Claims
- 1. An isolated nucleic acid comprising a member selected from the group consisting of:
(a) a polynucleotide having at least 70% sequence identity over the entire length of the polynucleotide of SEQ ID NO:1, as determined by the GAP algorithm under default parameters; (b) a polynucleotide encoding the polypeptide of SEQ ID NO:2; (c) a polynucleotide amplified from a Zea mays nucleic acid library using primers which selectively hybridize, under stringent hybridization conditions, to loci within the polynucleotide of SEQ ID NO:1; (d) a polynucleotide which selectively hybridizes, under stringent hybridization conditions and a wash in 1×SSC at 60° C., to the polynucleotide of SEQ ID NO:1; (e) the polynucleotide of SEQ ID NO:1; (f) a polynucleotide which is complementary to a polynucleotide of (a), (b), (c), (d), or (e); and (g) a polynucleotide comprising at least 300 contiguous nucleotides from a polynucleotide of (a), (b), (c), (d), (e), or (f).
- 2. A recombinant expression cassette, comprising a member of claim 1 operably linked, in sense or anti-sense orientation, to a promoter.
- 3. A non-human host cell comprising the recombinant expression cassette of claim 2.
- 4. A host cell of claim 3, wherein the host cell is a plant cell.
- 5. A transgenic plant comprising a recombinant expression cassette of claim 2.
- 6. The transgenic plant of claim 5, wherein said plant is a monocot.
- 7. The transgenic plant of claim 5, wherein said plant is a dicot.
- 8. The transgenic plant of claim 5, wherein said plant is selected from the group consisting of: maize, soybean, safflower, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, and millet.
- 9. A transgenic seed from the transgenic plant of claim 5.
- 10. A method of modulating the level of Rad3 in a plant, comprising:
(a) introducing into a plant cell a recombinant expression cassette comprising a Rad3 polynucleotide of claim 1 operably linked to a promoter; (b) culturing the plant cell under plant cell growing conditions; and (c) inducing expression of said polynucleotide for a time sufficient to modulate the level of Rad3 in said plant.
- 11. The method of claim 10, wherein the plant is maize.
- 12. An isolated protein comprising a member selected from the group consisting of:
(a) a polypeptide of at least 30 contiguous amino acids from the polypeptide of SEQ ID NO:2; (b) the polypeptide of SEQ ID NO:2; (c) a polypeptide having at least 80% sequence identity to, and having at least one linear epitope in common with, the polypeptide of SEQ ID NO:2; and (d) at least one polypeptide encoded by a member of claim 1.
- 13. A method for increasing transformation efficiency comprising introducing at least one RAD3 polypeptide or at least one Rad3 polynucleotide and a polynucleotide of interest into a responsive host cell to produce a transformed cell and growing the transformed cell under cell growing conditions, wherein the polynucleotides are each operably linked to a promoter.
- 14. The method of claim 13, wherein the host cell is from a plant.
- 15. The method of claim 13, wherein the plant cell is from a monocot or a dicot.
- 16. The transgenic plant of claim 13 wherein said plant is selected form the group consisting of: maize, soybean, safflower, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, and millet.
- 17. The method of claim 13, wherein the plant cell is a maize cell.
- 18. A method of producing male sterility or seedless fruit in a plant, comprising the method of claim 10, using an appropriate promoter under developmental control.
- 19. The transgenic plant of claim 18.
- 20. The transgenic plant of claim 19 wherein said plant is a monocot or a dicot.
- 21. The transgenic plant of claim 19 wherein said plant is selected from the group consisting of: maize, soybean, safflower, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, and millet.
- 22. The transgenic seed from the transgenic plant of claim 19.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. application Ser. No. 60/170,597 filed Dec. 13,1999, which is herein incorporated in entirety by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60170597 |
Dec 1999 |
US |