Claims
- 1. An isolated protein comprising a polypeptide selected from the group consisting of:
a) a polypeptide having at least 90% sequence identity to a polypeptide of SEQ ID NO: 3 or SEQ ID NO: 7, wherein the percent sequence identity is determined over the entire reference sequence using the GAP program under default parameters; b) a polypeptide encoded by SEQ ID NO: 1 or SEQ ID NO: 5; c) a polypeptide of SEQ ID NO: 3 or SEQ ID NO: 7; and d) a polypeptide comprising at least 60 contiguous amino acids from the polypeptide of (a), (b) or (c); wherein the polypeptide of (a), (b), or (c) has recombinase activity.
- 2. A recombinant expression cassette comprising a polynucleotide operably linked to a promoter, wherein the polynucleotide encodes the polypeptide of claim 1.
- 3. A transformed host cell comprising the isolated polypeptide of claim 1.
- 4. The host cell of claim 3, wherein the host cell is a transformed plant cell.
- 5. The plant cell of claim 4, wherein the plant cell is selected from the group consisting of sorghum, maize, rice, wheat, soybean, sunflower, canola, alfalfa, barley, and millet.
- 6. A transformed plant regenerated from the plant cell of claim 4.
- 7. The plant of claim 6, wherein the plant is selected from the group consisting of sorghum, maize, rice, wheat, soybean, sunflower, canola, alfalfa, barley, and millet.
- 8. A transformed seed of the plant of claim 6.
- 9. The polypeptide of claim 1, wherein the polypeptide has at least 95% sequence identity to the polypeptide of SEQ ID NO: 3 or SEQ ID NO: 7.
- 10. A nuclear localization sequence comprising the first 10 to 40 amino acids of SEQ ID NO: 3 or SEQ ID NO: 7.
- 11. The nuclear localization sequence of claim 10, wherein the nuclear localization sequence comprises amino acids 1 to 20 of SEQ ID NO: 3, or amino acids 1 to 20 of SEQ ID NO: 7.
- 12. An isolated protein comprising the nuclear localization sequence of claim 10 operably linked to a polypeptide of interest, wherein the polypeptide of interest is thereby localized to the nucleus of a plant cell.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application is a divisional of co-pending U.S. application Ser. No. 09/246,963 filed Feb. 9, 1999, and claims the benefit of U.S. application Ser. No. 60/074,745 filed Feb. 13, 1998, all of which are herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60074745 |
Feb 1998 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09246963 |
Feb 1999 |
US |
Child |
10246581 |
Sep 2002 |
US |