours. The reaction mixture is diluted with water and extracted with ethyl acetate. The extract is concentrated in vacuo and to the residue is added thionyl chloride (10 mL). The mixture is stirred at 90° C. for 2 hours. The reaction mixture is poured into water and the mixture is extracted with chloroform. The extract is concentrated in vacuo and the residue is purified by flash column chromatography on silica gel (solvent; n-hexane/ethyl acetate=4:1) to give N,N-di(2-chloroethyl)-2-nitrobenzenesulfonamide (7.1 g, yield; 46%) as a yellow liquid. MS (APCI) m/z: 327/329 [M+H]+
(2) The compound obtained in the above step (1) (2.0 g) and methyl trans-4-aminocyclohexanecarboxylate (1.0 g) are treated in the same manner as described in Example 434 (1) to give methyl trans-4-[4-(2-nitrobenzenesulfonyl)piperazin-1-yl]-cyclohexanecarboxylate (1.29 g, yield; 61%) as a yellow oil. MS (APCI) m/z: 412 [M+H]+
Methyl trans-4-[N-(tert-butoxycarbonyl)-N-[2-(dimethylamino)ethyl]amino]-cyclohexanecarboxylate (compound obtained in Reference Example 114, 2.0 g) is treated in the same manner as described in Example 404 (2) to give methyl trans-4-[N-[2-(dimethylamino)ethyl]amino]cyclohexanecarboxylate (1.5 g, yield; 82%) as colorless crystals. MS (APCI) m/z: 229 [M+H]+
The corresponding compounds are treated in the same manner as described in Reference Example 256 to give methyl 4-[3-(methylamino)-1-propynyl]benzoate. To a solution of the compound (227 mg) in chloroform (4 mL) is added di-tert-butyl dicarbonate under ice-cooling and the mixture is stirred at room temperature overnight. The reaction mixture is concentrated in vacuo and the resultant product is purified by column chromatography on silica gel (solvent; n-hexane/ethyl acetate=8:1) to give methyl 4-[3-(N-methy-N-tert-butoxycarbonylamino)-1-propynyl]benzoate (225 mg, yield; 86%) as colorless crystals. MS (APCI) m/z: 321 [+NH4]+
The corresponding compounds are treated in the same manner as described in Reference Example 254 to give methyl 4-[3-(methylamino)-cis-1-propenyl]benzoate and then the compound (135 mg) is treated in the same manner as described in Reference Example 312 to give methyl 4-[3-(N-methy-N-tert-butoxycarbonylamino)-1-propenyl]benzoate (135 mg, yield; 86%) as colorless crystals. MS (APCI) m/z: 323 [M+NH4]+
The corresponding compounds are treated in the same manner as described in Reference Example 255 to give methyl 4-[3-(methylamino)propyl]benzoate and then the compound (117 mg) is treated in the same manner as described in Reference Example 312 to give methyl 4-[3-(N-methy-N-tert-butoxycarbonylamino)propyl]-benzoate (123 mg, yield; 90%) as colorless crystals. MS (APCI) m/z: 325 [M+NH4]+
The corresponding materials are treated in the same manner as described in either one of the aforementioned Reference Examples to give the compounds as shown in the following Table 40.
Me: methyl group, Boc: tert-butoxycarbonyl group
Me: methyl group
Me: methyl group, Et: ethyl group
Me: methyl group, Et: ethyl group
Me: methyl group
Me: methyl group, Et: ethyl group
Me: methyl group, t-Bu: tert-butyl group
The corresponding materials are treated in the same manner as described in either one of the aforementioned Reference Examples to give the compounds as shown in the following Table 41.
Me: methyl group, Et: ethyl group, t-Bu: tert-butyl group
| Number | Date | Country | Kind |
|---|---|---|---|
| 2003-016770 | Jan 2003 | JP | national |
| 2003-205341 | Aug 2003 | JP | national |
| 2003-385399 | Nov 2003 | JP | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/JP04/00617 | 1/23/2004 | WO | 7/13/2005 |
