RAPID PRODUCTION OF HOMOZYGOUS TRANSGENIC ANIMALS

Information

  • Research Project
  • 6645311
  • ApplicationId
    6645311
  • Core Project Number
    R43GM068390
  • Full Project Number
    1R43GM068390-01
  • Serial Number
    68390
  • FOA Number
  • Sub Project Id
  • Project Start Date
    6/15/2003 - 21 years ago
  • Project End Date
    12/14/2004 - 20 years ago
  • Program Officer Name
    GRIEDER, FRANZISKA B
  • Budget Start Date
    6/15/2003 - 21 years ago
  • Budget End Date
    12/14/2004 - 20 years ago
  • Fiscal Year
    2003
  • Support Year
    1
  • Suffix
  • Award Notice Date
    6/9/2003 - 21 years ago
Organizations

RAPID PRODUCTION OF HOMOZYGOUS TRANSGENIC ANIMALS

DESCRIPTION (provided by applicant): The objective of this proposal is to produce homozygous cell lines from existing hemizygous transgenic goats and cows, for use in producing homozygous transgenic animals. GTC Biotherapeutics (formerly Genzyme Transgenics Corp) has developed a safe, abundant and cost effective production source for recombinant biopharmaceuticals by expressing recombinant proteins in the milk of transgenic dairy animals. Current transgenic methods produce first generation hemizygous animals that carry the transgene(s) at a single chromosomal integration site. Doubling the transgene copy number by producing homozygous animals would double protein productivity and simplify future breeding schemes. However, the only existing way to generate homozygotes is through two generations of progeny testing. We have devised a strategy for rapidly selecting homozygous primary cell lines that are derived from the original transgenic animals and that can be used as nuclear transfer donors to produce homozygous transgenic animals. We now propose to establish the feasibility of this strategy by: 1) optimizing methods for producing homozygous fibroblast cell lines; 2) characterizing the cell lines to confirm the presence of one transgene copy on each of two homologues; and 3) producing homozygous transgenic embryos by nuclear transfer. If this approach is successful, it will cost -effectively boost transgenic productivity.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R43
  • Administering IC
    GM
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    100000
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
    NIGMS:100000\
  • Funding Mechanism
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    GTC BIOTHERAPEUTICS, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    FRAMINGHAM
  • Organization State
    MA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    01702
  • Organization District
    UNITED STATES