Pyrrolo [1,2-a] thieno [3,2-f] (1,4) diazepines

The present invention relates to novel 4H-pyrrolo [1,2-a]thieno[3,2-f][1,4]diazepine, to their process of preparation and to the pharmaceutical compositions containing them.
Some 5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine compounds are described in the literature. (Heterocycles, 1979, Vol. 12, No. 8, pp. 1009-1011 and Tetrahedron Letters, 1979, No. 7, pp. 643-644). It is also known that some 6H-pyrrolo[1,2a]thieno[3,2-f][1,4]diazepine compounds might have antineoplastic properties (C. R. Acad. Sc. Paris, 1978, 287, pp. 117-120). 4-Phenyl-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine compounds are described in J. Heter. Chem., 1977, 14, No. 2, pp. 235-240.
The pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepines of the Applicant are compounds of novel structure which possess advantageous pharmacological properties.
These compounds are endowed with exceptional antihypoxic properties, which are very advantageous in the treatment of cerebral aging, stroke and ischemic syndromes of any localization, acute, transient or progressive, through exerting their pharmacological properties with respect to the oxygen deficiency which accompanies these mishaps.
They are also potent CCK (cholecystokinin) antagonists. Diazepines which are cholecystokinin antagonists and which bind specifically to receptors for the latter, thereby enabling their use to be envisaged in the treatment of disorders of the central nervous system, stomach, intestine, pancreas or gall bladder and other CCK-dependent disorders, have already been described in European Patent 167,919. The compounds of the invention, while displaying activities equal to those of the most active compounds of the abovementioned European patent, are distinctly less toxic, so that their therapeutic index is better.
The compounds of the Applicant are also extremely advantageous by virtue of their metabolic effects, since they possess substantial hypoglycemic, hypocholesterolemic and hypotriglyceridemic properties, being, for example, much more active than clofibrate.
More specifically, the invention relates to the compounds of general formula (I): ##STR5## in which: R.sub.1 represents a radical of general formula (Z.sub.0), (Z.sub.1), (Z.sub.2), (Z.sub.3) or (Z.sub.4) ##STR6## in which formulae: R.sub.3 represents a hydrogen atom or a linear or branched alkyl radical having 1 to 6 carbon atoms, saturated or containing a double bond,
R.sub.9, R.sub.10 and R.sub.11, which may be identical or different, each represent a hydrogen atom, a halogen atom or an alkyl radical having 1 to 6 carbon atoms,
R.sub.12 represents a hydrogen atom or, with R.sub.2 and the nitrogen atom to which they are attached, forms a radical of general formula (W): ##STR7## R.sub.2 represents a methylene radical, a hydroxymethylene radical, a carbonyl radical or a radical of general formula (Y.sub.1), (Y.sub.2), (Y.sub.3) or (Y.sub.4): ##STR8## or, with R.sub.12 and the nitrogen atom to which they are attached, forms a radical of general formula (W): ##STR9## in which formulae: R.sub.4 represents an oxygen or sulfur atom, a carbonyl radical or a radical of general formula (X.sub.1), (X.sub.2), (X.sub.3) or (X.sub.4): ##STR10## n is between 0 and 4 inclusive, R.sub.5 represents a hydrogen atom, a linear or branched alkyl chain having 1 to 10 carbon atoms, optionally interrupted by one or more oxygen or sulfur atoms, a phenyl or benzoyl radical or an aralkyl radical having 7 to 11 carbon atoms (optionally substituted on the aromatic ring with one or more halogen atoms, linear or branched alkyl radicals having 1 to 6 carbon atoms, nitro radicals or linear or branched alkoxy radicals having 1 to 6 carbon atoms), a linear or branched carboxyalkyl radical having 2 to 7 carbon atoms, a linear or branched alkoxycarbonylalkyl radical having 3 to 10 carbon atoms, a linear or branched alkoxycarbonyl radical having 2 to 7 carbon atoms, a linear or branched carbamoylalkyl radical having 2 to 7 carbon atoms, a cycloalkyl radical having 3 to 7 carbon atoms or an unsaturated 5- to 7-membered ring-system comprising at least one hetero atom selected from nitrogen, sulfur and oxygen, a pyridylcarbonyl or pyrimidinylcarbonyl radical, a clofibroyl radical or a 6-hydroxy-2,5,7,8-tetramethylchroman-2-carbonyl radical,
R.sub.6 represents a hydrogen atom or a linear or branched alkyl radical having 1 to 6 carbon atoms,
R.sub.7 and R.sub.8, which may be identical or different, each represent a hydrogen atom, a linear or branched alkyl radical having 1 to 6 carbon atoms, a phenyl radical or a phenylalkyl radical having 7 to 9 carbon atoms (optionally substituted on the aromatic ring with one or more halogen atoms or alkyl or alkoxy groups having 1 to 6 carbon atoms), or, together with the nitrogen atom to which they are attached, form a saturated or unsaturated 5- to 7-membered ring-system comprising 1 to 2 hetero atoms selected from nitrogen, oxygen and sulfur, optionally substituted with an alkylcarbonyl or alkoxycarbonyl group having 2 to 5 carbon atoms,
R.sub.13 represents a hydrogen atom, a linear or branched alkylcarbonyl radical having 2 to 6 carbon atoms or a benzoyl radical,
with the provisos that:
when R.sub.1 represents a radical of general formula (Z.sub.3), then R.sub.2 cannot represent a methylene radical,
when R.sub.1 represents a radical of general formula (Z.sub.0) and R.sub.12 represents a hydrogen atom, then R.sub.2 cannot represent the following radicals: ##STR11## when R.sub.1 represents a radical of general formula (Z.sub.0), R.sub.12 represents a hydrogen atom and R.sub.2 represents a radical of general formula (Y.sub.1) with n=1 and R.sub.4 representing a carbonyl radical, then R.sub.5 cannot represent a methyl, isopropyl or phenyl radical,
when R.sub.1 represents a radical of general formula (Z.sub.0) and R.sub.12 represents a hydrogen atom, then R.sub.2 cannot represent a carbonyl radical.
The present invention also encompasses a process for obtaining the compounds of general formula (I), wherein:
EITHER
3-cyano-2-(2-formyl-1-pyrrolyl)thiophene, the compound of formula (II): ##STR12## is reacted
either
with a methyl ketone of general formula (III): ##STR13## in which R.sub.5 has the same meaning as in the compounds of general formula (I), in the presence of a strong inorganic base and hydrogen peroxide, so as to obtain the compounds of general formula (I.sub.A): ##STR14## in which R.sub.5 has the same meaning as in the compounds of general formula (I),
which are then:
either
subjected to the action of sodium borohydride, in solution in an alcoholic solvent, so as to obtain the compounds of general formula (I.sub.B): ##STR15## in which R.sub.5 has the same meaning as in the compounds of general formula (I), which can optionally be subjected to the action of phosgene, in an aromatic organic solvent, in the heated state, to form the compounds of general formula (I.sub.C): ##STR16## in which R.sub.5 has the same meaning as in the compounds of general formula (I),
or be reacted with a compound of general formula (XI):
R.sub.13 --Cl (XI)
in which R.sub.13 has the same meaning as in the compounds of general formula (I), so as to obtain the compounds of general formula (I.sub.H): ##STR17## in which R.sub.5 and R.sub.13 have the same meaning as in the compounds of general formula (I),
or
condensed with a hydroxylamine derivative of general formula (IV):
H.sub.2 N--O--R.sub.6 (IV)
in which R.sub.6 has the same meaning as in the compounds of general formula (I), to form the compounds of general formula (I.sub.D): ##STR18## in which R.sub.5 and R.sub.6 have the same meaning as in the compounds of general formula (I),
or
condensed with a hydrazine compound of general formula (XII):
H.sub.2 N--NH--R.sub.6 (XII)
in which R.sub.6 has the same meaning as in the compounds of general formula (I), so as to obtain the compounds of general formula (I.sub.N): ##STR19## in which R.sub.5 and R.sub.6 have the same meaning as in the compounds of general formula (I),
or
with a primary alcohol or general formula (V):
R.sub.3 OH (V)
in which R.sub.3 has the same meaning as in the compounds of general formula (I),
in the presence of a strong inorganic base and at a temperature of between 30.degree. and 80.degree. C., so as to obtain the compounds of general formula (I.sub.E): ##STR20## in which R.sub.3 has the same meaning as in the compounds of general formula (I), which is then subjected to the action of potassium permanganate, at room temperature, to form the compounds of general formula (I.sub.F): ##STR21## in which R.sub.3 has the same meaning as in the compounds of general formula (I), which can optionally be subjected to the action of sodium borohydride to obtain the compounds of general formula (I.sub.G): ##STR22## in which R.sub.3 has the same meaning as in the compounds of general formula (I),
OR
3-carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene, the compound of formula (VI): ##STR23## is subjected
either
to the action of an amine of general formula (VII): ##STR24## in which R.sub.7 and R.sub.8 have the same meaning as in the compounds of general formula (I), so as to obtain the compounds of general formula (I.sub.H): ##STR25## in which R.sub.7 and R.sub.8 have the same meaning as in the compounds of general formula (I), which can optionally be subjected, in the case where R.sub.7 and R.sub.8, together with the nitrogen atom to which they are attached, form a morpholino radical, to the action of sodium borohydride, to obtain the compound of general formula (I) in which R.sub.2 represents a methylene radical and R.sub.1 a radical of general formula (Z.sub.0) with R.sub.12 =H,
or
to the action of triethylamine in the presence of water and at room temperature to form the compound of general formula (I) in which R.sub.2 represents a hydroxymethylene radical and R.sub.1 a radical of formula (Z.sub.0) with R.sub.12 =H,
which is then:
either
reacted with an amine of general formula (VII), to form the compounds of general formula (I.sub.H),
or
condensed with a compound of general formula (VIII): ##STR26## in which R.sub.6 has the same meaning as in the compounds of general formula (I), in the presence of triethylamine, to form the compounds of general formula (I.sub.I): ##STR27## in which R.sub.6 has the same meaning as in the compounds of general formula (I),
or
reacted with an alcohol of general formula (IX):
R.sub.5 OH (IX)
in which R.sub.5 has the same meaning as in the compounds of general formula (I), in the heated state, to obtain the compounds of general formula (I.sub.J): ##STR28## in which R.sub.5 has the same meaning as in the compounds of general formula (I):
or
to the action of a compound of general formula (X): ##STR29## in which R.sub.5 and R.sub.6 have the same meaning as in the compounds of general formula (I), to form the compounds of general formula (I.sub.K): ##STR30## in which R.sub.5 and R.sub.6 have the same meaning as in the compounds of general formula (I),
or
to the action of an alcohol of general formula (IX), to form the compounds of general formula (I.sub.J),
or
to the action of a thiol of general formula (XI):
R.sub.5 SH (XI)
in which R.sub.5 has the same meaning as in the compounds of general formula (I), at room temperature, to form the compounds of general formula (I.sub.L): ##STR31## in which R.sub.5 has the same meaning as in the compounds of general formula (I), which can optionally be subjected, in the case where R.sub.5 represents a carboxyalkyl radical, to the action of triethylamine and a chloroformate and then to the action of gaseous ammonia to form the corresponding amides.
The compounds of general formulae (I.sub.A) to (I.sub.N) form part of the invention and are comprised within the compounds of general formula (I).
3-Cyano-2-(2-formyl-1-pyrrolyl)thiophene, the compound of formula (II), is an already known compound (Heterocycles, 1986, Vol. 24, (3), pp. 575-578).
The condensation of the compound of general formula (II) with the compounds of general formula (III) is performed in an aqueous-alcoholic mixture, in the heated state, in the presence of a strong inorganic base and hydrogen peroxide.
The reduction of the compounds of general formula (I.sub.A) with sodium borohydride, with leads to the production of the compounds of general formula (I.sub.B), is carried out in a low molecular weight primary alcohol at room temperature.
To obtain the compounds of general formula (I.sub.C), the cyclization of the compounds of general formula (I.sub.B) is carried out in the heated state, with phosgene, in an aromatic organic solvent such as, for example, toluene.
The compounds of general formula (I.sub.D) are obtained from the compounds of general formula (I.sub.A). The latter are dissolved in a low molecular weight alcohol. The compounds of general formula (IV) are then added in the presence of sodium acetate and the mixture is heated to reflux.
3-Carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene, the compound of formula (VI), was prepared from the compound of formula (II) according to conventional methods.
The reaction of the compound of formula (VI) with the amines of general formula (VII) is performed in water at room temperature when the amines are present in excess in the reaction medium (10 times the requisite molar quantity), or in the heated state in acetonitrile when the quantities of the compounds (VI) and (VII) are equimolar.
To obtain the compounds of general formula (I.sub.K), the reaction of the compounds of general formula (X) with the compound of formula (VI) is performed in water at a temperature of between 50.degree. and 70.degree. C. The end of the reaction is carried out at room temperature.
The compounds according to the invention, as well as their salts, are endowed with highly advantageous pharmacological properties. In effect, pharmacological tests in vivo showed that the compounds of the present invention exert a potent antihypoxic effect in animals.
During aging or following a stroke, increased frailty and cell vulnerability are physiopathological components of importance in the search for new treatments directed towards protecting the brain placed in the position of inability to respond to any further stress emanating from its environment.
Such a stress may be reproduced in the form of a defective oxygen supply, and for this reason, in terms of their consequences, there is a close analogy between hypoxia and cerebral aging.
The compounds of the present invention were tested for their capacity to prolong survival of the cerebral tissue during acute hypoxia in mice. The results of the tests demonstrated that the compounds of the invention have a very potent antihypoxic protective effect, and thus confirmed the great value of their use in therapy.
By clearly counteracting brain death during insufficiency of the oxygen supply, the compounds of the present invention exert a pronounced antihypoxic effect and are hence useful in the case of ischemic syndromes of any localization, acute, transient or progressive, since they exert their pharmacological properties with respect to the oxygen deficiency which accompanies these mishaps. Their pharmacological properties enable them to be applied in the correction of disorders linked to hypoxemia, for example during cerebral aging.
The affinity of the compounds of the invention for cholecystokinin receptors was studied by determining the median inhibitory concentration (IC.sub.50) for the binding of iodine-125-labeled cholecystokinin to the receptors of rat pancreas plasma membranes and of guinea pig brain membranes. For the majority of the compounds of the invention, it was found that the ratio of the IC.sub.50 for the brain receptors to the IC.sub.50 for the pancreatic receptors is very high. This demonstrates that the compounds of the invention display better selectivity for the peripheral cholecystokinin receptors, and permit better treatment of the disorders dependent thereon, with fewer side effects.
The compounds of the invention are hence seen to be especially useful in human or veterinary therapy in the treatment of cholecystokinin-dependent stomach, intestine, pancreas and gall bladder disorders, such as, for example, pancreatitis, disorders of gastric or gall bladder motor function, ulcers and irritable colon syndrome. They also find their application in the treatment of pain, where appropriate of disorders dependent on the interaction of cholecystokinin with neuromediators of the central nervous system, such as, for example, neuroleptic disorders, Parkinson's disease, psychosis and tardive dyskinesia. The compounds of the invention are also useful for regulating the appetite.
The compounds of the invention also possess exceptional metabolic properties, being strongly hypolipemic, hypocholesterolemic and hypotriglyceridemic.
When given for 14 days to rats subjected to a hypercholesterolemic diet, they proved to be much more active (at least 30-fold) than clofibrate with respect to the lowering of the plasma triglyceride, cholesterol and HDL (high density lipoprotein)-cholesterol levels.
The toxicity was evaluated in male mice. The LD.sub.50 of the compounds of the invention is greater than 1,500 mg/Kg (administered i.p.).
The compounds of general formula (I), as well as their addition salts with a pharmaceutically acceptable inorganic or organic acid such as, for example, hydrochloric, methanesulfonic, citric and maleic acids, may be made into pharmaceutical preparations according to generally known processes, such as, for example, into tablets, hard gelatin capsules, dragees, solutions for oral administration, injectable solutions, suspensions for oral administration, emulsions and suppositories.
Apart from non-toxic and pharmaceutically acceptable inert excipients such as, for example, distilled water, glucose, lactose, starch, talc, vegetable oils, ethylene glycol, and the like, these preparations can also contain preservatives, stabilizers, wetting agents, emulsifiers, and the like.
The compositions thereby obtained generally take the form of measured doses, and can contain, depending on the conditions treated and the patient's age and sex, from 0.1 to 100 mg of active principle. Depending on the circumstances, they may be administered orally, rectally or parenterally, at a dose of 0.1 to 100 mg from one to several times a day.
The examples which follow illustrate the invention and in no way limit the latter.
The .sup.1 H NMR spectrometry characteristics are collated in Tables I to XIII.

EXAMPLE 1
5,6-dihydro-4-oxo-6-(2-oxo-1-pentyl)-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 12 g (0.059 mole) of 3-cyano-2-(2-formyl-1-pyrrolyl)thiophene in a mixture of 200 ml of 2-pentanone, 200 ml of 6N sodium hydroxide and 15 ml of 33% hydrogen peroxide is heated to reflux for one hour. The ethanol and 2-pentanone are then removed under vacuum and the remainder of the reaction mixture is poured into 200 ml of cold water. The precipitate formed is drained, washed with water, dried and recrystallized.
Yield: 68%.
Melting point: 186.degree. C. (Ethyl ether/acetone), yellow crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 62.49 5.59 9.72 11.10Found 62.48 5.50 9.72 11.23______________________________________
IR spectrum (KBr): NH bands at 3275 and 3170 cm.sup.-1, CH bands at 3050, 2950, 2920 and 2870 cm.sup.-1, C.dbd.O bands at 1705 (ketone) and 1640 cm.sup.-1 (lactam), main bands at 1470, 1440, 1325, 1150, 1095, 795, 705 and 685 cm.sup.-1.
EXAMPLE 2
6-(2-cyclopropyl-2-oxo-1-ethyl)-5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 1, but using cyclopropyl methyl ketone instead of 2-pentanone.
Yield: 57%.
Melting point: 200.degree. C. (Ethyl ether/acetone), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 62.91 4.92 9.78 11.19Found 62.87 4.93 9.81 11.05______________________________________
IR spectrum (KBr): NH bands at 3270 and 3180 cm.sup.-1, CH bands at 3120, 3090, 3050 and 2890 cm.sup.-1, CO bands at 1685 and 1640 cm.sup.-1, main bands at 1530, 1475, 1440, 1395, 1330, 1090, 910 and 735 cm.sup.-1.
EXAMPLE 3
5,6-dihydro-4-oxo-6-[2-oxo-2-(phenethyl)ethyl]-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 1, but using benzyl acetone as a ketone.
Yield: 19%.
Melting point: 172.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 68.55 5.18 7.99 9.15Found 68.22 5.36 7.90 8.85______________________________________
IR spectrum (KBr): NH bands at 3270 and 3170 cm.sup.-1, CH bands at 3050, 3020, 2920 and 2880 cm.sup.-1, CO bands at 1710 and 1645 cm.sup.-1, main bands at 1485, 1440, 1330, 1190, 1100 and 705 cm.sup.-1.
EXAMPLE 4
5,6-dihydro-6-(4-methylphenacyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 1, but using 4-methylacetophenone instead of 2-pentanone.
Yield: 44%.
Melting point: 190.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 67.84 4.79 8.33 9.53Found 68.39 5.01 8.13 8.96______________________________________
IR spectrum (KBr): NH band at 3320 cm.sup.-1, CH bands at 3080, 3060, 3040 and 2920 cm.sup.-1, CO band at 1670 cm.sup.-1, main bands at 1485, 1435, 1325, 810, 705 and 685 cm.sup.-1.
EXAMPLE 5
5,6-dihydro-6-(2-methylphenacyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 1, but using ortho-acetyltoluene.
Yield: 44%.
Melting point: 180.degree. C. (Ethyl ether), gray crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 67.84 4.79 8.33 9.53Found 67.72 4.82 8.44 9.43______________________________________
IR spectrum (KBr): NH bands at 3270 and 3160 cm.sup.-1, CH bands at 3040, 2920 and 2890 cm.sup.-1, CO bands at 1680 and 1650 cm.sup.-1, main bands at 1530, 1480, 1435, 1330, 1210, 1145, 985, 810, 765 and 720 cm.sup.-1.
EXAMPLE 6
5,6-dihydro-6-(2,5-dimethoxyphenacyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in the previous example, but using 2,5-dimethoxyacetophenone.
Yield: 19%.
Melting point: 180.degree. C. (Acetonitrile), white flocculent product.
______________________________________Elemental analysis: C % H % N %______________________________________Theory 62.81 4.74 7.32Found 62.75 4.76 7.34______________________________________
IR spectrum (KBr): NH bands at 3260 and 3190 cm.sup.-1, CH bands at 3050, 2930, 2900 and 2830 cm.sup.-1, C.dbd.O band at 1675 cm.sup.-1, main bands at 1490, 1410, 1220, 1035, 810 and 700 cm.sup.-1.
EXAMPLE 7
6-(4-chlorophenacyl)-5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 1, but using 4-chloroacetophenone.
Yield: 24%.
Melting point: 224.degree. C. (Ethanol), beige crystals.
______________________________________Elemental analysis: C % H % N % Cl %______________________________________Theory 60.58 3.67 7.85 9.93Found 61.14 3.66 7.53 10.49______________________________________
IR spectrum (KBr): NH band at 3320 cm.sup.-1, CH bands at 3080 and 2910 cm.sup.-1, C.dbd.O bands at 1740 cm.sup.-1 (acetone) and 1635 cm.sup.-1 (lactam), main bands at 1580, 1480, 1430, 1320, 1210, 1090, 990 and 705 cm.sup.-1.
EXAMPLE 8
5,6-dihydro-4-oxo-6-[2-oxo-2-(2-thienyl)ethyl]-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in the previous example, but using 2-acetylthiophene.
Yield: 20%.
Melting point: 186.degree. C. (Isopropanol), beige crystals.
______________________________________Elemental analysis: C % H % S %______________________________________Theory 58.52 3.68 19.52Found 57.91 3.91 18.90______________________________________
IR spectrum (KBr): NH band at 3320 cm.sup.-1, CH bands at 3080, 2960 and 2920 cm.sup.-1, CO bands at 1655 cm.sup.-1 and 1640 cm.sup.-1, main bands at 1530, 1485, 1440, 1325, 1220, 1140, 860, 720 and 710 cm.sup.-1.
EXAMPLE 9
5,6-dihydro-6-(4-fluorophenacyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 1, but using 4-fluoroacetophenone.
Yield: 21%.
Melting point: 170.degree. C. (Ethyl ether), beige crystals.
______________________________________Elemental analysis: C % H % S % F %______________________________________Theory 63.51 3.84 9.41 5.58Found 63.61 3.66 8.88 5.40______________________________________
IR spectrum (KBr): NH band at 3320 cm.sup.-1, C.dbd.O bands at 1670 cm.sup.-1 (ketone) and 1640 cm.sup.-1 (lactam), main bands at 1595 1485, 1440, 1330, 1215, 1000, 840 and 710 cm.sup.-1.
EXAMPLE 10
5,6-dihydro-6-(2-furyl-2-oxoethyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in the previous example, but using 2-acetylfuran.
Yield: 19.6%.
______________________________________Elemental analysis: C % H % S %______________________________________Theory 61.53 3.87 10.26Found 61.29 3.69 10.41______________________________________
IR spectrum (KBr): NH band at 3180 cm.sup.-1, CH bands at 3050 and 2920 cm.sup.-1, C.dbd.O bands at 1670 cm.sup.-1 (ketone) and 1640 cm.sup.-1 (lactam), main bands at 1485, 1460, 1435, 1330, 1145, 995 and 725 cm.sup.-1.
EXAMPLE 11
5,6-dihydro-6-(3,3-dimethyl-2-oxo-1-butyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 1, starting with pinacolone.
After removal of the ethanol, the remainder of the reaction mixture is poured into 100 ml of water. The oily precipitate formed is extracted with ethyl ether. The ether phase is washed with water, dried over magnesium sulfate and taken down under vacuum. The pinacolone is then removed from the residue by washing with a petroleum ether/ethyl ether (50:50 v/v) mixture. The insoluble solid is drained, washed with water, dried and recrystallized.
Yield: 26%.
Melting point: 170.degree. C. (Ethyl ether), beige crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 63.55 5.99 9.26 10.60Found 62.20 5.72 8.80 10.41______________________________________
IR spectrum (KBr): NH bands at 3260 and 3170 cm.sup.-1, CH bands at 3110, 3060, 2980 and 2900 cm.sup.-1, C.dbd.O bands at 1700 cm.sup.-1 (ketone) and 1735 cm.sup.-1 (lactam), main bands at 1485 1430, 1330, 1195, 1090, 830, 735 and 720 cm.sup.-1.
EXAMPLE 12
5,6-dihydro-6-(2-hydroxypropyl)-4-oxo-4H-pyrrolo-[1,2-a]thieno[3,2-f][1,4]diazepine
1.46 g of sodium borohydride are added in small portions to a solution of 2.5 g of 6-acetonyl-5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine in 200 ml of methanol, and the reaction mixture is then stirred at room temperature for 3 hours. The methanol is then removed under vacuum and the residual solid ground in 200 ml of water. The precipitate obtained is drained, washed with water, dried and recrystallized.
Yield: 80%.
Melting point: 171.degree. C. (Ethyl ether), white crystal.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.47 5.34 10.67 12.20Found 59.42 5.28 10.72 12.08______________________________________
IR spectrum (KBr): OH band at 3450 cm.sup.-1, NH bands at 3260 and 3190 cm.sup.-1, CH bands at 3040, 2960, 2920 and 2880 cm.sup.-1, CO band at 1630 cm.sup.-1, main bands at 1535, 1485, 1440, 1330, 1085, 885, 700 and 680 cm.sup.-1.
EXAMPLE 13
5,6-dihydro-4-oxo-6-(2-hydroxy-1-pentyl)-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound described in Example 1 and according to the process described in Example 12.
Yield: 80%.
Melting point: 188.degree. C. (Isopropanol), yellow crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 62.04 6.24 9.64 11.04Found 62.10 6.18 9.55 10.89______________________________________
IR spectrum (KBr): OH band at 3440 cm.sup.-1, NH bands at 3250 and 3170 cm.sup.-1, CH bands at 3030, 2950, 2930, 2900 and 2860 cm.sup.-1, CO band at 1630 cm.sup.-1, main bands at 1530, 1480, 1440, 1335, 1090, 1025, 805, 705 and 690 cm.sup.-1.
EXAMPLE 14
6-(2-cyclopropyl-2-hydroxyethyl)-5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound of Example 2 according to the process described in Example 12.
Yield: 80%.
Melting point: 222.degree. C. (Isopropanol), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 62.47 5.59 9.71 11.11Found 61.82 5.72 9.26 10.51______________________________________
IR spectrum (KBr): OH band at 3450 cm.sup.-1, NH bands at 3260 and 3180 cm.sup.-1, CH bands at 3080, 3000, 2950 and 2880 cm.sup.-1, CO band at 1625 cm.sup.-1, main bands at 1530, 1485, 1440, 1330, 1300, 1095, 830, 800, 710 and 690 cm.sup.-1.
EXAMPLE 15
5,6-dihydro-6-(2-hydroxyphenethyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from 5,6-dihydro-4-oxo-6-phenacyl-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]-diazepine (Tetrahedron Letters, 1979, No. 7, pp. 643-644) according to the process described in Example 12.
Yield: 79%.
Melting point: 190.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 66.65 4.97 8.64 9.88Found 66.56 4.89 8.52 9.68______________________________________
IR spectrum (KBr): OH band at 3450 cm.sup.-1, NH bands at 3250 and 3180 cm.sup.-1, CH bands at 3030, 2955 and 2900 cm.sup.-1, CO band at 1635 cm.sup.-1, main bands at 1485, 1440, 1335, 1060 and 710 cm.sup.-1.
EXAMPLE 16
5,6-dihydro-6-[2-hydroxy-2-(4-methylphenyl)ethyl]-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound described in Example 4 and according to the process described in Example 12.
Yield: 71%.
Melting point: 182.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 67.43 5.36 8.27 9.47Found 67.30 5.44 8.16 9.33______________________________________
IR spectrum (KBr): OH band at 3420 cm.sup.-1, NH bands at 3250 and 3170 cm.sup.-1, CH bands at 3100, 3020, 2950 and 2920 cm.sup.-1, CO band at 1635 cm.sup.-1, main bands at 1490, 1440, 1335, 1095, 1060, 830, 735 and 705 cm.sup.-1.
EXAMPLE 17
5,6-dihydro-6-(2-hydroxy-3-methyl-1-butyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from 5,6-dihydro-2-oxo-3-methyl-1-butyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine according to the process described in Example 12.
Yield: 83%.
Melting point: 194.degree. C. (Acetone), beige crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 62.04 6.24 9.64 11.04Found 62.00 6.17 9.64 10.97______________________________________
IR spectrum (KBr): OH band at 3460 cm.sup.-1, NH bands at 3250 and 3170 cm.sup.-1, CH bands at 3020, 2950, 2930 and 2870 cm.sup.-1, CO band at 1625 cm.sup.-1, main bands at 1530, 1490, 1445, 1330, 1100, 1055 and 710 cm.sup.-1.
EXAMPLE 18
5,6-dihydro-6-[2-hydroxy-2-(2,5-dimethoxyphenyl)ethyl]-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound of Example 6 according to the process described in Example 12.
Yield: 65%.
Melting point: 193.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 62.48 5.24 7.28 8.33Found 61.53 5.25 7.37 8.55______________________________________
IR spectrum (KBr): OH band at 3420 cm.sup.-1, NH bands at 3260 and 3180 cm.sup.-1, CH bands at 3080, 3000, 2960, 2900 and 2820 cm.sup.-1, CO band at 1625 cm.sup.-, main bands at 1530, 1490, 1440, 1330, 1275, 1215, 1100, 1025, 800, 790 and 720 cm.sup.-1.
EXAMPLE 19
4,6-dioxo-8-methyl-4,8,9,10-tetrahydro[1,3]oxazino[4,3-c]pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
3 ml of a 20% solution of phosgene in toluene is added to a solution of 0.7 g of the compound obtained in Example 12 in 150 ml of toluene, and the reaction mixture is then heated to reflux for one hour. After cooling, the excess phosgene is removed by bubbling a stream of nitrogen through the reaction medium. The toluene is then removed under vacuum and the residue ground in 200 ml of water. The precipitate is drained, washed with water, dried and recrystallized.
EXAMPLE 20
4,6-dioxo-8-propyl-4,8,9,10-tetrahydro[1,3]oxazino[4,3-c]pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was obtained from the compound of Example 13 and according to the process described in the previous example.
Yield: 89%.
Melting point: 162.degree. C. (Ethyl ether), white crystals.
IR spectrum (KBr): CH bands at 3020, 2960, and 2880 cm.sup.-1, C.dbd.O bands at 1745 and 1665 cm.sup.-1, main bands at 1385, 1280, 1195, 1160, 840 and 700 cm.sup.-1.
EXAMPLE 21
4,6-dioxo-8-(4-methylphenyl)-4,8,9,10-tetrahydro[1,3]oxazino[4,3-c]pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was obtained from the compound of Example 16 and according to the process described above.
Yield: 78%.
Melting point: 240.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 65.89 4.39 7.68 8.78Found 65.72 4.33 7.61 8.72______________________________________
IR spectrum (KBr): CH bands at 3030 and 2920 cm.sup.-1, C.dbd.O bands at 1700 and 1625 cm.sup.-1, main bands at 1490, 1440, 1330 and 715 cm.sup.-1.
EXAMPLE 22
5,6-dihydro-6-(2-hydroxyimino-1-propyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 4.2 g of hydroxylamine hydrochloride and 4.92 g of sodium acetate in 20 ml of water is added to a solution of 4 g of 6-acetonyl-5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine in 500 ml of ethanol.
After the reaction medium has been heated to reflux for one hour, the ethanol is removed under vacuum. The residual solid is ground with 300 ml of water, drained, washed with water, dried and recrystallized.
Yield: 85%.
Melting point: 178.degree. C. (Ethanol), white powder.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.11 5.22 14.52 11.08Found 57.99 5.16 14.47 11.10______________________________________
IR spectrum (KBr): OH band at 3280 cm.sup.-1, NH band at 3200 cm.sup.-1, CH bands at 3100 and 2860 cm.sup.-1, CO band at 1625 cm.sup.-1, main bands at 1540, 1495, 1440, 1325, 1090, 930, 890 and 715 cm.sup.-1.
EXAMPLE 23
5,6-dihydro-6-(2-hydroxyimino-3-methylbutyl)-4-oxo-4H-pyrrolo[1,2-a]thiene[3,2-f][1,4]diazepine
A solution of 2.80 g of hydroxylamine hydrochloride and 3.3 g of sodium acetate in 15 ml of water is added to a solution of 3 g of 5,6-dihydro-6-(3-methyl-2-oxo-1-butyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine in 300 ml of ethanol.
The reaction mixture is heated to reflux for one hour and the ethanol is then removed under vacuum. The residual solid is ground in 250 ml of water, drained, washed with water, dried and recrystallized.
Yield: 92%.
Melting point: 188.degree. C. (Isopropanol), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.38 5.64 13.85 10.56Found 59.46 5.69 13.78 10.46______________________________________
IR spectrum (KBr): OH band at 3270-3130 cm.sup.-1, NH band CH bands at 3100, 2960 and 2870 cm.sup.-1, CO band at 1635 cm.sup.-1, main bands at 1535, 1490, 1440, 1325, 1145, 1100, 950, 900, 785, 730 and 715 cm.sup.-1.
EXAMPLE 24
5,6-dihydro-6-(2-cyclopropyl-2-hydroxyimino-1-ethyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 4.9 g of hydroxylamine hydrochloride and 5.8 g of sodium acetate in 20 ml of water is added to a solution of 5 g of the compound of Example 2 in 500 ml of ethanol.
After the mixture has been heated to reflux for one hour, the ethanol is removed under vacuum. The residual solid is ground with 300 ml of water and the precipitate formed is drained, washed with water, dried and recrystallized.
Yield: 87%.
Melting point: 210.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.78 5.01 13.94 10.63Found 59.91 5.03 14.08 10.46______________________________________
IR spectrum (KBr): OH band at 3280-3150 cm.sup.-1, NH band CH bands at 3100, 3000 and 2970 cm.sup.-1, CO band at 1630 cm.sup.-1, main bands at 1580, 1485, 1440, 1325, 1185, 1100, 935, 720 and 710 cm.sup.-1.
EXAMPLE 25
5,6-dihydro-6-(2-hydroxyimino-1-pentyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described above, starting with the compound of Example 1.
Yield: 82%.
Melting point: 110.degree. C. (Ethyl ether).
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.39 5.65 13.85 10.57Found 59.28 5.77 13.70 10.59______________________________________
IR spectrum (KBr): OH band at 3280-3100 cm.sup.-1, NH band CH bands at 3100, 2960, 2930 and 2880 cm.sup.-1, CO band at 1640 cm.sup.-1, main bands at 1540, 1490, 1440, 1325, 1090, 955, 780 and 710 cm.sup.-1.
EXAMPLE 26
5,6-dihydro-6-(2-hydroxyiminophenethyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from 5,6-dihydro-6-(2-oxophenethyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine according to the process described above.
Yield: 82%.
Melting point: 228.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N %______________________________________Theory 64.08 4.48 12.45Found 63.93 4.60 12.32______________________________________
IR spectrum (KBr): OH band at 3300 cm.sup.-1, NH band CH bands at 3100, 3000 and 2830 cm.sup.-1, CO band at 1630 cm.sup.-1, main bands at 1495, 1450, 1325, 1150, 960, 770, 720 and 695 cm.sup.-1.
EXAMPLE 27
5,6-dihydro-6-[2-hydroxyimino-2-(4-methylphenyl)-2-ethyl]-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound of Example 4 according to the process described in Example 22.
Yield: 74%.
Melting point: 190.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 64.95 4.84 11.96 9.12Found 65.61 5.12 11.68 8.81______________________________________
IR spectrum (KBr): OH band at 3300-3140 cm.sup.-1, NH band CH bands at 3100, 2970 and 2850 cm.sup.-1, CO band at 1625 cm.sup.-1, main bands at 1535, 1490, 1445, 1325, 1185, 960, 820 and 720 cm.sup.-1.
EXAMPLE 28
6-(2-cyclopropyl-2-methoxyimino-1-ethyl)-5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 1 g of methoxylamine hydrochloride and 1.15 g of sodium acetate in 10 ml of water is added to a solution of 1 g of the compound of Example 2 in 150 ml of ethanol.
After the reaction mixture has been heated to reflux for one hour, the ethanol is removed under vacuum. After cooling, the residual solid is ground in 150 ml of water, drained, dried and recrystallized.
Yield: 82%.
Melting point: 152.degree. C. (Ethyl ether), beige crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 60.93 5.43 13.32 10.16Found 61.33 5.40 12.50 10.48______________________________________
IR spectrum (KBr): NH bands at 3270 and 3190 cm.sup.-1, CH bands at 3100, 2960 and 2930 cm.sup.-1, CO band at 1645 cm.sup.-1, main bands at 1535, 1490, 1435, 1330, 1100, 1005 and 715 cm.sup.-1.
EXAMPLE 29
5,6-dihydro-6-(2-methoxyimino-1-propyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was obtained according to the process described in Example 22, but replacing hydroxylamine hydrochloride by methoxylamine hydrochloride.
Yield: 93%.
Melting point: 138.degree. C. (Ethyl ether), white flocculent product.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.11 5.22 14.52 11.08Found 57.99 5.16 14.47 11.10______________________________________
IR spectrum (KBr): NH band at 3300 cm.sup.-1, CH bands at 3100, 2950, 2930, 2890 and 2815 cm.sup.-1, CO band at 1630 cm.sup.-1, main bands at 1530, 1480, 1425, 1320, 1180, 1145, 1040, 710 and 690 cm.sup.-1.
EXAMPLE 30
6-hydroxy-4-methoxy-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A suspension of 10 g (0.049 mole) of 3-cyano-2-(2-formyl-1-pyrrolyl)thiophene in 100 ml of 6N sodium hydroxide and 250 ml of methanol is heated slowly to 40.degree. C. for one hour until dissolution is complete. The methanol is then removed under vacuum and the liquid residue is poured into 500 ml of water. The precipitate formed is
Yield: 77%.
Melting point: 200.degree. C. (Acetone), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 56.39 4.30 11.95 13.68Found 56.39 4.39 12.00 13.83______________________________________
IR spectrum (KBr): OH band at 3380 cm.sup.-1, CH bands at 3100, 2980 and 2940 cm.sup.-1, C.dbd.N band at 1640 cm.sup.-1, main bands at 1540, 1480, 1330, 1260, 1100, 700 and 690 cm.sup.-1.
EXAMPLE 31
4-ethoxy-6-hydroxy-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was obtained as described in Example 30, but replacing methanol by ethanol.
Yield: 67%.
Melting point: 140.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.04 4.87 11.28 12.91Found 58.46 5.16 10.92 12.53______________________________________
IR spectrum (KBr): OH band at 3400 cm.sup.-1, CH bands at 3080, 2960, 2920 and 2850 cm.sup.-1, C.dbd.N band at 1620 cm.sup.-1, main bands at 1545, 1470, 1325, 1270, 1100, 785 and 700 cm.sup.-1.
EXAMPLE 32
6-hydroxy-4-propoxy-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was obtained as described in Example 30, but replacing methanol by 1-propanol.
Yield: 62%.
Melting point: 120.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.52 5.38 10.68 12.22Found 59.50 5.30 10.72 12.21______________________________________
IR spectrum (KBr): OH bands between 3400 and 3300 cm.sup.-1, CH bands at 3100 and 2960 cm.sup.-1, C.dbd.N band at 1630 cm.sup.-1, main bands at 1540, 1480, 1335, 1260, 1100, 1005 and 690 cm.sup.-1.
EXAMPLE 33
4-allyloxy-6-hydroxy-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared as described in Example 30, but using allyl alcohol as an alcohol.
Yield: 64%.
Melting point: 122.degree. C. (Ethyl ether), white flocculent product.
______________________________________Elemental analysis: C % H % S %______________________________________Theory 59.98 4.65 12.32Found 60.15 4.68 12.20______________________________________
IR spectrum (KBr): OH band at 3360 cm.sup.-1, CH bands at 3100, 3000, 2920 and 2860 cm.sup.-1, C.dbd.N band at 1630 cm.sup.-1, main bands at 1535, 1480, 1320, 1245, 1085, 980 and 675 cm.sup.-1.
EXAMPLE 34
4-methoxy-6-oxo-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 10 g of the compound of Example 30 and 20 g of potassium permanganate in 500 ml of acetone is stirred at room temperature for 4 hours. The precipitated manganese oxides are filtered off, the acetone is removed under vacuum and the residue is recrystallized.
Yield: 50%.
Melting point: 154.degree. C. (Ethyl ether), white needles.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 56.89 3.47 12.06 13.80Found 57.01 3.31 12.02 13.61______________________________________
IR spectrum (KBr): CH bands at 3110, 3075 and 2950 cm.sup.-1, C.dbd.O band at 1630 cm.sup.-1, C.dbd.N band at 1600 cm.sup.-1, main bands at 1550, 1545, 1445, 1355, 1330, 1280, 1190, 1020, 770 and 725 cm.sup.-1.
EXAMPLE 35
4-ethoxy-6-oxo-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound of Example 31 according to the process described above.
Yield: 55%.
Melting point: 188.degree. C. (Ethyl ether), white needles.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.52 4.09 11.37 13.02Found 58.48 4.04 11.29 12.93______________________________________
IR spectrum (KBr): CH bands at 3120, 3070 and 2990 cm.sup.-1, C.dbd.O band at 1635 cm.sup.-1, C.dbd.N band at 1590 cm.sup.-1, main bands at 1550, 1540, 1350, 1325, 1265, 1180, 1030, 765 and 720 cm.sup.-1.
EXAMPLE 36
6-oxo-4-propoxy-6H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound described in Example 32 according to the process described in Example 34.
Yield: 55%.
Melting point: 130.degree. C. (Ethyl ether), white needles.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.98 4.65 10.76 12.32Found 59.84 4.55 10.75 12.45______________________________________
IR spectrum (KBr): CH bands at 3310, 3075, 2985, 2940 and 2880 cm.sup.-1, C.dbd.O band at 1640 cm.sup.-1, C.dbd.N band at 1600 cm.sup.-1, main bands at 1555, 1540, 1440, 1320, 1260, 1170, 980, 730 and 705 cm.sup.-1.
EXAMPLE 37
5,6-dihydro-4-methoxy-6-oxo-4H-pyrrolo[1,2-a]thieno-[3,2-f][1,4]diazepine
1.30 g of sodium borohydride are added in small portions to a solution of 2 g of the compound of Example 34 in 200 ml of methanol, and the reaction mixture is then stirred at room temperature for 3 hours. The methanol is then removed under vacuum and the solid residue is ground in 200 ml of water, drained, washed with water, dried and recrystallized.
Yield: 74%.
Melting point: 205.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 56.40 4.30 11.96 13.68Found 56.39 4.21 11.99 13.52______________________________________
IR spectrum (KBr): NH band at 3260 cm.sup.-1, CH bands at 3100, 3000, 2950 and 2840 cm.sup.-1, CO band at 1650 cm.sup.-1, main bands at 1590, 1470, 1400, 1320, 1075, 770 and 715 cm.sup.-1.
EXAMPLE 38
5,6-dihydro-4-ethoxy-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared from the compound of Example 35 according to the process described above.
Yield: 70%.
Melting point: 170.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.05 4.87 11.28 12.91Found 57.88 4.74 11.35 12.77______________________________________
IR spectrum (KBr): NH band at 3260 cm.sup.-1, CH bands at 3120, 2695 and 2880 cm.sup.-1, CO band at 1640 cm.sup.-1, main bands at 1590, 1540, 1460, 1390, 1060, 755 and 700 cm.sup.-1.
EXAMPLE 39
5,6-dihydro-6-oxo-4-propoxy-4H-pyrrolo[1,2-a]thieno-[1,4]diazepine
This compound was prepared from the compound of Example 36 according to the process described in Example 37.
Yield: 54%.
Melting point: 160.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.52 5.38 10.68 12.22Found 59.27 5.25 10.67 12.06______________________________________
IR spectrum (KBr): NH band at 3295 cm.sup.-1, CH bands at 3105, 2930 and 2880 cm.sup.-1, CO band at 1650 cm.sup.-1, main bands at 1595, 1545, 1460, 1390, 1305, 1075, 1030 and 760 cm.sup.-1.
EXAMPLE 40
5,6-dihydro-6-hydroxy-4-oxo-4H-pyrrolo[1,2-a]thieno-[3,2-f]1,4]diazepine
0.5 ml of triethylamine is added to a suspension of 2 g of 3-carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene in 60 ml of water, and the reaction mixture is then stirred for 12 hours at room temperature. The product passes gradually into solution and then reprecipitates. The precipitate obtained is drained, washed with water, dried and recrystallized.
Yield: 68%.
Melting point: 166.degree. C. (Water), beige needles.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 54.53 3.66 12.71 14.56Found 54.36 3.69 12.58 14.39______________________________________
IR spectrum (KBr): OH band at 3340 cm.sup.-1, NH band at 3220 cm.sup.-1, CH bands at 3120 and 3110 cm.sup.-1, C.dbd.O band at 1610 cm.sup.-1, main bands at 1500, 1450, 1320, 1205, 1005, 830 and 720 cm.sup.-1.
Proton NMR spectrum (solvent DMSO-d.sub.6):
______________________________________ ##STR32##H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6ppm ppm ppm ppm ppm ppm ppm Other protons______________________________________7.20 7.20 6.25 6.25 7.18 8.94 5.62 OH: 6.33______________________________________
EXAMPLE 41
5.6-dihydro-6-methylamino-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f]1,4]diazepine
Process A
10 ml of a 35% aqueous solution of methylamine are added to a suspension of 1.5 g of the compound obtained in Example 40 in 50 ml of water. The precipitate formed at the end of the reaction is drained, washed with water, dried and recrystallized.
Yield: 88%.
Process B
8 ml of a 35% aqueous solution of methylamine in 50 ml of water are added to a solution of 1 g of 3-carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene in 50 ml of acetonitrile. The reaction mixture is heated to reflux for approximately 3 hours. The acetonitrile is then removed under vacuum and the residue recrystallized in ethyl ether.
Yield: 84%.
Melting point: 168.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 56.63 4.75 18.01 13.74Found 56.55 4.73 17.88 13.69______________________________________
IR spectrum (KBr): NH bands at 3320, 3250 and 3170 cm.sup.-1, CH bands at 3100, 3040, 2980, 2930 and 2860 cm.sup.-1, C.dbd.O band at 1635 cm.sup.-1, main bands at 1535, 1490, 1435, 1330, 1315, 1120, 1090, 820, 750 and 705 cm.sup.-1.
EXAMPLE 42
5,6-dihydro-6-ethylamino-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as described in Example 41 (Process A or B), using an aqueous solution of ethylamine (70%) instead of an aqueous solution of methylamine.
Yield: (Process A)=89%, (Process B)=80%.
Melting point: 156.degree. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.28 5.30 16.99 12.96Found 58.26 5.25 16.99 12.87______________________________________
IR spectrum (KBr): NH bands at 3300, 3240 and 3140 cm.sup.-1, CH bands at 3020, 2940 and 2850 cm.sup.-1, C.dbd.O bands at 1630 cm.sup.-1, main bands at 1525, 1475, 1450, 1420, 1315, 1115, 1070, 770, 705, 690 and 660 cm.sup.-1.
EXAMPLE 43
5,6-dihydro-4-oxo-6-propylamino-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepin
To obtain this compound, the procedure is as in Example 41 (Process A), using propylamine instead of methylamine.
Melting point: 130.degree. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.75 5.78 16.08 12.87Found 59.63 5.82 15.95 12.13______________________________________
IR spectrum (KBr): NH bands at 3340, 3260 and 3170 cm.sup.-1, CH bands at 3100, 3040, 2960, 2930 and 2860 cm.sup.-1, C.dbd.O band at 1635 cm.sup.-1, main bands at 1540, 1495, 1435, 1330, 1095 and 740 cm.sup.-1.
EXAMPLE 44
5,6-dihydro-6-isopropylamino-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in the previous example, using isopropylamine instead of propylamine.
Yield: 76%.
Melting point: 152.degree. C. (Ethyl ether), yellow crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.75 5.78 16.08 12.27Found 59.67 5.88 16.18 12.12______________________________________
IR spectrum (KBr): NH bands at 3330, 3250 and 3170 cm.sup.-1, CH bands at 3040, 2970 and 2870 cm.sup.-1, CO band at 1625 cm.sup.-1, main bands at 1485, 1430, 1320, 1180, 1090, 815, 790, 750, 690 and 670 cm.sup.-1.
EXAMPLE 45
5,6-dihydro-6-dimethylamino-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in Example 43, using a 40% aqueous solution of dimethylamine.
Yield: 63%.
Melting point: 180.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.28 5.30 16.99 12.96Found 58.12 5.22 16.91 13.09______________________________________
IR spectrum (KBr): NH bands at 3280 and 3180 cm.sup.-1, CH bands at 3060, 2950, 2940, 2820 and 2780 cm.sup.-1, CO band at 1650 cm.sup.-1, main bands at 1540, 1490, 1440, 1325, 1300, 1215, 995, 810, 800, 715 and 695 cm.sup.-1.
EXAMPLE 46
5,6-dihydro-6-diethylamino-4-oxo- 4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in Example 45, starting with 1 g of the compound of Example 40 and 3 ml of diethylamine in 50 ml of water.
Yield: 65%.
Melting point: 137.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 61.06 6.22 15.26 11.64Found 60.99 6.21 15.31 11.86______________________________________
IR spectrum (KBr): NH bands at 3275 and 3185 cm.sup.-1, CH bands at 3050, 2960, 2930 and 2820 cm.sup.-1, CO band at 1645 cm.sup.-1, main bands at 1545, 1500, 1445, 1325, 1215, 1090, 1025 and 705 cm.sup.-1.
EXAMPLE 47
5,6-dihydro-4-oxo-6-(1-pyrrolidinyl)-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in Example 45, using 8 g of the compound of Example 40 and 4 ml of pyrrolidine in ml of water.
Yield: 89%.
Melting point: 176.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 61.52 5.53 15.37 11.73Found 61.65 5.58 15.46 11.80______________________________________
IR spectrum (KBr): NH bands at 3280 and 3180 cm.sup.-1, CH bands at 3060, 2950, 2930 and 2790 cm.sup.-1, CO band at 1645 cm.sup.-1, main bands at 1540, 1500, 1445, 1325, 1310, 1215, 1125, 895, 825, 805, 720 and 705 cm.sup.-1.
EXAMPLE 48
5,6-dihydro-4-oxo-6-piperidino-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as in Example 45, using 1.5 g of the compound of Example 40 and 3 ml of piperidine in ml of water.
Yield: 86%.
Melting point: 150.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 62.69 5.96 14.62 11.16Found 61.43 5.87 14.50 10.90______________________________________
IR spectrum (KBr): NH bands at 3270 and 3190 cm.sup.-1, CH bands at 3060, 2940, 2860, 2800 and 2760 cm.sup.-1, CO band at 1650 cm.sup.-1, main bands at 1540, 1495, 1445, 1325, 1310, 1230, 1100, 980, 880 and 710 cm.sup.-1.
EXAMPLE 49
5,6-dihydro-4-oxo-6-(4-morpholinyl)-4H-pyrrolo[1,2-a]thieno[3,2-][1,4]diazepine
The procedure is as in Example 48, using 3 ml of morpholine.
Yield: 65%.
Melting point: 180.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.11 5.22 14.52 11.08Found 58.13 5.23 14.64 10.94______________________________________
IR spectrum (KBr): NH bands at 3280 and 3200 cm.sup.-1, CH bands at 3110, 3060, 2960, 2910 and 2810 cm.sup.-1, CO band at 1645 cm.sup.-1, main bands at 1545, 1495, 1440, 1330, 1110, 1000, 870, 790 and 700 cm.sup.-1.
EXAMPLE 50
5,6-dihydro-4-oxo-6-(4-ethoxycarbonylpiperazino)-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as for Example 45, starting with 2 g of the compound of Example 40 and 5 ml of N-ethoxycarbonylpiperazine in 100 ml of water.
Yield: 80%.
Melting point: 200.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 56.65 5.59 15.54 8.89Found 56.23 5.41 15.34 8.45______________________________________
IR spectrum (KBr): NH bands at 3280 and 3180 cm.sup.-1, CH bands at 3050, 2940, 2810 and 2775 cm.sup.-1, CO bands at 1690 and 1650 cm.sup.-1, main bands at 1435, 1250, 1230, 1120, 996 and 715 cm.sup.-1.
EXAMPLE 51
5,6-dihydro-4-oxo-6-anilino-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 1 9 of the compound of Example 1 in 20 ml of dioxane is added in a sin91e portion to an emulsion of 5 ml of aniline in 10 cm.sup.3 of 1N sodium hydroxide, and the reaction medium is then stirred at room temperature for 6 hours.
300 ml of water are then poured into the emulsion obtained. The precipitate formed is drained, washed with water, dried and recrystallized.
Yield: 75%.
Melting point: 160.degree. C. (Ethyl ether), white flocculent product.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 65.07 4.44 14.23 10.85Found 64.67 4.56 14.01 11.10______________________________________
IR spectrum (KBr): NH bands at 3340, 3280 and 3190 cm.sup.-1, CH bands at 3110 and 3060 cm.sup.-1, CO band at 1645 cm.sup.-1, main bands at 1605, 1500, 1330, 1310, 1260, 755 and 700 cm.sup.-1.
EXAMPLE 52
5,6-dihydro-4-oxo-6-(N-benzylamino)-4H-pyrrolo-[1,2-a]thieno[3,2-f][1,4]diazepine
The procedure is as for Example 45, starting with 2 g of the compound of Example 40 and 7.5 ml of benzylamine in 100 ml of water.
Yield: 70%.
Melting point: 148.degree. C. (Ethyl ether), white flocculent product.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 66.00 4.89 13.58 10.36Found 65.84 4.91 13.44 10.55______________________________________
IR spectrum (KBr): NH bands at 3340 and 3220 cm.sup.-1, CH bands at 3090 and 2900 cm.sup.-1, CO band at 1635 cm.sup.-1, main bands at 1500, 1465, 1330, 1230, 740, 715 and 700 cm.sup.-1.
EXAMPLE 53
5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
0.62 g of sodium borohydride is added in small portions to a solution of 1.5 g of the compound obtained in Example 49 in 150 ml of methanol, and the reaction mixture is stirred at room temperature for 15 minutes and then heated to reflux for 30 min. The methanol is then removed under vacuum and the solid residue ground in 200 ml of water. The precipitate formed is drained, washed with water, dried and recrystallized.
Yield: 86%.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 58.81 3.95 13.72 15.70Found 59.02 3.87 13.78 15.65______________________________________
IR spectrum (KBr): NH bands at 3280 and 3170 cm.sup.-1, CH bands at 3090 and 2900 cm.sup.-1, CO band at 1660 cm.sup.-1, main bands at 1540, 1495, 1460, 1225, 1095, 910, 750, 680 and 620 cm.sup.-1.
Proton nuclear magnetic resonance spectrum (solvent CDCl.sub.3):
______________________________________ ##STR33##H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NHppm ppm ppm ppm ppm ppm CH.sub.2 6______________________________________7.39 6.93 6.13 6.29 6.96 6.60 4.29______________________________________
EXAMPLE 54
ethyl (5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepin-6-yl)cyanoacetate
2 ml of ethyl cyanoacetate and 0.5 ml of triethylamine are added to a solution of 1 g of the compound of Example 40 in 100 ml of acetonitrile. The reaction mixture is stirred at room temperature for 12 hours. The acetonitrile is then removed under vacuum. The oily residue is ground in 200 ml of water. The precipitate formed is drained, washed with water, dried and recrystallized.
Yield: 49%.
Melting point: 208.degree. C. (Ethyl ether), yellow crystal.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 57.13 4.16 13.33 10.17Found 56.99 4.17 13.15 10.03______________________________________
IR spectrum (KBr): NH bands at 3400 and 3220 cm.sup.-1, CH bands at 3050 and 3300 cm.sup.-1, C.dbd.N band at 2270 cm.sup.-1, CO bands at 1720 (ester) and 1685 (lactam) cm.sup.-1, main bands at 1590, 1470, 1425, 1240, 1095, 760 and 710 cm.sup.-1.
Proton nuclear magnetic resonance spectrum (solvent DMSO-d.sub.6):
______________________________________ ##STR34##H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6ppm ppm ppm ppm ppm ppm ppm Other protons______________________________________7.76 7.50 7.43 7.61 7.67 7.61 6.64 CH.sub.2 : 4.20 CH.sub.3 : 1.22______________________________________
EXAMPLE 55
5,6-dihydro-6-(1-methylethoxy)-4-oxo-4H-pyrrolo-[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 1 g of 3-carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene in 60 ml of isopropanol is heated to reflux for one hour. The isopropanol is then removed under vacuum and the residual solid recrystallized.
Yield: 93%.
Melting point: 165.degree. C. (Isopropanol), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 59.52 5.37 10.67 12.22Found 59.71 5.36 10.84 12.18______________________________________
IR spectrum (KBr): NH bands at 3270 and 3180 cm.sup.-1, CH bands at 3050, 2960 and 2880 cm.sup.-1, C.dbd.O band at 1645 cm.sup.-1, main bands at 1540, 1500, 1320, 1230, 1215, 1020, 730 and 705 cm.sup.-1.
Process B
Same protocol as ior Process A, but 1 g of the compound described in Example 40 is used instead of 3-carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene.
Yield: 93%.
EXAMPLE 56
6-benzyloxy-5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
0.0050 mole of benzyl alcohol is added to a solution of 0.0045 mole of the compound of Example 40 in 100 ml of acetonitrile, and the reaction mixture is then heated to reflux for 90 min. The acetonitrile is then removed under vacuum. The residual oil which crystallizes on addition of petroleum ether is recrystallized.
Yield: 86%.
Melting point: 150.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 65.79 4.55 10.33 9.02Found 65.41 4.37 10.45 9.22______________________________________
IR spectrum (KBr): NH bands at 3260 and 3190 cm.sup.-1, CH bands at 3100, 3060, 2920 and 2860 cm.sup.-1, C.dbd.O band at 1635 cm.sup.-1, main bands at 1540, 1490, 1440, 1310, 1025, 920, 710 and 685 cm.sup.-1.
EXAMPLE 57
5,6-dihydro-6-(N',N'-dimethylhydrazino)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
2 ml of dimethyl hydrazine are added to a suspension of 1 g of 3-carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene in 300 ml of water. The reaction mixture is heated to 50.degree. C. for 30 min and then stirred at room temperature for 12 hours. The orange solution obtained is extracted with twice 100 ml of ethyl ether. The organic phases are combined, washed with water, dried over sodium sulfate and taken down under vacuum. The residual oil which crystallizes on addition of petroleum ether is recrystallized.
Yield: 76%.
Melting point: 116.degree. C. (Ethanol) pink crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 54.94 5.38 21.36 12.22Found 54.11 5.10 20.61 12.05______________________________________
IR spectrum (KBr): NH bands at 3280 and 3140 cm.sup.-1, CH bands at 3000, 2950, 2860 and 2790 cm.sup.-1, CO band at 1675 cm.sup.-1, main bands at 1450, 1420, 1330, 1050, 860, 795 and 715 cm.sup.-1.
EXAMPLE 58
5,6-dihydro-1-oxo-6-(N'-phenylhydrazino)-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 57, but using phenylhydrazine.
Yield: 71%.
Melting point: 158.degree. C. (Ethyl ether), gray crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 61.92 4.55 18.05 10.33Found 62.12 4.66 17.89 10.43______________________________________
IR spectrum (KBr): NH bands at 3460, 3320, 3280 and 3150 cm.sup.-1, CH bands at 3100 and 3040 cm.sup.-1, CO band at 1660 cm.sup.-1, main bands at 1580, 1490, 1460, 1280, 1255, 1125, 750, 715 and 690 cm.sup.-1.
EXAMPLE 59
5,6-dihydro-6-[N'-(2,5-dinitrophenylhydrazino)]-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described in Example 57, but using 2,5-dinitrophenylhydrazine.
Yield: 65%.
Melting point: 238.degree. C. (Ethyl ether/acetone), red crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 48.10 3.01 8.00 21.00Found 47.92 2.98 8.02 20.79______________________________________
IR spectrum (KBr): NH bands at 3270 and 3120 cm.sup.-1, CH bands at 3110 and 3090 cm.sup.-1, C.dbd.O band at 1650 cm.sup.-1, main bands at 1615, 1500, 1420, 1340, 1320, 1135, 1090 and 715 cm.sup.-1.
EXAMPLE 60
5,6-dihydro-6-(methoxycarbonylmethylthio)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
A solution of 1 g of 3-carbamoyl-2-(2-formyl-1-pyrrolyl)thiophene in 100 ml of acetonitrile is treated with 0.43 ml (0.005 mole) of methyl thioglycolate, and the reaction mixture is stirred overnight at room temperature. The acetonitrile is then removed under vacuum. The residual oil which crystallizes on addition of petroleum ether is recrystallized.
Yield 72%.
Melting point: 158.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 50.64 3.92 9.08 20.73Found 50.50 3.83 9.02 20.95______________________________________
IR spectrum (KBr): NH bands at 3360 and 3280 cm.sup.-1, CH bands at 3050 and 2950 cm.sup.-1, CO bands at 1730 (ester) and 1645 (lactam) cm.sup.-1, main bands at 1535, 1495, 1440, 1310, 1240, 1165, 790, 735 and 705 cm.sup.-1.
EXAMPLE 61
5,6-dihydro-6-phenylthio-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
This compound was prepared according to the process described Example 60, but using thiophenol instead of methyl thioglycolate.
Yield: 78%.
Melting point: 142.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 61.51 3.87 8.97 20.52Found 60.65 3.82 9.13 20.69______________________________________
IR spectrum (KBr): NH bands at 3370 and 3160 cm.sup.-1, CH bands at 3120, 3010 and 2930 cm.sup.-1, CO band at 1645 cm.sup.-1, main bands at 1550, 1500, 1480, 1325, 1220, 1065, 775, 720 and 690 cm.sup.-1.
EXAMPLE 62
3-(5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepin-6-ylthio)propionic acid
This compound was prepared according to the process described in Example 60, but using 3-mercaptopropionic acid instead of methyl thioglycolate.
Yield: 50%.
Melting point: 153.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 50.64 3.92 9.08 20.79Found 50.48 3.89 9.07 20.71______________________________________
IR spectrum (KBr): OH band at 3450 cm.sup.-1, NH bands at 3260 and 3200 cm.sup.-1, CH bands at 3060 and 2940 cm.sup.-1, C.dbd.O bands at 1700 (acid) and 1645 (lactam) cm.sup.-1, main bands at 1550, 1500, 1430, 1245, 1190, 925 and 710 cm.sup.-1.
EXAMPLE 63
3-(5,6-dihydro-4-oxo-4H-pyrrolo[1,2-a]thieno[1,4]diazeoin-6-ylthio)propionamide
0.50 ml (0.0035 mole) of triethylamine is added in a single portion to a suspension, cooled beforehand to 0.degree. C., of 1 g of the compound of Example 62 in 150 ml of ethyl ether, the reaction mixture is then stirred for 20 min at the same temperature, 0.30 ml of ethyl chloroformate are thereafter added dropwise and stirring is continued for 20 min at 0.degree. C. The product gradually passes into solution and the appearance of a flocculent precipitate of triethylamine hydrochloride is observed. The latter is filtered off and a stream of gaseous ammonia is passed into the ethereal solution, still maintained at 0.degree. C., for 20 seconds. The white precipitate obtained is drained, washed with ethyl ether and recrystallized.
Yield: 25%.
______________________________________Elemental analysis: C % H % N % S %______________________________________Theory 50.80 4.26 13.67 20.86Found 50225 4.67 14.03 21.66______________________________________
IR spectrum (KBr): NH bands at 3460, 3340 and 3170 cm.sup.-1, CH bands at 3040, 2960 and 2920 cm.sup.-1, C.dbd.O band at 1660 cm.sup.-1, main bands at 1490, 1430, 1410, 1425, 1090, 750 and 710 cm.sup.-1.
EXAMPLE 64
5,6-dihydro-6-(2-benzoyloxypropyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
0.55 ml of benzoyl chloride is added to a solution of 0.7 g of 5,6-dihydro-6-(2-hydroxypropyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine (compound of Example 12) in 10 ml of pyridine. The reaction medium is stirred for 24 hours at room temperature and the pyridine is then removed under vacuum.
The residue is taken up with 100 ml of water and the product extracted with 150 ml of ethyl ether.
The organic phase is washed with water, dried over magnesium sulfate and then concentrated.
The residual oil which crystallizes on addition of petroleum ether is recrystallized in ethyl ether.
Yield: 25%.
Melting point: 95.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis C % H % N % S %______________________________________Theory 65.56 4.95 7.64 8.75Found 66.01 4.72 6.22 8.92______________________________________
IR spectrum (KBr): NH bands at 3260 and 3140 cm.sup.-1, CH bands at 2980, 2960 and 2890 cm.sup.-1, CO bands at 1730 and 1675 cm.sup.-1, main bands at 1550, 1500, 1290, 1245, 1100 and 720 cm.sup.-1.
EXAMPLE 65
5,6-dihydro-6-(2-hydrazono-1-pentyl)-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine
0.6 ml of hydrazine hydrate is added to a solution of 1 g of 5,6-dihydro-4-oxo-6-(2-oxo-1-pentyl)-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine (Example 1) in 250 ml of ethanol, and the reaction mixture is then heated to reflux for one hour. The ethanol is then removed under vacuum and the residual solid ground in 100 ml of water.
The precipitate formed is drained, washed, dried and recrystallized.
Yield: 76%.
Melting point: 158.degree. C. (Ethyl ether), white crystals.
______________________________________Elemental analysis: C % H % S % N %______________________________________Theory 59.78 5.69 18.59 10.64Found 59.45 5.88 18.21 10.31______________________________________
IR spectrum (KBr): NH bands at 3410, 3370 and 3190 cm.sup.-1, CH bands at 3050, 2970 and 2880 cm.sup.-1, CO band at 1640 cm.sup.-1, main bands at 1490, 1435, 1335, 1090 and 715 cm.sup.-1.
TABLE I__________________________________________________________________________Compounds of formula I.sub.A ##STR35##Com- H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6 Other protonspound Solvent R.sub.5 (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example CDCl.sub.3 CH.sub.2 CH.sub.2 CH.sub.3 7.36 6.96 6.00 6.28 6.96 7.06 4.96 CH.sub.2 : 3.071 CH.sub.2 : 2.46 CH.sub.3 : 0.90 CH.sub.2 : 1.60Example 2 CDCl.sub.3 ##STR36## 7.38 6.96 6.05 6.29 6.96 6.50 4.96 CH.sub.2 : 3.22 CH: 1.96 2CH.sub.2 : 1.10 and 0.98Example 3 CDCl.sub.3 ##STR37## 7.34 6.92 5.98 6.26 6.92 6.73 4.95 CH.sub.2 : 3.03 CH.sub.2 : 2.86 CH.sub.2 : 2.82Example 4 CDCl.sub.3 ##STR38## 7.32 6.92 6.05 6.27 6.97 6.70 5.11 CH.sub.2 : 3.58 CH.sub.3 : 2.42 C.sub.6 H.sub.4 : 7.84 and 7.26Example 5 CDCl.sub.3 ##STR39## 7.31 6.95 6.04 6.27 6.95 7.02 5.10 CH.sub.2 : 3.58 CH.sub.3 : 2.48 C.sub.6 H.sub.4 : 7.68, 7.40, 7.28Example 6 CDCl.sub.3 ##STR40## 7.37 6.95 6.05 6.30 6.96 6.50 5.13 CH.sub.2 : 3.66 C.sub.6 H.sub.3 : 7.29, 7.06 and 6.95 2CH.sub. 3 : 3.80 and 3.78Example 7 DMSO- d.sub.6 ##STR41## 7.35 7.00 6.23 6.23 7.35 8.20 4.80 CH.sub.2 : 3.67 C.sub.6 H.sub.4 Cl: 8.10, 8.0, 7.60Example 8 CDCl.sub.3 ##STR42## 7.37 6.97 6.10 6.29 6.97 6.59 5.13 CH.sub.2 : 3.55 C.sub.4 H.sub.3 S: 7.73 and 7.16Example 9 CDCl.sub.3 ##STR43## 7.35 6.93 6.07 6.29 6.93 6.45 5.19 CH.sub.2 : 3.55 C.sub.6 H.sub.4 F: 7.91, 6.93 and 7.15Example 10 CDCl.sub.3 ##STR44## 7.38 6.98 6.09 6.29 6.98 6.47 5.11 CH.sub.2 : 3.48 C.sub.4 H.sub.3 O:7.26, 7.38 and 6.51Example 11 CDCl.sub.3 ##STR45## 7.38 6.93 6.03 6.28 6.96 6.13 4.98 CH.sub.2 : 3.11 3CH.sub.3 :__________________________________________________________________________ 1.54
TABLE II__________________________________________________________________________Compounds of formula I.sub.B ##STR46## H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6 Other protonsCompound Solvent (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________ R.sub.5Example 13 CDCl.sub.3 CH.sub.2 CH.sub.2 CH.sub.3 7.34 6.92 6.04 6.27 6.92 7.03 4.66 CH: 4.04 OH: 2.40 CH.sub.3 : 0.94 CH.sub.2 : 2.10 CH.sub.2 : 1.50 and 1.34Example 14 CDCl.sub.3 ##STR47## 7.37 6.94 6.06 6.31 6.94 7.07 4.70 CH: 3.29 OH: 2.40 CH.sub.2 : 2.28 CH: 1.04 CH.sub.2 : 0.58 and 0.31 RExample 15 CDCl.sub.3 ##STR48## 7.34 6.93 6.09 6.30 6.33 7.34 5.17 CH.sub.2 : 2.37 CH: 4.62 C.sub.6 H.sub.5 : 7.34Example 17 DMSO-d.sub.6 ##STR49## 7.20 7.08 6.09 6.26 7.08 8.13 4.46 OH: 4.52 CH: 3.64 CH.sub.2 : 2.88 2CH.sub.3 : 0.88 CH: 1.90Example 18 CDCl.sub.3 ##STR50## 7.38 6.94 6.14 6.31 6.94 6.74 5.36 OH: 4.70 OH: 2.56 2CH.sub.3 : 3.81 CH.sub.2 : 2.38 C.sub.6 H.sub.3 : 7.07 and__________________________________________________________________________ 6.81
TABLE III__________________________________________________________________________Compounds of formula I.sub.c ##STR51## H.sub.2 H.sub.3 H.sub.10 H.sub.11 H.sub.12 H.sub.8 H.sub.9a Other protonsCompound Solvent R.sub.5 (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) CH.sub.2 '.sub.9 (ppm)__________________________________________________________________________Example 19 CFCl.sub.3 CH.sub.3 7.36 7.36 6.40 6.52 7.36 5.00 5.15 CH: 2.54 CH.sub.3 : 1.42 CH: 2.31Example 20 CDCl.sub.3 CH.sub.2 CH.sub.2 CH.sub.3 7.37 7.37 6.41 6.48 7.35 4.84 5.15 CH: 2.71 CH.sub.2 :1.68 and 1.45 CH: 2.35 CH.sub.3 : 0.94Example 21 CDCl.sub.3 ##STR52## 7.26 7.26 6.22 6.32 7.26 5.30 4.50 2.52 CH.sub.3 : 2.32 C.sub.6 H.sub.4 : 8.59, 7.40,__________________________________________________________________________ 7.26
TABLE IV__________________________________________________________________________Compounds of formula I.sub.D ##STR53## H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 Other protonsCompound Solvent R.sub.6 R.sub.5 (ppm) (ppm) (ppm) (ppm) (ppm) NH H.sub.6 (ppm)__________________________________________________________________________Example 22 CDCl.sub.3 H CH.sub.3 7.40 7 6.37 6.13 7.0 7.89 4.57 OH: 10.72 CH.sub.2 : 2.89 CH.sub.3 : 1.93Example 23 CDCl.sub.3 H ##STR54## 7.40 6.94 6.34 6.17 6.98 7.67 4.57 OH: 9.63 CH.sub.2 : 2.55 CH: 3.66 2CH.sub.3 : 1.10Example 24 CDCl.sub.3 H ##STR55## 7.40 6.95 6.34 6.16 7.00 7.40 4.88 OH: 4.88 CH: 1.49 2CH.sub.2 : 0.75Example 25 CDCl.sub.3 H CH.sub.2 CH.sub.2 CH.sub.3 7.40 6.95 6.34 6.14 6.97 7.50 4.6 CH.sub.2 : 2.9 CH.sub.2 : 1.50 and 1.19 CH.sub.3 : 0.90Example 26 CDCl.sub.3 H ##STR56## 7.33 6.95 6.29 6.07 6.95 6.63 5.15 CH.sub.2 : 3.62 C.sub.6 H.sub.5 : 7.95, 7.59, 7.47Example 27 CDCl.sub.3 H ##STR57## 7.33 6.90 6.38 6.28 7.00 10.76 4.55 CH.sub.2 : 4.12 and 3.13 CH.sub.3 : 2.33 C.sub.6 H.sub.4 : 7.55 and 7.13Example 28 CDCl.sub.3 CH.sub.3 ##STR58## 7.30 6.88 6.26 6.02 6.92 6.64 4.90 CH: 1.24 2CH.sub.2 : 0.92 and 0.88 CH.sub.3 : 3.84Example 29 DMSO-d.sub.6 CH.sub.3 CH.sub.3 7.28 7.21 6.28 6.22 7.13 8.14 4.61 CH.sub.3 : 3.71 CH.sub.3 :__________________________________________________________________________ 1.75
TABLE V__________________________________________________________________________Compounds of formula I.sub.E ##STR59## H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 H.sub.6 OH Other protonsCompound Solvent R.sub.3 (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 30 DMSO-d.sub.6 CH.sub.3 7.30 7.14 6.34 6.34 7.10 5.60 6.18 CH.sub.2 : 3.70Example 31 DMSO-d.sub.6 CH.sub.2 CH.sub.3 7.31 7.14 6.2 6.2 7.11 5.60 6.20 CH.sub.2 : 4.16 CH.sub.3 : 1.10Example 32 DMSO-d.sub.6 CH.sub.2 CH.sub.2 CH.sub.3 7.31 7.15 6.30 6.30 7.10 5.66 6.30 CH.sub.2 : 4.08 CH.sub.2 : 1.63 CH.sub.3 : 0.95Example 33 DMSO-d.sub.6 CH.sub.2 CHCH.sub.2 7.33 7.16 6.20 6.20 7.10 5.63 6.20 CH: 5.93 CH.sub.2 : 4.63 CH.sub.2 :__________________________________________________________________________ 5.21
TABLE VI__________________________________________________________________________Compounds of formula I.sub.F ##STR60## R.sub.3 H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 Other protonsCompound Solvent (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 34 DMSO-d.sub.6 CH.sub.3 7.43 7.30 7.08 6.63 7.70 CH.sub.3 : 3.93Example 35 DMSO-d.sub.6 CH.sub.2 CH.sub.3 7.40 7.30 7.01 6.60 7.65 CH.sub.2 : 4.35 CH.sub.3 : 1.34Example 36 DMSO-d.sub.6 CH.sub.2 CH.sub.2 CH.sub.3 7.45 7.31 7.08 6.65 7.68 CH.sub.2 : 4.31 CH.sub.2 : 1.78 CH.sub. 3 : 1.00__________________________________________________________________________
TABLE VII__________________________________________________________________________Compounds of formula I.sub.G ##STR61## R.sub.3 H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.4 Other protonsCompound Solvent (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 37 CDCl.sub.3 CH.sub.3 7.26 6.93 6.93 6.40 7.17 7.31 5.25 CH.sub.3 : 3.35Example 38 DMSO-d.sub.6 CH.sub.2 CH.sub.3 7.25 7.11 6.97 6.37 7.37 9.05 5.32 CH.sub.2 : 3.48 CH.sub.3 : 0.98Example 39 DMSO-d.sub.6 CH.sub.2 CH.sub.2 CH.sub.3 7.25 7.11 6.95 6.35 7.36 9.03 5.32 CH.sub.2 : 3.39 CH.sub.2 : 1.36 CH.sub.2 :__________________________________________________________________________ 0.65
TABLE VIII__________________________________________________________________________Compounds of formula I.sub.H ##STR62##Solvent used: CDCl.sub.3 R.sub.7 R.sub.8 H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6 Other protonsCompound (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 41 H CH.sub.3 7.39 6.91 6.19 6.31 6.98 6.81 5.03 CH.sub.3 : 1.75Example 42 H CH.sub.2 CH.sub.3 7.34 6.92 6.19 6.31 6.98 6.76 5.15 CH.sub.2 : 2.82 CH.sub.3 : 1.11Example 43 H CH.sub.2 CH.sub.2 CH.sub.3 7.39 6.91 6.18 6.31 6.98 6.52 5.13 CH.sub.2 : 2.73 CH.sub.2 : 1.49 CH.sub.3 : 0.88 NH: 4.82Example 44 H ##STR63## 7.38 6.92 6.17 6.31 6.97 6.83 5.20 CH: 3.08 2CH.sub.3 : 1.07Example 45 CH.sub.3 CH.sub.3 7.38 6.85 6.21 6.29 6.99 6.28 4.46 2CH.sub.3 : 2.20Example 46 CH.sub.2 CH.sub.3 CH.sub.2 CH.sub.3 7.37 6.89 6.19 6.31 6.98 6.31 5.11 2CH.sub.2 : 2.66 2CH.sub.2 : 0.98Example 47 ##STR64## 7.37 6.83 6.19 6.27 6.97 6.71 4.64 CH.sub.2 : 2.64 CH.sub.2 : 2.35 2CH.sub.2 : 1.65Example 48 ##STR65## 7.35 6.85 6.18 6.28 6.97 6.62 4.65 CH.sub.2 : 2.63 CH.sub.2 : 2.19 3CH.sub.2 : 1.39Example 49 ##STR66## 7.35 6.86 6.22 6.29 7.00 6.93 4.53 2CH.sub.2 : 3.47 CH.sub.2 : 2.50 CH.sub.2 : 2.14Example 50 ##STR67## 7.35 6.85 6.18 6.28 6.97 6.67 4.65 2X CH.sub.2 : 3.24 2X CH.sub.2 : 2.48 CH.sub.2 : 4.07 CH.sub.3 : 1.21Example 51 H ##STR68## 7.18 7.18 6.38 6.32 7.16 8.85 5.71 ##STR69##Example 52 H ##STR70## 7.29 6.90 6.18 6.30 6.97 6.48 5.12 CH.sub.2 : 3.93 ar CH: 7.29 CH.sub.2 :__________________________________________________________________________ 1.6
TABLE IX__________________________________________________________________________Compounds of formula I.sub.J ##STR71## R.sub.5 H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 HH H.sub.6 Other protonsCompound Solvent (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 55 CDCl.sub.3 ##STR72## 7.38 6.86 6.25 6.29 7.06 7.06 5.52 CH: 3.79 2CH.sub.3 : 1.71Example 56 CDCl.sub.3 ##STR73## 7.41 6.88 6.23 6.29 7.03 7.53 5.44 CH.sub.2 : 4.53 C.sub.6 H.sub. 5 : 7.20, 7.31,__________________________________________________________________________ 7.20
TABLE X__________________________________________________________________________Compounds of formula I.sub.K ##STR74## R.sub.5 R.sub.6 H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6 Other protonsCompound Solvent (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 57 CDCl.sub.3 CH.sub.3 CH.sub.3 7.48 7.21 6.53 6.79 6.94 5.52 5.15 2CH.sub.3 : 2.74 NH: 5.33Example 58 CDCl.sub.3 H ##STR75## 7.56 7.18 6.64 6.71 6.80 6.43 5.33 NH: 5.33 NH: 6.38 C.sub.6 H.sub.5 : 7.18, 7.12, 7.18, 6.70Example 59 DMSO H ##STR76## 8.00 7.08 6.28 6.28 7.19 9.94 5.54 NH: 5.85 NH: 8.79 C.sub.6 H.sub.3 : 7.30 and__________________________________________________________________________ 8.79
TABLE XI__________________________________________________________________________Compounds of formula I.sub.L ##STR77## R.sub.5 H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6 Other protonsCompound Solvent (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 60 CDCl.sub.3 CH.sub.2 CO.sub.2 CH.sub.3 7.40 6.92 6.28 6.28 7.04 6.92 5.94 CH.sub.2 : CH: 3.29 CH: 3.06 CH.sub.3 : 3.76Example 61 CDCl.sub.3 C.sub.6 H.sub.5 7.43 6.95 6.11 6.26 7.02 6.60 5.73 C.sub.6 H.sub.5 : 7.34Example 62 DMSO CH.sub.2 CH.sub.2 CO.sub.2 H 7.21 7.21 6.31 6.31 7.21 9.11 5.73 CH.sub.2 : 2.43 and 2.60 OH: 12.21Example 63 DMSO CH.sub.2 CH.sub.2 CONH.sub.2 7.25 7.25 6.27 6.27 7.25 9.00 5.68 NH.sub.2 : 7.27 and 6.73 CH.sub.2 : 2.56 and__________________________________________________________________________ 2.24
TABLE XII__________________________________________________________________________ ##STR78## H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 NH H.sub.6 H.sub.10 H.sub.11 H.sub.12 Other protonsCompound (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 64 7.35 6.93 6.10 6.16 6.93 7.40 5.15 2.27 4.44 1.32 ar CH: 7.24 7.40 7.90__________________________________________________________________________
TABLE XIII__________________________________________________________________________ ##STR79## H.sub.2 H.sub.3 H.sub.7 H.sub.8 H.sub.9 H.sub.5 H.sub.6 H.sub.6 H.sub.11 H.sub.12 H.sub.12 H.sub.12Compound (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm) (ppm)__________________________________________________________________________Example 65 7.27 7.21 6.11 6.27 7.11 7.91 4.64 2.74 2.10 1.44 0.88 5.76__________________________________________________________________________
EXAMPLE 66
Affinity for cholecystokinin receptors
The affinity of the compounds of formula (I) for cholecystokinin receptors was studied by determining the median inhibitory concentration (IC.sub.50) for the binding of iodine-125-labeled cholecystokinin to the receptors of rat pancreas plasma membranes (SD males, IFFA CREDO, 200-225 g) and to the receptors of guinea pig brain membranes (males, COB LABO, 325-350 g), according to the protocol described by INNIS R. B. and SNYDER S. M., Euro. J. Pharmacol., 65, 123-124, 1980.
The IC.sub.50 values found for the receptors of rat pancreas plasma membranes were, for the majority of the compounds, between 0.01 and 60 nM/l, and the IC.sub.50 values found for the receptors of guinea pig brain membranes between 350 and 30,000 nM/l. The ratios of the IC.sub.50 in relation to the pancreatic receptors to the IC.sub.50 in relation to the brain receptors were found to be between 3 and 20,000.
EXAMPLE 67
Activity in respect of cholecystokinin in vivo
The antagonist activity of the compounds of formula (I) in respect of CCK was determined in mice (SWISS male, 18-20 g) in the gastric evacuation model described by LOTTI V. J. et al. (LIFE SCIENCES, 39; 1631-1638; 1986), by determining the 50% effective dose (ED.sub.50 ) which protects the animals against the inhibition of gastric evacuation induced by sulfated CCK-8 (80 .mu.g/kg, s.c.). The values obtained in the gastric evacuation test were 0.032-0.290 mg/kg.
EXAMPLE 68
Acute hypoxia in mice
Male CDl mice (Charles RIVER) which have received the test compound intraperitoneally 30 minutes beforehand are subjected to an acute hypoxia of the hypobaric type. For this purpose, they are placed in a chamber in which the atmospheric pressure can be lowered rapidly (in the space of 30 seconds) to a value of 160 mbar, thereby causing the death of all the animals approximately 15 seconds after this hypoxic pressure is obtained.
Survival of the brain is assessed by measuring the time of appearance of the last gasp of breath. The survival time of a treated batch is compared with that of a control batch receiving only the solvent.
The results of this study demonstrated that the compounds of the invention are active at a dose of 3 mg/kg and above. In this case, an approximately 30-40% increase in the survival of the animals is observed. For a dose of 100 mg/kg, some compounds of the invention increase the survival time of the animals up to 200%.
EXAMPLE 69
______________________________________Hard gelatin capsules containing 5 mg of 5,6-di-hydro-6-[2-hydroxy-2-(4-methylphenyl)ethyl]-4-oxo-4H-pyrrolo[1,2-a]thieno[3,2-f][1,4]diazepine(compound ofExample 16):______________________________________Compound of Example 16 5 mgCorn starch 20 mgLactose 30 mgTalc 10 mgPer No. 3 hard gelatin capsule.______________________________________

Pyrrolo [1,2-a] thieno [3,2-f] (1,4) diazepines

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Novel Convenient Synthesis of 1,4-Diazepines, 6-Alkoxy-5,6-Dihydro-4H-Pyrrolo [1,2-a] Thieno [3,2-f]-1,4-Diazepine-4-ones, S. Rault et al., Heterocycles (1979) 12 (8) pp. 1009-1011.
Pyrrolo [1,2-a] Thieno [3,2-f]-1,4-Diazepines. Novel Synthesis and X-Ray Analysis, S. Rault, et al., Tetrahedron Letters (1979)N.degree. 7 pp. 643-644.
Synthesis of 4H-Pyrrolo [1,2-a] Thieno [3,2-f] [1,4] Diazepines, H. Fujimori, et al., Journal of Heterocyclic Chemistry (1977) 14 (2) pp. 235-240.
Synthese et etude de la 4H-Dihydro-5,6 Pyrrolo [1,2-a] Thieno [3,2-f] Diazepine-1,4, Comptes rendus de l'Academie des Science de Paris (1978) 287 pp. 117-120.