Patent Application
20090029955
The present invention relates to new therapeutically useful pyridothienopyrimidine derivatives, to processes for their preparation and to pharmaceutical compositions containing them. These compounds are potent and selective inhibitors of phosphodiesterase 4 (PDE4) and are thus useful in the treatment, prevention or suppression of pathological conditions, diseases and disorders known to be susceptible of being improved by inhibition of PDE4.
Phosphodiesterases (PDEs) comprise a superfamily of enzymes responsible for the hydrolysis and inactivation of the second messengers cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). Eleven different PDE families have been identified to date (PDE1 to PDE11) which differ in substrate preference, catalytic activity, sensitivity to endogenous activators and inhibitors, and encoding genes.
The PDE4 isoenzyme family exhibits a high affinity for cyclic AMP but has weak affinity for cyclic GMP. Increased cyclic AMP levels caused by PDE4 inhibition are associated with the suppression of cell activation in a wide range of inflammatory and immune cells, including lymphocytes, macrophages, basophils, neutrophils, and eosinophils. Moreover, PDE4 inhibition decreases the release of the cytokine Tumor Necrosis Factor α (TNFα). The biology of PDE4 is described in several recent reviews, for example M. D. Houslay, Prog. Nucleic Acid Res. Mol. Biol. 2001, 69, 249-315; J. E. Souness et al. Immunopharmacol. 2000 47, 127-162; or M. Conti and S. L. Jin, Prog. Nucleic Acid Res. Mol. Biol. 1999, 63, 1-38.
In view of these physiological effects, PDE4 inhibitors of varied chemical structures have been recentity disclosed for the treatment or prevention of chronic and acute inflammatory diseases and of other pathological conditions, diseases and disorders known to be susceptible to amelioration by inhibition of PDE4. See, for example, U.S. Pat. No. 5,449,686, U.S. Pat. No. 5,710,170, WO 98/45268, WO 99/06404, WO 01/57025, WO 01/57036, WO 01/46184, WO 97/05105, WO 96/40636, U.S. Pat. No. 5,786,354, U.S. Pat. No. 5,773,467, U.S. Pat. No. 5,753,666, U.S. Pat. No. 5,728,712, U.S. Pat. No. 5,693,659, U.S. Pat. No. 5,679,696, U.S. Pat. No. 5,596,013, U.S. Pat. No. 5,541,219, U.S. Pat. No. 5,508,300, U.S. Pat. No. 5,502,072 or H. J. Dyke and J. G. Montana, Exp. Opin. Invest Drugs 1999, 8, 1301-1325.
A few compounds having the capacity to selectively inhibit phosphodiesterase 4 are in active development. Examples of these compounds are cipamfylline, arofyline, cilomilast, roflumilast, mesopram and pumafentrine.
We have now found that a novel series of pyridothienopyrimidine derivatives are potent and selective inhibitors of PDE4 and are therefore useful in the treatment or prevention of these pathological conditions, diseases and disorders, in particular asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
The compounds of the present invention can also be used in combination with other drugs known to be effective in the treatment of these diseases. For example, they can be used in combination with steroids or immunosuppressive agents, such as cyclosporin A, rapamycin or T-cell receptor blockers. In this case the administration of the compounds allows a reduction of the dosage of the other drugs, thus preventing the appearance of the undesired side effects associated with both steroids and immunosuppressants.
Like other PDE4 inhibitors (see references above) the compounds of the invention can also be used for blocking the ulcerogenic effects induced by a variety of etiological agents, such as antiinflammatory drugs (steroidal or non-steroidal antiinflammatory agents), stress, ammonia, ethanol and concentrated acids. They can be used alone or in combination with antacids and/or antisecretory drugs in the preventive and/or curative treatment of gastrointestinal pathologies like drug-induced ulcers, peptic ulcers, H. Pylori-related ulcers, esophagitis and gastro-esophageal reflux disease.
They can also be used in the treatment of pathological situations where damage to the cells or tissues is produced through conditions like anoxia or the production of an excess of free radicals. Examples of such beneficial effects are the protection of cardiac tissue after coronary artery occlusion or the prolongation of cell and tissue viability when the compounds of the invention are added to preserving solutions intended for storage of transplant organs or fluids such as blood or sperm. They are also of benefit on tissue repair and wound healing.
Accordingly, the present invention provides the use of the compounds of formula (I) in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4; and methods of treatment of diseases susceptible to amelioration by inhibition of PDE4, which methods comprise the administration of the compounds of formula (I):
wherein
G1 represents a group selected from —CR6R7— or —NR6 being R6 and R7 independently selected from hydrogen atoms and C1-4 alkyl groups;
m and n are integers selected from 0 or 1;
R1 and R2 are independently selected from hydrogen atoms and C1-4 alkyl groups;
R3 represents a group selected from alkyl, amino, monoalkylamino, dialkylamino, aryl, heteroaryl and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being optionally substituted by one or more substituents selected from the group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R8OCO—, alkoxy, R8R9N—CO—, —CN, —CF3, —NR8R9, —SR8 and —SO2NH2 groups wherein R8 and R9 are independently selected from hydrogen atoms and C1-4 alkyl groups;
R4 and R5 are independently selected from the group consisting of hydrogen atoms alkyl groups and groups of formula (II):
wherein p and q are integers selected from 1, 2 and 3; A is either a direct bond or a group selected from —CONR14—, —NR14CO—, —O—, —COO—, —OCO—, —NR14COO—, —OCONR14—, —NR14CONR15—, —S—, —SO—, —SO2—, —COS— and —SCO—; and G2 is a group selected from aryl, heteroaryl or heterocyclyl; wherein the alkyl groups and the group G2 are optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R16OCO—, hydroxy, alkoxy, oxo, R16R17N—CO—, —CN, —CF3, —NR16R17, —SR16 and —SO2NH2 groups; wherein R10 to R17 are independently selected from hydrogen atoms and C1-4 alkyl groups;
and the pharmaceutically acceptable salts and N-oxides thereof;
to a subject in need of treatment.
Among the compounds of formula (I), those wherein R1 is a C1-4 alkyl group are new. Accordingly further objectives of the present invention are the compounds of formula (I) as hereinabove defined wherein R1 and R2 are not simultaneously hydrogen atoms.
Still further objectives of the present invention are to provide processes for preparing said compounds and pharmaceutical compositions comprising an effective amount of said compounds.
As used herein the term alkyl embraces optionally substituted, linear or branched radicals having 1 to 20 carbon atoms or, preferably 1 to 12 carbon atoms. More preferably alkyl radicals are “lower alkyl” radicals having 1 to 8, preferably 1 to 6 and more preferably 1 to 4 carbon atoms.
Examples include methyl, ethyl, n-propyl, i-propyl, n-butyl, sec-butyl, t-butyl, n-pentyl, 1-methylbutyl, 2-methylbutyl, isopentyl, 1-ethylpropyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, n-hexyl, 1-ethylbutyl, 2-ethylbutyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-dimethylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 2-methylpentyl, 3-methylpentyl and iso-hexyl radicals.
When it is mentioned that alkyl radicals may be optionally substituted it is meant to include linear or branched alkyl, alkenyl or alkynyl radicals as defined above, which may be unsubstituted or substituted in any position by one or more substituents, for example by 1, 2 or 3 substituents. When two or more substituents are present, each substituent may be the same or different.
A said optionally substituted alkyl group is typically unsubstituted or substituted with 1, 2 or 3 substituents which may be the same or different. The substituents are preferably selected from halogen atoms, preferably fluorine atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms. Typically, substituents on an alkyl group are themselves unsubstituted. Preferred optionally substituted alkyl groups are unsubstituted or substituted with 1, 2 or 3 fluorine atoms.
As used herein, the term alkoxy (or alkyloxy) embraces optionally substituted, linear or branched oxy-containing radicals each having alkyl portions of 1 to 10 carbon atoms. More preferred alkoxy radicals are “lower alkoxy” radicals having 1 to 8, preferably 1 to 6 and more preferably 1 to 4 carbon atoms.
An alkoxy group is typically unsubstituted or substituted with 1, 2 or 3 substituents which may be the same or different. The substituents are preferably selected from halogen atoms, preferably fluorine atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms. Typically, the substituents on an alkoxy group are themselves unsubstituted.
Preferred alkoxy radicals include methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, sec-butoxy, t-butoxy, trifluoromethoxy, difluoromethoxy, hydroxymethoxy, 2-hydroxyethoxy and 2-hydroxypropoxy.
As used herein, the term monoalkylamino embraces radicals containing an optionally substituted, linear or branched alkyl radicals of 1 to 10 carbon atoms attached to a divalent —NH— radical. More preferred monoalkylamino radicals are “lower monoalkylamino” radicals having 1 to 8, preferably 1 to 6 and more preferably 1 to 4 carbon atoms.
A monoalkylamino group typically contains an alkyl group which is unsubstituted or substituted with 1, 2 or 3 substituents which may be the same or different. The substituents are preferably selected from halogen atoms, preferably fluorine atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms. Typically, the substitutents on a monoalkylamino group are themselves unsubstituted.
Preferred optionally substituted monoalkylamino radicals include methylamino, ethylamino, n-propylamino, i-propylamino, n-butylamino, sec-butylamino, t-butylamino, trifluoromethylamino, difluoromethylamino, hydroxymethylamino, 2-hydroxyethylamino and 2-hydroxypropylamino.
As used herein, the term dialkylamino embraces radicals containing a trivalent nitrogen atoms with two optionally substituted, linear or branched alkyl radicals of 1 to 10 carbon atoms attached thereto. More preferred dialkylamino radicals are “lower dialkylamino” radicals having 1 to 8, preferably 1 to 6 and more preferably 1 to 4 carbon atoms in each alkyl radical.
A dialkylamino group typically contains two alkyl groups, each of which is unsubstituted or substituted with 1, 2 or 3 substituents which may be the same or different. The substituents are preferably selected from halogen atoms, preferably fluorine atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms. Typically, the substituents on a dialkylamino group are themselves unsubstituted.
Preferred optionally substituted dialkylamino radicals include dimethylamino, diethylamino, methyl(ethyl)amino, di(n-propyl)amino, n-propyl(methyl)amino, n-propyl(ethyl)amino, di(i-propyl)amino, i-propyl(methyl)amino, i-propyl(ethyl)amino, di(n-butyl)amino, n-butyl(methyl)amino, n-butyl(ethyl)amino, n-butyl(i-propyl)amino, di(sec-butyl)amino, sec-butyl(methyl)amino, sec-butyl(ethyl)amino, sec-butyl(n-propyl)amino, sec-butyl(i-propyl)amino, di(t-butyl)amino, t-butyl(methyl)amino, t-butyl(ethyl)amino, t-butyl(n-propyl)amino, t-butyl(i-propyl)amino, trifluoromethyl(methyl)amino, trifluoromethyl(ethyl)amino, trifluoromethyl(n-propyl)amino, trifluoromethyl(i-propyl)amino, trifluoromethyl(n-butyl)amino, trifluoromethyl(sec-butyl)amino, difluoromethyl(methyl)amino, difluoromethyl(ethyl)amino, difluoromethyl(n-propyl)amino, difluoromethyl(i-propyl)amino, difluoromethyl(n-butyl))amino, difluoromethyl(sec-butyl)amino, difluoromethyl(t-butyl)amino, difluoromethyl(trifluoromethyl)amino, hydroxymethyl(methyl)amino, ethyl(hydroxymethyl)amino, hydroxymethyl(n-propyl)amino, hydroxymethyl(i-propyl)amino, n-butyl(hydroxymethyl)amino, sec-butyl(hydroxymethyl)amino, t-butyl(hydroxymethyl)amino, difluoromethyl(hydroxymethyl)amino, hydroxymethyl(trifluoromethyl)amino, hydroxyethyl(methyl)amino, ethyl(hydroxyethyl)amino, hydroxyethyl(n-propyl)amino, hydroxyethyl(i-propyl)amino, n-butyl(hydroxyethyl)amino, sec-butyl(hydroxyethyl)amino, t-butyl(hydroxyethyl)amino, difluoromethyl(hydroxyethyl)amino, hydroxyethyl(trifluoromethyl)amino, hydroxypropyl(methyl)amino, ethyl(hydroxypropyl)amino, hydroxypropyl(n-propyl)amino, hydroxypropyl(i-propyl)amino, n-butyl(hydroxypropyl)amino, sec-butyl(hydroxypropyl)amino, t-butyl(hydroxypropyl)amino, difluoromethyl(hydroxypropyl)amino, hydroxypropyl(trifluoromethyl)amino.
As used herein, the term aryl radical embraces typically a C5-C14 monocyclic or polycyclic aryl radical such as phenyl, naphthyl, anthranyl and phenanthryl. Phenyl is preferred.
A said optionally substituted aryl radical is typically unsubstituted or substituted with 1, 2 or 3 substituents which may be the same or different. The substituents are preferably selected from halogen atoms, preferably fluorine atoms, hydroxy groups, alkoxycarbonyl groups in which the alkyl moiety has from 1 to 4 carbon atoms, hydroxycarbonyl groups, carbamoyl groups, nitro groups, cyano groups, C1-C4 alkyl groups, C1-C4 alkoxy groups and C1-C4 hydroxyalkyl groups. When an aryl radical carries 2 or more substituents, the substituents may be the same or different. Unless otherwise specified, the substituents on an aryl group are typically themselves unsubstituted.
As used herein, the term heteroaryl radical embraces typically a 5- to 14-membered ring system, preferably a 5- to 10-membered ring system, comprising at least one heteroaromatic ring and containing at least one heteroatom selected from O, S and N. A heteroaryl radical may be a single ring or two or more fused rings wherein at least one ring contains a heteroatom.
A said optionally substituted heteroaryl radical is typically unsubstituted or substituted with 1, 2 or 3 substituents which may be the same or different. The substituents are preferably selected from halogen atoms, preferably fluorine, chlorine or bromine atoms, alkoxycarbonyl groups in which the alkyl moiety has from 1 to 4 carbon atoms, nitro groups, hydroxy groups, C1-C4 alkyl groups and C1-C4 alkoxy groups. When an heteroaryl radical carries 2 or more substituents, the substituents may be the same or different. Unless otherwise specified, the substituents on a heteroaryl radical are typically themselves unsubstituted.
Examples include pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, furyl, benzofuranyl, oxadiazolyl, oxazolyl, isoxazolyl, benzoxazolyl, imidazolyl, benzimidazolyl, thiazolyl, thiadiazolyl, thienyl, pyrrolyl, pyridinyl, benzothiazolyl, indolyl, indazolyl, purinyl, quinolyl, isoquinolyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, quinolizinyl, cinnolinyl, triazolyl, indolizinyl, indolinyl, isoindolinyl, isoindolyl, imidazolidinyl, pteridinyl, thianthrenyl, pyrazolyl, 2H-pyrazolo[3,4-d]pyrimidinyl, 1H-pyrazolo[3,4-d]pyrimidinyl, thieno[2,3-d]pyrimidnyl and the various pyrrolopyridyl radicals.
Oxadiazolyl, oxazolyl, pyridyl, pyrrolyl, imidazolyl, thiazolyl, thiadiazolyl, thienyl, furanyl, quinolinyl, isoquinolinyl, indolyl, benzoxazolyl, naphthyridinyl, benzofuranyl, pyrazinyl, pyrimidinyl and the various pyrrolopyridyl radicals are preferred.
As used herein, the term heterocyclyl radical embraces typically a non-aromatic, saturated or unsaturated C3-C10 carbocyclic ring, such as a 5, 6 or 7 membered radical, in which one or more, for example 1, 2, 3 or 4 of the carbon atoms preferably 1 or 2 of the carbon atoms are replaced by a heteroatom selected from N, O and S. Saturated heterocyclyl radicals are preferred. A heterocyclic radical may be a single ring or two or more fused rings wherein at least one ring contains a heteroatom. When a heterocyclyl radical carries 2 or more substituents, the substituents may be the same or different. A N-containing heterocyclyl radical is an heterocyclyl radical in which at least one carbon atom of the carbocyclyl ring is replaced by a nitrogen atom.
A said optionally substituted heterocyclyl radical is typically unsubstituted or substituted with 1, 2 or 3 substituents which may be the same or different. The substituents are preferably selected from halogen atoms, preferably fluorine atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms. Typically, the substituents on a heterocyclyl radical are themselves unsubstituted.
Examples of heterocyclic radicals include piperidyl, pyrrolidyl, pyrrolinyl, piperazinyl, morpholinyl, thiomorpholinyl, pyrrolyl, pyrazolinyl, pirazolidinyl, quinuclidinyl, triazolyl, pyrazolyl, tetrazolyl, cromanyl, isocromanyl, imidazolidinyl, imidazolyl, oxiranyl, azaridinyl, 4,5-dihydro-oxazolyl and 3-aza-tetrahydrofuranyl. Preferred heterocyclyl radicals are selected from piperidyl, pyrrolidyl, piperazinyl, morpholinyl and thiomorpholinyl.
Where a heterocyclyl radical carries 2 or more substituents, the substituents may be the same or different.
As used herein, some of the atoms, radicals, moieties, chains and cycles present in the general structures of the invention are “optionally substituted”. This means that these atoms, radicals, moieties, chains and cycles can be either unsubstituted or substituted in any position by one or more, for example 1, 2, 3 or 4, substituents, whereby the hydrogen atoms bound to the unsubstituted atoms, radicals, moieties, chains and cycles are replaced by chemically acceptable atoms, radicals, moieties, chains and cycles. When two or more substituents are present, each substituent may be the same or different. The substituents are typically themselves unsubstituted.
As used herein, the term halogen atom embraces chlorine, fluorine, bromine and iodine atoms. A halogen atom is typically a fluorine, chlorine or bromine atom, most preferably chlorine or fluorine. The term halo when used as a prefix has the same meaning.
Compounds containing one or more chiral centre may be used in enantiomerically or diastereoisomerically pure form, or in the form of a mixture of isomers.
As used herein, the term pharmaceutically acceptable salt embraces salts with a pharmaceutically acceptable acid or base. Pharmaceutically acceptable acids include both inorganic acids, for example hydrochloric, sulphuric, phosphoric, diphosphoric, hydrobromic, hydroiodic and nitric acid and organic acids, for example citric, fumaric, maleic, malic, mandelic, ascorbic, oxalic, succinic, tartaric, benzoic, acetic, methanesulphonic, ethanesulphonic, benzenesulphonic or p-toluenesulphonic acid. Pharmaceutically acceptable bases include alkali metal (e.g. sodium or potassium) and alkali earth metal (e.g. calcium or magnesium) hydroxides and organic bases, for example alkyl amines, arylalkyl amines and heterocyclic amines.
As used herein, an N-oxide is formed from the tertiary basic amines or imines present in the molecule, using a convenient oxidising agent.
It is one embodiment of the present invention the use of the compounds of formula (I)
wherein:
G1 represents a group selected from —CR6R7— or —NR6 being R6 and R7 independently selected from hydrogen atoms and C1-4 alkyl groups;
m and n are integers selected from 0 or 1;
R1 and R2 are independently selected from hydrogen atoms and C1-4 alkyl groups;
R3 represents a group selected from alkyl, amino, monoalkylamino, dialkylamino, aryl, heteroaryl and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being optionally substituted by one or more substituents selected from the group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R8OCO—, alkoxy, R8R9N—CO—, —CN, —CF3, —NR8R9, —SR8 and —SO2NH2 groups wherein R8 and R9 are independently selected from hydrogen atoms and C1-4 alkyl groups;
R4 and R5 are independently selected from the group consisting of hydrogen atoms alkyl groups and groups of formula (II):
wherein p and q are integers selected from 1, 2 and 3; A is either a direct bond or a group selected from —CONR14—, —NR14CO—, —O—, —COO—, —OCO—, —NR14COO—, —OCONR14—, —NR14CONR15—, —S—, —SO—, —SO2—, —COS— and —SCO—; and G2 is a group selected from aryl, heteroaryl or heterocyclyl; wherein the alkyl groups and the group G2 are optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R16OCO—, alkoxy, R16R17N—CO—, —CN, —CF3, —NR16R17, —SR16 and —SO2NH2 groups; wherein R10 to R17 are independently selected from hydrogen atoms and C1-4 alkyl groups;
and the pharmaceutically acceptable salts and N-oxides thereof; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4.
It is another embodiment of the present invention a method of treatment of diseases susceptible to amelioration by inhibition of PDE4, which methods comprise the administration of the compounds of formula (I):
wherein
G1 represents a group selected from —CR6R7— or —NR6 being R6 and R7 independently selected from hydrogen atoms and C1-4 alkyl groups;
m and n are integers selected from 0 or 1;
R1 and R2 are independently selected from hydrogen atoms and C1-4 alkyl groups;
R3 represents a group selected from alkyl, amino, monoalkylamino, dialkylamino, aryl, heteroaryl and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being optionally substituted by one or more substituents selected from the group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R8OCO—, alkoxy, R8R9N—CO—, —CN, —CF3, —NR8R9, —SR8 and —SO2NH2 groups wherein R8 and R9 are independently selected from hydrogen atoms and C1-4 alkyl groups;
R4 and R5 are independently selected from the group consisting of hydrogen atoms alkyl groups and groups of formula (II):
wherein p and q are integers selected from 1, 2 and 3; A is either a direct bond or a group selected from —CONR14—, —NR14CO—, —O—, —COO—, —OCO—, —NR14COO—, —OCONR14—, —NR4CONR15—, —S—, —SO—, —SO2—, —COS— and —SCO—; and G2 is a group selected from aryl, heteroaryl or heterocyclyl; wherein the alkyl groups and the group G2 are optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R16OCO—, alkoxy, R16R17N—CO—, —CN, —CF3, —NR16R17—SR16 and —SO2NH2 groups; wherein R10 to R17 are independently selected from hydrogen atoms and C1-4 alkyl groups;
and the pharmaceutically acceptable salts and N-oxides thereof;
to a subject in need of treatment.
It is one embodiment of the present invention the use of the compounds of formula (I) wherein G1 is a group —CR6R7— wherein R6 and R7 are independently selected from hydrogen atoms and C1-4 alkyl groups, preferably a group —CH2— in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is another embodiment of the present invention the use of the compounds of formula (I) wherein R1 and R2 are both methyl groups in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is still another embodiment of the present invention the use of the compounds of formula (I) wherein m and n have both the value of 1; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is also another embodiment of the present invention the use of the compounds of formula (I) wherein R3 is selected from monoalkylamino, dialkylamino and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being optionally substituted by one or more substituents selected from the group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R8OCO—, alkoxy, R8R9NCO—, —CN, —CF3, —NR8R9, —SR8 and —SO2NH2 groups wherein R8 and R9 are independently selected from hydrogen atoms and C1-4 alkyl groups; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is still another embodiment of the present invention the use of the compounds of formula (I) wherein R3 is selected from monoalkylamino, dialkylamino and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being non substituted; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is still another embodiment of the present invention the use of the compounds of formula (I) wherein R4 is a hydrogen atom; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is another embodiment of the present invention the use of a compound of formula (I) wherein R5 is a group of formula (III)
wherein q is an integer selected from 1 or 2, A represents a direct bond or a group —CONH— and G2 is a group selected from aryl, heteroaryl or heterocyclyl; wherein the group G2 is optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R16OCO—, alkoxy, R16R17NCO—, —CN, —CF3, —NR16R17, —SR16 and —SO2NH2 groups; wherein R16 and R17 are independently selected from hydrogen atoms and C1-4 alkyl groups; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is another embodiment of the present invention to use compounds of formula (I) wherein the group G2 are optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R16OCO—, hydroxy, alkoxy, oxo, R16R17N—CO—, —CN, —CF3, —NR16R17, —SR16 and —SO2NH2 groups; wherein R16 and R17 are independently selected from hydrogen atoms and C1-4 alkyl groups.
It is yet another embodiment of the present invention to use the compounds of formula (I) wherein R5 is a group of formula (III)
q is an integer selected from 1 or 2, A represents a direct bond or a group —CONH— and G2 is optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkoxy and R16OCO— groups; wherein R16 is selected from hydrogen atoms and C1-4 alkyl groups; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is a particularly preferred embodiment of the present invention to use the compounds of formula (I) wherein G1 is a —CH2— group, R1 and R2 are both methyl groups, m and n have both the value of 1, R3 is selected from monoalkylamino, dialkylamino and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being non substituted, R4 is a hydrogen atom and R5 is a group of formula (III)
wherein q is an integer selected from 1 or 2, A represents a direct bond or a group —CONH— and G2 is a group selected from aryl, heteroaryl or heterocyclyl; wherein the group G2 is optionally substituted by one or more substituents selected from group consisting of halogen atoms and lower alkoxy, oxo and R16OCO— groups; wherein R16 is selected from hydrogen atoms and C1-4 alkyl groups; in the manufacture of a medicament for the treatment of diseases susceptible of being improved by inhibition of PDE4, in particular for the treatment or prevention of a disorder which is asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable bowel disease.
It is another embodiment of the present invention the compounds of formula (I) wherein R1 is selected from C1-4 alkyl groups and the m, n, G1, R2, R3, R4 and R5 are as hereinabove defined.
According to another embodiment of the present invention in the compounds of formula (I) R1 is selected from C1-4 alkyl groups and G1 is a group —CR6R7— wherein R6 and R7 are independently selected from hydrogen atoms and C1-4 alkyl groups, preferably a group —CH2—.
According to another embodiment of the present invention in the compounds of formula (I) R1 and R2 are both methyl groups.
According to still another embodiment of the present invention in the compounds of formula (I) R1 is selected from C1-4 alkyl groups and m and n have both the value of 1.
According to still another embodiment of the present invention in the compounds of formula (I) R1 is selected from C1-4 alkyl groups and R3 is selected from monoalkylamino, dialkylamino and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being optionally substituted by one or more substituents selected from the group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R8OCO—, alkoxy, R8R9N—CO—, —CN, —CF3, —NR8R9, —SR8 and —SO2NH2 groups wherein R8 and R9 are independently selected from hydrogen atoms and C1-4 alkyl groups. More preferably R3 is selected from monoalkylamino, dialkylamino and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being non substituted.
According to still another embodiment of the present invention in the compounds of formula (I) R1 is selected from C1-4 alkyl groups and R4 is a hydrogen atom.
According to still another embodiment of the present invention in the compounds of formula (I) R1 is selected from C1-4 alkyl groups and R5 is a group of formula (III)
wherein q is an integer selected from 1 or 2, A represents a direct bond or a group —CONH— and G2 is a group selected from aryl, heteroaryl or heterocyclyl; wherein the group G2 is optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkyl, alkoxyalkyl, arylalkyl, R16OCO—, alkoxy, R16R17NCO—, —CN, —CF3, —NR16R17—SR16 and —SO2NH2 groups; wherein R16 and R17 are independently selected from hydrogen atoms and C1-4 alkyl groups.
According to another embodiment of the present invention in the compounds of formula (I) R1 is selected from C1-4 alkyl groups and R5 is a group of formula (III)
wherein q is an integer selected from 1 or 2, A represents a direct bond or a group —CONH— and G2 is optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkoxy and R16OCO— groups; wherein R16 is selected from hydrogen atoms and C1-4 alkyl groups.
In a particularly preferred embodiment of the present invention in the compounds of formula (I) R1 is selected from C1-4 alkyl groups, G1 is a —CH2— group, R1 and R2 are both methyl groups, m and n have both the value of 1, R3 is selected from monoalkylamino, dialkylamino and saturated N-containing heterocyclyl groups bound through the nitrogen atom to the piridine ring, all of them being non substituted, R4 is a hydrogen atom and R5 is a group of formula (III)
wherein q is an integer selected from 1 or 2, A represents a direct bond or a group —CONH— and G2 is a group selected from aryl, heteroaryl or heterocyclyl; wherein the group G2 is optionally substituted by one or more substituents selected from group consisting of halogen atoms and alkoxy and R16OCO— groups; wherein R16 is selected from hydrogen atoms and C1-4 alkyl groups.
Particular individual compounds of the invention include:
Of outstanding interest are:
According to a further feature of the present invention, the compounds of formula (I) may be prepared by one of the processes described below.
Compounds Ia wherein R3 is a monosubstituted, disubstituted or unsubstituted amino group may be obtained as shown in Scheme 1.
A ketone of formula VI, wherein G1, m, n, R1 and R2 are as hereinbefore defined, is condensed with malononitrile in the presence of carbon disulfide to yield the heterocycle of formula II, according to the method described by E. G. Paronikyan and A. S. Noravyan at Chem. Heterocycl. Compd (NY), 1999, 35(7), 799-803. Ketones VI are commercially available or prepared according to the methods described at C. Ainsworth Org. Synth., 1959, 39, 536, J. Cologne, A. Varagnat Bull. Soc. Chim. France, 1964, 10, 2499-504, and E. M. Kosower, T. S. Sorensen, 1963, 28, 687.
Reaction of compound II with an amine HNR′R″ of formula XIV, wherein R′ and R″ are as hereinbefore defined, yields the pyridine derivative III, as described by K. Gewald et al at J. Prakt. Chem., 1973, 315(4), 679-689.
Subsequent cyclocondensation of compound III with 2-chloroacetamide in the presence of a base such as potasium carbonate affords the thienopyridine compound IV, according to C. Peinador et al J. Het. Chem., 1992, 29, 1693 or C. Peinador et al Bioorg. Med. Chem., 1998, 6, 1911.
The pyridothienopyrimidine derivative V is sinthesized by cyclisation of intermediate IV with a orthoformate derivative HC(OR6)3, wherein R6 is a C1-4 alkyl group, as described at C. Peinador et al Bioorg. Med. Chem., 1998, 6, 1911, or formic acid or a reactive derivative thereof. The reactive derivative of the formic acid is preferably the acid halide, orthoester or anhydride. The reaction can be carried out in a solvent, preferably a polar aprotic solvent, such as N,N-dimethylformamide, dioxane, acetone or tetrahydrofuran, in the presence of an organic base, preferably an amine base, such as triethylamine and at a temperature from 15° C. to 40° C. The reaction can also be carried out in the absence of a solvent, in which case an excess of formic acid or reactive derivative of formic acid is used and the mixture is heated at a temperature from 40° C. to its boiling point.
The corresponding chloroimine derivative of V is sinthesized using phosphorous oxychloride as solvent, and the resulting intermediate is reacted with an amine of formula XV, wherein R4 and R5 are as hereinbefore defined, to give the desired final compound Ia.
When the defined groups R′, R″ and R1 to R6 are susceptible to chemical reaction under the conditions of the hereinbefore described processes or are incompatible with said processes, conventional protecting groups may be used in accordance with standard practice, for example see T. W. Greene and P. G. M. Wuts in ‘Protective Groups in Organic Chemistry’, 3rd Edition, John Wiley & Sons (1999). It may be that deprotection will form the last step in the synthesis of compounds of formula I.
Another route for the obtention of compounds Ib is shown in Scheme 2.
Ketone VI, wherein G1, m, n, R1 and R2 are as hereinbefore defined, reacts with dimethyl carbonate in the presence of a strong base such as sodium hydride in tetrahydrofurane to yield the diketone VII, according to the method described by L. A. Paquette at J. Org. Chem., 1991, 56, 6199. Ketones VI are commercially available or prepared according to the methods described at C. Ainsworth Org. Synth., 1959, 39, 536, J. Cologne, A. Varagnat Bull. Soc. Chim. France, 1964, 10, 2499-504, and E. M. Kosower, T. S. Sorensen, 1963, 28, 687.
Reaction of compound VII with cyanoacetamide in methanol under refluxing conditions with the presence of potassium hydroxide yields the pyridine derivative VIII, as described by E. Wenkert et al. at J. Am. Chem. Soc., 1965, 87, 5461. The same reference applies for the conversion of VII to the 1,6-dichloropyridine derivative IX by reaction with phosphorous oxychloride without solvent at 150-170° C. in a sealed tube.
IX is converted to X under classical Suzuki coupling conditions by reaction with a boronic acid of a lower alkyl boronate of formula XVI in the presence of potassium carbonate and tetrakis(triphenylphosphine)palladium(0) under reflux of dioxane, where the boronic acids R3B(OH)2 or their corresponding boronates are commercially available or sinthesized by common methodology, being R3 as hereinbefore defined.
Subsequent cyclocondensation of compound X with 2-mercaptoacetamide in the presence of a base such as potasium carbonate affords the thienopyridine compound XI, according to Santilli, A. A.; Kim, D. H.; Wanser, S. V.; J Heterocycl Chem, 1971, 8, 445 or Schneller, S. W.; Clough, F. W.; J Heterocycl Chem, 1975,12, 513.
The pyridothienopyrimidine derivative XII is sinthesized by cyclisation of intermediate XI with a orthoformate derivative HC(OR6)3, wherein R5 is a C1-4 alkyl group, as described at C. Peinador et al Bioorg. Med. Chem., 1998, 6, 1911, or formic acid or a reactive derivative thereof. The reactive derivative of formic acid is preferably the acid halide, orthoester or anhydride. The reaction can be carried out in a solvent, preferably a polar aprotic solvent, such as N,N-dimethylformamide, dioxane, acetone or tetrahydrofuran, in the presence of an organic base, preferably an amine base, such as triethylamine and at a temperature from 15° C. to 40° C. The reaction can also be carried out in the absence of a solvent, in which case an excess of formic acid or reactive derivative of the formic acid is used and the mixture is heated at a temperature from 40° C. to its boiling point.
The corresponding chloroimine derivative XIII is sinthesized using phosphorous oxychloride as solvent, and the resulting intermediate is reacted with an amine of formula XV, wherein R4 and R5 are as hereinbefore defined, to give the desired final compound Ib.
The pharmaceutically acceptable salts of the compounds of the present invention represented by formula Ia and Ib may be acid addition salts or alkali addition salts. Examples of the acid addition salts include those formed with a mineral acid such as, for example, hydrochloric, hydrobromic, hydroiodic, sulfaric, nitric, phosphoric, or with an organic acid such as, for example, acetic, maleic, fumaric, citric, oxalic, succinic, tartaric, malic, mandelic, methanesulfonic, and p-toluenesulfonic. Examples of the alkali addition salts include inorganic salts such as, for example sodium, potassium, calcium and ammonium salts and organic alkali salts such as, for example, ethylenediamine, ethanolamine, N,N-dialkylenethanolamine, triethanolamine and basic amino acid salts
The compounds of the present invention represented by the above described formula (Ia and Ib) may include enantiomers depending on their asymmetry or diastereoisomers. The single isomers and mixtures of the isomers fall within the scope of the present invention.
The compounds of formulae VI, XIV, XV and XVI are known compounds or can be prepared by analogy with known methods.
Compounds to be tested were resuspended in DMSO at a stock concentration of 1 mM. The compounds were tested at different concentrations varying from 10 μM to 10 pM to calculate an IC50. These dilutions were done in 96-well plates. In some cases, plates containing diluted compounds were frozen before being assayed. In these cases, the plates were thawed at room temperature and stirred for 15 minutes.
Ten microliters of the diluted compounds were poured into a “low binding” assay plate. Eighty microliters of reaction mixture containing 50 mM Tris pH 7.5, 8.3 mM MgCl2, 1.7 mM EGTA, and 15 nM [3H]-cAMP were added to each well. The reaction was initiated by adding ten microliters of a solution containing PDE4. The plate was then incubated under stirring for 1 hour at room temperature. After incubation the reaction was stopped with 50 microlitres of SPA beads, and the reaction was allowed to incubate for another 20 minutes at room temperature before measuring radioactivity using standard instrumentation.
The reaction mixture was prepared by adding 90 ml of H2O to 10 ml of 10× assay buffer (500 mM Tris pH 7.5, 83 mM MgCl2, 17 mM EGTA), and 40 microlitres 1 μCi/μL [3H]-cAMP. SPA beads solution was prepared by adding 500 mg to 28 ml H2O for a final concentration of 20 mg/ml beads and 18 mM zinc sulphate.
The results are shown in Table 1.
It can be seen from Table 1 that the compounds of formula (I) are potent inhibitors of phosphodiesterase 4 (PDE 4). Preferred pyridothienopyrimidine derivatives of the invention possess an IC50 value for the inhibition of PDE4 (determined as defined above) of less than 100 nM, preferably less than 50 nM and most preferably less than 30 nM.
The compounds are also capable of blocking the production of some pro-inflammatory cytokines such as, for example, TNFα. Thus, they can be used in the treatment of allergic, inflammatory and immunological diseases, as well as those diseases or conditions where the blockade of pro-inflammatory cytokines or the selective inhibition of PDE 4 could be of benefit.
These disease states include asthma, chronic obstructive pulmonary disease, allergic rhinitis, rheumatoid arthritis, osteoarthritis, osteoporosis, bone-formation disorders, glomerulonephritis, multiple sclerosis, ankylosing spondylitis, Graves ophtalmopathy, myasthenia gravis, diabetes insipidus, graft rejection, gastrointestinal disorders such as ulcerative colitis or Crohn disease, septic shock, adult distress respiratory syndrome, and skin diseases such as atopic dermatitis, contact dermatitis, acute dermatomyositis and psoriasis. They can also be used as improvers of cerebrovascular function as well as in the treatment of other CNS related diseases such as dementia, Alzheimer's disease, depression, and as nootropic agents.
The compounds of the present invention are also of benefit when administered in combination with other drugs such as steroids and immunosuppressive agents, such as cyclosporin A, rapamycin or T-cell receptor blockers. In this case the administration of the compounds allows a reduction of the dosage of the other drugs, thus preventing the appearance of the undesired side effects associated with both steroids and immunosuppressants. The compounds of the invention have also shown their efficacy in blocking, after preventive and/or curative treatment, the erosive and ulcerogenic effects induced by a variety of etiological agents, such as antiinflammatory drugs (steroidal or non-steroidal antiinflammatory agents), stress, ammonia, ethanol and concentrated acids.
They can be used alone or in combination with antacids and/or antisecretory drugs in the preventive and/or curative treatment of gastrointestinal pathologies like drug-induced ulcers, peptic ulcers, H. Pylori-related ulcers, esophagitis and gastro-esophageal reflux disease. They can also be used in the treatment of pathological situations where damage to the cells or tissues is produced through conditions like anoxia or the production of an excess of free radicals. Examples of such beneficial effects are the protection of cardiac tissue after coronary artery occlusion or the prolongation of cell and tissue viability when the compounds of the invention are added to preserving solutions intended for storage of transplant organs or fluids such as blood or sperm. They are also of benefit on tissue repair and wound healing.
Accordingly, the pyridothienopyrimidine derivatives of the invention and pharmaceutically acceptable salts thereof, and pharmaceutical compositions comprising such compound and/or salts thereof, may be used in a method of treatment of disorders of the human body which comprises administering to a patient requiring such treatment an effective amount of a pyridothienopyrimidine derivative of the invention or a pharmaceutically acceptable salt thereof.
The present invention also provides pharmaceutical compositions which comprise, as an active ingredient, at least a pyridothienopyrimidine derivative of formula (I) or a pharmaceutically acceptable salt thereof in association with a pharmaceutically acceptable excipient such as a carrier or diluent. The active ingredient may comprise 0.001% to 99% by weight, preferably 0.01% to 90% by weight, of the composition depending upon the nature of the formulation and whether further dilution is to be made prior to application. Preferably the compositions are made up in a form suitable for oral, topical, nasal, rectal, percutaneous or injectable administration.
The pharmaceutically acceptable excipients which are admixed with the active compound, or salts of such compound, to form the compositions of this invention are well-known per se and the actual excipients used depend inter alia on the intended method of administering the compositions.
Compositions for oral administration may take the form of tablets, retard tablets, sublingual tablets, capsules, inhalation aerosols, inhalation solutions, dry powder inhalation, or liquid preparations, such as mixtures, elixirs, syrups or suspensions, all containing the compound of the invention; such preparations may be made by methods well-known in the art.
The diluents which may be used in the preparation of the compositions include those liquid and solid diluents which are compatible with the active ingredient, together with colouring or flavouring agents, if desired. Tablets or capsules may conveniently contain between 2 and 500 mg of active ingredient or the equivalent amount of a salt thereof.
The liquid composition adapted for oral use may be in the form of solutions or suspensions. The solutions may be aqueous solutions of a soluble salt or other derivative of the active compound in association with, for example, sucrose to form a syrup. The suspensions may comprise an insoluble active compound of the invention or a pharmaceutically acceptable salt thereof in association with water, together with a suspending agent or flavouring agent.
Compositions for parenteral injection may be prepared from soluble salts, which may or may not be freeze-dried and which may be dissolved in pyrogen free aqueous media or other appropriate parenteral injection fluid.
Compositions for topical administration may take the form of ointments, creams or lotions, all containing the compound of the invention; such preparations may be made by methods well-known in the art.
Effective doses are normally in the range of 10-600 mg of active ingredient per day. Daily dosage may be administered in one or more treatments, preferably from 1 to 4 treatments, per day.
The syntheses of the compounds of the invention and of the intermediates for use therein are illustrated by the following Examples (including Preparation Examples (Preparations 1 to 63)) which do not limit the scope of the invention in any way.
1H Nuclear Magnetic Resonance Spectra were recorded on a Varian Gemini 300 spectrometer.
Low Resolution Mass Spectra (m/z) were recorded on a Micromass ZMD mass spectrometer using ESI ionization.
Melting points were recorded using a Perkin Elmer DSC-7 apparatus.
The chromatographic separations were obtained using a Waters 2690 system equipped with a Symmetry C18 (2.1×10 mm, 3.5 mM) column. The mobile phase was formic acid (0.4 mL), ammonia (0.1 mL), methanol (500 mL) and acetonitrile (500 mL) (B) and formic acid (0.46 mL), ammonia (0.115 mL) and water (1000 mL) (A): initially from 0% to 95% of B in 20 min, and then 4 min. with 95% of B. The reequilibration time between two injections was 5 min. The flow rate was 0.4 mL/min. The injection volume was 5 microliter. Diode array chromatograms were collected at 210 nM.
To a suspension of copper(I) cyanide (2.46 g, 27.5 mmol) cooled at 0° C. a 3.0M solution of methyl magnesium bromide (18.25 ml, 54.8 mmol) is dropwise added. Once the addition is completed, the reaction mixture is stirred for 30 min more at 0° C. and then cooled to −78° C. A solution of 3-methyl-2-cyclohexen-1-one (1.0 g, 9.07 mmol) in ethyl ether (15 ml) is then dropwise added. When the addition is over, the reaction mixture is stirred between 40° C. and −20° C. for two hours. Finally, an aqueous solution of phosphate buffer (pH=7.2, 90 ml) is carefully added to quench the reaction, followed by saturated solution of ammonium chloride (35 ml). The system is allowed to reach room temperature and the two phases separated. The aqueous phase is extracted twice with ethyl ether and the organic phases washed with brine, dried over magnesium sulphate, filtered and the solvents evaporated under vacuum. 1.08 g of the desired final compound, as an orange oil, is obtained, pure enough so as to be used in the next synthetic step without further purification. Yield=94%
1H NMR (200 MHz, CDCl3) δ ppm 0.98 (s, 6H) 1.59 (m, 2H) 1.89 (m, 2H) 2.16 (s, 2H) 2.28 (t, J=6.62 Hz, 2H)
2,2-Dimethylcyclohexanone (1.15 g, 9.07 mmol, see Preparation 1) is solved in methanol (1.10 ml) and carbon disulfide (1.10 ml, 18.2 mmol) is added in one portion. Malononitrile (0.60 g, 9.07 mmol) is added portionwise and, finally, triethylamine is added (0.44 ml). The reaction mixture is stirred at room temperature for 48 h. The solvent is evaporated under vacuum and 0.84 g of 2-(3,3-dimethylcyclohexylidene)malononitrile were isolated by flash chromatography, eluting first with CH2Cl2 and next with the mixture of solvents. This intermediate compound was solved in methanol (0.56 ml) and carbon disulfide (2 equivalents) and triethylamine (0.35 eq.) were added. After 48 h stirring at room temperature, a solid is filtered and washed with methanol. It weighs 0.45 g and its 1HNMR is consistent with the final product. From the methanolic phase, another 0.5 g of the final compound were isolated by flash chromatography, eluting with CH2Cl2: MeOH 95:5. Global yield=42%.
1H NMR (200 MHz, CDCl3) δ ppm 1.01 (s, 6H) 1.57 (m, 2H) 2.52 (s, 2H) 2.76 (t, J=6.62 Hz, 2H) 5.67 (s, 2H)
The product resulting from preparation 2 (0.94 g, 3.75 mmol) is suspended in ethanol (4.5 ml) and morpholine (1.86 ml, 21.4 mmol) is added. The reaction mixture is refluxed under nitrogen overnight. Then the system is allowed to reach room temperature and the reaction mixture is left in an ice bath for two hours. The solid formed is filtrated and washed twice with ethanol. After drying, 0.35 g of the final compound are obtained as a dark solid, pure enough to perform the next step. Yield=31%.
1H NMR (200 MHz, CDCl3) δ ppm 1.01 (s, 6H) 1.5 (t, J=6.99 Hz, 2H) 2.2 (m, 1H) 2.47 (t, J=6.99, 2H) 2.6 (s, 2H) 3.3 (m, 4H) 3.9 (m, 4H)
To a suspension of 3-mercapto-6,6-dimethyl-1-morpholin-4-yl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (0.35 g, 1.15 mmol, see Preparation 3) in ethanol (15 ml), potasium carbonate (0.38 g, 2.77 mmol) and 2-chloroacetamide (0.12 g, 1.27 mmol) are added, and the reaction mixture is then refluxed for 3 h. The solvent is evaporated under vacuum and water is added to the residue: the precipitated solid is filtered and dried. It weighs 0.31 g and its 1H-RMN is consistent with the desired product. Yield=74%. 1H NMR (200 MHz, CDCl3) δ ppm 1.6 (s, 6H) 2.7 (t, J=6.99 Hz, 2H) 3.0 (s, 2H) 3.2 (m, 4H) 3.4 (m, 2H) 3.85 (m, 6H) 5.25 (s, 2H) 6.25 (s, 2H)
1-Amino-8,8-dimethyl-5-morpholin-4-yl-8,9-dihydro-6H-pyrano[4,3-d]thieno[2,3-b]pyridine-2-carboxamide (0.31 g, 0.85 mmol, see Preparation 4) is supended in ethyl orthoformate (6 ml) and p-toluensulfonic acid hydrate (0.02 g, 0.1 mmol) is added. This mixture is heated under reflux overnight. Then the reaction mixture is allowed to reach room temperature and left in an ice bath for two hours. The precipitated formed is filtered and washed with ethyl ether. After drying it weighs 0.15 g and its 1H-RMN is consistent with the desired compound. Yield=47%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 1.8 (m, 4H) 3.3 (m, 4H) 3.35 (s, 1H) 3.85 (m, 4H) 8.1 (s, 1H)
The final product of preparation 5 (0.15 g, 0.40 mmol) is suspended in phosphorous oxychloride (2 ml) and heated to reflux for 90 min. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between chloroform and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with chloroform and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.16 g of a brownish solid is obtained, whose 1HNMR is consistent with the desired final product. Quantitative yield.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.8 (t, J=6.99 Hz, 2H) 3.4 (m, 6H) 3.9 (m, 4H) 9.05 (s, 1H)
The product resulting from preparation 2 (3.0 g, 11.98 mmol) is suspended in ethanol (15 ml) and thiomorpholine (6.87 ml, 68.3 mmol) is added. The reaction mixture is refluxed under nitrogen overnight. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting first with dichloromethane and then with the mixture CH2Cl2:MeOH 9:1. 3.69 g of a mixture 55:45 of the final compound and starting material, respectively, are obtained. The next synthetic step is performed with this mixture. 1H NMR (200 MHz, CDCl3) 8 ppm 1.01 (s, 6H) 1.5 (t, J=6.99 Hz, 2H) 2.2 (m, 1H) 2.47 (t, J=6.99, 2H) 2.6 (s, 2H) 3.3 (m, 4H) 3.9 (m, 4H)
To a suspension of 3-mercapto-6,6-dimethyl-1-thiomorpholin-4-yl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (3.69 g, mixture of products, see Preparation 7) in ethanol (175 ml), potasium carbonate (3.83 g, 27.7 mmol) and 2-chloroacetamide (1.19 g, 12.7 mmol) are added, and the reaction mixture is then refluxed for 18 h. The solvent is evaporated under vacuum and water is added to the residue: the precipitated solid is filtered and dried. It is then purified by flash chromatography, eluting with CH2Cl2:MeOH 95:5. 0.85 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield (calculated from the starting material of Preparation 35)=20%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.5 (m, 2H) 2.7 (t, J=6.99 Hz, 2H) 2.8 (m, 4H) 3.0 (s, 2H) 3.4 (m, 4H) 5.25 (s, 2H) 6.45 (s, 2H)
1-Amino-8,8-dimethyl-5-thiomorpholin-4-yl-8,9-dihydro-6H-pyrano[4,3-d]thieno[2,3-b]pyridine-2-carboxamide (0.85 g, 2.26 mmol, see Preparation 8) is supended in ethyl orthoformate (17 ml) and p-toluensulfonic acid hydrate (0.05 g, 0.23 mmol) is added. This mixture is heated under reflux for 3 h. Then the reaction mixture is allowed to reach room temperature and left in an ice bath for two hours. The precipitated formed is filtered and washed with ethyl ether. After drying it weighs 0.46 g and its 1H-RMN is consistent with the desired compound. Yield=53%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.7 (m, 2H) 2.9 (m, 4H) 3.35 (s, 2H) 3.55 (m, 4H) 8.25 (s, 1H)
The final product of preparation 9 (0.46 g, 1.18 mmol) is suspended in phosphorous oxychloride (10 ml) and heated to reflux for 90 min. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between chloroform and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with chloroform and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.48 g of a solid is obtained, whose 1HNMR is consistent with the desired final product. Quantitative yield.
1H NMR (200 MHz, CDCl3) δ ppm 1.15 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.75 (t, J=6.99 Hz, 2H) 2.85 (m, 4H) 3.4 (s, 2H) 3.6 (m, 4H) 9.05 (s, 1H)
The product resulting from preparation 2 (1.0 g, 3.99 mmol) is suspended in ethanol (7 ml) and N-methylpiperazine (2.53 ml, 22.7 mmol) is added. The reaction mixture is refluxed under nitrogen overnight. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting first with dichloromethane and then with the mixture CH2Cl2:MeOH 95:5. 0.9 g of the final compound are obtained.
Yield=71%.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.10 (s, 6H) 1.50 (m, 2H) 2.5 (m, 2H) 2.60 (s, 2H) 2.65 (s, 3H) 2.80 (bs, 1H) 2.95 (m, 4H) 3.55 (m, 4H)
To a suspension of 3-mercapto-6,6-dimethyl-1-(4-methylpiperazin-1-yl)-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (0.9 g, see Preparation 11) in ethanol (50 ml), potasium carbonate (0.83 g, 5.97 mmol) and 2-chloroacetamide (0.29 g, 3.12 mmol) are added, and the reaction mixture is then refluxed for 6 h and then left overnight at room temperature. The solvent is evaporated under vacuum and water is added to the residue: the precipitated solid is filtered and dried. 0.95 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=90%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.5 (t, J=6.99 Hz, 2H) 2.40 (s, 3H) 2.65 (m, 6H) 3.0 (s, 2H) 3.30 (m, 4H) 5.25 (s, 2H) 6.45 (s, 2H)
1-Amino-8,8-dimethyl-5-(4-methylpiperazine-1-yl)-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamide (0.95 g, 2.55 mmol, see Preparation 12) is supended in ethyl orthoformate (20 ml) and p-toluensulfonic acid hydrate (0.05 g, 0.26 mmol) is added. This mixture is heated under reflux for 4 h. Then the reaction mixture is allowed to reach room temperature and left in an ice bath for two hours, but no solid precipitates. The excess of ethyl ortoformate is evaporated under vacuum and the residue is solved in chloroform. This organic phase is washed with water, aqueous solution of potassium carbonate 4M and brine. After drying with magnesium sulfate, the organic phase is filtered and evaporated. A brownish semisolid is obtained, which weighs 0.80 g. Although with certain excess of ethyl ortoformate, 1H-RMN is consistent with the desired compound. Yield=82%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.40 (s, 3H) 2.60 (m, 6H) 2.7 (t, J=6.99 Hz, 2H) 3.35 (m, 4H) 6.80 (s, 1H) 8.45 (s, 1H)
The final product of preparation 13 (0.80 g, 2.10 mmol) is suspended in phosphorous oxychloride (20 ml) and heated to reflux for 90 min. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between chloroform and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with chloroform and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.90 g of a black oil is obtained, which is purified by flash chromatography, eluting with CH2Cl2:MeOH 95:5. 0.26 g of the final compound are isolated. The 1HNMR is consistent with the desired final product. Yield=31%.
1H NMR (200 MHz, CDCl3) δ ppm 1.15 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.40 (s, 3H) 2.65 (m, 4H) 2.75 (t, J=6.99 Hz, 2H) 3.45 (m, 6H) 9.05 (s, 1H)
The product resulting from preparation 2 (2.20 g, 8.79 mmol) is suspended in ethanol (14 ml) and pyrrolidine (4.18 ml, 50.1 mmol) is added. The reaction mixture is refluxed under nitrogen for 3 h. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting first with dichloromethane and then with the mixture CH2Cl2:MeOH 95:5.1.93 g of the final compound are obtained.
Yield=76%.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.10 (s, 6H) 1.50 (m, 2H) 2.1 (m, 6H) 2.5 (bs, 1H) 3.5 (m, 4H) 3.8 (s, 2H)
To a suspension of 3-mercapto-6,6-dimethyl-1-pyrrolidin-yl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (1.93 g, 6.71 mmol, see Preparation 15) in ethanol (100 ml), potasium carbonate (1.95 g, 14.1 mmol) and 2-chloroacetamide (0.69 g, 7.39 mmol) are added, and the reaction mixture is then refluxed for 4 h and then left overnight at room temperature. The solvent is evaporated under vacuum and water is added to the residue: the precipitated solid is filtered and dried. 1.70 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=71%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.5 (t, J=6.99 Hz, 2H) 1.90 (m, 4H) 2.65 (t, J=6.99 Hz, 2H) 3.50 (s, 2H) 3.60 (m, 4H) 5.15 (s, 2H) 6.45 (s, 2H)
1-Amino-8,8-dimethyl-5-pyrrolidin-1-yl-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamide (1.70 g, 4.93 mmol, see Preparation 16) is supended in ethyl orthoformate (35 ml) and p-toluensulfonic acid hydrate (0.07 g, 0.35 mmol) is added. This mixture is heated under reflux for 4 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 0.65 g of a black solid is filtered, but its 1HNMR is not consistent with the final structure and it is rejected. The excess of ethyl ortoformate is evaporated under vacuum and the residue is purified by flash chromatography, eluting first with dichloromethane and then with CH2Cl2:MeOH 98:2. 0.69 g of the desired final compound are isolted. Yield=39%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.0 (m, 4H) 2.8 (t, J=6.99 Hz, 2H) 3.30 (s, 2H) 3.70 (m, 4H) 8.20 (s, 1H) 12.4 (bs, 1H)
The final product of preparation 17 (0.69 g, 1.94 mmol) is suspended in phosphorous oxychloride (10 ml) and heated to reflux for 90 min. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between chloroform and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with chloroform and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.60 g of the final compound are obtained. The 1HNMR is consistent with the desired final product. Yield=83%.
1H NMR (200 MHz, CDCl3) δ ppm 1.15 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.0 (m, 4H) 2.80 (t, J=6.99 Hz, 2H) 3.35 (s, 2H) 3.75 (m, 4H) 8.90 (s, 1H)
The product resulting from preparation 2 (4.0 g, 16.0 mmol) is suspended in ethanol (4.2 ml) and dimethylamine (16.26 ml, 5.6M solution in EtOH, 91.0 mmol) is added. The reaction mixture is heated overnight at 85° C. under nitrogen in a pressure vessel. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting with the mixture CH2Cl2:MeOH 98:2. 1.54 g of the final compound are obtained.
Yield=37%.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.05 (s, 6H) 1.50 (t, J=6.56 Hz, 2H) 1.65 (bs, 1H) 2.5 (t, J=6.56 Hz, 2H) 2.6 (s, 2H) 3.1 (s, 6H)
To a suspension of 3-mercapto-1-dimethylamino-6,6-dimethyl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (1.54 g, 5.84 mmol, see Preparation 19) in ethanol (100 ml), potasium carbonate (1.94 g, 14.0 mmol) and 2-chloroacetamide (0.60 g, 6.43 mmol) are added, and the reaction mixture is then refluxed overnight. The solvent is evaporated under vacuum and water is added to the residue. After extraction with chloroform, the organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated under vacuum. 1.84 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=99%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.5 (t, J=6.99 Hz, 2H) 2.65 (t, J=6.99 Hz, 2H) 2.95 (s, 6H) 3.00 (s, 2H) 5.20 (s, 2H) 6.45 (s, 2H)
1-Amino-5-dimethylamino-8,8-dimethyl-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamide (1.84 g, 5.78 mmol, see Preparation 20) is supended in ethyl orthoformate (50 ml) and p-toluensulfonic acid hydrate (0.12 g, 0.58 mmol) is added. This mixture is heated under reflux for 4 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 1.20 g of a solid is filtered, and its 1HNMR is consistent with the final structure. The organic phase is evaporated under vacuum and the residue is purified by flash chromatography, eluting first with dichloromethane and then with CH2Cl2:MeOH 98:2. Additional 0.46 g of the desired final compound are isolted.
Global yield=87%.
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.8 (t, J=6.99 Hz, 2H) 3.0 (s, 6H) 3.35 (s, 2H) 8.20 (s, 1H) 12.1 (bs, 1H)
The final product of preparation 21 (1.20 g, 3.65 mmol) is suspended in phosphorous oxychloride (15 ml) and heated to reflux for 90 min. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between chloroform and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with chloroform and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 1.27 g of the final compound are obtained. The 1HNMR is consistent with the desired final product. Quantitative yield.
1H NMR (200 MHz, CDCl3) 8 ppm 1.1 (s, 6H) 1.6 (t, J=6.99 Hz, 2H) 2.8 (t, J=6.99 Hz, 2H) 3.0 (s, 6H) 3.40 (s, 2H) 9.01 (s, 1H)
2,2,3-Trimethyl-5-morpholin-4-yl-1,2,3,4-tetrahydropyrimido[4′, 5′:4,5]thieno[2,3-c]-2,7-naphthyridin-8(9H)-one (0.3 g, 0.78 mmol, commercially available at Pharmeks Ltd., ref.nr. PHAR010756) is suspended in phosphorous oxychloride (7 ml) and the mixture is refluxed for 90 minutes. The excess of POCl3 is evaporated under vacuum and the residue is redissolved between NaOH 2N and chloroform. The aqueous phase is extracted twice with chloroform. The organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated. 0.27 g of a brownish oil is obtained, pure enough to perform the following synthetic step. Yield=87%
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.25 (s, 6H) 2.45 (s, 3H) 3.35 (m, 4H) 3.55 (s, 2H) 3.75 (s, 2H) 3.90 (m, 4H) 9.0 (s, 1H)
A solution of chloroacetyl chloride (2.0 ml, 25.1 mmol) in dichloromethane (20 ml) is dropwise added to an ice-cooled solution of tetrahydrofurfurylamine (2.59 ml, 25.1 mmol) and trimethylamine (5.24 ml, 37.7 mmol) in dichloromethane (60 ml). Once the addition is over, the reaction mixture is allowed to stir at room temperature for 4 h. This organic solution is washed twice with water and then with brine, dried over magnesium sulphate, filtered and evaporated under vacuum. 3.39 g of the desired final product are obtained, pure enough to perform the next synthetic step. Yield=76%.
1H NMR (200 MHz, CHLOROFORM-D) δ ppm 1.55 (m, 1H) 1.98 (m, 3H) 3.25 (m, 1H) 3.6 (m, 1H) 3.8 (m, 1H) 3.90 (m, 1H) 4.05 (m, 1H) 4.10 (s, 2H) 6.90 (bs, 1H).
2-Chloro-N-(tetrahydrofuran-2-ylmethyl)-acetamide (0.50 g, 2.81 mmol, see Preparation 24) is dissolved in ethanol saturated with ammonia (15 ml) and heated to 80° C. in a sealed tube for 3.5 h. Once at room temperature, the solvent is evaporated and the residue (0.53 g, orange solid) contains the final product, pure enough to perform the following synthetic step. Yield=97%.
LRMS: m/z 158 (M+1)+
The product resulting from preparation 2 (2.20 g, 8.79 mmol) is suspended in ethanol (12 ml) and ethylmethylamine (4.52 ml, 52.7 mmol) is added. The reaction mixture is refluxed under nitrogen for 6 h. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting with the mixture CH2Cl2:MeOH 98:2. 1.20 g of the final compound are obtained.
Yield=50%.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.10 (s, 6H) 1.25 (t, 3H) 1.45-1.60 (m, 4H) 2.5 (m, 2H) 2.6 (s, 2H) 3.0 (s, 2H) 3.35 (q, 2H)
To a suspension of 1-(ethylmethylamino)-3-mercapto-6,6-dimethyl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (1.20 g, 4.36 mmol, see Preparation 27) in ethanol (25 ml), potasium carbonate (1.45 g, 10.5 mmol) and 2-chloroacetamide (0.45 g, 4.79 mmol) are added, and the reaction mixture is then refluxed for 18 h and then left overnight at room temperature. The solvent is evaporated under vacuum and water is added to the residue. After extraction with chloroform, the organic phase is washed with water and brine, dried over magnesium sulphate, filtered and evaporated under vacuum. The residue is purified by flash chromatography eluting with CH2Cl2/MeOH 98:2. 1.04 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=72%.
1H NMR (200 MHz, CDCl3) δ ppm 1.10 (s, 6H) 1.20 (t, 3H) 1.5 (m, 2H) 2.65 (t, 2H) 2.90 (s, 3H) 3.0 (s, 2H) 3.20 (q, 2H) 5.20 (bs, 1H) 6.45 (bs, 2H)
1-Amino-8,8-dimethyl-5-ethylmethylamino-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamide (1.04 g, 3.13 mmol, see Preparation 28) is supended in ethyl orthoformate (30 ml) and p-toluensulfonic acid hydrate (0.06 g, 0.31 mmol) is added. This mixture is heated under reflux for 18 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 0.62 g of a white solid is filtered, washed with Et2O and its 1HNMR is consistent with the final structure. Yield=58%.
1H NMR (200 MHz, CDCl3) 8 ppm 1.05 (s, 6H) 1.20 (t, 3H) 1.55 (t, 2H) 2.7 (t, 2H) 2.90 (s, 3H) 3.30 (m, 5H) 8.30 (s, 1H)
The final product of preparation 29 (0.62 g, 1.81 mmol) is suspended in phosphorous oxychloride (10 ml) and heated to reflux for 2 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between chloroform and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with chloroform and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.64 g of the final compound are obtained. The 1HNMR is consistent with the desired final product. Yield=98%.
1H NMR (200 MHz, CDCl3) 8 ppm 1.15 (s, 6H) 1.25 (t, 2H) 1.6 (m, 3H) 2.75 (t, 2H) 3.05 (s, 3H) 3.4 (s, 2H) 3.42 (q, 2H) 9.00 (s, 1H)
3,3-Dimethylcyclohexan-1-one (3.0 g, 23.8 mmol, synthesized according to M. G. Organ et al. J. Am. Chem. Soc., 1994, 116, 3312-3323) reacts with dimethylcarbonate (10.02 ml, 119 mmol) in tetrahydrofurane (75 ml) in the presence of sodium hydride (60%, 1.96 g, 49.0 mmol) according to the method described in L. A. Paquette J. Org. Chem., 1991, 56, 6199 to yield 4.70 g (100%) of the desired final product, pure enough to perform the next synthetic step.
1H NMR (200 MHz, CDCl3) 8 ppm 0.09 (s, 6H) 1.40 (t, 2H) 1.85 (t, 1H) 2.1 (s, 2H) 2.25 (t, 2H) 3.75 (s, 3H)
Methyl 4,4-dimethyl-2-oxo-cyclohexanecarboxylate (4.38 g, 23.8 mmol, see Preparation 30) reacts with cyanoacetamide (2.00 g, 23.8 mmol) in methanol (20 ml) in the presence of potassium hydroxide (85%, 1.40 g, 25.0 mmol) according to the method described at E. Wenkert J. Am. Chem. Soc., 1965, 87, 5461 to yield 2.75 g (yield=53%) of the desired final product.
1H NMR (300 MHz, DMSO-D6) d ppm 0.9 (s, 6H) 1.4 (t, J=6.6 Hz, 2H) 2.3 (t, J=6.6 Hz, 2H) 2.4 (s, 2H) 2.5 (m, 2H)
1,3-Dihydroxy-6,6-dimethyl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (2.75 g, 12.6 mmol, see Preparation 31) reacts with phosphorous oxychloride (8 ml) according to the method described at E. Wenkert J. Am. Chem. Soc., 1965, 87, 5461 to yield 1.97 g (yield=61%) of the desired final product.
1H NMR (300 MHz, DMSO-D6) d ppm 1.10 (s, 6H) 1.65 (t, 2H) 2.70 (s, 2H) 2.80 (m, 2H)
Palladium triphenylphosphine (0.23 g, 0.19 mmol) and potassium carbonate (0.81 g, 5.88 mmol) are suspended in dioxane (10 ml) and 1,3-dichloro-6,6-dimethyl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (0.5 g, 1.96 mmol, see Preparation 32) is added. Methylboronic acid (0.12 g, 1.96 mmol) is dropwise added at room temperature under nitrogen atmosphere, and the reaction mixture is refluxed for 48 h. The solvent is evaporated under reduced pressure and the residue is purified by flash chromatography, eluting with CH2Cl2/hexane 7:3. 0.21 g of the desired final product are obtained. Yield=45%.
1H NMR (300 MHz, DMSO-D6) d ppm 1.10 (s, 6H) 1.65 (t, 2H) 2.5 (s, 3H) 2.65 (t, 2H) 2.70 (s, 2H)
3-Chloro-1,6,6-trimethyl-5,6,7,8-tetrahydroisoquinoline-4-carbonitrile (0.21 g, 0.89 mmol, see Preparation 33) is solved in ethanol (5 ml) and potassium carbonate (0.30 g, 2.14 mmol) and thioacetamide (0.81 ml, 0.89 mmol) are added. This reaction mixture is refluxed overnight under nitrogen. Additional 0.2 equivalents of thioacetamide are added and the reflux is followed for another 4 h. Once the solvent is evaporated under reduced pressure, the residue is suspended in water and extracted with chloroform. The organic phase was washed with water and brine, dried over magnesium sulphate, filtered and evaporated to dryness. 0.26 g of the desired final product are obtained, pure enough to perform the followin sinthetic step. Yield=100%.
1H NMR (300 MHz, DMSO-D6) d ppm 1.10 (s, 6H) 1.66 (t, 2H) 2.55 (s, 3H) 2.72 (t, 2H) 3.03 (s, 2H) 5.32 (bs, 2H) 6.46 (bs, 2H)
1-Amino-5,8,8-trimethyl-6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamide (0.26 g, 0.89 mmol, see Preparation 34) is supended in ethyl orthoformate (10 ml) and p-toluensulfonic acid hydrate (0.02 g, 0.089 mmol) is added. This mixture is heated under reflux for 3 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 0.16 g of a white solid is filtered, washed with Et2O and its 1HNMR is consistent with the final structure. Yield=60%.
1H NMR (200 MHz, CDCl3) δ ppm 1.08 (s, 6H) 1.71 (t, 2H) 2.62 (s, 3H) 2.79 (t, 2H) 3.39 (s, 2H) 8.11 (s, 1H)
The final product of preparation 35 (0.16 g, 0.53 mmol) is suspended in phosphorous oxychloride (5 ml) and heated to reflux for 2 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between dichloromethane and a solution of 2N K2CO3. The aqueous phase is extracted twice with dichloromethane and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.17 g of the final compound are obtained. The 1HNMR is consistent with the desired final product. Yield=100%.
1H NMR (200 MHz, CDCl3) 8 ppm 1.10 (s, 6H) 1.75 (t, 2H) 2.68 (s, 3H) 2.83 (t, 2H) 3.47 (s, 2H) 9.10 (s, 1H)
Obtained (47%) from the title compound of Preparation 32 and isobutyl boronic acid following the experimental procedure described in Preparation 33.
1H NMR (200 MHz, CDCl3) δ ppm 0.95 (d, 6H) 1.01 (s, 6H) 1.59 (s, 2H) 1.63 (t, 2H) 2.20 (m, 1H) 2.65 (d, 2H) 2.72 (s, 2H)
Obtained (99%) from the title compound of Preparation 37 and thioacetamide following the experimental procedure described in Preparation 34.
1H NMR (200 MHz, CDCl3) δ ppm 0.97 (d, 6H) 1.05 (s, 6H) 1.63 (t, 2H) 2.21 (m, 1H) 2.72 (d, 2H) 2.80 (t, 2H) 3.04 (s, 2H) 5.32 (bs, 2H) 6.47 (bs, 2H)
Obtained (72%) from the title compound of Preparation 38 and ethyl orthoformate following the experimental procedure described in Preparation 35.
1H NMR (200 MHz, CDCl3) 8 ppm 1.00 (d, 6H) 1.01 (s, 6H) 1.70 (t, 2H) 2.29 (m, 1H) 2.80 (d, 2H) 2.88 (t, 2H) 3.42 (s, 2H) 8.29 (s, 1H) 12.57 (bs, 1H)
Obtained (68%) from the title compound of Preparation 39 and phosphorous oxychloride following the experimental procedure described in Preparation 36.
1H NMR (200 MHz, CDCl3) δ ppm 1.02 (d, 6H) 1.10 (s, 6H) 1.72 (t, 2H) 2.30 (m, 1H) 2.83 (d, 2H) 2.90 (t, 2H) 3.47 (s, 2H) 9.09 (s, 1H)
Obtained (42%) from the title compound of Preparation 32 and 2-furanboronic acid following the experimental procedure described in Preparation 33.
1H NMR (200 MHz, CDCl3) δ ppm 1.06 (s, 6H) 1.67 (t, 2H) 2.77 (s, 2H) 3.06 (t, 2H) 6.60 (m, 1H) 7.24 (m, 1H) 7.67 (s, 1H)
Obtained (92%) from the title compound of Preparation 41 and thioacetamide following the experimental procedure described in Preparation 34.
1H NMR (200 MHz, CDCl3) δ ppm 1.10 (s, 6H) 1.67 (t, 2H) 3.07 (t, 2H) 3.10 (s, 2H) 5.35 (bs, 2H) 6.51 (bs, 2H) 6.59 (d, 1H) 7.07 (d, 1H) 7.65 (s, 1H)
Obtained (97%) from the title compound of Preparation 42 and ethyl orthoformate following the experimental procedure described in Preparation 35.
1H NMR (200 MHz, CDCl3) δ ppm 1.06 (s, 6H) 1.67 (t, 2H) 3.10 (t, 2H) 3.44 (s, 2H) 6.74 (d, 1H) 7.28 (d, 1H) 7.98 (d, 1H) 8.42 (s, 1H)
Obtained (66%) from the title compound of Preparation 43 and phosphorous oxychloride following the experimental procedure described in Preparation 36.
1H NMR (200 MHz, CDCl3) 8 ppm 1.15 (s, 6H) 1.75 (t, 2H) 3.18 (t, 2H) 3.53 (s, 2H) 6.64 (d, 1H) 7.23 (d, 1H) 7.72 (s, 1H) 9.11 (s, 1H)
Obtained (61%) from the title compound of Preparation 32 and 3-furanboronic acid following the experimental procedure described in Preparation 33.
1H NMR (200 MHz, CDCl3) δ ppm 1.06 (s, 6H) 1.68 (t, 2H) 2.78 (s, 2H) 2.83 (t, 2H) 7.07 (d, 1H) 7.52 (m, 1H) 7.99 (s, 1H)
Obtained (93%) from the title compound of Preparation 45 and thioacetamide following the experimental procedure described in Preparation 34.
1H NMR (200 MHz, CDCl3) δ ppm 1.09 (s, 6H) 1.66 (t, 2H) 2.90 (t, 2H) 3.10 (s, 2H) 5.354 (bs, 2H) 6.51 (bs, 2H) 7.04 (s, 1H) 7.52 (s, 1H) 7.91 (s, 1H)
Obtained (73%) from the title compound of Preparation 46 and ethyl orthoformate following the experimental procedure described in Preparation 35.
1H NMR (200 MHz, CDCl3) δ ppm 1.12 (s, 6H) 1.71 (t, 2H) 2.97 (t, 2H) 3.47 (s, 2H) 7.11 (s, 1H) 7.55 (s, 1H) 7.98 (s, 1H) 8.13 (s, 1H)
Obtained (85%) from the title compound of Preparation 47 and phosphorous oxychloride following the experimental procedure described in Preparation 36.
1H NMR (200 MHz, CDCl3) δ ppm 1.15 (s, 6H) 1.75 (t, 2H) 3.01 (t, 2H) 3.53 (s, 2H) 7.16 (s, 1H) 7.57 (s, 1H) 8.03 (s, 1H) 9.11 (s, 1H)
In a Dean-Stark apparatus are refluxed overnight 3,3-dimethylcyclopentanone (6.18 g, 55.09 mmol, synthesized according to L. A. Paquette et al. J. Am. Chem. Soc., 1987, 109, 5731-5740), malononitrile (5.46 g, 82.64 mmol), ammonium acetate (2.76 g, 35.81 mmol) and acetic acid (5.50 ml) in toluene (80 ml). Once again at room temperature, the liquid phase is decanted and washed with water and brine, dried with magnesium sulphate, filtered and evaporated under reduced pressure. 7.32 g of the final product are obtained as an oil and pure enough to perform the next synthetic step. 1HNMR in agreement with the proposed structure. Yield=83%
1H NMR (200 MHz, CDCl3) 8 ppm 1.10 (s, 6H) 1.75 (t, 2H) 2.60 (s, 2H) 2.90 (t, 2H)
2-(3,3-Dimethylcyclopentyliden)malononitrile (7.32 g, 45.69 mmol, see Preparation 49) is solved in dimethylformamide (18.75 ml) and carbon disulfide (13.62 ml, 0.29 mol) is added in one portion. Finally, triethylamine is added (0.48 ml, 3.44 mmol). The reaction mixture is stirred at room temperature for 20 h. Water is added and the precipitated solid is filtered and washed with methanol. It weighs 3.51 g and its 1HNMR is consistent with the final product. From the aqueous phase, by extractions with ethyl ether, another 2.92 g of the final compound were isolated, pure enough to perform the next synthetic step. Global yield=60%.
1H NMR (200 MHz, CDCl3) δ ppm 1.20 (s, 6H) 1.70 (s, 2H) 1.80 (s, 2H) 3.00 (bs, 1H) 8.25 (bs, 1H)
The product resulting from preparation 50 (3.51 g, 14.85 mmol) is suspended in ethanol (3.9 ml) and dimethylamine (15.2 ml, 5.6M solution in EtOH, 84.7 mmol) is added. The reaction mixture is heated overnight at 85° C. under nitrogen in a pressure vessel. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting with the mixture CH2Cl2:MeOH 98:2. 1.21 g of the final compound are obtained.
Yield=33%.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.10 (s, 6H) 1.60 (bs, 1H) 2.70 (s, 2H) 3.10 (s, 2H) 3.2 (s, 6H)
To a suspension of 1-dimethylamino-3-mercapto-6,6-dimethyl-6,7-dihydro-5H-[2]pyridin-4-carbonitrile (1.21 g, 4.89 mmol, see Preparation 51) in ethanol (50 ml), potasium carbonate (1.35 g, 9.78 mmol) and 2-chloroacetamide (0.50 g, 5.38 mmol) are added, and the reaction mixture is then refluxed overnight. The solvent is evaporated under vacuum and water is added to the residue. After extraction with ethyl acetate, the organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated under vacuum. 1.04 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=99%.
1H NMR (200 MHz, CDCl3) δ ppm 1.20 (s, 6H) 2.85 (s, 2H) 3.00 (s, 2H) 3.10 (s, 6H) 5.25 (s, 2H) 6.15 (s, 2H)
1-Amino-5-dimethylamino-7,7-dimethyl-6H-7,8-dihydrocyclopenta[d]thieno[2,3-b]pyridin-2-carboxamide (1.04 g, 3.42 mmol, see Preparation 52) is supended in ethyl orthoformate (23 ml) and p-toluensulfonic acid hydrate (74 mg, 0.39 mmol) is added. This mixture is heated under reflux for 24 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 0.24 g of a solid is filtered, and its 1HNMR is consistent with the final structure. The organic phase is evaporated under vacuum and the residue is purified by flash chromatography, eluting first with dichloromethane/methanol 99.5:0.5 and then with CH2Cl2/MeOH 98:2. Additional 0.58 g of the desired final compound are isolted. Global yield=76%.
1H NMR (200 MHz, CDCl3) 8 ppm 1.2 (s, 6H) 2.95 (s, 2H) 3.20 (s, 6H) 3.25 (s, 2H) 8.25 (s, 1H) 12.1 (bs, 1H)
The final product of preparation 53 (0.82 g, 2.61 mmol) is suspended in phosphorous oxychloride (14 ml) and heated to reflux for 3 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between ethyl acetate and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with ethyl acetate and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.51 g of the final compound are obtained. The 1HNMR is consistent with the desired final product. Yield=59%.
1H NMR (200 MHz, CDCl3) δ ppm 1.20 (s, 6H) 3.0 (s, 2H) 3.25 (s, 6H) 3.30 (s, 2H) 8.95 (s, 1H)
The product resulting from preparation 50 (2.92 g, 12.35 mmol) is suspended in ethanol (18 ml) and morpholine (6.13 g, 70.4 mmol) is added. The reaction mixture is heated overnight at 100° C. under nitrogen. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting with the mixture CH2Cl2/MeOH 98:2. 1.70 g of the final compound are obtained.
Yield=67%.
1H NMR (300 MHz, CHLOROFORM-D) 8 ppm 1.20 (s, 6H) 2.70 (s, 2H) 2.75 (s, 2H) 3.60 (m, 4H) 3.80 (m, 4H)
To a suspension of 3-mercapto-6,6-dimethyl-1-morpholin-4-yl-6,7-dihydro-5H-[2]pyridin-4-carbonitrile (3.62 g, 12.51 mmol, see Preparation 55) in ethanol (80 ml), potasium carbonate (3.46 g, 25.03 mmol) and 2-chloroacetamide (1.28 g, 13.69 mmol) are added, and the reaction mixture is then refluxed for 48 h. The solvent is evaporated under vacuum and water is added to the residue. After usual work-up with dichloromethane, 1.57 g of a solid is obtained and its 1H-RMN is consistent with the desired product. Yield=36%.
LRMS: m/z 347 (M+1)+
1-Amino-5-(1-morfolino)-7,7-dimethyl-6H-7,8-dihydrocyclopenta[d]thieno[2,3-b]pyridin-2-carboxamide (1.57 g, 4.53 mmol, see Preparation 56) is supended in ethyl orthoformate (30 ml) and p-toluensulfonic acid hydrate (0.10 g, 0.53 mmol) is added. This mixture is heated under reflux overnight. Then the solvent is evaporated to dryness. The residue is purified by flash chromatography, eluting with CH2Cl2/MeOH 98:2. 0.19 g are obtained and its 1H-RMN is consistent with the desired compound. Yield=12%.
1H NMR (300 MHz, CHLOROFORM-D) 8 ppm 1.20 (s, 6H) 2.80 (s, 2H) 3.25 (s, 2H) 3.50 (m, 4H) 3.75 (m, 4H) 8.20 (s, 1H)
The final product of preparation 57 (0.19 g, 0.53 mmol) is suspended in phosphorous oxychloride (3 ml) and heated to reflux for 4 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between ethyl acetate and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with ethyl acetate and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.11 g of a brownish solid is obtained, whose 1HNMR is consistent with the desired final product. Yield=55%.
1H NMR (200 MHz, CDCl3) δ ppm 1.20 (s, 6H) 2.80 (s, 2H) 3.30 (s, 2H) 3.65 (m, 4H) 3.85 (m, 4H) 8.95 (s, 1H)
In a Dean-Stark apparatus are refluxed overnight 2,2-dimethylcyclopentanone (5.07 g, 45.20 mmol, commercially available at Aldrich nr.31, 147-5), malononitrile (4.48 g, 67.8 mmol), ammonium acetate (2.26 g, 29.32 mmol) and acetic acid (4.5 ml) in toluene (67 ml). The liquid phase is decanted and washed with water and brine, dried with magnesium sulphate, filtered and evaporated under reduced pressure. 6.59 g of the final product are obtained as an oil and pure enough to perform the next synthetic step. 1HNMR in agreement with the proposed structure. Yield=91%
1H NMR (200 MHz, CDCl3) δ ppm 1.40 (s, 6H) 1.80 (m, 4H) 2.90 (t, 2H)
2-(2,2-Dimethylcyclopentyliden)malononitrile (6.59 g, 41.13 mmol, see Preparation 59) is solved in dimethylformamide (17 ml) and carbon disulfide (12.26 ml, 0.26 mol) is added in one portion. Finally, triethylamine is added (0.43 ml, 3.09 mmol). The reaction mixture is stirred at room temperature for 20 h. Water is added and the precipitated solid is filtered and washed with methanol. It weighs 4.95 g and its 1HNMR is consistent with the final product. From the aqueous phase, by extractions with ethyl ether, another 4.48 g of the final compound were isolated, pure enough to perform the next synthetic step.Global yield=97%.
1H NMR (200 MHz, CDCl3) δ ppm 1.45 (s, 6H) 1.90 (t, 2H) 2.85 (t, 2H) 6.30 (bs, 1H)
The product resulting from preparation 60 (4.95 g, 20.94 mmol) is suspended in ethanol (5.5 ml) and dimethylamine (21.5 ml, 5.6M solution in EtOH, 119.36 mmol) is added. The reaction mixture is heated overnight at 85° C. under nitrogen in a pressure vessel. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting first with the mixture CH2Cl2/MeOH 99:1 and then with CH2Cl2/MeOH 98:2. 1.67 g of the final compound are obtained.
Yield=32%.
1H NMR (300 MHz, CHLOROFORM-D) 8 ppm 1.40 (s, 6H) 1.90 (t, 2H) 2.90 (t, 2H) 3.25 (s, 6H)
To a suspension of 1-dimethylamino-3-mercapto-5,5-dimethyl-6,7-dihydro-5H-[2]-pyridin-4-carbonitrile (1.67 g, 6.75 mmol, see Preparation 61) in ethanol (68 ml), potasium carbonate (1.87 g, 7.43 mmol) and 2-chloroacetamide (0.69 g, 7.43 mmol) are added, and the reaction mixture is then refluxed overnight. The solvent is evaporated under vacuum and water is added to the residue. After extraction with ethyl acetate, the organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated under vacuum. 1.70 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=83%.
1H NMR (200 MHz, CDCl3) δ ppm 1.50 (s, 6H) 2.00 (t, 2H) 3.00 (t, 2H) 3.10 (s, 6H) 5.40 (bs, 2H) 6.45 (bs, 2H)
1-Amino-5-dimethylamino-8,8-dimethyl-7,8-dihydro-6H-cyclopenta[d]thieno[2,3-b]pyridin-2-carboxamide (1.70 g, 5.58 mmol, see Preparation 62) is supended in ethyl orthoformate (38 ml) and p-toluensulfonic acid hydrate (0.12 g, 0.63 mmol) is added. This mixture is heated under reflux for 24 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 1.40 g of a solid is filtered, washed with ethyl ether, and its 1HNMR is consistent with the final structure. Yield=80%.
1H NMR (200 MHz, CDCl3) δ ppm 1.60 (s, 6H) 1.95 (t, 2H) 3.10 (s, 6H) 3.10 (t, 2H) 8.30 (s, 1H) 12.65 (bs, 1H)
The final product of preparation 63 (1.40 g, 4.45 mmol) is suspended in phosphorous oxychloride (23 ml) and heated to reflux for 4 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between ethyl acetate and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with ethyl acetate and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 1.25 g of the final compound are obtained. The 1HNMR is consistent with the desired final product. Yield=84%.
1H NMR (200 MHz, CDCl3) δ ppm 1.70 (s, 6H) 2.05 (t, 2H) 3.15 (t, 2H) 3.25 (s, 6H) 8.95 (s, 1H)
The product resulting from preparation 60 (4.48 g, 18.95 mmol) is suspended in ethanol (27 ml) and morpholine (9.41 g, 108.02 mmol) is added. The reaction mixture is heated overnight at 10° C. under nitrogen. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting with CH2Cl2/MeOH 98:2. 4.95 g of the final compound are obtained.
Yield=90%.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.40 (s, 6H) 1.90 (t, 2H) 2.80 (t, 2H) 3.15 (m, 4H) 3.90 (m, 4H) 6.30 (bs, 1H)
To a suspension of 3-mercapto-5,5-dimethyl-1-morpholin-6,7-dihydro-5H-[2]-pyridin-4-carbonitrile (4.95 g, 17.10 mmol, see Preparation 65) in ethanol (170 ml), potasium carbonate (4.73 g, 34.2 mmol) and 2-chloroacetamide (1.76 g, 18.81 mmol) are added, and the reaction mixture is then refluxed overnight. The solvent is evaporated under vacuum and water is added to the residue. After extraction with ethyl acetate, the organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated under vacuum. 1.93 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=33%.
1H NMR (200 MHz, CDCl3) δ ppm 1.50 (s, 6H) 2.05 (t, 2H) 2.85 (t, 2H) 3.40 (m, 4H) 3.85 (m, 4H) 5.30 (bs, 2H) 6.45 (bs, 2H)
1-Amino-8,8-dimethyl-5-morpholin-7,8-dihydro-6H-cyclopenta[d]thieno[2,3-b]pyridin-2-carboxamide (1.93 g, 5.57 mmol, see Preparation 66) is supended in ethyl orthoformate (38 ml) and p-toluensulfonic acid hydrate (0.12 g, 0.63 mmol) is added. This mixture is heated under reflux for 24 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 0.17 g of a light brown solid is filtered, washed with ethyl ether, but its 1HNMR is not consistent with the final structure and it is rejected. The filtrated liquid is evaporated to dryness and passed through a flash chromatography column, eluting first with CH2Cl2/MeOH 99.5:0.5 and then with CH2Cl2/MeOH 98:2. 0.43 g of the final compound are obtained. Yield=22%.
1H NMR (200 MHz, CDCl3) 8 ppm 1.70 (s, 6H) 2.05 (t, 2H) 2.95 (t, 2H) 3.50 (m, 4H) 3.85 (m, 4H) 8.25 (s, 1H) 13.05 (bs, 1H)
The final product of preparation 67 (0.43 g, 1.21 mmol) is suspended in phosphorous oxychloride (6 ml) and heated to reflux for 5 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between ethyl acetate and a cooled solution of 2N NaOH. The aqueous phase is extracted twice with ethyl acetate and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.43 g of the final compound are obtained. The 1HNMR is consistent with the desired final product. Yield=96%.
1H NMR (200 MHz, CDCl3) δ ppm 1.70 (s, 6H) 2.05 (t, 2H) 3.00 (t, 2H) 3.65 (m, 4H) 3.85 (m, 4H) 9.00 (s, 1H)
The product resulting from preparation 50 (5.0 g, 21.15 mmol) is suspended in ethanol (19 ml) and pyrrolidine (10.0 ml, 120.6 mmol) is added. The reaction mixture is heated overnight at 85° C. under nitrogen. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting first with the mixture CH2Cl2:MeOH 98:2 and then with CH2Cl2:MeOH 95:5. 2.79 g of the final compound are obtained.
Yield=48%.
LL-X00175-24
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.10 (s, 6H) 1.60 (bs, 1H) 2.10 (m, 4H) 2.70 (s, 2H) 2.90 (s, 2H) 3.7 (t, 4H)
To a suspension of 3-mercapto-6,6-dimethyl-1-pyrrolidin-1-yl-6,7-dihydro-5H-[2]pyridin-4-carbonitrile (2.55 g, 9.33 mmol, see Preparation 69) in ethanol (95 ml), potasium carbonate (2.58 g, 18.66 mmol) and 2-chloroacetamide (0.96 g, 10.26 mmol) are added, and the reaction mixture is then refluxed overnight. The solvent is evaporated under vacuum and water is added to the residue. After extraction with dichloromethane, the organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated under vacuum. 0.96 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=31%.
LL-X00175-33
1H NMR (200 MHz, CDCl3) δ ppm 1.20 (s, 6H) 1.95 (m, 4H) 3.00 (s, 4H) 3.70 (t, 4H) 5.25 (s, 2H) 6.15 (s, 2H)
1-Amino-7,7-dimethyl-5-pyrrolidin-1-yl-7,8-dihydro-6H-cyclopenta[d]thieno[2,3-b]pyridin-2-carboxamide (0.96 g, 2.91 mmol, see Preparation 70) is supended in ethyl orthoformate (19.7 ml) and p-toluensulfonic acid hydrate (60 mg, 0.32 mmol) is added. This mixture is heated under reflux for 18 h. Then the reaction mixture is allowed to reach room temperature and left overnight at +5° C. 0.58 g of a solid is filtered, and its 1HNMR is consistent with the final structure. The organic phase is evaporated under vacuum and the residue is purified by flash chromatography, eluting with dichloromethane/methanol 98:2. Additional 70 mg of the desired final compound are isolated. Global yield=66%.
LL-X00175-35
1H NMR (200 MHz, CDCl3) δ ppm 1.2 (s, 6H) 1.9 (m, 4H) 3.05 (s, 2H) 3.15 (s, 2H) 3.70 (m, 4H) 8.25 (s, 1H) 12.1 (bs, 1H)
The final product of preparation 71 (0.65 g, 1.91 mmol) is suspended in phosphorous oxychloride (10 ml) and heated to reflux for 4 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between dichloromethane and a cooled solution of 8N NaOH. The aqueous phase is extracted twice with dichloromethane and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.53 g of the final compound are obtained as a brownish solid. The 1HNMR is consistent with the desired final product. Yield=88%.
LL-X00175-37
1H NMR (200 MHz, CDCl3) δ ppm 1.20 (s, 6H) 2.00 (m, 4H) 3.05 (s, 2H) 3.25 (s, 2H) 3.90 (m, 4H) 8.95 (s, 1H)
The product resulting from preparation 50 (3.0 g, 12.69 mmol) is suspended in ethanol (17 ml) and ethylmethylamine (6.21 ml, 72.33 mmol) is added. The reaction mixture is heated overnight at 85° C. under nitrogen in a pressure vessel. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting with the mixture CH2Cl2:MeOH 98:2.1.28 g of the final compound are obtained.
Yield=39%.
LL-X00175-50
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.10 (s, 6H) 1.25 (t, 3H) 2.65 (s, 2H) 2.90 (bs, 1H) 3.10 (s, 2H) 3.18 (s, 3H) 3.5 (q, 2H)
To a suspension of 1-(ethylmethylamino)-3-mercapto-6,6-dimethyl-6,7-dihydro-5H-[2]pyridin-4-carbonitrile (1.28 g, 4.9 mmol, see Preparation 73) in ethanol (50 ml), potasium carbonate (1.35 g, 9.8 mmol) and 2-chloroacetamide (0.50 g, 5.39 mmol) are added, and the reaction mixture is then refluxed overnight. The solvent is evaporated under vacuum and water is added to the residue. A solid is formed, which is filtered and washed with Et2O. 0.60 g of the final compound are isolated. After extraction of the aqueous phase with ethyl ether, the organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated under vacuum. 0.5 g more of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=71%.
LL-X00175-52
1H NMR (200 MHz, CDCl3) δ ppm 1.20 (m, 9H) 2.80 (s, 2H) 3.00 (s, 2H) 3.10 (s, 3H) 3.50 (q, 2H) 5.25 (s, 2H) 6.15 (s, 2H)
1-Amino-7,7-dimethyl-5-ethylmethylamino-7,8-dihydro-6H-cyclopenta[d]thieno[2,3-b]pyridin-2-carboxamide (1.10 g, 3.45 mmol, see Preparation 74) is supended in ethyl orthoformate (23.3 ml) and p-toluensulfonic acid hydrate (70 mg, 0.37 mmol) is added. This mixture is heated under reflux for 18 h. The organic phase is evaporated under vacuum and the residue is purified by flash chromatography, eluting with dichloromethane/methanol 98:2. 0.69 g of the desired final compound are isolated. Global yield=61%.
LL-X00175-55
1H NMR (200 MHz, CDCl3) δ ppm 1.2 (m, 9H) 2.90 (s, 2H) 3.20 (s, 3H) 3.30 (s, 2H) 3.60 (q, 2H) 8.25 (s, 1H)
The final product of preparation 75 (0.69 g, 2.10 mmol) is suspended in phosphorous oxychloride (11 ml) and heated to reflux for 4 h. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between dichloromethane and a cooled solution of 8N NaOH. The aqueous phase is extracted twice with ethyl acetate and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 0.64 g of the final compound are obtained as a brownish solid. The 1HNMR is consistent with the desired final product.
Yield=88%.
LL-X00175-56
1H NMR (200 MHz, CDCl3) δ ppm 1.50 (s, 6H) 1.60 (t, 3H) 3.15 (s, 2H) 3.60 (m, 5H) 4.00 (q, 2H) 9.2 (s, 1H)
The product resulting from preparation 50 (2.80 g, 11.2 mmol) is suspended in ethanol (10 ml) and benzylmethylamine (8.65 ml, 67 mmol) is added. The reaction mixture is heated for 48 h at 90° C. under nitrogen in a pressure vessel. The solvent is evaporated and the residue is passed through a flash chromatography column, eluting first with dichloromethane and then with the mixture CH2Cl2:MeOH 98:2. 1.45 g of the final compound are obtained.
Yield=38%.
JT-X00248-40
LRMS: m/z 338 (M+1)+
To a suspension of 1-(benzylmethylamino)-3-mercapto-6,6-dimethyl-5,6,7,8-tetrahydro-isoquinoline-4-carbonitrile (1.45 g, 4.3 mmol, see Preparation 77) in ethanol (70 ml), potasium carbonate (1.25 g, 9.02 mmol) and 2-chloroacetamide (0.44 g, 4.73 mmol) are added, and the reaction mixture is then refluxed overnight under nitrogen. The solvent is evaporated under vacuum and water is added to the residue. After extraction of the aqueous phase with ethyl acetate, the organic phase is washed with water and brine, dried over magnesium sulfate, filtered and evaporated under vacuum. 1.70 g of the final compound are isolated. Its 1H-RMN is consistent with the proposed structure. Yield=100%.
JT-X00248-41
1H NMR (200 MHz, CDCl3) 8 ppm 1.10 (s, 6H) 1.55 (t, 2H) 2.75 (t, 2H) 2.80 (s, 3H) 3.00 (s, 2H) 4.40 (s, 2H) 5.35 (bs, 2H) 6.5 (bs, 2H) 7.40 (m, 5H)
1-Amino-5-benzylmethylamino-7,7-dimethyl-7,8-dihydro-6H-cyclopenta[d]thieno[2,3-b]pyridin-2-carboxamide (1.69 g, 4.30 mmol, see Preparation 78) is supended in ethyl orthoformate (20 ml) and p-toluensulfonic acid hydrate (81 mg, 0.43 mmol) is added. This mixture is heated under reflux for 18 h. The organic phase is evaporated under vacuum and the residue is purified by flash chromatography, eluting with dichloromethane/methanol 98:2. 1.26 g of the desired final compound are isolated. Global yield=73%.
JT-X00248-44
1H NMR (200 MHz, CDCl3) δ ppm 1.1 (s, 6H) 1.6 (m, 2H) 2.80 (t, 2H) 2.90 (s, 3H) 3.40 (s, 2H) 4.50 (s, 2H) 7.40 (m, 5H) 8.25 (s, 1H) 12.4 (bs, 1H)
The final product of preparation 79 (1.26 g, 3.11 mmol) is suspended in phosphorous-oxychloride (8 ml) and heated at 100° C. for 90 min. The excess of phosphorous oxychloride is evaporated under vacuum and the residue redissolved between chloroform and water. The aqueous phase is extracted twice with chloroform and the organic phases are washed with water and brine, dried over magnesium sulfate, filtered and the solvent evaporated. 1.27 g of the final compound are obtained as a brownish solid. The 1HNMR is consistent with the desired final product. Yield=96%.
JT-X00248-46
1H NMR (200 MHz, CDCl3) δ ppm 1.10 (s, 6H) 1.60 (t, 2H) 2.80 (m, 2H) 3.05 (s, 3H) 3.40 (s, 2H) 4.70 (s, 2H) 7.40 (m, 5H) 9.05 (s, 1H)
7-Chloro-4-benzylmethylamino-2,2-dimethyl-2,3-dihydro-1H-cyclopenta[4′,5′]pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidine (0.25 g, 0.59 mmol, see Preparation 80) is suspended in ethanol (15 ml) and (2-morpholin-4-ylethyl)amine (465 μl, 3.55 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. The solvent is evaporated under reduced pressure and the residue is purified by flash chromatography eluting first with dichloromethane/methanol 98:2 and then with dichloromethane/methanol 95:5. 203 mg of the final product have been isolated. Its 1HNMR is consistent with the desired final compound. Yield=67%.
JT-X00248-48
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.3 Hz, 2H) 2.6 (s, 4H) 2.7 (s, 2H) 2.8 (t, J=6.6 Hz, 2H) 2.9 (s, 3H) 3.5 (s, 2H) 3.7 (m, J=14.0 Hz, 6H) 4.5 (s, 2H) 5.5 (s, 1H) 7.4 (m, 5H) 8.7 (s, 1H)
Obtained (53%) from the title compound of Preparation 80 and (pyridine-3-ylmethyl)amine following the experimental procedure described in Preparation 81.
JT-X00248-50
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.0 Hz, 2H) 2.8 (m, 2H) 2.9 (s, 2H) 3.5 (s, 3H) 4.5 (s, 3H) 4.9 (d, J=5.2 Hz, 2H) 5.1 (t, J=5.8 Hz, 1H) 7.3 (m, 5H) 7.8 (d, J=7.7 Hz, 1H) 8.6 (d, J=4.4 Hz, 1H) 8.7 (s, 1H) 8.8 (s, 1H)
Obtained (70%) from the title compound of Preparation 80 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Preparation 81.
JT-X00248-49
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.6 (m, 2H) 1.9 (m, 2H) 2.1 (m, 2H) 2.5 (t, J=8.1 Hz, 2H) 2.8 (t, J=6.5 Hz, 2H) 2.9 (s, 3H) 3.4 (m, 6H) 3.7 (q, J=6.2 Hz, 2H) 4.5(s, 2H) 6.3(s, 1H) 7.4(m, 5H) 8.7(s, 1H)
8-Chloro-2,2-dimethyl-5-morpholin-4-yl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.16 g, 0.40 mmol, see Preparation 6) is suspended in ethanol (10 ml) and (2-morpholin-4-ylethyl)amine (0.27 ml, 2.02 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. At +5° C. a precipitate is formed, which is filtrated and washed with ethanol and ethyl ether. Once dried, it weighs 0.12 g and its 1HNMR is consistent with the desired final compound. Yield=62%.
m.p. 210.2-210.9° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.07 (s, 6H) 1.55 (t, J=6.10 Hz, 2H) 1.55 (t, J=6.10 Hz, 2H) 2.36 (s, 1H) 2.45 (m, 2H) 2.56 (t, J=6.87 Hz, 2H) 2.75 (t, J=6.10 Hz, 2H) 3.20 (m, 4H) 3.43 (m, 1H) 3.57 (m, 2H) 3.63 (m, 2H) 3.78 (m, 4H) 7.64 (t, J=5.49 Hz, 1H) 8.59 (s, 1H)
Obtained (45%) from the title compound of Preparation 6 and (pyridin-4-ylmethyl)amine following the experimental procedure described in Example 1.
m.p. 239.9-240.8° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.06 (s, 6H) 1.54 (t, J=5.49 Hz, 2H) 2.76 (m, 2H) 3.22 (s, 4H) 3.38 (m, 2H) 3.78 (s, 4H) 4.76 (d, J=5.49 Hz, 2H) 7.33 (d, J=4.88 Hz, 2H) 8.37 (t, J=5.49 Hz, 1H) 8.49 (d, J=5.49 Hz, 2H) 8.56 (s, 1H)
Obtained (84%) from the title compound of Preparation 6 and (pyridin-3-ylmethyl)amine following the experimental procedure described in Example 1.
m.p. 248.8-249.3° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.07 (s, 6H) 1.55 (t, J=5.80 Hz, 2H) 2.76 (s, 2H) 3.21 (s, 4H) 3.36 (m, 2H) 3.78 (s, 4H) 4.77 (d, J=5.80 Hz, 2H) 7.36 (m, 1H) 7.77 (d, J=7.94 Hz, 1H) 8.32 (d, J=6.10 Hz, 1H) 8.46 (d, J=4.88 Hz, 1H) 8.61 (m, 2H)
Obtained (82%) from the title compound of Preparation 6 and (pyridin-2-ylmethyl)amine following the experimental procedure described in Example 1.
m.p. 217.9-218.2° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.14 (s, 6H) 1.60 (m, 2H) 2.78 (t, J=6.46 Hz, 2H) 3.31 (m, 4H) 3.47 (s, 2H) 3.90 (m, 4H) 4.97 (d, J=4.67 Hz, 2H) 6.26 (d, J=4.67 Hz, 1H) 7.25 (dd, J=6.32, 3.85 Hz, 1H) 7.37 (d, J=7.69 Hz, 1H) 7.70 (m, 1H) 8.63 (d, J=5.77 Hz, 1H) 8.77 (s, 1H)
Obtained (90%) from the title compound of Preparation 6 and (2-methoxybenzyl)amine following the experimental procedure described in Example 1.
m.p. 212.4-213.1° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.13 (s, 6H) 1.60 (m, 4H) 2.77 (t, J=6.46 Hz, 2H) 3.29 (m, 4H) 3.45 (s, 2H) 3.88 (m, 2H) 3.91 (s, 3H) 4.88 (d, J=5.77 Hz, 2H) 5.31 (t, J=5.77 Hz, 1H) 6.94 (t, J=7.28 Hz, 2H) 7.30 (m, 1H) 7.39 (m, 1H) 8.75 (s, 1H)
Obtained (92%) from the title compound of Preparation 6 and (2,3-dimethoxybenzyl)amine following the experimental procedure described in Example 1.
m.p. 219.0-220.1° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.07 (s, 6H) 1.55 (t, J=6.10 Hz, 2H) 2.75 (d, J=5.19 Hz, 2H) 3.21 (s, 4H) 3.35 (m, 6H) 3.79 (s, 3H) 3.81 (s, 3H) 4.76 (d, J=5.49 Hz, 2H) 6.84 (d, J=7.02 Hz, 1H) 6.97 (m, 2H) 8.16 (t, J=5.49 Hz, 1H) 8.56 (s, 1H)
Obtained (51%) from the title compound of Preparation 6 and (4-methylamino)benzenesulfonamide following the experimental procedure described in Example 1.
m.p. 258.2-259.5° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.07 (s, 6H) 1.56 (m, 2H) 2.76 (s, 2H) 3.21 (s, 4H) 3.36 (m, 2H) 3.78 (s, 4H) 4.80 (d, J=5.77 Hz, 2H) 7.31 (s, 2H) 7.51 (d, J=8.24 Hz, 2H) 7.77 (d, J=8.52 Hz, 2H) 8.38 (s, 1H) 8.57 (s, 1H)
Obtained (83%) from the title compound of Preparation 6 and (2-pyridin-2-ylethyl)amine following the experimental procedure described in Example 1.
m.p. 237.9-239.2° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.14 (m, 6H) 1.60 (m, 4H) 2.77 (s, 2H) 3.19 (t, J=6.18 Hz, 2H) 3.31 (m, 4H) 3.90 (m, 4H) 4.06 (m, 2H) 6.33 (m, 1H) 7.19 (t, J=7.28 Hz, 2H) 7.63 (m, 1H) 8.62 (d, J=1.10 Hz, 1H) 8.73 (s, 1H)
Obtained (40%) from the title compound of Preparation 6 and (1-naphthylmethyl)amine following the experimental procedure described in Example 1.
m.p. 266.1-267.3° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.14 (s, 6H) 1.60 (m, 2H) 2.77 (t, J=6.18 Hz, 2H) 3.28 (m, 4H) 3.48 (s, 2H) 3.88 (m, 4H) 4.92 (s, 1H) 5.33 (d, J=5.22 Hz, 2H) 7.52 (m, 4H) 7.90 (m, 2H) 8.11 (dd, J=6.32, 3.30 Hz, 1H) 8.84 (s, 1H)
Obtained (62%) from the title compound of Preparation 6 and (2-furylmethyl)amine following the experimental procedure described in Example 1.
m.p. 209.6-211.0° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.14 (s, 6H) 1.60 (m, 2H) 2.77 (d, J=6.32 Hz, 2H) 3.31 (m, 4H) 3.48 (m, 2H) 3.90 (m, 4H) 4.89 (d, J=5.49 Hz, 2H) 4.97 (m, 1H) 6.37 (m, 2H) 7.42 (d, J=1.10 Hz, 1H) 8.78 (s, 1H)
Obtained (33%) from the title compound of Preparation 6 and 2-(1H-imidazol-4-yl)-ethylamine following the experimental procedure described in Example 1.
m.p. 222.7-224.1° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 1.6 (t, J=6.1 Hz, 2H) 2.7 (d, J=6.3 Hz, 2H) 2.9 (m, 2H) 3.2 (m, 5H) 3.4 (d, J=9.0 Hz, 2H) 3.8 (m, 6H) 6.9 (s, 1H) 7.5 (s, 1H) 7.8 (t, J=5.5 Hz, 1H) 8.6 (s, 1H)
Obtained (81%) from the title compound of Preparation 6 and 1-(3-aminopropyl)-pyrrolidin-2-one following the experimental procedure described in Example 1.
m.p. 209.6-210.2° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.6 (m, 2H) 1.9 (m, 2H) 2.1 (m, 2H) 2.5 (t, J=8.2 Hz, 2H) 2.8 (t, J=6.5 Hz, 2H) 3.3 (m, 4H) 3.5 (m, 6H) 3.7 (q, J=6.1 Hz, 2H) 3.9 (m, 4H) 6.3 (t, J=6.1 Hz, 1H) 8.7 (s, 1H)
8-Chloro-2,2-dimethyl-5-thiomorpholin-4-yl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.07 g, 0.17 mmol, see Preparation 10) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.11 ml, 0.86 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. At +5° C. a precipitate is formed, which is filtrated and washed with ethanol and ethyl ether. Once dried, it weighs 0.05 g and its 1HNMR is consistent with the desired final compound.
Yield=55%.
m.p. 188.2-189.3° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.13 (s, 6H) 1.60 (m, 2H) 2.56 (d, J=4.67 Hz, 2H) 2.73 (q, J=6.50 Hz, 4H) 2.85 (m, 4H) 3.45 (s, 2H) 3.54 (m, 4H) 3.74 (m, 8H) 5.56 (m, 1H) 8.73 (m, 1H)
Obtained (46%) from the title compound of Preparation 10 and (pyridine-4-ylmethyl)amine following the experimental procedure described in Example 13.
m.p. 236.8-237.4° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (m, 6H) 1.6 (m, 2H) 2.7 (t, J=6.3 Hz, 2H) 2.8 (m, 4H) 3.5 (s, 2H) 3.5 (m, 4H) 4.9 (d, J=5.9 Hz, 2H) 5.1 (t, J=6.1 Hz, 1H) 7.3 (m, 2H) 8.6 (m, 2H) 8.7 (s, 1H)
Obtained (43%) from the title compound of Preparation 10 and (pyridin-3-ylmethyl)amine following the experimental procedure described in Example 13.
m.p. 257.1-258.0° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.13 (s, 6H) 1.60 (m, 2H) 2.73 (d, J=6.04 Hz, 2H) 2.84 (m, 4H) 3.45 (s, 2H) 3.54 (m, 4H) 4.93 (d, J=5.77 Hz, 2H) 5.02 (s, 1H) 7.29 (m, 1H) 7.76 (m, 1H) 8.56 (dd, J=4.67, 1.65 Hz, 1H) 8.69 (d, J=1.65 Hz, 1H) 8.76 (s, 1H)
Obtained (84%) from the title compound of Preparation 10 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 13.
m.p. 224.7-225.8° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.6 (m, 2H) 2.7 (t, J=6.3 Hz, 2H) 2.8 (m, 4H) 3.5 (s, 2H) 3.5 (m, 4H) 5.0 (d, J=4.7 Hz, 2H) 6.3 (t, J=4.5 Hz, 1H) 7.2 (m, 1H) 7.4 (d, J=7.4 Hz, 1H) 7.7 (t, J=7.6 Hz, 1H) 8.6 (d, J=4.3 Hz, 1H) 8.8 (s, 1H)
Obtained (70%) from the title compound of Preparation 10 and (2,3-dimethoxybenzyl)amine following the experimental procedure described in Example 13.
m.p. 112.5-113.9° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.12 (s, 6H) 1.59 (m, 2H) 2.73 (m, 2H) 2.84 (m, 4H) 3.45 (s, 2H) 3.53 (m, 4H) 3.89 (s, 3H) 3.93 (s, 3H) 4.90 (d, J=5.77 Hz, 2H) 5.17 (s, 1H) 6.90 (dd, J=7.42, 2.47 Hz, 1H) 7.02 (m, 2H) 8.75 (s, 1H)
Obtained (84%) from the title compound of Preparation 10 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 13.
m.p. 219.2-220.7° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.2 (m, 6H) 1.6 (m, 2H) 1.9 (d, J=5.1 Hz, 2H) 2.1 (m, 2H) 2.5 (t, J=8.0 Hz, 2H) 2.7 (t, J=6.3 Hz, 2H) 2.8 (dd, J=4.9, 2.5 Hz, 4H) 3.4 (m, 6H) 3.5 (m, 4H) 3.7 (q, J=6.3 Hz, 2H) 6.3 (m, 1H) 8.7 (d, J=2.7 Hz, 1H)
Obtained (41%) from the title compound of Preparation 10 and ethyl 4(2-aminoethyl)piperazine-1-carboxylate following the experimental procedure described in Example 13.
m.p. 182.9-183.8° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (d, J=21.1 Hz, 6H) 1.2 (t, 3H) 1.6 (m, 4H) 2.5 (s, 4H) 2.7 (q, J=6.1 Hz, 4H) 2.8 (m, 4H) 3.5 (m, 2H) 3.5 (d, J=14.9 Hz, 6H) 3.7 (q, J=5.2 Hz, 2H) 4.2 (q, J=7.2 Hz, 2H) 5.5 (m, J=4.7 Hz, 1H) 8.7 (s, 1H)
8-Chloro-2,2-dimethyl-5-(4-methylpiperazin-yl)-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.08 g, 0.20 mmol, see Preparation 14) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.13 ml, 1.00 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. The solvent is evaporated under pressure and the residue is purified by flash chromatography eluting with CH2Cl2/MeOH 95:5. 0.05 g of the desired final product are isolated. Its 1HNMR is consistent with the desired final compound. Yield=50%.
m.p. 150-150.8° C.
1H NMR (300 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.6 (m, 2H) 2.4 (s, 3H) 2.5 (m, 3H) 2.6 (s, 4H) 2.7 (m, 4H) 3.3 (m, 4H) 3.4 (s, 2H) 3.7 (m, 7H) 5.5 (m, J=4.0, 4.0 Hz, 1H) 8.7 (s, 1H).
Obtained (46%) from the title compound of Preparation 14 and (pyridin-3-ylmethyl)amine following the experimental procedure described in Example 20.
m.p. 210.0-211.6° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.13 (s, 6H) 1.59 (t, J=6.26 Hz, 2H) 2.42 (s, 2H) 2.67 (d, J=4.27 Hz, 4H) 2.76 (t, J=6.26 Hz, 2H) 3.38 (m, 4H) 3.45 (s, 3H) 4.92 (d, J=5.80 Hz, 2H) 5.13 (t, J=5.80 Hz, 1H) 7.28 (m, 1H) 7.75 (m, 1H) 8.55 (dd, J=4.73, 1.68 Hz, 1H) 8.68 (d, J=1.83 Hz, 1H) 8.75 (s, 1H)
Obtained (80%) from the title compound of Preparation 14 and (pyridin-2-ylmethyl)amine following the experimental procedure described in Example 20.
m.p. 200.2-201.8° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.16 (m, 6H) 1.59 (m, 2H) 2.45 (s, 3H) 2.75 (m, 6H) 3.48 (m, 6H) 4.97 (d, J=4.88 Hz, 2H) 6.25 (d, J=5.80 Hz, 1H) 7.28 (m, 1H) 7.35 (s, 1H) 7.70 (m, 1H) 8.64 (s, 1H) 8.78 (m, 1H)
8-Chloro-2,2-dimethyl-5-pyrrolidin-1-yl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.08 g, 0.21 mmol, see Preparation 18) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.14 ml, 1.07 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with dichloromethane and then with CH2Cl2:MeOH 95:5.
0.05 g of the desired final compound are isolated. Yield=50%.
m.p. 173.3-174.0° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.06 (s, 6H) 1.50 (t, J=6.10 Hz, 2H) 1.90 (m, 4H) 2.44 (m, 4H) 2.56 (m, 2H) 2.77 (t, J=5.95 Hz, 2H) 3.30 (s, 2H) 3.60 (m, 10H) 7.41 (t, J=5.49 Hz, 1H) 8.52 (s, 1H)
Obtained (28%) from the title compound of Preparation 18 and (pyridin-4-ylmethyl)amine following the experimental procedure described in Example 23.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.06 (s, 6H) 1.51 (t, J=5.80 Hz, 2H) 1.90 (m, 4H) 2.78 (t, J=6.41 Hz, 2H) 3.30 (m, 2H) 3.62 (t, J=5.65 Hz, 4H) 4.74 (d, J=5.49 Hz, 2H) 7.32 (d, J=5.80 Hz, 2H) 8.16 (t, J=5.80 Hz, 1H) 8.49 (m, J=5.80 Hz, 3H)
Obtained (42%) from the title compound of Preparation 18 and (pyridin-3-ylmethyl)amine following the experimental procedure described in Example 23.
1H NMR (300 MHz, DMSO-D6) □ ppm 1.06 (s, 6H) 1.50 (t, J=6.26 Hz, 2H) 1.89 (m, 4H) 2.77 (t, J=6.10 Hz, 2H) 3.30 (m, 2H) 3.60 (m, 4H) 4.74 (d, J=5.80 Hz, 2H) 7.35 (dd, J=7.78, 4.73 Hz, 1H) 7.75 (m, 1H) 8.13 (t, J=5.95 Hz, 1H) 8.45 (dd, J=4.88, 1.53 Hz, 1H) 8.53 (s, 1H) 8.60 (d, J=1.53 Hz, 1H)
Obtained (58%) from the title compound of Preparation 18 and (pyridin-2-ylmethyl)amine following the experimental procedure described in Example 23.
m.p. 211.7-212.6° C.
1H NMR (300 MHz, DMSO-D6) 8 ppm 1.09 (m, 6H) 1.51 (s, 2H) 1.90 (s, 4H) 2.78 (m, 2H) 3.31 (m, 2H) 3.62 (s, 4H) 4.81 (d, J=6.04 Hz, 2H) 7.28 (m, 2H) 7.73 (m, 1H) 8.14 (d, J=6.32 Hz, 1H) 8.50 (m, 2H)
Obtained (66%) from the title compound of Preparation 18 and 2-methoxybenzylamine following the experimental procedure described in Example 23.
m.p. 186.4-188.9° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.06 (s, 6H) 1.50 (t, J=6.56 Hz, 2H) 1.89 (m, 4H) 2.77 (t, J=5.95 Hz, 2H) 3.31 (m, 2H) 3.61 (t, J=6.10 Hz, 4H) 3.85 (s, 3H) 4.69 (d, J=5.49 Hz, 2H) 6.86 (t, J=7.78 Hz, 1H) 7.00 (d, J=7.63 Hz, 1H) 7.13 (m, 1H) 7.22 (m, 1H) 7.92 (t, J=5.80 Hz, 1H) 8.48 (s, 1H)
Obtained (33%) from the title compound of Preparation 18 and 2,3-dimethoxybenzylamine following the experimental procedure described in Example 23.
m.p. 193.5-195.0° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.06 (s, 6H) 1.51 (d, J=1.22 Hz, 2H) 1.90 (m, 4H) 2.77 (m, 2H) 3.32 (m, 2H) 3.61 (s, 4H) 3.79 (s, 3H) 3.80 (s, 3H) 4.74 (d, J=5.80 Hz, 2H) 6.82 (m, 1H) 6.96 (m, 2H) 7.95 (s, 1H) 8.49 (s, 1H)
Obtained (27%) from the title compound of Preparation 18 and (2-furylmethyl)amine following the experimental procedure described in Example 23.
m.p. 201.0-201.6° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.6 (m, 7H) 2.0 (m, 4H) 2.8 (t, J=6.4 Hz, 3H) 3.3 (s, 2H) 3.7 (m, 4H) 8.9 (s, 1H)
Obtained (93%) from the title compound of Preparation 18 and 2-(1H-imidazol-4-yl)ethylamine following the experimental procedure described in Example 23.
m.p. 192.0-192.7° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 1.5 (t, J=6.0 Hz, 2H) 1.9 (m, 4H) 2.8 (d, J=6.2 Hz, 2H) 2.8 (s, 2H) 3.3 (m, 4H) 3.6 (m, 4H) 3.7 (m, 2H) 7.5 (s, 1H) 7.6 (t, J=5.4 Hz, 1H) 8.5 (s, 1H)
Obtained (49%) from the title compound of Preparation 18 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 23.
m.p. 199.6-200.5° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (m, 6H) 1.6 (m, 3H) 1.9 (dd, J=12.2, 6.0 Hz, 2H) 1.9 (m, 4H) 2.0 (m, 2H) 2.5 (m, 2H) 2.8 (t, J=6.4 Hz, 2H) 3.4 (m, 5H) 3.6 (m, 6H) 6.0 (t, J=6.0 Hz, 1H) 8.7 (s, 1H)
Obtained (89%) from the title compound of Preparation 18 and ethyl 4-(2-aminoethyl)-piperazine-1-carboxylate following the experimental procedure described in Example 23.
m.p. 76.2-77.8° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (d, J=6.6 Hz, 6H) 1.3 (m, 3H) 1.6 (m, 3H) 2.0 (m, 4H) 2.5 (m, 4H) 2.7 (t, J=6.0 Hz, 2H) 2.8 (t, J=6.4 Hz, 2H) 3.4 (s, 2H) 3.5 (m, 4H) 3.7 (m, 5H) 4.1 (m, 2H) 5.4 (t, J=4.6 Hz, 1H) 8.7 (s, 1H)
Ethyl 4-{2-[(2,2-dimethyl-5-pyrrolidin-1-yl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinolin-8-yl)amino]ethyl}piperazine-1-carboxylate (0.07 g, 0.13 mmol, see Example 32) is dissolved in isopropanol (3 ml). Potassium hydroxide 85% (0.09 g, 1.3 mmol) is added and the reaction mixture is refluxed overnight. The solvent is evaporated under reduced pressure and the residue is fractioned between water and chloroform. After the usual work-up, the organic residue is purified through a flash-chromatography column, eluting first with CH2Cl2/MeOH 95:5 and then with CH2Cl2/MeOH 9:1. 0.02 g of the desired final product are obtained. Yield=36%
LRMS: m/z 466 (M+1)+
Ethyl 4-{2-[(2,2-dimethyl-5-pyrrolidin-1-yl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinolin-8-yl)amino]ethyl}piperazine-1-carboxylate (0.08 g, 0.14 mmol, see Example 32) dissolved in tetrahydrofuran (2 ml) is dropwise added at room temperature to a suspension of lithium aluminium hydride (0.01 g, 0.29 mmol) in tetrahydrofuran (1 ml). After 3 h refluxing, water (50 μl), NaOH 2N (200 μl) and water (50 μl) are succsessively added. The inorganic salts are filtered through Celite®, the solvent is evaporated under reduced pressure and the residue fractioned between water and chloroform. After the usual work-up, the residue is purified through a flash-chromatography column, eluting first with CH2Cl2/MeOH 95:5 and then with CH2Cl2/MeOH 9:1. 0.02 g of the desired final product are obtained. Yield=34%
LRMS: m/z 480 (M+1)+
Obtained (29%) from the title compound of Preparation 18 and the title compound of Preparation 25 following the experimental procedure described in Example 23.
m.p. 209.7-211.3° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (m, 6H) 1.5 (m, 4H) 1.8 (m, 5H) 2.8 (t, J=6.2 Hz, 2H) 3.2 (t, J=5.8 Hz, 1H) 3.3 (s, 4H) 3.4 (d, J=7.0 Hz, 1H) 3.6 (m, 4H) 3.7 (d, J=6.2 Hz, 1H) 3.8 (t, J=6.2 Hz, 1H) 4.1 (d, J=6.2 Hz, 2H) 7.7 (s, 1H) 8.0 (m, 1H) 8.5 (s, 1H)
Obtained (54%) from the title compound of Preparation 18 and quinolin-3-ylmethylamine following the experimental procedure described in Example 23.
m.p. 226.1-226.9° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 1.5 (t, J=6.2 Hz, 2H) 1.9 (m, 4H) 2.8 (t, J=6.0 Hz, 2H) 3.3 (d, J=4.1 Hz, 2H) 3.6 (m, 4H) 4.9 (d, J=6.2 Hz, 2H) 7.6 (t, J=7.5 Hz, 1H) 7.7 (m, 1H) 8.0 (m, 2H) 8.2 (m, 2H) 8.5 (s, 1H) 9.0 (d, J=2.1 Hz, 1H)
Obtained (62%) from the title compound of Preparation 18 and isoquinolin-4-ylmethylamine following the experimental procedure described in Example 23.
m.p. 275.9-276.7° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 1.3 (m, 2H) 1.5 (t, J=6.5 Hz, 2H) 1.9 (s, 4H) 2.8 (m, 2H) 3.6 (s, 4H) 5.2 (d, J=5.9 Hz, 2H) 7.7 (m, 1H) 7.8 (m, 1H) 8.1 (s, 1H) 8.2 (d, J=8.2 Hz, 1H) 8.3 (d, J=8.2 Hz, 1H) 8.5 (s, 1H) 8.6 (s, 1H) 9.3 (s, 1H)
N5,N5,2,2-tetramethyl-N8-(2-morpholin-4-ylethyl)-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline-5,8-diamine
8-Chloro-5-dimethylamino-2,2-dimethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.07 g, 0.20 mmol, see Preparation 22) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.13 ml, 1.01 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with CH2Cl2:MeOH 95:5 and then with CH2Cl2:MeOH 98:2. 0.07 g of the desired final compound are isolated. Yield=76%.
m.p. 173.4-175.0° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.14 (s, 6H) 1.58 (m, 4H) 2.55 (m, 4H) 2.74 (m, 4H) 2.99 (s, 6H) 3.45 (m, 2H) 3.73 (m, 4H) 5.51 (m, 1H) 8.71 (s, 1H)
N5,N5,2,2-Tetramethyl-N8-(pyridin-3-ylmethyl)-1,2,3,4-tetrahydropyrimido[4′,5′:4,5] thieno[2,3-c]isoquinoline-5,8-diamine
Obtained (73%) from the title compound of Preparation 22 and (2-morpholino-4-ylethyl)amine following the experimental procedure described in Example 38.
m.p. 208.4-209.1° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.14 (s, 6H) 1.59 (m, 2H) 2.77 (t, J=6.46 Hz, 2H) 3.00 (s, 6H) 3.43 (s, 2H) 4.92 (d, J=5.77 Hz, 2H) 5.01 (m, J=5.77 Hz, 1H) 7.28 (m, 1H) 7.75 (m, 1H) 8.55 (dd, J=4.81, 1.51 Hz, 1H) 8.68 (d, J=2.20 Hz, 1H) 8.74 (s, 1H)
N8-(2,3-Dimethoxybenzyl)-N5,N5,2,2-tetramethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline-5,8-diamine
Obtained (98%) from the title compound of Preparation 22 and (2,3-dimethoxybenzyl)amine following the experimental procedure described in Example 38.
m.p. 91.9-93.0° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.13 (s, 6H) 1.58 (m, 2H) 2.76 (t, J=6.32 Hz, 2H) 2.97 (d, J=4.67 Hz, 6H) 3.43 (s, 2H) 3.88 (m, 3H) 3.93 (s, 3H) 4.90 (d, J=5.77 Hz, 2H) 5.13 (m, 1H) 6.90 (m, 1H) 7.03 (m, 2H) 8.74 (s, 1H)
Obtained (49%) from the title compound of Preparation 22 and (2,3-dimethoxybenzyl)amine following the experimental procedure described in Example 38.
m.p. 212.6-213.9° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.2 (s, 6H) 1.6 (t, J=5.7 Hz, 2H) 2.8 (d, J=6.3 Hz, 2H) 3.0 (m, 9H) 3.7 (d, J=2.0 Hz, 2H) 4.0 (d, J=6.3 Hz, 2H) 6.9 (s, 1H) 7.3 (m, J=2.0 Hz, 1H) 7.7 (s, 1H) 8.7 (d, J=1.6 Hz, 1H)
1-(3-{[5-(Dimethylamino)-2,2-dimethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinolin-8-yl]amino}propyl)pyrrolidin-2-one
Obtained (92%) from the title compound of Preparation 22 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 38.
m.p. 214.3-215.0° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (m, 6H) 1.6 (m, 2H) 1.9 (m, 2H) 2.1 (m, 2H) 2.5 (t, J=8.2 Hz, 2H) 2.8 (t, J=6.3 Hz, 2H) 3.0 (m, 6H) 3.4 (q, J=7.3 Hz, 6H) 3.7 (q, J=6.3 Hz, 2H) 6.2 (t, J=6.3 Hz, 1H) 8.7 (s, 1H)
Obtained (89%) from the title compound of Preparation 22 and ethyl 4-(2-aminoethyl)-piperazine-1-carboxylate following the experimental procedure described in Example 38.
m.p. 138.1-140.0° C.
1H NMR (400 MHz, CHLOROFORM-D) δ ppm 1.2 (m, 6H) 1.3 (t, J=7.0 Hz, 3H) 1.6 (m, 2H) 2.5 (m, 4H) 2.7 (m, 4H) 3.0 (m, 6H) 3.4 (s, 2H) 3.5 (m, 4H) 3.7 (m, 2H) 4.2 (q, J=7.0 Hz, 2H) 5.5 (t, J=4.3 Hz, 1H) 8.7 (s, 1H)
Ethyl 4-(2-{[5-(dimethylamino)-2,2-dimethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinolin-8-yl]amino}ethyl)piperazine-1-carboxylate (0.13 g, 0.26 mmol, see Example 43) is dissolved in isopropanol (5 ml). Potassium hydroxide 85% (0.17 g, 2.6 mmol) is added and the reaction mixture is refluxed overnight. The solvent is evaporated under reduced pressure and the residue is fractioned between water and chloroform. After the usual work-up, the organic residue is purified through a flash-chromatography column, eluting first with CH2Cl2/MeOH 98:2 and then with CH2Cl2/MeOH 9:1. 0.03 g of the desired final product are obtained. Yield=26%
m.p. 193.0-194.2° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (m, J=20.0 Hz, 6H) 1.6 (t, J=6.5 Hz, 3H) 2.6 (s, 4H) 2.7 (m, 4H) 3.0 (s, 6H) 3.02 (m, 4H) 3.4 (s, 2H) 3.7 (m, J=5.6, 5.6, 5.6 Hz, 2H) 5.5 (t, J=4.5 Hz, 1H) 8.7 (s, 1H)
Obtained (9%) from the title compound of Preparation 22 and 3-aminopropionitril following the experimental procedure described in Example 38.
LRMS: m/z 381 (M+1)+
Obtained (39%) from the title compound of Preparation 22 and ethanol following the experimental procedure described in Example 38.
m.p. 219.6-220.7° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.5 (m, 5H) 2.8 (t, J=6.1 Hz, 2H) 3.0 (s, 6H) 3.4 (s, 2H) 4.6 (q, J=6.8 Hz, 2H) 8.8 (m, J=1.6 Hz, 1H)
N5-Ethyl-N5,2,2-trimethyl-N8-(2-morpholin-4-ylethyl)-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline-5,8-diamine
8-Chloro-5-ethylmethylamino-2,2-dimethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.06 g, 0.17 mmol, see Preparation 29) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.13 ml, 1.01 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with CH2Cl2 and then with CH2Cl2:MeOH 99:1. 0.04 g of the desired final compound are isolated. Yield=51%.
m.p. 143.6-144.4° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (d, J=21.6 Hz, 6H) 1.2 (t, J=7.3 Hz, 3H) 1.6 (m, 5H) 2.5 (s, 2H) 2.7 (m, 3H) 3.0 (s, 3H) 3.3 (q, J=7.2 Hz, 2H) 3.4 (s, 2H) 3.7 (m, 5H) 5.5 (m, 1H) 8.7 (s, 1H)
Obtained (47%) from the title compound of Preparation 29 and pyridin-4-ylmethylamine following the experimental procedure described in Example 47.
m.p. 206.7-207.2° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.2 (m, 2H) 1.6 (m, 3H) 2.7 (t, J=6.2 Hz, 2H) 3.0 (s, 3H) 3.3 (q, J=7.0 Hz, 2H) 3.5 (m, 2H) 4.9 (d, J=6.2 Hz, 2H) 5.1 (t, J=5.6 Hz, 1H) 7.3 (d, J=6.2 Hz, 2H) 8.6 (d, J=5.8 Hz, 2H) 8.7 (s, 1H)
Obtained (71%) from the title compound of Preparation 29 and pyridin-3-ylmethylamine following the experimental procedure described in Example 47.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.3 (m, 2H) 1.6 (m, 3H) 2.7 (t, J=5.8 Hz, 2H) 3.0 (s, 3H) 3.3 (q, J=7.0 Hz, 2H) 3.4 (s, 2H) 4.9 (d, J=5.4 Hz, 2H) 5.0 (d, J=4.6 Hz, 1H) 7.3 (m, 1H) 7.8 (dd, J=7.9, 1.7 Hz, 1H) 8.6 (d, J=4.6 Hz, 1H) 8.7 (s, 1H) 8.8 (d, J=2.1 Hz, 1H)
Obtained (67%) from the title compound of Preparation 29 and pyridin-2-ylmethylamine following the experimental procedure described in Example 47.
m.p. 151.6-152.1° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.2 (t, J=7.0 Hz, 2H) 1.6 (t, 3H) 2.7 (t, J=6.4 Hz, 2H) 3.0 (s, 3H) 3.3 (q, J=7.0 Hz, 2H) 3.4 (s, 2H) 5.0 (d, J=4.6 Hz, 2 H) 6.2 (t, J=4.6 Hz, 1H) 7.2 (m, 1H) 7.4 (d, J=7.9 Hz, 1H) 7.7 (m, 1H) 8.6 (d, J=5.0 Hz, 1H) 8.7 (s, 1H)
Obtained (66%) from the title compound of Preparation 29 and furan-2-ylmethylamine following the experimental procedure described in Example 47.
m.p. 116.4-117.7° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 1.5 (t, J=6.4 Hz, 3H) 2.7 (t, J=6.2 Hz, 2H) 2.9 (s, 2H) 3.2 (q, J=7.0 Hz, 2H) 3.3 (s, 3H) 3.4 (s, 2H) 4.7 (d, J=5.8 Hz, 2H) 6.3 (d, J=2.9 Hz, 1H) 6.4 (m, 1H) 7.6 (s, 1H) 8.1 (t, J=5.6 Hz, 1H) 8.6 (s, 1H)
Obtained (32%) from the title compound of Preparation 29 and 2-(1H-imidazol-4-yl)ethylamine following the experimental procedure described in Example 47.
m.p. 202.4-203.8° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 1.2 (m, J=7.0, 7.0 Hz, 3H) 1.5 (t, J=6.2 Hz, 2H) 2.7 (t, J=6.2 Hz, 2H) 2.9 (m, 2H) 2.9 (s, 3H) 3.2 (q, J=7.3 Hz, 2H) 3.4 (s, 2H) 3.7 (m, 2H) 6.8 (s, 1H) 7.5 (s, 1H) 7.7 (t, J=5.8 Hz, 1H) 8.6 (s, 1H) 11.8 (s, 1H)
Obtained (96%) from the title compound of Preparation 29 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 47.
m.p. 166.5-167.2° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.2 (t, J=7.0 Hz, 2H) 1.6 (m, 5H) 1.9 (m, 2H) 2.5 (m, 2H) 2.7 (t, J=6.2 Hz, 2H) 2.9 (s, 3H) 3.3 (q, J=7.0 Hz, 2H) 3.4 (m, 6H) 3.7 (q, J=6.2 Hz, 2H) 6.2 (t, J=6.4 Hz, 1H) 8.7 (s, 1H)
Obtained (21%) from the title compound of Preparation 29 and 2-amino-N-(tetrahydrofuran-2-ylmethyl)acetamide (see Preparation 25) following the experimental procedure described in Example 47.
m.p. 103.1-104.8° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 1.2 (t, J=6.8 Hz, 2H) 1.5 (m, 3H) 1.8 (m, 2H) 2.7 (t, J=5.8 Hz, 2H) 2.9 (s, 2H) 3.2 (t, J=5.6 Hz, 2H) 3.3 (q, J=7.0 Hz, 2H) 3.3 (s, 3H) 3.4 (s, 2H) 3.6 (m, 1H) 3.7 (m, 1H) 3.8 (m, 1H) 4.1 (d, J=5.8 Hz, 2H) 7.9 (t, J=5.8 Hz, 1H) 8.0 (t, J=5.8 Hz, 1H) 8.5 (s, 1H)
8-Chloro-2,2,5-trimethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.05 g, 0.16 mmol, see Preparation 36) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.10 ml, 0.79 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting with CH2Cl2:MeOH 99:1. 0.06 g of the desired final compound are isolated. Yield=95%.
1H NMR (300 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.6 (s, 2H) 1.7 (t, J=6.6 Hz, 2H) 2.6 (m, 4H) 2.6 (s, 3H) 2.7 (t, J=6.0 Hz, 2H) 2.8 (t, J=6.7 Hz, 2H) 3.5 (s, 2H) 3.7 (m, 4H) 5.6 (t, J=4.4 Hz, 1H) 8.8 (s, 1H)
Obtained (66%) from the title compound of Preparation 36 and pyridin-3-ylmethylamine following the experimental procedure described in Example 55.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (m, 6H) 1.7 (t, J=6.8 Hz, 2H) 2.6 (s, 3H) 2.8 (t, J=6.7 Hz, 2H) 3.5 (s, 2H) 4.9 (d, J=6.3 Hz, 2H) 5.1 (t, J=7.0 Hz, 1H) 7.3 (dd, J=8.0, 5.3 Hz, 1H) 7.8 (d, J=8.2 Hz, 1H) 8.6 (d, J=4.3 Hz, 1H) 8.7 (s, 1H) 8.8 (s, 1H)
Obtained (72%) from the title compound of Preparation 36 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 55.
m.p. 204.8-206.3° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.6 (s, 3H) 1.9 (m, 2H) 2.1 (m, 2H) 2.5 (t, J=8.0 Hz, 2H) 2.6 (s, 3H) 2.8 (t, J=6.7 Hz, 2H) 3.4 (m, 5H) 3.7 (q, J=5.9 Hz, 2H) 6.2 (t, J=5.9 Hz, 1H) 8.7 (m, 1H)
8-Chloro-2,2-dimethyl-5-isobutyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.05 g, 0.14 mmol, see Preparation 40) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.09 ml, 0.69 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting with CH2Cl2:MeOH 99:1. 0.04 g of the desired final compound are isolated. Yield=68%.
m.p. 189.9-190.5° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.0 (d, 6H) 1.1 (s, 6H) 1.6 (s, 2H) 1.7 (t, J=6.8 Hz, 2H) 2.3 (m, 1H) 2.6 (s, 4H) 2.7 (t, J=6.1 Hz, 2H) 2.8 (d, J=7.0 Hz, 2H) 2.9 (t, J=6.5 Hz, 2H) 3.5 (s, 2H) 3.7 (dd, J=12.1, 4.7 Hz, 4H) 5.6 (m, 1H) 8.8 (s, 1H)
Obtained (89%) from the title compound of Preparation 40 and pyridin-3-ylmethylamine following the experimental procedure described in Example 58.
m.p. 229.9-230.4° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.0 (d, 6H) 1.1 (m, 6H) 1.7 (t, J=6.7 Hz, 2H) 2.3 (m, 1H) 2.8 (d, J=7.0 Hz, 2H) 2.9 (t, J=6.5 Hz, 2H) 3.5 (s, 2H) 4.9 (d, J=5.9 Hz, 2H) 5.1 (t, J=5.9 Hz, 1H) 7.3 (m, 1H) 7.7 (d, J=8.2 Hz, 1H) 8.6 (m, 1H) 8.7 (s, 1H) 8.8 (s, 1H)
Obtained (82%) from the title compound of Preparation 40 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 58.
m.p. 175.5-176.1° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.0 (d, J=6.7 Hz, 6H) 1.1 (m, J=7.8 Hz, 6H) 1.7 (t, J=6.7 Hz, 2H) 1.9 (m, 2H) 2.1 (m, 3H) 2.3 (m, 2H) 2.5 (t, J=8.2 Hz, 2H) 2.8 (d, J=7.0 Hz, 2H) 2.9 (t, J=6.7 Hz, 2H) 3.4 (m, 2H) 3.5 (s, 2H) 3.7 (q, J=6.3 Hz, 2H) 6.2 (t, J=5.9 Hz, 1H) 8.7 (s, 1H)
Obtained (39%) from the title compound of Preparation 40 and ethyl 4-(2-aminoethyl)piperazine-1-carboxylate following the experimental procedure described in Example 58.
m.p. 185.3-186.0° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.0 (d, J=6.7 Hz, 6H) 1.0 (s, 6H) 1.2 (t, J=7.0 Hz, 3H) 1.6 (t, J=6.7 Hz, 2H) 2.2 (d, J=7.0 Hz, 1H) 2.4 (t, 4H) 2.6 (t, J=6.8 Hz, 2H) 2.8 (d, J=7.0 Hz, 2H) 2.8 (t, J=6.5 Hz, 2H) 3.3 (d, 3H) 3.4 (s, 2H) 3.7 (m, 2H) 4.0 (q, J=7.0 Hz, 2H) 7.7 (t, J=5.3 Hz, 1H) 8.6 (s, 1H) 13.1 (m, 1H)
8-Chloro-5-(2-furyl)-2,2-dimethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.05 g, 0.14 mmol, see Preparation 44) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.09 ml, 0.69 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with CH2Cl2 and then with CH2Cl2:MeOH 99:1. 0.05 g of the desired final compound are isolated.
Yield=75%.
m.p. 123.0-125.1° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.7 (t, J=6.7 Hz, 2H) 2.6 (s, 4H) 2.7 (m, J=6.1, 6.1 Hz, 2H) 3.1 (t, J=6.7 Hz, 2H) 3.6 (s, 2H) 3.8 (m, 6H) 5.6 (t, J=4.3 Hz, 1H) 6.6 (dd, J=3.3, 1.8 Hz, 1H) 7.1 (d, J=3.5 Hz, 1H) 7.7 (d, J=2.0 Hz, 1H) 8.8 (s, 1H)
Obtained (38%) from the title compound of Preparation 44 and pyridin-4-ylmethylamine following the experimental procedure described in Example 62.
LRMS: m/z 442 (M+1)+
Obtained (42%) from the title compound of Preparation 44 and pyridin-3-ylmethylamine following the experimental procedure described in Example 62.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.7 (t, J=6.7 Hz, 2H) 3.1 (t, J=6.7 Hz, 2H) 3.6 (s, 2H) 4.9 (d, J=5.9 Hz, 2H) 5.1 (t, J=5.1 Hz, 1H) 6.6 (dd, J=3.3, 1.8 Hz, 1H) 7.1 (d, J=3.5 Hz, 1H) 7.3 (dd, J=8.2, 5.1 Hz, 1H) 7.7 (s, 1H) 7.8 (d, J=8.2 Hz, 1H) 8.6 (d, J=5.1 Hz, 1H) 8.7 (d, J=1.6 Hz, 1H) 8.8 (s, 1H)
Obtained (33%) from the title compound of Preparation 44 and pyridin-2-ylmethylamine following the experimental procedure described in Example 62.
m.p. 232.6-233.4° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.2 (m, 6H) 1.7 (t, J=6.7 Hz, 2H) 3.1 (t, J=6.7 Hz, 2H) 3.6 (s, 2H) 5.0 (d, J=5.1 Hz, 2H) 6.3 (t, J=4.7 Hz, 1H) 6.6 (dd, J=3.5, 2.0 Hz, 1H) 7.1 (d, J=3.1 Hz, 1H) 7.2 (m, 1H) 7.4 (d, J=7.4 Hz, 1H) 7.7 (m, 2H) 8.6 (d, J=5.1 Hz, 1H) 8.8 (s, 1H)
Obtained (72%) from the title compound of Preparation 44 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 62.
m.p. 213.7-214.2° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (d, J=16.0 Hz, 6H) 1.7 (t, J=6.7 Hz, 2H) 1.9 (m, 2H) 2.1 (m, 2H) 2.5 (t, J=8.0 Hz, 2H) 3.2 (t, J=6.8 Hz, 2H) 3.4 (m, 4H) 3.6 (s, 2H) 3.7 (q, J=6.3 Hz, 2H) 6.3 (m, 1H) 6.6 (dd, J=3.5, 1.6 Hz, 1H) 7.1 (d, J=3.5 Hz, 1H) 7.7 (s, 1H) 8.7 (s, 1H)
Obtained (42%) from the title compound of Preparation 44 and ethyl 4-(2-aminoethyl)-piperazine-1-carboxylate following the experimental procedure described in Example 62.
m.p. 99.1-100.8° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.3 (m, 3H) 2.5 (s, 4H) 2.7 (t, J=6.1 Hz, 2H) 3.1 (t, J=6.7 Hz, 2H) 3.5 (m, 8H) 3.7 (m, 2H) 4.2 (q, J=7.0 Hz, 2H) 5.6 (m, 1H) 6.6 (dd, J=3.5, 2.0 Hz, 1H) 7.1 (d, J=2.7 Hz, 1H) 7.7 (d, J=1.6 Hz, 1H) 8.8 (s, 1H)
8-Chloro-5-(3-furyl)-2,2-dimethyl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]isoquinoline (0.05 g, 0.14 mmol, see Preparation 48) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.09 ml, 0.69 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with CH2Cl2 and then with CH2Cl2:MeOH 98:2. 0.05 g of the desired final compound are isolated.
Yield=79%.
m.p. 181.7-183.1° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 2.6 (m, 4H) 2.7 (m, 2H) 3.0 (t, J=6.7 Hz, 2H) 3.5 (q, J=6.9 Hz, 2H) 3.6 (s, 2H) 3.8 (m, 6H) 5.6 (t, J=3.9 Hz, 1H) 7.1 (d, J=2.0 Hz, 1H) 7.5 (d, J=1.6 Hz, 1H) 8.0 (s, 1H) 8.8 (s, 1H)
Obtained (51%) from the title compound of Preparation 48 and pyridin-2-ylmethylamine following the experimental procedure described in Example 68.
m.p. 234.8-235.6° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.0 (s, 6H) 1.7 (t, J=6.5 Hz, 2H) 2.9 (t, J=6.3 Hz, 2H) 3.5 (s, 2H) 4.8 (d, J=5.9 Hz, 2H) 7.2 (s, 1H) 7.2 (m, 1H) 7.3 (d, J=7.8 Hz, 1H) 7.7 (m, 1H) 7.8 (s, 1H) 8.3 (s, 1H) 8.5 (m, 2H) 8.6 (s, 1H)
Obtained (83%) from the title compound of Preparation 48 and pyridin-3-ylmethylamine following the experimental procedure described in Example 68.
m.p. 247.1-248.3° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (d, J=20.3 Hz, 6H) 1.7 (t, J=6.5 Hz, 2H) 3.0 (t, J=6.5 Hz, 2H) 3.6 (s, 2H) 4.9 (d, J=6.3 Hz, 2H) 5.1 (t, J=6.3 Hz, 1H) 7.1 (d, J=2.0 Hz, 1H) 7.3 (m, 1H) 7.5 (m, 1H) 7.8 (m, 1H) 8.0 (s, 1H) 8.6 (dd, J=4.9, 1.8 Hz, 1H) 8.7 (d, J=2.0 Hz, 1H) 8.8 (s, 1H)
Obtained (59%) from the title compound of Preparation 48 and pyridin-4-ylmethylamine following the experimental procedure described in Example 68.
m.p. 264.6-266.0° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.0 (s, 6H) 1.7 (t, J=6.5 Hz, 2H) 3.0 (t, J=6.5 Hz, 2H) 3.5 (s, 2H) 4.8 (d, J=5.9 Hz, 2H) 5.7 (s, 1H) 7.2 (s, 1H) 7.3 (d, J=5.9 Hz, 2H) 7.8 (s, 1H) 8.3 (s, 1H) 8.5 (dd, J=5.9, 4.3 Hz, 2H) 8.6 (s, 1H)
Obtained (78%) from the title compound of Preparation 48 and 2-(1H-imidazol-4-yl)-ethylamine following the experimental procedure described in Example 68.
m.p. 265.3-266.6° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.0 (m, 6H) 1.7 (t, J=6.5 Hz, 2H) 2.9 (t, J=6.5 Hz, 4H) 3.5 (s, 2H) 3.7 (m, 2H) 6.9 (s, 1H) 7.1 (s, 1H) 7.5 (s, 1H) 7.8 (s, 1H) 7.9 (t, J=5.5 Hz, 1H) 8.3 (s, 1H) 8.6 (s, 1H) 11.8 (s, 1H)
Obtained (94%) from the title compound of Preparation 48 and 1-(3-aminopropyl)-pyrrolidin-2-one following the experimental procedure described in Example 68.
m.p. 193.5-194.8° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (s, 6H) 1.7 (t, J=6.7 Hz, 2H) 1.9 (d, J=5.9 Hz, 2H) 2.1 (m, 2H) 2.5 (t, J=8.2 Hz, 2H) 3.0 (t, J=6.7 Hz, 2H) 3.5 (m, 4H) 3.6 (s, 2H) 3.7 (q, J=6.3 Hz, 2H) 6.4 (m, 1H) 7.1 (s, 1H) 7.5 (t, J=1.8 Hz, 1H) 8.0 (s, 1H) 8.7 (s, 1H)
Obtained (69%) from the title compound of Preparation 48 and ethyl 4-(2-aminoethyl)-piperazine-1-carboxylate following the experimental procedure described in Example 68.
m.p. 85.1-86.4° C.
1H NMR (400 MHz, CHLOROFORM-D) d ppm 1.1 (m, 6H) 1.3 (t, J=7.0 Hz, 3H) 1.7 (t, J=6.7 Hz, 2H) 2.5 (m, 4H) 2.7 (t, J=6.1 Hz, 2H) 3.0 (t, J=6.7 Hz, 2H) 3.5 (m, 6H) 3.8 (m, 2H) 4.2 (q, J=7.2 Hz, 2H) 5.6 (t, J=4.1 Hz, 1H) 7.1 (d, J=2.0 Hz, 1H) 7.5 (m, 1H) 8.0 (s, 1H) 8.8 (s, 1H)
8-Chloro-2,2,3-trimethyl-5-morpholin-4-yl-1,2,3,4-tetrahydropyrimido[4′,5′:4,5]thieno[2,3-c]-2,7-naphthyridine (0.07 g, 0.17 mmol, see Preparation 23) is suspended in ethanol (5 ml) and (2-morpholin-4-ylethyl)amine (0.11 ml, 0.87 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with dichloromethane and then with CH2Cl2:MeOH 99:1. 0.05 g of the desired final compound are isolated. Yield=53%.
m.p. 103.2-105.1° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.13 (s, 6H) 2.31 (m, 2H) 2.50 (m, 4H) 2.57 (m, 1H) 3.20 (s, 4H) 3.33 (d, J=7.02 Hz, 5H) 3.57 (m, 4H) 3.63 (s, 4H) 3.77 (d, J=5.19 Hz, 4H) 8.59 (s, 1H)
Obtained (50%) following the experimental procedure described in Example 75 using (pyridine-3-ylmethyl)amine instead of (2-morpholino-4-ylethyl)amine.
m.p. 224.6-225.0° C.
1H NMR (300 MHz, DMSO-D6) δ ppm 1.10 (m, 6H) 2.31 (s, 3H) 3.21 (m, 4H) 3.41 (s, 2H) 3.63 (s, 2H) 3.77 (m, 4H) 4.77 (d, J=5.19 Hz, 2H) 7.35 (dd, J=7.78, 4.43 Hz, 1H) 7.77 (m, 1H) 8.36 (d, J=5.80 Hz, 1H) 8.46 (d, J=1.83 Hz, 1H) 8.60 (m, 2H)
Obtained (67%) following the experimental procedure described in Example 75 using (2-methoxybenzyl)amine instead of (2-morpholino-4-ylethyl)amine.
m.p. 73.8-75.9° C.
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.28 (d, J=13.12 Hz, 6H) 2.48 (d, J=5.49 Hz, 2H) 3.26 (m, 4H) 3.64 (s, 2H) 3.85 (m, 7H) 3.90 (m, 3H) 4.86 (dd, J=11.14, 6.26 Hz, 2H) 5.33 (m, J=5.49 Hz, 1H) 6.92 (m, 2H) 7.30 (m, 1H) 7.39 (d, J=7.63 Hz, 1H) 8.73 (s, 1H)
7-Chloro-N,N,2,2-tetramethyl-2,3-dihydro-1H-cyclopenta[4′,5′]pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4-amine (0.1 g, 0.30 mmol, see Preparation 54) is suspended in ethanol (7 ml) and (2-morpholin-4-ylethyl)amine (0.20 ml, 1.50 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with CH2Cl2:MeOH 99.5:0.5 and then with CH2Cl2:MeOH 98:2. 0.10 g of the desired final compound are isolated. Yield=77%.
m.p. 174.9-176.0° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (s, 6H) 2.4 (m, 4H) 2.5 (m, 2H) 2.9 (s, 2H) 3.19 (s, 2H) 3.3 (s, 6H) 3.6 (m, 6H) 7.5 (s, 1H) 8.5 (s, 1H)
Obtained (83%) from the title compound of Preparation 54 and pyridin-3-ylmethylamine following the experimental procedure described in Example 78.
m.p. 248.6-249.4° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.2 (s, 6H) 2.9 (s, 2H) 3.1 (s, 6H) 3.2 (d, J=7.0 Hz, 2H) 4.7 (d, J=5.9 Hz, 2H) 7.3 (dd, J=7.8, 4.7 Hz, 1H) 7.8 (m, 1H) 8.2 (d, J=5.5 Hz, 1H) 8.4 (dd, J=4.7, 1.6 Hz, 1H) 8.5 (s, 1H) 8.6 (s, 1H)
Obtained (93%) from the title compound of Preparation 54 and 1-(3-aminopropyl)-pyrrolidin-2-one following the experimental procedure described in Example 78.
m.p. 184.4-185.7° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.2 (s, 6H) 1.8 (m, 2H) 1.9 (m, 2H) 2.2 (t, J=8.0 Hz, 2H) 3.0 (m, 2H) 3.1 (m, 6H) 3.2 (m, 2H) 3.3 (t, J=7.0 Hz, 2H) 3.4 (m, 2H) 3.5 (t, 2H) 7.5 (t, J=5.7 Hz, 1H) 8.5 (s, 1H)
Obtained (64%) from the title compound of Preparation 54 and furyl-2-ylmethylamine following the experimental procedure described in Example 78.
m.p. 216.6-217.3° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.1 (d, J=20.0 Hz, 6H) 2.9 (s, 2H) 3.1 (m, J=20.0 Hz, 6H) 3.2 (m, J=10.6 Hz, 2H) 4.7 (d, J=5.5 Hz, 2H) 6.3 (d, J=2.7 Hz, 1H) 6.4 (m, 1H) 7.6 (s, 1H) 8.1 (t, J=5.9 Hz, 1H) 8.5 (s, 1H)
Obtained (46%) from the title compound of Preparation 58 and (pyridin-3-ylmethyl)amine following the experimental procedure described in Example 1.
m.p. 212.7-214.0° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.2 (s, 6H) 2.8 (s, 2H) 3.3 (s, 2H) 3.5 (m, 4H) 3.7 (m, 4H) 4.8 (d, J=5.9 Hz, 2H) 7.3 (dd, J=7.8, 4.7 Hz, 1H) 7.8 (d, J=7.8 Hz, 1H) 8.3 (t, J=6.1 Hz, 1H) 8.4 (dd, J=4.7, 1.6 Hz, 1H) 8.5 (s, 1H) 8.6 (d, J=2.3 Hz, 1H)
7-Chloro-N,N,1,1-tetramethyl-2,3-dihydro-1H-cyclopenta[4′,5′]pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4-amine (0.2 g, 0.60 mmol, see Preparation 64) is suspended in ethanol (14 ml) and (2-morpholin-4-ylethyl)amine (0.39 ml, 3.00 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with CH2Cl2/MeOH 99.5:0.5 and then with CH2Cl2/MeOH 99:1. 0.06 g of the desired final compound are isolated. Yield=88%.
m.p. 197.3-198.5° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.7 (m, 6H) 2.0 (t, J=7.4 Hz, 2H) 2.5 (m, 4H) 2.6 (m, 2H) 3.1 (m, 6H) 3.3 (m, J=18.0 Hz, 2H) 3.6 (m, 6H) 7.4 (t, J=5.7 Hz, 1H) 8.5 (s, 1H)
Obtained (88%) from the title compound of Preparation 64 and (pyridin-3-ylmethyl)amine following the experimental procedure described in Example 83.
m.p. 237.1-237.7° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.6 (d, J=20.0 Hz, 6H) 2.0 (t, J=7.4 Hz, 2H) 3.3 (s, 2H) 3.3 (s, 6H) 4.8 (d, J=5.9 Hz, 2H) 7.3 (dd, J=7.8, 4.7 Hz, 1H) 7.8 (m, 1H) 8.1 (t, J=5.9 Hz, 1H) 8.4 (m, 1H) 8.5 (s, 1H) 8.6 (s, 1H)
Obtained (37%) from the title compound of Preparation 64 and ethyl 4-(2-aminoethyl)-piperazine-1-carboxylate following the experimental procedure described in Example 83.
m.p. 214.7-215.6° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.2 (m, 6H) 2.0 (m, 2H) 2.4 (m, 6H) 2.6 (t, J=6.8 Hz, 2H) 3.1 (m, 6H) 3.3 (m, 6H) 3.6 (q, J=6.3 Hz, 2H) 4.0 (q, J=7.2 Hz, 2H) 7.4 (t, J=5.7 Hz, 1H) 8.5 (s, 1H) 13.1 (s, 1H)
Obtained (42%) from the title compound of Preparation 64 and 1-(3-aminopropyl)pyrrolidin-2-one following the experimental procedure described in Example 83.
m.p. 194.1-195.7° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.7 (s, 6H) 1.8 (m, 2H) 1.9 (m, 4H) 2.2 (t, J=8.0 Hz, 2H) 3.1 (m, 6H) 3.3 (t, J=7.0 Hz, 2H) 3.4 (m, 3H) 3.5 (m, 2H) 7.5 (t, J=5.7 Hz, 1H) 8.5 (s, 1H) 13.1 (s, 1H)
Obtained (88%) from the title compound of Preparation 64 and 2-(1H-imidazol-4-yl)-ethylamine following the experimental procedure described in Example 83.
m.p. 260.8-261.5° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.7 (s, 6H) 1.9 (t, J=7.4 Hz, 2H) 2.8 (m, 2H) 3.1 (m, J=5.1 Hz, 2H) 3.3 (s, 6H) 3.7 (m, 2H) 6.8 (s, 1H) 7.5 (s, 1H) 7.6 (t, J=5.5 Hz, 1H) 8.5 (s, 1H) 11.8 (s, 1H)
Obtained (67%) from the title compound of Preparation 64 and furyl-2-ylmethylamine following the experimental procedure described in Example 83.
m.p. 89.3-91.0° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.6 (m, 6H) 2.0 (m, 2H) 3.1 (m, 6H) 3.3 (d, J=17.0 Hz, 2H) 4.7 (d, J=5.8 Hz, 2H) 6.3 (d, J=3.3 Hz, 1H) 6.4 (m, 1H) 7.6 (s, 1H) 8.0 (m, J=5.8, 5.8 Hz, 1H) 8.6 (s, 1H)
Obtained (42%) from the title compound of Preparation 64 and 2,3-dimethoxybenzylamine following the experimental procedure described in Example 83.
m.p. 91.9-93.6° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.7 (s, 6H) 2.0 (t, J=7.4 Hz, 2H) 3.1 (m, 2H) 3.1 (s, 6H) 3.8 (s, 3H) 3.8 (s, 3H) 4.8 (d, J=5.1 Hz, 2H) 6.9 (m, J=5.9 Hz, 4H) 8.5 (s, 1H)
7-Chloro-1,1-dimethyl-4-morpholin-4-yl-2,3-dihydro-1H-cyclopenta[4′,5′]pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4-amine (0.11 g, 0.29 mmol, see Preparation 68) is suspended in ethanol (7 ml) and (2-morpholin-4-ylethyl)amine (0.19 ml, 1.45 mmol) is added. The mixture is refluxed overnight and then allowed to cool to room temperature. After evaporation of the solvent under vacuum, the residue is purified by flash chromatography, eluting first with CH2Cl2/MeOH 99.5:0.5 and then with CH2Cl2/MeOH 99:1 and finally with CH2Cl2/MeOH 98:2. 0.11 g of the desired final compound are isolated as a solid. Yield=81%.
m.p. 100.0-100.9° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.7 (s, 6H) 2.0 (t, J=7.3 Hz, 2H) 2.4 (d, J=4.1 Hz, 2H) 2.6 (t, 2H) 3.0 (t, J=7.3 Hz, 2H) 3.4 (m, 4H) 3.6 (m, 4H) 3.6 (m, 4H) 3.7 (m, 4H) 7.5 (t, J=5.4 Hz, 1H) 8.6 (s, 1H)
Obtained (100%) from the title compound of Preparation 68 and pyridin-3-ylmethylamine following the experimental procedure described in Example 90.
m.p. 185.5-186.8° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.7(s, 6H) 2.0 (t, J=7.5 Hz, 2H) 3.0 (t, J=7.3 Hz, 2H) 3.4 (m, 4H) 3.7 (m, 4H) 4.8 (d, J=6.2 Hz, 2H) 7.3 (dd, J=7.9, 5.0 Hz, 1H) 7.8 (d, J=7.9 Hz, 1H) 8.3 (t, J=6.2 Hz, 1H) 8.5 (m, 1H) 8.6 (s, 1H) 8.6 (s, 1H)
Obtained (64%) from the title compound of Preparation 68 and 1-(3-aminopropyl)-pyrrolidin-2-one following the experimental procedure described in Example 90.
m.p. 141.8-143.0° C.
1H NMR (400 MHz, DMSO-D6) d ppm 1.7 (s, 6H) 1.8 (m, 2H) 1.9 (m, 2H) 2.0 (t, J=7.3 Hz, 2H) 2.2 (t, J=8.1 Hz, 2H) 3.0 (t, J=7.3 Hz, 2H) 3.3 (t, J=7.3 Hz, 2H) 3.4 (m, 2H) 3.4 (m, 4H) 3.5 (m, 2H) 3.7 (t, 4H) 7.6 (t, J=5.6 Hz, 1H) 8.6 (s, 1H)
Obtained (71%) from the title compound of Preparation 72 and (2-morpholine-4-ylethyl)amine following the experimental procedure described in Example 78.
LAS100506
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (s, 6H) 2.0 (m, 4H) 2.5 (m, 4H) 2.7 (t, J=5.9 Hz, 2H) 3.0 (s, 2H) 3.3 (s, 2H) 3.7 (m, 10H) 5.5 (t, J=4.5 Hz, 1H) 8.7 (s, 1H)
Obtained (25%) from the title compound of Preparation 72 and pyridine-3-ylmethylamine following the experimental procedure described in Example 78.
LAS100513
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (m, 6H) 2.0 (t, J=6.5 Hz, 4H) 3.1 (s, 2H) 3.3 (s, 2H) 3.8 (m, 4H) 4.9 (d, J=5.8 Hz, 2H) 5.0 (m, 1H) 7.3 (m, 1H) 7.8 (d, J=8.2 Hz, 1H) 8.6 (d, J=4.1 Hz, 1H) 8.7 (m, 1H) 8.7 (s, 1H)
Obtained (12%) from the title compound of Preparation 72 and 2-(2-morpholin-4-ylethylamino)ethanol following the experimental procedure described in Example 78.
LAS100529
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (s, 6H) 2.0 (m, 4H) 2.5 (m, 6H) 2.8 (s, 2H) 3.1 (s, 2H) 3.3 (s, 2H) 3.6 (m, 2H) 3.7 (m, 7H) 4.0 (m, 2H) 4.0 (s, 2H) 8.6 (s, 1H)
Obtained (39%) from the title compound of Preparation 18 and 2-(2-morpholin-4-ylethylamino)ethanol following the experimental procedure described in Example 23.
LAS100545
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.6 (m, J=6.9 Hz, 2H) 2.0 (m, 4H) 2.6 (m, 4H) 2.8 (t, J=5.9 Hz, 2H) 2.9 (m, 2H) 3.4 (s, 2H) 3.7 (m, 4H) 3.7 (m, 4H) 4.0 (m, 6H) 8.6 (s, 1H)
Obtained (15%) from the title compound of Preparation 72 and dimethylamine (dissolved in dimethylformamide) following the experimental procedure described in Example 78.
LAS100547
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (s, 6H) 2.0 (t, J=6.3 Hz, 4H) 3.0 (s, 2H) 3.3 (s, 2H) 3.4 (s, 6H) 3.7 (t, J=6.3 Hz, 4H) 8.6 (s, 1H)
Obtained (10%) from the title compound of Preparation 72 and (2-morpholin-4-ylethyl)-pyridin-3-ylmethylamine following the experimental procedure described in Example 78.
LAS100550
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.3 (m, 6H) 2.0 (t, J=6.3 Hz, 4H) 2.5 (d, J=3.8 Hz, 4H) 2.7 (t, J=6.7 Hz, 2H) 3.1 (s, 2H) 3.3 (s, 2H) 3.7 (m, 8H) 3.9 (t, J=6.7 Hz, 2H) 5.2 (s, 2H) 7.2 (m, 1H) 7.7 (d, J=8.0 Hz, 1H) 8.6 (m, 3H)
Obtained (43%) from the title compound of Preparation 76 and (2-morpholin-4-ylethyl)amine following the experimental procedure described in Example 78.
LAS100582
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (m, 9H) 2.5 (m, 4H) 2.7 (t, J=6.0 Hz, 2H) 2.9 (s, 2H) 3.2 (s, 3H) 3.3 (s, 2H) 3.6 (q, J=7.0 Hz, 2H) 3.7 (m, 6H) 5.5 (m, 1H) 8.7 (s, 1H)
Obtained (21%) from the title compound of Preparation 76 and pyridine-3-ylmethylamine following the experimental procedure described in Example 78.
LAS100586
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (s, 9H) 2.9 (s, 2H) 3.2 (s, 3H) 3.3 (s, 2H) 3.6 (q, J=7.1 Hz, 2H) 4.9 (d, J=5.8 Hz, 2H) 5.1 (d, J=5.2 Hz, 1H) 7.3 (m, 2H) 7.8 (d, J=8.2 Hz, 1H) 8.6 (s, 1H) 8.7 (s, 1H)
Obtained (71%) from the title compound of Preparation 76 and 1-(3-aminopropyl)-pyrrolidine-2-one following the experimental procedure described in Example 78.
LAS100589
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (m, 9H) 1.9 (dd, J=11.5, 5.5 Hz, 2H) 2.1 (m, 2H) 2.5 (t, J=8.1 Hz, 2H) 2.9 (s, 2H) 3.1 (s, 3H) 3.3 (s, 2H) 3.4 (m, 4H) 3.6 (m, 4H) 6.2 (m, 1H) 8.7 (s, 1H)
Obtained (51%) from the title compound of Preparation 76 and 2,3-dimethoxybenzylamine following the experimental procedure described in Example 78.
LAS100596
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (m, 9H) 2.9 (s, 2H) 3.1 (s, 3H) 3.3 (s, 2H) 3.6 (q, J=7.0 Hz, 2H) 3.9 (s, 3H) 3.9 (s, 3H) 4.9 (d, J=5.5 Hz, 2H) 5.1 (t, J=5.6 Hz, 1H) 6.9 (dd, J=7.1, 2.7 Hz, 1H) 7.0 (m, 2H) 8.7 (s, 1H)
Obtained (26%) from the title compound of Preparation 76 and 2-(2-morpholin-4-ylethylamino)ethanol following the experimental procedure described in Example 78.
LAS100599
1H NMR (300 MHz, DMSO-D6) δ ppm 1.2 (s, 9H) 2.5 (s, 4H) 2.6 (t, J=7.0 Hz, 2H) 2.9 (s, 2H) 3.1 (s, 3H) 3.2 (s, 2H) 3.6 (m, 5H) 3.7 (m, 4H) 3.8 (m, 2H) 3.9 (m, 2H) 8.5 (s, 1H)
N4-Ethyl-N4,2,2-trimethyl-N-7-(2-morpholin-4-ylethyl)-N7-(pyridin-3-ylmethyl)-2,3-dihydro-1H-cyclopenta[4′,5′]pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4,7-diamine
Obtained (8%) from the title compound of Preparation 76 and (2-morpholin-4-ylethyl)-pyridin-3-ylmethylamine following the experimental procedure described in Example 78.
LAS100600
1H NMR (300 MHz, DMSO-D6) δ ppm 1.1 (m, 9H) 2.4 (m, 4H) 2.5 (m, 2H) 2.6 (t, J=6.9 Hz, 2H) 2.9 (s, 2H) 3.1 (s, 3H) 3.2 (s, 2H) 3.5 (m, 4H) 3.9 (m, 2H) 5.1 (s, 2H) 7.3 (dd, J=7.8, 4.5 Hz, 1H) 7.7 (d, J=8.0 Hz, 1H) 8.5 (dd, J=4.8, 1.8 Hz, 1H) 8.5 (s, 1H) 8.5 (d, J=1.6 Hz, 1H)
Obtained (39%) from the title compound of Preparation 58 and (2-morpholin-4-ylethyl)amine following the experimental procedure described in Example 78.
LAS100629
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (m, 6H) 2.6 (s, 4H) 2.7 (t, J=5.9 Hz, 2H) 2.8 (s, 2H) 3.4 (s, 2H) 3.6 (m, 4H) 3.7 (m, 6H) 3.9 (m, 4H) 5.6 (m, 1H) 8.7 (s, 1H)
Obtained (51%) from the title compound of Preparation 58 and 2-(2-morpholin-4-ylethylamino)ethanol following the experimental procedure described in Example 78.
LAS100637
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (s, 6H) 2.6 (s, 5H) 2.8 (s, 2H) 2.9 (t, J=5.1 Hz, 2H) 3.4 (s, 2H) 3.6 (m, 4H) 3.7 (m, 4H) 3.9 (m, 4H) 4.0 (m, 6H) 8.6 (s, 1H)
Obtained (73%) from the title compound of Preparation 58 and 2,3-dimethoxybenzylamine following the experimental procedure described in Example 78.
LAS100644
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (s, 6H) 2.8 (s, 2H) 3.4 (s, 2H) 3.5 (m, 3H) 3.9 (m, 5H) 3.9 (s, 3H) 3.9 (s, 3H) 4.9 (d, J=5.5 Hz, 2H) 5.2 (t, J=5.6 Hz, 1H) 6.9 (dd, J=7.1, 2.5 Hz, 1H) 7.0 (m, 2H) 8.7 (s, 1H)
Obtained (73%) from the title compound of Preparation 58 and 1-(3-aminopropyl)-pyrrolidin-2-one following the experimental procedure described in Example 78.
LAS100649
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.2 (s, 6H) 1.9 (m, 2H) 2.1 (m, 2H) 2.5 (t, J=8.1 Hz, 2H) 2.8 (s, 2H) 3.4 (s, 2H) 3.4 (m, 4H) 3.5 (m, 4H) 3.6 (m, 2H) 3.9 (m, 4H) 6.4 (t, J=6.0 Hz, 1H) 8.7 (s, 1H)
Obtained (57%) from the title compound of Preparation 6 and 2-(2-morpholin-4-ylethylamino)ethanol following the experimental procedure described in Example 1.
LAS100690
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.3 Hz, 2H) 2.6 (s, 4H) 2.8 (t, J=6.3 Hz, 2H) 2.9 (m, 2H) 3.3 (m, 4H) 3.5 (s, 3H) 3.7 (m, 4H) 3.9 (m, 4H) 4.0 (m, 6H) 8.6 (s, 1H)
Obtained (46%) from the title compound of Preparation 6 and [(pyridin-3-ylmethyl)amino]ethanol following the experimental procedure described in Example 1.
LAS100718
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.6 (t, J=6.2 Hz, 2H) 2.4 (s, 2H) 2.8 (t, J=5.9 Hz, 2H) 3.3 (m, 4H) 3.5 (s, 2H) 3.9 (m, 3H) 3.9 (m, 3H) 4.4 (s, 1H) 5.2 (s, 2H) 7.3 (m, 1H) 7.7 (m, 1H) 8.5 (m, 2H) 8.7 (s, 1H)
N5-Benzyl-N5,2,2-trimethyl-N8-(2-morpholin-4-ylethyl)-1,2,3,4-tetrahydropyrimido [4′,5′:4,5]thieno[2,3-c]isoquinoline-5,8-diamine (173 mg, 0.33 mmol, see Preparation 81) is dissolved in toluene and aluminum chloride is portionwise added. This reaction mixture is refluxed for 2 h. Once the reaction is over, the reaction mixture is diluted with ethyl acetate and washed twice with water, once with brine, dried over magnesium sulfate, filtered and evaporated under reduced pressure. The residue is purified by flash chromatography, eluting first with dichloromethane, then with dichloromethane/methanol 99:1 and finally with dichloromethane/methanol 98:2. 73 mg of the final compound are isolated. Its 1HNMR is consistent with the desired final compound. Yield=51%.
LAS100851
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.7 (t, J=5.9 Hz, 2H) 2.4 (t, J=6.3 Hz, 2H) 2.5 (s, 4H) 2.7 (t, J=4.7 Hz, 2H) 3.2 (d, J=3.3 Hz, 3H) 3.4 (s, 2H) 3.7 (m, J=16.5 Hz, 6H) 4.7 (s, 1H) 5.5 (s, 1H) 8.7 (s, 1H)
Obtained (74%) from the title compound of Preparation 82 following the experimental procedure described in Example 111.
LAS100852
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.7 (t, J=6.3 Hz, 2H) 2.4 (t, J=6.2 Hz, 2H) 3.2 (d, J=4.7 Hz, 3H) 3.4 (s, 2H) 4.7 (d, J=4.4 Hz, 1H) 4.9 (d, J=5.5 Hz, 2H) 5.0 (d, J=8.2 Hz, 1H) 7.3 (m, 1H) 7.8 (d, J=7.7 Hz, 1H) 8.6 (d, J=4.4 Hz, 1H) 8.7 (s, 1H) 8.7 (s, 1H)
Obtained (61%) from the title compound of Preparation 83 following the experimental procedure described in Example 111.
LAS100883
1H NMR (300 MHz, CHLOROFORM-D) δ ppm 1.1 (s, 6H) 1.7 (t, J=6.6 Hz, 2H) 1.9 (m, 2H) 2.1 (m, 2H) 2.4 (m, 4H) 3.1 (d, J=4.9 Hz, 3H) 3.4 (s, 2H) 3.4 (m, 4H) 3.6 (q, J=6.0 Hz, 2H) 4.6 (m, J=4.4 Hz, 1H) 6.0 (m, 1H) 8.7 (s, 1H)
The following examples illustrate pharmaceutical compositions according to the present invention.
Using a mixer machine, 15 g of the compound of the present invention are mixed with 340.8 g of lactose and 85.2 g of microcrystalline cellulose. The mixture is subjected to compression moulding using a roller compactor to give a flake-like compressed material. The flake-like compressed material is pulverised using a hammer mill, and the pulverised material is screened through a 20 mesh screen. A 4.5 g portion of light silicic anhydride and 4.5 g of magnesium stearate are added to the screened material and mixed. The mixed product is subjected to a tablet making machine equipped with a die/punch system of 7.5 mm in diameter, thereby obtaining 3,000 tablets each having 150 mg in weight.
Using a fluidised bed granulating machine, 15 g of the compound of the present invention are mixed with 285.6 g of lactose and 122.4 g of corn starch. Separately, 22.5 g of polyvinylpyrrolidone is dissolved in 127.5 g of water to prepare a binding solution. Using a fluidised bed granulating machine, the binding solution is sprayed on the above mixture to give granulates. A 4.5 g portion of magnesium stearate is added to the obtained granulates and mixed. The obtained mixture is subjected to a tablet making machine equipped with a die/punch biconcave system of 6.5 mm in diameter, thereby obtaining 3,000 tablets, each having 150 mg in weight.
Separately, a coating solution is prepared by suspending 6.9 g of hydroxypropylmethyl-cellulose 2910, 1.2 g of polyethylene glycol 6000, 3.3 g of titanium dioxide and 2.1 g of purified talc in 72.6 g of water. Using a High Coated, the 3,000 tablets prepared above are coated with the coating solution to give film-coated tablets, each having 154.5 mg in weight.
25 g of active compound, 1 Kg of lactose monohydrate, 10 g of colloidal silicon dioxide, 100 g of corn starch and 20 g of magnesium stearate are mixed. The mixture is sieved through a 60 mesh sieve, and then filled into 5,000 gelatine capsules.
An oil-in-water emulsion cream is prepared with the ingredients listed above, using conventional methods.
| Number | Date | Country | Kind |
|---|---|---|---|
| P200402876 | Nov 2004 | ES | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP05/12774 | 11/30/2005 | WO | 00 | 1/3/2008 |
