The disclosure relates to a quality analysis nanosensor using a metastructure, and more particularly to a quality analysis nanosensor based on a metamaterial, in which detection sensitivity is efficiently raised to a high level with only few nanoparticles.
Biosensing technology refers to analysis technology based on a biosensor. To systematically explain the biosensing technology, it is necessary to look at what the biosensor includes. The biosensor largely includes a transducer and a biological element, in which the transducer detects variations in ions, electrons, heat, mass, and light, resulting from a selective reaction between the biological element and an analyte, coverts the variations into electric signals, and amplifies the electric signals into reaction signals. Therefore, depending on the characteristics of the transducer, the biosensor is broadly classified into an ‘electrochemical biosensor’ for detecting variations in electrical properties, an ‘optoelectronic biosensor’ for detecting variations in optical properties, an ‘piezoelectric biosensor’ for detecting variations in mass, and a ‘biothermistor’ for detecting thermal variations in resulting from a bioreaction.
The biosensors have been mainly applied to the fields of medicine, food & agriculture, processes, environments, and the like. A biosensor market size is rapidly growing in the field of food, and the use of the biosensor in the food industry is also expected to increase in the future. In terms of technology, the electrochemical biosensor has the highest share.
In the food industry, the biosensing technology is applicable to the fields such as ingredient analysis, rapid detection of natural toxins and antinutrients, detection of enzyme inactivation and microbial contamination during food processing and food preservation, measurement of hazardous substances generated during a cooking process or by interaction between food ingredients, production of food raw ingredient, analysis of contaminants mixed during processing, measurement of fish freshness, evaluation of antioxidant activity or the like functionality, and fermentation monitoring.
In addition, a biosensor for assessing the freshness by measuring relative proportions of major substances produced while fish meat and livestock meat are decomposing, a biosensor for evaluating antioxidant activity or the like functionality, a biosensor for accessing a food process and measuring the concentration of fermentation products online in real time, etc., are highly applicable in the food industry.
As the biosensor market size is rapidly growing in the field of food, it can be said that the future of food biosensing technology is very bright. Further, the development of proteomics and the like omics technology is promoting the research, development and application of food biosensors.
In the future, there will be a surge in demand for a disposable biosensor or a simple, cost-effective, quick-response and easy-to-use biosensor device. Accordingly, the standardization and miniaturization of biosensor chips are essential to improve reproducibility and reduce costs. Ultimately, it is necessary to develop the biosensing technology for food based on micro-total analysis systems (μTAS) and establish peripheral element technology for this.
In the case of a conventional metamaterial using a nanogap, a nanogap-based metamaterial sensor could be used as a more sensitive sensor due to a field enhancement (FE) effect at the nanogap.
However, it is difficult for the current level of technology to actually apply the nanogap to a low-cost sensor because a manufacturing process is complicated and costs high.
When nanoparticles are bound onto the metamaterial, detection sensitivity is significantly amplified, but there are difficulties such as inefficiency in the case of a big unit cell of metamaterials, and necessity of many nanoparticles.
Further, when simple label-free measurement is performed without a biochemical selective binding site even though the metamaterial is used, detection is possible but inefficient.
The disclosure is conceived to solve the foregoing problems, and an aspect of the disclosure is to provide a detection structure and method based on metamaterials and nanoparticles, which enable efficient detection with only few nanoparticles while raising detection sensitivity to a high level.
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
According to an aspect of the disclosure, there is provided a quality analysis nanosensor using a metastructure, including: a metasurface structure resonating with a specific frequency of incident electromagnetic waves; a fixed binding body formed on a surface of the metasurface structure or inside the metasurface structure on a hotspot area; a movable binding body coupled to the fixed binding body by an attractive force; and a receptor or nanoparticles linked to the movable binding body.
Further, the hotspot area may include an area where a field enhancement phenomenon for strongly concentrating intensity of an electric field occurs.
Further, the fixed binding body may include first magnetic particles including one selected from the group consisting of ferromagnetic metals such as nickel, iron, cobalt, and rare earth compounds, or a mixture thereof, and the movable binding body may include second magnetic particles employing one selected from the group consisting of ferromagnetic metals such as nickel, iron, cobalt, and rare earth compounds, or a mixture thereof; or magnetoplasmonic particles obtained by combining one selected from the group consisting of ferromagnetic metals or a mixture thereof with silver or gold nanoparticles, and bound to the first magnetic particles by an attractive force.
Further, the fixed binding body may include a chemical linker including single, double or multiple ionic ligands with derivatives of sulfur (S), nitrogen (N), and oxygen (O) and the movable binding body may include particles employing metal or nonmetal nanoparticles combined with one or more selected from the group consisting of carbohydrate, peptide, protein, enzyme, lipid, amino acid, deoxyribonucleic acid (DNA), ribonucleic acid (RNA), antibody, polyethylene glycol (PEG), drug, and fluorescent dye, and bound to the chemical linker.
Further, the chemical linker is formed on the surface of the structure or inside the structure on the hotspot area by lithography.
Further, the receptor may be formed with a binding site to which a target material for detecting the quality of an analyte is specifically bound.
The disclosure relates to a quality analysis nanosensor using a metastructure, which includes a metasurface structure resonating with a specific frequency of incident electromagnetic waves; a fixed binding body formed on a surface of the metasurface structure or inside the metasurface structure on a hotspot area; a movable binding body coupled to the fixed binding body by an attractive force; and a receptor or nanoparticles linked to the movable binding body.
Below, the disclosure will be described in detail with reference to the accompanying drawings.
a metasurface structure 10 that resonates with a specific frequency of incident electromagnetic waves;
a fixed binding body 20 formed on a surface of the metasurface structure 10 or inside the structure on a hotspot area;
a movable binding body 30 coupled to the fixed binding body 20 by an attractive force; and
a receptor 40 or nanoparticles linked to the movable binding body 30.
In a metamaterial unit cell, a position of a hotspot, where a field effect (FE) occurs, is varied depending on the structures.
For example, in the case of a resonance structure of a representative split ring resonator such as an electric-field coupled inductor-capacitor (ELC) resonator shown in
In the metastructure sensor according to an embodiment of the disclosure, a plane, on which meta patterns are formed, i.e., the metasurface structure 10 is used as a base, and first magnetic particles 20 are formed on the pattern plane or at specific position inside the pattern, thereby improving detection sensitivity.
Then, the movable binding body 30, i.e., second magnetic particles, which includes a magnetic metal or the like, may be introduced onto the metamaterial surface in the form of flowing as contained in a fluid. The second magnetic particles 30, i.e., the magnetic metal may be used in the form of nanoparticles. In this way, when the magnetic nanoparticles are mixed into the fluid and flow on the surface of the metasurface structure 10, the magnetic nanoparticles are highly likely to be collected near the hotspot selectively formed in the surface of the metasurface structure 10.
The second magnetic particles 30 are linked to the receptor 40 or the nanoparticles.
In this case, the receptor 40 or the nanoparticles are formed with a binding site 41 to be specifically bound to a target material T, and thus a specific target material T for detecting the material quality is bound to the binding site 41. Therefore, all the nanomagnetic particles in the fluid are concentrated and attached to the magnetic pattern of the hotspot area with little loss, and the number of binding sites per unit area of the fixed binding body increases, thereby enhancing the sensitivity. Accordingly, the target material T attached to the binding site 41 of the receptor 40 or nanoparticles is positioned within the hotspot area, thereby greatly improving an efficiency of detecting the quality of the analyte.
In this case, when the second magnetic particles 30 have a dual function of magnetoplasmonic particles combined with nanoparticles of gold, silver or the like, stronger adsorption occurs, thereby enabling high-sensitivity measurement.
The detection mechanism of the foregoing embodiment is similar to that of an embodiment employing the first magnetic particles and the second magnetic particles, in which the target material T specifically bound to the binding site of the receptor or nanoparticles linked to the movable binding body by combination between the chemical linker and the movable binding body is concentrated in a specific hotspot area, thereby improving the detection sensitivity.
Below, embodiments of the disclosure will be described in detail.
To examine these results in more detail,
From such results, the magnetic pattern is formed in the hotspot area regardless of the area of the metamaterial unit cell so that the magnetic particles for the detection can be concentrated in a specific area, thereby enabling highly sensitive measurement with only the biosensor attached to few magnetic particles.
Further, stronger adsorption occurs when the magnetoplasmonic particles are used as the second magnetic particles, thereby further amplifying the sensitivity.
10: metasurface structure
20: fixed binding body
30: movable binding body
40: receptor
41: binding site
T: target material
M: first magnetic particles
L: chemical linker
According to the disclosure, there is provided a nanosensor for detecting the quality, which has a detection structure based on metamaterials and nanoparticles, thereby enabling efficient detection with only few nanoparticles by raising detection sensitivity to a high level.
Number | Date | Country | Kind |
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10-2020-0050916 | Apr 2020 | KR | national |
Number | Date | Country | |
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Parent | PCT/KR2021/005292 | Apr 2021 | US |
Child | 17972957 | US |