Claims
- 1. In an immunoassay employing an immunoassay reagent comprising liposomes made from phospholipids, glycolipids or a mixture thereof, a marker material encapsulated in said liposomes, and an antibody, antibody fragment, or an antigen, immobilized on said liposomes by a cross-linking method using a cross-linker comprising a dithiopyridyl group, wherein said immunoassay method comprises the steps of
- (a) adding a sample to said liposomes in the presence of complement, and
- (b) determining the extent to which said marker is released,
- the improvement wherein in step (a), said addition of sample to said liposomes is effected in the further presence of a disulfide-reducing material that cleaves the disulfide bond of said dithiopyridyl group, whereby non-specific lysis of said liposomes is prevented.
- 2. The immunoassay according to claim 1, wherein said liposomes further comprise cholesterol.
- 3. The immunoassay according to claim 1, wherein said disulfide-reducing material is 2-mercaptoethanol.
- 4. The immunoassay according to claim 1, wherein said disulfide-reducing material is cysteine.
Priority Claims (2)
Number |
Date |
Country |
Kind |
58-224509 |
Nov 1983 |
JPX |
|
62-34480 |
Feb 1987 |
JPX |
|
Parent Case Info
This is a continuation in part application of our U.S. Ser. No. 675,607 filed Nov. 28, 1984, now abandoned.
Foreign Referenced Citations (1)
Number |
Date |
Country |
8302069 |
Jun 1983 |
WOX |
Non-Patent Literature Citations (1)
Entry |
Hashimoto et al., "Coating of Liposomes with Subunits . . . " Journal of Immunological Methods 62 (1983), pp. 155-162. |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
675607 |
Nov 1984 |
|