Patent Application
20080045506
The present invention relates to novel pyrrolidine derivatives having pharmacological activity, processes for their preparation, to compositions containing them and to their use in the treatment of neurological and psychiatric disorders.
WO2004/101546 (Glaxo Group Ltd; published 25 Nov. 2004) describes a series of piperazine derivatives and their use in the treatment of neurological disorders. JP 10130241 (Wakunaga Seiyaku KK) describes a series of pyridine carboxylic acid derivatives which are claimed to be useful as platelet coagulation inhibitors for treating peripheral circulation disorders, as metastasis inhibitors of malignant tumour or as anti-inflammatory agents. WO 97/06802 (Zeneca Ltd.) discloses a series of heterocyclic derivatives as oxido-squalene cyclase inhibitors. WO2003062234 (Yamanouchi Pharmaceutical Co. Ltd.) describes quinoxaline derivatives which are stated to be poly(ADP-ribose) polymerase inhibitors, and the use of these compounds in the treatment of arthritis. WO 02/072570 (Schering Corporation and Pharmacopeia, Inc.) discloses a series of compounds that are useful in the treatment of allergies and CNS disturbances. WO 2004/000831 (Schering Corporation) describes a series of indole derivatives which are stated to be H3 antagonists. The use of these compounds in the treatment of CNS disturbances is also described.
The histamine H3 receptor is predominantly expressed in the mammalian central nervous system (CNS), with minimal expression in peripheral tissues except on some sympathetic nerves (Leurs et al., (1998), Trends Pharmacol. Sci. 19, 177-183). Activation of H3 receptors by selective agonists or histamine results in the inhibition of neurotransmitter release from a variety of different nerve populations, including histaminergic and cholinergic neurons (Schlicker et al., (1994), Fundam. Clin. Pharmacol. 8, 128-137). Additionally, in vitro and in vivo studies have shown that H3 antagonists can facilitate neurotransmitter release in brain areas such as the cerebral cortex and hippocampus, relevant to cognition (Onodera et al., (1998), In: The Histamine H3 receptor, ed Leurs and Timmerman, pp 255-267, Elsevier Science B.V.). Moreover, a number of reports in the literature have demonstrated the cognitive enhancing properties of H3 antagonists (e.g. thioperamide, clobenpropit, ciproxifan and GT-2331) in rodent models including the five choice task, object recognition, elevated plus maze, acquisition of novel task and passive avoidance (Giovanni et al., (1999), Behav. Brain Res. 104, 147-155). These data suggest that novel H3 antagonists and/or inverse agonists such as the current series could be useful for the treatment of cognitive impairments in neurological diseases such as Alzheimer's disease and related neurodegenerative disorders.
The present invention provides, in a first aspect, a compound of formula (I) or a pharmaceutically acceptable salt thereof:
wherein:
R1 represents aryl, heteroaryl, -aryl-X—C3-7 cycloalkyl, -heteroaryl-X—C3-7 cycloalkyl, -aryl-X-aryl, -aryl-X-heteroaryl, -aryl-X-heterocyclyl, -heteroaryl-X-heteroaryl, -heteroaryl-X-aryl or -heteroaryl-X-heterocyclyl;
wherein said aryl, heteroaryl and heterocyclyl groups of R1 may be optionally substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, hydroxy, cyano, nitro, oxo, haloC1-6 alkyl, haloC1-6 alkoxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylthio, C1-6 alkoxyC1-6 alkyl, C3-7 cycloalkylC1-6 alkoxy, —COC1-6 alkyl, —CO-haloC1-6 alkyl, —COC1-6 alkyl-cyano, C1-6 alkoxycarbonyl, C1-6 alkylsulfonyl, C1-6 alkylsulfinyl, C1-6 alkylsulfonyloxy, C1-6 alkylsulfonylC1-6 alkyl, C1-6 alkylsulfonamidoC1-6 alkyl, C1-6 alkylamidoC1-6 alkyl, aryl, arylsulfonyl, arylsulfonyloxy, aryloxy, arylsulfonamido, arylcarboxamido, aroyl, or a group —NR15R16, —CONR15R16, —NR15COR16—C(R15)═NOR16—NR15SO2R16 or —SO2NR15R16, wherein R15 and R16 independently represent hydrogen or C1-6 alkyl or together form a heterocyclic ring;
X represents a bond, O, CO, SO2, OCH2 or CH2O;
each R2 and R4 independently represents C1-4 alkyl;
R3 represents C2-6 alkyl, C3-6 alkenyl, C3-6 alkynyl, C3-6 cycloalkyl, C5-6 cycloalkenyl or —C1-4alkyl-C3-6 cycloalkyl;
wherein said C3-6 cycloalkyl groups of R3 may be optionally substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, C1-4 alkyl or trifluoromethyl groups;
m and n independently represent 0, 1 or 2;
p represents 1 or 2;
or a solvate thereof.
In one aspect, the invention provides compounds of formula (I) wherein:
R1 represents aryl, heteroaryl, -aryl-X—C3-7 cycloalkyl, -heteroaryl-X—C3-7 cycloalkyl, -aryl-X-aryl, -aryl-X-heteroaryl, -aryl-X-heterocyclyl, -heteroaryl-X-heteroaryl, -heteroaryl-X-aryl or -heteroaryl-X-heterocyclyl;
wherein said aryl, heteroaryl and heterocyclyl groups of R1 may be optionally substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, hydroxy, cyano, nitro, oxo, haloC1-6 alkyl, haloC1-6 alkoxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylthio, C1-6 alkoxyC1-6 alkyl, C3-7 cycloalkylC1-6 alkoxy, —COC1-6 alkyl, —COC1-6 alkyl-halogen, —COC1-6 alkyl-cyano, C1-6 alkoxycarbonyl, C1-6 alkylsulfonyl, C1-6 alkylsulfinyl, C1-6 alkylsulfonyloxy, C1-6 alkylsulfonylC1-6 alkyl, C1-6 alkylsulfonamidoC1-6 alkyl, C1-6 alkylamidoC1-6 alkyl, aryl, arylsulfonyl, arylsulfonyloxy, aryloxy, arylsulfonamido, arylcarboxamido, aroyl, or a group —NR15R16, —CONR15R16, —NR15COR16—C(R15)═NOR16—NR15SO2R16 or —SO2NR15R16, wherein R15 and R16 independently represent hydrogen or C1-6 alkyl or together form a heterocyclic ring;
X represents a bond, O, CO, SO2, OCH2 or CH2O;
each R2 and R4 independently represents C1-4 alkyl;
R3 represents C2-6 alkyl, C3-6 alkenyl, C3-6 alkynyl, C3-6 cycloalkyl, C5-6 cycloalkenyl or —C1-4alkyl-C3-6 cycloalkyl;
wherein said C3-6 cycloalkyl groups of R3 may be optionally substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, C1-4 alkyl or trifluoromethyl groups;
m and n independently represent 0, 1 or 2;
p represents 1 or 2;
or a solvate thereof.
In another aspect, the aryl, heteroaryl and heterocyclyl groups of R1 may be optionally substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, hydroxy, cyano, nitro, oxo, haloC1-6 alkyl, haloC1-6 alkoxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylthio, C1-6 alkoxyC1-6 alkyl, C3-7 cycloalkylC1-6 alkoxy, —COC1-6 alkyl, —COC1-6 alkyl-halogen, —COC1-6 alkyl-cyano, C1-6 alkoxycarbonyl, C1-6 alkylsulfonyl, C1-6 alkylsulfinyl, C1-6 alkylsulfonyloxy, C1-6 alkylsulfonylC1-6 alkyl, C1-6 alkylsulfonamidoC1-6 alkyl, C1-6 alkylamidoC1-6 alkyl, phenyl, phenylsulfonyl, phenylsulfonyloxy, phenoxy, phenylsulfonamido, phenylcarboxamido, phenylcarbonyl, or a group —NR15R16, —CONR15R16, —NR15COR16, —C(R15)═NOR16, —NR15SO2R16 or —SO2NR15R16, wherein R15 and R16 independently represent hydrogen or C1-6 alkyl or together form a heterocyclic ring.
In a further aspect, in which R1 is pyridin-4-yl or pyrimidin-4-yl optionally substituted by one or two substituents selected from the group consisting of amino, halogen, cyano, C1-6 alkyl or C1-6 alkoxy group, R3 is other than —C1-4alkyl-C5-6 cycloalkyl.
In another aspect in which R1 represents heteroaryl, the heteroaryl group is other than a 1,4-dihydro-quinolin-7-yl group or a 1,4-dihydro-1,8-naphthyridin-7-yl group.
In yet another aspect in which R1 represents heteroaryl, heteroaryl-X-aryl, heteroaryl-X-heteroaryl, heteroaryl-X-heterocyclyl or heteroaryl-X—C3-7 cycloalkyl, and wherein R1 is further substituted, the heteroaryl group directly attached to the nitrogen atom of the pyrrolidine ring is other than a quinoxalinyl group that is substituted by a carboxyamide group at the 5-position and further optionally substituted by a C1-6 alkyl, C1-6 alkoxy, haloC1-6 alkyl, or halogen atom.
A specific set of compounds of formula (I) which may be mentioned are those wherein R1 represents heteroaryl, -heteroaryl-X—C3-7 cycloalkyl, -heteroaryl-X-heteroaryl, -heteroaryl-X-aryl or -heteroaryl-X-heterocyclyl optionally substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, hydroxy, cyano, nitro, haloC1-6 alkyl, haloC1-6 alkoxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylthio, C1-6 alkoxyC1-6 alkyl, C3-7 cycloalkylC1-6 alkoxy, —COC1-6 alkyl, —COC1-6 alkyl-halogen, —COC1-6 alkyl-cyano, C1-6 alkoxycarbonyl, C1-6 alkylsulfonyl, C1-6 alkylsulfinyl, C1-6 alkylsulfonyloxy, C1-6 alkylsulfonylC1-6 alkyl, C1-6 alkylsulfonamidoC1-6 alkyl, C1-6 alkylamidoC1-6 alkyl, aryl, arylsulfonyl, arylsulfonyloxy, aryloxy, arylsulfonamido, arylcarboxamido, aroyl, or a group —NR15R16, —CONR15R16, —NR15COR16, —C(R15)═NOR16, —NR15SO2R16 or —SO2NR15R16, wherein R15 and R16 independently represent hydrogen or C1-6 alkyl or together form a heterocyclic ring.
A more particular set of compounds of formula (I) which may be mentioned are those wherein R1 represents heteroaryl, -heteroaryl-X—C3-7 cycloalkyl, -heteroaryl-X-heteroaryl, -heteroaryl-X-aryl or -heteroaryl-X-heterocyclyl optionally substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, hydroxy, cyano, nitro, haloC1-6 alkyl, haloC1-6 alkoxy, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylthio, C1-6 alkoxyC1-6 alkyl, C3-7 cycloalkylC1-6 alkoxy, —COC1-6 alkyl, —COC1-6 alkyl-halogen, —COC1-6 alkyl-cyano, C1-6 alkoxycarbonyl, C1-6 alkylsulfonyl, C1-6 alkylsulfinyl, C1-6 alkylsulfonyloxy, C1-6 alkylsulfonylC1-6 alkyl, C1-6 alkylsulfonamidoC1-6 alkyl, C1-6 alkylamidoC1-6 alkyl, phenyl, phenylsulfonyl, phenylsulfonyloxy, phenyloxy, phenylsulfonamido, phenylcarboxamido, phenylcarbonyl, or a group —NR15R16, —CONR15R16, —NR15COR16, —C(R15)═NOR16, —NR15SO2R16 or —SO2NR15R16, wherein R16 and R16 independently represent hydrogen or C1-6 alkyl or together form a heterocyclic ring.
The term ‘C1-6 alkyl’ as used herein as a group or a part of the group refers to a linear or branched saturated hydrocarbon group containing from 1 to 6 carbon atoms. Examples of such groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert butyl, n-pentyl, isopentyl, neopentyl or hexyl and the like.
The term ‘C2-6 alkenyl’ as used herein refers to a linear or branched hydrocarbon group containing one or more carbon-carbon double bonds and having from 2 to 6 carbon atoms. Examples of such groups include ethenyl, propenyl, butenyl, pentenyl or hexenyl and the like.
The term ‘C1-6 alkoxy’ as used herein refers to an —O—C1-6 alkyl group wherein C1-6 alkyl is as defined herein. Examples of such groups include methoxy, ethoxy, propoxy, butoxy, pentoxy or hexoxy and the like.
The term ‘C3-8 cycloalkyl’ as used herein refers to a saturated monocyclic hydrocarbon ring of 3 to 8 carbon atoms. Examples of such groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl and the like.
The term ‘halogen’ as used herein refers to a fluorine, chlorine, bromine or iodine atom.
The term ‘haloC1-6 alkyl’ as used herein refers to a C1-6 alkyl group as defined herein wherein at least one hydrogen atom is replaced with halogen. Examples of such groups include fluoroethyl, trifluoromethyl or trifluoroethyl and the like.
The term ‘halo C1-6 alkoxy’ as used herein refers to a C1-6 alkoxy group as herein defined wherein at least one hydrogen atom is replaced with halogen. Examples of such groups include difluoromethoxy or trifluoromethoxy and the like.
The term ‘aryl’ as used herein refers to a C6-12 monocyclic or bicyclic hydrocarbon ring wherein at least one ring is aromatic. More particularly, the term ‘aryl’ refers to a C6-10 monocyclic or bicyclic hydrocarbon ring wherein at least one ring is aromatic. Examples of such groups include phenyl, naphthyl or tetrahydronaphthalenyl and the like.
The term ‘aryloxy’ as used herein refers to an —O-aryl group wherein aryl is as defined herein. Examples of such groups include phenoxy and the like.
The term ‘heteroaryl’ as used herein refers to a 5-6 membered monocyclic aromatic or a fused 8-10 membered bicyclic aromatic ring, which monocyclic or bicyclic aromatic ring contains 1 to 4 heteroatoms selected from oxygen, nitrogen and sulphur. Examples of such monocyclic aromatic rings include thienyl, furyl, furazanyl, pyrrolyl, triazolyl, tetrazolyl, imidazolyl, oxazolyl, thiazolyl, oxadiazolyl, isothiazolyl, isoxazolyl, thiadiazolyl, pyranyl, pyrazolyl, pyrimidyl, pyridazinyl, pyrazinyl, pyridyl, triazinyl, tetrazinyl and the like. Examples of such fused aromatic rings include quinolinyl, isoquinolinyl, quinazolinyl, quinoxalinyl, pteridinyl, cinnolinyl, phthalazinyl, naphthyridinyl, indolyl, isoindolyl, azaindolyl, indolizinyl, indazolyl, purinyl, pyrrolopyridinyl, furopyridinyl, benzofuranyl, isobenzofuranyl, benzothienyl, benzoimidazolyl, benzoxazolyl, benzoisoxazolyl, benzothiazolyl, benzoisothiazolyl, benzoxadiazolyl, benzothiadiazolyl and the like. In one aspect, the term ‘heteroaryl’ refers to a 5-6 membered monocyclic aromatic ring.
The term ‘heterocyclyl’ refers to a 4-7 membered monocyclic ring or a fused or bridged 8-12 membered bicyclic ring which may be saturated or partially unsaturated, which monocyclic or bicyclic ring contains 1 to 4 heteroatoms selected from oxygen, nitrogen or sulphur. Examples of such monocyclic rings include pyrrolidinyl, azetidinyl, pyrazolidinyl, oxazolidinyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, hydantoinyl, valerolactamyl, oxiranyl, oxetanyl, dioxolanyl, dioxanyl, oxathiolanyl, oxathianyl, dithianyl, dihydrofuranyl, tetrahydrofuranyl, dihydropyranyl, tetrahydropyranyl, tetrahydropyridinyl, tetrahydropyrimidinyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, diazepanyl, azepanyl and the like. Examples of such bicyclic rings include indolinyl, isoindolinyl, benzopyranyl, quinuclidinyl, 2,3,4,5-tetrahydro-1H-3-benzazepine, tetrahydroisoquinolinyl and the like.
In one embodiment, R1 represents:
In one aspect, the aryl, heteroaryl or heterocyclic groups of R1 may optionally be substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, cyano, oxo, haloC1-6 alkyl, C1-6 alkyl, C1-6 alkoxy, —COC1-6 alkyl, —COC1-6 alkyl-halogen, C1-6 alkoxycarbonyl, phenyl, phenoyl, or a group —CONR15R16, —NR16COR16 or —C(R15)═NOR16, wherein R15 and R16 independently represent hydrogen or C1-6 alkyl or together form a heterocyclic ring.
More particularly, the aryl, heteroaryl or heterocyclic groups of R1 may optionally be substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, cyano, haloC1-6 alkyl, C1-6 alkyl, C1-6 alkoxy, —COC1-6 alkyl, —COC1-6 alkyl-halogen, C1-6 alkoxycarbonyl, phenyl, phenoyl, or a group —CONR15R16, —NR15COR16 or —C(R15)═NOR16, wherein R15 and R16 independently represent hydrogen or C1-6 alkyl or together form a heterocyclic ring.
Even more particularly, the aryl, heteroaryl or heterocyclic groups of R1 may optionally be substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, cyano, oxo, haloC1-6 alkyl, C1-6 alkyl or —COC1-6 alkyl. Most particularly, the aryl, heteroaryl or heterocyclic groups of R1 may optionally be substituted by one or more (e.g. 1, 2 or 3) substituents which may be the same or different, and which are selected from the group consisting of halogen, cyano, haloC1-6 alkyl, C1-6 alkyl or —COC1-6 alkyl.
In one embodiment in which R1 represents -aryl-X-heteroaryl; -aryl-X-heterocyclyl or -heteroaryl-X-heteroaryl and the aryl or heteroaryl linked to the nitrogen atom of the pyrrolidine group is a 6 membered ring, the bond to X is in the para position relative to the attachment to the linkage to the nitrogen atom of the pyrrolidine group.
In one embodiment in which R1 represents -aryl or -heteroaryl, wherein the aryl and heteroaryl groups are six membered rings that are substituted by one substitutent, the substituent is in the para position relative to the attachment to X.
In a more particular embodiment, R1 represents:
In an even more particular embodiment, R1 represents
Most particularly, R1 represents
In a further embodiment, X represents CO or a bond. In a more particular embodiment, X represents a bond.
In yet another embodiment, m represents 0.
In one embodiment, n represents 0, 1 or 2. In a more particular embodiment, n represents 0 or 1, especially 0.
In embodiments in which R2 is present, R2 may represent methyl.
In one embodiment, p represents 1 or 2. In a more particular embodiment, p represents 1.
In another embodiment, R3 represents C2-6 alkyl (e.g. isopropyl or isopentyl), C3-6 cycloalkyl (e.g. cyclobutyl) or —C1-4alkyl-C3-6 cycloalkyl (e.g. —CH2-cyclopropyl).
In a more particular embodiment, R3 represents isopropyl, cyclobutyl or —CH2-cyclopropyl. Most particularly, R3 represents isopropyl or cyclobutyl.
Compounds of formula (I) may exist as stereoisomers. The 3 position of the pyrrolidine ring is a chiral centre and may exist in R and S forms. In addition, where the pyrrolidine and piperazine rings are substituted by R2 and R4 (i.e. when m and n do not represent 0), the substituted carbon atoms are also chiral centres.
In one embodiment, the stereochemistry of the carbon atom in the pyrrolidine group that is attached to the carbonyl group has the S configuration.
In embodiments in which R2 represents methyl, said R2 group may be attached to the carbon atom adjacent to the N—R3 group. When R2 represents methyl, the stereochemistry of R2 may have the S configuration. In one embodiment where R2 represents methyl and is attached to the carbon atom adjacent to the N—R3 group, the stereochemistry of R2 has the S configuration.
In one aspect of the invention, the invention provides a compound of formula (I) wherein:
R1 represents aryl, aryl-X-heteroaryl, heteroaryl or heteroaryl-X-heteroaryl;
X represents a bond;
m represents 0;
n represents 0 or 1;
p represents 1 or 2;
R2 represents methyl and is attached to the carbon atom adjacent to the N—R3 group;
R3 represents C2-6 alkyl, C3-6 cycloalkyl or —C1-4alkyl-C3-6 cycloalkyl;
wherein said aryl or heteroaryl groups of R1 may be optionally substituted by one or more (e.g. 1, 2 or 3) substitutents which may be the same or different and which are selected from the group consisting of halogen, cyano, oxo, C1-6 alkyl, haloC1-6 alkyl or —COC1-6 alkyl groups;
or a pharmaceutically acceptable salt or solvate thereof.
In a more particular aspect, R1 may be optionally substituted by one or more (e.g. 1, 2 or 3) substitutents which may be the same or different and which are selected from the group consisting of halogen, cyano, C1-6 alkyl, haloC1-6 alkyl or —COC1-6 alkyl groups.
In a further aspect, in which R1 is pyridin-4-yl or pyrimidin-4-yl optionally substituted by one or two substituents selected from the group consisting of halogen, cyano or C1-6 alkyl, R3 does not represent —C1-4alkyl-C5-6 cycloalkyl.
Compounds according to the invention include examples E1-E60 as shown below, or a pharmaceutically acceptable salt thereof.
In a more particular aspect, compounds according to the invention include:
In a most particular aspect, compounds according to the invention include:
Because of their potential use in medicine, the salts of the compounds of formula (I) are preferably pharmaceutically acceptable.
A pharmaceutically acceptable acid addition salt can be formed by reaction of a compound of formula (I) with a suitable inorganic or organic acid (such as hydrobromic, hydrochloric, sulfuric, nitric, phosphoric, succinic, maleic, formic, acetic, propionic, fumaric, citric, tartaric, lactic, benzoic, salicylic, glutamaic, aspartic, p-toluenesulfonic, benzenesulfonic, methanesulfonic, ethanesulfonic, naphthalenesulfonic such as 2-naphthalenesulfonic, or hexanoic acid), optionally in a suitable solvent such as an organic solvent, to give the salt which is usually isolated for example by crystallisation and filtration. A pharmaceutically acceptable acid addition salt of a compound of formula (I) can comprise or be for example a hydrobromide, hydrochloride, sulfate, nitrate, phosphate, succinate, maleate, formate, acetate, propionate, fumarate, citrate, tartrate, lactate, benzoate, salicylate, glutamate, aspartate, p-toluenesulfonate, benzenesulfonate, methanesulfonate, ethanesulfonate, naphthalenesulfonate (e.g. 2-naphthalenesulfonate) or hexanoate salt.
Free base compounds may be converted into the corresponding hydrochloride salts by treatment in methanol with a solution of hydrogen chloride in diethyl ether followed by evaporation of solvents.
The invention includes within its scope all possible stoichiometric and non-stoichiometric forms of the salts of the compounds of formula (I) including hydrates and solvates.
Compounds of formula (I) are capable of existing in stereoisomeric forms. It will be understood that the invention encompasses all geometric and optical isomers of these compounds and the mixtures thereof including racemates. The different stereoisomeric forms may be separated one from the other by methods known in the art (e.g. separation by chiral HPLC), or any given isomer may be obtained by stereospecific or asymmetric synthesis. The invention also extends to any tautomeric forms and mixtures thereof.
In one aspect, the stereochemistry at the 3 position of the pyrrolidine ring of the compound of formula (I) is in the S configuration. Compounds with this stereochemistry are referred to as compounds of formula (Ia).
The present invention also provides a process for the preparation of a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof, which process comprises:
(a) reacting a compound of formula (II)
or an optionally activated or protected derivative thereof, wherein R2, R4, m, n and p are as defined above and R3a is as defined for R3 above or a group convertible to R3, with a compound of formula R1-L1, wherein R1 is as defined above and L1 represents a suitable leaving group, such as a halogen atom (e.g. fluorine, chlorine, bromine or iodine) or triflate group, followed by a deprotection reaction as necessary; or
(b) reacting a compound of formula (III)
wherein R1, R4 and m are as defined above and L2 represents OH or a suitable leaving group, such as a halogen atom (e.g. chlorine), with a compound of formula (IV)
wherein R2, n and p are as defined above R3a is as defined for R3 above or a group convertible to R3; or
(c) deprotecting a compound of formula (I) or converting groups which are protected; or
(d) interconversion from one compound of formula (I) to another.
Process (a) typically comprises the use of a suitable base, such as potassium carbonate in a suitable solvent such as dimethylsulfoxide or N,N-dimethylformamide at elevated temperature. Alternatively process (a) may be carried out with a suitable catalyst in the presence of a suitable base such as sodium t-butoxide or potassium phosphate in a solvent such as o-xylene, dioxane, toluene or dimethoxyethane under an inert atmosphere, optionally at an elevated temperature. Suitable catalysts include tris(dibenzylideneacetone)dipalladium(0) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)biphenyl, bis(dibenzylideneacetone)palladium and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)biphenyl, acetato(2′-di-t-butylphosphin-1,1′-biphenyl-2-yl)palladium II or tris(dibenzylideneacetone)dipalladium(0) and 2-(dicyclohexylphosphino)biphenyl.
An R3a group convertible to R3 may for example be a protecting group such as tert-butoxycarbonyl which may be removed under acidic conditions, e.g. trifluoroacetic acid or HCl or a benzyloxycarbonyl or benzyl group which may be removed by hydrogenolysis, to give a compound where R3a represents hydrogen. Subsequent conversion to a compound where R3a represents R3 may be carried out by reductive amination with a compound of formula R3′═O (where R3′ may be convertible to a group R3) in the presence of sodium triacetoxyborohydride or alkylation with a compound of formula R3-L3 where L3 is a leaving group such as bromine or iodine.
Process (b) typically comprises activation of the compound of formula (III) wherein L2 represents OH with a coupling reagent such as 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) in the presence of 1-hydroxybenzotriazole (HOBT) or 1-hydroxy-7-azabenzotriazole (HOAT) in a suitable solvent such as dichloromethane or dimethylformamide and optionally in the presence of a suitable base, followed by reaction with the compound of formula (IV) or a salt of this compound.
When L2 represents a halogen (e.g. chlorine) atom, process (b) takes place in the presence of a suitable base such as triethylamine or a solid supported base such as diethylaminomethylpolystyrene in a suitable solvent such as dichloromethane. Compounds of formula (III) in which L2 represents a halogen atom may be prepared from compounds of formula (III) wherein L2 represents OH by treatment with a suitable halogenating agent (e.g. thionyl chloride or oxalyl chloride).
In process (c), examples of protecting groups and the means for their removal can be found in T. W. Greene ‘Protective Groups in Organic Synthesis’ (J. Wiley and Sons, 1991). Suitable amine protecting groups include sulphonyl (e.g. tosyl), acyl (e.g. acetyl, 2′,2′,2′-trichloroethoxycarbonyl, benzyloxycarbonyl or tert-butoxycarbonyl) and arylalkyl (e.g. benzyl), which may be removed by hydrolysis (e.g. using an acid such as hydrochloric acid) or reductively (e.g. hydrogenolysis of a benzyl group or reductive removal of a 2′,2′,2′-trichloroethoxycarbonyl group using zinc in acetic acid) as appropriate. Other suitable amine protecting groups include trifluoroacetyl (—COCF3) which may be removed by base catalysed hydrolysis or a solid phase resin bound benzyl group, such as a Merrifield resin bound 2,6-dimethoxybenzyl group (Ellman linker), which may be removed by acid catalysed hydrolysis, for example with trifluoroacetic acid.
Process (d) may be performed using conventional interconversion procedures such as epimerisation, oxidation, reduction, alkylation, nucleophilic or electrophilic aromatic substitution, ester hydrolysis or amide bond formation. Examples of transition metal mediated coupling reactions useful as interconversion procedures include the following: Palladium catalysed coupling reactions between organic electrophiles, such as aryl halides, and organometallic reagents, for example boronic acids (Suzuki cross-coupling reactions); Palladium catalysed amination and amidation reactions between organic electrophiles, such as aryl halides, and nucleophiles, such as amines and amides; Copper catalysed amidation reactions between organic electrophiles (such as aryl halides) and nucleophiles such as amides; and Copper mediated coupling reactions between phenols and boronic acids.
Compounds of formula (II) may be prepared in accordance with the following procedure:
wherein R2, R4, m, n and p are as defined above, R3a is as defined for R3 above or a group convertible to R3, L3 represents OH or a suitable leaving group such as a halogen atom (e.g. chlorine), and P1 represents a suitable protecting group such as t-butoxycarbonyl.
When L3 represents OH, step (i) typically comprises the use of suitable coupling conditions e.g. 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) in the presence of 1-hydroxybenzotriazole (HOBT) or 1-hydroxy-7-azabenzotriazole (HOAT), optionally in the presence of a suitable base and in a suitable solvent such as dichloromethane or dimethylformamide.
When L3 represents a suitable leaving group such as a halogen atom (e.g. chlorine), step (i) typically comprises the use of a suitable base such as triethylamine or a solid supported base such as diethylaminomethylpolystyrene in a suitable solvent such as dichloromethane.
Step (ii) typically comprises a suitable deprotection reaction using standard conditions such as those described above for process (c). Where P1 is a tert butoxycarbonyl group this may involve a suitable acid such as HCl or trifluoroacetic acid
Compounds of formula (III) wherein L2 represents OH, may be prepared in accordance with the following procedure:
wherein R1, R4 and m are as defined above, L4 represents a suitable leaving group such as a halogen atom or triflate group and P2 represents a suitable protecting group such as methoxy, ethoxy, t-butoxy or benzyloxy.
Step (i) is typically carried out in a suitable solvent such as N,N-dimethylformamide or dimethylsulfoxide in the presence of a base such as potassium carbonate at elevated temperature. Alternatively step (i) may be carried out with a suitable catalyst in the presence of a suitable base such as sodium t-butoxide or potassium phosphate in a solvent such as o-xylene, dioxane, toluene or dimethoxyethane under an inert atmosphere optionally at an elevated temperature. Suitable catalysts include tris(dibenzylideneacetone)dipalladium(0) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)biphenyl, bis(dibenzylideneacetone)palladium and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)biphenyl, acetato(2′-di-t-butylphosphino-1,1′-biphenyl-2-yl)palladium II, or tris(dibenzylideneacetone)dipalladium(0) and 2-(dicyclohexylphosphino)biphenyl.
Step (ii) typically comprises a suitable deprotection reaction using standard conditions such as those described above for process (c). Where P2 is an alkoxy group such as ethoxy this may involve suitable acid or base catalysed hydrolysis e.g. using aqueous hydrochloric acid or a base such as sodium hydroxide or lithium hydroxide.
Compounds of formula (III) wherein L2 represents a suitable leaving group, such as a halogen atom (e.g. chlorine) may be prepared by treating a compound of formula (III)a with thionyl chloride or oxalyl chloride.
Stereoisomers of optionally substituted pyrrolidine-3-carboxylic acid in which the stereochemistry at the 3 position of the pyrrolidine ring is in either the R or S configurations may be prepared in accordance with the procedure set forth below wherein R4 and m are as described above, P3 is a protecting group, such as benzyloxycarbonyl or tert-butoxycarbonyl, OX represents a leaving group such as a mesylate, tosylate or triflate group, cNu represents a carbon nucleophile that can be converted to a carboxylic acid, and L5 and L6 represent suitable leaving groups such as a halogen atom (e.g. a chlorine atom). This scheme shows the production of the S enantiomer, however, it will be appreciated that the R enantiomer may be produced by an analogous process.
Step (i) is typically carried out in a suitable solvent such as dichloromethane in the presence of a suitable base such as triethylamine at a suitable temperature such as 0° C. to room temperature.
Step (ii) is typically carried out in a suitable solvent such as dichloromethane in the presence of a suitable base such as triethylamine at a suitable temperature such as 0° C. to room temperature.
Step (iii) is typically carried out by reaction with a carbon nucleophile that can be converted to a carboxylic acid, such as a cyanide salt (eg. KCN), in a suitable solvent such as DMSO at a suitable temperature such as 90° C.
Step (iv) refers to the situation where P3 is not hydrolysed in acidic conditions. This step typically comprises the use of an acid such as concentrated hydrochloric acid at a suitable temperature such as reflux.
Step (v) typically comprises a suitable deprotection reaction using standard conditions such as those described above for process (c).
Step (vi) refers to the situation where P3 can be hydrolysed, for example, where P3 is a acid labile urethane protecting group such as benzyloxycarbonyl or tert-butoxycarbonyl. This step typically comprises the use of an acid such as concentrated hydrochloric acid at a suitable temperature such as reflux. The acid hydrolyses both the urethane protecting group and the cyano/nitrile group.
Compounds of formula (IX) wherein m represents 0 are commercially available (e.g. from Lancaster). Compounds of formula (IX) wherein m represents 1 or 2 may be prepared by following procedures described or analogous to those described in the literature. For example, (3R,5R)-5-methyl-3-pyrrolidinol could be prepared following procedures described in WO2005/060665 (Scheme 10, 10-5).
Compounds of formula (XIV) may be used to prepare stereoisomers of the compounds of formula (V) and compounds of formula (VII) wherein the stereochemistry at the 3 position of the pyrrolidine ring is in the S or R configuration. This enables stereoisomers of the compounds of formula (I) to be prepared, wherein the stereochemistry at the 3 position of the pyrrolidine ring is in either the R or S configurations.
Stereoisomers of the compounds of formula (XIV) in which the stereochemistry at the 3 position of the pyrrolidine ring is in the S or R configurations may be used to prepare stereoisomers of the compound of formula (V) in which the stereochemistry at the 3 position of the pyrrolidine ring is in either the R or S configurations, by reaction with a compound of formula P1-L7, wherein P1 is as described above and L7 is a suitable leaving group such as a halogen atom. This reaction typically takes place in a suitable solvent such as dichloromethane in the presence of a suitable base such as triethylamine at a suitable temperature such as 0° C. to room temperature. Where P1 is tert-butoxycarbonyl, the reaction is typically carried out using di-tertbutyldicarbonate in a suitable solvent such as aqueous acetone at a suitable temperature, such as 0° C.
Stereoisomers of the compounds of formula (XIV) in which the sterochemistry at the 3 position of the pyrrolidine ring is in the S or R configurations may be used to prepare stereoisomers of the compound of formula (VII) in which the stereochemistry at the 3 position of the pyrrolidine ring is in either the R or S configurations, by reaction with a compound of formula P2—H, wherein P2 is as described above. This reaction typically takes place in acidic conditions at a suitable temperature, such as room temperature.
The process described above is one of a number of possible methods for producing stereoisomers of the compounds of formula (I) in which the stereochemistry at the 3 position of the pyrrolidine ring is in either the R or S configurations. The methods envisaged all share a step of reacting a pyrrolidine derivative with a carbon nucleophile that can be converted to a carboxylic acid as set forth below:
wherein R4 and m are as defined above, wherein L7 represents a leaving group, such as a halogen atom or a leaving group defined by OX above, wherein Y represents a protecting group or R1 as defined above, and wherein cNu represents a carbon nucleophile that can be converted to a carboxylic acid.
The step indicated above is typically carried out by reaction with a carbon nucleophile that can be converted to a carboxylic acid, such as a cyanide salt (e.g. KCN), in a suitable solvent such as DMSO at a suitable temperature such as 90° C.
This scheme shows the production of the S enantiomer, however, it will be appreciated that the R enantiomer may be produced by an analogous process.
Compounds of formula (IV), (V), (VII), (IX) and R1-L4 are either known in the literature or can be prepared by analogous methods.
Compounds of formula (I) and their pharmaceutically acceptable salts have affinity for and are antagonists and/or inverse agonists of the histamine H3 receptor and are believed to be of potential use in the treatment of neurological diseases including Alzheimer's disease, dementia (including Lewy body dementia and vascular dementia), age-related memory dysfunction, mild cognitive impairment, cognitive deficit, epilepsy, pain of neuropathic origin including neuralgias, neuritis and back pain, and inflammatory pain including osteoarthritis, rheumatoid arthritis, acute inflammatory pain and back pain, migraine, Parkinson's disease, multiple sclerosis, stroke and sleep disorders (including narcolepsy and sleep deficits associated with Parkinson's disease); psychiatric disorders including schizophrenia (particularly cognitive deficit of schizophrenia), attention deficit hypereactivity disorder, depression, anxiety and addiction; and other diseases including obesity and gastro-intestinal disorders.
It will also be appreciated that compounds of formula (I) are expected to be selective for the histamine H3 receptor over other histamine receptor subtypes, such as the histamine H1 receptor. Generally, compounds of the invention may be at least 10 fold selective for H3 over H1, such as at least 100 fold selective.
Thus the invention also provides a compound of formula (I) or a pharmaceutically acceptable salt thereof, for use as a therapeutic substance in the treatment or prophylaxis of the above disorders, in particular cognitive impairments in diseases such as Alzheimer's disease and related neurodegenerative disorders.
The invention further provides a method of treatment or prophylaxis of the above disorders, in mammals including humans, which comprises administering to the sufferer a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof.
In another aspect, the invention provides the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for use in the treatment of the above disorders.
When used in therapy, the compounds of formula (I) are usually formulated in a standard pharmaceutical composition. Such compositions can be prepared using standard procedures.
Thus, the present invention further provides a pharmaceutical composition for use in the treatment of the above disorders which comprises the compound of formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.
The present invention further provides a pharmaceutical composition which comprises the compound of formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.
Compounds of formula (I) may be used in combination with other therapeutic agents, for example medicaments claimed to be useful as either disease modifying or symptomatic treatments of Alzheimer's disease. Suitable examples of such other therapeutic agents may be agents known to modify cholinergic transmission such as 5-HT6 antagonists, M1 muscarinic agonists, M2 muscarinic antagonists or acetylcholinesterase inhibitors. When the compounds are used in combination with other therapeutic agents, the compounds may be administered either sequentially or simultaneously by any convenient route. The invention thus provides, in a further aspect, a combination comprising a compound of formula (I) or a pharmaceutically acceptable derivative thereof together with a further therapeutic agent or agents.
The combinations referred to above may conveniently be presented for use in the form of a pharmaceutical formulation and thus pharmaceutical formulations comprising a combination as defined above together with a pharmaceutically acceptable carrier or excipient comprise a further aspect of the invention. The individual components of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations.
When a compound of formula (I) or a pharmaceutically acceptable derivative thereof is used in combination with a second therapeutic agent active against the same disease state the dose of each compound may differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art.
A pharmaceutical composition of the invention, which may be prepared by admixture, suitably at ambient temperature and atmospheric pressure, is usually adapted for oral, parenteral or rectal administration and, as such, may be in the form of tablets, capsules, oral liquid preparations, powders, granules, lozenges, reconstitutable powders, injectable or infusible solutions or suspensions or suppositories. Orally administrable compositions are generally preferred.
Tablets and capsules for oral administration may be in unit dose form, and may contain conventional excipients, such as binding agents, fillers, tabletting lubricants, disintegrants and acceptable wetting agents. The tablets may be coated according to methods well known in normal pharmaceutical practice.
Oral liquid preparations may be in the form of, for example, aqueous or oily suspension, solutions, emulsions, syrups or elixirs, or may be in the form of a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, emulsifying agents, non-aqueous vehicles (which may include edible oils), preservatives, and, if desired, conventional flavourings or colorants.
For parenteral administration, fluid unit dosage forms are prepared utilising a compound of the invention or pharmaceutically acceptable salt thereof and a sterile vehicle. The compound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions, the compound can be dissolved for injection and filter sterilised before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, preservatives and buffering agents are dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. Parenteral suspensions are prepared in substantially the same manner, except that the compound is suspended in the vehicle instead of being dissolved, and sterilisation cannot be accomplished by filtration. The compound can be sterilised by exposure to ethylene oxide before suspension in a sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound. The composition may contain from 0.1% to 99% by weight, preferably from 10 to 60% by weight, of the active material, depending on the method of administration. The dose of the compound used in the treatment of the aforementioned disorders will vary in the usual way with the seriousness of the disorders, the weight of the sufferer, and other similar factors. However, as a general guide suitable unit doses may be 0.05 to 1000 mg, more suitably 0.1 to 200 mg and even more suitably 1.0 to 200 mg, and such unit doses may be administered more than once a day, for example two or three a day. Such therapy may extend for a number of weeks or months.
The following Descriptions and Examples illustrate the preparation of compounds of the invention. An Emrys™ Optimizer microwave reactor was employed for reactions carried out with microwave heating. Where indicated, Varian Mega BE (10 g) SCX columns or Isolute Flash SCX-2 (20 g) columns were used for the work-up of reactions. Crude mixtures were applied to the column, non-polar materials were washed off with methanol, and the desired amines were eluted with ammonia in methanol. In addition, where indicated, Mass Directed Auto-Purification or MDAP was carried out using a purification system supplied by Waters. The columns used were Waters Atlantis (19 mm×100 mm or 30 mm×100 mm). The solvent systems used comprised solvent A (water+0.1% formic acid) and solvent B (acetonitrile+0.1% formic acid) with gradients within the range 5-99% solvent B in solvent A.
Description 1
Method A
Benzyl chloroformate (27.4 ml) was added to a stirred solution of (R)-3-pyrrolidinol (obtainable from Lancaster 19499; 16 g) and triethylamine (26.7 ml) in dry DCM (250 ml) at 0° C. over 10 min under an inert atmosphere. The mixture was allowed to warm to room temperature and stirred for a further 2 h. The solvent was evaporated and the residue partitioned between EtOAc (250 ml) and aqueous 0.5M HCl (80 ml). The organic layer was separated, washed with saturated sodium hydrogen carbonate solution (50 ml) and brine (50 ml), dried (MgSO4) and evaporated to give the title compound (D1) as a pale orange oil (39 g). LCMS electrospray (+ve) 244 (MNa+)
Method B
Benzyl chloroformate (90 ml of 95% purity) was dissolved in DCM (100 ml) and added dropwise to a stirred, ice-bath cooled, solution of (R)-3-pyrrolidinol (obtainable commercially from Lancaster; 50 g) and triethylamine (84 ml) in DCM (700 ml) over 40 min under argon. The mixture was allowed to warm to room temperature and stirred for a further 3 h. The mixture was transferred to a separating funnel and washed with 0.5M aqueous HCl (100 ml) and saturated sodium hydrogen carbonate solution (100 ml). The organic layer was dried (MgSO4) and evaporated to afford the title compound (D1) as a pale orange oil (126.1 g). LCMS electrospray (+ve) 244 (MNa+)
Description 2
Method A
Methanesulfonyl chloride (15 ml) was added to a stirred solution of benzyl (3R)-3-hydroxy-1-pyrrolidinecarboxylate (may be prepared as described in Description 1, method A) (39 g) and triethylamine (30 ml) in DCM (400 ml) at 0° C. over 10 min under an inert atmosphere. The reaction was allowed to warm to room temperature and stirred for a further 0.5 h. The reaction mixture was then washed with saturated sodium hydrogen carbonate solution (80 ml), brine (2×50 ml), dried (MgSO4) and evaporated to afford the title compound (D2) as a pale orange oil (50.7 g). LCMS electrospray (+ve) 322 (MNa+)
Method B
Each of two 63 g batches of benzyl (3R)-3-hydroxy-1-pyrrolidinecarboxylate (may be prepared as described in Description 1, method B) was separately dissolved in DCM (500 ml) and triethylamine (47.5 ml). The resultant solutions were stirred and cooled in an ice-bath under argon. Methanesulfonyl chloride (24.5 ml) was added dropwise to each solution over 20 min. Each reaction was allowed to warm to room temperature and stirred for a further 1.5 h. Both reaction mixtures were then combined in a separating funnel and washed with saturated sodium hydrogen carbonate solution (250 ml) and brine (2×200 ml). The organic layer was dried (Na2SO4) and evaporated in vacuo to afford the title compound (D2) as a thick orange oil (169 g). LCMS electrospray (+ve) 322 (MNa+)
Description 3
Method A
Potassium cyanide (21.8 g) was added to a stirred solution of benzyl (3R)-3-[(methylsulfonyl)oxy]-1-pyrrolidinecarboxylate (may be prepared as described in Description 2, method A) (50 g) in DMSO (300 ml) and the mixture stirred at 90° C. for 4 h. The DMSO was evaporated to a minimum and the residue dissolved in EtOAc (500 ml), washed with water (2×50 ml), dried (MgSO4) and evaporated. The residue was purified by flash chromatography [silica gel, EtOAc:hexane 1:1] and the pure fractions evaporated to give the title compound (D3) as a pale yellow oil (17.5 g). LCMS electrospray (+ve) 253 (MNa+)
Method B
Each of two 84.5 g batches of benzyl (3R)-3-[(methylsulfonyl)oxy]-1-pyrrolidinecarboxylate (may be prepared as described in Description 2, method B) was separately dissolved in DMSO (650 ml) and finely ground potassium cyanide (46 g) was added to each solution. Both reactions were stirred at 90° C. for 9 h. The crude reaction mixtures were then combined and the DMSO evaporated in vacuo. Brine (600 ml) was added to the residue and the mixture extracted with EtOAc (5×400 ml). The combined organic extracts were then dried (Na2SO4) and evaporated in vacuo. The residue was purified by chromatography on silica (Flash 75 system), eluting with a gradient (0-50% EtOAc in hexane) and the pure fractions evaporated to afford the title compound (D3) as a pale yellow oil (89.4 g). LCMS electrospray (+ve) 253 (MNa+)
Description 4
Method A
Benzyl (3S)-3-cyano-1-pyrrolidinecarboxylate (may be prepared as described in Description 3, method A) (17.5 g) was heated at reflux in a mixture of conc. hydrochloric acid (200 ml) and glacial acetic acid (40 ml) for 4 h. The reaction mixture was evaporated in vacuo and re-evaporated from toluene (2×100 ml). The residue was dissolved in water (50 ml) and acetone (30 ml), then sodium carbonate (8.5 g) and di-tert-butyldicarbonate (19.9 g) were added sequentially. The mixture was stirred at room temperature for 16 h before evaporation of the acetone in vacuo. The remaining aqueous solution was washed with diethyl ether (2×20 ml), cooled in an ice-bath, acidified to pH 3-4 with 2M hydrochloric acid and extracted with EtOAc (3×100 ml). The combined organic extracts were dried (MgSO4) and evaporated to afford the title compound (D4) as a colourless solid (10.1 g). LCMS electrospray (−ve) 214 (M-H). 1H NMR (DMSO-d6) δ 1.39 (9H, s), 1.91-2.05 (2H, m), 2.99-3.06 (1H, m), 3.22-3.41 (4H, m), 12.48 (1H, bs)
Method B
Benzyl (3S)-3-cyano-1-pyrrolidinecarboxylate (may be prepared as described in Description 3, method B) (88.5 g) was heated at reflux in a mixture of conc. hydrochloric acid (450 ml) and glacial acetic acid (100 ml) for 3 h. The reaction mixture was evaporated to dryness in vacuo and the residue dissolved in water (200 ml) and acetone (120 ml). The solution was cooled in an ice-bath, sodium carbonate (42.8 g) was then added portionwise over 10 min followed by di-tert-butyldicarbonate (101 g) and the resultant mixture stirred at room temperature for 16 h. Acetone was evaporated from the mixture in vacuo and the remaining aqueous solution was washed with diethyl ether (2×50 ml). The aqueous layer was then cooled in an ice-bath, the pH was adjusted to pH 3-4 with 2M hydrochloric acid (keeping internal temperature below 10° C.) and extracted with EtOAc (4×400 ml). The combined organic extracts were dried (Na2SO4) and evaporated. The residue was dissolved in a minimum volume of hot EtOAc and allowed to crystallise overnight. The residue was filtered and dried to afford the title compound (D4) as an off-white crystalline solid (54.6 g). A further 4.8 g was obtained by evaporating the mother liquors and recrystallising the residue from hot EtOAc. LCMS electrospray (−ve) 214 (M-H). 1H NMR (DMSO-d6) δ 1.39 (9H, s), 1.91-2.08 (2H, m), 2.99-3.07 (1H, m), 3.18-3.44 (4H, m), 12.48 (1H, bs)
Description 5
Method A
EDC (3.21 g), HOBT (1.13 g) and 1-(1-methylethyl)piperazine (obtainable from Chess 1214; 1.07 g) were added sequentially to a stirred solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4, method A) (1.8 g) in DMF (20 ml) and the reaction mixture stirred at room temperature under argon for 3 h. The DMF was removed by evaporation and the residue partitioned between EtOAc/saturated sodium hydrogen carbonate solution (120:20 ml). The organic layer was dried (Na2SO4), evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The clean fractions were evaporated to afford the title compound (D5) as a pale yellow oil (2.37 g). LCMS electrospray (+ve) 348 (MNa+)
Method B
EDCl (35.6 g) and HOBT (12.6 g) were added sequentially to a stirred solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4, method B) (20 g) in DMF (200 ml). 1-(1-Methylethyl)piperazine (obtainable commercially from Chess; 13.3 ml) was then added and the reaction mixture stirred at room temperature under argon for 3 h. The DMF was evaporated to a minimum and the residue partitioned between EtOAc/water (500:150 ml). The layers were separated and the aqueous layer extracted with EtOAc (3×400 ml). The combined extracts were washed with brine (40 ml), dried (Na2SO4) and evaporated to afford the title compound (D5) as a pale orange oil (27.1 g). LCMS electrospray (+ve) 348 (MNa+)
Method C
EDCl (17.8 g), HOBT (6.28 g) and 1-(1-Methylethyl)piperazine (obtainable commercially from Chess; 5.96 g, 6.65 ml) were added to a solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4, method B) (10 g) in DMF (100 ml). The reaction mixture was stirred at room temperature under argon for 3 h. The DMF was evaporated, followed by a co-evaporation from ethyl acetate. The residue was partitioned between ethyl acetate (300 ml) and water (60 ml). The aqueous layer was re-extracted with ethyl acetate (240 ml) and combined with the original ethyl acetate layer. The organic extracts were dried (MgSO4), filtered and evaporated to dryness to afford the title compound (D5) as a brown oil (10.8 g).
LCMS electrospray (+ve) 326 (MH+)
Description 6
Method A
4M HCl in dioxane (15 ml) was added to a solution of tert-Butyl (3S)-3-{[4-(1-methylethyl)-1-piperazinyl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 5, method A) (2.37 g) in MeOH (15 ml) and the mixture stirred for 3 h under an argon atmosphere. The reaction mixture was then evaporated to dryness and the residue basified with saturated potassium carbonate solution (10 ml) and extracted with DCM (3×50 ml). The combined organic extracts were dried (Na2SO4) and evaporated to afford the title compound (D6) as a pale yellow thick oil (1.46 g). LCMS electrospray (+ve) 226 (MH+). 1H NMR (CDCl3) δ1.05 (6H, d, J=6.4 Hz), 1.92-2.03 (3H, m), 2.47-2.52 (4H, m), 2.67-2.93 (3H, m), 3.04-3.13 (2H, m), 3.18-3.22 (1H, m), 3.52-3.55 (2H, m), 3.61-3.64 (2H, m).
Method B
4M HCl in dioxane (60 ml) was added to a solution of tert-Butyl (3S)-3-{[4-(1-methylethyl)-1-piperazinyl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 5, method B) (27 g) in MeOH (60 ml) and the mixture stirred for 4 h under an argon atmosphere. The solvent was removed in vacuo and the residue dissolved in saturated potassium carbonate solution (100 ml) and extracted with DCM (4×400 ml). The combined organic extracts were dried (Na2SO4) and evaporated to afford the title compound (D6) as a pale orange oil (12.92 g). LCMS electrospray (+ve) 226 (MH+). 1H NMR (CDCl3) δ 1.05 (6H, d, J=6.4 Hz), 1.91-2.04 (3H, m), 2.47-2.53 (4H, m), 2.67-2.93 (3H, m), 3.04-3.13 (2H, m), 3.18-3.22 (1H, m), 3.52-3.55 (2H, m), 3.61-3.64 (2H, m).
Method C
4M HCl in dioxane (50 ml) was added to a solution of tert-Butyl (3S)-3-{[4-(1-methylethyl)-1-piperazinyl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 5, method C) (10.8 g) in MeOH (50 ml) and the mixture stirred for 16 h under an argon atmosphere. The reaction mixture was evaporated to dryness and the residue dissolved in methanol and loaded onto an SCX cartridge, eluting with methanol, followed by 2M ammonia/methanol. The appropriate fractions were combined and evaporated to dryness, to afford the title compound (D6) as an orange/brown oil (4.66 g).
LCMS electrospray (+ve) 226 (MH+)
Description 7
1-tert-Butoxycarbonyl-piperazine (5.6 g) and cyclobutanone (2.10 g) were dissolved in DCM (100 ml) and the reaction mixture was stirred at room temperature for 30 min. Sodium triacetoxyborohydride (6.37 g) was added portion-wise over 10 min. The mixture was then stirred overnight. The reaction mixture was washed with 1N NaOH (70 ml) and the DCM layer was dried (MgSO4) and evaporated to give the title compound as an oil (6.5 g) 1H NMR δ [DMSO-d6]: 1.39 (9H, s), 1.68-1.87 (4H, m), 1.9-2.01 (2H, m), 2.15-2.2 (3H, m), 2.5 (1H, m), 2.6-2.78 (1H, m), 3.18-3.3 (4H, m).
Description 8
1-tert-Butoxycarbonyl-4-cyclobutyl-piperazine (may be prepared as described in Description 7) (6.1 g) was dissolved in dry MeOH (70 ml) followed by the addition of 4N HCl in dioxane (20 ml). The reaction mixture was stirred at room temperature under argon for 4 hours. The solvent was evaporated and the precipitate was filtered off and dried in a vacuum oven to give the title compound (D8) as a white solid (4.05 g). 1H NMR (DMSO): 1.63-1.80 (2H, m), 2.12-2.19 (2H, m), 2.34-2.39 (2H, m), 3.08 (2H, m), 3.43-3.56 (6H, m), 3.71 (1H, m), 9.70 (2H, bs) and 12.40 (1H, bs).
Description 9
EDC (2.79 g), HOBT (0.956 g) were added to a solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4) (1.522 g) in DMF (50 ml) and the reaction was stirred for 10 minutes. Triethylamine (2.88 ml) was then syringed into the reaction mixture and this was followed by addition of 1-cyclobutyl-piperazine dihydrochloride (may be prepared as described in Description 8) (1.5 g) dissolved in DMF (25 ml). The reaction mixture was stirred at room temperature for 3 h. 50 ml water was added to the reaction mixture and it was then washed with 3 portions of EtOAc (30 ml). The organic layer was washed twice with brine (30 ml) and seven times with saturated NaHCO3 (30 ml). Following this, the organic component was dried over MgSO4 and filtered. The filtrate was evaporated of solvent to give the title compound (D9) as a light brown solid (2.81 g). LCMS electrospray (+ve) 360 (MNa+)
Description 10
4M HCl in dioxane (5 ml) was added to a solution of tert-Butyl (3S)-3-[(4-cyclobutyl-1-piperazinyl)carbonyl]-1-pyrrolidinecarboxylate (may be prepared as described in Description 9) (2.81 g) in MeOH (75 ml) and the mixture was left to stand overnight. The solvent was then evaporated to give a brown oil. The residue was dissolved in MeOH and loaded onto an SCX column. The column was washed with MeOH and eluted with 2M NH3/MeOH. Ammoniacal fractions were combined and the solvent was evaporated to afford the title compound (D10) as a brown oil (0.8 g). LCMS electrospray (+ve) 238 (MH+). 1H NMR (CDCl3) δ 1.61-2.10 (9H, m), 2.27-2.32 (4H, m), 2.68-3.20 (6H, m), 3.52-3.55 (2H, m), 3.62-3.65 (2H, m).
Description 11
EDC (3.21 g), HOBT (1.13 g) and 1-(1-ethylpropyl)-piperazine (1.31 g) were added sequentially to a stirred solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4) (1.8 g) in DMF (20 ml) and the reaction mixture stirred at room temperature under argon for 3 h. The DMF was removed by evaporation and the residue partitioned between EtOAc/saturated sodium hydrogen carbonate solution (120:20 ml). The organic layer was dried (Na2SO4), evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The clean fractions were evaporated to afford the title compound (D11) as a pale yellow oil (2.80 g). LCMS electrospray (+ve) 376 (MNa+)
Description 12
4M HCl in dioxane (15 ml) was added to a solution of tert-Butyl (3S)-3-{[4-(1-ethylpropyl)-1-piperazinyl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 11) (2.80 g) in MeOH (15 ml) and the mixture stirred for 3 h under an argon atmosphere. The reaction mixture was then evaporated to dryness and the residue basified with saturated potassium carbonate solution (10 ml) and extracted with DCM (3×50 ml). The combined organic extracts were dried (Na2SO4) and evaporated to afford the title compound (D12) as a pale yellow thick oil (2.0 g). LCMS electrospray (+ve) 254 (MH+). 1H NMR (CDCl3) δ 0.90 (6H, t, J=7.2 Hz), 1.22-1.36 (2H, m), 1.37-1.51 (2H, m), 1.88-2.02 (2H, m), 2.14-2.21 (1H, m), 2.46-2.52 (4H, m), 2.78-3.22 (6H, m), 3.47-3.50 (2H, m), 3.56-3.59 (2H, m).
Description 13
(Bromomethyl)cyclopropane (3.9 ml) was added to a stirred mixture of potassium carbonate (6.9 g) and tert-butyl hexahydro-1H-1,4-diazepine-1-carboxylate (Aldrich 51, 138-2; 5.0 g) in acetonitrile (70 ml) and the mixture heated at reflux for 5 h. The solvent was evaporated and the residue partitioned between EtOAc (100 ml) and water (30 ml). The organic layer was dried, evaporated and the residue purified by chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The pure fractions were evaporated to afford the title compound (D13) as a pale yellow solid (3.25 g). LCMS electrospray (+ve) 255 (MH+).
Description 14
4M HCl in dioxane (10 ml) was added to a solution of the tert-Butyl 4-(cyclopropylmethyl)-hexahydro-1H-1,4-diazepine-1-carboxylate (may be prepared as described in Description 13) (3.25 g) in MeOH (10 ml) and the solution was stirred for 2.5 h at room temperature. The solvent was evaporated to give the title compound (D14) as a beige solid (2.8 g). LCMS electrospray (+ve) 155 (MH+).
Description 15
EDC (2.67 g), HOBT (0.94 g) and 1-(cyclopropylmethyl)-hexahydro-1H-1,4-diazepine dihydrochloride (may be prepared as described in Description 14) (1.6 g) were added sequentially to a stirred solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4) (1.5 g) in DMF (20 ml). Diisopropylethylamine (2.67 ml) was added and the reaction mixture stirred at room temperature under argon for 3 h. The DMF was removed by evaporation and the residue partitioned between EtOAc/saturated sodium hydrogen carbonate solution (100:10 ml). The organic layer was dried (Na2SO4) and evaporated to afford the title compound (D15) as a pale yellow oil (1.96 g). LCMS electrospray (+ve) 352 (MH+)
Description 16
4M HCl in dioxane (10 ml) was added to a solution of tert-butyl (3S)-3-{[4-(cyclopropylmethyl)-hexahydro-1H-1,4-diazepin-1-yl]carbonyl}pyrrolidine-1-carboxylate (may be prepared as described in Description 15) (1.96 g) in MeOH (10 ml) and the mixture stirred overnight under an argon atmosphere. The reaction mixture was then evaporated to dryness and the residue dissolved in MeOH, loaded onto SCX (20 g) and the cartridge eluted with MeOH followed by 2MNH3/MeOH (50 ml). The ammoniacal fractions were evaporated to afford the title compound (D16) as a pale orange oil (0.795 g). LCMS electrospray (+ve) 252 (MH+). 1H NMR (CDCl3) δ 0.0-0.1 (2H, m), 0.39-0.48 (2H, m), 0.70-0.81 (1H, m), 1.74-1.96 (5H, m), 2.29 (2H, t, J=6.4 Hz), 2.57-2.76 (5H, m), 2.85 (1H, dd, J=11.2, 8.4 Hz), 2.97-3.13 (3H, m), 3.47-3.59 (4H, m).
Description 17
(S)-2-methylpiperazine (10 g) was dissolved in dry DCM (200 ml). Triethylamine (14.5 ml) was added and the reaction was cooled to 0° C. Benzyl chloroformate (18.66 g) in dry DCM (30 ml) was added dropwise to the reaction mixture, which was then stirred at room temperature for 3 h. The DCM layer was then washed with saturated bicarbonate and water, dried (MgSO4) and filtered and stripped to give an oil. The oil was absorbed onto silica and then purified by chromatography on a silica column (Flash, size E column) eluting with a gradient of MeOH/NH4OH in DCM (the MeOH/NH4OH component being comprised of methanol containing 10% 0.88 ammonia solution). The product was eluted with 6% of the NH4OH/MeOH component to give the title compound (D17) as an oil (10.18 g). LCMS electrospray (+ve) 235 (MH+).
Description 18
Potassium carbonate (8.8 g) was added to a solution of (3S)-1-benzyloxycarbonyl-3-methylpiperazine (may be prepared as described in Description 17) (7.5 g) in CH3CN (100 ml), followed by isopropyl iodide (10.0 ml) and the mixture was heated at reflux overnight. The reaction mixture was then allowed to cool to room temperature and the potassium carbonate was filtered off. The filtrate was stripped to give an oil which was partitioned between EtOAc and water. The EtOAc layer was dried (MgSO4), filtered and stripped to give the title compound (D18) as an oil (8.39 g). LCMS electrospray (+ve) 277 (MH+).
Description 19
(2S)-1-(1-methylethyl)-4-(benzyloxycarbonyl)-2-methylpiperazine (may be prepared as described in Description 18) (8.39 g) was dissolved in EtOH (150 ml) and treated with 10% Pd/C paste (4 heaped spatulas) and stirred under atmospheric hydrogen conditions overnight. The catalyst was filtered off and the solvent was removed by evaporation. 1N ethereal HCl (100 ml) was added and the reaction mixture was stripped, filtered and dried in a dessicator to give the title compound (D18) (4.5 g). LCMS electrospray (+ve) 143 (MH+).
Description 20
EDC (1.73 g), HOBT (0.609 g) and (2S)-1-(1-methylethyl)-2-methylpiperazine dihydrochloride (may be prepared as described in Description 19) (0.978 g) and diisopropylethylamine (1.73 ml) were added sequentially to a solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4) (0.97 g) in DMF (10 ml) and the reaction mixture was stirred at room temperature under argon for 3 h. Most of the DMF was removed by evaporation and the residue partitioned between EtOAc/saturated sodium hydrogen carbonate solution (150:20 ml). The layers were separated and the organic layer was washed with water (5 ml), dried (MgSO4), evaporated and the residue purified by chromatography on a silica column (FM(II) system), eluting with 0-10% 2M NH3/MeOH in DCM. The clean fractions were evaporated to afford the title compound (D20) as a pale yellow oil (0.86 g). LCMS electrospray (+ve) 362 (MNa+)
Description 21
4M HCl in dioxane (10 ml) was added to a solution of tert-butyl (3S)-3-{[(3S)-4-(1-methylethyl)-3-methyl-1-piperazinyl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 20) (0.86 g) in MeOH (5 ml) and the mixture stirred overnight under an argon atmosphere. The solvent was removed in vacuo and the residue was dissolved in MeOH (20 ml), loaded onto a 20 g SCX cartridge and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were evaporated and dried to afford the title compound (D21) as a pale yellow oil (0.58 g). LCMS electrospray (+ve) 240 (MH+). 1H NMR (CDCl3) δ 0.84 (3H, d, J=6.4 Hz), 1.06 (3H, t, J=7.2), 1.09 (3H, d, J=6.4 Hz), 1.90-2.04 (4H, m), 2.19-2.29 (1H, m), 2.50-3.29 (9H, m), 3.62-3.83 (1H, m), 4.17-4.29 (1H, m).
Description 22
4-Bromobenzamide (51.48 g) was heated in N,N-dimethylacetamide dimethylacetal (165 ml) at 120° C. for 2 h. The solution was allowed to cool overnight and the product crystallised as pale yellow needles, which were collected by filtration, washed on the filter with diethyl ether and dried overnight at 40° C. in vacuo to give the title compound (D22) (57.84 g). 1H NMR δ [DMSO-d6]: 2.26 (3H, s), 3.13 (3H, s), 3.14 (3H, s), 7.61 (2H, d, J=8.6 Hz), 7.94 (2H, d, J=8.6 Hz).
Description 23
4-Bromo-N-[(1-(dimethylamino)ethylidene]benzamide (may be prepared as described in Description 22) (57.8 g) was treated with a solution of hydroxylamine hydrochloride (19.6 g) in 1M NaOH solution (350 ml). Dioxane (350 ml) and glacial acetic acid (450 ml) were added, and the resulting solution was stirred at 25° C. for 30 min and then at 90° C. for 3 h. After cooling overnight, the crystalline product (colourless needles) was collected by filtration, washed with dilute aqueous acetic acid and water and dried at 50° C. in vacuo to give the title compound (D23). Concentration of the filtrate yielded a second crop of product, spectroscopically identical to the first, which was collected and dried as before (46.1 g total). 1H NMR δ [CDCl3]: 2.48 (3H, s), 7.67 (2H, d, J=8.4 Hz), 7.98 (2H, d, J=8.4 Hz); (MH)+=239, 241.
Description 24
tert-Butyl hexahydro-1H-1,4-diazepine-1-carboxylate (Aldrich 51, 138-2; 10.0 g) was dissolved in DCM (300 ml). Cyclobutanone (7.5 ml) was added and the reaction was left to stir for 5 min. Sodium triacetoxyborohydride (21.1 g) was then added and the reaction was stirred at room temperature for 16 h. The reaction mixture was washed with saturated potassium carbonate solution (2×200 ml). The organic layer was dried (MgSO4) and evaporated to give the title compound (D24) as a clear oil (11.3 g).
Description 25
tert-Butyl 4-(cyclobutyl)-hexahydro-1H-1,4-diazepine-1-carboxylate (may be prepared as described in Description 24) (11.3 g) was dissolved in methanol (200 ml) and 4N HCl in dioxan (100 ml) was added. The reaction was stirred at room temperature for 3 h and then co-evaporated from toluene (3×50 ml) to give the title compound (D25) as a white solid (9.8 g). 1H NMR δ (DMSO-d6): 11.95 (1H, s), 9.55 (1H, s), 9.64 (1H, s), 3.78-3.08 (9H, m), 2.51-2.07 (6H, m), 1.80-1.51 (2H, m).
Description 26
(Bromomethyl)cyclopropane (2.86 ml) was added to a stirred mixture of potassium carbonate (7.4 g) and tert-butyl 1-piperazinecarboxylate (5.0 g) in acetonitrile (70 ml) and the mixture heated at reflux for 5 h. The mixture was cooled, filtered and the solvent evaporated. The residue was dissolved in MeOH (20 ml), added to 4M HCl in dioxane (20 ml) and the solution stirred for 2.5 h at room temperature. The precipitate was filtered off and dried to give the title compound (D26) as a white solid (4.4 g). LCMS electrospray (+ve) 141 (MH+).
Description 27
EDC (2.67 g), HOBT (0.94 g) and 1-(cyclopropylmethyl)piperazine dihydrochloride (1.5 g) (may be prepared as described in Description 26) were added sequentially to a stirred solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 4) (1.5 g) in DMF (20 ml). Diisopropylethylamine (2.67 ml) was added and the reaction mixture stirred at room temperature under argon for 3 h. The DMF was removed by evaporation and the residue partitioned between EtOAc/saturated sodium hydrogen carbonate solution (100:10 ml). The organic layer was dried (MgSO4) and evaporated to afford the title compound (D27) as a pale yellow oil (1.83 g). LCMS electrospray (+ve) 338 (MH+)
Description 28
4M HCl in dioxane (20 ml) was added to a solution of tert-butyl (3S)-3-{[4-(cyclopropylmethyl)-1-piperazinyl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 27) (1.83 g) in MeOH (10 ml) and the mixture stirred for 16 h under an argon atmosphere and then evaporated to dryness. The residue was dissolved in MeOH (50 ml), loaded onto a 20 g SCX cartridge and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were evaporated to afford the title compound (D28) as a pale yellow thick oil (1.16 g). LCMS electrospray (+ve) 238 (MH+). 1H NMR (CDCl3) δ: 0.0-0.1 (2H, m), 0.39-0.48 (2H, m), 0.70-0.81 (1H, m), 1.80-1.92 (2H, m), 2.10-2.20 (3H, m), 2.36-2.42 (4H, m), 2.69-3.11 (5H, m), 3.43-3.57 (4H, m).
Description 29
1-(tert-Butoxycarbonyl)-3-pyrrolidinecarboxylic acid (6.6 g) in dry DMF (150 ml) was treated with EDC (12.17 g) and HOBT (4.17 g). The reaction mixture was then stirred at room temperature for 20 min. 1-(Cyclobutyl)hexahydro-1H-1,4-diazepine dihydrochloride (may be prepared as described in Description 25) (6.66 g) was added to the stirred solution followed by triethylamine (12.8 ml) and the reaction mixture was stirred at room temperature overnight. The mixture was then poured into water (500 ml) and extracted with EtOAc (2×100 ml). The combined organic layers were washed with water (5×100 ml), dried (MgSO4) and concentrated to give a brown oil (8.8 g). LCMS electrospray (+ve) 352 (MH+).
Description 30
Method A
(R,S)-1-tert-Butyl 3-[(4-cyclobutylhexahydro-1H-1,4-diazepin-1-yl)carbonyl]-1-pyrrolidinecarboxylate (may be prepared as described in Description 29) (1.7 g) in dry MeOH (30 ml) was treated with 4N dioxane HCl (10 ml) and stirred at room temperature overnight. The reaction mixture was concentrated to dryness to give a brown oil (1.7 g) which was dissolved in water (5 ml) and treated with excess solid K2CO3 to liberate the free base which was extracted into EtOAc. The organic extracts were dried (MgSO4) and concentrated to give the title compound (D30) as an oily residue (1.08 g). LCMS electrospray (+ve) 252 (MH+).
Method B
A solution of (R,S)-1-tert-Butyl 3-[(4-cyclobutylhexahydro-1H-1,4-diazepin-1-yl)carbonyl]-1-pyrrolidinecarboxylate (may be prepared as described in Description 29) (8.8 g) in MeOH (200 ml), was treated with 4N dioxane HCl (40 ml) and then stirred at room temperature overnight. The solvent was evaporated to give a brown gum which was dissolved in MeOH (60 ml) and divided into 3 aliquots. Each aliquot (20 ml) was poured on to a 50 g SCX column which was washed with MeOH (50 ml) and then eluted with 2N MeOH ammonia solution (40 ml). The ammonia solutions were combined and evaporated to dryness to the give the title compound (D30) (5.5 g) LCMS electrospray (+ve) (252) (MH+).
Description 31
4-Bromo-3-fluorobenzoic acid (10.09 g) was heated at reflux in thionyl chloride (100 ml) for 4 h and then allowed to cool. The mixture was evaporated in vacuo and the residue re-evaporated with DCM (2×) to give the acid chloride as a light brown oil. This was added dropwise to vigorously stirred, ice-cooled concentrated aqueous ammonia (100 ml) and the precipitated product was collected by filtration, washed on the filter with water and dried at 40° C. in vacuo to give 4-bromo-3-fluorobenzamide as a white solid (9.13 g). This material and N,N-dimethylacetamide dimethyl acetal (27 ml) were heated together at 120° C. for 2 h. The reaction was allowed to cool to room temperature and the liquid evaporated in vacuo to give a brown gum which was partitioned between saturated aqueous sodium hydrogen carbonate and EtOAc. The organic extract was washed with water and brine, dried and evaporated to give the acylamidine intermediate as a gum which solidified in vacuo, overnight (12.3 g). This intermediate was treated with a solution of hydroxylamine hydrochloride (4.16 g) in 1M aqueous NaOH (74.2 ml), dioxane (75 ml) and glacial acetic acid (95 ml). The reaction mixture was first stirred at room temperature for 30 min then heated at 90° C. for 3 h. On cooling a first crop of crystals was filtered off and dried in vacuo at 50° C. to give the title compound (D31) (5.5 g). The filtrate afforded a second crop of crystals (2.1 g). LCMS electrospray (+ve) 257 and 259 (MH+).
Description 32
4-Bromo-2-fluorobenzoic acid (5.27 g) was heated at reflux in thionyl chloride (50 ml) for 4 h and then allowed to cool. The mixture was evaporated in vacuo and the residue re-evaporated with DCM (2×) to give the acid chloride as a light brown oil. This was added dropwise to vigorously stirred, ice-cooled concentrated aqueous ammonia (50 ml) and when addition was complete the mixture was stirred for 5 min and then extracted (3×) with EtOAc. The combined organic extracts were washed with water and brine, dried (Na2SO4) and evaporated to give 4-bromo-2-fluorobenzamide as a white solid (4.72 g). This material and N,N-dimethylacetamide dimethyl acetal (17 ml) were heated together at 120° C. for 2 h. The reaction was allowed to cool to rt and the liquid evaporated in vacuo to give a brown gum which was partitioned between saturated aqueous sodium hydrogen carbonate and EtOAc. The organic extract was washed with water and brine, dried (Na2SO4) and evaporated to a gum. This was purified by chromatography (silica gel, eluant hexane/EtOAc) to give the acylamidine intermediate as a gum which solidified in vacuo (4.15 g). Hydroxylamine hydrochloride (1.32 g) in 1N NaOH solution (23.5 ml) was added, followed by dioxane (23.5 ml) then AcOH (30 ml). The reaction mixture was stirred at rt for 30 min then heated at 90° C. for 3 h. The reaction was allowed to cool to rt and poured into water. The pH was adjusted to ˜9 by addition of solid NaHCO3 and the precipitated product was collected by filtration, washed on the filter with water and dried at 40° C. in vacuo to give the title compound (D32) as a greyish-brown solid (2.82 g). LCMS electrospray (+ve) 257 and 259 (MH+).
Description 33
Method A
tert-Butyl hexahydro-1H-1,4-diazepine-1-carboxylate (obtainable from Aldrich 51, 138-2; 10.0 g) was dissolved in DCM (200 ml). Acetone (7.33 ml) was added and the reaction was left to stir for 5 min. Sodium triacetoxyborohydride (21.0 g) was then added and the reaction was stirred at room temperature for 16 h. The reaction mixture was washed with saturated potassium carbonate solution (2×200 ml). The organic layer was dried (magnesium sulphate) and evaporated to give the title compound (D33) as a clear oil (11.0 g).
Method B
tert-Butyl hexahydro-1H-1,4-diazepine-1-carboxylate (obtainable from Aldrich 51, 138-2; 25.06 g) was dissolved in acetonitrile (250 ml). Anhydrous potassium carbonate (34.5 g) and 2-iodopropane (63 g, 37 ml) were added and the mixture was heated at reflux for 18 h. The cooled mixture was filtered and the solids were washed with acetonitrile. The combined filtrates were evaporated and the residual oil was dissolved in diethyl ether, washed with water, sodium thiosulphate solution and brine, dried (Na2SO4) and evaporated to give the title compound (D33) as a light brown oil (29.8 g).
Description 34
1-tert-Butyl 4-(1-methylethyl)-hexahydro-1H-1,4-diazepine-1-carboxylate (may be prepared as described in Description 33) (11.0 g) was dissolved in methanol (200 ml) and 4N HCl in dioxan (100 ml) was added. The reaction was stirred at room temperature for 2 h and then evaporated to give the title compound (D34) as a white solid (9.6 g). 1H NMR δ (CDCl3): 11.35 (1H, s), 10.22 (1H, s), 9.72 (1H, s), 4.15-3.52 (9H, m), 2.83-2.40 (2H, m), 1.47 (6H, d, J=6.24 Hz).
Description 35
EDC (3.57 g), HOAT (0.1 g) and 1-(1-methylethyl)-hexahydro-1H-1,4-diazepine dihydrochloride (may be prepared as described in Description 34) (2.0 g) were added sequentially to a stirred solution of 1-(tert-butoxycarbonyl)-3-pyrrolidine carboxylic acid (2.0 g) in DMF (80 ml). Diisopropylethylamine (4.06 ml) was added and the reaction mixture stirred at room temperature under argon for 3 h. Saturated sodium hydrogen carbonate solution (100 ml) was added and the mixture extracted with EtOAc (100 ml×3) The combined organic extracts were dried (Na2SO4), evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The pure fractions were evaporated to afford the title compound (D35) as a white solid (1.55 g). LCMS electrospray (+ve) 362 (MNa+)
Description 36
4M HCl in dioxane (10 ml) was added to a solution of (R,S)-tert-butyl 3-{[4-(1-methylethyl)-hexahydro-1H-1,4-diazepin-1-yl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 35) (1.52 g) in MeOH (10 ml) and the mixture stirred for 2 h under an argon atmosphere. The reaction mixture was then evaporated to dryness and the residue basified with saturated potassium carbonate solution (10 ml) and extracted with DCM (3×50 ml). The combined organic extracts were dried (Na2SO4) and evaporated to afford the title compound (D36) as a colourless oil (0.78 g). 1H NMR (CDCl3) δ 0.99 (6H, d, J=6.5 Hz), 1.77-2.05 (4H, m), 2.52-3.12 (11H, m), 3.52-3.62 (4H, m).
Description 37
The title compound (D37) was prepared using an analogous process to that described in Descriptions 1 to 4 from (S)-3-pyrrolidinol (Lancaster 19498). LCMS electrospray (−ve) 214 (M-H). 1H NMR (DMSO-d6) δ 1.39 (9H, s), 1.91-2.05 (2H, m), 2.99-3.06 (1H, m), 3.22-3.41 (4H, m), 12.48 (1H, bs).
Description 38
The title compound (D38) was prepared using an analogous process to that described in Descriptions 5 and 6 from (3R)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 37) and 1-cyclobutylhexahydro-1H-1,4-diazepine dihydrochloride (may be prepared as described in Description 25). LCMS electrospray (+ve) 252 (MH+).
Description 39
Method A
EDC (4.5 g), HOBT (1.6 g) and 1-(1-methylethyl)hexahydro-1H-1,4-diazepine dihydrochloride (may be prepared as described in Description 34) (2.5 g) were added sequentially to a stirred solution of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidine carboxylic acid (may be prepared as described in Description 4) (2.5 g) in DMF (25 ml). Diisopropylethylamine (4.45 ml) was added and the reaction mixture stirred at room temperature under argon for 2 h. The DMF was removed by evaporation and the residue partitioned between EtOAc/saturated sodium hydrogen carbonate solution (100:50 ml). The layers were separated and the aqueous layer extracted with EtOAc (100 ml). The combined organic layers were dried (Na2SO4), evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The clean fractions were evaporated to afford the title compound (D39) as a pale yellow oil (2.2 g). LCMS electrospray (+ve) 362 (MNa+).
Method B
1-(1-Methylethyl)hexahydro-1H-1,4-diazepine dihydrochloride (may be prepared as described in Description 34) (15 g) and diisopropylethylamine (26.8 ml) were added sequentially to a mixture of (3S)-1-(tert-butoxycarbonyl)-3-pyrrolidine carboxylic acid (may be prepared as described in Description 4) (15 g), EDC (26.7 g) and HOBT (9.4 g) in DMF (150 ml) and the reaction stirred for 16 h. The DMF was evaporated and the residue partitioned between EtOAc/saturated sodium hydrogen carbonate solution (500:150 ml). The layers were separated and the aqueous layer extracted with EtOAc (4×350 ml). The combined organic layers were dried (MgSO4) and evaporated to afford the title compound (D39) as an orange oil (21.5 g). LCMS electrospray (+ve) 362 (MNa+).
Description 40
Method A
4M HCl in dioxane (10 ml) was added to a solution of (3S)-1,1-dimethylethyl 3-{[4-(1-methylethyl)hexahydro-1H-1,4-diazepin-1-yl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 39) (2.2 g) in MeOH (10 ml) and the mixture stirred for 2 h under an argon atmosphere. The solvent was evaporated and the residue dissolved in MeOH (30 ml) and divided into 3 aliquots. Each aliquot was loaded onto a 10 g SCX cartridge which was eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were evaporated to afford the title compound (D40) as a pale orange oil (1.33 g). LCMS electrospray (+ve) 240 (MH+).
Method B
4M HCl in dioxane (60 ml) was added to a solution of (3S)-1,1-dimethylethyl 3-{[4-(1-methylethyl)hexahydro-1H-1,4-diazepin-1-yl]carbonyl}-1-pyrrolidinecarboxylate (may be prepared as described in Description 39) (21.5 g) in MeOH (60 ml) and the mixture stirred for 1 h under an argon atmosphere. The solvent was evaporated and the residue partitioned between DCM/saturated potassium carbonate solution (400:100 ml). The layers were separated and the aqueous layer extracted with DCM (5×200 ml). The combined organic layers were dried (MgSO4) and evaporated to afford the title compound (D40) as an orange oil (9.97 g). LCMS electrospray (+ve) 240 (MH+).
Description 41
The title compound was prepared from (3R)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 37) and 1-(1-methylethyl)piperazine (obtainable from Chess 1214) using an analogous process to that described in Descriptions 5 and 6 LCMS electrospray (+ve) 226 (MH+) 1H NMR (CDCl3) δ 1.05 (6H, d, J=6.4 Hz), 1.92-2.03 (3H, m), 2.47-2.52 (4H, m), 2.67-2.93 (3H, m), 3.04-3.13 (2H, m), 3.18-3.22 (1H, m), 3.52-3.55 (2H, m), 3.61-3.64 (2H, m).
Description 42
The title compound was prepared from (3R)-1-(tert-butoxycarbonyl)-3-pyrrolidinecarboxylic acid (may be prepared as described in Description 37) and 1-cyclobutylpiperazine dihydrochloride (may be prepared as described in Description 8) using an analogous process to that described in Descriptions 9 and 10. LCMS electrospray (+ve) 238 (MH+). 1H NMR (CDCl3) δ 1.61-2.10 (9H, m), 2.27-2.32 (4H, m), 2.68-3.20 (6H, m), 3.52-3.55 (2H, m), 3.62-3.65 (2H, m).
Description 43
Method A
4-Bromophenacyl bromide (21.3 g) and acetamide (11.31 g) were heated together in an oil bath at 130° C. under argon. After 2.5 h the reaction mixture was allowed to cool, and partitioned between water and diethyl ether. The organic phase was washed with aqueous NaOH (0.5M), aqueous HCl (0.5M) and brine (100 ml), dried (Na2SO4) and evaporated to give a brown solid which was recrystallised from hexanes and dried in a vacuum oven overnight at 60° C. to give the title compound (D43) as an orange solid (4.1 g). LCMS electrospray (+ve) 238 and 240 (MH+).
Method B
A solution of 4-Bromophenacyl bromide (12.7 g) and acetonitrile (6.8 g) in NMP (40 ml) was heated at reflux for 2.5 h. The reaction was cooled to room temperature, diluted with 300 ml ether, washed with 1M NaOH (50 ml), 2M HCl (50 ml) and brine (50 ml), dried (MgSO4) and evaporated. The residue was recrystallised from hexane to afford an orange solid which was further purified by silica chromatography (FM(II) system, 100 g), eluting with a gradient of 0-35% ethyl acetate in pentane. The clean fractions were evaporated to afford the title compound (D43) as a pale yellow solid (4.1 g). LCMS electrospray (+ve) 238 and 240 (MH+).
Description 44
4-Bromophenylcarbonitrile (10.2 g), hydroxylamine hydrochloride (7.8 g) and triethylamine (11.3 g) were dissolved in EtOH (250 ml) and the reaction mixture was heated at reflux for 3 h, after which it was evaporated to form a white precipitate of the desired amidoxime, which was filtered and washed with water (25 ml). The filtrate was extracted into EtOAc (2×25 ml), and the combined organic extracts were dried (Na2SO4) and evaporated to give a second crop of the title compound (D44) (combined yield=11.1 g). LCMS electrospray (+ve) 215 and 217 (MH+).
Description 45
4-Bromo-N-hydroxy-benzenecarboximidamide (D44) was suspended in acetic anhydride and heated to 100° C. for 4 h, then 120° C. for 3 h. After cooling the reaction mixture was evaporated to give a brown solid. This was partitioned between saturated aqueous sodium hydrogen carbonate and EtOAc. The organic phase was washed with saturated brine, dried (Na2SO4) and evaporated to give a yellow solid. The crude product was purified by column chromatography (silica gel, 10-100% gradient of EtOAc in hexanes) to give the title compound (D45) as a white solid (6.2 g). LCMS electrospray (+ve) 239 and 241 (MH+).
Description 46
Trifluoromethanesulfonic acid (6.6 ml) was added to a flask containing iodobenzene diacetate (12.2 g) and MeCN (200 ml) at rt. After 25 min a solution of 4′-bromoacetophenone (5 g) in MeCN (50 ml) was added and the resultant mixture heated at reflux for 6 h. The reaction was allowed to cool to rt before the solvent was evaporated and the residue partitioned between saturated aqueous sodium hydrogen carbonate (150 ml) and EtOAc (150 ml). The organic phase was washed with saturated brine (150 ml), dried (MgSO4) and evaporated to give an orange solid. The crude product was purified by column chromatography (silica gel, 50% EtOAc in hexanes) to give the title compound (D46) as a pale yellow solid (3.5 g). LCMS electrospray (+ve) 238 and 240 (MH+).
Description 47
A mixture of potassium fluoride (1.77 g) and cuprous iodide (5.79 g) was stirred and heated together using a heat gun under vacuum (˜1 mm) for 20 min. After cooling, dimethylformamide (20 ml) and N-methylpyrrolidinone (20 ml) were added followed by (trifluoromethyl)trimethylsilane (4.1 ml) and 5-bromo-2-iodopyrimidine (6.5 g). The mixture was stirred at rt for 5 h and then the brown solution was poured into 6N ammonia solution. The product was extracted into ethyl acetate and the extracts were washed with saturated aqueous sodium hydrogen carbonate solution and brine and then dried (Na2SO4) and evaporated. Chromatography on silica gel (elution with 20-50% dichloromethane in pentane) gave the title compound (D47) as a white solid (2.4 g). 1H NMR (CDCl3): 8.97 (2H, s).
Description 48
N-Hydroxyethanimidamide (2.58 g) was added to a suspension of NaH (60% dispersion in mineral oil, 1.44 g) in THF (100 ml) and the mixture stirred for 10 min at room temperature followed by 50 min at 50° C. Methyl 3-bromobenzoate (5 g) in THF (100 ml) was added and the mixture heated to reflux for 1 h. The reaction was allowed to cool, poured into water (100 ml) and extracted with EtOAc (2×100 ml). The combined extracts were washed with water (2×20 ml) and brine (20 ml), dried (Na2SO4) and evaporated. The solid residue was recrystallised from hexane to afford the title compound (D48) as white needles (3.08 g). LCMS electrospray (+ve) 239, 241 (MH+) 1H NMR (CDCl3) δ 2.47 (3H, s), 7.41 (1H, t, J=7.6 Hz), 7.72 (1H, m), 8.05 (1H, m) and 8.28 (1H, t, J=1.6 Hz).
Description 49
Propionitrile (7.7 g) and hydroxylamine (50% aq., 4.3 ml) were heated to reflux in EtOH (10 ml) for 18.5 h The reaction mixture was allowed to cool to room temperature before extracting into DCM (25 ml). The organic phase was washed with saturated brine (50 ml), water (25 ml), dried (MgSO4) and evaporated. Analysis of the crude mixture indicated approximately 30% conversion. More hydroxylamine (10 ml) and EtOH (5 ml) were added and the reaction mixture was heated for a further 7 h at 90° C. After cooling and extraction into DCM (25 ml) as before, the organic phase was again washed with saturated brine (50 ml), water (25 ml), dried (MgSO4) and evaporated to give the crude amidoxime as an oil. This was combined with 4-bromobenzoic acid (2.2 g), EDC (2.2 g) and HOBt (1.47 g) in DMF (25 ml), and Hunig's base (7 ml) was added. The reaction mixture was stirred for 4 h. The reaction mixture was then divided into two aliquots which were heated in the microwave reactor at 180° C. for 20 min. each. The two crude mixtures were combined and the DMF evaporated. The crude oxadiazole was purified by flash chromatography [silica gel, 0-20% EtOAc/pentane]. The clean fractions were evaporated to afford the title compound (D49) as a yellow solid (1.5 g). LCMS electrospray (+ve) 253/255 (MH+). 1H NMR (CDCl3) δ 1.39 (3H, t, J=7.6 Hz), 2.83 (2H, q, J=7.6 Hz), 7.65 (2H, d, J=8.0 Hz) and 7.97 (2H, d, J=8.0 Hz).
Description 50
5-Bromo-2-pyridinecarbonitrile (15 g) was heated at reflux in concentrated hydrochloric acid (200 ml) for 6 hours. On cooling in an ice bath the product crystallised out as white needles which were filtered and washed with iced water and dried in an oven under vacuum to give the title compound (D50) (11 g). LCMS electrospray (+ve) 202 and 204 (MH+).
Description 51
5-Bromo-2-pyridinecarboxylic acid (may be prepared as described in Description 50; 4.5 g) and carbonyldiimidazole (3.97 g) in THF (40 ml) were heated at reflux temperature for 90 minutes followed by the addition of acetamide oxime (4.95 g). The reaction mixture was allowed to continue refluxing overnight. After cooling to room temperature the reaction mixture was diluted with EtOAc and washed with water (2×), 2M NaOH (2×), water (2×) followed by brine (2×). The organic layer was dried (MgSO4) and concentrated to give a pale yellow solid which was recrystallised from a mixture of hot ethanol and methanol to give the title compound (D51) as colourless crystals (4.4 g). LCMS electrospray (+ve) 240 and 242 (MH+).
Description 52
2,4-Difluorobenzamide (19.98 g) in N,N-dimethylacetamide dimethylacetal (82 ml) was heated at 120° C. for 2 hours and allowed to cool overnight. The crude reaction mixture was diluted with water (500 ml) and extracted with EtOAc (2×200 ml). The EtOAc layer was dried (MgSO4), filtered and evaporated (stripped) to give an oil. The residue was absorbed onto silica (100 g) and purified by silica chromatography (on a size E column), eluting with a gradient of from 10-100% EtOAc in hexane. The pure fractions were combined and stripped to give the title compound (D52) (24 g). LCMS (+ve) electrospray 227 (MH+).
Description 53
To a stirred mixture of hydroxylamine hydrochloride (9.9 g) and 1N NaOH (140 ml) was added N-[1-(dimethylamino)ethylidene]-2,4-difluorobenzamide (may be prepared as described in Description 52; 23 g), acetic acid (170 ml) and dioxane (140 ml) sequentially. The mixture was stirred for 20 minutes at room temperature and was then heated at reflux for 3 hours. After cooling the crude reaction mixture was poured into water (1 litre) and adjusted to pH9 with NaHCO3. The mixture was filtered and the residue was diluted with EtOAc (1 litre). The EtOAc layer was filtered and the filtrate was evaporated to dryness to give the title compound as a light brown solid (11.2 g). LCMS electrospray (+ve) 197 (MH+).
Description 54
A mixture of 4-methylimidazole (1.642 g), 4-bromoiodobenzene (5.658 g), caesium carbonate (13.70 g), cuprous iodide (0.192 g) and trans-N,N-dimethyl-1,2-cyclohexane diamine (0.579 g) in DMF (10 ml) was added to a Buchi miniclave (25 ml) according to the method of Buchwald et al. (Journal of Organic Chemistry, 2004, 69: 5578). The reactants were added under a gentle stream of argon. The tube was then sealed and the mixture was heated at 110° C. for 18-20 h. After cooling, the reaction mixture was poured into water and extracted (3×) with ethyl acetate. The combined extracts were washed with water (2×) and brine, dried (MgSO4) and evaporated to give a light brown semi-solid. This was filtered through SiO2, eluting with 0-80-100% EtOAc in hexane. The resulting light brown solid was recrystallised from hexane/toluene. The product, which was obtained as light yellow needles (2.086 g), consisted of a mixture of 4-(4-methylimidazol-1-yl)iodobenzene and 4-(4-methylimidazol-1-yl)bromobenzene (D54) as evidenced by the spectroscopic data. 1H NMR δ (CDCl3): 2.30 (3H, s), 6.97 (1H, s), 7.12 (2H, d, J=8.8 Hz, iodo compound), 7.24 (2H, d, J=8.4 Hz, bromo compound), 7.59 (2H, d, J=8.8 Hz, bromo compound), 7.73 (1H, s), 7.78 (2H, d, J=8.8 Hz, iodo compound); LCMS electrospray (+ve) (MH+) 237/239 (Rt=1.47 min., 34%, bromo compound), 285 (Rt=1.57 min., 66%, iodo compound)
Description 55
To an ice-cooled, stirred solution of acetamidoxime (28.0 g; only partially soluble initially) and triethylamine (42.1 g) in THF (950 mL) under an atmosphere of argon, was added a solution of 4-fluorobenzoyl chloride (60.0 g) in THF (350 ml), dropwise, over 56 min. The cooling bath was then removed and the resulting slurry was stirred for 57 min. DBU (115.1 g) was added fairly rapidly from an addition funnel, which was then washed through with THF (50 ml). The stirred mixture was heated at 63° C. for 16 h and then allowed to cool to room temperature. The solid precipitate was filtered off, washed on the filter with THF and discarded. The combined filtrate was concentrated in vacuo and the resulting material was partitioned between 2M HCl (1.0 l) and Et2O (0.51). The layers were separated and the aqueous acidic layer was re-extracted twice with diethyl ether (0.5 l). The combined ethereal extracts were washed sequentially with water (0.5 l), sat. NaHCO3, water (0.51) and brine; dried (MgSO4) and evaporated to give a white solid (51.7 g). This was combined with another batch prepared similarly from identical quantities of the starting materials (crude weight 56 g) and the combined batches were recrystallised from a 50:50 mixture of water/glacial acetic acid (700 ml), affording the title product (D55) as white needles which were collected by filtration, washed on the filter with 50:50 water/glacial acetic acid (220 ml) and dried at 39° C. in vacuo, over KOH pellets for 48 h to give the dry product (D55) (81.65 g) 1H NMR (CDCl3) 2.47 (3H, s), 7.22 (2H, m), 8.13 (2H, m); LCMS electrospray (+ve) 179 (MH+).
Description 56
EDC (0.696 g), HOBT (0.23 g), 1-ethylpiperazine (0.2 g) and triethylamine (0.25 ml) were added sequentially to a stirred solution of (3S)-1-(tert-butoxycarbonyl-3-pyrrolidinecarboxylic acid (0.38 g) (may be prepared as described in Description 4) in DMF (20 ml) and the reaction mixture was stirred at room temperature overnight. The reaction mixture was diluted with water (30 ml) and extracted with EtOAc (2×30 ml). The combined EtOAc layers were washed with water (8×30 ml) and then dried (MgSO4) and evaporated to give the title compound (D56) as an oil (0.11 g).
Description 57
4N HCl in dioxane (3 ml) was added to a solution of 1,1-dimethylethyl (3S)-3-[(4-ethyl-1-piperazinyl)carbonyl]-1-pyrrolidinecarboxylate (may be prepared as described in D56) (0.11 g) in MeOH (3 ml). The reaction mixture was left standing at room temperature overnight. The solvent was removed to give an oil which was basified in water (3 ml) and solid potassium carbonate. The oil obtained was then extracted into DCM (3×15 ml). The combined organic extracts were dried (MgSO4) and concentrated to give the title compound (D57) as an oil (52 mg). 1H NMR (methanol-d4) δ: 1.33-1.55 (3H, t), 2.0-2.49 (2H, m), 2.97-3.27 (4H, m), 3.3-3.45 (4H, m), 3.54-3.78 (5H, m), 4.25-4.37 (1H, m), 4.65-4.75 (1H, m)
5-Bromo-2-(trifluoromethyl)pyridine (may be prepared as described in Cottet and Schlosser, Eur. J. Org. Chem., 2002, 327) (0.242 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME under argon at room temperature. Potassium phosphate (0.377 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.2 g in DME) were added sequentially to bring the total volume of DME to 5 ml and the mixture heated to 75° C. for 4 h under argon. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH followed by 2M NH3/MeOH. The ammoniacal fractions were collected and evaporated and the residue purified on a silica chromatography column eluting with 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM. The pure fractions were crystallised from EtOAc to afford the title compound (E1) as pale yellow crystals (0.109 g). LCMS electrospray (+ve) 371 (MH+). 1H NMR (CDCl3) δ 1.05 (6H, d, J=6.8 Hz), 2.23-2.37 (2H, m), 2.50-2.57 (4H, m), 2.71-2.78 (1H, m), 3.38-3.68 (9H, m), 6.82 (1H, dd, J=8.8, 2.8 Hz), 7.47 (1H, d, J=8.8 Hz), 8.01 (1H, d, J=2.8 Hz).
4-Bromoacetophenone (0.213 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME under argon at room temperature. Potassium phosphate (0.377 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.2 g in DME) were added sequentially to bring the total volume of DME to 5 ml and the mixture heated to 75° C. for 4 h under argon. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH followed by 2M NH3/MeOH. The ammoniacal fractions were collected and evaporated and the residue purified on a silica chromatography column (FM(II) system) eluting with 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM. The residue was triturated with diethyl ether and crystallised from ethyl acetate to afford the title compound (E2) as pale yellow crystals (0.109 g). LCMS electrospray (+ve) 344 (MH+). 1H NMR (CDCl3) δ 1.05 (6H, d, J=6.8 Hz), 2.21-2.38 (2H, m), 2.49 (3H, s), 2.50-2.57 (4H, m), 2.70-2.76 (1H, m), 3.37-3.68 (9H, m), 6.52 (2H, d, J=8.8 Hz), 7.87 (2H, d, J=8.8 Hz).
5-(4-Bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) (0.255 g) was added to a stirred solution of tris(dibenzylideneacetone)dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME (4 ml) under argon at room temperature. Potassium phosphate (0.377 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.2 g in 1 ml DME) were added sequentially and the mixture heated to 75° C. for 4 h. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (70 ml) followed by 2M NH3/MeOH (70 ml). The ammoniacal fractions were collected and evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The pure fractions were evaporated and the residue triturated with diethyl ether to afford the title compound (E3) as a white solid (0.085 g). LCMS electrospray (+ve) 384 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.8 Hz), 2.21-2.39 (2H, m), 2.42 (3H, s), 2.50-2.56 (4H, m), 2.70-2.77 (1H, m), 3.38-3.68 (9H, m), 6.59 (2H, d, J=8.8 Hz), 7.95 (2H, d, J=8.8 Hz).
5-(4-Bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) (1.55 g) was added to a stirred solution of tris(dibenzylideneacetone)dipalladium(0) (0.124 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.16 g) in DME (10 ml) under argon at room temperature. Potassium phosphate (2.3 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (1.22 g in 10 ml DME) were added sequentially and the mixture heated to 75° C. Further portions of tris(dibenzylideneacetone)dipalladium(0) (0.124 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.16 g) were added to the reaction after both 1.5 h and 4.5 h of heating. After 16 h the mixture was allowed to cool, filtered through a pad of celite and the pad washed with MeOH. The solvent was evaporated and the residue purified by flash chromatography [silica gel, gradient elution: DCM/0-5% 2M ammonia in MeOH]. The pure fractions were evaporated and the residue triturated with diethyl ether. The free base was then dissolved in a minimum volume of EtOAc and 1M HCl/diethyl ether (4 ml) added. The solvents were removed and the residue re-evaporated from acetone (×3). The residue was then dissolved in a minimum volume of hot ethanol and the hot solution rapidly filtered. The product was allowed to crystallise from solution, filtered off and washed with cold ethanol. The product was then re-crystallised from hot ethanol following the same procedure to afford the title compound (E3A) as a colourless, crystalline solid. LCMS electrospray (+ve) 384 (MH+). 1H NMR (DMSO-d6) δ 1.29 (6H, d, J=6.4 Hz), 2.10-2.25 (2H, m), 2.34 (3H, s), 2.93 (1H, m), 3.05-3.16 (2H, m), 3.33-3.67 (9H, m), 4.25 (1H, m), 4.52 (1H, m), 6.70 (2H, d, J=8.8 Hz), 7.86 (2H, d, J=8.8 Hz) and 10.75 (1H, bs). Enantiomeric excess=99.8% (Chiralcel OJ (250×4.6 mm, 10 micron particle size); Heptane:Ethanol 50:50 v/v; isocratic for 25 min at 1 ml/min). Retention time of major component=19.4 min, minor component=12.0 min.
5-Bromo-2-(trifluoromethyl)pyridine (may be prepared as described in Cottet and Schlosser, Eur. J. Org. Chem., 2002, 327) (0.171 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME under argon at room temperature. Potassium phosphate (0.267 g) and (2S)-1-(1-methylethyl)-2-methyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 21) (0.15 g in DME) were added sequentially to bring the total volume of DME to 4 ml and the mixture heated to 75° C. for 4 h under argon. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH followed by 2M NH3/MeOH. The ammoniacal fractions were collected and evaporated and the residue purified on silica chromatography column (FM(II) system) eluting with 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The residue was triturated with ether and dried to afford the title compound (E4) as a white solid (0.035 g). LCMS electrospray (+ve) 385 (MH+). 1H NMR (CDCl3) δ 0.89 (3H, d, J=6.8 Hz), 1.08 (3H, dd, J=14.4, 6.4 Hz), 1.13 (3H, d, J=6.8 Hz), 2.22-2.36 (3H, m), 2.54-3.08 (3H, m), 3.20-3.81 (8H, m), 4.21-4.31 (1H, m), 6.82 (1H, dd, J=8.8, 2.4 Hz), 7.47 (1H, d, J=8.8 Hz), 8.02 (1H, d, J=2.4 Hz).
Method A
4-Bromoacetophenone (0.151 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME under argon at room temperature. Potassium phosphate (0.267 g) and (2S)-1-(1-methylethyl)-2-methyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 21) (0.15 g in DME) were added sequentially to bring the total volume of DME to 4 ml and the mixture heated to 75° C. for 4 h under argon. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH followed by 2M NH3/MeOH. The ammoniacal fractions were collected and evaporated and the residue purified on a silica chromatography column (FM(II) system) eluting with 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM. The pure fractions were triturated with ether and dried to afford the title compound (E5) as a pale yellow solid (0.055 g). LCMS electrospray (+ve) 358 (MH+). 1H NMR (CDCl3) δ 0.89 (3H, dd, J=6.4, 3.2 Hz), 1.08 (3H, dd, J=14.0, 6.0 Hz), 1.12 (3H, d, J=6.8 Hz), 2.21-2.38 (3H, m), 2.50 (3H, s), 2.52-3.08 (3H, m), 3.21-3.81 (8H, m), 4.22-4.31 (1H, m), 6.52 (2H, d, J=8.0 Hz), 7.86 (2H, d, J=8.0 Hz).
Method B
4-Bromoacetophenone (0.167 g) was dissolved in DME (2 ml). Tris(dibenzylideneacetone) dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.072 g) were added and the mixture was stirred briefly before addition of (2S)-1-(1-methylethyl)-2-methyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 21) (0.2 g) in DME (2 ml). Potassium phosphate (0.365 g) was then added and the reaction was heated to 80° C. for 6 h under argon. After cooling, the reaction mixture was filtered and diluted with MeOH (20 ml). The solution was loaded onto a 10 g SCX cartridge and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were collected and stripped. The residue was divided into two portions and purified on two 12M silica columns. The columns were run on Biotage SP1 eluting with a gradient of 0-50% MeOH in ethyl acetate over 20 column volumes. The pure fractions were combined and evaporated. The resulting solid was dissolved in MeOH (4 ml) and treated with ethereal HCl (2 ml). The solvent was blown down to give the title product (E5) (0.018 g) as a light brown solid. LCMS electrospray (+ve) 358 (MH+). 1H NMR (CDCl3) δ 0.89 (3H, dd, J=6.4, 3.2 Hz), 1.08 (3H, dd, J=14.0, 6.0 Hz), 1.12 (3H, d, J=6.8 Hz), 2.21-2.38 (3H, m), 2.50 (3H, s), 2.52-3.08 (3H, m), 3.21-3.81 (8H, m), 4.22-4.31 (1H, m), 6.52 (2H, d, J=8.0 Hz), 7.86 (2H, d, J=8.0 Hz).
Tris(dibenzylideneacetone)dipalladium (0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.072 g) were added to a stirred solution of 4-bromoacetophenone (0.167 g) in DME (2 ml) under argon followed by the sequential addition of (2S)-1(1-methylethyl)-2-methyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 21) (0.2 g) in DME (2 ml) and potassium phosphate (0.365 g). The reaction mixture was heated to 80° C. for 6 h. After cooling, the mixture was diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were collected and evaporated to give a crude product (0.295 g) which was purified by chromatography (silica-gel; 0-50% MeOH/EtOAc). The pure fractions were collected and evaporated to give the free base which was dissolved in dry MeOH (4 ml) and treated with 1N ethereal HCl (2 ml). Evaporation of solvents gave the title compound (E5A) as a light brown solid (18 mg). LCMS electrospray (+ve ) 358 (MH+). 1H NMR (methanol-d4) δ: 1.25 (3H, m), 1.37-1.59 (6H, m), 2.25-2.5 (4H, m), 2.88-3.20 (3H, m), 3.45-3.71 (8H, m), 3.95-4.05 (1H, m), 4.33-4.43 (1H, m), 4.61-4.8 (1H, m), 6.58 (2H, d, J=8.0 Hz), 7.88 (2H, d, J=8.0 Hz).
The title compound (E6) was prepared from 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) and (2S)-1-(1-methylethyl)-2-methyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 21) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 398 (MH+). 1H NMR (CDCl3) δ 0.89 (3H, dd, J=6.4, 3.6 Hz), 1.08 (3H, dd, J=15.2, 6.4 Hz), 1.13 (3H, d, J=6.8 Hz), 2.23-2.38 (3H, m), 2.43 (3H, s), 2.52-3.08 (3H, m), 3.21-3.81 (8H, m), 4.22-4.31 (1H, m), 6.59 (2H, d, J=8.4 Hz), 7.94 (2H, d, J=8.4 Hz).
5-(4-Fluorophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 55) (0.158 g) and potassium carbonate (0.204 g) were added to a mixture of (2S)-1-(1-methylethyl)-2-methyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 21) (0.177 g) in DMSO (4 ml) and the mixture heated to 130° C. under Argon for 2 h. The mixture was allowed to cool, diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were evaporated and the residue purified by mass directed auto-purification. The purified product was dissolved in a minimum volume of MeOH and converted to the hydrochloride salt by addition of 1M HCl/Et2O. The solvents were evaporated and the residue crystallised from a minimum volume of hot ethanol to afford the title compound (6A) as a white crystalline solid (0.04 g). LCMS electrospray (+ve) 398 (MH+). 1H NMR (DMSO-d6) δ: 1.15 (3H, d, J=6.0 Hz), 1.35 (6H, bs), 2.06-2.32 (3H, m), 2.34 (3H, s), 2.85-3.15 (2H, m), 3.21-3.71 (7H, m), 3.85 (1H, m), 4.22 (1H, m), 4.50 (1H, m), 6.70 (2H, d, J=8.4 Hz), 7.86 (2H, d, J=8.4 Hz), 10.05 (1H, bs).
The title compound (E7) was prepared from 5-bromo-2-(trifluoromethyl)pyridine (may be prepared as described in Cottet and Schlosser, Eur. J. Org. Chem., 2002, 327) and 1-(1-ethylpropyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 12) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 399 (MH+). 1H NMR (CDCl3) δ 0.91 (6H, t, J=7.6), 1.25-1.39 (2H, m), 1.40-1.51 (2H, m), 2.17-2.37 (3H, m), 2.50-2.56 (4H, m), 3.37-3.68 (9H, m), 6.82 (1H, dd, J=8.8, 2.8 Hz), 7.46 (1H, d, J=8.8 Hz), 8.01 (1H, d, J=2.8 Hz).
4-Bromoacetophone (0.213 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME under argon at room temperature. Potassium phosphate (0.335 g) and 1-(1-ethylpropyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 12) (0.2 g in DME) were added sequentially to bring the total volume of DME to 5 ml and the mixture heated to 75° C. for 4 h under argon. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH followed by 2M NH3/MeOH. The ammoniacal fractions were collected and evaporated and the residue purified on a silica chromatography column (FM(II) system) eluting with 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM. The residue was precipitated from ethyl acetate to afford the title compound (E8) (0.08 g) as a pale yellow powder. LCMS electrospray (+ve) 372 (MH+). 1H NMR (CDCl3) δ 0.91 (6H, t, J=7.6 Hz), 1.28-1.35 (2H, m), 1.44-1.51 (2H, m), 2.19-2.37 (3H, m), 2.50 (3H, s), 2.51-2.57 (4H, m), 3.37-3.66 (9H, m), 6.51 (2H, d, J=9.2 Hz), 7.86 (2H, d, J=9.2 Hz).
The title compound (E9) was prepared from 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) and 1-(1-ethylpropyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 12) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 412 (MH+). 1H NMR (CDCl3) δ 0.92 (6H, t, J=7.6 Hz), 1.30-1.36 (2H, m), 1.41-1.49 (2H, m), 2.17-2.39 (3H, m), 2.42 (3H, s), 2.50-2.58 (4H, m), 3.38-3.66 (9H, m), 6.60 (2H, d, J=8.8 Hz), 7.94 (2H, d, J=8.8 Hz).
The title compound (E10) was prepared from 4-bromoacetophenone and 1-(cyclopropylmethyl)-4-[(3S)-pyrrolidin-3-ylcarbonyl]-hexahydro-1H-1,4-diazepine (may be prepared as described in Description 16) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 370 (MH+). 1H NMR (CDCl3) δ 0.05-0.12 (2H, m), 0.46-0.52 (2H, m), 0.80-0.91 (1H, m), 1.88-2.00 (2H, m), 2.21-2.42 (4H, m), 2.50 (3H, s), 2.69-2.85 (4H, m), 3.37-3.46 (2H, m), 3.55-3.71 (7H, m), 6.51 (2H, d, J=8.8 Hz), 7.86 (2H, d, J=8.8 Hz).
The title compound (E11) was prepared from 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) and 1-(cyclopropylmethyl)-4-[(3S)-pyrrolidin-3-ylcarbonyl]-hexahydro-1H-1,4-diazepine (may be prepared as described in Description 16) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 410 (MH+). 1H NMR (CDCl3) δ 0.07-0.12 (2H, m), 0.49-0.54 (2H, m), 0.82-0.91 (1H, m), 1.88-2.00 (2H, m), 2.22-2.42 (4H, m), 2.42 (3H, s), 2.70-2.86 (4H, m), 3.38-3.47 (2H, m), 3.56-3.72 (7H, m), 6.58 (2H, d, J=8.8 Hz), 7.94 (2H, d, J=8.8 Hz).
The title compound (E12) was prepared from 5-bromo-2-(trifluoromethyl)pyridine (may be prepared as described in F. Cottet and M. Schlosser, Eur. J. Org. Chem., 2002, 327) and 1-cyclobutyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 10) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 383 (MH+). 1H NMR (CDCl3) δ 1.68-1.78 (2H, m), 1.83-1.93 (2H, m), 2.02-2.09 (2H, m), 2.22-2.39 (6H, m), 2.73 (1H, m), 3.38-3.68 (9H, m), 6.82 (1H, dd, J=8.8, 2.8 Hz), 7.47 (1H, d, J=8.8 Hz), 8.01 (1H, d, J=2.8 Hz).
The title compound (E13) was prepared from 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) and 1-cyclobutyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 10) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 396 (MH+). 1H NMR (CDCl3) δ 1.69-1.78 (2H, m), 1.83-1.94 (2H, m), 2.02-2.09 (2H, m), 2.21-2.42 (6H, m), 2.61 (3H, s), 2.73 (1H, m), 3.38-3.67 (9H, m), 6.58 (2H, d, J=8.8 Hz), 7.94 (2H, d, J=8.8 Hz).
The title compound (E14) was prepared from 5-bromo-2-(trifluoromethyl)pyridine (may be prepared as described in Cottet and Schlosser, Eur. J. Org. Chem., 2002, 327) and 1-(cyclopropylmethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 28) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 383 (MH+). 1H NMR (CDCl3) δ 0.05-0.12 (2H, m), 0.51-0.57 (2H, m), 0.80-0.91 (1H, m), 2.23-2.39 (4H, m), 2.51-2.59 (4H, m), 3.39-3.75 (9H, m), 6.82 (1H, dd, J=8.8, 2.8 Hz), 7.47 (1H, d, J=8.8 Hz), 8.01 (1H, d, J=2.8 Hz).
The title compound (E15) was prepared from 4-bromoacetophenone and 1-(cyclopropylmethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 28) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 356 (MH+). 1H NMR (CDCl3) δ 0.05-0.12 (2H, m), 0.51-0.57 (2H, m), 0.80-0.91 (1H, m), 2.23-2.39 (4H, m), 2.50 (3H, s), 2.51-2.59 (4H, m), 3.36-3.71 (9H, m), 6.51 (2H, d, J=8.8 Hz), 7.86 (2H, d, J=8.8 Hz).
The title compound (E16) was prepared from 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) and 1-(cyclopropylmethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 28) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 396 (MH+). 1H NMR (CDCl3) δ 0.05-0.12 (2H, m), 0.51-0.57 (2H, m), 0.80-0.91 (1H, m), 2.22-2.38 (4H, m), 2.42 (3H, s), 2.52-2.59 (4H, m), 3.37-3.71 (9H, m), 6.59 (2H, d, J=8.8 Hz), 7.94 (2H, d, J=8.8 Hz).
5-Bromo-2-(trifluoromethyl)pyridine (may be prepared as described in Cottet and Schlosser, Eur. J. Org. Chem., 2002, 327) (0.95 g) in dry and degassed dioxan (40 ml) was treated with bis(dibenzylideneacetone)palladium (0.64 g) and 2-dicyclohexylphosphine-2′-(N,N-dimethylamino)biphenyl (0.71 g) and stirred at room temperature for 20 min followed by the addition of (R,S)-1-cyclobutyl-4-(3-pyrrolidinylcarbonyl)hexahydro-1H-1,4-diazepine (may be prepared as described in Description 30) (1.08 g) in dioxane (10 ml) and sodium t-butoxide (0.81 g). The reaction mixture was heated at 95° C. for 2 h, then allowed to cool and diluted with MeOH (40 ml). This solution was poured on to an SCX column which was washed with MeOH (40 ml) and then eluted with ammonia in MeOH (2N, 30 ml). Concentration of the ammonia fractions afforded a crude product which was further purified by chromatography [silica gel, eluting with 0-10% MeOH (containing 10% 0.880 ammonia solution)/DCM]. Pure fractions were combined and concentrated to give the title compound (E17) as a brown oil (0.350 g). LCMS electrospray (+ve) 397 (MH+). Two enantiomers: Retention times=5.3 min; 6.6 min [Chiralcel OJ column (250 mm×4.6 mm, 10 micron particle size); hexane:ethanol 60:40 v/v; isocratic for 10 min at 1 ml/min).
(R,S)-1-Cyclobutyl-4-({1-[6-(trifluoromethyl)-3-pyridinyl]-3-pyrrolidinyl}carbonyl) hexahydro-1H-1,4-diazepine (may be prepared as described in Example 17) (0.35 g) was separated by chiral HPLC [Stationary phase: Chiralcel OJ column (250 mm×50 mm i.d.; 20 micron particle size); Mobile phase: hexane fraction:absolute ethanol 90:10 v/v; isocratic at a flow rate of 50 ml/min; detection by UV absorbance at 215 nm; sample injected as a solution in absolute ethanol]. Fractions containing the faster running enantiomer (retention time=31.1 min) were combined and concentrated to give the free base which was dissolved in dry DCM (5 ml) and treated with 1N ethereal HCl (1 ml). Evaporation of solvents afforded the title compound (E17A) as a cream solid (0.077 g). 1H NMR δ (methanol-d4): 1.7-1.95 (2H, m), 2.2-2.45 (8H, m), 2.9-3.2 (2H, m), 3.47-3.68 (8H, m), 3.75-3.9 (3H, m), 4.1-4.25 (1H, m), 7.07 (1H, dd, J=8 Hz), 7.57 (1H, d, J=8 Hz), 7.97 (1H, d, J=2.8 Hz). LCMS electrospray 397 (MH+).
The slower running enantiomer (retention time=41.6 min) was isolated and converted into the HCl salt as described above to give the title compound (E17B) as a cream coloured solid (0.1 g). 1H NMR δ (methanol-d4): 1.7-1.95 (2H, m), 2.2-2.45 (8H, m), 2.9-3.2 (2H, m), 3.47-3.68 (8H, m), 3.75-3.9 (3H, m), 4.1-4.25 (1H, m), 7.25 (1H, m), 7.70 (1H, m), 8.03 (1H, d, J=2.8 Hz). LCMS electrospray 397 (MH+).
Alternative Method
The title compound (E17A) was prepared in an analogous manner to Example 1 from 5-bromo-2-(trifluoromethyl)pyridine (may be prepared as described in F Cottet and M Schlosser, Eur. J. Org. Chem., 2002, 327) and 1-cyclobutyl-4-[(3R)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 38). LCMS electrospray (+ve) 397 (MH+). 1H NMR (CDCl3) δ 1.56-2.07 (8H, m), 2.25-2.52 (6H, m), 2.81-2.99 (1H, m), 3.35-3.69 (9H, m), 6.82 (1H, dd, J=8.7 and 2.8 Hz), 7.46 (1H, d, J=8.7 Hz), 8.02 (1H, d, J=2.8 Hz). Enantiomeric excess=90% (Chiralcel OJ (250×4.6 mm, 10 micron particle size); Hexane:Ethanol 60:40 v/v; isocratic for 10 min at 1 ml/min). Retention time of major component=5.3 min, minor component=6.6 min.
(R,S)-1-Cyclobutyl-4-({1-[4-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl]-3-pyrrolidinyl}carbonyl)hexahydro-1H-1,4-diazepine was prepared using an analogous process to that described in Example 17 from (R,S)-1-cyclobutyl-4-(3-pyrrolidinylcarbonyl)hexahydro-1H-1,4-diazepine (may be prepared as described in Description 30) and 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23). The racemic product (300 mg) was separated by chiral HPLC [Stationary phase: Chiralcel OJ column [250 mm×50 mm i.d., 20 micron particle size; mobile phase: 100% ethanol, isocratic at a flow rate of 50.0 mL/min; detection by UV absorbance at 215 nm; sample injected as a solution in ethanol. Fractions containing the faster running enantiomer (E18A) (retention time=8.03 min) were combined and concentrated to give the free base which was treated with ethereal HCl to give the hydrochloride salt as a cream solid (104 mg) 1H NMR δ (methanol-d4): 1.77-1.95 (2H, m), 2.2-2.35 (7H, m), 2.4 (3H, s), 2.9-3.15, (2H, m), 3.47-3.90 (12H, m), 4.1-4.25 (1H, m), 6.7 (2H, d, J=8.8 Hz), 7.9 (2H, d, J=8.8 Hz). LCMS electrospray (+ve) 410 (MH+). Fractions containing the slower running enantiomer (E18B) (retention time=13.0 min) were combined and concentrated to give the free base which was treated with ethereal HCl to give the hydrochloride salt as a cream solid (81 mg). 1H NMR δ (methanol-d4): 1.77-1.95 (2H, m), 2.2-2.35 (7H, m), 2.4 (3H, s), 2.9-3.15 (2H, m), 3.47-3.90 (12H, m), 4.1-4.25 (1H, m), 6.7 (2H, d, J=8.8 Hz), 7.9 (2H, d, J=8.8 Hz). LCMS electrospray (+ve) 410 (MH+).
(R,S)-6-{3-[(4-Cyclobutylhexahydro-1H-1,4-diazepin-1-yl)carbonyl]-1-pyrrolidinyl}-2-methylquinoline was prepared in using an analogous process to that described in Example 17 from (R,S)-1-cyclobutyl-4-(3-pyrrolidinylcarbonyl)hexahydro-1H-1,4-diazepine (may be prepared as described in Description 30) and 6-bromo-2-methylquinoline. The racemic product (0.5 g) was separated by chiral HPLC [Stationary phase: Chiralcel OJ column (250 mm×20 mm i.d., 10 micron particle size); mobile phase: 100% ethanol]; isocratic flow rate of 17 ml/min; detection by UV absorbance at 215 nm; sample injected as a solution in ethanol]. Fractions containing the faster running enantiomer (E19A) (retention time=8.03 min) were combined and concentrated to give the free base which was treated with ethereal HCl to give the hydrochloride salt as a yellow solid (186 mg). 1H NMR δ (methanol-d4) 1.78-1.95 (2H, m), 2.35-2.5 (7H, m), 2.87 (3H, s), 2.9-3.15 (2H, m), 3.45-3.90 (12, m), 4.10-4.25 (1H, m), 7.05 (1H, s), 7.6 (1H, d, J=8.8 Hz), 7.69 (1H, d, J=8.8 Hz), 7.94 (1H, d, J=8.8 Hz), 8.67 (1H, d, J=8.8 Hz). LCMS AP+ (+ve) 393 (MH+). Fractions containing the slower running enantiomer (E19B) (retention time=13.0 min) were combined and concentrated to give the free base which was treated with ethereal HCl to give the hydrochloride salt as a yellow solid (197 mg). 1H NMR δ (methanol-d4): 1.78-1.95 (2H, m), 2.25-2.5 (7H, m), 2.87 (3H, s), 2.9-3.15 (2H, m), 3.45-3.90 (12, m), 4.10-4.25 (1H, m), 7.05 (1H, s), 7.6 (1H, d, J=8.8 Hz), 7.69 (1H, d, J=8.8 Hz), 7.94 (1H, d, J=8.8 Hz), 8.67 (1H, d, J=8.8 Hz). LCMS AP+ (+ve) 393 (MH+).
(R,S)-1-Cyclobutyl-4-({1-[2-fluoro-4-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl]-3-pyrrolidinyl}carbonyl)hexahydro-1H-1,4-diazepine was prepared using an analogous process to that described in Example 17 from (R,S)-1-cyclobutyl-4-(3-pyrrolidinylcarbonyl)hexahydro-1H-1,4-diazepine (may be prepared as described in Description 30) and 5-(4-bromo-3-fluorophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 31). The racemic product (0.09 g) was separated by chiral HPLC [Stationary phase: Chiral OJ column (250 mm×50 mm i.d., 20 micron particle size); mobile phase: 100% ethanol at a flow rate of 50 mL/min; detection by UV absorbance at 215 nm; sample injected as a solution in ethanol]. Fractions containing the faster running enantiomer (E20A) (retention time=6.6 min) were combined and concentrated to give the free base which was treated with 1M ethereal HCl to give a cream solid (17.8 mg). 1H NMR δ (methanol-d4): 1.78-1.95 (2H, m), 2.25-2.5 (7H, m), 2.87 (3H, s), 2.9-3.15 (2H, m), 3.45-3.90 (12H, m), 4.10-4.25 (1H, m), 6.8 (1H, t, J=8.8 Hz), 7.65 (1H, d, J=14.8 Hz), 7.75 (1H, d, J=8.8 Hz). LCMS electrospray (+ve) 428 (MH+). Fractions containing the slower running enantiomer (E20B) (retention time=9.4 min) were combined and concentrated to give the free base which was treated with 1M ethereal HCl to give a cream solid (17 mg). 1H NMR δ (methanol-d4): 1.78-1.95 (2H, m), 2.25-2.5 (7H, m), 2.87 (3H, s), 2.9-3.15 (2H, m), 3.45-3.90 (12, m), 4.10-4.25 (1H, m), 6.8 (1H, t, J=8.8 Hz), 7.65 (1H, d, J=14.8 Hz), 7.75 (1H, d, J=8.8 Hz). LCMS electrospray (+ve) 428 (MH+).
(R,S)-1-(4-{3-[(4-Cyclobutylhexahydro-1H-1,4-diazepin-1-yl)carbonyl]-1-pyrrolidinyl}phenyl)ethanone was prepared using an analogous process to that described in Example 17 from (R,S)-1-cyclobutyl-4-(3-pyrrolidinylcarbonyl)hexahydro-1H-1,4-diazepine (may be prepared as described in Description 30) and 4-fluoroacetophenone. The racemic product (0.39 g) was separated by chiral HPLC [Stationary phase: Chiralcel OJ column (250 mm×50 mm i.d., 20 micron particle size); mobile phase: 100% ethanol at a flow rate of 50 ml/min; detection by UV absorbance at 215 nm; sample injected as a solution in ethanol]. Fractions containing the faster running enantiomer (E21A) (retention time=6.7 min) were combined and concentrated to give the free base which was treated with 1M ethereal HCl to give a cream solid (25 mg). 1H NMR δ (methanol-d4): 1.78-1.95 (2H, m), 2.15-2.45 (7H, m), 2.5 (3H, s), 2.9-3.15 (2H, m), 3.45-3.90 (12H, m), 4.10-4.25 (1H, m), 6.6 (2H, d, J=9.2 Hz), 7.85 (2H, d, J=9.2 Hz).
LCMS electrospray (+ve) 370 (MH+). Fractions containing the slower running enantiomer (E21B) (retention time=8.7 min) were combined and concentrated to give the free base which was treated with 1M ethereal HCl to give a cream solid (20 mg). 1H NMR δ (methanol-d4): 1.78-1.95 (2H, m), 2.15-2.45 (7H, m), 2.5 (3H, s), 2.9-3.15 (2H, m), 3.45-3.90 (12, m), 4.10-4.25 (1H, m), 6.6 (2H, d, J=9.2 Hz), 7.85 (2H, d, J=9.2 Hz). LCMS electrospray (+ve) 370 (MH+).
5-Bromo-2-(trifluoromethyl)pyridine (may be prepared as described in F. Cottet and M. Schlosser, Eur. J. Org. Chem., 2002, 327) (0.156 g) was added to a solution of tris(dibenzylideneacetone)dipalladium(0) (0.03 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.06 g) in dry, degassed 1,4-dioxane (2.5 ml) and the mixture stirred for 40 min. at room temperature under argon. (R,S)-1-(1-methylethyl)-4-(3-pyrrolidinylcarbonyl)hexahydro-1H-1,4-diazepine (may be prepared as described in Description 36) (0.15 g in 2.5 ml DME) and sodium tert-butoxide (0.12 g) were added sequentially and the mixture heated to 90° C. for 2 h. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (70 ml) followed by 2M NH3/MeOH (70 ml). The ammoniacal fractions were collected and evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The pure fractions were evaporated and the free base converted into the title hydrochloride salt (E22) with 1M HCl in diethyl ether. LCMS electrospray (+ve) 385 (MH+). 1H NMR (DMSO-d6) δ: 1.27 (6H, d, J=6.5 Hz), 1.99-2.42 (5H, m), 2.98-3.28 (2H, m), 3.35-3.76 (10H, m), 3.95-4.22 (1H, m), 7.00 (1H, dd, J=8.6 and 2.0 Hz), 7.59 (1H, d, J=8.6 Hz), 8.05 (1H, d, J=2.0 Hz), 10.20-10.40 (1H, bs).
The title compound (E23) was prepared using an analogous process to that described in Example 22 from 4-iodo-2-(trifluoromethyl)pyridine (may be prepared as described in F Cottet et al., Eur. J. Org. Chem., 2003, 1559) and (R,S) 1-cyclobutyl-4-(3-pyrrolidinylcarbonyl)hexahydro-1H-1,4-diazepine (may be prepared as described in Description 30). LCMS electrospray (+ve) 397 (MH+). 1H NMR (methanol-d4) δ: 1.72-1.99 (2H, m), 2.15-2.53 (8H, m), 2.84-3.20 (2H, m), 3.51-3.90 (10H, m), 3.99-4.24 (2H, m), 6.99 (1H, dd, J=7.1 and 2.6 Hz), 7.25 (1H, d, J=2.6 Hz), 8.19 (1H, d, J=7.1 Hz).
5-(4-Bromo-2-fluorophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 32) (0.275 g) was added to a stirred solution of tris(dibenzylideneacetone)dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME (4 ml) under argon at room temperature. Potassium phosphate (0.377 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.2 g in 1 ml DME) were added sequentially and the mixture heated to 75° C. for 4 h. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (70 ml) followed by 2M NH3/MeOH (70 ml). The ammoniacal fractions were collected and evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The pure fractions were evaporated and the residue triturated with diethyl ether to afford the title compound (E24) as a white solid (0.055 g). LCMS electrospray (+ve) 402 (MH+). 1H NMR (CDCl3) δ 1.05 (6H, d, J=6.8 Hz), 2.21-2.39 (2H, m), 2.45 (3H, s), 2.50-2.57 (4H, m), 2.70-2.77 (1H, m), 3.38-3.67 (9H, m), 6.31 (1H, dd, J=114.0 and 2.4 Hz), 6.40 (1H, dd, J=8.8 and 2.4 Hz), 7.89 (1H, t, J=8.8 Hz).
5-(4-Bromo-3-fluorophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 31) (0.275 g) was added to a stirred solution of tris(dibenzylideneacetone)dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME (4 ml) under argon at room temperature. Potassium phosphate (0.377 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.2 g in 1 ml DME) were added sequentially and the mixture heated to 75° C. for 4 h. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (70 ml) followed by 2M NH3/MeOH (70 ml). The ammoniacal fractions were collected and evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The pure fractions were evaporated and the residue triturated with diethyl ether (×2) to afford the title compound (E25) as a white solid (0.050 g). LCMS electrospray (+ve) 402 (MH+). 1H NMR (CDCl3) δ 1.05 (6H, d, J=6.8 Hz), 2.17-2.32 (2H, m), 2.42 (3H, s), 2.49-2.56 (4H, m), 2.70-2.77 (1H, m), 3.32-3.67 (9H, m), 6.67 (1H, t, J=8.4 Hz), 7.68 (1H, dd, J=14.4 and 2.0 Hz), 7.73 (1H, dd, J=8.4 and 2.0 Hz).
5-Bromo-2-(trifluoromethyl)pyridine (may be prepared as described in Cottet and Schlosser, Eur. J. Org. Chem., 2002, 327) (0.226 g) was added to a stirred solution of tris(dibenzylideneacetone)dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in DME (4 ml) under argon at room temperature. Potassium phosphate (0.354 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) (0.2 g) in DME (1 ml) were added sequentially and the mixture heated to 75° C. for 4 h. The mixture was then diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (70 ml) followed by 2M NH3/MeOH (70 ml). The ammoniacal fractions were combined, evaporated and the residue purified by flash chromatography [silica gel, 0-10% MeOH (containing 10% 0.88 ammonia solution)/DCM]. The clean fractions were evaporated and the residue dissolved in a minimum of EtOAc. 1M HCl/diethylether (0.3 ml) was added and evaporation of the solvents afforded the title compound (E26) as a pale yellow solid (0.06 g). LCMS electrospray (+ve) 385 (MH+). 1H NMR (CDCl3) δ: 1.44 (6H, d, J=6.0 Hz), 2.01-2.52 (5H, m), 2.80-3.08 (2H, m), 3.24 (1H, m), 3.45-3.99 (9H, m), 4.25-4.41 (1H, m), 6.89 (1H, m), 7.51 (1H, d, J=8.4 Hz), 8.04 (1H, dd, J=9.2 and 2.0 Hz), 12.35-12.6 (1H, bm).
The title compound (E27) was prepared from 4-bromoacetophenone and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 358 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, m), 1.76-1.91 (2H, m), 2.21-2.28 (1H, m), 2.31-2.41 (1H, m), 2.50 (3H, s), 2.59-2.75 (4H, m), 2.89-2.96 (1H, m), 3.35-3.46 (2H, m), 3.54-3.68 (7H, m), 6.51 (2H, d, J=8.8 Hz) and 7.86 (2H, d, J=8.8 Hz).
5-(4-Bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) (0.240 g), potassium phosphate (0.354 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) (0.2 g in 1 ml DME) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.055 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.07 g) in dry DME (4 ml). The mixture was heated to 70° C. for 3.5 hours under argon. The crude mixture was diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH followed by 2M NH3/MeOH. The residue was purified on a silica chromatography column (FM(II) system) eluting with a gradient of 0-30% MeOH in DCM. The pure fractions were collected and evaporated to afford the title compound
(E28) as a pale yellow solid (0.085 g). LCMS electrospray (+ve) 398 (MH+). 1H NMR (CDCl3) δ 1.05 (6H, m), 1.76-1.91 (2H, m), 2.21-2.29 (1H, m), 2.31-2.42 (1H, m), 2.42 (3H, s), 2.58-2.75 (4H, m), 2.89-2.96 (1H, m), 3.36-3.48 (2H, m), 3.55-3.67 (7H, m), 6.58 (2H, d, J=8.8 Hz) and 7.94 (2H, d, J=8.8 Hz).
The title compound (E29) was prepared from 5-(4-bromo-2-fluorophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 32) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) using an analogous process to that described in Example 26. LCMS electrospray (+ve) 416 (MH+). 1H NMR (DMSO-d6) δ 1.05 (6H, m), 1.07-2.38 (4H, m), 2.38 (3H, s), 2.99-3.23 (2H, m), 3.40-3.80 (11H, m), 3.93-4.05 (1H, m), 6.50-6.57 (2H, m), 7.85 (1H, t, J=8.8 Hz) and 10.1 (1H, m).
2-(4-Bromophenyl)-5-methyl-1,3,4-oxadiazole, (may be prepared as described in Description 14 of WO9743262) (0.33 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.076 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.098 g) in DME (4 ml). Potassium phosphate (0.470 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.25 g in 1 ml DME) were added sequentially and the mixture was heated to 75° C. for 6 hours under argon. After cooling, the reaction mixture was diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (30 ml) followed by 2M NH3/MeOH (30 ml). The ammoniacal fractions were evaporated and the residue co-evaporated from methanol (×2). The residue was then triturated with ether (×3) to afford the title compound (E30) as a beige solid (0.171 g). LCMS electrospray (+ve) 384 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.4 Hz), 2.21-2.39 (2H, m), 2.50-2.57 (7H, m), 2.71-2.77 (1H, m), 3.36-3.48 (2H, m), 3.52-3.68 (7H, m), 6.59 (2H, d, J=8.8 Hz), 7.85 (2H, d, J=8.8 Hz).
The title compound (E31) was prepared from 5-(3-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 48) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) using an analogous process to that described in Example 26. LCMS electrospray (+ve) 384 (MH+). 1H NMR (Methanol-d4) δ 1.41 (6H, d, J=6.8 Hz), 2.26-2.36 (2H, m), 2.42 (3H, s), 3.09-3.25 (3H, m), 3.43-3.69 (9H, m), 4.41 (1H, m), 4.76 (1H, m), 6.91 (1H, m), 7.37 (1H, s) and 7.39 (2H, m).
4-(4-Bromophenyl)-2-methyl-1,3-oxazole (may be prepared as described in Description 43) (0.476 g), 1-(1-methylethyl)-4-[(3-pyrrolidinylcarbonyl]piperazine (0.45 g) (may be prepared in an analogous manner to that described in Description 5 and Description 6 from 1-(tert-butoxycarbonyl)-pyrrolidine-3-carboxylic acid, tris(dibenzylideneacetone)dipalladium(0) (0.137 g), 2-(dicyclohexylphosphino)biphenyl (0.158 g) and potassium phosphate (0.848 g) were heated together in dioxane (8 ml) under an argon atmosphere at 90° C. for 16 h. The mixture was cooled and diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (100 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were collected and evaporated. The residue was purified by chromatography on silica (Biotage, KP-NH cartridge) eluting with 0-100% EtOAc in hexane. The clean fractions were evaporated and the residue recrystallised from EtOH to afford the title compound (E32) as colourless needles (0.162 g). LCMS electrospray (+ve) 383 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.8 Hz), 2.18-2.24 (1H, m), 2.34-2.39 (1H, m), 2.49 (3H, s), 2.49-2.56 (4H, m), 2.70-2.76 (1H, m), 3.35-3.42 (2H, m), 3.47-3.68 (7H, m), 6.58 (2H, d, J=8.8 Hz), 7.56 (2H, d, J=8.8 Hz) and 7.66 (1H, s).
Method A
4-(4-Bromophenyl)-2-methyl-1,3-oxazole (may be prepared as described in Description 43, method A) (0.329 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.076 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.098 g) in 4 ml DME. Potassium phosphate (0.47 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6, method B) (0.25 g in 1 ml DME) were added sequentially and the mixture heated to 75° C. for 6 h under an argon atmosphere. The mixture was cooled and diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (30 ml) followed by 2M NH3/MeOH (30 ml). The ammoniacal fractions were collected and evaporated and the residue co-evaporated from methanol (×2). The residue was purified on a silica column (FM(II) system) eluting with 0-20% MeOH in DCM. The clean fractions were evaporated and the residue was triturated with diethyl ether (×3) to afford the title compound (E32A) as a pale yellow solid (0.07 g) LCMS electrospray (+ve) 383 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.8 Hz), 2.18-2.23 (1H, m), 2.32-2.38 (1H, m), 2.50 (3H, s), 2.50-2.56 (4H, m), 2.70-2.76 (1H, m), 3.35-3.42 (2H, m), 3.46-3.68 (7H, m), 6.58 (2H, d, J=8.8 Hz), 7.56 (2H, d, J=8.8 Hz) and 7.66 (1H, s).
Method B
4-(4-Bromophenyl)-2-methyl-1,3-oxazole (may be prepared as described in Description 43, method B) (0.238 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.068 g) and 2-(dicyclohexylphosphino)biphenyl (0.088 g) in dry, degassed DME (2 ml). Potassium phosphate (0.424 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6, method C) (0.225 g in 2 ml dry, degassed DME) were added sequentially and the mixture heated to 60° C. for 4.5 h followed by 3 h at 90° C. under an argon atmosphere. The mixture was cooled, dissolved in MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (60 ml) followed by 2M NH3/MeOH (60 ml). The ammoniacal fractions were collected and evaporated. The residue was purified by chromatography (KP-NH cartridge) eluting with 0-100% EtOAc in hexane. The clean fractions were evaporated and the residue recrystallised in a minimum volume of hot EtOH to afford the title compound (E32A) as colourless needles (0.1 g) LCMS electrospray (+ve) 383 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.8 Hz), 2.17-2.24 (1H, m), 2.31-2.39 (1H, m), 2.49 (3H, s), 2.49-2.56 (4H, m), 2.70-2.76 (1H, m), 3.35-3.42 (2H, m), 3.46-3.68 (7H, m), 6.58 (2H, d, J=8.8 Hz), 7.56 (2H, d, J=8.8 Hz) and 7.66 (1H, s). Enantiomeric excess=95.2% (Chiralcel OD (250×4.6 mm, 10 micron particle size); Heptane:Ethanol 50:50 v/v; isocratic for 20 min at 1 ml/min). Retention time of major component=9.28 min, minor component=15.1 min.
Method C
4-(4-Bromophenyl)-2-methyl-1,3-oxazole (may be prepared as described in Description 43, method B) (0.238 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.068 g) and 2-(dicyclohexylphosphino)biphenyl (0.088 g) in dry, degassed dioxane (2 ml). Caesium carbonate (0.650 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6, method C) (0.225 g in 2 ml dry, degassed dioxane) were added sequentially and the mixture heated to 60° C. for 4.5 h followed by 3 h at 90° C. under an argon atmosphere. The mixture was loaded onto a 10 g SCX cartridge and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were combined and evaporated. 100 mg residue was purified by chromatography (12 M amino, KP-NH cartridge). The clean fractions were evaporated to afford the title compound (E32A) as a white solid (0.04 g). (LCMS electrospray (+ve) 383 (MH+))
Method D
4-(4-Bromophenyl)-2-methyl-1,3-oxazole (may be prepared as described in Description 43, method B) (0.238 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.068 g) and 2-(dicyclohexylphosphino)biphenyl (0.088 g) in dry, degassed DME (2 ml). Caesium carbonate (0.650 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6, method C) (0.225 g in 2 ml dry, degassed DME) were added sequentially and the mixture heated to 60° C. for 4.5 h followed by 3 h at 90° C. under an argon atmosphere to afford the title compound (E32A).
Method E
4-(4-Bromophenyl)-2-methyl-1,3-oxazole (may be prepared as described in Description 43, method B) (0.238 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.068 g) and 2-(dicyclohexylphosphino)biphenyl (0.088 g) in dry, degassed dioxane (2 ml). Potassium phosphate (0.424 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6, method C) (0.225 g in 2 ml dry, degassed dioxane) were added sequentially and the mixture heated to 60° C. for 4.5 h followed by 3 h at 90° C. under an argon atmosphere to afford the title compound (E32A).
The title compound (E33) was prepared from 3-(4-bromophenyl)-5-methyl-1,2,4-oxadiazole (may be prepared as described in Description 45) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) using an analogous process to that described in Example 30. LCMS electrospray (+ve) 384 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.4 Hz), 2.18-2.26 (1H, m), 2.32-2.39 (1H, m), 2.50-2.57 (4H, m), 2.57 (3H, s), 2.71-2.77 (1H, m), 3.35-3.45 (2H, m), 3.49-3.68 (7H, m), 6.59 (2H, d, J=8.8 Hz), 7.90 (2H, d, J=8.8 Hz).
The title compound (E34) was prepared from 5-(4-bromophenyl)-2-methyl-1,3-oxazole (may be prepared as described in Description 46) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) using an analogous process to that described in Example 30. LCMS electrospray (+ve) 383 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.8 Hz), 2.18-2.25 (1H, m), 2.31-2.38 (1H, m), 2.49 (3H, s), 2.49-2.56 (4H, m), 2.70-2.77 (1H, m), 3.35-3.42 (2H, m), 3.46-3.68 (7H, m), 6.57 (2H, d, J=8.8 Hz), 6.98 (1H, s) and 7.45 (2H, d, J=8.8 Hz).
The title compound (E35) was prepared from 3-bromobenzonitrile and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) using an analogous process to that described in Example 26. LCMS electrospray (+ve) 327 (MH+). 1H NMR (DMSO-d6) δ 1.28 (6H, d, J=6.0 Hz), 2.08-2.19 (2H, m), 2.89-3.12 (3H, m), 3.28-3.59 (9H, m), 4.23 (1H, m), 4.52 (1H, m), 6.85-6.90 (2H, m), 6.98 (1H, d, J=7.6 Hz) 7.34 (1H, t, J=7.6 Hz). and 10.45 (1H, bs).
A mixture of 4-fluorobenzonitrile (0.129 g), 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.2 g) and potassium carbonate (0.245 g) in DMSO (4 ml) was heated to 130° C. for 2 h under argon. The mixture was allowed to cool, diluted with MeOH, loaded onto a 10 g SCX cartridge which was eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were evaporated and the residue purified by mass directed autopreparative HPLC . The clean fractions were evaporated, the residue taken up in a minimum of EtOAc and 1M HCl/diethyl ether (0.5 ml) added. The solvents were evaporated to afford the title compound (E36) as a white solid (0.107 g). LCMS electrospray (+ve) 327 (MH+). 1H NMR (DMSO-d6) δ 1.27 (6H, d, J=6.0 Hz), 2.10-2.23 (2H, m), 2.86-3.12 (3H, m), 3.28-3.62 (9H, m), 4.23 (1H, m), 4.51 (1H, m), 6.62 (2H, d, J=8.4 Hz), 7.54 (2H, d, J=8.4 Hz) and 10.52 (1H, bs).
5-Bromo-2-(trifluoromethyl)pyrimidine (may be prepared as described in Description 47) (0.314 g) was added to a stirred solution of tris(dibenzylideneacetone) dipalladium(0) (0.076 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.98 g in 4 ml DME) under argon at room temperature in a microwave vial. Potassium phosphate (0.470 g) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.25 g in 1 ml DME) were added sequentially and the vial capped and crimped. The reaction was heated in a microwave oven to 120° C. for 8 minutes, cooled and vented. The reaction mixture was diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (70 ml) followed by 2M NH3/MeOH (70 ml). The ammoniacal fractions were evaporated and the residue triturated with diethyl ether (×3) to afford the title compound (E37) as a white solid (0.077 g). LCMS electrospray (+ve) 372 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.4 Hz), 2.29-2.36 (2H, m), 2.50-2.57 (4H, m), 2.71-2.78 (1H, m), 3.44-3.75 (9H, m) and 8.10 (2H, s).
The title compound (E38) was prepared from 1-(1-methylethyl)-4-[(3R)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 41) and 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) using an analogous process to that described in Example 3. LCMS electrospray (+ve) 384 (MH+). 1H NMR (CDCl3) δ 1.06 (6H, d, J=6.8 Hz), 2.21-2.39 (2H, m), 2.42 (3H, s), 2.50-2.56 (4H, m), 2.70-2.77 (1H, m), 3.38-3.68 (9H, m), 6.59 (2H, d, J=8.8 Hz), 7.95 (2H, d, J=8.8 Hz). Enantiomeric excess=98.8% (Chiralcel OJ (250×4.6 mm, 10 micron particle size; Heptane:Ethanol 50:50 v/v; isocratic for 25 min at 1 ml/min). Retention time of major component=12.0 min, minor component=19.4 min.
The title compound (E39) was prepared from 1-cyclobutyl-4-[(3R)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 42) and 5-(4-bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 23) using an analogous process to that described in Example 5. LCMS electrospray (+ve) 396 (MH+). 1H NMR (CDCl3) δ 1.69-1.78 (2H, m), 1.83-1.94 (2H, m), 2.02-2.09 (2H, m), 2.21-2.42 (6H, m), 2.61 (3H, s), 2.73 (1H, m), 3.38-3.67 (9H, m), 6.58 (2H, d, J=8.8 Hz), 7.94 (2H, d, J=8.8 Hz).
2-(4-Bromophenyl)-5-phenyl-1,3,4-oxadiazole (obtainable from Lancaster 8701; 0.301 g), 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)biphenyl (0.076 g) and tris(dibenzylidene acetone)dipalladium(0) (0.060 g) were introduced into a dry carousel tube under argon. Acetonitrile (3 ml) was added, followed by a solution of 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.185 g) in acetonitrile (4.1 ml) and tripotassium phosphate (0.425 g). The stirred mixture was heated under argon, at reflux for 3 h and allowed to cool to rt. The reaction mixture was applied to an SCX column (50 g) and eluted, first with MeOH and then with 2M NH3 in MeOH. The crude product was obtained by evaporation of appropriate fractions and the title compound (E40) (0.202 g) was obtained by trituration of the crude material with Et2O. 1H NMR δ (CDCl3): 1.07 (6H, d, J=6.8 Hz), 2.20-2.29 (1H, m), 2.33-2.42 (1H, m), 2.51-2.58 (4H, m), 2.75 (1H, septet, J=6.8 Hz), 3.38-3.51 (2H, m), 3.55-3.71 (7H, m), 6.63 (2H, d, J=9 Hz), 7.52 (3H, m), 7.98 (2H, d, J=9 Hz), 8.12 (2H, m); LCMS electrospray (+ve) 446 (MH+).
Examples 41-45 were prepared using an analogous process to that described in Example 40 from 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) and the appropriate aryl bromide or iodide, and displayed 1H NMR and mass spectral data that were consistent with structure.
A mixture of 4-fluorobenzonitrile (0.160 g), 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) (0.3 g) and potassium carbonate (0.348 g) in DMSO (4 ml) was heated to 130° C. for 2 h under Argon. The mixture was allowed to cool, diluted with MeOH and loaded onto a 10 g SCX cartridge which was eluted with MeOH (40 ml) followed by 2M NH3/MeOH (40 ml). The ammoniacal fractions were evaporated and the residue purified by flash chromatography [silica gel, 0-15% MeOH/DCM]. The clean fractions were evaporated and the residue taken up in a minimum volume of DCM. A solution of 1M HCl/Et2O (0.3 ml) was added and the solvents evaporated. The residue was then recrystallised from ethanol to afford the title compound (E46) as a white crystalline solid (0.07 g). LCMS electrospray (+ve) 341 (MH+). 1H NMR (DMSO-d6) δ 1.26 (6H, d, J=6.3 Hz), 2.01-2.39 (4H, m), 2.95-3.30 (2H, m), 3.30-3.80 (11H, m), 4.03 (1H, m), 6.61 (2H, d, J=8.8 Hz), 7.54 (2H, d, J=8.8 Hz) and 10.15 (1H, bm).
The title compound (E47) was prepared from 2-(4-bromophenyl)-5-methyl-1,3,4-oxadiazole (may be prepared as described in WO9743262) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) using an analogous process to that described in Example 26. LCMS electrospray (+ve) 398 (MH+). 1H NMR (DMSO-d6) δ 1.29 (6H, m), 2.01-2.36 (4H, m), 2.52 (3H, m), 2.93-3.23 (2H, m), 3.33-3.80 (10H, m), 3.85-4.06 (2H, m), 6.67 (2H, d, J=8.8 Hz), 7.76 (2H, d, J=8.8 Hz), 10.20-10.41 (1H, m).
The title compound (E48) was prepared from 5-(4-bromophenyl)-3-ethyl-1,2,4-oxadiazole (may be prepared as described in Description 49) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) using an analogous process to that described in Example 26. LCMS electrospray (+ve) 412 (MH+). 1H NMR (CDCl3) δ 1.37 (3H, t, J=7.6 Hz), 1.42 (6H, m), 2.14-2.36 (3H, m), 2.79 (2H, q, J=7.6), 2.81-2.95 (2H, m), 3.24 (1H, m), 3.40-3.88 (11H, m), 4.40 (1H, m), 6.59 (2H, d, J=8.8 Hz), 7.96 (2H, d, J=8.8 Hz), 12.41 (1H, m).
The Title Compound (E49) was Prepared from 5-(4-Bromophenyl)-3-Ethyl-1,2,4-oxadiazole (may be prepared as described in Description 49) and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) using an analogous process to that described in Example 26. LCMS electrospray (+ve) 398 (MH+). 1H NMR (DMSO-d6) δ 1.24-1.29 (9H, m), 2.13-2.23 (2H, m), 2.71 (2H, q, J=7.6), 2.93 (1H, m), 3.09 (2H, m) 3.33-3.63 (9H, m), 4.24 (1H, m), 4.53 (1H, m) 6.69 (2H, d, J=8.8 Hz), 7.87 (2H, d, J=8.8 Hz), 10.40 (1H, m).
The title compound (E50) was prepared from 1-(4-bromophenyl)-1-propanone and 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) using an analogous process to that described in Example 37. LCMS electrospray (+ve) 358 (MH+). 1H NMR (CDCl3) δ 1.05 (3H, t, J=7.2 Hz), 1.29 (6H, m), 2.05-2.22 (2H, m), 2.88 (2H, q, J=7.2), 2.91 (1H, m), 3.01-3.22 (3H, m), 3.34-3.68 (8H, m), 4.25 (1H, m), 4.53 (1H, m), 6.58 (2H, d, J=8.8 Hz), 7.81 (2H, d, J=8.8 Hz), 10.85 (1H, bs).
5-Bromo-2-(3-methyl-1,2,4-oxadiazol-5-yl)pyridine (may be prepared as described in Description 51) (0.305 g) was added to a stirred solution of tris (dibenzylideneacetone)dipalladium (0) (0.83 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)biphenyl (0.1 g) in DME (12 ml) under argon at room temperature. Potassium phosphate (0.554 g) and 1-cyclobutyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 10) (0.3 g in 4 ml DME) were added sequentially and the mixture heated to 70° C. for 4 hours. The mixture was filtered and then diluted with MeOH (20 ml), loaded onto a 10 g SCX cartridge and eluted with MeOH (20 ml) followed by 2M NH3/MeOH. The ammoniacal fractions were collected and evaporated and the residue (0.42 g) was purified on a Waters Mass Directed Auto Preparative HPLC (eluent: 0.1% formic acid in water and 0.1% formic acid acetonitrile; gradient 10-100%). The pure fractions were evaporated and treated with 1N ethereal HCl (1 ml) in MeOH (2 ml). The solvents were removed by evaporation to give the title compound (E51) as a solid (0.105 g). 1H NMR (methanol-d4) δ: 1.33-2.00 (2H, m), 2.2-2.44 (5H, m), 2.46 (3H, m), 2.80-3.15 (3H, m), 3.5-3.8 (10H, m), 4.36-4.45 (1H, m), 4.65-4.72 (1H, m), 7.51 (1H, dd, J=9.2 Hz), 8.12 (1H, s), 8.27 (1H, d, J=9.2 Hz). LCMS electrospray (+ve) 397 (MH+).
5-(2,4-Difluorophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description 53) (0.219 g) and 1-cyclobutyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (0.263 g) (may be prepared as described in Description 10) were dissolved in DMSO (5 ml). Potassium carbonate (0.349 g) was added and the mixture was stirred and heated at 160° C. for 30 minutes. The reaction mixture was cooled, diluted with MeOH (20 ml) and loaded onto a 10 g SCX cartridge. The cartridge was washed with MeOH and eluted with 2M NH3/MeOH. The ammoniacal fractions were combined and evaporated. The residue was partitioned between DCM (20 ml) and water (20 ml). The DCM layer was separated by passing the mixture through a phase separation column and was then evaporated to give an oil which was triturated (×2) with a hexane/ether mixture to give a solid.
1H NMR (CDCl3) δ 1.67-1.80 (2H, m), 1.83-1.98 (2H, m), 2.03-2.09 (2H, m), 2.21-2.39 (6H, m), 2.45 (3H, s), 2.75 (1H, m), 3.37-3.67 (9H, m), 6.30 (1H, dd, J=14.0 and 2.4 Hz), 6.40 (1H, dd, J=8.8 and 2.4 Hz), 7.89 (1H, t, J=8.8 Hz).
The solid was dissolved in MeOH (2 ml) and treated with 1 ml ethereal HCl. The mixture was blown down to give the title compound (E52) as a solid (0.104 g). 1H NMR (methanol-d4) : 1.83-1.99 (2H, m), 2.17-2.38 (6H, m), 2.38 (3H, s), 2.59-3.30 (4H, m), 3.42-3.84 (8H, m), 4.10-4.60 (2H, 2×m), 6.44 (1H, dd, J=14.4 Hz), 6.54 (1H, dd, J=8.8 Hz), 7.87 (1H, t, J=8.8 Hz). LCMS electrospray (+ve) 414 (MH+).
5-Bromo-2-(3-methyl-1,2,4-oxadiazol-5-yl)pyridine (may be prepared as described in Description 51) (0.2 g) was added to a solution of 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]hexahydro-1H-1,4-diazepine (may be prepared as described in Description 40) (0.203 g) in DME (5 ml). Tris(dibenzylideneacetone) dipalladium (0) (0.055 g), 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.072 g) and potassium phosphate (0.364 g) were added and the reaction mixture was heated at 70° C. with stirring for 4 hours. The reaction mixture was filtered and diluted with MeOH (20 ml) The diluted mixture was then placed on a 10 g SCX column. The column was washed with MeOH and eluted with 2NH3 in MeOH. All ammoniacal fractions were combined and the solvent was evaporated. The residue was taken up in DMSO/MeCN and purified on MDAP. The relevant fractions from MDAP were combined and the solvent evaporated. The residue was then taken up in DCM (1 ml) and treated with 1M HCl in ether (2 ml). The solvent was evaporated to afford the title compound (E53). 1H NMR (methanol-d4) δ: 1.28-1.48 (6H, m), 2.21-2.40 (3H, m), 2.40-2.43 (1H, m), 2.48 (3H, s), 3.18-3.30 (2H, m), 3.51-4.25 (12H, m), 7.58 (1H, dd, J=9.2 Hz), 8.13 (1H, s), 8.31 (1H, d, J=9.2 Hz). LCMS electrospray (+ve) 399 (MH+).
5-Bromo-2-(3-methyl-1,2,4-oxadiazol-5-yl)pyridine (may be prepared as described in Description 51) (0.3 g) was dissolved in DME (5 ml). Tris(dibenzylideneacetone) dipalladium (0) (0.082 g), 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.1078 g) and potassium phosphate (0.545 g) were added, followed by the addition of 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.236 g) in DME (5 ml). The reaction mixture was heated at 70° C. with stirring for 4 hours. After cooling, the reaction mixture was diluted with MeOH (20 ml) and filtered. The filtrate was then placed on a 10 g SCX column. The column was washed with MeOH and eluted with 2NH3 in MeOH. All ammoniacal fractions were combined and the solvent was evaporated. The residue was purified on MDAP. The relevant fractions from MDAP were combined and the solvent stripped. The residue was then taken up in MeOH (2 ml) and treated with 1M HCl in ether (1 ml). The solvent was blown down to afford the title compound (E53) (112 mg). 1H NMR (methanol-d4) δ: 1.41 (6H, d, J=6.8 Hz), 2.23-2.44 (2H, m), 2.45 (3H, s), 3.04-3.18 (3H, m), 3.55-3.85 (9H, m), 4.35-4.49 (1H, m), 4.70-4.80 (1H, m), 7.62 (1H, dd, J=9.2 Hz), 8.14 (1H, s), 8.33 (1H, d, J=9.2 Hz). LCMS electrospray (+ve) 385 (MH+).
Method A
A mixture of 4-(4-methylimidazol-1-yl)iodobenzene and 4-(4-methylimidazol-1-yl)bromobenzene (may be prepared as described in Description 54) (1.58 g) was added to a solution of tris(dibenzylideneacetone) dipalladium(0) (0.152 g) and 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.196 g) in MeCN (10 ml). 1-(1-Methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (1 g in 10 ml MeCN) was then added followed by potassium phosphate (1.88 g). The mixture was heated to 80° C. for 5 h. After cooling, the mixture was diluted with MeOH, loaded onto 2×10 g SCX cartridges and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were evaporated and the residue was triturated with ether (×2) and crystallised from EtOH (×2). The residue was then dissolved in a minimum volume of MeOH and 1M HCl/diethylether (5 ml) added. The solvents were evaporated and the residue re-evaporated from acetone (×2) to afford the title compound (E55) as a white solid (0.21 g). LCMS electrospray (+ve) 382 (MH+). 1H NMR (DMSO-d6) δ 1.29 (6H, d, J=6.4 Hz), 2.07-2.12 (1H, m), 2.19-2.22 (1H, m), 2.33 (3H, s), 2.85-2.91 (1H, m), 3.00-3.12 (1H, m), 3.15-3.25 (1H, m), 3.30-3.75 (9H, m), 4.24 (1H, m), 4.51 (1H, m), 6.70 (2H, d, J=8.8 Hz), 7.52 (2H, d, J=8.8 Hz), 7.87 (1H, s), 9.46 (1H, s), 11.25 (1H, bs), 14.90 (1H, bs).
Method B
1-(1-Methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) (0.25 g in 2.5 ml MeCN) and potassium phosphate (0.47 g) were added to a stirred mixture of tris(dibenzylideneacetone) dipalladium(0) (0.076 g), 2-dicyclohexylphosphino-2′-(N,N-dimethylamino)-biphenyl (0.098 g), MeCN (2.5 ml) and a mixture of 4-(4-methylimidazol-1-yl)iodobenzene and 4-(4-methylimidazol-1-yl)bromobenzene (may be prepared as described in Description 54) (0.395 g). The mixture was heated to 80° C. for 5 h under argon. After cooling, the mixture was diluted with MeOH, loaded onto a 10 g SCX cartridge and eluted with MeOH (50 ml) followed by 2M NH3/MeOH (50 ml). The ammoniacal fractions were evaporated and the residue was washed with ether (×2) and crystallised from EtOH. The residue was then dissolved in a minimum volume of DCM and 0.5 ml 1M HCl/diethylether was added. The solvents were removed and the residue was evaporated from acetone (×2) to afford the title compound (E55) as a white solid (0.072 g). LCMS electrospray (+ve) 382 (MH+). 1H NMR (DMSO-d6) δ 1.29 (6H, d, J=6.4 Hz), 2.07-2.12 (1H, m), 2.19-2.22 (1H, m), 2.33 (3H, s), 2.85-2.91 (1H, m), 3.00-3.12 (1H, m), 3.15-3.25 (1H, m), 3.30-3.75 (9H, m), 4.24 (1H, m), 4.51 (1H, m), 6.70 (2H, d, J=8.8 Hz), 7.52 (2H, d, J=8.8 Hz), 7.87 (1H, s), 9.46 (1H, s), 11.25 (1H, bs), 14.90 (1H, bs).
1-(1-Methylethyl)-4-({(3S)-1-[4-(4-methyl-1H-imidazol-1-yl)phenyl]-3-pyrrolidinyl}carbonyl)piperazine dihydrochloride (may be prepared as described in Example 55) (0.145 g) was dissolved in MeOH and loaded onto a 10 g SCX cartridge. The cartridge was eluted with MeOH (60 ml) followed by 2M NH3/MeOH (60 ml). The ammoniacal fractions were evaporated to dryness. The residue was then dissolved in DCM and washed with an aqueous solution of K2CO3. The DCM layer was dried (MgSO4) and evaporated. The residue was then crystallised from ethanol to afford the title compound (E55A) as a crystalline white solid (0.04 g). LCMS: 382 (MH+) NMR (CDCl3): 1.06 (6H, d, J=6.4 Hz), 2.21-2.38 (5H, m), 2.50-2.57 (4H, m), 2.70-2.76 (1H, m), 3.35-3.59 (7H, m), 3.65-3.68 (2H, m), 6.58 (2H, d, J=8.8 Hz), 6.89 (1H, s), 7.19 (2H, d, J=8.8 Hz), 7.61 (1H, s).
The title compound (E56) was prepared using an analogous process to that described in Example 37 from 1-(1-methylethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 6) and 4-(2-methylimidazol-1-yl)iodobenzene (may be prepared as described in Example 8A of WO 96/11911), and the free base product was converted into the dihydrochloride salt. LCMS Electrospray (+ve) 382 (MH+). NMR (DMSO) 1.29 (6H, d, J=6.4 Hz), 2.04-2.29 (2H, m), 2.52 (3H, s), 2.93 (1H, m), 3.16 (1H, m), 3.21 (1H, m), 3.31-3.69 (9H, m), 4.24 (1H, m), 4.51 (1H, m), 6.70 (2H, d, J=8.8 Hz), 7.37 (2H, d, J=8.8 Hz), 7.72-7.75 (2H, m), 11.15 (1H, bs), 14.65 (1H, bs).
(2S)-1-(1-Methylethyl)-2-methyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 21) (0.12 g), 4-fluorobenzonitrile (0.121 g) and potassium carbonate (0.138 g) were heated in DMSO (1 ml) at 120° C. under argon for 2 h. After cooling, the mixture was divided into 2 equal portions and each portion applied to a 10 g SCX column. After initial elution (under gravity) with MeOH, the product was eluted with 2M NH3 in MeOH and the combined ammoniacal eluates were evaporated to give a gum, which was further purified by chromatography on silica gel eluting with 2M methanolic NH3/DCM (0 to 3.5% in 0.5% steps). The freebase was thereby obtained as a yellow gum (0.106 g). 1H NMR (CDCl3) 0.89 (3H, m), 1.06-1.16 (6H, m), 2.20-2.37 (3H, m), 2.50-2.86, 2.94-3.07 and 3.20-3.81 (3×m, total 11H), 4.21-4.31 (1H, m), 6.51 (2H, d, J=8 Hz), 7.46 (2H, d, J=8 Hz). This was dissolved in DCM (3 ml) and treated with 1M HCl in Et2O (3 ml). The solvents were blown off in a stream of argon and the residual material was dried in vacuo for 48 h at 40° C. to afford the title hydrochloride salt (E57) as a solid foam (0.111 g). LCMS electrospray (+ve) 341 (MH+).
Example 58 was prepared using an analogous process to that described in Example 51 from 5-bromo-2-(3-methyl-1,2,4-oxadiazol-5-yl)pyridine (may be prepared as described in Description 51) and 1-(cyclopropylmethyl)-4-[(3S)-pyrrolidin-3-ylcarbonyl]-hexahydro-1H-1,4-diazepine (may be prepared as described in Description 16). Example 59 was prepared using an analogous process to that described in Example 51 from 5-bromo-2-(3-methyl-1,2,4-oxadiazol-5-yl)pyridine (may be prepared as described in Description 51) and 1-(cyclopropylmethyl)-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 28). The free base products were converted into the corresponding hydrochloride salts in DCM (1 ml) with 1N ethereal HCl (2 ml) followed by evaporation of solvents. Examples displayed 1H NMR and mass spectral data consistent with structure.
The title compound (E60) was prepared from 1-ethyl-4-[(3S)-3-pyrrolidinylcarbonyl]piperazine (may be prepared as described in Description 57) (0.052 g) and 5-(4bromophenyl)-3-methyl-1,2,4-oxadiazole (may be prepared as described in Description D23) (0.058 g) using an analogous process to that described in Example 51. LCMS electrospray (+ve) 370 (MH+). 1H NMR δ: (methanol d4) δ: 1.35-1.45 (3H, t), 2.18-2.34 (2H, m), 2.36 (3H, s), 2.95-3.23 (6H, m), 3.45-3.75 (7, m), 4.35-4.49 (1H, m), 4.65-4.77 (1H, m), 6.70 (2H, d, J=8.0 Hz), 7.90 (2H, d, J=8.0 Hz).
Abbreviations
All publications, including but not limited to patents and patent applications, cited in this specification are herein incorporated by reference as if each individual publication were fully set forth.
Biological Data
A membrane preparation containing histamine H3 receptors may be prepared in accordance with the following procedures:
(i) Generation of Histamine H3 Cell Line
DNA encoding the human histamine H3 gene (Huvar, A. et al. (1999) Mol. Pharmacol. 55(6), 1101-1107) was cloned into a holding vector, pcDNA3.1 TOPO (InVitrogen) and its cDNA was isolated from this vector by restriction digestion of plasmid DNA with the enzymes BamH1 and Not-1 and ligated into the inducible expression vector pGene (InVitrogen) digested with the same enzymes. The GeneSwitch™ system (a system where in transgene expression is switched off in the absence of an inducer and switched on in the presence of an inducer) was performed as described in U.S. Pat. Nos. 5,364,791; 5,874,534; and 5,935,934. Ligated DNA was transformed into competent DH5α E. coli host bacterial cells and plated onto Luria Broth (LB) agar containing Zeocin™ (an antibiotic which allows the selection of cells expressing the sh ble gene which is present on pGene and pSwitch) at 50 μg ml−1. Colonies containing the re-ligated plasmid were identified by restriction analysis. DNA for transfection into mammalian cells was prepared from 250 ml cultures of the host bacterium containing the pGeneH3 plasmid and isolated using a DNA preparation kit (Qiagen Midi-Prep) as per manufacturers guidelines (Qiagen).
CHO K1 cells previously transfected with the pSwitch regulatory plasmid (InVitrogen) were seeded at 2×10e6 cells per T75 flask in Complete Medium, containing Hams F12 (GIBCOBRL, Life Technologies) medium supplemented with 10% v/v dialysed foetal bovine serum, L-glutamine, and hygromycin (100 μg ml−1), 24 hours prior to use. Plasmid DNA was transfected into the cells using Lipofectamine plus according to the manufacturers guidelines (InVitrogen). 48 hours post transfection cells were placed into complete medium supplemented with 500 μg ml−1 Zeocin™.
10-14 days post selection 10 nM Mifepristone (InVitrogen), was added to the culture medium to induce the expression of the receptor. 18 hours post induction cells were detached from the flask using ethylenediamine tetra-acetic acid (EDTA; 1:5000; InVitrogen), following several washes with phosphate buffered saline pH 7.4 and resuspended in Sorting Medium containing Minimum Essential Medium (MEM), without phenol red, and supplemented with Earles salts and 3% Foetal Clone II (Hyclone). Approximately 1×10e7 cells were examined for receptor expression by staining with a rabbit polyclonal antibody, 4a, raised against the N-terminal domain of the histamine H3 receptor, incubated on ice for 60 minutes, followed by two washes in sorting medium. Receptor bound antibody was detected by incubation of the cells for 60 minutes on ice with a goat anti rabbit antibody, conjugated with Alexa 488 fluorescence marker (Molecular Probes). Following two further washes with Sorting Medium, cells were filtered through a 50 μm Filcon™ (BD Biosciences) and then analysed on a FACS Vantage SE Flow Cytometer fitted with an Automatic Cell Deposition Unit. Control cells were non-induced cells treated in a similar manner. Positively stained cells were sorted as single cells into 96-well plates, containing Complete Medium containing 500 μg ml−1 Zeocin™ and allowed to expand before reanalysis for receptor expression via antibody and ligand binding studies. One clone, 3H3, was selected for membrane preparation.
(ii) Membrane Preparation from Cultured Cells
All steps of the protocol are carried out at 4° C. and with pre-cooled reagents. The cell pellet is resuspended in 10 volumes of homogenisation buffer (50 mM N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), 1 mM ethylenediamine tetra-acetic acid (EDTA), pH 7.4 with KOH, supplemented with 10e-6M leupeptin (acetyl-leucyl-leucyl-arginal; Sigma L2884), 25 μg/ml bacitracin (Sigma B0125), 1 mM phenylmethylsulfonyl fluoride (PMSF) and 2×10e-6M pepstain A (Sigma)). The cells are then homogenised by 2×15 second bursts in a 1 litre glass Waring blender, followed by centrifugation at 500 g for 20 minutes. The supernatant is then spun at 48,000 g for 30 minutes. The pellet is resuspended in homogenisation buffer (4× the volume of the original cell pellet) by vortexing for 5 seconds, followed by homogenisation in a Dounce homogeniser (10-15 strokes). At this point the preparation is aliquoted into polypropylene tubes and stored at −80° C.
A histamine H1 cell line may be generated in accordance with the following procedure:
(iii) Generation of Histamine H1 Cell Line
The human H1 receptor was cloned using known procedures described in the literature [Biochem. Biophys. Res. Commun. 1994, 201(2), 894]. Chinese hamster ovary cells stably expressing the human H1 receptor were generated according to known procedures described in the literature [Br. J. Pharmacol. 1996, 117(6), 1071].
Compounds of the invention may be tested for in vitro biological activity in accordance with the following assays:
(I) Histamine H3 Functional Antagonist Assay (Method A)
For each compound being assayed, in a solid white 384 well plate, is added:—
(a) 5 μl of test compound diluted to the required concentration in 10% DMSO (or 5 μl 10% DMSO as a control); and
(b) 30 μl bead/membrane/GDP mix prepared by mixing Wheat Germ Agglutinin Polystyrene LeadSeeker® (WGA PS LS) scintillation proximity assay (SPA) beads with membrane (prepared in accordance with the methodology described above) and diluting in assay buffer (20 mM N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES)+100 mM NaCl+10 mM MgCl2, pH7.4 NaOH) to give a final volume of 30 μl which contains 5 μg protein and 0.25 mg bead per well, incubating at 4° C. for 30 minutes on a roller and, just prior to addition to the plate, adding 10 μM final concentration of guanosine 5′ diphosphate (GDP) (Sigma; diluted in assay buffer).
The plates were then incubated at room temperature for 30 minutes on a shaker followed by addition of:
(c) 15 μl 0.38 nM [35S]-GTPγS (Amersham; Radioactivity concentration=37 MBq/ml; Specific activity=1160 Ci/mmol), histamine (at a concentration that results in the final assay concentration of histamine being EC80).
After 2-6 hours, the plate is centrifuged for 5 min at 1500 rpm and counted on a Viewlux counter using a 613/55 filter for 5 min/plate. Data is analysed using a 4-parameter logistical equation. Basal activity used as minimum i.e. histamine not added to well.
(II) Histamine H3 Functional Antagonist Assay (Method B)
For each compound being assayed, in a solid white 384 well plate, is added:—
(a) 0.5 μl of test compound diluted to the required concentration in DMSO (or 0.5 μl DMSO as a control);
(b) 30 μl bead/membrane/GDP mix prepared by mixing Wheat Germ Agglutinin Polystyrene LeadSeeker® (WGA PS LS) scintillation proximity assay (SPA) beads with membrane (prepared in accordance with the methodology described above) and diluting in assay buffer (20 mM N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES)+100 mM NaCl+10 mM MgCl2, pH7.4 NaOH) to give a final volume of 30 μl which contains 5 μg protein and 0.25 mg bead per well, incubating at room temperature for 60 minutes on a roller and, just prior to addition to the plate, adding 10 μM final concentration of guanosine 5′ diphosphate (GDP) (Sigma; diluted in assay buffer);
(c) 15 μl 0.38 nM [35S]-GTPγS (Amersham; Radioactivity concentration=37 MBq/ml; Specific activity=1160 Ci/mmol), histamine (at a concentration that results in the final assay concentration of histamine being EC80).
After 2-6 hours, the plate is centrifuged for 5 min at 1500 rpm and counted on a Viewlux counter using a 613/55 filter for 5 min/plate. Data is analysed using a 4-parameter logistical equation. Basal activity used as minimum i.e. histamine not added to well.
(III) Histamine H1 Functional Antagonist Assay
The histamine H1 cell line was seeded into non-coated black-walled clear bottom 384-well tissue culture plates in alpha minimum essential medium (Gibco/Invitrogen, cat no. 22561-021), supplemented with 10% dialysed foetal calf serum (Gibco/Invitrogen cat no. 12480-021) and 2 mM L-glutamine (Gibco/Invitrogen cat no 25030-024) and maintained overnight at 5% CO2, 37° C.
Excess medium was removed from each well to leave 10 μl. 30 μl loading dye (250 μM Brilliant Black, 2 μM Fluo-4 diluted in Tyrodes buffer+probenecid (145 mM NaCl, 2.5 mM KCl, 10 mM HEPES, 10 mM D-glucose, 1.2 mM MgCl2, 1.5 mM CaCl2, 2.5 mM probenecid, pH adjusted to 7.40 with NaOH 1.0 M)) was added to each well and the plates were incubated for 60 minutes at 5% CO2, 37° C.
10 μl of test compound, diluted to the required concentration in Tyrodes buffer+probenecid (or 10 μl Tyrodes buffer+probenecid as a control) was added to each well and the plate incubated for 30 min at 37° C., 5% CO2. The plates were then placed into a FLIPR™ (Molecular Devices, UK) to monitor cell fluorescence (λex=488 nm, λEM=540 nm) in the manner described in Sullivan et al. (In: Lambert DG (ed.), Calcium Signaling Protocols, New Jersey: Humana Press, 1999, 125-136) before and after the addition of 10 μl histamine at a concentration that results in the final assay concentration of histamine being EC80.
Functional antagonism is indicated by a suppression of histamine induced increase in fluorescence, as measured by the FLIPR™ system (Molecular Devices). By means of concentration effect curves, functional affinities are determined using standard pharmacological mathematical analysis.
Results
The compounds of Examples E17, E17A, E17B, E22 and E23 were tested in the histamine H3 functional antagonist assay (method A). The results are expressed as functional pKi (fpKi) values. A functional pKi is the negative logarithm of the antagonist equilibrium dissociation constant as determined in the H3 functional antagonist assay using membrane prepared from cultured H3 cells. The results given are averages of a number of experiments. These compounds exhibited antagonism >8.0 fpKi. More particularly, the compounds of Examples E17, E17A, E17B and E23 exhibited antagonism >9.5 fpKi.
The compounds of Examples E1-3, E4-5, E6, E7-16, E17, E18, E18A, E18B, E19, E19A, E19B, E20, E20A, E20B, E21, E21A, E21B, E23-31, E32A, E33-55 and E56-59 were tested in the histamine H3 functional antagonist assay (method B). Again, the results are expressed as functional pKi (fpKi) values and are averages of a number of experiments. These compounds exhibited antagonism >8.0 fKi. More particularly, the compounds of Examples E2, E3, E5, E6, E8-13, E17, E18, E18A, E18B, E19B, E20, E20B, E21, E21B, E24, E25, E27-30, E32A, E33-34, E36, E40, E41, E43, E47-49, E51-55 and E58 exhibited antagonism ≧9.5 fpKi.
The compounds of Examples E1-3, E4-5, E6, E7-17, E17A, E17B, E18, E18A, E18B, E19, E19A, E19B, E20, E20A, E20B, E21, E21A, E21B, E22-31, E32A, E33-55 and E56-59 were tested in the histamine H1 functional antagonist assay. The results are expressed as functional pKi (fpki) values and are averages of a number of experiments. The functional pKi may be derived from the negative logarithm of the pIC50 (concentration producing 50% inhibition) in the H1 functional antagonist assay according to the Cheng-Prusoff equation (Cheng, Y. C. and Prusoff, W. H., 1973, Biochem. Pharmacol. 22, 3099-3108.). All compounds tested exhibited antagonism <6.5 fpKi.
| Number | Date | Country | Kind |
|---|---|---|---|
| 0423005.8 | Oct 2004 | GB | national |
| 0508441.3 | Apr 2005 | GB | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP05/11371 | 10/13/2005 | WO | 4/10/2007 |
