Recombinant herpesvirus of turkeys and uses thereof

BACKGROUND OF THE INVENTION
The ability to isolate DNA and clone this isolated DNA into bacterial plasmids has greatly expanded the approaches available to make viral vaccines. The methods used to make the present invention involve modifying cloned DNA sequences from various pathogens of animals, by insertions, deletions, single or multiple base changes, and subsequent insertions of these modified sequences into the genome of the virus. One utility of the addition of foreign sequences is achieved when the foreign sequence encodes a foreign protein that is expressed during viral infection of the animal. The resulting live virus may then be used in a vaccine to elicit an immune response in a host animal and provide protection to the animal against disease. A virus with these characteristics is referred to as a viral vector, because it becomes a living vector that will carry and express the foreign protein in the host animal. In effect it becomes an elaborate delivery system for the foreign protein(s).
More specifically, the present invention relates to the use of herpesvirus of turkeys (HVT) as a viral vector for vaccination of birds against disease. The group of herpesviruses comprise various pathogenic agents that infect and cause disease in a number of target species: swine, cattle, chickens, horses, dogs, cats, etc. Each herpesvirus is specific for its host species, but they are all related in the structure of their genomes, their mode of replication, and to some extent in the pathology they cause in the host animal and in the mechanism of the host immune response to the virus infection.
The application of recombinant DNA techniques to animal viruses has a relatively recent history from about 1990. The first viruses to be engineered have been those with the smallest genomes. In the case of the papovaviruses, because these viruses are so small and cannot accommodate much extra DNA, their entire use in genetic engineering has been as defective replicons. Foreign gene expression from these viruses requires a wild-type helper virus and is limited to cell culture systems. For adenoviruses, there is a small amount of nonessential DNA that can be replaced by foreign sequences. The only foreign DNA that seems to have been expressed in adenoviruses are the T-antigen genes from papovaviruses (Mansour, et al., Proc. Natl. Acad. Sci. US, 1985; Thummel, et al., Cell, 1983; Scolnick, et al., Cell, 1981; Thummel, et al., Cell, 1981), and the herpes simplex virus (HSV) thymidine kinase gene (Haj-Ahmed and Graham, J. of Virology, 1986). These publications do not identify the nonessential regions in HVT wherein foreign DNA may be inserted, nor do they teach how to achieve the expression of the foreign genes in HVT, e.g., which promoter sequence and termination sequence to use.
Another group of viruses that have been engineered are the poxviruses. One member of this group, vaccinia, has been the subject of much research on foreign gene expression. Poxviruses are large DNA-containing viruses that replicate in the cytoplasm of the infected cell. They have a structure that is unique in that they do not contain any capsid that is based upon icosahedral symmetry or helical symmetry. The poxviruses are most likely to have evolved from bacterial-like microorganisms through the loss of function and degeneration. In part due to this uniqueness, the advances made in the genetic engineering of poxviruses cannot be directly extrapolated to other viral systems, including herpesviruses and HVT. Vaccinia recombinant virus constructs have been made in a number of laboratories that express the following inserted foreign genes: HSV thymidine kinase gene (Mackett, et al., Proc. Natl. Acad. Sci. USA, 1982; Panicali and Paoletti, Proc. Natl. Acad. Sci. USA, 1982, hepatitis B surface antigen (Paoletti, et al., Proc. Natl. Acad. Sci. USA, 1984; Smith et al., Nature, 1983), HSV glycoprotein D gene, influenzae hemagglutinin gene (Panicali, et al., Proc. Natl. Acad. Sci. USA, 1983; Smith, et al., Proc. Natl. Acad. Sci. USA, 1983), malaria antigen gene (Smith, et al., Science, 1984, and vesicular stomatitis glycoprotein G gent (Mackett, et al., Science, 1986). The general overall features of vaccinia recombinant DNA work are similar to the techniques used for all the viruses, especially as they relate to the techniques in reference (Maniatis, et al., Molecular Cloning, 1982). However in detail, the vaccinia techniques are not applicable to herpesviruses and HVT. The utility of vaccinia as a vaccine vector is in question because of its close relationship to human smallpox and its known pathogenicity to humans. Thus, the use of the host-specific herpesvirus HVT is a better solution to vaccination of poultry.
Among the primate herpesviruses, only HSV of humans and, to a limited extent, herpes saimiri of monkeys have been engineered to contain foreign DNA sequences. The first use of recombinant DNA to manipulate HSV involved cloning a piece of DNA from the L-S junction region into the unique long region of HSV DNA, specifically into the thymidine kinase gene (Moccarski, et al., Cell, 1980). This insert was not a foreign piece of DNA, rather it was a naturally occurring piece of herpesvirus DNA that was duplicated at another place in the genome. This piece of DNA was not engineered to specifically express a protein, and thus the work does not involve expression of protein in herpesviruses. The next manipulation of HSV involved the creation of deletions in the virus genome by a combination of recombinant DNA techniques and thymidine kinase selection. Using this approach, the HSV alpha-22 gene has been deleted (Post, et al., Cell, 1981), and a 15,000 basepair sequence of DNA has been deleted from the internal repeat of HSV (Poffenberger, et al., Proc. Natl. Acad. Sci. USA, 1981).
The insertion of genes that encode protein into herpesviruses have involved seven cases: the insertion of HSV glycoprotein C back into a naturally occurring deletion mutant of this gene in HSV (Gibson and Spear, J. of Virology, 1983); the insertion of glycoprotein D of HSV type 2 into HSV type 1 (Lee, et al., Proc. Natl. Acad. Sci. USA, 1982), again with no manipulation of promoter sequences since the gene is not `foreign`; the insertion of hepatitis B surface antigen into HSV under the control of the HSV ICP4 promoter (Shih, et al., Proc. Natl. Acad. Sci. USA, 1984); and the insertion of bovine growth hormone into herpes saimiri virus with an SV40 promoter (the promoter did not work this system and an endogenous upstream served to transcribe the gene) (Desrosiers, et al., 1984). Two additional cases of foreign genes (chicken ovalbumin gene and Epstein-Barr virus nuclear antigen) have been inserted into HSV (Arsenakis and Roizman, 1984), and glycoprotein X of pseudorabies virus has been inserted into HSV (Post, et al., 1985).
These limited cases of deletion and insertion of genes into herpesviruses demonstrate that it is possible to genetically engineer herpesvirus genomes by recombinant DNA techniques. The methods that have been used to insert genes involve homologous recombination between the viral DNA cloned in plasmids and purified viral DNA transfected into the same animal cell. However, the extent to which one can generalize the location of the deletion and the sites for insertion of foreign genes is not known from these previous studies.
One object of the present invention is a vaccine for Marek's disease. Marek's disease virus (MDV) is the causative agent of Marek's disease which encompasses fowl paralysis, a common lymphoproliferative disease of chickens. The disease occurs most commonly in young chickens between 2 and 5 months of age. The prominent clinical signs are progressive paralysis of one or more of the extremities, incoordination due to paralysis of legs, drooping of the limb due to wing involvement, and a lowered head position due to involvement of the neck muscles. In acute cases, severe depression may result. In the case of highly oncogenic strains, there is characteristic bursal and thymic atrophy. In addition, there are lymphoid tumors affecting the gonads, lungs, liver, spleen, kidney and thymus (Mohanty and Dutta, 1981).
Most chickens are vaccinated against MDV at one day of age to protect the bird against MDV for life. Prior to the present invention, the principal vaccination method for MDV involved using naturally occurring strains of turkey herpesvirus (HVT). It would be advantageous to incorporate other antigens into this vaccination at one day of age, but efforts to combine conventional vaccines have not proven satisfactory to date due to competition and immunosuppression between pathogens. The multivalent HVT-based vaccines engineered in this invention represent a novel way to simultaneously vaccinate against a number of different pathogens. For the first time, a recombinant HVT with a foreign gene inserted into a non-essential region of the HVT genome is disclosed.
The types of genetic engineering that have been performed on these herpesviruses consist of cloning parts of the virus DNA into plasmids in bacteria, reconstructuring the virus DNA while in the cloned state so that the DNA contains deletions of certain sequences, and furthermore adding foreign DNA sequences either in place of the deletions or at sites removed from the deletions.
A foreign gene of interest targeted for insertion into the genome of HVT may be obtained from any pathogenic organism of interest. Typically, the gene of interest will be derived from pathogens that cause diseases in poultry that have an economic impact on the poultry industry. The genes may be derived from organisms for which there are existing vaccines, and because of the novel advantages of the vectoring technology the HVT derived vaccines will be superior. Also, the gene of interest may be derived from pathogens for which there is currently no vaccine but where there is a requirement for control of the disease. Typically, the gene of interest encodes immunogenic polypeptides of the pathogen, and may represent surface proteins, secreted proteins and structural proteins.
A relevant avian pathogen that is a target for HVT vectoring in the present invention is Infectious Laryngotracheitis virus (ILT). ILT is a member of the herpesviridiae family, and this pathogen is causes an acute disease of chickens which is characterized by respiratory depression, gasping and expectoration of bloody exudate. Viral replication is limited to cells of the respiratory tract, where in the trachea the infection gives rise to tissue erosion and hemorrhage. In chickens, no drug has been effective in reducing the degree of lesion formation or in decreasing clinical signs. Vaccination of birds with various modified forms of the ILT virus derived by cell passage and/or tedious regimes of administration have conferred acceptable protection in susceptible chickens. Because of the degree of attenuation of current ILT vaccines care must be taken to assure that the correct level of virus is maintained; enough to provide protection, but not enough to cause disease in the flock.
An additional target for the HVT vectoring approach and a subject of this invention is Newcastle disease, an infectious, highly contagious and debilitating disease that is caused by the Newcastle disease virus (NDV). NDV is a single-stranded RNA virus of the paramyxovirus family. The various pathotypes of NDV (velogenic, mesogenic, lentogenic) differ with regard to the severity of the disease, the specificity and symptoms, but most types seem to infect the respiratory system and the nervous system. NDV primarily infects chickens, turkeys and other avian species. Historically vaccination has been used to prevent disease, but because of maternal antibody interfences, life-span of the bird and route of administration, the producer needs to adapt immunization protocols to fit specific needs.
SUMMARY OF THE INVENTION
The present invention provides a recombinant herpesvirus of turkeys (HVT) which comprises the genomic DNA of herpesvirus of turkey containing foreign DNA not usually present within such genomic DNA. The foreign DNA is inserted within a region of the genomic DNA which is not essential for replication of the herpesvirus of turkeys and DNA corresponding to a promoter is located upstream of the said foreign DNA.
The invention provides for a homology vector for producing a recombinant herpesvirus of turkeys by inserting foreign DNA into the genomic DNA of a herpesvirus of turkeys. The homology vector comprises a double-stranded DNA molecule consisting essentially of a double-stranded foreign DNA not usually present within the herpesvirus of turkeys genomic DNA, with at one end of the foreign DNA, double-stranded herpesvirus of turkeys DNA homologous to genomic DNA located at one side of a site on the genomic DNA which is not essential for replication of the herpesvirus of turkeys, and at the other end of the foreign DNA, double-stranded herpesvirus of turkeys DNA homologous to genomic DNA located at the other side of the same site on the genomic DNA.

BRIEF DESCRIPTION OF FIGURES
FIGS. 1A-1C
Details of HVT Construction and Map Data
A. BamHI restriction fragment map of HVT. Fragments are numbered in order of decreasing size; letters refer to small fragments whose comparative size has not been determined.
B. BamHI #16 fragment showing location of .beta.-galactosidase gene insertion in S-HVT-001.
C. BamHI #19 fragment showing location of .beta.-galactosidase gene insertion.
Legend: B=BamHI; X=XhoI; H=HindIII; P=PstI; S=SalI; N=NdeI; R=EcoRI.
FIG. 2A, FIG. 2B, FIG. 2C and FIG. 2D
Insertion in Plasmid 191-47
FIGS. 3A-3B
Details of S-HVT-003 Construction
A. Restriction map of HVT DNA in the region of the BamHI #16 fragment. This fragment is contained within large HindIII fragment that has no name in the prior art to applicants' knowledge. Shown also is the XhoI site (X) where applicants have made their constructions the XhoI site was first changed to an EcoRI (R) site by use of a "linker" and standard cloning procedures. Also, shows details of the construction of the beta-gal gene and IBVD gene inserted into the BamHI #16 fragment for use in homologous recombination. Both genes were under the control of the PRV gX gene promoter (gX).
B. The S-HVT-003 genome showing the location of the two inserted foreign genes, .beta.-gal and IBDV.
Legend: H=HindIII; B=BamHI; X=XhoI; R=EcoRI; Xb=XbaI; Hp=HpaI; S=SmaI; UL=unique long region; US=unique short region; IR=internal repeat region; TR=terminal repeat region.
FIG. 4
Western blot showing the differential expression of the IBDV 32 kD antigen in cellular lysates of S-HVT-003 infected cells (32 kD present) and S-HVT-001 infected cells (32 kD negative). IBDV specific polypeptides were identified by probing the blot with hyper-immune rat antiserum directed against denatured IBDV virions. This serum reacts primarily with the immunodominant 32 kD antigen (VP3). The lanes on the blot contain: 1) Protein Molecular Weight Standards, 2) uninfected CEF cells, 3) S-HVT-001 infected CEF's, 4) 5) & 6) S-HVT-003 and 7) IBDV virion polypeptides.
FIG. 5
Western blot showing the presence of the 42 kD IBDV polypeptide (VP2) product in cellular lysates derived from CEF cells infected with either wild-type HVT virus or with S-HVT-001 that expresses .beta.-galactosidase. IBDV specific polypeptides were identified using a VP-2 specific rabbit anti-peptide antiserum. The lanes contain: 1) Protein Molecular Weight Standards, 2) wild-type HVT infected CEF's, 3) S-HVT-001 infected CEF's, 4) S-HVT-003 infected CEF's, 5) S-HVT-003 infected CEF's, and 6) IBDV virion polypeptides.
FIGS. 6A-6C
Details of S-HVT-004 Construction
A. Restriction map of HVT DNA in the region of the BamHI #16 fragment. This fragment is contained within a large HindIII fragment that has no name in the prior art to applicants' knowledge. Shown also is the XhoI site (X) where applicants have made their insertion: for these constructions the XhoI site was first changed to an EcoRI (R) site by use of a "linker" and standard cloning procedures.
B. Details of the construction of the .beta.-gal gene and MDV gA gene inserted into the BamHI #16 fragment for use in homologous recombination. Beta-gal was under the control of the PRV gX gene promoter (gX), while the MDV gA gene was under the control of its own promoter.
C. The S-HVT-004 genome showing the location of the two inserted foreign genes, .beta.-gal and MDV gA.
Legend: H=HindIII; B=BamHI; X=XhoI; R=EcoRI; Xb=XbaI; UL=unique long region; US=unique short region; IR=internal repeat region; TR=terminal repeat region.
FIG. 7A and FIG. 7B
FIG. 7 is a detailed description of the .beta.-galactosidase (lacZ) marker gene insertion in homology vector 467-22.A12. The diagram shows the orientation of DNA fragments assembled in the marker gene. The origin of each fragment is described in the Materials and Methods section. The sequences located at the junctions between each fragment and at the ends of the marker gene are shown. The restriction sites used to generate each fragment are indicated at the appropriate junction. The location of the lacZ gene coding region is also given. Numbers in parenthesis ( ) refer to amino acids, and restriction sites in brackets � ! indicate the remnants of sites which were destroyed during construction. The following abbreviations are used, pseudorabies virus (PRV), lactose operon Z gene (lacZ), Escherichia coil (E. Coli), poly adenylation signal (pA), and glycoprotein X (gpx).
FIG. 8
FIG. 8 is a BamHI, NotI restriction map of the HVT genome. The unique long UL and unique short US regions are shown. The long and short region repeats are indicated by boxes. The BamHI fragments are numbered in decreasing order of size. The location of probes P1-P4 are indicated. The origin of each probe is as follows: P1--BamHI #6, P2--BamHI #2, P3--BamHI #13, and P4--4.0 kb BgIII to StuI sub-fragment of HVT genomic XbaI fragment #5 (8.0 kb).
FIG. 9
FIG. 9 describes the creation of plasmid pSY229.
FIG. 10A and FIG. 10B
Detailed description of the MDV gene cassette insert in Homology Vectors 456-18.18 and 456-17.22. The diagram shows the orientation of DNA fragments assembled in the cassette. The origin of each fragment is described in the Materials and Methods section. The sequences located at the junctions between each fragment and at the ends of the marker gene are shown, including junction A (SEQ ID NO: 34), junction B (SEQ ID NO: 35), and junction C (SEQ ID NO: 36). The restriction sites used to generate each fragment are indicated at the appropriate junction. The location of the MDV gA and gB genes is also given. Numbers in parenthesis ( ) refer to amino acids, and restriction sites in brackets � ! indicate the remnants of sites which were destroyed during construction.
FIG. 11A and FIG. 11B
Detailed description of the HindIII fragment insert in Homology Vector 556-41.5. The diagram shows the orientation of DNA fragments assembled in the cassette. The origin of each fragment is described in the Materials and Methods section. The sequences located at the junctions between each fragment and at the ends of the marker gene are shown, including junction A (SEQ ID NO: 37), junction B (SEQ ID NO: 38), and junction C (SEQ ID NO: 39). The restriction sites used to generate each fragment are indicated at the appropriate junction. The location of the MDV gD and a portion of the gI gene is also given. Numbers in parenthesis ( ) refer to amino acids, and restriction sites in brackets � ! indicate the remnants of sites which were destroyed during construction.
FIG. 12A, FIG. 12B and FIG. 12C
Detailed description of the SalI fragment insert in Homology Vector 255-18.B16. The diagram shows the orientation of DNA fragments assembled in the cassette. The origin of each fragment is described in the Materials and Methods section. The sequences located at the junctions between each fragment and at the ends of the marker gene are shown, including junction A (SEQ ID NO: 40), junction B (SEQ ID NO: 41), junction C (SEQ ID NO: 42), junction D (SEQ ID NO: 43), junction E (SEQ ID NO: 44), junction F(SEQ ID NO: 45), junction G (SEQ ID NO: 46), and junction H (SEQ ID NO: 47). The restriction sites used to generate each fragment are indicated at the appropriate junction. The location of the NDV F and lacZ-NDV HN hybrid gene are shown. Numbers in parenthesis ( ) refer to amino acids, and restriction sites in brackets � ! indicate the remnants of sites which were destroyed during construction.

DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a recombinant herpesvirus of turkeys (HVT) which comprises the genomic DNA of herpesvirus of turkey containing foreign DNA not usually present within such genomic DNA. The foreign DNA is inserted within a region of the genomic DNA which is not essential for replication of the herpesvirus of turkeys and DNA corresponding to a promoter is located upstream of the said foreign DNA.
For purposes of this invention, "genomic DNA" means the entire DNA which the naturally occurring herpesvirus of turkeys contains. For purposes of this invention, "foreign DNA" means any DNA that is exogenous to the genomic DNA.
For purposes of this invention, a "promoter" is a specific DNA sequence on the DNA molecule to which the foreign RNA polymerase attaches and at which transcription of the foreign RNA is initiated.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA which encodes a polypeptide. Preferably, the polypeptide is antigenic in the animal into which the recombinant HVT is introduced.
Preferably, this antigenic polypeptide is a linear polymer of more than 10 amino acids linked by peptide bonds which stimulates the animal to produce antibodies.
The invention further provides an insertion site present within BamHI #16 fragment of the herpesvirus of turkeys genome. Preferably, the insertion site is within an open reading frame encoding UL43 protein. Preferably, the insertion site is the XhoI restriction endonuclease site located within an open reading frame encoding UL43 protein.
For purposes of this invention, an "open reading frame" is a segment of DNA which contains codons that can be transcribed into RNA which can be translated into an amino acid sequence and which does not contain a termination codon.
The invention further provides an insertion site within EcoRI #9 fragment of the herpesvirus of turkeys genome. Preferably, the insertion site is XhoI restriction endonuclease site located within the EcoRI #9 fragment.
The invention further provides an insertion site within US2 gene coding region of the herpesvirus of turkeys genome. Preferably, the insertion site is StuI restriction endonuclease site located within the US2 gene coding region.
The invention further provides a recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding a polypeptide which is a detectable marker. Preferably, the detectable marker is E. coli beta-galactosidase. For purposes of this invention, a "polypeptide which is a detectable marker" includes the bimer, trimer and tetramer form of the polypeptide. E. coli .beta.-galactosidase is a tetramer composed of four polypeptides or monomer subunits. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-001, S-HVT-014, or S-HVT-012. The S-HVT-012 herpesvirus has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganism for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
The invention further provides a recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide which is from infectious bursal disease virus, Marek's disease virus, Newcastle disease virus, infectious laryngotracheitis virus, or infectious bronchitis virus.
The invention further provides a recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide which is from infectious bursal disease virus. Preferably, antigenic polypeptide is VP2, VP3 or VP4 protein.
The invention further provides a recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide from infectious bursal disease virus and further comprising foreign DNA encoding a polypeptide which is a detectable marker. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-003 or S-HVT-096. The S-HVT-003 herpesvirus has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganism for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide from Marek's disease virus. Preferably, the antigenic polypeptide is Marek's disease virus glycoprotein gB, gA or gD.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gA.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gA and further comprising foreign DNA encoding a polypeptide which is a detectable marker. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-004. The S-HVT-004 herpesvirus has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganism for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A. under ATCC Accession No. VR.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-045. The S-HVT-045 herpesvirus has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganism for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
This invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB and further comprising foreign DNA encoding Marek's disease virus glycoprotein gA. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-046 or S-HVT-047.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB, Marek's disease virus glycoprotein gA, and Marek's disease virus glycoprotein gD. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-048.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide from Newcastle disease virus. Preferably, this antigenic polypeptide is Newcastle disease virus fusion (F) protein or Newcastle disease virus hemagglutinin-neuraminidase (HN) protein.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Newcastle disease virus fusion (F) protein and further comprising foreign DNA encoding a recombinant protein, wherein E. coli beta-galactosidase is fused to Newcastle disease virus hemmagglutinin-neuraminidase (HN) protein. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-007.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide from Marek's disease virus and further comprising foreign DNA encoding antigenic polypeptide from Newcastle disease virus.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB and Marek's disease virus glycoprotein gA and further comprising foreign DNA encoding Newcastle disease virus fusion (F) protein. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-048.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB and Marek's disease virus glycoprotein gA and further comprising foreign DNA encoding Newcastle disease virus hemagglutinin-neuraminidase (HN) protein. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-049.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB and Marek's disease virus glycoprotein gA and further comprising foreign DNA encoding Newcastle disease virus fusion (F) protein and Newcastle disease virus hemagglutinin-neuraminidase (HN) protein. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-050.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB, Marek's disease virus glycoprotein gA, and Marek's disease virus glycoprotein gD and further comprising foreign DNA encoding Newcastle disease virus fusion (F) protein and foreign DNA encoding Newcastle disease virus hemagglutinin-neuraminidase (HN) protein. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-106.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding infectious laryngotracheitis virus glycoprotein gB or infectious laryngotracheitis virus glycoprotein gD. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-051 or S-HVT-052.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide from Marek's disease virus and further comprising foreign DNA encoding antigenic polypeptide from infectious laryngotracheitis virus.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gA and further comprising foreign DNA encoding infectious laryngotracheitis virus glycoprotein gD and foreign DA encoding infectious laryngotracheitis virus glycoprotein gB. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-096.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding Marek's disease virus glycoprotein gB, Marek's disease virus glycoprotein gA, and Marek's disease virus glycoprotein gD and further comprising foreign DNA which encodes infectious laryngotracheitis virus glycoprotein gD, infectious laryngotracheitis virus glycoprotein gB, and E. coli beta-galactosidase. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-104.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding infectious bronchitis virus spike protein or infectious bronchitis virus matrix protein.
The invention further provides recombinant herpesvirus of turkeys whose genomic DNA contains foreign DNA encoding antigenic polypeptide from Marek's disease virus and further comprising foreign DNA encoding infectious bronchitis virus spike protein or infectious bronchitis matrix protein. Preferably, this recombinant herpesvirus of turkeys is designated S-HVT-066.
The inserted foreign DNA is under the control of a promoter. Preferably, the promoter is a herpesvirus promoter. Preferably, the promoter is selected from PRV gX, HSV-1 alpha 4, HCMV immediate early, MDV gB, MDV gD, ILT gB, and ILT gD. For purposes of this invention, the promoters were generated by methods well known to those of skill in the art.
The invention provides for a homology vector for producing a recombinant herpesvirus of turkeys by inserting foreign DNA into the genomic DNA of a herpesvirus of turkeys. The homology vector comprises a double-stranded DNA molecule consisting essentially of a double-stranded foreign DNA not usually present within the herpesvirus of turkeys genomic DNA, with at one end of the foreign DNA, double-stranded herpesvirus of turkeys DNA homologous to genomic DNA located at one side of a site on the genomic DNA which is not essential for replication of the herpesvirus of turkeys, and at the other end of the foreign DNA, double-stranded herpesvirus of turkeys DNA homologous to genomic DNA located at the other side of the same site on the genomic DNA. DNA sequence corresponding to a promoter is located upstream of the foreign DNA and controls the expression of the foreign DNA.
In one embodiment of the invention, the foreign DNA encodes a polypeptide. In one embodiment of the invention, the polypeptide is antigenic in the animal into which the recombinant herpesvirus of turkeys is introduced. Preferably, the antigenic polypeptide is from infectious bursal disease virus, Marek's disease virus, Newcastle disease virus, infectious laryngotracheitis virus, or infectious bronchitis virus. Preferably, the antigenic polypeptide is selected from a group consisting of infectious bursal disease virus VP2 protein, infectious bursal disease virus VP3 protein, infectious bursal disease virus VP4 protein, Marek's disease virus glycoprotein gB, Marek's disease virus glycoprotein gA, Marek's disease virus glycoprotein gD, Newcastle disease virus fusion (F) protein, Newcastle disease virus hemagglutinin-neuraminidase (HN) protein, infectious laryngotracheitis virus glycoprotein gB, infectious laryngotracheitis virus glycoprotein gD, infectious bronchitis virus spike protein, or infectious bronchitis virus matrix protein.
In one embodiment of the invention, the polypeptide is a detectable marker. Preferably, the polypeptide which is a detectable marker is E. coli beta-galactosidase.
In one embodiment of the invention, the double-stranded herpesvirus of turkeys DNA is homologous to DNA sequences present within the BamHI #16 fragment of the herpesvirus of turkeys genome. Preferably, the double-stranded herpesvirus of turkeys DNA is homologous to DNA sequences present within the open reading frame encoding UL 43 protein of the herpesvirus of turkeys genome. Preferably, this homology vector is designated 172-29.31.
For purposes of this invention, a "homology vector" is a plasmid constructed to insert foreign DNA in a specific site on the genome of a herpesvirus of turkeys.
In one embodiment of the invention, the double-stranded herpesvirus of turkeys DNA is homologous to DNA sequences present within the EcoRI #9 fragment of the herpesvirus of turkeys genome. Preferably, this homology vector is designated 172-63.1.
In one embodiment of the invention, the double-stranded herpesvirus of turkeys DNA is homologous to DNA sequences present within the US2 gene coding region of the herpesvirus of turkeys genome. Preferably, this homology vector is designated 435-47.1.
The invention further provides a vaccine which comprises an effective immunizing amount of a recombinant herpesvirus of turkeys of the present invention and a suitable carrier.
Suitable carriers for the herpesvirus of turkeys are well known in the art and include proteins, sugars, etc. One example of such a suitable carrier is a physiologically balanced culture medium containing one or more stabilizing agents such as stabilized, hydrolyzed proteins, lactose, etc.
For purpose of this invention, an "effective immunizing amount" of recombinant herpesvirus of the present invention is within the range of 10.sup.2 to 10.sup.9 PFU/dose.
The present invention also provides a method of immunizing a fowl. For purposes of this invention, this includes immunizing a fowl against infectious bursal disease virus, Marek's disease virus, Newcastle disease virus, infectious laryngotracheitis virus, or infectious bronchitis virus. The method comprises administering to the fowl an effective immunizing dose of the vaccine of the present invention. The vaccine may be administered by any of the methods well known to those skilled in the art, for example, by intramuscular, subcutaneous, intraperitoneal or intravenous injection. Alternatively, the vaccine may be administered intranasally or orally.
The present invention also provides a host cell infected with a recombinant herpesvirus of turkeys. Preferably, the host cell is an avian cell.
For purposes of this invention, a "host cell" is a cell used to propagate a vector and its insert. Infecting the cell was accomplished by methods well known to those skilled in the art, for example, as set forth in DNA TRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUS in Materials and Methods.
A recombinant herpesvirus of turkeys of the present invention provide a way for distinguishing an animal vaccinated with the vaccine of the present invention from an animal infected with a naturally-occurring, wild-type infectious bursal disease virus, Marek's disease virus, Newcastle disease virus, infectious laryngotracheitis virus, or infectious bronchitis virus. This is possible because recombinant herpesvirus of turkeys contain foreign DNA which encodes a limited number of antigens from the above mentioned viruses that are needed to confer protective immunity to the corresponding pathogens. Consequently, host animals vaccinated with the recombinant herpesvirus of turkeys can be distinguished from those which have been infected with wild-type infectious bursal disease virus, Marek's disease virus, Newcastle disease virus, infectious laryngotracheitis virus, or infectious bronchitis virus by the absence of antigens that are normally present in the wild type viruses.
Methods for constructing, selecting and purifying recombinant herpesvirus of turkeys are detailed below in Materials and Methods.
MATERIALS AND METHODS
PREPARATION OF HERPESVIRUS OF TURKEYS STOCK SAMPLES. Herpesvirus of turkeys stock samples were prepared by infecting tissue culture cells at a multiplicity of infection of 0.01 PFU/cell in Dulbecco's Modified Eagle Medium (DMEM) containing 2 Mm glutamine, 100 units/ml penicillin, 100 units/ml streptomycin (these components are obtained from Irvine Scientific or an equivalent supplier, and hereafter are referred to as complete DME medium) plus 1% fetal bovine serum. After cytopathic effect was complete, the medium and cells were harvested and the cells were pelleted at 3000 rpm for 5 minutes in a clinical centrifuge. Infected cells were resuspended in complete medium containing 20% fetal bovine serum, 10% DMSO and stored frozen at -70.degree. C.
PREPARATION OF HERPESVIRUS OF TURKEY DNA. All manipulations of herpesvirus of turkey (HVT) were made using strain FC-126 (ATCC #584-C). For the preparation of HVT viral DNA from the cytoplasm of infected cells, primary chicken embryo fibroblasts were infected at a MOI sufficient to cause extensive cytopathic effect before the cells overgrew. All incubations were carried out at 39.degree. C. in a humidified incubator with 5% CO.sub.2 in air. Best DNA yields were obtained by harvesting monolayers which were maximally infected, but showing incomplete cell lysis (typically 5-7 days). Infected cells were harvested by scraping the cells into the medium using a cell scraper (Costar brand). The cell suspension was centrifuged at 3000 rpm for 10 minutes at 5.degree. C. in a GS-3 rotor (Sorvall Instruments). The resultant pellet was resuspended in cold PBS (20 ml/Roller Bottle) and subjected to another centrifugation for 10 minutes at 3000 rpm in the cold. After decanting the PBS, the cellular pellet was resuspended in 4 ml/roller bottle of RSB buffer (10 mM Tris pH 7.5, 1 mM EDTA, and 1.5 mM MgCl.sub.2). NP40 (Nonidet P-40.TM.; Sigma) was added to the sample to a final concentration of 0.5% and allowed to incubate on ice for 15 minutes with occasional mixing. The sample was centrifuged for 10 minutes at 3000 rpm in the cold to pellet the nuclei and remove cellular debris. The supernatant fluid was carefully transferred to a 15 ml Corex centrifuge tube. Both EDTA (0.5M pH 8.0) and SDS (sodium dodecyl sulfate; stock 20%) were added to the sample to final concentrations of 5 mM and 1%, respectively. One hundred .mu.l of proteinase-K (10 mg/ml; Boehringer Mannheim) was added per 4 ml of sample, mixed, and incubated at 45.degree. C. for 1-2 hours. After this period, an equal volume of water-saturated phenol was added to the sample and gently mixed by hand. The sample was spun in a clinical centrifuge for 5 minutes at 3000 rpm to separate the phases. NaAc was added to the aqueous phase to a final concentration of 0.3M (stock solution 3M pH 5.2), and the nucleic acid precipitated at -70.degree. C. for 30 minutes after the addition of 2.5 volumes of cold absolute ethanol. DNA in the sample was pelleted by spinning for 20 minutes to 8000 rpm in an HB-4 rotor at 5.degree. C. The supernatant was carefully removed and the DNA pellet washed once with 25 ml of 80% ethanol. The DNA pellet was dried briefly by vacuum (2-3 minutes), and resuspended in 50 .mu.l/roller bottle of infected cells of TE buffer (10 mM Tris pH 7.5, 1 mM EDTA). Typically, yields of viral DNA ranged between 5-10 .mu.g/roller bottle of infected cells. All viral DNA was stored at approximately 10.degree. C.
POLYMERASE FILL-IN REACTION. DNA was resuspended in buffer containing 50 mM Tris pH 7.4, 50 mM KCl, 5 mM MgCl.sub.2, and 400 micromolar each of the four deoxynucleotides. Ten units of Klenow DNA polymerase (BRL) were added and the reaction was allowed to proceed for 15 minutes at room temperature. The DNA was then phenol extracted and ethanol precipitated as above.
DNA SEQUENCING. Sequencing was performed using the USB Sequenase Kit and .sup.35 S-dATP (NEN). Reactions using both the dGTP mixes and the dITP mixes were performed to clarify areas of compression. Alternatively, compressed areas were resolved on formamide gels. Templates were double-stranded plasmid subclones or single stranded M13 subclones, and primers were either made to the vector just outside the insert to be sequenced, or to previously obtained sequence. Sequence obtained was assembled and compared using Dnastar software. Manipulation and comparison of sequences obtained was performed with Superclone and Supersee programs from Coral Software.
MOLECULAR BIOLOGICAL TECHNIQUES. Techniques for the manipulation of bacteria and DNA, including such procedures as digestion with restriction endonucleases, gel electrophoresis, extraction of DNA from gels, ligation, phosphorylation with kinase, treatment with phosphatase, growth of bacterial cultures, transformation of bacteria with DNA, and other molecular biological methods are described by Maniatis et al (1982) and Sambrook et al (1989). The polymerase chain reaction (PCR) was used to introduce restriction sites convenient for the manipulation of various DNAs. The procedures used are described by Innis et al (1990). In general amplified fragments were less than 500 base pairs in size and critical regions of amplified fragments were confirmed by DNA sequencing. Except as noted, these techniques were used with minor variation.
SOUTHERN BLOTTING OF DNA. The general procedure for Southern blotting was taken from Maniatis et al. (1982). DNA was blotted to nitrocellulose filters (S&S BA85) in 20.times. SSC (1.times.ssc=0.15M NaCl, 0.015M sodium citrate, pH 7.0), and prehybridized in hybridization solution consisting of 30% formamide, 1.times. Denhardt's solution (0.02% polyvinylpyrrolidone (PVP), 0.02% bovine serum albumin (BSA), 0.02% Ficoll), 6.times. SSC, 50 mM NaH.sub.2 PO.sub.4, pH 6.8, 200 .mu.g/ml salmon sperm DNA for 4-24 hours at 55.degree. C. Labeled probe DNA was added that had been labeled by nick translation using a kit from Bethesda Research Laboratories (BRL) and one .sup.32 P-labeled nucleotide. The probe DNA was separated from the unincorporated nucleotides by NACS column (BRL) or on a Sephadex G50 column (Pharmacia). After overnight hybridization at 55.degree. C., the filter was washed once with 2.times. SSC at room temperature followed by two washes with 0.1.times. SSC, 0.1% sodium dodecyl sulfate (SDS) for 30 minutes at 55.degree. C. The filter was dried and autoradiographed.
cDNA CLONING PROCEDURE. cDNA cloning refers to the methods used to convert RNA molecules into DNA molecules following state of the art procedures. Applicants' methods are described in (Gubler and Hoffman, 1983). Bethesda Research Laboratories (Gaithersburg, Md.) have designed a cDNA Cloning Kit that is very similar to the procedures used by applicants, and contains a set of reagents and protocols that may be used to duplicate our results.
For cloning virus mRNA species, a host cell line sensitive to infection by the virus was infected at 5-10 plaque forming units per cell. When cytopathic effect was evident, but before total destruction, the medium was removed and the cells were lysed in 10 mls lysis buffer (4 M guanidine thiocyanate, 0.1% antifoam A, 25 mM sodium citrate pH 7.0, 0.5% N-lauroyl sarcosine, 0.1 M beta-mercaptoethanol). The cell lysate was poured into a sterilized Dounce homogenizer and homogenized on ice 8-10 times until the solution was homogenous. For RNA purification, 8 mls of cell lysate were gently layered over 3.5 mls of CsCl solution (5.7 M CsCl, 25 mM sodium citrate pH 7.0) in a Beckman SW41 centrifuge tube. The samples were centrifuged for 18 hrs at 20.degree. C. at 36000 rpm in a Beckman SW41 rotor. The tubes were put on ice and the supernatants from the tubes were carefully removed by aspiration to leave the RNA pellet undisturbed. The pellet was resuspended in 400 .mu.l glass distilled water, and 2.6 mls of guanidine solution (7.5 M guanidine-HCl, 25 mM sodium citrate pH 7.0, 5 mM dithiothreitol) were added. Then 0.37 volumes of 1 M acetic acid were added, followed by 0.75 volumes of cold ethanol and the sample was put at -20.degree. C. for 18 hrs to precipitate RNA. The precipitate was collected by centrifugation in a Sorvall centrifuge for 10 min at 4.degree. C. at 10000 rpm in an SS34 rotor. The pellet was dissolved in 1.0 ml distilled water, recentrifuged at 13000 rpm, and the supernatant saved. RNA was re-extracted from the pellet 2 more times as above with 0.5 ml distilled water, and the supernatants were pooled. A 0.1 volume of 2 M potassium acetate solution was added to the sample followed by 2 volumes of cold ethanol and the sample was put at -20.degree. C. for 18 hrs. The precipitated RNA was collected by centrifugation in the SS34 rotor at 4.degree. C. for 10 min at 10000 rpm. The pellet was dissolved in 1 ml distilled water and the concentration taken by adsorption at A260/280. The RNA was stored at -70.degree. C.
mRNA containing polyadenylate tails (poly-A) was selected using oligo-dT cellulose (Pharmacia #27 5543-0). Three mg of total RNA was boiled and chilled and applied to a 100 mg oligo-dT cellulose column in binding buffer (0.1 M Tris pH 7.5, 0.5 M LiCl, 5 mM EDTA pH 8.0, 0.1% lithium dodecyl sulfate). The retained poly-A.sup.+ RNA was eluted from the column with elution buffer (5 mM Tris pH 7.5, 1 mM EDTA pH 8.0, 0.1% sodium dodecyl sulfate). This mRNA was reapplied to an oligo-dT column in binding buffer and eluted again in elution buffer. The sample was precipitated with 200 mM sodium acetate and 2 volumes cold ethanol at -20.degree. C. for 18 hrs. The RNA was resuspended in 50 .mu.l distilled water.
Ten .mu.g poly-A.sup.+ RNA was denatured in 20 mM methyl mercury hydroxide for 6 min at 22.degree. C. .beta.-mercaptoethanol was added to 75 mM and the sample was incubated for 5 min at 22.degree. C. The reaction mixture for first strand cDNA synthesis in 0.25 ml contained 1 .mu.g oligo-dT primer (P-L Bio-chemicals) or 1 .mu.g synthetic primer, 28 units placental ribonuclease inhibitor (Bethesda Research Labs #5518SA), 100 mM Tris pH 8.3, 140 mM KCl, 10 mM MgCl.sub.2, 0.8 mM DATP, dCTP, dGTP, and dTTP (Pharmacia), 100 microcuries .sup.32 P-labeled dCTP (New England Nuclear #NEG-013H), and 180 units AMV reverse transcriptase (Molecular Genetics Resources #MG 101). The reaction was incubated at 42.degree. C. for 90 min, and then was terminated with 20 mM EDTA pH 8.0. The sample was extracted with an equal volume of phenol/chloroform (1:1) and precipitated with 2 M ammonium acetate and 2 volumes of cold ethanol -20.degree. C. for 3 hrs. After precipitation and centrifugation, the pellet was dissolved in 100 .mu.l distilled water. The sample was loaded onto a 15 ml G-100 Sephadex column (Pharmacia) in buffer (100 mM Tris pH 7.5, 1 mM EDTA pH 8.0, 100 mM NaCl). The leading edge of the eluted DNA fractions was pooled, and DNA was concentrated by lyophilization until the volume was about 100 .mu.l, then the DNA was precipitated with ammonium acetate plus ethanol as above.
The entire first strand sample was used for second strand reaction which followed the Gubler and Hoffman (1983) method except that 50 .mu.g/ml dNTP's, 5.4 units DNA polymerase I (Boerhinger Mannheim #642-711), and 100 units/ml E. coli DNA ligase (New England Biolabs #205) in a total volume of 50 microliters were used. After second strand synthesis, the CDNA was phenol/chloroform extracted and precipitated. The DNA was resuspended in 10 .mu.l distilled water, treated with 1 .mu.g RNase A for 10 min at 22.degree. C., and electrophoresed through a 1% agarose gel (Sigma Type II agarose) in 40 mM Tris-acetate buffer pH 6.85. The gel was stained with ethidium bromide, and DNA in the expected size range was excised from the gel and electroeluted in 8 mM Tris-acetate pH 6.85. Electroeluted DNA was lyophilized to about 100 microliters, and precipitated with ammonium acetate and ethanol as above. The DNA was resuspended in 20 .mu.l water.
Oligo-dC tails were added to the DNA to facilitate cloning. The reaction contained the DNA, 100 mM potassium cacodylate pH 7.2, 0.2 mM dithiothreitol, 2 mM CaCl.sub.2, 80 .mu.moles dCTP, and 25 units terminal deoxynucleotidyl transferase (Molecular Genetic Resources #S1001) in 50 .mu.l. After 30 min at 37.degree. C., the reaction was terminated with 10 mM EDTA, and the sample was phenol/chloroform extracted and precipitated as above.
The dC-tailed DNA sample was annealed to 200 ng plasmid vector pBR322 that contained oligo-dG tails (Bethesda Research Labs #5355 SA/SB) in 200 .mu.l of 0.01 M Tris pH 7.5, 0.1 M NaCl, 1 mM EDTA pH 8.0 at 65.degree. C. for 2 min and then 57.degree. C. for 2 hrs. Fresh competent E. coli DH-1 cells were prepared and transformed as described by Hanahan (1983) using half the annealed cDNA sample in twenty 200 .mu.l aliquots of cells. Transformed cells were plated on L-broth agar plates plus 10 .mu.g/ml tetracycline. Colonies were screened for the presence of inserts into the ampicillin gene using Ampscreen.backslash.(Bethesda Research Labs #5537 UA), and the positive colonies were picked for analysis.
DNA TRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUS. The method is based upon the polybrene-DMSO procedure of Kawai and Nishizawa (1984) with the following modifications. Generation of recombinant HVT virus is dependent upon homologous recombination between HVT viral DNA and the plasmid homology vector containing the desired foreign DNA flanked by the appropriate herpesvirus cloned sequences. Transfections were carried out in 6 cm plates (Corning plastic) of 50% confluent primary chick embryo fibroblast (CEF) cells. The cells were plated out the day before in CEF growth media (1.times. F10/199, 5% fetal calf serum, 2% glutamine, 1% non-essential amino acids, and 2% penicillin/streptomycin) containing 4 .mu.g/ml polybrene (stock 4 mg/ml in 1.times.HBSS). For cotransfections into CEF cells, 5 .mu.g of the plasmid homology vector was mixed with 5 .mu.g of intact HVT DNA, and suspended in 1 ml of CEF media containing 30 .mu.g/ml polybrene (stock 4 mg/ml in 1.times. HBSS). The DNA-polybrene suspension (1 ml) was then added to a 6 cm plate of CEF cells from which the media had been aspirated, and incubated at 39.degree. C. for 30 minutes. The plates were rocked periodically during this time to redistribute the inoculum. After this period, 4 ml of CEF growth media was added directly to wash plate, and incubated an additional 2.5 hours at 39.degree. C. At this time, the media was removed from each plate, and the cells shocked with 2 ml of 30% DMSO (Dimethyl Sulfoxide, J.T. Baker Chemical Co.) in 1.times. HBSS for 4 minutes at room temperature. The 30% DMSO was carefully removed and the monolayers washed once with 1.times. HBSS at room temperature. The cells were then incubated at 39.degree. C. after the addition of 5 mls of CEF growth media. The next day, the media was changed to remove any last traces of DMSO and to stimulate cell growth. Cytopathic effect from the virus becomes apparent within 6 days. Generation of a high titer stock (80%-90% CPE) can usually be made within 1 week from this date. HVT stock samples were prepared by resuspending the infected cells in CEF growth media containing 20% fetal calf serum, 10% DMSO and stored at -70.degree. C.
PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The ability to generate herpesviruses by cotransfection of cloned overlapping subgenmoic fragments has been demonstrated for pseudorabies virus (Zijl et al., 1988). If deletions and/or insertions are engineered directly into the subgenomic fragments prior to the cotransfection, this procedure results in a high frequency of viruses containing the genomic alteration, greatly reducing the amount of screening required to purify the recombinant virus. We have used this procedure to construct recombinant HVT.
A library of subclones containing overlapping HVT subgenomic fragments was generated as follows. HVT DNA was obtained from the American Type Culture Collection (FC-126 ("Calnek")). It was sheared and then size selected on a glycerol gradient as described by van Zijl et al.,(1988) with 40-50 kb fragments chosen as the insert population. The pooled fractions were diluted twofold with TE, one-tenth volume of 3M NaAc and 2.5 volumes of ethanol were added, and the DNA was precipitated at 30K rpm in a Beckman SW41 rotor for 1 hr. The sheared fragments were given blunt ends by initial treatment with T4 DNA polymerase, using low DNTP concentrations to promote 3' overhang removal, followed by treatment with Klenow polymerase to fill in recessed 3' ends. These insert fragments were then ligated to a pWE15 (Strategene) cosmid vector, which had been digested with BamHI, treated with calf intestinal phosphatase, and made blunt by treatment with Klenow polymerase. The ligated mixture was then packaged using Gigapack XL packaging extracts (Stratagene). Ligation and packaging was as recommended by the manufacturer.
Published restriction maps for the enzymes BamHI, HindIII, and XhoI permitted the use of subcloned fragments as specific probes to screen the cosmid library for subclones spanning the genome. Probes were generated from subcloned restriction fragments. The fragments were then labeled using a non-radioactive system (Genius, Boehringer Mannheim). Screening was facilitated by picking colonies to media followed by growth overnight. Sets of five filters and a master plate were stamped from microtiter dish and again grown overnight. Glycerol was added to the wells to 15% and the plates were frozen at -20.degree. C. to provide stock cultures of each colony. Filters were BioRad Colony Lift Membranes and were treated and hybridized per manufacturer's instructions, and washed in 0.1.times. SSC, 0.1% SDS, 65.degree. C. Clones which hybridized with the non-radioactive probe were detected according to the Genius kit directions.
Colonies were selected for further analysis on the basis of their hybridization to two or more of the specific probes. These were then digested with BamHI, and compared to published maps of HVT (Buckmaster et al., 1988). The three cosmids (407-32.2C3,407-32.1G7, and 407-32.5G6) were obtained in this manner. A detailed description of each clone is given below. It was found that chloramphenicol amplification (Maniatis et al.,1982) was necessary to achieve reasonable yields of DNA from these clones. In addition, one cosmid clone (407-32.5G6) was unstable and had to be grown from the original frozen stock in order to obtain satisfactory DNA preparations.
The pWE15 vector allows the inserts to be excised with NotI. However, four NotI sites are present in the HVT genome, so that inserts spanning these sites cannot be excised with NotI. Two of the NotI sites are present in the BamHI #2 fragment of HVT, this fragment was cloned directly in pSP64. The other two sites are present in the unique short region within the BamHI #1 fragment. This fragment was cloned directly in the pWE15 vector. The three sheared cosmids and the two BamHI fragments cover all but a small portion of the ends of the HVT genome. Because these regions are repeated in the internal portions of the genome, all of the genetic information is available.
A StuI site within the HVT US2 gene was established as a useful site for foreign DNA insertion utilizing the HOMOLOGOUS RECOMBINATION PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUSES (see Example 6). The HVT US2 gene is located within the BamHI #1 fragment which contains five Stul sites. To facilitate the use of this site for insertion of foreign DNA by the StuI site within the US2 gene was converted to a unique HindIII site. This was accomplished by partially digesting the BamHI #1 subclone with StuI, and then inserting a 10 kb fragment conferring kanomycin resistance (Neo.sup.R) into the site using HindIII linkers. The kanomycin resistance gene allowed positive selection of recombinant clones. The Neo.sup.R fragment was removed by digestion with HindIII followed by religation generating clone 430-84.215.
DNA was prepared for reconstruction experiments by restriction digestion with enzymes which cut the subclones outside or flanking the HVT insertions. In some instances, one cosmid in a reconstruction was used undigested. Digested DNAs were extracted once with phenol and precipitated with ethanol. DNA was resuspended at a concentration of 0.5 to 1 ug/ml. Viral reconstruction experiments were performed using Lipofectin (BRL) to mediate transfection. Two to three micrograms each of subclone were added to 0.5 ml of MEM media (Earle's salts) supplemented with 1% non-essential amino acids and 2% penicillin/Streptomysin (MEM+). Controls consisted of MEM+ with no DNA, with several ug of HVT DNA, or with 4 out of 5 of the subclones. Separately, 30 .mu.l of the Lipofectin were added to another 0.5 ml. of MEM+. These two mixtures were then combined and incubated at RT for 15 minutes.
Chick embryo fibroblast (CEF) cells were prepared for transfection in the following manner. CEFs (Spafas) were grown in 6 well dishes at 39.degree. C. in F10/M199 (1:1) media containing 1% non-essential amino acids, 2% penicillin/streptomycin, and 5% fetal calf serum (CEF+). Cells were transfected at a confluence of 90-95%. For transfection, wells were aspirated and rinsed 3 times with MEM+, and then incubated 4 hours at 39.degree. C. with the 1 ml lipofectin/DNA mixture above. One ml more of CEF+ was then added to the wells, and cells were incubated overnight and fed with CEF+. Plates were then examined daily for the appearance of plaques.
Lipofectin with control HVT DNA resulted in the appearance of plaques within 5 days. When only four of the five subclones were used, no plaques were obtained. When the five overlapping genomic fragments of HVT were used to reconstruct the virus, plaques appeared anywhere from 5 to 19 days after the initial lipofection. In the case of plaques appearing late, plaques were not initially seen on the infected monolayer, and it was only after passaging the monolayer and replating on a larger surface that plaques appeared. After passaging, plaques generally appeared within 3 days. Recombinant viruses were plaque purified approximately three and then analyzed for insertion of foreign DNAs.
BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS. When the foreign gene encoded the enzyme .beta.-galactosidase, the plaques that contained the gene were visualized more easily. The chemical Bluogal.TM. (Bethesda Research Labs) was incorporated at the level of 200-300 .mu.g/ml into the agarose overlay during the plaque assay, and the plaques that expressed active .beta.-galactosidase turned blue. The blue plaques were then picked and purified by further blue plaque isolations. Other foreign genes were inserted by homologous recombination such that they replaced the .beta.-galactosidase gene; in this instance non-blue plaques were picked for purification of the recombinant virus.
SCREEN FOR FOREIGN GENE EXPRESSION IN RECOMBINANT HVT USING BLACK PLAQUE ASSAYS. To analyze expression of foreign antigens expressed by recombinant HVT viruses, monolayers of CEF cells are infected with recombinant HVT, overlaid with nutrient agarose media and incubated for 4-5 days at 39.degree. C. Once plaques have developed, the agarose overlay is removed from the dish, the monolayer rinsed 1.times. with PBS, fixed with 100% methanol for 10 minutes at room temperature and the cells air dried. After re-hydrating the plate with PBS, the primary antibody is diluted to the appropriate dilution with PBS and incubated with the cell monolayer for 2 hours to overnight at room temperature. Unbound antibody is then removed from the cells by washing three times with PBS at room temperature. An alkaline phosphatase conjugated secondary antibody is diluted with PBS and incubated with the cells for 2 hours at room temperature. Unbound secondary antibody is then removed by washing the cells three times with PBS at room temperature. Next, the monolayer is rinsed in color development buffer (100 mM Tris pH 9.5/100 mM NaCl/5 mM MgCl2), and then incubated 10 minutes to overnight at room temperature with freshly prepared substrate solution (0.3 mg/ml Nitro Blue tetrazolium+0.15 mg/ml 5-Bromo-4-Chloro-3-Indolyl Phosphatase in color development buffer.) Finally, the reaction is stopped by replacing the substrate solution with TE (10 mM Tris, pH7.5/1 mM EDTA). Plaques expressing the correct antigen will stain black.
PLAQUE HYBRIDIZATION PROCEDURE FOR ASSESSING THE PURITY OF RECOMBINANT HVT STOCKS. When no suitable immunological reagent exists to detect the presence of a particular antigen in a recombinant HVT virus, plaque hybridization can be used to assess the purity of a stock. Initially, CEF cell monolayers are infected with various dilutions of the viral stocks to give .noteq.50-100 plaques/10 cm.dish, overlaid with nutrient agarose media and incubated for 4-5 days at 39.degree. C. Once plaque development occurs, the position of each plaque is marked on bottom of the dish. The agarose overlay is then removed, the plate washed with PBS, and the remaining CEF monolayer is transferred to a NC membrane or BioRad nylon membrane pre-wetted with PBS (making note of the membrane position relative to the dish). Cells contained on the NC membranes are then lysed by placing the membranes in 1.5 mls of 1.5M NaCl and 0.5M NaOH for five minutes. The membranes are neutralized by placing them in 1.5 mls of 3M Sodium acetate (pH 5.2) for five minutes. DNA from the lysed cells is then bound to the NC membranes by baking at 80.degree. C. for one hour. After this period the membranes are prehybridized in a solution containing 6.times. SSC, 3% skim milk, 0.5% SDS, (.+-.) salmon sperm DNA (50 .mu.g/ml) for one hour at 65.degree. C. Radio-labeled probe DNA (alpha 32P-dCTP) is then added and the membranes incubated at 65.degree. C. overnight (.noteq.12 hours). After hybridization the NC membranes are washed two times (30 minutes each) with 2.times. SSC at 65.degree. C., followed by two additional washes at 65.degree. C. with 0.5.times. SSC. The NC membranes are then dried and exposed to X-ray film (Kodak X-OMAT,AR) at -70.degree. C. for 12 hours. Positive signals are then aligned with the position of the plaques on the dish and purity of the stock is recorded as the percentage of positive plaques over the total.
CONSTRUCTION OF HOMOLOGY VECTOR FOR INSERTION OF THE BETA-GALACTOSIDASE GENE INTO HVT US2 GENE. The beta-galactosidase (lacz) gene was inserted into the HVT EcoRI # 7 fragment at the unique StuI site. The marker gene is oriented in the same direction as the US2 gene. A detailed description of the marker gene is given in FIG. 7. It is constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources with the synthetic DNA sequences indicated in FIG. 7. Fragment 1 is an approximately 413 base pair SalI to BamHI restriction sub-fragment of the PRV BamHI restriction fragment 10 (Lomniczi et al., 1984). Fragment 2 is an approximately 3010 base pair BamHI to PvuII restriction fragment of plasmid pJF751 (Ferrari et al., 1985). Fragment 3 is an approximately 754 base pair NdeI to SalI restriction sub-fragment of the PRV BamHI restriction fragment #7 (Lomniczi et al., 1984).
SUBGENOMIC CLONE 172-07.BA2. Plasmid 172-07.BA2 was constructed for the purpose of generating recombinant HVT. It contains an approximately 25,000 base pair region of genomic HVT DNA. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining two restriction fragments from the following sources. The first fragment is an approximately 2999 base pair BamHI to BamHI restriction fragment of pSP64 (Promega). The second fragment is the approximately 25,000 base pair BamHI #2 fragment of HVT (Buckmaster et al., 1988).
HOMOLOGY VECTOR 172-29.31. The plasmid 172-29.31 was constructed for the purpose of inserting foreign DNA into HVT. It contains a unique XhoI restriction enzyme site into which foreign DNA may be inserted. When a plasmid containing a foreign DNA insert at the XhoI site is used according to the DNA COTRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUSES or the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS a virus containing the foreign DNA will result. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining two restriction fragments from the following sources. The first fragment is an approximately 2999 base pair BamHI to BamHI restriction fragment of pSP64 (Promega). The second fragment is the approximately 3300 base pair BamHI #16 fragment of HVT (Buckmaster et al., 1988). The complete sequence of the BamHI #16 fragment is given in SEQ ID NO:3. Note that the fragment was cloned such that the UL43 ORF is in the opposite transcriptional orientation to the pSP64 .beta.-lacatamase gene.
HOMOLOGY VECTOR 172-63.1. The plasmid 172-63.1 was constructed for the purpose of inserting foreign DNA into HVT. It contains a unique XhoI restriction enzyme site into which foreign DNA may be inserted. When a plasmid containing a foreign DNA insert at the XhoI site is used according to the DNA COTRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUSES or the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS a virus containing the foreign DNA will result. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining two restriction fragments from the following sources. The first fragment is an approximately 2999 base pair EcoRI to EcoRI restriction fragment of pSP64 (Promega). The second fragment is the approximately 5500 base pair EcoRI #9 fragment of HVT. Note that the EcoRI fragment was cloned such that the unique XhoI site is closest to the unique HindIII site in the pSP64 vector.
HOMOLOGY VECTORS 255-18.B16. The plasmid 255-18.B16 was constructed for the purpose of inserting the NDV HN and F genes into HVT. The NDV HN and F genes were inserted as a SalI fragment into the homology vector 172-29.31 at the XhoI site. The NDV HN and F genes were inserted in the same transcriptional orientation the UL43 ORF in the parental homology vector. A detailed description of the SalI fragment is shown in FIG. 12. The inserted SalI fragment may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources with the synthetic DNA sequences indicated in FIG. 12. Fragment 1 is an approximately 416 base pair SalI to BamHI restriction sub-fragment of the PRV BamHI restriction fragment 10 (Lomniczi et al., 1984). Fragment 2 is an approximately 3009 base pair BamHI to PvuII fragment of the plasmid pJF751 (Ferrari et al., 1985). Fragment 3 is an approximately 1200 base pair AvaII to EcoRI restriction fragment of full length NDV HN cDNA. Fragment 4 is an approximately 179 base pair EcoRI to PvuII restriction fragment of the plasmid pSP64 (Promega). Fragment 5 is an approximately 357 base pair SmaI to BamHI restriction sub-fragment of the HSV-l BamHI restriction fragment N. Fragment 6 is an approximately 1812 base pair BamHI to PstI restriction fragment of the full length NDV F cDNA. Fragment 7 is an approximately 235 base pair PstI to ScaI restriction fragment of the plasmid pBR322.
SUBGEMOMIC CLONE 378-50.BA1. Cosmid 378-50.BA1 was constructed for the purpose of generating recombinant HVT. It contains an approximately 29,500 base pair region of genomic HVT DNA. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This cosmid may be constructed by joining two restriction fragments from the following sources. The first fragment is an approximately 8164 base pair BamHI to BamHI restriction fragment of pWE15 (Stratagene). The second fragment is the approximately 29,500 base pair BamHI #1 fragment of HVT (Buckmaster et al., 1988).
SUBGEMOMIC CLONE 407-32.1C1. Cosmid 407-32.1C1 was constructed for the purpose of generating recombinant HVT. It contains an approximately 38,850 base pair region of genomic HVT DNA (see FIG. 8). This region includes BamHI fragments 11, 7, 8, 21, 6, 18, approximately 1250 base pairs of fragment 13, and approximately 6,700 base pairs of fragment 1. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This cosmid maybe constructed as described above in the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS. It was isolated from the sheared DNA library by screening with the probes P1 and P4 (described in FIG. 8). A bacterial strain containing this cosmid has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
SUBGEMOMIC CLONE 407-32.2C3. Cosmid 407-32.2C3 was constructed for the purpose of generating recombinant HVT. It contains an approximately 40,170 base pair region of genomic HVT DNA (see FIG. 8). This region includes BamHI fragments 10, 14, 19, 17, 5, and approximately 2,100 base pairs of fragment 2. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This cosmid may be constructed as described above in the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS. It was isolated from the sheared DNA library by screening with the probes P1 and P2 (described in FIG. 8). A bacterial strain containing this cosmid has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
SUBGEMOMIC CLONE 407-32.5G6. Cosmid 407-32.5G6 was constructed for the purpose of generating recombinant HVT. It contains an approximately 40,000 base pair region of genomic HVT DNA (see FIG. 8). This region includes BamHI fragments 9, 3, 20, 12, 16, 13, approximately 1,650 base pairs of fragment 2, and approximately 4,000 base pairs of fragment 11. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This cosmid may be constructed as described above in the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS. It was isolated from the sheared DNA library by screening with the probes P2 and P3 (described in FIG. 8). A bacterial strain containing this cosmid has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
HOMOLOGY VECTOR 435-47.1. The plasmid 435-47.1 was constructed for the purpose of inserting foreign DNA into HVT. It contains a unique HindIII restriction enzyme site into which foreign DNA may be inserted. When a plasmid containing a foreign DNA insert at the HindIII site is used according to the DNA COTRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUSES or the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS a virus containing the foreign DNA will result. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining two restriction fragments from the following sources. The first fragment is an approximately 2999 base pair EcoRI to EcoRI restriction fragment of pSP64 (Promega). The second fragment is the approximately 7300 base pair EcoRI #7 fragment of HVT. Note that the HindIII site of the pSP64 vector was removed by digesting the subclone with HindIII followed by a Klenow fill in reaction and religation. A synthetic HindIII linker (CAAGCTTG) was then inserted into the unique StuI site of the EcoRI #7 fragment.
SUBGEMOMIC CLONE 437-26.24. Plasmid 437-26.24 was constructed for the purpose of generating recombinant HVT. It contains an approximately 13,600 base pair region of genomic HVT DNA. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining two restriction fragments from the following sources. The first fragment is an approximately 2970 base pair HindIII to BamHI restriction fragment of pSP64 (Promega). The second fragment is the approximately 13,600 base pair BamHI to StuI sub-fragment of the BamHI #2 fragment of HVT (Buckmaster et al., 1988). Note that the BamHI #2 fragment contains five StuI sites, the site utilized in this subcloning was converted to a HindIII site as described in the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS.
SUBGEMOMIC CLONE 437-26.26. Plasmid 437-26.26 was constructed for the purpose of generating recombinant HVT. It contains an approximately 15,300 base pair region of genomic HVT DNA. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining two restriction fragments from the following sources. The first fragment is an approximately 2970 base pair HindIII to BamHI restriction fragment of pSP64 (Promega). The second fragment is the approximately 15,300 base pair BamHI to StuI sub-fragment of the BamHI #2 fragment of HVT (Buckmaster et al., 1988). Note that the BamHI #2 fragment contains five StuI sites, the site utilized in this subcloning was converted to a HindIII site as described in the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS.
HOMOLOGY VECTORS 456-18.18 and 456-17.22. The plasmids 456-18.18 and 456-17.22 were constructed for the purpose of inserting the MDV gA and gB genes into HVT. The MDV genes were inserted as a cassette into the homology vector 435-47.1 at the unique HindIII site. The MDV genes were inserted at the blunt ended HindIII site as a blunt ended PstI to EcoRI fragment (see FIG. 10). The HindIII and EcoRI sites were blunted by the Klenow fill in reaction. The PstI site was blunted by the T4 DNA polymerase reaction. Note that the MDV cassette was inserted in both orientations. Plasmid 456-18.18 contains the MDV genes inserted in the opposite transcriptional orientation to the US2 gene in the parental homology vector. Plasmid 456-17.22 contains the MDV genes inserted in the same transcriptional orientation as the US2 gene in the parental homology vector. A detailed description of the MDV cassette is given in FIG. 10. It may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources with the synthetic DNA sequences indicated in FIG. 10. Fragment 1 is an approximately 2178 base pair PvuII to EcoRV restriction sub-fragment of the MDV EcoRI 6.9 KB genomic restriction fragment (Ihara et al., 1989). Fragment 2 is an approximately 3898 base pair SalI to EcoRI genomic MDV fragment (Ross, et al., 1989).
HOMOLOGY VECTOR 528-03.37. The plasmid 528-03.37 was constructed for the purpose of inserting the infectious laryngotracheitis (ILT) virus gD gene into HVT. The gD gene followed by the PRV gX poly adenylation signal was inserted as a cassette into the homology vector 435-47.1 at the unique HindIII site. The cassette may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources. The first fragment is an approximately 2060 base pair EcoRI to BclI restriction sub-fragment of the ILT KpnI genomic restriction fragment #8 (10.6 KB). The second fragment is an approximately 754 base pair NdeI to SalI restriction sub-fragment of the PRV BamHI restriction fragment #7 (Lomniczi et al., 1984). Note that the fragments are oriented such that BclI and NdeI sites are contiguous.
HOMOLOGY VECTOR 528-11.43. The plasmid 528-11.43 was constructed for the purpose of inserting the infectious laryngotracheitis (ILT) virus gB gene (A. M. Grifin, 1991) into HVT. The gB gene was inserted as an EcoRI fragment into the homology vector 435-47.1 at the unique HindIII site. The gB gene was inserted at the blunt ended HindIII site as a blunt ended EcoRI fragment. The HindIII and EcoRI sites were blunted by the Klenow fill in reaction. The gB gene was inserted in the same transcriptional orientation as the US2 gene in the parental homology vector. The EcoRI fragment may be obtained as a 3.0 KB ILT virus genomic fragment.
HOMOLOGY VECTOR 518-46.B3. The plasmid 518-46.B3 was constructed for the purpose of inserting foreign DNA into HVT. It contains a unique HindIII restriction enzyme site into which foreign DNA may be inserted. When a plasmid containing a foreign DNA insert at the HindIII site is used according to the DNA COTRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUSES or the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS a virus containing the foreign DNA will result. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining three restriction fragments from the following sources. The first fragment is an approximately 1649 base pair PvuI to SalI restriction fragment of pSP64 (Promega). The second fragment is an approximately 1368 base pair PvuI to SalI restriction fragment of pSP65 (Promega). The third fragment is the approximately 3400 base pair XhoI to XhoI fragment of plasmid 437-47.1.
HOMOLOGY VECTOR 535-70.3. The plasmid 535-70.3 was constructed for the purpose of inserting the MDV gB, and gA genes and the NDV F gene into HVT. The F gene was inserted as a cassette into homology vector 456-17.22 at the HindIII site located between the MDV gA and gB genes (see FIG. 10A junction B). The F gene is under the control of the HCMV immediate early promoter and followed by the HSV-1 TK poly adenylation signal. The F gene was inserted in the same transcriptional orientation as the US2 gene in the parental homology vector. The cassette may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources. The first fragment is an approximately 1191 base pair PstI to AvaII restriction sub-fragment of the HCMV genomic XbaI E fragment (D. R. Thomsen, et al., 1981). The second fragment is an approximately 1812 base pair BamHI to PstI restriction fragment of the full length NDV F cDNA clone (B1 strain). The last fragment is an approximately 784 base pair SmaI to SmaI restriction sub-fragment of the HSV-1 BamHI restriction fragment Q (McGeoch, et al., 1985).
HOMOLOGY VECTOR 549-24.15. The plasmid 549-24.15 was constructed for the purpose of inserting the MDV gB, and gA genes and the NDV HN and F genes into HVT. The HN and F genes were inserted as a cassette into homology vector 456-17.22 at the HindIII site located between the MDV gA and gB genes (see FIG. 10A junction B). The HN and F genes are under the control of the PRV gpX and HCMV immediate early promoters respectively. The HN and F genes are followed by the PRV gX poly and HSV-1 TK adenylation signals respectively. The cassette may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources. The first fragment is an approximately 413 base pair SalI to BamHI restriction sub-fragment of the PRV BamHI fragment #10 (Lomniczi, et al., 1984). The second fragment is an approximately 1811 base pair AvaII to NaeI restriction fragment of the full length NDV HN cDNA clone (B1 strain). The third fragment is an approximately 754 base pair NdeI to SalI restriction sub-fragment of the PRV BamHI restriction fragment #7 (Lomniczi, et al., 1984). The fourth fragment is an approximately 1191 base pair PstI to AvaII restriction sub-fragment of the HCMV genomic XbaI E fragment (D. R. Thomsen, et al., 1981). The fifth fragment is an approximately 1812 base pair BamHI to PstI restriction fragment of the full length NDV F cDNA clone (B1 strain). The last fragment is an approximately 784 base pair SmaI to SmaI restriction sub-fragment of the HSV-1 BamHI restriction fragment Q (McGeoch, et al., 1985).
HOMOLOGY VECTOR 549-62.10. The plasmid 549-62.10 was constructed for the purpose of inserting the MDV gB, and gA genes and the NDV HN gene into HVT. The HN gene was inserted as a cassette into homology vector 456-17.22 at the HindIII site located between the MDV gA and gB genes (see FIG. 10A junction B). The HN gene is under the control of the PRV gpX promoter and followed by the PRV gX poly adenylation signal. The HN gene was inserted in the same transcriptional orientation as the US2 gene in the parental homology vector. The cassette may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources. The first fragment is an approximately 413 base pair SalI to BamHI restriction sub-fragment of the PRV BamHI fragment #10 (Lomniczi, et al., 1984). The second fragment is an approximately 1811 base pair AvaII to NaeI restriction fragment of the full length NDV HN CDNA clone (B1 strain). The last fragment is an approximately 754 base pair NdeI to SalI restriction sub-fragment of the PRV BamHI restriction fragment #7 (Lomniczi, et al., 1984).
SUBGENOMIC CLONE 550-60.6 Plasmid 550-60.6 was constructed for the purpose of generating recombinant HVT. It contains an approximately 12,300 base pair region of genomic HVT DNA. It may be used in conjunction with other subgenomic clones according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM OVERLAPPING SUBGENOMIC FRAGMENTS for the construction of recombinant HVT. This plasmid may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining two restriction fragments from the following sources. The first fragment is an approximately 4176 base pair EcoRV to BamHI restriction fragment of pBR322. The second fragment is the approximately 12,300 base pair sub-fragment of the BamHI #2 fragment of HVT (Buckmaster et al., 1988). This fragment was generated in the following manner. Plasmid 437-26.26 was linearized with HindIII and then resected with the ExoIII Mung Bean Deletion Kit (Stratagene). Samples from the 3 and 4 minute reactions were combined and digested with BamHI resulting in a population of fragments containing the desired 12,300 base pair sub-fragment. This population was cloned into the pBR322 fragment and the resulting clones were screened for the appropriate size and restriction map. Fortuitously the resected sub-fragment that generated clone 550-60.6 ended in the nucleotides GG which generated a second BamHI site when ligated to the EcoRV site (ATCC) of pBR322. A bacterial strain containing this plasmid has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
HOMOLOGY VECTORS 566-41.5. The plasmid 566-41.5 was constructed for the purpose of inserting the MDV gA, gB and gD genes into HVT. The MDV gD gene was inserted as a HindIII fragment into the homology vector 456-17.22 at the HindIII site located between MDV gA and gB (see FIG. 10). The MDV gene was inserted in the same transcriptional orientation as gA and gB in the parental homology vector. A detailed description of the HindIII fragment containing the MDV gD gene is shown in FIG. 11. Note that a herpesvirus polyadenation signal was added to the gD gene cassette. The inserted HindIII fragment may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources with the synthetic DNA sequences indicated in FIG. 11. Fragment 1 is an approximately 784 base pair SmaI to SmaI restriction sub-fragment of the HSV-1 BamHI restriction fragment Q (McGeoch et al., 1988). Note that this fragment is oriented such that the polyadenylation sequence (AATAAA) is located closest to junction B. Fragment 2 is an approximately 2177 base pair SalI to NcoI sub-fragment of the MDV BglII 4.2 KB genomic restriction fragment (Ross, et al., 1991).
HOMOLOGY VECTOR 567-72.1D. The plasmid 567-72.1D was constructed for the purpose of inserting the MDV gB, gA, and gD genes and the infectious bronchitis virus (IBV) matrix and spike genes into HVT. The IBV genes were inserted as a cassette into homology vector 566-41.5 at the unique NotI site located upstream of the MDV gD gene (see FIG. 11B junction C). The IBV spike and matrix genes are under the control of the HCMV immediate early and PRV gpX promoters respectively. The IBV spike and matrix genes are followed by the HSV-1 TK and PRV gX poly adenylation signals respectively. The IBV genes were inserted in the same transcriptional orientation as the US2 gene in the parental homology vector. The cassette may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources. The first fragment is an approximately 413 base pair SalI to BamHI restriction sub-fragment of the PRV BamHI fragment #10 (Lomniczi, et al., 1984). The second fragment contains amino acids 1 to 223 of the IBV matrix gene. The coding region was obtained from a cDNA clone of the Arkansas strain of IBV. The third fragment is an approximately 754 base pair NdeI to SalI restriction sub-fragment of the PRV BamHI restriction fragment #7 (Lomniczi, et al., 1984). The fourth fragment is an approximately 1191 base pair PstI to AvaII restriction sub-fragment of the HCMV genomic XbaI E fragment (D. R. Thomsen, et al., 1981). The fifth fragment contains amino acids 4 to 1162 of the IBV spike gene. The coding region was obtained from a cDNA clone of the Arkansas strain of IBV. The last fragment is an approximately 784 base pair SmaI to SmaI restriction sub-fragment of the HSV-1 BamHI restriction fragment Q (McGeoch, et al., 1985).
HOMOLOGY VECTOR 603-57.F1. The plasmid 603-57.F1 was constructed for the purpose of inserting the IBDV VP2 gene into HVT. The IBDV VP2 gene was inserted as a cassette into homology vector 435-47.1 at the unique HindIII site. The VP2 gene is under the control of the HCMV immediate early promoter and is followed by the HSV-1 TK poly adenylation signal. The VP2 gene was inserted in the same transcriptional orientation as the US2 in the parental homology vector. The cassette may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources. The first fragment is an approximately 1191 base pair PstI to AvaII restriction sub-fragment of the HCMV genomic XbaI E fragment (D. R. Thomsen, et al., 1981). The second fragment is an approximately 1081 base pair BclI to BamHI restriction sub-fragment of the full length IBDV cDNA clone (see SEQ ID NO:1). Note that the BclI site was introduced into the cDNA clone directly upstream of the VP2 initiator methionine by converting the sequence CGCAGC to TGATCA. The first and second fragments are oriented such that AvaII and BclI sites are contiguous. The third fragment is an approximately 784 base pair SmaI to SmaI restriction sub-fragment of the HSV-1 BamHI restriction fragment Q (McGeoch, et al., 1985).
HOMOLOGY VECTOR 633-13.27. The plasmid 633-13.27 was constructed for the purpose of inserting the MDV gB, gA and gD genes and the NDV HN and F genes into HVT. The HN and F genes are under the control of the PRV gpX and HCMV immediate early promoters respectively. The HN and F genes are followed by the PRV gX poly and HSV-1 TK adenylation signals respectively. All five genes were inserted in the same transcriptional orientation as the US2 gene in the parental homology vector. The genes were inserted in the following order MDV gA, NDV HN, NDV F, MDV gD, and MDV gB.
HOMOLOGY VECTOR 634-29.16. The plasmid 634-29.16 was constructed for the purpose of inserting the ILT virus gB and gD genes into HVT. The lacZ marker gene followed by the ILT gB and gD genes inserted as a cassette into the homology vector 172-29.31 at the unique XhoI site. The cassette may be constructed utilizing standard recombinant DNA techniques (Maniatis et al, 1982 and Sambrook et al, 1989), by joining restriction fragments from the following sources. The first fragment is an approximately 4229 base pair SalI to SalI restriction fragment derived from the lacZ marker gene described above and shown in FIG. 7. The second fragment is an approximately 2060 base pair EcoRI to BclI restriction sub-fragment of the ILT KpnI genomic restriction fragment #8 (10.6 KB). The third fragment is an approximately 754 base pair NdeI to SalI restriction sub-fragment of the PRV BamHI restriction fragment #7 (Lomniczi et al., 1984). Note that the second and third fragments are oriented such that BclI and NdeI sites are contiguous. The fourth fragment is the 3.0 KB ILT virus genomic EcoRI fragment containing the gB gene. All three genes are in the same transcriptional orientation as the UL43 gene.
EXAMPLES
Example 1
S-HVT-001
S-HVT-001 is a herpesvirus of turkeys (HVT) that contains the E. coli .beta.-galactosidase gene inserted into the unique long region of the HVT genome. The restriction enzyme map of HVT has been published (T. Igarashi, et al., 1985). This information was used as a starting point to engineer the insertion of foreign genes into HVT. The BamHI restriction map of HVT is shown in FIG. 1A. From this data, several different regions of HVT DNA into which insertions of foreign genes could be made were targeted. The foreign gene chosen for insertion was the E. coli .beta.-galactosidase (lacZ) gene, which we have used in PRV. The promoter was the PRV gpX promoter. The lacZ gene was inserted into the unique long region of HVT, specifically into the XhoI site in the BamHI #16 (3329 bp) fragment, and was shown to be expressed in an HVT recombinant by the formation of blue plaques using the substrate Bluogal.TM. (Bethesda Research Labs). Similarly, the lacZ gene has been inserted into the SalI site in the repeat region contained within the BamHI #19 (900 bp) fragment.
These experiments show that HVT is amenable to the procedures described within this application for the insertion and expression of foreign genes in herpesviruses. In particular, two sites for insertion of foreign DNA have been identified (FIGS. 1B and 1C).
Example 2
S-HVT-003
S-HVT-003 is a herpesvirus of turkeys (HVT) that contains the E. coli .beta.-galactosidase (lacZ) gene and the infectious bursal disease virus (IBDV) strain S40747 large segment of RNA (as a cDNA copy) inserted into the unique long region of the HVT genome. This IBDV DNA contains one open reading frame that encodes three proteins (5'VP2-VP4-VP3 3'), two of which are antigens to provide protection against IBDV infections of chickens. Expression of the genes for both .beta.-galactosidase and the IBDV polyprotein are under the control of the pseudorabies virus (PRV) gpX gene promoter. S-HVT-003, deposited under ATCC Accession No. VR 2178, was made by homologous recombination.
The IBDV genes were cloned by the cDNA CLONING PROCEDURE. Clones representing the genome of IBDV were screened by SOUTHERN BLOTTING OF DNA procedure against blots containing authentic IBDV RNA. Positive clones were then characterized by restriction mapping to identify groups of clones. Two such clones were identified, that together were found to represent the entire coding region of the IBDV large segment of RNA (3.3 kb dsRNA). One cDNA clone (2-84) contained an approximately 2500 base pair fragment representing the first half of the IBDV gene. The second clone (2-40) contained an approximately 2000 base pair fragment representing the distal half of the IBDV gene. Plasmid 2-84/2-40, representing the entire IBDV gene, was constructed by joining clone 2-84 and 2-40 at a unique PvuII site present in the overlapping sequences. The IBDV genome can be obtained from plasmid 2-84/2-40 as an approximately 3400 base pair SmaI to HpaI fragment. Confirmation of the nature of the proteins encoded by the IBDV gene was obtained by expressing the clone (2-84/2-40) in E. coli and detecting VP3 antigen using antiserum made against purified IBDV capsid proteins on Western blots. Applicants' sequence of the large DNA segment that encodes the IBDV antigens is given seq ID # 1. This sequence shows one open reading frame that will henceforth be referred to as the IBDV gene. The sequence of an Australian IBDV strain has been published which bears close homology to applicants' sequence (Hudson et al, 1986). Comparison of the amino acid differences between the two viruses revealed 29 amino acid changes within the 1012 amino acid coding region. There were only 3 amino acid differences deduced for VP4 and only 8 in VP3. In contrast, VP2 contained 18 amino acid changes, 14 of which were clustered between amino acids 139 to 332.
For insertion into the genome of HVT, the coding region for the IBDV gene was cloned between the PRV gpx promoter and the HSV TK poly-A signal sequence, creating plasmid 191-23. To aid in the identification of HVT recombinants made by homologous recombination containing the IBDV gene the gpX promoted IBDV fragment from plasmid 191-23 was inserted behind (in tandem to) a lacZ gene controlled by a gpX promoter. The resultant plasmid, 191-47, contains the E. coli lacZ gene and the IBDV gene under the control of individual PRV gpX promoters. In constructing plasmid 191-47, various DNA fragments were joined by recombinant DNA techniques using either naturally occurring restriction sites or synthetic linker DNA. Details concerning the construction of these genes contained in plasmid 191-47 can be seen in FIG. 2. The first segment of DNA (segment 1, FIG. 2) contains the gpX promoter region including the residues encoding the first seven amino acids of the gpX gene, and was derived from a subclone of the PRV BamHI #10 fragment as an approximately 800 base pair SalI to BamHI fragment. The second segment of DNA (segment 2, FIG. 2) contains the E. coli .beta.-galactosidase coding region from amino acid 10 to amino acid 1024 and was derived from the plasmid pJF751 (obtained from Jim Hoch, Scripps Clinic and Research Foundation) as an approximately 3300 base pair BamHI to BalI fragment followed by an approximately 40 base pair Ava I to Sma I fragment. The third segment of DNA (segment 3, FIG. 2) contains the gpX poly A signal sequence and was derived from a subclone of the PRV BamHI #7 fragment as an approximately 700 base pair NdeI to StuI fragment. Segment three was joined to segment two by ligating the NdeI end which had been filled in according to the POLYMERASE FILL-IN REACTION, to the SmaI site. The fourth segment of DNA (segment 4 FIG. 2) contains the gpX promoter (TATA box and cap site) and was derived from a subclone of the PRV BamHI #10 fragment as an approximately 330 base pair NaeI to AluI fragment. Additionally, segment four contains approximately 36 base pairs of HSV TK 5' untranslated leader sequence as a PstI to BglII fragment in which the PstI site has been joined to the AluI site through the use of a synthetic DNA linker (McKnight and Kingbury, 1982). DNA segments four through six were inserted as a unit into the unique Kpn I site of segment three which is located 3' of the gpX poly A signal sequence. The fifth segment of DNA (segment 5, FIG. 2) contains the entire coding region of the IBDV large segment of RNA (cDNA clone) as an approximately 3400 base pair SmaI to HpaI fragment. The SmaI site of segment five was fused to the BglII site of segment four which had been filled in according to the POLYMERASE FILL IN REACTION Expression of the IBDV gene (5' VP2-VP4-VP3 3'), is under the control of the gpX promoter (segment 4), but utilizes its own natural start and stop codons. The sixth segment of DNA (segment 6, FIG. 2) contains the HSV TK poly-A signal sequence as an approximately 800 base pair SmaI fragment (obtained from Bernard Roizman, Univ. of Chicago). The HpaI site of segment five was fused to the SmaI site of segment six through the use of a synthetic DNA liner.
In summary, the construct used to create S-HVT-003 (plasmid 191-47) contains (5' to 3') the PRV promoter, the gpX TATA box, the gpX cap site, the first seven amino acids of gpX, the E. coli .beta.-galactosidase (lacZ) gene, the PRV poly-A signal sequence, the PRV gpX promoter, the gpX TATA box, the gpX cap site, a fusion within the gpX untranslated 5' leader to the IBDV gene, IBDV start codon, a fusion within the IBDV untranslated 3' end to HSV TK untranslated 3' end, and the TK poly-A signal sequence. The cassette containing these genes was engineered such that it was flanked by two EcoRI restriction endonuclease sites. As a result, an approximately 9100 base pair fragment containing both lacZ gene and the IBDV gene can be obtained by digestion with EcoRI. Henceforth, the 9161 base pair EcoRI fragment will be referred to as the IBDV/lacZ cassette. The following procedures were used to construct S-HVT-003 by homologous recombination. The IBDV/lacZ cassette was inserted into the unique XhoI site present within a subclone of the HVT BamHI #16 fragment. To achieve this, the XhoI site was first changed to an EcoRI site through the use of an EcoRI linker. This site had previously been shown to be nonessential in HVT by the insertion of lacZ (S-HVT-001). It was also shown that the flanking homology regions in BamHI #16 were efficient in homologous recombination. Shown in FIG. 3, the genomic location of the BamHI #16 fragment maps within the unique long region of HVT. The complete nucleotide sequence of the approximately 3329 base pair BamHI #16 fragment is presented as seq ID #3. HVT DNA was prepared by the PREPARATION OF HERPESVIRUS DNA procedure. Cotransfections of HVT DNA and plasmid DNA into primary chick embryo fibroblast (CEF) cells were done according to the DNA TRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUS. The recombinant virus resulting from the cotransfection stock was purified by three successive rounds of plaque purification using the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS procedure. When 100% of the plaques were blue, the DNA was analyzed for the presence of the IBDV gene by the SOUTHERN BLOTTING OF DNA procedure. Southern blots, probing EcoRI digested S-HVT-003 DNA with an IBDV specific nick translated probe (plasmid 2-84/2-40), confirmed the presence of the 9100 base pair EcoRI fragment. This result confirmed that S-HVT-003 contained both the lacZ gene and the IBDV gene incorporated into its genome. Additional Southern blots, using a probe specific for BamHI #16, confirmed that the homologous recombination occurred at the appropriate position in BamHI #16 and that no deletions were created. No differences in the growth of S-HVT-003 compared to wild type virus (S-HVT-000) were observed in vitro.
Expression of IBDV specific proteins from S-HVT-003 were assayed in vitro using the WESTERN BLOTTING PROCEDURE. Cellular lysates were prepared as described in PREPARATION OF HERPESVIRUS CELL LYSATES. Briefly, the proteins contained in the cellular lysates of S-HVT-003 were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with either an antiserum made against denatured purified IBDV capsid proteins or antiserum made against a synthetic peptide corresponding to a predicted imuno dominant region of the IBDV 40 kd (VP2) capsid protein. The filters were washed and treated with �.sup.125 I! protein A to detect the position of the bound antibodies. FIG. 4 shows the results obtained using the antiserum made against denatured purified IBDV capsid proteins, which have been shown by the applicants to react primarily with VP3 (32 kd protein). As seen, S-HVT-003 produces a protein which is immunologically indistinguishable from the authentic VP3 protein from intact IBDV virions. Moreover, the polyprotein appears to be processed correctly, producing a VP3 species that comigrates with the authentic VP3 protein. Recent evidence using an Australian IBDV stain indicates that VP4 is involved in the processing of the precursor polyprotein into mature VP2 and VP3 protein species (Jagadish, et al., 1988). FIG. 5 shows the results obtained using a rabbit antiserum raised against a synthetic peptide that is homologous to a 14 amino acid region of the IBDV VP2 (40 kd) capsid protein. As seen, S-HVT-003 produces a protein that is immunologically indistinguishable from the authentic viral VP2 protein. In addition, the VP2 protein produced from S-HVT-003 comigrates with the 40 kd species of VP2 isolated from intact IBDV virions. This species represents a major component of infectious (complete) viral particles.
In summary, analysis of the expression of IBDV specific proteins from S-HVT-003 has shown that the polyprotein is processed in CEF cell culture, producing proteins of the appropriate size that react to immunological reagents specific for either VP2 or VP3 proteins on Western blots.
The following set of experiments was carried out in chickens to analyze the in vivo expression of the IBDV genes contained within S-HVT-003 as determined by seroconversion data, serum neutralization results, and protection from IBDV challenge.
The first experiment was designed to show the seroconversion of chickens to IBDV upon being vaccinated with S-HVT-003. Eleven 11-week-old chickens, seronegative to HVT and IBDV were obtained from SPAFAS Inc. Six birds were vaccinated subcutaneously in the abdominal region with 0.5 ml of a cellular suspension of CEF cells containing S-HVT-003 (40,000 PFU/ml). Serum samples were obtained every seven days for eight weeks for all birds in this study. On day 28 (4th week), three of these birds received a boost of S-HVT-003, while the other three birds received 0.5 ml of an inactivated IBDV vaccine inoculated subcutaneously in the cervical region. Three additional birds were given only the inactivated vaccine on day 28. Two birds served as contact controls and received no vaccinations. On day 56, all birds were sacrificed and necropsied. Table 1 show the results of the serum neutralization assay against IBDV. No detectable SN activity was observed in the birds given only S-HVT-003. Additionally, only one of the three birds that were given only the inactivated vaccine demonstrated low but detectable SN activity. SN titers were also detected in one of the three birds that received the S-HVT-003 followed by the inactivated IBDV vaccine boost; these titers were at a much higher level than with the inactivated IBDV vaccine alone. These results suggest that S-HVT-003 is priming the chicken for a secondary response against IBDV. In vitro analysis of the serum samples by WESTERN BLOTTING confirmed the seroconversion of the chickens to IBDV upon vaccination with S-HVT-003 both prior to and after boosts administered on day 28.
TABLE 1______________________________________Vaccine DAYGroup Bird No. 28 31 35 38 42 49______________________________________HVT-003 265 <2 <2 <2 <2 <2 <2HVT-003 266 <2 <2 <2 <2 <2 <2 267 <2 <2 <2 <2 <2 <2HVT-003 260 <2 <2 <2 <2 <2 <2IBDV.sup.a 264 <2 <2 <2 1:64 1:256 1:512 269 <2 <2 <2 <2 <2 <2C 261 <2 <2 <2 <2 <2 <2IBDV.sup.a 262 <2 <2 <2 <2 1:4 1:4 263 <2 <2 <2 <2 <2 <2C 270 <2 <2 <2 <2 <2 <2 271 <2 <2 <2 <2 <2 <2______________________________________ .sup.a Commercial
In the second experiment, twenty five 1-day old SPF chicks were vaccinated with S-HVT-003 (20 with 0.2 ml subcutaneously and 5 by bilateral eyedrop). Twenty chicks were kept as controls. On days four and seven postinfection, five vaccinates and two control birds were bled, sacrificed and their spleens removed for virus isolation. Spleen cell suspensions were made by standard method, and .noteq.1.times.10.sup.6 cells in 3 ml of chick embryo fibroblast (CEF) growth media were inoculated directly onto secondary cells. Cultures were incubated for 6-7 days and then scored for cytopathic effects (CPE) as determined by observing cell morphology. The cultures were passed a second time, and again scored for CPE. The results are shown in Table 2. All nonvaccinated control birds remained negative for HVT for both day 4 and 7 spleen cell isolations. Four out of the five birds vaccinated with S-HVT-003 were positive for HVT at day 4 for both the first and second passages. One bird did not produce virus, this may represent a vaccination failure. Five out of five birds were positive for HVT on day 7 at both passage one and two. Overall, the vector recovery experiment demonstrates that S-HVT-003 replicates as well as wild type HVT virus in vivo and that insertion of the IBDV/lacZ cassette into the XhoI site of BamHI #16 does not result in detectable attenuation of virus. Subsequent experiments examining the recovered virus by the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS procedure confirmed the in vivo stability of S-HVT-003, by demonstrating .beta.-galactosidase expression in 100% of the viruses.
TABLE 2______________________________________Harvest Date Day 4 Day 7Sample P1 P2 P1 P2______________________________________N1 - -N2 - -N3 - -N4 - -T1 - -T2 2+ 2+T3 2+ 2+T4 + 4+T5 3+ 3+T6 2+ contaminatedT7 + 5+T8 + 5+T8 + 5+T9 + 5+ T10 + 5+______________________________________ N = control, T = vaccinated CPE ranged from negative (-) to 5
At days 0, 4, 7, 14, 21, and 27 postinfection, blood samples were obtained from the rest of the chickens for determining serum ELISA titers against IBDV and HVT antigens as well as for virus neutralizing tests against IBDV. Additionally, at 21 days postinfection five control and fourteen vaccinated chicks were challenged with virulent IBDV by bi-lateral eyedrop (10.sup.3.8 EID.sub.50). All birds were sacrificed 6-days post challenge and bursa to body weight ratios were calculated. A summary of the results is shown in tables X and XI, respectively. As presented in Table 3, no antibodies were detected against HVT antigens by ELISA prior to 21-27 days post vaccination. In chickens, the immune response during the first two weeks post hatch is both immature and parentally suppressed, and therefore these results are not totally unexpected. In contrast, IBDV ELISA's were negative up to day 21 postvaccination, and were only detectable after challenge on day 27. The ELISA levels seen on day 27 postvaccination indicate a primary response to IBDV. Table 3I comparing the Bursa-to-Body weight ratios for challenged controls and vaccinated/challenged groups show no significant differences. Vaccination with S-HVT-003 under these conditions did not prevent infection of the vaccinated birds by IBDV challenge, as indicated by the death of four vaccinated birds following challenge.
TABLE 3______________________________________ ELISA VNSample Group HVT IBDV IBDV______________________________________C-0 (n = 3) 0 0 <100C-4 (n = 2) 0 0 ndT-4 (n = 5) 0 0 ndC-7 (n = 2) 0 0 <100T-7 (n = 5) 0 0 <100C-14 (n = 5) 0 0 ndT-14 (n = 14) 0 0 <100C-21 (n = 5) 0 0 ndT-21 (n = 14) 1 0 <100C-27 (n = 5) 0 0 ndCC-27 (n = 5) 0 5 ndCT-27 (n = 10) 3.2 2 nd______________________________________ C = control, T = vaccinated, CC = challenged control, CT = Challenged & vaccinated. ELISA titers are GMTs and they range from 0-9.
TABLE 4______________________________________Sample Group Body wt. Bursa wt. BBR______________________________________Con. (n = 5) 258.8 1.5088 0.0058Chall. 209 0.6502 0.0031Con. (n = 5)Chall. 215.5 0.5944 0.0027Treated (n = 10)______________________________________ Values are mean values. Body weights are different in control group because challenged birds did not feed well. Four challengedtreated birds died.
A third experiment was conducted repeating Experiment 2 but using immunologically responsive chicks (3 weeks of age). Six three week old SPF leghorn chickens were vaccinated intraperitoneally with 0.2 ml of S-HVT-003 (one drop in each eye). Serum samples were obtained every seven days for six-weeks and the birds were challenged with the virulent USDA standard challenge IBDV virus on day 43 postvaccination. Six days post challenge, the control, vaccinated-challenged, and challenged groups were sacrificed and bursas were harvested for probing with anti-IBDV monoclonal antibodies (MAB) (provided by Dr. David Snyder, Virginia-Maryland Regional College of Veterinary Medicine). Bursal homogenates were prepared by mixing 1 ml of 0.5% NP40 with one bursa. Bursa were then ground and briefly sonicated. Supernatants from the homogenates were reacted with the R63 MAB which had been affixed to 96-well Elisa plates via a protein A linkage. After incubation, a biotin labeled preparation of the R63 MAB was added. After washing, an avidin-horse radish peroxidase conjugate was added and incubated. Tests were developed with Tris-malcate buffer (TMB)+H.sub.2 O.sub.2 substrate. The test results are presented in Table 5. The data show the presence of high levels of IBDV antigen in all bursa in the vaccinate-challenged group and in the challenged group. No IBDV antigen was detected in the controls. IBDV specific antigen could be detected at dilutions of over 1/1000, and there does not appear to be differences between vaccinated and non-vaccinated challenged groups. HVT titers as determined by ELISA were first detectable at day 7 in four out of the six birds vaccinated. By day 14, six out of six vaccinated birds showed titers to HVT. All six birds continued to show HVT titers throughout the experiment. No IBDV SN titers were seen prior to the challenge. In contrast, analysis of these same serum samples by the WESTERN BLOTTING procedure demonstrated the seroconversion of chickens vaccinated with S-HVT-003 to IBDV prior to administration of the virus challenge. The level of response, however, remains small unless boosted by challenge. Comparison between the vaccinated/challenged and challenged only groups clearly demonstrates that the level of reactivity by Western blots is much higher in the vaccinated/challenged group. These results show that S-HVT-003 is seroconverting vaccinated birds to IBDV, and suggest that the level of IBDV specific expression are not high enough to induce a neutralizing response in the birds.
S-HVT-003 shows the merit of the vaccine approach the applicants have invented. HVT has been engineered to simultaneously express the foreign antigens (.beta.-galactosidase and IBDV antigens) that are recognized in the host by an immune response directed to these proteins. Applicants' invention will enable progression towards a product based on this technology.
TABLE 5______________________________________Serology: Herpes/IBDV ELISA titerBleed DateBird # 11/3 11/10 11/14 11/24 12/1 12/8 12/15 12/22______________________________________Vaccinated Challenged221 0/0 7/0 5/0 6/0 5/0 5/0 5/0 3/341 0/0 4/0 4/0 1/0 1/0 1/0 1/0 1/342 0/0 3/0 2/0 1/0 5/0 5/0 5/0 3/243 0/0 0/0 5/0 5/0 5/0 5/0 3/0 3/244 0/0 1/0 5/0 1/0 2/0 1/0 1/0 2/445 0/0 0/0 1/0 1/0 1/0 1/0 1/0 1/3Control28 0/0 0/038 0/0 0/073 0/0 0/075 0/0 0/0Challenged only40 0/0 0/374 0/0 0/539 0/0 0/372 0/0 0/3______________________________________ Maximum titer level is 9
Example 3
S-HVT-004
S-HVT-004 is a recombinant herpesvirus of turkeys that contains the Marek's disease virus (MDV) glycoprotein A (gpA) gene inserted into the long unique region, and the .beta.-galactosidase (lacZ) gene also inserted in the long unique region. The MDV antigen is more likely to elicit the proper antigentic response than the HVT equivalent antigen.
The MDV gpA gene was cloned by standard DNA cloning gpA procedures. An EcoRI restriction fragment had been reported to contain the MDV gpA gene (Isfort et al., 1984) and this fragment was identified by size in the DNA clones. The region of the DNA reported to contain the gpA gene was sequenced by applicants and found to contain a glycoprotein gene as expected. The DNA from this gene was used to find the corresponding gene in HVT by the SOUTHERN BLOTTING OF DNA procedure, and a gene in HVT was identified that contained a very similar sequence. This gene is the same gene previously called gpA (Isfort et al., 1984).
For insertion into the genome of HVT, the MDV gpA gene was used intact because it would have good herpesvirus signal sequences already. The lacZ gene was inserted into the XhoI fragment in BamHI fragment #16, and the MDV gpA gene was inserted behind lacZ as shown in FIGS. 6A and 6B. Flanking regions in BamHI #16 were used for the homologous recombination. HVT DNA and plasmid DNA were co-transfected according to the DNA TRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUS procedure into primary chick embryo fibroblast (CEF) cells. The virus from the transfection stock was purified by successive plaque purifications using the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS procedure. At the end of this procedure, when 100% of the plaques were blue, the DNA was analyzed for the presence of the MDV gpA gene. S-HVT-004 is a recombinant virus that contains both the .beta.-galactosidase gene and the MDV gpA gene incorporated into the genome.
FIG. 6C shows the structure of S-HVT-004.
Example 4
Newcastle Disease Virus
Newcastle disease virus (NDV) is closely related to PI-3 in overall structure. We have engineered the hemagglutinin (HN) and fusion (F) genes of PI-3 for expression in IBR (ref). Similarly we have cloned the hemagglutinin (HN) and fusion (F) genes from NDV for use in the herpesvirus delivery system (Herpesvirus of turkeys, HVT).
The procedures that we have utilized for construction of herpesvirus control sequences for expression have been applied to NDV.
Infectious Bronchitis Virus
Infectious bronchitis virus (IBV) is a virus of chickens closely related in overall structure to TGE. We have engineered the major neutralizing antigen of TGE for expression in PRV (ref). Similarly we have cloned the major neutralizing antigens from three strains of IBV: Massachusetts (SEQ ID NO: 14), Connecticut (SEQ ID NO: 16), and Arkansas-99 (SEQ ID NO: 18) for use in a herpesvirus delivery system (HVT).
The procedures that we have utilized for the construction of herpesvirus control sequences for expression have been applied to IBV.
Example 5
S-HVT-045
S-HVT-045 is a recombinant herpesvirus of turkeys that contains the Marek's disease virus (MDV) glycoprotein B (gpB) gene inserted into the short unique region. The MDV antigen is more likely to elicit the proper antigenic response than the HVT equivalent antigen. S-HVT-045 has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
The MDV gpB gene was cloned by standard DNA cloning procedures. The MDV gpB gene was localized to a 3.9 kb EcoRI-SalI fragment using an oligonucleotide probe based on the HSV gB sequence in a region found to be conserved among known herpesvirus gB genes. The restriction map 3.9 kb EcoRI-SalI fragment is similar to the published map (Ross et al., 1989).
For insertion into the HVT genome, the MDV gpB was used intact because it would have good herpesvirus signal sequences already. The MDV gpB gene was inserted into a cloned 17.15 kb BamHI-EcoRI fragment derived from the HVT BamHI #1 fragment. The site used for insertion was the StuI site within HVT US2, previously utilized for the construction of S-HVT-012. The site was initially altered by insertion of a unique HindIII linker, and the MDV gpB gene was inserted by standard DNA cloning procedures. Flanking regions in the 17.15 kb BamHI-EcoRI fragment were used, together with the remaining cloned HVT fragments using the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUSES FROM OVERLAPPING SUBGENOMIC FRAGMENTS. The virus obtained from the transfection stock was plaque purified and the DNA was analyzed for the presence of the MDV gpB gene. S-HVT-045 is a recombinant virus that contains the MDV gpB gene incorporated into the genome at the StuI site in HVT US2 gene.
Testing of Recombinant S-HVT-045
Two studies were conducted to demonstrate the effectiveness of these recombinant HVT/MDV viruses in protecting against challenge with virulent Marek's disease virus. In Study A, one-day-old specific pathogen free (SPF) chicks were vaccinated with either S-HVT-045 or S-HVT-046. Seven days post-vaccination, vaccinated chicks, and non-vaccinated, control chicks were challenged with the highly virulent MD-5 strain of Marek's disease virus. Following a 6-week post-challenge observation period for clinical signs typical of Marek's disease, all chicks were necropsied and examined for lesions diagnostic of Marek's disease. The results, in Table 6, show that both recombinant viruses gave complete protection against a challenge that caused Marek's disease in 90% of non-vaccinated control chicks.
In a second study, one-day-old chicks were vaccinated either with S-HVT-045 or S-HVT-047. A third group of chicks were vaccinated with a USDA-licensed, conventional vaccine comprised of HVT and SB-1 viruses. Five days post-vaccination, the vaccinated chicks and a group of non-vaccinated, control chicks were challenged with virulent Marek's virus, strain RBLB. The chicks were observed for 8 weeks for clinical signs of Marek's disease, then necropsied and observed for Marek's lesions. This study demonstrated the ability of HVT-045 and HVT-047 to provide 100% protection against challenge (Table 1). The commercial vaccine gave 96% protection, and 79% of the non-vaccinated chicks developed Marek's disease.
TABLE 6______________________________________EFFICACY OF RECOMBINANT HVT/MDV VIRUSESTO PROTECT SUSCEPTIBLE CHICKSAGAINST VIRULENT MAREK'S DISEASE VIRUS Marek's ProtectionVaccine Group MD-5 Challenge RB1B Challenge______________________________________S-HVT-045 20/20 24/24S-HVT-046 20/20 Not TestedS-HVT-047 Not Tested 24/24HVT.sup.a Not Tested 24/25Controls 2/20 5/24______________________________________ .sup.a Commercial
Example 6
S-HVT-012
S-HVT-012 is a recombinant herpesvirus of turkeys that contains the E. coli .beta.-galactosidase (lacZ) gene inserted into the short unique region. The lacZ gene was used to determine the viability of this insertion site in HVT �ATCC F-126 ("Calnek")!. S-HVT-012 has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
For insertion into the genome of HVT, the .beta.-galactosidase gene was introduced into the unique StuI site of the cloned EcoRI fragment #7 of HVT, i.e., the fragment containing the StuI site within the US2 gene of HVT (as described in Methods and Materials). Flanking regions of EcoRI fragment #7 were used for homologous recombination. HVT DNA and plasmid DNA were co-transfected according to the DNA TRANSFECTION FOR GENERATING RECOMBINANT VIRUS procedure into primary chick embryo fibroblast (CEF) cells. A blue virus obtained from the transfection stock was purified by successive plaque purifications using the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS procedure. At the end of this procedure, when 100% of the plaques were blue, the DNA was analyzed for the presence of the lacZ gene. S-HVT-012 is a recombinant virus that contains the lacZ gene incorporated into the genome at the StuI site within the US2 gene of HVT.
Testing of Recombinant S-HVT-012
S-HVT-012 may be formulated as a vaccine in the same manner as S-HVT-045. When administered to chickens, such a vaccine provides protection against Marek's disease virus.
Example 7
Sites for Insertion of Foreign DNA into HVT
In order to define appropriate insertion sites, a library of HVT BamHI and EcoRI restriction fragments was generated. Several of these restriction fragments (BamHI fragments #16 and #13, and EcoRI fragments #6, #7, and #9 (see FIG. 1)) were subjected to restriction mapping analysis. One unique restriction site was identified in each fragment as a potential insertion site. These sites included XhoI in BamHI fragments #13 and #16, and EcoRI fragment #9 and SalI in EcoRI fragment #6 and StuI in EcoRI fragment #7. A .beta.-galactosidase (lacZ) marker gene was inserted in each of the potential sites. A plasmid containing such a foreign DNA insert may be used according to the DNA COTRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUSES to CONSTRUCT a HVT containing the foreign DNA. For this procedure to be successful it is important that the insertion site be in a region non-essential to the replication of the HVT and that the site be flanked with HVT DNA appropriate for mediating homologous recombination between virus and plasmid DNAs. The plasmids containing the lacZ marker gene were utilized in the DNA COTRANSFECTION FOR GENERATING RECOMBINANT HERPESVIRUSES. The generation of recombinant virus was determined by the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS. Three of the five sites were successfully used to generate a recombinant virus. In each case the resulting virus was easily purified to 100%, clearly defining an appropriate site for the insertion of foreign DNA. The three homology vectors used to define these sites are described below.
Example 7A
Homology Vector 172-29.31
The homology vector 172-29.31 contains the HVT BamHI #16 fragment and is useful for the insertion of foreign DNA into HVT. Plasmid 172-29.31 contains a unique XhoI restriction site into which foreign DNA may be cloned. We have demonstrated that the XhoI site in homology vector 172-29.31 may be used to insert foreign DNA into HVT by the construction of at least three recombinant HVT (see examples 1-3).
The homology vector 172-29.31 was further characterized by DNA sequence analysis. The complete sequences of the BamHI #16 fragment was determined. Approximately 2092 base pairs of the adjacent BamHI #13 fragment was also determined (see SEQ ID 3). This sequence indicates that the open reading frame coding for HVT glycoprotein A (gA) spans the BamHI #16--BamHI #13 junction. The HVT gA gene is homologous to the HSV-1 glycoprotein C (gC). The XhoI site interrupts an ORF which lies directly upstream of the HVT gA gene. This ORF shows amino acid sequence homology to the PRV p43 and the VZV gene 15. The PRV and VZV genes are the homologues of HSV-1 UL43. Therefore we have designated this ORF as HVT UL43. It should be noted that the HVT UL43 does not exhibit direct homology to HSV-1 UL43. Although HVT UL43 is located upstream of the HVT gC homologue it is encoded on the same DNA strand as HVT gA, where as the HSV-1 UL43 is on the opposite strand relative to HSV-1 gC. The XhoI site interrupts UL43 at approximately amino acid 6, suggesting that the UL43 gene is non-essential for HVT replication.
Example 7B
Homology Vector 435-47.R17
The homology vector 435-47.R17 contains the HVT EcoRI #7 fragment and is useful for the insertion of foreign DNA into HVT. Plasmid 435-47.R17 contains a unique HindIII restriction site into which foreign DNA may be cloned. The HindIII restriction site in plasmid results from the insertion of a HindIII linker into the naturally occurring StuI site of EcoRI fragment #7. We have demonstrated that the HindIII site in homology vector 435-47.R17 may be used to insert foreign DNA into HVT by the construction of at least 25 recombinant HVT.
DNA sequence analysis at the StuI indicated that this fragment contains open reading frames coding for US10, US2, and US3. The StuI site interrupts US2 at approximately amino acid 124, suggesting that the US2 gene is non-essential for HVT replication.
Example 7C
Homology Vector 172-63.1
The homology vector 172-63.1 contains the HVT EcoRI #9 fragment and is useful for the insertion of foreign DNA into HVT. Plasmid 172-63.1 contains a unique XhoI restriction site into which foreign DNA may be cloned. We have demonstrated that the XhoI site in homology vector 172-63.1 may be used to insert foreign DNA into HVT by the construction of S-HVT-014 (see example 8).
Example 8
S-HVT-014
S-HVT-014 is a recombinant herpesvirus of turkeys that contains the E. coli .beta.-galactosidase (lacZ) gene inserted into the long unique region. The lacZ gene was used to determine the viability of this insertion site in HVT �ATCC F-126 ("Calnek")!.
For insertion into the genome of HVT, the .beta.-galactosidase gene was introduced into the unique XhoI site of the cloned EcoRI fragment #9 (as described in Methods and Materials). Flanking regions of EcoRI fragment #8 were used for homologous recombination. HVT DNA and plasmid DNA were co-transfected according to the DNA TRANSFECTION FOR GENERATING RECOMBINANT VIRUS procedure into primary chick embryo fibroblast (CEF) cells. A blue virus obtained from the transfection stock was purified by successive plaque purifications using the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS procedure. At the end of this procedure when 100% of the plaques were blue. S-HVT-014 is a recombinant virus that contains the lacZ gene incorporated into the genome at the XhoI site within the EcoRI #9 fragment of HVT.
Testing of Recombinant S-HVT-014
S-HVT-014 may be formulated as a vaccine in the same manner as S-HVT-045. When administered to chickens, such a vaccine provides protection against Marek's disease virus.
Example 9
S-HVT-005
S-HVT-005 is a recombinant herpesvirus of turkeys that contains the E. coli .beta.-galactosidase (lacZ) gene inserted into the long unique region. The lacZ gene was used to determine the viability of this insertion site in HVT �ATCC F-126 ("Calnek")!.
For insertion into the genome of HVT, the .beta.-galactosidase gene was introduced into an approximately 1300 base pair deletion of the XhoI #9 fragment of HVT. The deletion which lies between the unique MluI and EcoRV sites removes the complete coding region of the HVT gA gene (see SEQ ID 3). Flanking regions of XhoI fragment #9 were used for homologous recombination. HVT DNA and plasmid DNA were co-transfected according to the DNA TRANSFECTION FOR GENERATING RECOMBINANT VIRUS procedure into primary chick embryo fibroblast (CEF) cells. A blue virus obtained from the transfection stock was purified by successive plaque purifications using the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS procedure. At the end of this procedure, when 100% of the plaques were blue, the DNA was analyzed for the presence of the lacZ gene. S-HVT-005 is a recombinant virus that contains the lacZ gene incorporated into the genome at in place of the deleted gA gene of HVT.
Testing of Recombinant S-HVT-005
S-HVT-005 may be formulated as a vaccine in the same manner as S-HVT-045. When administered to chickens, such a vaccine provides protection against Marek's disease virus.
Example 10
Marek's Disease Vaccines
Recombinant HVT expressing glycoproteins from MDV make superior vaccines for Marek's Disease. We have constructed several recombinant HVT expressing MDV glycoproteins S-HVT-004 (example 3), S-HVT-005 (example 9), S-HVT-045 (example 5), S-HVT-046 (example 10A), S-HVT-047 (example 10B), S-HVT-062 (example 10C).
Example 10A
S-HVT-046
S-HVT-046 is a recombinant herpesvirus of turkeys that contains the Marek's disease virus (MDV) glycoprotein B (gB) and glycoprotein A (gA) genes inserted into the short unique region. The MDV genes are inserted in the same transcriptional orientation as the US2 gene. The MDV antigens are more likely to elicit the proper antigenic response than the HVT equivalent antigen.
S-HVT-046 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 456-17.22 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Example 10B
S-HVT-047
S-HVT-047 is a recombinant herpesvirus of turkeys that contains the MDV gB and gA genes inserted into the short unique region. The MDV genes are inserted in the opposite transcriptional orientation as the US2 gene. The MDV antigens are more likely to elicit the proper antigenic response than the HVT equivalent antigen.
S-HVT-047 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 456-17.18 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Example 10C
S-HVT-062
S-HVT-062 is a recombinant herpesvirus of turkeys that contains the MDV gB, glycoprotein D (gD) and gA genes inserted into the short unique region. The MDV genes are inserted in the same transcriptional orientation as the US2 gene. The MDV antigens are more likely to elicit the proper antigenic response than the HVT equivalent antigen. S-HVT-062 has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A. under ATCC Accession No. VR 2401 on Feb. 23, 1993.
S-HVT-062 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 566-41.5, with BamHI and HindIII, and 456-17.22 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Testing of Recombinant HVT Expressing MDV Antigens
Two studies were conducted to demonstrate the effectiveness of these recombinant HVT/MDV viruses in protecting against challenge with virulent Marek's disease virus. In Study 1, one-day-old specific pathogen free (SPF) chicks were vaccinated with either S-HVT-045, S-HVT-046, or S-HVT-047. Five days post-vaccination, vaccinated chicks, and non-vaccinated, control chicks were challenged with MDV. Following a 6-week post-challenge observation period for clinical signs typical of Marek's disease, all chicks were necropsied and examined for lesions diagnostic of Marek's disease. The results, in Table 7, show these recombinant viruses gave complete protection against a challenge that caused Marek's disease in 84% of non-vaccinated control chicks.
In the second study, one-day-old chicks were vaccinated with S-HVT-062. Two more groups of chicks were vaccinated with a USDA-licensed, conventional vaccines comprised of HVT and a combination HVT and SB-1 viruses. Five days post-vaccination, the vaccinated chicks and a group of non-vaccinated, control chicks were challenged with MDV. The chicks were observed for 8 weeks for clinical signs of Marek's disease, then necropsied and observed for Marek's lesions. This study demonstrated the ability of S-HVT-062 to provide 100% protection against challenge (Table 7). The commercial vaccines gave 81% and 95% protection, respectively and 100% of the non-vaccinated chicks developed Marek's disease.
TABLE 7______________________________________EFFICACY OF RECOMBINANT HVT/MDV VIRUSESAGAINST VIRULENT MAREK'S VIRUS CHALLENGEStudy Vaccine Group Dose.sup.a Protection.sup.b______________________________________1 S-HVT-045 2.2 .times. 10.sup.3 24/24 (100%)1 S-HVT-046 2.2 .times. 10.sup.3 20/20 (100%)1 S-HVT-047 2.2 .times. 10.sup.3 24/24 (100%)1 Controls 7/44 (16%)1 HVT/SB-1 24/25 (96%)2 S-HVT-062 7.5 .times. 10.sup.2 32/32 (100%)2 S-HVT-062 1.5 .times. 10.sup.3 22/22 (100%)2 Controls 0/20 (0%)2 HVT.sup.c 7.5 .times. 10.sup.2 17/21 (81%)2 HVT/SB-1.sup.c 7.5 .times. 10.sup.2 21/22 (95%)______________________________________ .sup.a PFU/0.2 ml. .sup.b No. protected/Total; Challenge 5 days postvaccination. .sup.c Commercial vaccine.
Example 11
Bivalent Vaccines Against Newcastle Disease and Marek's Disease
Recombinant HVT expressing proteins from NDV make bivalent vaccines protecting against both Marek's Disease and Newcastle disease. We have constructed several recombinant HVT expressing NDV proteins S-HVT-007 (example 11A), S-HVT-048 (example 11B), S-HVT-049 (example 1C), S-HVT-050 (example 1D), and S-HVT-106 (example 11E).
Example 11A
S-HVT-007
S-HVT-007 is a recombinant herpesvirus of turkeys that contains a hybrid E. coli lacZ NDV HN hybrid protein gene under the control of the PRV gX promoter and the NDV F gene under the control of the HSV-1 a 4 promoter inserted into the long unique region. The NDV genes are inserted in the same transcriptional orientation as the UL43 gene.
To construct S-HVT-007 HVT DNA and the plasmid 255-18.B16 were co-transfected according to the DNA TRANSFECTION FOR GENERATING RECOMBINANT VIRUS procedure into primary chick embryo fibroblast (CEF) cells. A blue virus obtained from the transfection stock was purified by successive plaque purifications using the BLUOGAL SCREEN FOR RECOMBINANT HERPESVIRUS procedure. At the end of this procedure, when 100% of the plaques were blue.
Example 11B
S-HVT-048
S-HVT-048 is a recombinant herpesvirus of turkeys that contains the MDV gB and gA genes and the NDV F gene under the control of the HCMV immediate early promoter inserted into the short unique region. The MDV and NDV genes are inserted in the same transcriptional orientation as the US2 gene.
S-HVT-048 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 535-70.3 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Example 11C
S-HVT-049
S-HVT-049 is a recombinant herpesvirus of turkeys that contains the MDV gB and gA genes and the NDV HN gene under the control of the PRV gX promoter inserted into the short unique region. The MDV and NDV genes are inserted in the same transcriptic entation as the US2 gene.
S-HVT-049 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 549-62.10 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Example 11D
S-HVT-050
S-HVT-050 is a recombinant herpesvirus of turkeys that contains the MDV gB and gA genes and the NDV HN and F genes. The NDV genes are under the control of the PRV gX and HCMV immediately promoters respectively. All four genes are inserted into the short unique region in the same transcriptional orientation as the US2 gene.
S-HVT-050 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 549-24.15 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis. S-HVT-050 has been deposited pursuant to the Budapest Treaty on the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Patent Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852 U.S.A.
Example 11E
S-HVT-106
S-HVT-106 is a recombinant herpesvirus of turkeys that contains the MDV gA, gB, gD genes and the NDV HN and F genes. The NDV genes are under the control of the PRV gX and HCMV immediately promoters respectively. All five genes are inserted into the short unique region in the same transcriptional orientation as the US2 gene.
S-HVT-050 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 633-13.27 uncut.
Testing of Recombinant HVT Expressing NDV Antigens
Two studies were conducted to demonstrate the effectiveness of these recombinant HVT/MDV/NDV viruses in protecting against challenge with virulent Newcastle and Marek's disease viruses. In Study 1, one-day-old specific pathogen free (SPF) chicks were vaccinated with either S-HVT-048, S-HVT-049, S-HVT-050, or a USDA-licensed, conventional vaccine comprised of NDV B1/B1 virus. Three weeks post-vaccination, vaccinated chicks, and non-vaccinated, control chicks were challenged with NDV. Birds were then observed for clinical signs of disease. The results, in Table 8, show these recombinant viruses (S-HVT-048 and S-HVT-050) gave complete against a challenge that caused Newcastle disease in 100% of non-vaccinated control chicks. Recombinant virus S-HVT-049 gave partial protection against Newcastle disease.
In the second study, one-day-old chicks were vaccinated with S-HVT-050. Two more groups of chicks were vaccinated with a USDA-licensed, conventional vaccines comprised of HVT and a combination HVT and SB-1 viruses. Five days post-vaccination, the vaccinated chicks and a group of non-vaccinated, control chicks were challenged with MDV. The chicks were observed for 8 weeks for clinical signs of Marek's disease, then necropsied and observed for Marek's lesions. This study demonstrated the ability of S-HVT-050 to provide protection greater than the commercial Marek's disease vaccines.
TABLE 8______________________________________EFFICACY OF RECOMBINANT HVT/MDV/NDV VIRUSESAGAINST VIRULENT NEWCASTLE ANDMAREK'S DISEASE VIRUS CHALLENGEVaccine Protection (%)Study Group Dose.sup.a NDV.sup.b MDV.sup.c______________________________________1 S-HVT-048 4.0 .times. 10.sup.4 19/19 (100)1 S-HVT-049 3.0 .times. 10.sup.4 4/20 (20)1 S-HVT-050 1.5 .times. 10.sup.4 20/20 (100)1 Controls 0/20 (0)1 NDV B1/B1.sup.d 18/18 (100)2 S-HVT-050 7.5 .times. 10.sup.2 13/14 (93)2 S-HVT-050 1.5 .times. 10.sup.3 16/17 (94)2 Controls 5/23 (22)2 HVT.sup.d 20/26 (77)2 HVT/SB-1.sup.d 10/12 (83)______________________________________ .sup.a PFU/0.2 ml. .sup.b No. protected/Total; Challenge 3 weeks postvaccination. .sup.c No. protected/Total; Challenge 5 days postvaccination. .sup.d Commercial vaccine.
Example 12
Bivalent Vaccines Against Infectious Laryngotracheitis and Marek's Disease
Recombinant HVT expressing glycoproteins from ILT virus make bivalent vaccines protecting against both Marek's disease and infectious laryngotracheitis. We have constructed several recombinant HVT expressing ILT virus glycoproteins S-HVT-051 (example 12A), S-HVT-052 (example 12B), and S-HVT-104 (example 11C).
Example 12A
S-HVT-051
S-HVT-051 is a recombinant herpesvirus of turkeys that contains the ILT virus gB gene inserted into the short unique region. The ILT gene is inserted in the same transcriptional orientation as the US2 gene.
S-HVT-051 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 528-11.34 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Example 12B
S-HVT-052
S-HVT-052 is a recombinant herpesvirus of turkeys that contains the ILT virus gD gene inserted into the short unique region. The ILT gene is inserted in the opposite transcriptional orientation as the US2 gene.
S-HVT-052 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 437-26.26 with BamHI and HindIII, and 528-03.37 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Example 12C
S-HVT-104
S-HVT-104 is a recombinant herpesvirus of turkeys that contains six foreign genes. The MDV gA, gB, and gD genes are inserted in the unique short region in the same transcriptional orientation as the US2 gene. An E. coli lacZ marker gene and the ILT gB and gD genes are inserted in BamHI #16 region in the same transcriptional orientation as the UL43 gene.
To construct S-HVT-104 DNA from S-HVT-062 and the plasmid 634-29.16 were co-transfected according to the DNA TRANSFECTION FOR GENERATING RECOMBINANT VIRUS procedure into primary chick embryo fibroblast (CEF) cells.
Testing of Recombinant HVT Expressing ILT Antigens
The following study was conducted to demonstrate the effectiveness of these recombinant HVT/ILT viruses in protecting against challenge with virulent Infectious Laryngotracheitis virus. One-day-old specific pathogen free (SPF) chicks were vaccinated with either S-HVT-051, S-HVT-052, a combination of S-HVT-051 and S-HVT-052, or a USDA-licensed, conventional vaccine comprised of ILT virus. Two to three weeks post-vaccination, vaccinated chicks, and non-vaccinated, control chicks were challenged with ILT: Birds were then observed for clinical signs of disease. The results, in Table 9, show these recombinant viruses (S-HVT-051 and S-HVT-052) gave protection against challenge with ILT virus comparable to a commercial ILT vaccine.
Animals vaccinated with the vaccines described here may be easily differentiated from animals infected with virulent ILT. This is accomplished by testing the suspect birds for antibodies to any ILT antigens other than gB or gD. Examples of such antigens are ILT glycoproteins C, E, and G. Vaccinated, uninfected birds will be negative for these antigens whereas infected birds will be positive.
TABLE 9______________________________________EFFICACY OF RECOMBINANT HVT/ILT VIRUSESAGAINST VIRULENT INFECTIOUSLARYNGOTRACHEITIS VIRUS CHALLENGEVaccine Group Dose.sup.a Protection.sup.b______________________________________S-HVT-051 2.1 .times. 10.sup.3 28/30 (93%)S-HVT-052 1.7 .times. 10.sup.3 29/29 (100%)S-HVT-051 + 2.1 .times. 10.sup.3 24/24 (100%)S-HVT-052 1.7 .times. 10.sup.3Controls 2/30 (7%)ILT.sup.c 29/30 (97%)______________________________________ .sup.a PFU/0.2 ml. .sup.b No. protected/Total; Challenge 23 weeks postvaccination. .sup.c Commercial vaccine.
Example 13
Bivalent Vaccines Against Infectious Bursal Disease and Marek's Disease
Recombinant HVT expressing proteins from IBDV make bivalent vaccines protecting against both Marek's Disease and infectious bursal disease. We have constructed several recombinant HVT expressing IBDV proteins these viruses include S-HVT-003 (example 2) and S-HVT-096.
S-HVT-096 is a recombinant herpesvirus of turkeys that contains the IBDV VP2 gene, under the control of the HCMV immediate early promoter, inserted into the short unique region. The IBDV gene is inserted in the same transcriptional orientation as the US2 gene.
S-HVT-096 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 556-60.6 with BamHI, and 602-57.F1 uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Testing of Recombinant HVT Expressing IBDV Antigens
S-HVT-096 was assayed for expression of VP2 by black plaque and western blot analysis. Both assays indicated that the virus was expressing high levels of protein which reacts specifically with an IBDV neutralizing monoclonal antibody. This virus will be useful as a vaccine against infectious bursal disease.
Example 14
Bivalent Vaccines Against Infectious Bronchitis and Marek's Disease
S-HVT-066 is a recombinant herpesvirus of turkeys that contains the MDV gB, gD and gA genes and the IBV spike and matrix genes. The IBV spike and matrix genes are under the control of the HCMV immediate early and PRV gX promoters respectively. All five genes are inserted into the short unique region. The MDV and IBV genes are inserted in the same transcriptional orientation as the US2 gene.
S-HVT-066 was constructed according to the PROCEDURE FOR GENERATING RECOMBINANT HERPESVIRUS FROM SUBGENOMIC DNA FRAGMENTS. The following combination of subgenomic clones and enzymes were used: 407-32.2C3 with NotI, 172-07.BA2 with BamHI, 407-32.5G6 with NotI, 407-32.1C1 with NotI, 437-26.24 with BamHI and HindIII, 556-60.6 with BamHI, and 567-72.1D uncut. Insertion of the appropriate DNA was confirmed by southern blot analysis.
Testing of Recombinant HVT Expressing IBV Antigens
S-HVT-066 was assayed for expression of the IBV spike protein by black plaque and western blot analysis. Both assays indicated that the virus was expressing high levels of protein which reacts specifically with an IBV neutralizing monoclonal antibody. This virus will be useful as a vaccine against infectious bronchitis.
Example 21
Vaccines utilizing HVT to express antigens from various pathogens
We also anticipate that antigens from the following pathogens may also be utilized to develop poultry vaccines: Chick anemia agent, Avian encephalomyelitis virus, Avian reovirus, Avian paramyxoviruses, Avian influenza virus, Avian adenovirus, Fowl pox virus, Avian coronavirus, Avian rotavirus, Salmonella spp E. coli, Pasteurella spp, Haemophilus spp, Chlamydia sppI Mycoplasma sppI Campylobacter spp, Bordetella spp, Poultry nematodes, cestodes, trematodes, Poultry mites/lice, Poultry protozoa (Eimeria spp, Histomonas spp, Trichomonas spp).
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50. D. R. Thomsen et al., Gene 16, 207-217, 1981.
__________________________________________________________________________# SEQUENCE LISTING- (1) GENERAL INFORMATION:- (iii) NUMBER OF SEQUENCES: 47- (2) INFORMATION FOR SEQ ID NO:1:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 3350 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 129..2522- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:- GGATACGATC GGTCTGACCC GGGGGAGTCA CCCGGGGACA GCCGTCAAGG CC - #TTGTTCCA 60- GGATAGAACT CCTCCTTCTA CAACGCTATC ATTGATGGTC AGTAGAGATC AG - #ACAAACGA 120#CAG ATT GTT CCG TTC 170AA GAT CAA ACC CAA Met Thr Asn Leu Gln A - #sp Gln Thr Gln Gln Ile Val Pro Phe# 10- ATA CGG AGC CTT CTG ATG CCA ACA ACC GGA CC - #G GCG TCC ATT CCG GAG 218Ile Arg Ser Leu Leu Met Pro Thr Thr Gly Pr - #o Ala Ser Ile Pro Glu# 30- ACA CCC TGG AGA AGC ACA CTC TCA GGT CAG AG - #A CTG ACC TAC AAT TTG 266Thr Pro Trp Arg Ser Thr Leu Ser Gly Gln Ar - #g Leu Thr Tyr Asn Leu# 45- ACT GTG GGG GAC ACA GGG TCA GGG CTA ATT GT - #C TTT TTC CCT GGA TTC 314Thr Val Gly Asp Thr Gly Ser Gly Leu Ile Va - #l Phe Phe Pro Gly Phe# 60- CCT GGC TCA ATT GTG GGT GCT CAC TAC ACA CT - #G CAG AGC AAT GGG AAC 362Pro Gly Ser Ile Val Gly Ala His Tyr Thr Le - #u Gln Ser Asn Gly Asn# 75- TAC AAG TTC GAT CGG ATG CTC CTG ACT GCC CA - #G AAC CTA CCG GCC AGT 410Tyr Lys Phe Asp Arg Met Leu Leu Thr Ala Gl - #n Asn Leu Pro Ala Ser# 90- TAC AAC TAC TGC AGG CTA GTG AGT CGG AGT CT - #C ACA GTG AGG TCA AGC 458Tyr Asn Tyr Cys Arg Leu Val Ser Arg Ser Le - #u Thr Val Arg Ser Ser#110- ACA CTT CCT GGT GGC GTT TAT GCA CTA AAC GG - #C ACC ATA AAC GCC GTG 506Thr Leu Pro Gly Gly Val Tyr Ala Leu Asn Gl - #y Thr Ile Asn Ala Val# 125- ACC TTC CAA GGA AGC CTG AGT GAA CTG ACA GA - #T GTT AGC TAC AAT GGG 554Thr Phe Gln Gly Ser Leu Ser Glu Leu Thr As - #p Val Ser Tyr Asn Gly# 140- TTG ATG TCT GCA ACA GCC AAC ATC AAC GAC AA - #A ATT GGG AAC GTC CTA 602Leu Met Ser Ala Thr Ala Asn Ile Asn Asp Ly - #s Ile Gly Asn Val Leu# 155- GTA GGG GAA GGG GTC ACC GTC CTC AGC TTA CC - #C ACA TCA TAT GAT CTT 650Val Gly Glu Gly Val Thr Val Leu Ser Leu Pr - #o Thr Ser Tyr Asp Leu# 170- GGG TAT GTG AGG CTT GGT GAC CCC ATT CCC GC - #A ATA GGG CTT GAC CCA 698Gly Tyr Val Arg Leu Gly Asp Pro Ile Pro Al - #a Ile Gly Leu Asp Pro175 1 - #80 1 - #85 1 -#90#AGG CCC AGA GTC TAC ACC 746GC AGT GACLys Met Val Ala Thr Cys Asp Ser Ser Asp Ar - #g Pro Arg Val Tyr Thr# 205- ATA ACT GCA GCC GAT GAT TAC CAA TTC TCA TC - #A CAG TAC CAA CCA GGT 794Ile Thr Ala Ala Asp Asp Tyr Gln Phe Ser Se - #r Gln Tyr Gln Pro Gly# 220- GGG GTA ACA ATC ACA CTG TTC TCA GCC AAC AT - #T GAT GCC ATC ACA AGC 842Gly Val Thr Ile Thr Leu Phe Ser Ala Asn Il - #e Asp Ala Ile Thr Ser# 235- CTC AGC GTT GGG GGA GAG CTC GTG TTT CGA AC - #A AGC GTC CAC GGC CTT 890Leu Ser Val Gly Gly Glu Leu Val Phe Arg Th - #r Ser Val His Gly Leu# 250- GTA CTG GGC GCC ACC ATC TAC CTC ATA GGC TT - #T GAT GGG ACA ACG GTA 938Val Leu Gly Ala Thr Ile Tyr Leu Ile Gly Ph - #e Asp Gly Thr Thr Val255 2 - #60 2 - #65 2 -#70- ATC ACC AGG GCT GTG GCC GCA AAC ACT GGG CT - #G ACG ACC GGC ACC GAC 986Ile Thr Arg Ala Val Ala Ala Asn Thr Gly Le - #u Thr Thr Gly Thr Asp# 285- AAC CTT ATG CCA TTC AAT CTT GTG ATT CCA AC - #A AAC GAG ATA ACC CAG1034Asn Leu Met Pro Phe Asn Leu Val Ile Pro Th - #r Asn Glu Ile Thr Gln# 300- CCA ATC ACA TCC ATC AAA CTG GAG ATA GTG AC - #C TCC AAA AGT GGT GGT1082Pro Ile Thr Ser Ile Lys Leu Glu Ile Val Th - #r Ser Lys Ser Gly Gly# 315- CAG GCA GGG GAT CAG ATG TTA TGG TCG GCA AG - #A GGG AGC CTA GCA GTG1130Gln Ala Gly Asp Gln Met Leu Trp Ser Ala Ar - #g Gly Ser Leu Ala Val# 330- ACG ATC CAT GGT GGC AAC TAT CCA GGG GCC CT - #C CGT CCC GTC ACG CTA1178Thr Ile His Gly Gly Asn Tyr Pro Gly Ala Le - #u Arg Pro Val Thr Leu335 3 - #40 3 - #45 3 -#50- GTG GCC TAC GAA AGA GTG GCA ACA GGA TCC GT - #C GTT ACG GTC GCT GGG1226Val Ala Tyr Glu Arg Val Ala Thr Gly Ser Va - #l Val Thr Val Ala Gly# 365- GTG AGC AAC TTC GAG CTG ATC CCA AAT CCT GA - #A CTA GCA AAG AAC CTG1274Val Ser Asn Phe Glu Leu Ile Pro Asn Pro Gl - #u Leu Ala Lys Asn Leu# 380- GTT ACA GAA TAC GGC CGA TTT GAC CCA GGA GC - #C ATG AAC TAC ACA AAA1322Val Thr Glu Tyr Gly Arg Phe Asp Pro Gly Al - #a Met Asn Tyr Thr Lys# 395- TTG ATA CTG AGT GAG AGG GAC CGT CTT GGC AT - #C AAG ACC GTC TGG CCA1370Leu Ile Leu Ser Glu Arg Asp Arg Leu Gly Il - #e Lys Thr Val Trp Pro# 410- ACA AGG GAG TAC ACT GAC TTT CGT GAA TAC TT - #C ATG GAG GTG GCC GAC1418Thr Arg Glu Tyr Thr Asp Phe Arg Glu Tyr Ph - #e Met Glu Val Ala Asp415 4 - #20 4 - #25 4 -#30- CTC AAC TCT CCC CTG AAG ATT GCA GGA GCA TT - #C GGC TTC AAA GAC ATA1466Leu Asn Ser Pro Leu Lys Ile Ala Gly Ala Ph - #e Gly Phe Lys Asp Ile# 445- ATC CGG GCC ATA AGG AGG ATA GCT GTG CCG GT - #G GTC TCC ACA TTG TTC1514Ile Arg Ala Ile Arg Arg Ile Ala Val Pro Va - #l Val Ser Thr Leu Phe# 460- CCA CCT GCC GCT CCC CTA GCC CAT GCA ATT GG - #G GAA GGT GTA GAC TAC1562Pro Pro Ala Ala Pro Leu Ala His Ala Ile Gl - #y Glu Gly Val Asp Tyr# 475- CTG CTG GGC GAT GAG GCA CAG GCT GCT TCA GG - #A ACT GCT CGA GCC GCG1610Leu Leu Gly Asp Glu Ala Gln Ala Ala Ser Gl - #y Thr Ala Arg Ala Ala# 490- TCA GGA AAA GCA AGA GCT GCC TCA GGC CGC AT - #A AGG CAG CTG ACT CTC1658Ser Gly Lys Ala Arg Ala Ala Ser Gly Arg Il - #e Arg Gln Leu Thr Leu495 5 - #00 5 - #05 5 -#10- GCC GCC GAC AAG GGG TAC GAG GTA GTC GCG AA - #T CTA TTC CAG GTG CCC1706Ala Ala Asp Lys Gly Tyr Glu Val Val Ala As - #n Leu Phe Gln Val Pro# 525- CAG AAT CCC GTA GTC GAC GGG ATT CTT GCT TC - #A CCT GGG GTA CTC CGC1754Gln Asn Pro Val Val Asp Gly Ile Leu Ala Se - #r Pro Gly Val Leu Arg# 540- GGT GCA CAC AAC CTC GAC TGC GTG TTA AGA GA - #G GGT GCC ACG CTA TTC1802#Glu Gly Ala Thr Leu Phe Cys Val Leu Arg# 555- CCT GTG GTT ATT ACG ACA GTG GAA GAC GCC AT - #G ACA CCC AAA GCA TTG1850Pro Val Val Ile Thr Thr Val Glu Asp Ala Me - #t Thr Pro Lys Ala Leu# 570- AAC AGC AAA ATG TTT GCT GTC ATT GAA GGC GT - #G CGA GAA GAC CTC CAA1898Asn Ser Lys Met Phe Ala Val Ile Glu Gly Va - #l Arg Glu Asp Leu Gln575 5 - #80 5 - #85 5 -#90- CCT CCA TCT CAA AGA GGA TCC TTC ATA CGA AC - #T CTC TCT GGA CAC AGA1946Pro Pro Ser Gln Arg Gly Ser Phe Ile Arg Th - #r Leu Ser Gly His Arg# 605- GTC TAT GGA TAT GCT CCA GAT GGG GTA CTT CC - #A CTG GAG ACT GGG AGA1994Val Tyr Gly Tyr Ala Pro Asp Gly Val Leu Pr - #o Leu Glu Thr Gly Arg# 620- GAC TAC ACC GTT GTC CCA ATA GAT GAT GTC TG - #G GAC GAC AGC ATT ATG2042Asp Tyr Thr Val Val Pro Ile Asp Asp Val Tr - #p Asp Asp Ser Ile Met# 635- CTG TCC AAA GAT CCC ATA CCT CCT ATT GTG GG - #A AAC AGT GGA AAT CTA2090Leu Ser Lys Asp Pro Ile Pro Pro Ile Val Gl - #y Asn Ser Gly Asn Leu# 650- GCC ATA GCT TAC ATG GAT GTG TTT CGA CCC AA - #A GTC CCA ATC CAT GTG2138Ala Ile Ala Tyr Met Asp Val Phe Arg Pro Ly - #s Val Pro Ile His Val655 6 - #60 6 - #65 6 -#70- GCT ATG ACG GGA GCC CTC AAT GCT TGT GGC GA - #G ATT GAG AAA GTA AGC2186Ala Met Thr Gly Ala Leu Asn Ala Cys Gly Gl - #u Ile Glu Lys Val Ser# 685- TTT AGA AGC ACC AAG CTC GCC ACT GCA CAC CG - #A CTT GGC CTT AAG TTG2234Phe Arg Ser Thr Lys Leu Ala Thr Ala His Ar - #g Leu Gly Leu Lys Leu# 700- GCT GGT CCC GGA GCA TTC GAT GTA AAC ACC GG - #G CCC AAC TGG GCA ACG2282Ala Gly Pro Gly Ala Phe Asp Val Asn Thr Gl - #y Pro Asn Trp Ala Thr# 715- TTC ATC AAA CGT TTC CCT CAC AAT CCA CGC GA - #C TGG GAC AGG CTC CCC2330Phe Ile Lys Arg Phe Pro His Asn Pro Arg As - #p Trp Asp Arg Leu Pro# 730- TAC CTC AAC CTA CCA TAC CTT CCA CCC AAT GC - #A GGA CGC CAG TAC CAC2378Tyr Leu Asn Leu Pro Tyr Leu Pro Pro Asn Al - #a Gly Arg Gln Tyr His735 7 - #40 7 - #45 7 -#50- CTT GCC ATG GCT GCA TCA GAG TTC AAG AGA CC - #C CGA ACT CGA GAG TGC2426Leu Ala Met Ala Ala Ser Glu Phe Lys Arg Pr - #o Arg Thr Arg Glu Cys# 765- CGT CAG AGC AAT GGA AGC AGC AGC CAA CGT GG - #A CCC ACT ATT CCA ATC2474Arg Gln Ser Asn Gly Ser Ser Ser Gln Arg Gl - #y Pro Thr Ile Pro Ile# 780- TGC ACT CAG TGT GTT CAT GTG GCT GGA AGA GA - #A TGG GAT TGT GAC TGACATG2529Cys Thr Gln Cys Val His Val Ala Gly Arg Gl - #u Trp Asp Cys Asp# 795- AACTTCGCAC TCAGCGACCC GAACGCCCAT CGGATGCGAA ATTTTTTTGC AA - #ACGACCAC2589- AAGCAGGCAG CAAGTCGCAA AGGGCCAAGT ACGGGACAGC AGGCTACGGA GT - #GGAGGCTC2649- GGGGCCCCCA CACCAGAGGA AGCACAGAGG GAAAAAGACA CACGGATCTC AA - #AGAAGATG2709- GAGACCATGG GCATCTACTT TGCAACACCA GAATGGGTAG CACTCAATGG GC - #ACCGAGGG2769- CCAAGCCCCG GCCAGCTAAA GTACGGGCAG AACACACGAG AAATACGGAC CC - #AAACGAGG2829- ACTATCTAGA CTACGTGCAT GCAGAGAAGA GCCGGTTGGC ATCAGAAGAA CA - #AATCCTAA2889- GGGCAGCTAC GTCAGATCTA CGGGGCTCCA GGACAGGCAG AGCACCCCAA GC - #TTTCATAG2949- ACGAAGTTGC CAAAGTCTAT GAAATCAACC ATGGACGTGG CCCAAACCAA GA - #ACAGATGA3009- AAGATCTGCT CTTGACTGCG ATGGAGATGA AGCATCGCAA TCCCAGGCGG GC - #TCTACCAA3069- AGCCCAAGCC AAAACCCAAT GCTCCAACAC AGAGACCCCC TGGTCGGCTG GG - #GCTGGATC3129- AGGACCGTCT CTGATGAGGA CCTTGAGTGA GGCTCCTGGG AGTCTCCCGA CA - #ACACCCGC3189- GCAGGTGTGG ACACAATTCG GCCTTACAAC ATCCCAAATT GGATCCGTTC GC - #GGGTCCCC3249- AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA - #AAAGTACC3309# 3350 ACCA GCCGGATCCC TCGAGGGATC C- (2) INFORMATION FOR SEQ ID NO:2:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 797 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:- Met Thr Asn Leu Gln Asp Gln Thr Gln Gln Il - #e Val Pro Phe Ile Arg# 15- Ser Leu Leu Met Pro Thr Thr Gly Pro Ala Se - #r Ile Pro Glu Thr Pro# 30- Trp Arg Ser Thr Leu Ser Gly Gln Arg Leu Th - #r Tyr Asn Leu Thr Val# 45- Gly Asp Thr Gly Ser Gly Leu Ile Val Phe Ph - #e Pro Gly Phe Pro Gly# 60- Ser Ile Val Gly Ala His Tyr Thr Leu Gln Se - #r Asn Gly Asn Tyr Lys# 80- Phe Asp Arg Met Leu Leu Thr Ala Gln Asn Le - #u Pro Ala Ser Tyr Asn# 95- Tyr Cys Arg Leu Val Ser Arg Ser Leu Thr Va - #l Arg Ser Ser Thr Leu# 110- Pro Gly Gly Val Tyr Ala Leu Asn Gly Thr Il - #e Asn Ala Val Thr Phe# 125- Gln Gly Ser Leu Ser Glu Leu Thr Asp Val Se - #r Tyr Asn Gly Leu Met# 140- Ser Ala Thr Ala Asn Ile Asn Asp Lys Ile Gl - #y Asn Val Leu Val Gly145 1 - #50 1 - #55 1 -#60- Glu Gly Val Thr Val Leu Ser Leu Pro Thr Se - #r Tyr Asp Leu Gly Tyr# 175- Val Arg Leu Gly Asp Pro Ile Pro Ala Ile Gl - #y Leu Asp Pro Lys Met# 190- Val Ala Thr Cys Asp Ser Ser Asp Arg Pro Ar - #g Val Tyr Thr Ile Thr# 205- Ala Ala Asp Asp Tyr Gln Phe Ser Ser Gln Ty - #r Gln Pro Gly Gly Val# 220#Ala Ile Thr Ser Leu Serer Ala Asn Ile Asp225 2 - #30 2 - #35 2 -#40- Val Gly Gly Glu Leu Val Phe Arg Thr Ser Va - #l His Gly Leu Val Leu# 255- Gly Ala Thr Ile Tyr Leu Ile Gly Phe Asp Gl - #y Thr Thr Val Ile Thr# 270- Arg Ala Val Ala Ala Asn Thr Gly Leu Thr Th - #r Gly Thr Asp Asn Leu# 285- Met Pro Phe Asn Leu Val Ile Pro Thr Asn Gl - #u Ile Thr Gln Pro Ile# 300- Thr Ser Ile Lys Leu Glu Ile Val Thr Ser Ly - #s Ser Gly Gly Gln Ala305 3 - #10 3 - #15 3 -#20- Gly Asp Gln Met Leu Trp Ser Ala Arg Gly Se - #r Leu Ala Val Thr Ile# 335- His Gly Gly Asn Tyr Pro Gly Ala Leu Arg Pr - #o Val Thr Leu Val Ala# 350- Tyr Glu Arg Val Ala Thr Gly Ser Val Val Th - #r Val Ala Gly Val Ser# 365- Asn Phe Glu Leu Ile Pro Asn Pro Glu Leu Al - #a Lys Asn Leu Val Thr# 380- Glu Tyr Gly Arg Phe Asp Pro Gly Ala Met As - #n Tyr Thr Lys Leu Ile385 3 - #90 3 - #95 4 -#00- Leu Ser Glu Arg Asp Arg Leu Gly Ile Lys Th - #r Val Trp Pro Thr Arg# 415- Glu Tyr Thr Asp Phe Arg Glu Tyr Phe Met Gl - #u Val Ala Asp Leu Asn# 430- Ser Pro Leu Lys Ile Ala Gly Ala Phe Gly Ph - #e Lys Asp Ile Ile Arg# 445- Ala Ile Arg Arg Ile Ala Val Pro Val Val Se - #r Thr Leu Phe Pro Pro# 460- Ala Ala Pro Leu Ala His Ala Ile Gly Glu Gl - #y Val Asp Tyr Leu Leu465 4 - #70 4 - #75 4 -#80- Gly Asp Glu Ala Gln Ala Ala Ser Gly Thr Al - #a Arg Ala Ala Ser Gly# 495#Gln Leu Thr Leu Ala Alaer Gly Arg Ile Arg# 510- Asp Lys Gly Tyr Glu Val Val Ala Asn Leu Ph - #e Gln Val Pro Gln Asn# 525- Pro Val Val Asp Gly Ile Leu Ala Ser Pro Gl - #y Val Leu Arg Gly Ala# 540- His Asn Leu Asp Cys Val Leu Arg Glu Gly Al - #a Thr Leu Phe Pro Val545 5 - #50 5 - #55 5 -#60- Val Ile Thr Thr Val Glu Asp Ala Met Thr Pr - #o Lys Ala Leu Asn Ser# 575- Lys Met Phe Ala Val Ile Glu Gly Val Arg Gl - #u Asp Leu Gln Pro Pro# 590- Ser Gln Arg Gly Ser Phe Ile Arg Thr Leu Se - #r Gly His Arg Val Tyr# 605- Gly Tyr Ala Pro Asp Gly Val Leu Pro Leu Gl - #u Thr Gly Arg Asp Tyr# 620- Thr Val Val Pro Ile Asp Asp Val Trp Asp As - #p Ser Ile Met Leu Ser625 6 - #30 6 - #35 6 -#40- Lys Asp Pro Ile Pro Pro Ile Val Gly Asn Se - #r Gly Asn Leu Ala Ile# 655- Ala Tyr Met Asp Val Phe Arg Pro Lys Val Pr - #o Ile His Val Ala Met# 670- Thr Gly Ala Leu Asn Ala Cys Gly Glu Ile Gl - #u Lys Val Ser Phe Arg# 685- Ser Thr Lys Leu Ala Thr Ala His Arg Leu Gl - #y Leu Lys Leu Ala Gly# 700- Pro Gly Ala Phe Asp Val Asn Thr Gly Pro As - #n Trp Ala Thr Phe Ile705 7 - #10 7 - #15 7 -#20- Lys Arg Phe Pro His Asn Pro Arg Asp Trp As - #p Arg Leu Pro Tyr Leu# 735- Asn Leu Pro Tyr Leu Pro Pro Asn Ala Gly Ar - #g Gln Tyr His Leu Ala# 750- Met Ala Ala Ser Glu Phe Lys Arg Pro Arg Th - #r Arg Glu Cys Arg Gln# 765#Thr Ile Pro Ile Cys Threr Gln Arg Gly Pro# 780- Gln Cys Val His Val Ala Gly Arg Glu Trp As - #p Cys Asp785 7 - #90 7 - #95- (2) INFORMATION FOR SEQ ID NO:3:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 5426 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 73..1182#/product= "HVT UL42"NFORMATION:- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1306..2574#/product= "HVT UL43"NFORMATION:- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 2790..4259#/product= "HVT gA" INFORMATION:- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 4701..5339#/product= "HVT UL45"NFORMATION:- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:- GGATCCGAGC TTCTACTATA CAACGCGGAC GATAATTTTG TCCACCCCAT CG - #GTGTTCGA 60- GAAAGGGTTT TT ATG ATG GCA GGA ATA ACT GTC GCA - # TGT GAC CAC ACT 108 Met Met Al - #a Gly Ile Thr Val Ala Cys Asp His Thr# 10- GCA GGA GAG GCT CAT ACA CCC GAG GAT ATG CA - #A AAG AAA TGG AGG ATT 156Ala Gly Glu Ala His Thr Pro Glu Asp Met Gl - #n Lys Lys Trp Arg Ile# 25- ATA TTG GCA GGG GAA AAA TTC ATG ACT ATA TC - #G GCA TCG TTG AAA TCG 204Ile Leu Ala Gly Glu Lys Phe Met Thr Ile Se - #r Ala Ser Leu Lys Ser# 40- ATC GTC AGT TGT GTG AAA AAC CCC CTT CTC AC - #G TTT GGC GCA GAT GGG 252Ile Val Ser Cys Val Lys Asn Pro Leu Leu Th - #r Phe Gly Ala Asp Gly# 60- CTC ATT GTA CAA GGT ACT GTC TGC GGA CAG CG - #C ATT TTT GTT CCA ATC 300Leu Ile Val Gln Gly Thr Val Cys Gly Gln Ar - #g Ile Phe Val Pro Ile# 75- GAC CGT GAT TCC TTC AGC GAA TAT GAA TGG CA - #T GGG CCA ACT GCG ATG 348Asp Arg Asp Ser Phe Ser Glu Tyr Glu Trp Hi - #s Gly Pro Thr Ala Met# 90- TTT CTA GCA TTA ACT GAT TCC AGA CGC ACT CT - #T TTA GAT GCA TTC AAA 396Phe Leu Ala Leu Thr Asp Ser Arg Arg Thr Le - #u Leu Asp Ala Phe Lys# 105- TGT GAA AAG AGA AGG GCA ATT GAC GTC TCC TT - #T ACC TTC GCG GGA GAG 444Cys Glu Lys Arg Arg Ala Ile Asp Val Ser Ph - #e Thr Phe Ala Gly Glu# 120#ACA TAC ATG ACC GAC GGT 492AA GCC GTCPro Pro Cys Arg His Leu Ile Gln Ala Val Th - #r Tyr Met Thr Asp Gly125 1 - #30 1 - #35 1 -#40- GGT TCA GTA TCG AAT ACA ATC ATT AAA TAT GA - #G CTC TGG AAT GCG TCT 540Gly Ser Val Ser Asn Thr Ile Ile Lys Tyr Gl - #u Leu Trp Asn Ala Ser# 155- ACA ATT TTC CCC CAA AAA ACT CCC GAT GTT AC - #C TTT TCT CTA AAC AAA 588Thr Ile Phe Pro Gln Lys Thr Pro Asp Val Th - #r Phe Ser Leu Asn Lys# 170- CAA CAA TTG AAC AAA ATA TTG GCC GTC GCT TC - #A AAA CTG CAA CAC GAA 636Gln Gln Leu Asn Lys Ile Leu Ala Val Ala Se - #r Lys Leu Gln His Glu# 185- GAA CTT GTA TTC TCT TTA AAA CCT GAA GGA GG - #G TTC TAC GTA GGA ACG 684Glu Leu Val Phe Ser Leu Lys Pro Glu Gly Gl - #y Phe Tyr Val Gly Thr# 200- GTT TGT ACT GTT ATA AGT TTC GAA GTA GAT GG - #G ACT GCC ATG ACT CAG 732Val Cys Thr Val Ile Ser Phe Glu Val Asp Gl - #y Thr Ala Met Thr Gln205 2 - #10 2 - #15 2 -#20- TAT CCT TAC AAC CCT CCA ACC TCG GCT ACC CT - #A GCT CTC GTA GTA GCA 780Tyr Pro Tyr Asn Pro Pro Thr Ser Ala Thr Le - #u Ala Leu Val Val Ala# 235- TGC AGA AAG AAG AAG GCG AAT AAA AAC ACT AT - #T TTA ACG GCC TAT GGA 828Cys Arg Lys Lys Lys Ala Asn Lys Asn Thr Il - #e Leu Thr Ala Tyr Gly# 250- AGT GGT AAA CCC TTT TGT GTT GCA TTG GAA GA - #T ACT AGT GCA TTT AGA 876Ser Gly Lys Pro Phe Cys Val Ala Leu Glu As - #p Thr Ser Ala Phe Arg# 265- AAT ATC GTC AAT AAA ATC AAG GCG GGT ACG TC - #G GGA GTT GAT CTG GGG 924Asn Ile Val Asn Lys Ile Lys Ala Gly Thr Se - #r Gly Val Asp Leu Gly# 280- TTT TAT ACA ACT TGC GAT CCG CCG ATG CTA TG - #T ATT CGC CCA CAC GCA 972Phe Tyr Thr Thr Cys Asp Pro Pro Met Leu Cy - #s Ile Arg Pro His Ala285 2 - #90 2 - #95 3 -#00- TTT GGA AGT CCT ACC GCA TTC CTG TTT TGT AA - #C ACA GAC TGT ATG ACA1020Phe Gly Ser Pro Thr Ala Phe Leu Phe Cys As - #n Thr Asp Cys Met Thr# 315- ATA TAT GAA CTG GAA GAA GTA AGC GCC GTT GA - #T GGT GCA ATC CGA GCA1068Ile Tyr Glu Leu Glu Glu Val Ser Ala Val As - #p Gly Ala Ile Arg Ala# 330- AAA CGC ATC AAC GAA TAT TTC CCA ACA GTA TC - #G CAG GCT ACT TCC AAG1116Lys Arg Ile Asn Glu Tyr Phe Pro Thr Val Se - #r Gln Ala Thr Ser Lys# 345- AAG AGA AAA CAG TCG CCG CCC CCT ATC GAA AG - #A GAA AGG AAA ACC ACC1164Lys Arg Lys Gln Ser Pro Pro Pro Ile Glu Ar - #g Glu Arg Lys Thr Thr# 360- AGA GCG GAT ACC CAA TAAAATGCCA GACAAACCCG GCATCCTGG - #T TAGAGGGCAG1219Arg Ala Asp Thr Gln365 3 - #70- GTGGGCTGGG CCAACCTTCA CGGGCGTCCG ACAGATCGGT GACACTCATA CG - #TTAACTAA1279#AGC TCG AGT CCT 1332GAAAAT ATG CCT TCC GGA GCC#Ala Ser Ser Ser ProSer Gly# 5 1- CCA CCA GCT TAT ACA TCT GCA GCT CCG CTT GA - #G ACT TAT AAC AGC TGG1380Pro Pro Ala Tyr Thr Ser Ala Ala Pro Leu Gl - #u Thr Tyr Asn Ser Trp# 25- CTA AGT GCC TTT TCA TGC GCA TAT CCC CAA TG - #C ACT GCG GGA AGA GGA1428Leu Ser Ala Phe Ser Cys Ala Tyr Pro Gln Cy - #s Thr Ala Gly Arg Gly# 40- CAT CGA CAA AAT GGC AAG AAG TGT ATA CGG TG - #T ATA GTG ATC AGT GTA1476His Arg Gln Asn Gly Lys Lys Cys Ile Arg Cy - #s Ile Val Ile Ser Val# 55- TGT TCC TTA GTG TGC ATC GCT GCA CAT TTA GC - #T GTT ACC GTG TCG GGA1524Cys Ser Leu Val Cys Ile Ala Ala His Leu Al - #a Val Thr Val Ser Gly# 70- GTG GCA TTA ATT CCG CTT ATC GAT CAA AAC AG - #A GCT TAC GGA AAC TGT1572Val Ala Leu Ile Pro Leu Ile Asp Gln Asn Ar - #g Ala Tyr Gly Asn Cys# 85- ACG GTA TGT GTA ATT GCC GGA TTC ATC GCT AC - #G TTT GCT GCA CGA CTT1620Thr Val Cys Val Ile Ala Gly Phe Ile Ala Th - #r Phe Ala Ala Arg Leu#105- ACG ATA AGA CTT TCG GAA ACG CTT ATG CTA GT - #G GGC AAG CCG GCG CAG1668Thr Ile Arg Leu Ser Glu Thr Leu Met Leu Va - #l Gly Lys Pro Ala Gln# 120- TTT ATA TTT GCT ATA ATC GCT TCC GTT GCG GA - #A ACA CTG ATC AAT AAC1716Phe Ile Phe Ala Ile Ile Ala Ser Val Ala Gl - #u Thr Leu Ile Asn Asn# 135- GAG GCG CTT GCC ATC AGT AAT ACT ACT TAC AA - #A ACT GCA TTG CGA ATA1764Glu Ala Leu Ala Ile Ser Asn Thr Thr Tyr Ly - #s Thr Ala Leu Arg Ile# 150- ATC GAA GTA ACA TCT TTG GCG TGT TTT GTT AT - #G CTC GGG GCA ATA ATT1812Ile Glu Val Thr Ser Leu Ala Cys Phe Val Me - #t Leu Gly Ala Ile Ile# 165- ACA TCC CAC AAC TAT GTC TGC ATT TCA ACG GC - #A GGG GAC TTG ACT TGG1860Thr Ser His Asn Tyr Val Cys Ile Ser Thr Al - #a Gly Asp Leu Thr Trp170 1 - #75 1 - #80 1 -#85- AAG GGC GGG ATT TTT CAT GCT TAC CAC GGA AC - #A TTA CTC GGT ATA ACA1908Lys Gly Gly Ile Phe His Ala Tyr His Gly Th - #r Leu Leu Gly Ile Thr# 200- ATA CCA AAC ATA CAC CCA ATC CCT CTC GCG GG - #G TTT CTT GCA GTC TAT1956#Gly Phe Leu Ala Val Tyr Ile Pro Leu Ala# 215- ACA ATA TTG GCT ATA AAT ATC GCT AGA GAT GC - #A AGC GCT ACA TTA TTA2004Thr Ile Leu Ala Ile Asn Ile Ala Arg Asp Al - #a Ser Ala Thr Leu Leu# 230- TCC ACT TGC TAT TAT CGC AAT TGC CGC GAG AG - #G ACT ATA CTT CGC CCT2052Ser Thr Cys Tyr Tyr Arg Asn Cys Arg Glu Ar - #g Thr Ile Leu Arg Pro# 245- TCT CGT CTC GGA CAT GGT TAC ACA ATC CCT TC - #T CCC GGT GCC GAT ATG2100Ser Arg Leu Gly His Gly Tyr Thr Ile Pro Se - #r Pro Gly Ala Asp Met250 2 - #55 2 - #60 2 -#65- CTT TAT GAA GAA GAC GTA TAT AGT TTT GAC GC - #A GCT AAA GGC CAT TAT2148Leu Tyr Glu Glu Asp Val Tyr Ser Phe Asp Al - #a Ala Lys Gly His Tyr# 280- TCG TCA ATA TTT CTA TGT TAT GCC ATG GGG CT - #T ACA ACA CCG CTG ATT2196Ser Ser Ile Phe Leu Cys Tyr Ala Met Gly Le - #u Thr Thr Pro Leu Ile# 295- ATT GCG CTC CAT AAA TAT ATG GCG GGC ATT AA - #A AAT TCG TCA GAT TGG2244Ile Ala Leu His Lys Tyr Met Ala Gly Ile Ly - #s Asn Ser Ser Asp Trp# 310- ACT GCT ACA TTA CAA GGC ATG TAC GGG CTT GT - #C TTG GGA TCG CTA TCG2292Thr Ala Thr Leu Gln Gly Met Tyr Gly Leu Va - #l Leu Gly Ser Leu Ser# 325- TCA CTA TGT ATT CCA TCC AGC AAC AAC GAT GC - #C CTA ATT CGT CCC ATT2340Ser Leu Cys Ile Pro Ser Ser Asn Asn Asp Al - #a Leu Ile Arg Pro Ile330 3 - #35 3 - #40 3 -#45- CAA ATT TTG ATA TTG ATA ATC GGT GCA CTG GC - #C ATT GCA TTG GCT GGA2388Gln Ile Leu Ile Leu Ile Ile Gly Ala Leu Al - #a Ile Ala Leu Ala Gly# 360- TGT GGT CAA ATT ATA GGG CCT ACA TTA TTT GC - #C GCG AGT TCG GCT GCG2436Cys Gly Gln Ile Ile Gly Pro Thr Leu Phe Al - #a Ala Ser Ser Ala Ala# 375- ATG TCA TGT TTT ACA TGT ATC AAT ATT CGC GC - #T ACT AAT AAG GGT GTC2484Met Ser Cys Phe Thr Cys Ile Asn Ile Arg Al - #a Thr Asn Lys Gly Val# 390- AAC AAA TTG GCA GCA GCC AGT GTC GTG AAA TC - #T GTA CTG GGC TTC ATT2532Asn Lys Leu Ala Ala Ala Ser Val Val Lys Se - #r Val Leu Gly Phe Ile# 405- ATT TCC GGG ATG CTT ACT TGC GTG CTA TTA CC - #A CTA TCG TGATAGATCG2581Ile Ser Gly Met Leu Thr Cys Val Leu Leu Pr - #o Leu Ser410 4 - #15 4 - #20- TCGGTCTGCG CATCGCCCAT GCTGGCGGAA CGCTCTTTCG AACCGTGAAT AA - #AACTTTGT2641- ATCTACTAAA CAATAACTTT GTGTTTTATT GAGCGGTCGA AAACAATGAG GA - #GCTGCAAT2701- TTAAAGCTAA CCGCATACGC CGGGCGGGTA AAGACCATTT TATACCATAT TA - #CGCATCTA2761#CGC GTT CTA 2813C GCAAGTAT ATG GTT TCC AAC ATG#Asn Met Arg Val Leul Ser# 5 1- CGC GTA CTG CGC CTG ACG GGA TGG GTG GGC AT - #A TTT CTA GTT CTG TCT2861Arg Val Leu Arg Leu Thr Gly Trp Val Gly Il - #e Phe Leu Val Leu Ser# 20- TTA CAG CAA ACC TCT TGT GCC GGA TTG CCC CA - #T AAC GTC GAT ACC CAT2909Leu Gln Gln Thr Ser Cys Ala Gly Leu Pro Hi - #s Asn Val Asp Thr His# 40- CAT ATC CTA ACT TTC AAC CCT TCT CCC ATT TC - #G GCC GAT GGC GTT CCT2957His Ile Leu Thr Phe Asn Pro Ser Pro Ile Se - #r Ala Asp Gly Val Pro# 55- TTG TCA GAG GTG CCC AAT TCG CCT ACG ACC GA - #A TTA TCT ACA ACT GTC3005Leu Ser Glu Val Pro Asn Ser Pro Thr Thr Gl - #u Leu Ser Thr Thr Val# 70- GCC ACC AAG ACA GCT GTA CCG ACG ACT GAA AG - #C ACT AGT TCC TCC GAA3053Ala Thr Lys Thr Ala Val Pro Thr Thr Glu Se - #r Thr Ser Ser Ser Glu# 85- GCG CAC CGC AAC TCT TCT CAC AAA ATA CCT GA - #T ATA ATC TGC GAC CGA3101Ala His Arg Asn Ser Ser His Lys Ile Pro As - #p Ile Ile Cys Asp Arg# 100- GAA GAA GTA TTC GTA TTC CTT AAC AAT ACA GG - #A AGA ATT TTG TGT GAC3149Glu Glu Val Phe Val Phe Leu Asn Asn Thr Gl - #y Arg Ile Leu Cys Asp105 1 - #10 1 - #15 1 -#20- CTT ATA GTC GAC CCC CCT TCA GAC GAT GAA TG - #G TCC AAC TTC GCT CTT3197Leu Ile Val Asp Pro Pro Ser Asp Asp Glu Tr - #p Ser Asn Phe Ala Leu# 135- GAC GTC ACG TTC AAT CCA ATC GAA TAC CAC GC - #C AAC GAA AAG AAT GTA3245Asp Val Thr Phe Asn Pro Ile Glu Tyr His Al - #a Asn Glu Lys Asn Val# 150- GAG GTT GCC CGA GTG GCC GGT CTA TAC GGA GT - #A CCG GGG TCG GAT TAT3293Glu Val Ala Arg Val Ala Gly Leu Tyr Gly Va - #l Pro Gly Ser Asp Tyr# 165- GCA TAC CCT AGG AAA TCG GAA TTA ATA TCC TC - #C ATT CGA CGG GAT CCC3341Ala Tyr Pro Arg Lys Ser Glu Leu Ile Ser Se - #r Ile Arg Arg Asp Pro# 180- CAG GGT TCT TTC TGG ACT AGT CCT ACA CCC CG - #T GGA AAT AAA TAT TTC3389Gln Gly Ser Phe Trp Thr Ser Pro Thr Pro Ar - #g Gly Asn Lys Tyr Phe185 1 - #90 1 - #95 2 -#00- ATA TGG ATT AAT AAA ACA ATG CAC ACC ATG GG - #C GTG GAA GTT AGA AAT3437Ile Trp Ile Asn Lys Thr Met His Thr Met Gl - #y Val Glu Val Arg Asn# 215- GTC GAC TAC AAA GAC AAC GGC TAC TTT CAA GT - #G ATA CTG CGT GAT AGA3485Val Asp Tyr Lys Asp Asn Gly Tyr Phe Gln Va - #l Ile Leu Arg Asp Arg# 230- TTT AAT CGC CCA TTG GTA GAA AAA CAT ATT TA - #C ATG CGT GTG TGC CAA3533Phe Asn Arg Pro Leu Val Glu Lys His Ile Ty - #r Met Arg Val Cys Gln# 245- CGA CCC GCA TCC GTG GAT GTA TTG GCC CCT CC - #A GTT CTC AGC GGA GAA3581Arg Pro Ala Ser Val Asp Val Leu Ala Pro Pr - #o Val Leu Ser Gly Glu# 260- AAC TAC AAA GCA TCT TGC ATC GTT AGA CAT TT - #T TAT CCC CCG GGA TCT3629Asn Tyr Lys Ala Ser Cys Ile Val Arg His Ph - #e Tyr Pro Pro Gly Ser265 2 - #70 2 - #75 2 -#80- GTC TAC GTA TCT TGG AGA CGT AAC GGA AAC AT - #T GCC ACA CCC CGC AAG3677Val Tyr Val Ser Trp Arg Arg Asn Gly Asn Il - #e Ala Thr Pro Arg Lys# 295- GAC CGT GAC GGG AGT TTT TGG TGG TTC GAA TC - #T GGC CGC GGG GCC ACA3725Asp Arg Asp Gly Ser Phe Trp Trp Phe Glu Se - #r Gly Arg Gly Ala Thr# 310- CTA GTA TCC ACA ATA ACC CTC GGA AAC TCT GG - #A CTC GAA TCT CCT CCA3773Leu Val Ser Thr Ile Thr Leu Gly Asn Ser Gl - #y Leu Glu Ser Pro Pro# 325- AAG GTT TCC TGC TTG GTA GCG TGG AGG CAA GG - #C GAT ATG ATA AGC ACA3821Lys Val Ser Cys Leu Val Ala Trp Arg Gln Gl - #y Asp Met Ile Ser Thr# 340- TCG AAT GCT ACA GCT GTA CCG ACG GTA TAT TA - #T CAC CCC CGT ATC TCT3869#Tyr His Pro Arg Ile Ser Pro Thr Val Tyr345 3 - #50 3 - #55 3 -#60- CTG GCA TTT AAA GAT GGG TAT GCA ATA TGT AC - #T ATA GAA TGT GTT CCC3917Leu Ala Phe Lys Asp Gly Tyr Ala Ile Cys Th - #r Ile Glu Cys Val Pro# 375- TCT GGG ATT ACT GTG AGG TGG TTA GTT CAT GA - #T GAA CCC CAG CCT AAC3965Ser Gly Ile Thr Val Arg Trp Leu Val His As - #p Glu Pro Gln Pro Asn# 390- ACA ACT TAT GAT ACT GTG GTT ACA GGT CTC TG - #C AGG ACC ATC GAT CGT4013Thr Thr Tyr Asp Thr Val Val Thr Gly Leu Cy - #s Arg Thr Ile Asp Arg# 405- TAT AGA AAT CTC GCC AGT CGG ATT CCA GTC CA - #G GAC AAC TGG GCG AAA4061Tyr Arg Asn Leu Ala Ser Arg Ile Pro Val Gl - #n Asp Asn Trp Ala Lys# 420- ACG AAG TAT ACG TGC AGA CTA ATT GGA TAT CC - #G TTC GAC GTG GAT AGA4109Thr Lys Tyr Thr Cys Arg Leu Ile Gly Tyr Pr - #o Phe Asp Val Asp Arg425 4 - #30 4 - #35 4 -#40- TTT CAA AAT TCC GAA TAT TAT GAT GCA ACG CC - #G TCG GCA AGA GGA ATG4157Phe Gln Asn Ser Glu Tyr Tyr Asp Ala Thr Pr - #o Ser Ala Arg Gly Met# 455- CCG ATG ATT GTA ACA ATT ACG GCC GTT CTA GG - #A CTG GCC TTG TTT TTA4205Pro Met Ile Val Thr Ile Thr Ala Val Leu Gl - #y Leu Ala Leu Phe Leu# 470- GGT ATT GGT ATC ATT ATC ACA GCC CTA TGC TT - #T TAC CTA CCG GGG CGG4253Gly Ile Gly Ile Ile Ile Thr Ala Leu Cys Ph - #e Tyr Leu Pro Gly Arg# 485- AAT TAAGATTAAC CATCGTATGT GATATAAAAA TTATTAAGTG TTATAACCG - #A4306Asn 490- TCGCATTCTT CTGTTTCGAT TCACAATAAA TAAAATGGTA TTGTAATCAG CA - #CCATCGCA4366- TTGTTTCGTA GATGACTCAT GTTCAGTCCG CGTGATGTCA AAAATACGTA TT - #TTTGGTAT4426- CACGCAGCGG CCAAAATGCC CATTATGTTA TTTTTACTCC AAACGCGGTA TT - #TAAAACAT4486- CGGGACGTAC ATCATGTGGC GCACGTTAAT CGTATACGGT GCCGCTACAT TA - #AAAATCGC4546- AAGTCTCCGA ATATCAAGCT CACGGCCAAA ACGTCGGTAA TAATCTTACG CA - #TCGAATGT4606- GATACGGATA CCGTACAATC GCTGAGTAGA TTTCCTATAT AGTTACTCAG TA - #GTGATACA4666- CAATCACAAA ATCGCTGGGG TATATCATAT AAGA ATG ATG TCG C - #CC ACC CCT4718# Met Met Ser Pro Thr Pro# 5 1- GAA GAT GAT CGC GAT CTC GTT GTG GTT CGT GG - #A CGT CTC CGA ATG ATG4766Glu Asp Asp Arg Asp Leu Val Val Val Arg Gl - #y Arg Leu Arg Met Met# 20- GAT AGC GGC ACG GAA ACA GAT AGA GAG CAA CG - #A CAT CCA CGT ACG ACT4814Asp Ser Gly Thr Glu Thr Asp Arg Glu Gln Ar - #g His Pro Arg Thr Thr# 35#GGA ATG GTA TTT ACC ATA 4862GT ACG ATATrp Arg Ser Ile Cys Cys Gly Cys Thr Ile Gl - #y Met Val Phe Thr Ile# 50- TTC GTT CTC GTA GCG GCA GTA TTG TTG GGA TC - #A CTA TTC ACT GTT TCA4910Phe Val Leu Val Ala Ala Val Leu Leu Gly Se - #r Leu Phe Thr Val Ser# 70- TAC ATG GCC ATG GAA TCG GGA ACA TGT CCC GA - #T GAA TGG ATT GGT TTG4958Tyr Met Ala Met Glu Ser Gly Thr Cys Pro As - #p Glu Trp Ile Gly Leu# 85- GGT TAT AGT TGC ATG CGC GTG GCC GGG AAA AA - #T GCA ACT GAT CTT GAG5006Gly Tyr Ser Cys Met Arg Val Ala Gly Lys As - #n Ala Thr Asp Leu Glu# 100- GCG TTG GAT ACA TGT GCT CGG CAT AAC AGC AA - #A CTT ATT GAC TTC GCA5054Ala Leu Asp Thr Cys Ala Arg His Asn Ser Ly - #s Leu Ile Asp Phe Ala# 115- AAC GCC AAA GTT CTG GTT GAA GCT ATC GCC CC - #A TTC GGT GTG CCA AAT5102Asn Ala Lys Val Leu Val Glu Ala Ile Ala Pr - #o Phe Gly Val Pro Asn# 130- GCA GCA TAT GGG GAA GTC TTC CGG TTA AGG GA - #C AGC AAA ACC ACG TGT5150Ala Ala Tyr Gly Glu Val Phe Arg Leu Arg As - #p Ser Lys Thr Thr Cys135 1 - #40 1 - #45 1 -#50- ATA CGA CCT ACC ATG GGA GGA CCC GTG TCG GC - #A GAC TGT CCT GTA ACA5198Ile Arg Pro Thr Met Gly Gly Pro Val Ser Al - #a Asp Cys Pro Val Thr# 165- TGT ACC GTT ATA TGT CAG CGA CCC AGG CCT CT - #A AGT ACC ATG TCT TCC5246Cys Thr Val Ile Cys Gln Arg Pro Arg Pro Le - #u Ser Thr Met Ser Ser# 180- ATC ATT AGA GAT GCC CGC GTG TAT CTT CAT TT - #A GAA CGA CGC GAT TAT5294Ile Ile Arg Asp Ala Arg Val Tyr Leu His Le - #u Glu Arg Arg Asp Tyr# 195- TAT GAA GTC TAC GCC TCT GTC CTC TCT AAT GC - #G ATG AGT AAA TAAAAACGCA5346Tyr Glu Val Tyr Ala Ser Val Leu Ser Asn Al - #a Met Ser Lys# 210- CCTCTAACGG TTACTGTGTT TATTATCCAA TCACACCATA GACATTATTA CA - #ATAATATG5406# 542 - #6- (2) INFORMATION FOR SEQ ID NO:4:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 369 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:- Met Met Ala Gly Ile Thr Val Ala Cys Asp Hi - #s Thr Ala Gly Glu Ala# 15- His Thr Pro Glu Asp Met Gln Lys Lys Trp Ar - #g Ile Ile Leu Ala Gly# 30- Glu Lys Phe Met Thr Ile Ser Ala Ser Leu Ly - #s Ser Ile Val Ser Cys# 45- Val Lys Asn Pro Leu Leu Thr Phe Gly Ala As - #p Gly Leu Ile Val Gln# 60- Gly Thr Val Cys Gly Gln Arg Ile Phe Val Pr - #o Ile Asp Arg Asp Ser# 80- Phe Ser Glu Tyr Glu Trp His Gly Pro Thr Al - #a Met Phe Leu Ala Leu# 95- Thr Asp Ser Arg Arg Thr Leu Leu Asp Ala Ph - #e Lys Cys Glu Lys Arg# 110- Arg Ala Ile Asp Val Ser Phe Thr Phe Ala Gl - #y Glu Pro Pro Cys Arg# 125- His Leu Ile Gln Ala Val Thr Tyr Met Thr As - #p Gly Gly Ser Val Ser# 140- Asn Thr Ile Ile Lys Tyr Glu Leu Trp Asn Al - #a Ser Thr Ile Phe Pro145 1 - #50 1 - #55 1 -#60- Gln Lys Thr Pro Asp Val Thr Phe Ser Leu As - #n Lys Gln Gln Leu Asn# 175- Lys Ile Leu Ala Val Ala Ser Lys Leu Gln Hi - #s Glu Glu Leu Val Phe# 190- Ser Leu Lys Pro Glu Gly Gly Phe Tyr Val Gl - #y Thr Val Cys Thr Val# 205- Ile Ser Phe Glu Val Asp Gly Thr Ala Met Th - #r Gln Tyr Pro Tyr Asn# 220- Pro Pro Thr Ser Ala Thr Leu Ala Leu Val Va - #l Ala Cys Arg Lys Lys225 2 - #30 2 - #35 2 -#40- Lys Ala Asn Lys Asn Thr Ile Leu Thr Ala Ty - #r Gly Ser Gly Lys Pro# 255- Phe Cys Val Ala Leu Glu Asp Thr Ser Ala Ph - #e Arg Asn Ile Val Asn# 270- Lys Ile Lys Ala Gly Thr Ser Gly Val Asp Le - #u Gly Phe Tyr Thr Thr# 285- Cys Asp Pro Pro Met Leu Cys Ile Arg Pro Hi - #s Ala Phe Gly Ser Pro# 300- Thr Ala Phe Leu Phe Cys Asn Thr Asp Cys Me - #t Thr Ile Tyr Glu Leu305 3 - #10 3 - #15 3 -#20- Glu Glu Val Ser Ala Val Asp Gly Ala Ile Ar - #g Ala Lys Arg Ile Asn# 335- Glu Tyr Phe Pro Thr Val Ser Gln Ala Thr Se - #r Lys Lys Arg Lys Gln# 350- Ser Pro Pro Pro Ile Glu Arg Glu Arg Lys Th - #r Thr Arg Ala Asp Thr# 365- Gln- (2) INFORMATION FOR SEQ ID NO:5:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 422 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:- Met Pro Ser Gly Ala Ser Ser Ser Pro Pro Pr - #o Ala Tyr Thr Ser Ala# 15- Ala Pro Leu Glu Thr Tyr Asn Ser Trp Leu Se - #r Ala Phe Ser Cys Ala# 30- Tyr Pro Gln Cys Thr Ala Gly Arg Gly His Ar - #g Gln Asn Gly Lys Lys# 45- Cys Ile Arg Cys Ile Val Ile Ser Val Cys Se - #r Leu Val Cys Ile Ala# 60- Ala His Leu Ala Val Thr Val Ser Gly Val Al - #a Leu Ile Pro Leu Ile# 80- Asp Gln Asn Arg Ala Tyr Gly Asn Cys Thr Va - #l Cys Val Ile Ala Gly# 95- Phe Ile Ala Thr Phe Ala Ala Arg Leu Thr Il - #e Arg Leu Ser Glu Thr# 110- Leu Met Leu Val Gly Lys Pro Ala Gln Phe Il - #e Phe Ala Ile Ile Ala# 125- Ser Val Ala Glu Thr Leu Ile Asn Asn Glu Al - #a Leu Ala Ile Ser Asn# 140- Thr Thr Tyr Lys Thr Ala Leu Arg Ile Ile Gl - #u Val Thr Ser Leu Ala145 1 - #50 1 - #55 1 -#60- Cys Phe Val Met Leu Gly Ala Ile Ile Thr Se - #r His Asn Tyr Val Cys# 175- Ile Ser Thr Ala Gly Asp Leu Thr Trp Lys Gl - #y Gly Ile Phe His Ala# 190- Tyr His Gly Thr Leu Leu Gly Ile Thr Ile Pr - #o Asn Ile His Pro Ile# 205- Pro Leu Ala Gly Phe Leu Ala Val Tyr Thr Il - #e Leu Ala Ile Asn Ile# 220- Ala Arg Asp Ala Ser Ala Thr Leu Leu Ser Th - #r Cys Tyr Tyr Arg Asn225 2 - #30 2 - #35 2 -#40- Cys Arg Glu Arg Thr Ile Leu Arg Pro Ser Ar - #g Leu Gly His Gly Tyr# 255- Thr Ile Pro Ser Pro Gly Ala Asp Met Leu Ty - #r Glu Glu Asp Val Tyr# 270- Ser Phe Asp Ala Ala Lys Gly His Tyr Ser Se - #r Ile Phe Leu Cys Tyr# 285- Ala Met Gly Leu Thr Thr Pro Leu Ile Ile Al - #a Leu His Lys Tyr Met# 300- Ala Gly Ile Lys Asn Ser Ser Asp Trp Thr Al - #a Thr Leu Gln Gly Met305 3 - #10 3 - #15 3 -#20- Tyr Gly Leu Val Leu Gly Ser Leu Ser Ser Le - #u Cys Ile Pro Ser Ser# 335- Asn Asn Asp Ala Leu Ile Arg Pro Ile Gln Il - #e Leu Ile Leu Ile Ile# 350- Gly Ala Leu Ala Ile Ala Leu Ala Gly Cys Gl - #y Gln Ile Ile Gly Pro# 365- Thr Leu Phe Ala Ala Ser Ser Ala Ala Met Se - #r Cys Phe Thr Cys Ile# 380- Asn Ile Arg Ala Thr Asn Lys Gly Val Asn Ly - #s Leu Ala Ala Ala Ser385 3 - #90 3 - #95 4 -#00- Val Val Lys Ser Val Leu Gly Phe Ile Ile Se - #r Gly Met Leu Thr Cys# 415- Val Leu Leu Pro Leu Ser 420- (2) INFORMATION FOR SEQ ID NO:6:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 489 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:- Met Val Ser Asn Met Arg Val Leu Arg Val Le - #u Arg Leu Thr Gly Trp# 15- Val Gly Ile Phe Leu Val Leu Ser Leu Gln Gl - #n Thr Ser Cys Ala Gly# 30- Leu Pro His Asn Val Asp Thr His His Ile Le - #u Thr Phe Asn Pro Ser# 45- Pro Ile Ser Ala Asp Gly Val Pro Leu Ser Gl - #u Val Pro Asn Ser Pro# 60- Thr Thr Glu Leu Ser Thr Thr Val Ala Thr Ly - #s Thr Ala Val Pro Thr# 80- Thr Glu Ser Thr Ser Ser Ser Glu Ala His Ar - #g Asn Ser Ser His Lys# 95- Ile Pro Asp Ile Ile Cys Asp Arg Glu Glu Va - #l Phe Val Phe Leu Asn# 110- Asn Thr Gly Arg Ile Leu Cys Asp Leu Ile Va - #l Asp Pro Pro Ser Asp# 125- Asp Glu Trp Ser Asn Phe Ala Leu Asp Val Th - #r Phe Asn Pro Ile Glu# 140- Tyr His Ala Asn Glu Lys Asn Val Glu Val Al - #a Arg Val Ala Gly Leu145 1 - #50 1 - #55 1 -#60- Tyr Gly Val Pro Gly Ser Asp Tyr Ala Tyr Pr - #o Arg Lys Ser Glu Leu# 175#Ser Phe Trp Thr Ser Prorg Asp Pro Gln Gly# 190- Thr Pro Arg Gly Asn Lys Tyr Phe Ile Trp Il - #e Asn Lys Thr Met His# 205- Thr Met Gly Val Glu Val Arg Asn Val Asp Ty - #r Lys Asp Asn Gly Tyr# 220- Phe Gln Val Ile Leu Arg Asp Arg Phe Asn Ar - #g Pro Leu Val Glu Lys225 2 - #30 2 - #35 2 -#40- His Ile Tyr Met Arg Val Cys Gln Arg Pro Al - #a Ser Val Asp Val Leu# 255- Ala Pro Pro Val Leu Ser Gly Glu Asn Tyr Ly - #s Ala Ser Cys Ile Val# 270- Arg His Phe Tyr Pro Pro Gly Ser Val Tyr Va - #l Ser Trp Arg Arg Asn# 285- Gly Asn Ile Ala Thr Pro Arg Lys Asp Arg As - #p Gly Ser Phe Trp Trp# 300- Phe Glu Ser Gly Arg Gly Ala Thr Leu Val Se - #r Thr Ile Thr Leu Gly305 3 - #10 3 - #15 3 -#20- Asn Ser Gly Leu Glu Ser Pro Pro Lys Val Se - #r Cys Leu Val Ala Trp# 335- Arg Gln Gly Asp Met Ile Ser Thr Ser Asn Al - #a Thr Ala Val Pro Thr# 350- Val Tyr Tyr His Pro Arg Ile Ser Leu Ala Ph - #e Lys Asp Gly Tyr Ala# 365- Ile Cys Thr Ile Glu Cys Val Pro Ser Gly Il - #e Thr Val Arg Trp Leu# 380- Val His Asp Glu Pro Gln Pro Asn Thr Thr Ty - #r Asp Thr Val Val Thr385 3 - #90 3 - #95 4 -#00- Gly Leu Cys Arg Thr Ile Asp Arg Tyr Arg As - #n Leu Ala Ser Arg Ile# 415- Pro Val Gln Asp Asn Trp Ala Lys Thr Lys Ty - #r Thr Cys Arg Leu Ile# 430- Gly Tyr Pro Phe Asp Val Asp Arg Phe Gln As - #n Ser Glu Tyr Tyr Asp# 445#Ile Val Thr Ile Thr Alarg Gly Met Pro Met# 460- Val Leu Gly Leu Ala Leu Phe Leu Gly Ile Gl - #y Ile Ile Ile Thr Ala465 4 - #70 4 - #75 4 -#80- Leu Cys Phe Tyr Leu Pro Gly Arg Asn 485- (2) INFORMATION FOR SEQ ID NO:7:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 212 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:- Met Met Ser Pro Thr Pro Glu Asp Asp Arg As - #p Leu Val Val Val Arg# 15- Gly Arg Leu Arg Met Met Asp Ser Gly Thr Gl - #u Thr Asp Arg Glu Gln# 30- Arg His Pro Arg Thr Thr Trp Arg Ser Ile Cy - #s Cys Gly Cys Thr Ile# 45- Gly Met Val Phe Thr Ile Phe Val Leu Val Al - #a Ala Val Leu Leu Gly# 60- Ser Leu Phe Thr Val Ser Tyr Met Ala Met Gl - #u Ser Gly Thr Cys Pro# 80- Asp Glu Trp Ile Gly Leu Gly Tyr Ser Cys Me - #t Arg Val Ala Gly Lys# 95- Asn Ala Thr Asp Leu Glu Ala Leu Asp Thr Cy - #s Ala Arg His Asn Ser# 110- Lys Leu Ile Asp Phe Ala Asn Ala Lys Val Le - #u Val Glu Ala Ile Ala# 125- Pro Phe Gly Val Pro Asn Ala Ala Tyr Gly Gl - #u Val Phe Arg Leu Arg# 140- Asp Ser Lys Thr Thr Cys Ile Arg Pro Thr Me - #t Gly Gly Pro Val Ser145 1 - #50 1 - #55 1 -#60- Ala Asp Cys Pro Val Thr Cys Thr Val Ile Cy - #s Gln Arg Pro Arg Pro# 175- Leu Ser Thr Met Ser Ser Ile Ile Arg Asp Al - #a Arg Val Tyr Leu His# 190- Leu Glu Arg Arg Asp Tyr Tyr Glu Val Tyr Al - #a Ser Val Leu Ser Asn# 205- Ala Met Ser Lys 210- (2) INFORMATION FOR SEQ ID NO:8:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1506 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1506- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:- ATG CTC ACG CCG CGT GTG TTA CGA GCT TTG GG - #G TGG ACT GGA CTC TTT 48Met Leu Thr Pro Arg Val Leu Arg Ala Leu Gl - #y Trp Thr Gly Leu Phe# 15- TTT TTG CTT TTA TCT CCG AGC AAC GTC CTA GG - #A GCC AGC CTT AGC CGG 96Phe Leu Leu Leu Ser Pro Ser Asn Val Leu Gl - #y Ala Ser Leu Ser Arg# 30- GAT CTC GAA ACA CCC CCA TTT CTA TCC TTT GA - #T CCA TCC AAC ATT TCA 144Asp Leu Glu Thr Pro Pro Phe Leu Ser Phe As - #p Pro Ser Asn Ile Ser# 45- ATT AAC GGC GCG CCT TTA ACT GAG GTA CCT CA - #T GCA CCT TCC ACA GAA 192Ile Asn Gly Ala Pro Leu Thr Glu Val Pro Hi - #s Ala Pro Ser Thr Glu# 60- AGT GTG TCA ACA AAT TCG GAA AGT ACC AAT GA - #A CAT ACC ATA ACA GAA 240Ser Val Ser Thr Asn Ser Glu Ser Thr Asn Gl - #u His Thr Ile Thr Glu# 80- ACG ACG GGC AAG AAC GCA TAC ATC CAC AAC AA - #T GCG TCT ACG GAC AAG 288Thr Thr Gly Lys Asn Ala Tyr Ile His Asn As - #n Ala Ser Thr Asp Lys# 95- CAA AAT GCG AAC GAC ACT CAT AAA ACG CCC AA - #T ATA CTC TGC GAT ACG 336Gln Asn Ala Asn Asp Thr His Lys Thr Pro As - #n Ile Leu Cys Asp Thr# 110- GAA GAA GTT TTT GTT TTC CTT AAC GAA ACG GG - #A AGA TTT GTT TGT ACT 384Glu Glu Val Phe Val Phe Leu Asn Glu Thr Gl - #y Arg Phe Val Cys Thr# 125- CTC AAA GTC GAC CCC CCC TCG GAT AGT GAA TG - #G TCC AAC TTT GTT CTA 432Leu Lys Val Asp Pro Pro Ser Asp Ser Glu Tr - #p Ser Asn Phe Val Leu# 140- GAT CTG ATC TTT AAC CCA ATT GAA TAC CAC GC - #C AAC GAA AAG AAT GTG 480Asp Leu Ile Phe Asn Pro Ile Glu Tyr His Al - #a Asn Glu Lys Asn Val145 1 - #50 1 - #55 1 -#60- GAA GCG GCG CGT ATC GCT GGT CTC TAT GGA GT - #C CCC GGA TCA GAC TAT 528Glu Ala Ala Arg Ile Ala Gly Leu Tyr Gly Va - #l Pro Gly Ser Asp Tyr# 175- GCA TAC CCA CGT CAA TCT GAA TTA ATT TCT TC - #G ATT CGA CGA GAT CCC 576Ala Tyr Pro Arg Gln Ser Glu Leu Ile Ser Se - #r Ile Arg Arg Asp Pro# 190- CAG GGC ACA TTT TGG ACG AGC CCA TCA CCT CA - #T GGA AAC AAG TAC TTC 624Gln Gly Thr Phe Trp Thr Ser Pro Ser Pro Hi - #s Gly Asn Lys Tyr Phe# 205- ATA TGG ATA AAC AAA ACA ACC AAT ACG ATG GG - #C GTG GAA ATT AGA AAT 672Ile Trp Ile Asn Lys Thr Thr Asn Thr Met Gl - #y Val Glu Ile Arg Asn# 220- GTA GAT TAT GCT GAT AAT GGC TAC ATG CAA GT - #C ATT ATG CGT GAC CAT 720Val Asp Tyr Ala Asp Asn Gly Tyr Met Gln Va - #l Ile Met Arg Asp His225 2 - #30 2 - #35 2 -#40- TTT AAT CGG CCT TTA ATA GAT AAA CAT ATT TA - #C ATA CGT GTG TGT CAA 768Phe Asn Arg Pro Leu Ile Asp Lys His Ile Ty - #r Ile Arg Val Cys Gln# 255- CGA CCT GCA TCA GTG GAT GTA CTG GCC CCT CC - #A GTC CTC AGC GGA GAA 816Arg Pro Ala Ser Val Asp Val Leu Ala Pro Pr - #o Val Leu Ser Gly Glu# 270- AAT TAC AAG GCA TCT TGT ATC GTT AGA CAC TT - #T TAT CCC CCT GGA TCT 864Asn Tyr Lys Ala Ser Cys Ile Val Arg His Ph - #e Tyr Pro Pro Gly Ser# 285- GTC TAT GTA TCT TGG AGA CAG AAT GGA AAC AT - #T GCA ACT CCT CGG AAA 912Val Tyr Val Ser Trp Arg Gln Asn Gly Asn Il - #e Ala Thr Pro Arg Lys# 300- GAT CGC GAT GGA AGT TTT TGG TGG TTC GAA TC - #T GGT AGA GGA GCT ACG 960Asp Arg Asp Gly Ser Phe Trp Trp Phe Glu Se - #r Gly Arg Gly Ala Thr305 3 - #10 3 - #15 3 -#20- TTG GTT TCT ACA ATA ACA TTG GGA AAT TCA GG - #A ATT GAT TTC CCC CCC1008Leu Val Ser Thr Ile Thr Leu Gly Asn Ser Gl - #y Ile Asp Phe Pro Pro# 335- AAA ATA TCT TGT CTG GTT GCC TGG AAG CAG GG - #T GAT ATG ATC AGC ACG1056Lys Ile Ser Cys Leu Val Ala Trp Lys Gln Gl - #y Asp Met Ile Ser Thr# 350- ACG AAT GCC ACA GCT ATC CCG ACG GTA TAT CA - #T CAT CCC CGT TTA TCC1104Thr Asn Ala Thr Ala Ile Pro Thr Val Tyr Hi - #s His Pro Arg Leu Ser# 365- CTG GCT TTT AAA GAT GGG TAT GCA ATA TGT AC - #T ATA GAA TGT GTC CCC1152Leu Ala Phe Lys Asp Gly Tyr Ala Ile Cys Th - #r Ile Glu Cys Val Pro# 380- TCT GAG ATT ACT GTA CGG TGG TTA GTA CAT GA - #T GAA GCG CAG CCT AAC1200#Asp Glu Ala Gln Pro Asn Trp Leu Val His385 3 - #90 3 - #95 4 -#00- ACA ACT TAT AAT ACT GTG GTT ACA GGT CTC TG - #C CGG ACC ATC GAT CGC1248Thr Thr Tyr Asn Thr Val Val Thr Gly Leu Cy - #s Arg Thr Ile Asp Arg# 415- CAT AGA AAT CTC CTC AGC CGC ATT CCA GTA TG - #G GAC AAT TGG ACG AAA1296His Arg Asn Leu Leu Ser Arg Ile Pro Val Tr - #p Asp Asn Trp Thr Lys# 430- ACA AAA TAT ACG TGC AGA CTC ATA GGC TAC CC - #C TTC GAT GAA GAT AAA1344Thr Lys Tyr Thr Cys Arg Leu Ile Gly Tyr Pr - #o Phe Asp Glu Asp Lys# 445- TTT CAA GAT TCG GAA TAT TAC GAT GCA ACT CC - #A TCT GCA AGA GGA ACA1392Phe Gln Asp Ser Glu Tyr Tyr Asp Ala Thr Pr - #o Ser Ala Arg Gly Thr# 460- CCC ATG GTT ATT ACG GTT ACG GCA GTT TTG GG - #A TTG GCT GTA ATT TTA1440Pro Met Val Ile Thr Val Thr Ala Val Leu Gl - #y Leu Ala Val Ile Leu465 4 - #70 4 - #75 4 -#80- GGG ATG GGG ATA ATC ATG ACT GCC CTA TGT TT - #A TAC AAC TCC ACA CGA1488Gly Met Gly Ile Ile Met Thr Ala Leu Cys Le - #u Tyr Asn Ser Thr Arg# 495#1506 TA TAALys Asn Ile Arg Leu 500- (2) INFORMATION FOR SEQ ID NO:9:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 501 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:- Met Leu Thr Pro Arg Val Leu Arg Ala Leu Gl - #y Trp Thr Gly Leu Phe# 15- Phe Leu Leu Leu Ser Pro Ser Asn Val Leu Gl - #y Ala Ser Leu Ser Arg# 30- Asp Leu Glu Thr Pro Pro Phe Leu Ser Phe As - #p Pro Ser Asn Ile Ser# 45- Ile Asn Gly Ala Pro Leu Thr Glu Val Pro Hi - #s Ala Pro Ser Thr Glu# 60- Ser Val Ser Thr Asn Ser Glu Ser Thr Asn Gl - #u His Thr Ile Thr Glu# 80- Thr Thr Gly Lys Asn Ala Tyr Ile His Asn As - #n Ala Ser Thr Asp Lys# 95- Gln Asn Ala Asn Asp Thr His Lys Thr Pro As - #n Ile Leu Cys Asp Thr# 110- Glu Glu Val Phe Val Phe Leu Asn Glu Thr Gl - #y Arg Phe Val Cys Thr# 125- Leu Lys Val Asp Pro Pro Ser Asp Ser Glu Tr - #p Ser Asn Phe Val Leu# 140- Asp Leu Ile Phe Asn Pro Ile Glu Tyr His Al - #a Asn Glu Lys Asn Val145 1 - #50 1 - #55 1 -#60- Glu Ala Ala Arg Ile Ala Gly Leu Tyr Gly Va - #l Pro Gly Ser Asp Tyr# 175- Ala Tyr Pro Arg Gln Ser Glu Leu Ile Ser Se - #r Ile Arg Arg Asp Pro# 190- Gln Gly Thr Phe Trp Thr Ser Pro Ser Pro Hi - #s Gly Asn Lys Tyr Phe# 205#Gly Val Glu Ile Arg Asnhr Thr Asn Thr Met# 220- Val Asp Tyr Ala Asp Asn Gly Tyr Met Gln Va - #l Ile Met Arg Asp His225 2 - #30 2 - #35 2 -#40- Phe Asn Arg Pro Leu Ile Asp Lys His Ile Ty - #r Ile Arg Val Cys Gln# 255- Arg Pro Ala Ser Val Asp Val Leu Ala Pro Pr - #o Val Leu Ser Gly Glu# 270- Asn Tyr Lys Ala Ser Cys Ile Val Arg His Ph - #e Tyr Pro Pro Gly Ser# 285- Val Tyr Val Ser Trp Arg Gln Asn Gly Asn Il - #e Ala Thr Pro Arg Lys# 300- Asp Arg Asp Gly Ser Phe Trp Trp Phe Glu Se - #r Gly Arg Gly Ala Thr305 3 - #10 3 - #15 3 -#20- Leu Val Ser Thr Ile Thr Leu Gly Asn Ser Gl - #y Ile Asp Phe Pro Pro# 335- Lys Ile Ser Cys Leu Val Ala Trp Lys Gln Gl - #y Asp Met Ile Ser Thr# 350- Thr Asn Ala Thr Ala Ile Pro Thr Val Tyr Hi - #s His Pro Arg Leu Ser# 365- Leu Ala Phe Lys Asp Gly Tyr Ala Ile Cys Th - #r Ile Glu Cys Val Pro# 380- Ser Glu Ile Thr Val Arg Trp Leu Val His As - #p Glu Ala Gln Pro Asn385 3 - #90 3 - #95 4 -#00- Thr Thr Tyr Asn Thr Val Val Thr Gly Leu Cy - #s Arg Thr Ile Asp Arg# 415- His Arg Asn Leu Leu Ser Arg Ile Pro Val Tr - #p Asp Asn Trp Thr Lys# 430- Thr Lys Tyr Thr Cys Arg Leu Ile Gly Tyr Pr - #o Phe Asp Glu Asp Lys# 445- Phe Gln Asp Ser Glu Tyr Tyr Asp Ala Thr Pr - #o Ser Ala Arg Gly Thr# 460- Pro Met Val Ile Thr Val Thr Ala Val Leu Gl - #y Leu Ala Val Ile Leu465 4 - #70 4 - #75 4 -#80- Gly Met Gly Ile Ile Met Thr Ala Leu Cys Le - #u Tyr Asn Ser Thr Arg# 495- Lys Asn Ile Arg Leu 500- (2) INFORMATION FOR SEQ ID NO:10:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1734 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1734- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:- ATG GAC CGC GCC GTT AGC CAA GTT GCG TTA GA - #G AAT GAT GAA AGA GAG 48Met Asp Arg Ala Val Ser Gln Val Ala Leu Gl - #u Asn Asp Glu Arg Glu# 15- GCA AAA AAT ACA TGG CGC TTG ATA TTC CGG AT - #T GCA ATC TTA TTC TTA 96Ala Lys Asn Thr Trp Arg Leu Ile Phe Arg Il - #e Ala Ile Leu Phe Leu# 30- ACA GTA GTG ACC TTG GCT ATA TCT GTA GCC TC - #C CTT TTA TAT AGC ATG 144Thr Val Val Thr Leu Ala Ile Ser Val Ala Se - #r Leu Leu Tyr Ser Met# 45- GGG GCT AGC ACA CCT AGC GAT CTT GTA GGC AT - #A CCG ACT AGG ATT TCC 192#Ile Pro Thr Arg Ile Ser Asp Leu Val Gly# 60- AGG GCA GAA GAA AAG ATT ACA TCT ACA CTT GG - #T TCC AAT CAA GAT GTA 240Arg Ala Glu Glu Lys Ile Thr Ser Thr Leu Gl - #y Ser Asn Gln Asp Val# 80- GTA GAT AGG ATA TAT AAG CAA GTG GCC CTT GA - #G TCT CCA TTG GCA TTG 288Val Asp Arg Ile Tyr Lys Gln Val Ala Leu Gl - #u Ser Pro Leu Ala Leu# 95- TTA AAT ACT GAG ACC ACA ATT ATG AAC GCA AT - #A ACA TCT CTC TCT TAT 336Leu Asn Thr Glu Thr Thr Ile Met Asn Ala Il - #e Thr Ser Leu Ser Tyr# 110- CAG ATT AAT GGA GCT GCA AAC AAC AGC GGG TG - #G GGG GCA CCT ATT CAT 384Gln Ile Asn Gly Ala Ala Asn Asn Ser Gly Tr - #p Gly Ala Pro Ile His# 125- GAC CCA GAT TAT ATA GGG GGG ATA GGC AAA GA - #A CTC ATT GTA GAT GAT 432Asp Pro Asp Tyr Ile Gly Gly Ile Gly Lys Gl - #u Leu Ile Val Asp Asp# 140- GCT AGT GAT GTC ACA TCA TTC TAT CCC TCT GC - #A TTT CAA GAA CAT CTG 480Ala Ser Asp Val Thr Ser Phe Tyr Pro Ser Al - #a Phe Gln Glu His Leu145 1 - #50 1 - #55 1 -#60- AAT TTT ATC CCG GCG CCT ACT ACA GGA TCA GG - #T TGC ACT CGA ATA CCC 528Asn Phe Ile Pro Ala Pro Thr Thr Gly Ser Gl - #y Cys Thr Arg Ile Pro# 175- TCA TTT GAC ATG AGT GCT ACC CAT TAC TGC TA - #C ACC CAT AAT GTA ATA 576Ser Phe Asp Met Ser Ala Thr His Tyr Cys Ty - #r Thr His Asn Val Ile# 190- TTG TCT GGA TGC AGA GAT CAC TCA CAC TCA CA - #T CAG TAT TTA GCA CTT 624Leu Ser Gly Cys Arg Asp His Ser His Ser Hi - #s Gln Tyr Leu Ala Leu# 205- GGT GTG CTC CGG ACA TCT GCA ACA GGG AGG GT - #A TTC TTT TCT ACT CTG 672Gly Val Leu Arg Thr Ser Ala Thr Gly Arg Va - #l Phe Phe Ser Thr Leu# 220- CGT TCC ATC AAC CTG GAC GAC ACC CAA AAT CG - #G AAG TCT TGC AGT GTG 720Arg Ser Ile Asn Leu Asp Asp Thr Gln Asn Ar - #g Lys Ser Cys Ser Val225 2 - #30 2 - #35 2 -#40- AGT GCA ACT CCC CTG GGT TGT GAT ATG CTG TG - #C TCG AAA GCC ACG GAG 768Ser Ala Thr Pro Leu Gly Cys Asp Met Leu Cy - #s Ser Lys Ala Thr Glu# 255- ACA GAG GAA GAA GAT TAT AAC TCA GCT GTC CC - #T ACG CGG ATG GTA CAT 816Thr Glu Glu Glu Asp Tyr Asn Ser Ala Val Pr - #o Thr Arg Met Val His# 270- GGG AGG TTA GGG TTC GAC GGC CAA TAT CAC GA - #A AAG GAC CTA GAT GTC 864Gly Arg Leu Gly Phe Asp Gly Gln Tyr His Gl - #u Lys Asp Leu Asp Val# 285#TAC CCA GGA GTA GGG GGT 912TG GCC AACThr Thr Leu Phe Gly Asp Trp Val Ala Asn Ty - #r Pro Gly Val Gly Gly# 300- GGA TCT TTT ATT GAC AGC CGC GTG TGG TTC TC - #A GTC TAC GGA GGG TTA 960Gly Ser Phe Ile Asp Ser Arg Val Trp Phe Se - #r Val Tyr Gly Gly Leu305 3 - #10 3 - #15 3 -#20- AAA CCC AAT ACA CCC AGT GAC ACT GTA CAG GA - #A GGG AAA TAT GTG ATA1008Lys Pro Asn Thr Pro Ser Asp Thr Val Gln Gl - #u Gly Lys Tyr Val Ile# 335- TAC AAG CGA TAC AAT GAC ACA TGC CCA GAT GA - #G CAA GAC TAC CAG ATT1056Tyr Lys Arg Tyr Asn Asp Thr Cys Pro Asp Gl - #u Gln Asp Tyr Gln Ile# 350- CGA ATG GCC AAG TCT TCG TAT AAG CCT GGA CG - #G TTT GGT GGG AAA CGC1104Arg Met Ala Lys Ser Ser Tyr Lys Pro Gly Ar - #g Phe Gly Gly Lys Arg# 365- ATA CAG CAG GCT ATC TTA TCT ATC AAA GTG TC - #A ACA TCC TTA GGC GAA1152Ile Gln Gln Ala Ile Leu Ser Ile Lys Val Se - #r Thr Ser Leu Gly Glu# 380- GAC CCG GTA CTG ACT GTA CCG CCC AAC ACA GT - #C ACA CTC ATG GGG GCC1200Asp Pro Val Leu Thr Val Pro Pro Asn Thr Va - #l Thr Leu Met Gly Ala385 3 - #90 3 - #95 4 -#00- GAA GGC AGA ATT CTC ACA GTA GGG ACA TCC CA - #T TTC TTG TAT CAG CGA1248Glu Gly Arg Ile Leu Thr Val Gly Thr Ser Hi - #s Phe Leu Tyr Gln Arg# 415- GGG TCA TCA TAC TTC TCT CCC GCG TTA TTA TA - #T CCT ATG ACA GTC AGC1296Gly Ser Ser Tyr Phe Ser Pro Ala Leu Leu Ty - #r Pro Met Thr Val Ser# 430- AAC AAA ACA GCC ACT CTT CAT AGT CCT TAT AC - #A TTC AAT GCC TTC ACT1344Asn Lys Thr Ala Thr Leu His Ser Pro Tyr Th - #r Phe Asn Ala Phe Thr# 445- CGG CCA GGT AGT ATC CCT TGC CAG GCT TCA GC - #A AGA TGC CCC AAC TCA1392Arg Pro Gly Ser Ile Pro Cys Gln Ala Ser Al - #a Arg Cys Pro Asn Ser# 460- TGT GTT ACT GGA GTC TAT ACA GAT CCA TAT CC - #C CTA ATC TTC TAT AGA1440Cys Val Thr Gly Val Tyr Thr Asp Pro Tyr Pr - #o Leu Ile Phe Tyr Arg465 4 - #70 4 - #75 4 -#80- AAC CAC ACC TTG CGA GGG GTA TTC GGG ACA AT - #G CTT GAT GGT GAA CAA1488Asn His Thr Leu Arg Gly Val Phe Gly Thr Me - #t Leu Asp Gly Glu Gln# 495- GCA AGA CTT AAC CCT GCG TCT GCA GTA TTC GA - #T AGC ACA TCC CGC AGT1536Ala Arg Leu Asn Pro Ala Ser Ala Val Phe As - #p Ser Thr Ser Arg Ser# 510- CGC ATA ACT CGA GTG AGT TCA AGC AGC ATC AA - #A GCA GCA TAC ACA ACA1584Arg Ile Thr Arg Val Ser Ser Ser Ser Ile Ly - #s Ala Ala Tyr Thr Thr# 525- TCA ACT TGT TTT AAA GTG GTC AAG ACC AAT AA - #G ACC TAT TGT CTC AGC1632Ser Thr Cys Phe Lys Val Val Lys Thr Asn Ly - #s Thr Tyr Cys Leu Ser# 540- ATT GCT GAA ATA TCT AAT ACT CTC TTC GGA GA - #A TTC AGA ATC GTC CCG1680Ile Ala Glu Ile Ser Asn Thr Leu Phe Gly Gl - #u Phe Arg Ile Val Pro545 5 - #50 5 - #55 5 -#60- TTA CTA GTT GAG ATC CTC AAA GAT GAC GGG GT - #T AGA GAA GCC AGG TCT1728Leu Leu Val Glu Ile Leu Lys Asp Asp Gly Va - #l Arg Glu Ala Arg Ser# 575# 1734Gly- (2) INFORMATION FOR SEQ ID NO:11:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 577 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:- Met Asp Arg Ala Val Ser Gln Val Ala Leu Gl - #u Asn Asp Glu Arg Glu# 15- Ala Lys Asn Thr Trp Arg Leu Ile Phe Arg Il - #e Ala Ile Leu Phe Leu# 30- Thr Val Val Thr Leu Ala Ile Ser Val Ala Se - #r Leu Leu Tyr Ser Met# 45- Gly Ala Ser Thr Pro Ser Asp Leu Val Gly Il - #e Pro Thr Arg Ile Ser# 60- Arg Ala Glu Glu Lys Ile Thr Ser Thr Leu Gl - #y Ser Asn Gln Asp Val# 80- Val Asp Arg Ile Tyr Lys Gln Val Ala Leu Gl - #u Ser Pro Leu Ala Leu# 95- Leu Asn Thr Glu Thr Thr Ile Met Asn Ala Il - #e Thr Ser Leu Ser Tyr# 110- Gln Ile Asn Gly Ala Ala Asn Asn Ser Gly Tr - #p Gly Ala Pro Ile His# 125- Asp Pro Asp Tyr Ile Gly Gly Ile Gly Lys Gl - #u Leu Ile Val Asp Asp# 140- Ala Ser Asp Val Thr Ser Phe Tyr Pro Ser Al - #a Phe Gln Glu His Leu145 1 - #50 1 - #55 1 -#60- Asn Phe Ile Pro Ala Pro Thr Thr Gly Ser Gl - #y Cys Thr Arg Ile Pro# 175- Ser Phe Asp Met Ser Ala Thr His Tyr Cys Ty - #r Thr His Asn Val Ile# 190- Leu Ser Gly Cys Arg Asp His Ser His Ser Hi - #s Gln Tyr Leu Ala Leu# 205- Gly Val Leu Arg Thr Ser Ala Thr Gly Arg Va - #l Phe Phe Ser Thr Leu# 220- Arg Ser Ile Asn Leu Asp Asp Thr Gln Asn Ar - #g Lys Ser Cys Ser Val225 2 - #30 2 - #35 2 -#40- Ser Ala Thr Pro Leu Gly Cys Asp Met Leu Cy - #s Ser Lys Ala Thr Glu# 255- Thr Glu Glu Glu Asp Tyr Asn Ser Ala Val Pr - #o Thr Arg Met Val His# 270- Gly Arg Leu Gly Phe Asp Gly Gln Tyr His Gl - #u Lys Asp Leu Asp Val# 285- Thr Thr Leu Phe Gly Asp Trp Val Ala Asn Ty - #r Pro Gly Val Gly Gly# 300- Gly Ser Phe Ile Asp Ser Arg Val Trp Phe Se - #r Val Tyr Gly Gly Leu305 3 - #10 3 - #15 3 -#20- Lys Pro Asn Thr Pro Ser Asp Thr Val Gln Gl - #u Gly Lys Tyr Val Ile# 335- Tyr Lys Arg Tyr Asn Asp Thr Cys Pro Asp Gl - #u Gln Asp Tyr Gln Ile# 350- Arg Met Ala Lys Ser Ser Tyr Lys Pro Gly Ar - #g Phe Gly Gly Lys Arg# 365- Ile Gln Gln Ala Ile Leu Ser Ile Lys Val Se - #r Thr Ser Leu Gly Glu# 380- Asp Pro Val Leu Thr Val Pro Pro Asn Thr Va - #l Thr Leu Met Gly Ala385 3 - #90 3 - #95 4 -#00- Glu Gly Arg Ile Leu Thr Val Gly Thr Ser Hi - #s Phe Leu Tyr Gln Arg# 415- Gly Ser Ser Tyr Phe Ser Pro Ala Leu Leu Ty - #r Pro Met Thr Val Ser# 430- Asn Lys Thr Ala Thr Leu His Ser Pro Tyr Th - #r Phe Asn Ala Phe Thr# 445- Arg Pro Gly Ser Ile Pro Cys Gln Ala Ser Al - #a Arg Cys Pro Asn Ser# 460- Cys Val Thr Gly Val Tyr Thr Asp Pro Tyr Pr - #o Leu Ile Phe Tyr Arg465 4 - #70 4 - #75 4 -#80- Asn His Thr Leu Arg Gly Val Phe Gly Thr Me - #t Leu Asp Gly Glu Gln# 495- Ala Arg Leu Asn Pro Ala Ser Ala Val Phe As - #p Ser Thr Ser Arg Ser# 510- Arg Ile Thr Arg Val Ser Ser Ser Ser Ile Ly - #s Ala Ala Tyr Thr Thr# 525- Ser Thr Cys Phe Lys Val Val Lys Thr Asn Ly - #s Thr Tyr Cys Leu Ser# 540- Ile Ala Glu Ile Ser Asn Thr Leu Phe Gly Gl - #u Phe Arg Ile Val Pro545 5 - #50 5 - #55 5 -#60- Leu Leu Val Glu Ile Leu Lys Asp Asp Gly Va - #l Arg Glu Ala Arg Ser# 575- Gly- (2) INFORMATION FOR SEQ ID NO:12:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1662 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1662- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:- ATG GGC TCC AGA CCT TCT ACC AAG AAC CCA GC - #A CCT ATG ATG CTG ACT 48Met Gly Ser Arg Pro Ser Thr Lys Asn Pro Al - #a Pro Met Met Leu Thr# 15- ATC CGG GTC GCG CTG GTA CTG AGT TGC ATC TG - #T CCG GCA AAC TCC ATT 96Ile Arg Val Ala Leu Val Leu Ser Cys Ile Cy - #s Pro Ala Asn Ser Ile# 30- GAT GGC AGG CCT CTT GCA GCT GCA GGA ATT GT - #G GTT ACA GGA GAC AAA 144Asp Gly Arg Pro Leu Ala Ala Ala Gly Ile Va - #l Val Thr Gly Asp Lys# 45- GCA GTC AAC ATA TAC ACC TCA TCC CAG ACA GG - #A TCA ATC ATA GTT AAG 192Ala Val Asn Ile Tyr Thr Ser Ser Gln Thr Gl - #y Ser Ile Ile Val Lys# 60- CTC CTC CCG AAT CTG CCA AAG GAT AAG GAG GC - #A TGT GCG AAA GCC CCC 240Leu Leu Pro Asn Leu Pro Lys Asp Lys Glu Al - #a Cys Ala Lys Ala Pro# 80- TTG GAT GCA TAC AAC AGG ACA TTG ACC ACT TT - #G CTC ACC CCC CTT GGT 288Leu Asp Ala Tyr Asn Arg Thr Leu Thr Thr Le - #u Leu Thr Pro Leu Gly# 95- GAC TCT ATC CGT AGG ATA CAA GAG TCT GTG AC - #T ACA TCT GGA GGG GGG 336Asp Ser Ile Arg Arg Ile Gln Glu Ser Val Th - #r Thr Ser Gly Gly Gly# 110- AGA CAG GGG CGC CTT ATA GGC GCC ATT ATT GG - #C GGT GTG GCT CTT GGG 384Arg Gln Gly Arg Leu Ile Gly Ala Ile Ile Gl - #y Gly Val Ala Leu Gly# 125- GTT GCA ACT GCC GCA CAA ATA ACA GCG GCC GC - #A GCT CTG ATA CAA GCC 432Val Ala Thr Ala Ala Gln Ile Thr Ala Ala Al - #a Ala Leu Ile Gln Ala# 140- AAA CAA AAT GCT GCC AAC ATC CTC CGA CTT AA - #A GAG AGC ATT GCC GCA 480Lys Gln Asn Ala Ala Asn Ile Leu Arg Leu Ly - #s Glu Ser Ile Ala Ala145 1 - #50 1 - #55 1 -#60- ACC AAT GAG GCT GTG CAT GAG GTC ACT GAC GG - #A TTA TCG CAA CTA GCA 528Thr Asn Glu Ala Val His Glu Val Thr Asp Gl - #y Leu Ser Gln Leu Ala# 175- GTG GCA GTT GGG AAG ATG CAG CAG TTC GTT AA - #T GAC CAA TTT AAT AAA 576Val Ala Val Gly Lys Met Gln Gln Phe Val As - #n Asp Gln Phe Asn Lys# 190- ACA GCT CAG GAA TTA GAC TGC ATC AAA ATT GC - #A CAG CAA GTT GGT GTA 624Thr Ala Gln Glu Leu Asp Cys Ile Lys Ile Al - #a Gln Gln Val Gly Val# 205- GAG CTC AAC CTG TAC CTA ACC GAA TCG ACT AC - #A GTA TTC GGA CCA CAA 672Glu Leu Asn Leu Tyr Leu Thr Glu Ser Thr Th - #r Val Phe Gly Pro Gln# 220- ATC ACT TCA CCT GCC TTA AAC AAG CTG ACT AT - #T CAG GCA CTT TAC AAT 720Ile Thr Ser Pro Ala Leu Asn Lys Leu Thr Il - #e Gln Ala Leu Tyr Asn225 2 - #30 2 - #35 2 -#40- CTA GCT GGT GGG AAT ATG GAT TAC TTA TTG AC - #T AAG TTA GGT ATA GGG 768Leu Ala Gly Gly Asn Met Asp Tyr Leu Leu Th - #r Lys Leu Gly Ile Gly# 255- AAC AAT CAA CTC AGC TCA TTA ATC GGT AGC GG - #C TTA ATC ACC GGT AAC 816Asn Asn Gln Leu Ser Ser Leu Ile Gly Ser Gl - #y Leu Ile Thr Gly Asn# 270- CCT ATT CTA TAC GAC TCA CAG ACT CAA CTC TT - #G GGT ATA CAG GTA ACT 864Pro Ile Leu Tyr Asp Ser Gln Thr Gln Leu Le - #u Gly Ile Gln Val Thr# 285- CTA CCT TCA GTC GGG AAC CTA AAT AAT ATG CG - #T GCC ACC TAC TTG GAA 912Leu Pro Ser Val Gly Asn Leu Asn Asn Met Ar - #g Ala Thr Tyr Leu Glu# 300- ACC TTA TCC GTA AGC ACA ACC AGG GGA TTT GC - #C TCG GCA CTT GTC CCA 960Thr Leu Ser Val Ser Thr Thr Arg Gly Phe Al - #a Ser Ala Leu Val Pro305 3 - #10 3 - #15 3 -#20- AAA GTG GTG ACA CGG GTC GGT TCT GTG ATA GA - #A GAA CTT GAC ACC TCA1008Lys Val Val Thr Arg Val Gly Ser Val Ile Gl - #u Glu Leu Asp Thr Ser# 335- TAC TGT ATA GAA ACT GAC TTA GAT TTA TAT TG - #T ACA AGA ATA GTA ACG1056Tyr Cys Ile Glu Thr Asp Leu Asp Leu Tyr Cy - #s Thr Arg Ile Val Thr# 350- TTC CCT ATG TCC CCT GGT ATT TAC TCC TGC TT - #G AGC GGC AAT ACA TCG1104Phe Pro Met Ser Pro Gly Ile Tyr Ser Cys Le - #u Ser Gly Asn Thr Ser# 365- GCC TGT ATG TAC TCA AAG ACC GAA GGC GCA CT - #T ACT ACA CCA TAT ATG1152Ala Cys Met Tyr Ser Lys Thr Glu Gly Ala Le - #u Thr Thr Pro Tyr Met# 380- ACT ATC AAA GGC TCA GTC ATC GCT AAC TGC AA - #G ATG ACA ACA TGT AGA1200Thr Ile Lys Gly Ser Val Ile Ala Asn Cys Ly - #s Met Thr Thr Cys Arg385 3 - #90 3 - #95 4 -#00- TGT GTA AAC CCC CCG GGT ATC ATA TCG CAA AA - #C TAT GGA GAA GCC GTG1248Cys Val Asn Pro Pro Gly Ile Ile Ser Gln As - #n Tyr Gly Glu Ala Val# 415- TCT CTA ATA GAT AAA CAA TCA TGC AAT GTT TT - #A TCC TTA GGC GGG ATA1296Ser Leu Ile Asp Lys Gln Ser Cys Asn Val Le - #u Ser Leu Gly Gly Ile# 430- ACT TTA AGG CTC AGT GGG GAA TTC GAT GTA AC - #T TAT CAG AAG AAT ATC1344Thr Leu Arg Leu Ser Gly Glu Phe Asp Val Th - #r Tyr Gln Lys Asn Ile# 445- TCA ATA CAA GAT TCT CAA GTA ATA ATA ACA GG - #C AAT CTT GAT ATC TCA1392Ser Ile Gln Asp Ser Gln Val Ile Ile Thr Gl - #y Asn Leu Asp Ile Ser# 460- ACT GAG CTT GGG AAT GTC AAC AAC TCG ATC AG - #T AAT GCC TTG AAT AAG1440Thr Glu Leu Gly Asn Val Asn Asn Ser Ile Se - #r Asn Ala Leu Asn Lys465 4 - #70 4 - #75 4 -#80- TTA GAG GAA AGC AAC AGA AAA CTA GAC AAA GT - #C AAT GTC AAA CTG ACC1488Leu Glu Glu Ser Asn Arg Lys Leu Asp Lys Va - #l Asn Val Lys Leu Thr# 495- AGC ACA TCT GCT CTC ATT ACC TAT ATC GTT TT - #G ACT ATC ATA TCT CTT1536Ser Thr Ser Ala Leu Ile Thr Tyr Ile Val Le - #u Thr Ile Ile Ser Leu# 510- GTT TTT GGT ATA CTT AGC CTG ATT CTA GCA TG - #C TAC CTA ATG TAC AAG1584Val Phe Gly Ile Leu Ser Leu Ile Leu Ala Cy - #s Tyr Leu Met Tyr Lys# 525- CAA AAG GCG CAA CAA AAG ACC TTA TTA TGG CT - #T GGG AAT AAT ACC CTA1632Gln Lys Ala Gln Gln Lys Thr Leu Leu Trp Le - #u Gly Asn Asn Thr Leu# 540# 1662 GCC ACT ACA AAA ATG TGAAsp Gln Met Arg Ala Thr Thr Lys Met545 5 - #50- (2) INFORMATION FOR SEQ ID NO:13:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 553 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:- Met Gly Ser Arg Pro Ser Thr Lys Asn Pro Al - #a Pro Met Met Leu Thr# 15- Ile Arg Val Ala Leu Val Leu Ser Cys Ile Cy - #s Pro Ala Asn Ser Ile# 30- Asp Gly Arg Pro Leu Ala Ala Ala Gly Ile Va - #l Val Thr Gly Asp Lys# 45- Ala Val Asn Ile Tyr Thr Ser Ser Gln Thr Gl - #y Ser Ile Ile Val Lys# 60- Leu Leu Pro Asn Leu Pro Lys Asp Lys Glu Al - #a Cys Ala Lys Ala Pro# 80- Leu Asp Ala Tyr Asn Arg Thr Leu Thr Thr Le - #u Leu Thr Pro Leu Gly# 95- Asp Ser Ile Arg Arg Ile Gln Glu Ser Val Th - #r Thr Ser Gly Gly Gly# 110- Arg Gln Gly Arg Leu Ile Gly Ala Ile Ile Gl - #y Gly Val Ala Leu Gly# 125- Val Ala Thr Ala Ala Gln Ile Thr Ala Ala Al - #a Ala Leu Ile Gln Ala# 140- Lys Gln Asn Ala Ala Asn Ile Leu Arg Leu Ly - #s Glu Ser Ile Ala Ala145 1 - #50 1 - #55 1 -#60- Thr Asn Glu Ala Val His Glu Val Thr Asp Gl - #y Leu Ser Gln Leu Ala# 175- Val Ala Val Gly Lys Met Gln Gln Phe Val As - #n Asp Gln Phe Asn Lys# 190- Thr Ala Gln Glu Leu Asp Cys Ile Lys Ile Al - #a Gln Gln Val Gly Val# 205- Glu Leu Asn Leu Tyr Leu Thr Glu Ser Thr Th - #r Val Phe Gly Pro Gln# 220- Ile Thr Ser Pro Ala Leu Asn Lys Leu Thr Il - #e Gln Ala Leu Tyr Asn225 2 - #30 2 - #35 2 -#40- Leu Ala Gly Gly Asn Met Asp Tyr Leu Leu Th - #r Lys Leu Gly Ile Gly# 255- Asn Asn Gln Leu Ser Ser Leu Ile Gly Ser Gl - #y Leu Ile Thr Gly Asn# 270- Pro Ile Leu Tyr Asp Ser Gln Thr Gln Leu Le - #u Gly Ile Gln Val Thr# 285- Leu Pro Ser Val Gly Asn Leu Asn Asn Met Ar - #g Ala Thr Tyr Leu Glu# 300- Thr Leu Ser Val Ser Thr Thr Arg Gly Phe Al - #a Ser Ala Leu Val Pro305 3 - #10 3 - #15 3 -#20- Lys Val Val Thr Arg Val Gly Ser Val Ile Gl - #u Glu Leu Asp Thr Ser# 335- Tyr Cys Ile Glu Thr Asp Leu Asp Leu Tyr Cy - #s Thr Arg Ile Val Thr# 350- Phe Pro Met Ser Pro Gly Ile Tyr Ser Cys Le - #u Ser Gly Asn Thr Ser# 365- Ala Cys Met Tyr Ser Lys Thr Glu Gly Ala Le - #u Thr Thr Pro Tyr Met# 380- Thr Ile Lys Gly Ser Val Ile Ala Asn Cys Ly - #s Met Thr Thr Cys Arg385 3 - #90 3 - #95 4 -#00- Cys Val Asn Pro Pro Gly Ile Ile Ser Gln As - #n Tyr Gly Glu Ala Val# 415- Ser Leu Ile Asp Lys Gln Ser Cys Asn Val Le - #u Ser Leu Gly Gly Ile# 430- Thr Leu Arg Leu Ser Gly Glu Phe Asp Val Th - #r Tyr Gln Lys Asn Ile# 445- Ser Ile Gln Asp Ser Gln Val Ile Ile Thr Gl - #y Asn Leu Asp Ile Ser# 460- Thr Glu Leu Gly Asn Val Asn Asn Ser Ile Se - #r Asn Ala Leu Asn Lys465 4 - #70 4 - #75 4 -#80- Leu Glu Glu Ser Asn Arg Lys Leu Asp Lys Va - #l Asn Val Lys Leu Thr# 495- Ser Thr Ser Ala Leu Ile Thr Tyr Ile Val Le - #u Thr Ile Ile Ser Leu# 510- Val Phe Gly Ile Leu Ser Leu Ile Leu Ala Cy - #s Tyr Leu Met Tyr Lys# 525- Gln Lys Ala Gln Gln Lys Thr Leu Leu Trp Le - #u Gly Asn Asn Thr Leu# 540- Asp Gln Met Arg Ala Thr Thr Lys Met545 5 - #50- (2) INFORMATION FOR SEQ ID NO:14:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 3489 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..3489- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:- ATG TTG GTA ACA CCT CTT TTA CTA GTG ACT CT - #T TTG TGT GTA CTA TGT 48Met Leu Val Thr Pro Leu Leu Leu Val Thr Le - #u Leu Cys Val Leu Cys# 15- AGT GCT GCT TTG TAT GAC AGT AGT TCT TAC GT - #T TAC TAC TAC CAA AGT 96Ser Ala Ala Leu Tyr Asp Ser Ser Ser Tyr Va - #l Tyr Tyr Tyr Gln Ser# 30- GCC TTT AGA CCA CCT AAT GGT TGG CAT TTA CA - #C GGG GGT GCT TAT GCG 144Ala Phe Arg Pro Pro Asn Gly Trp His Leu Hi - #s Gly Gly Ala Tyr Ala# 45- GTA GTT AAT ATT TCT AGC GAA TCT AAT AAT GC - #A GGC TCT TCA CCT GGG 192Val Val Asn Ile Ser Ser Glu Ser Asn Asn Al - #a Gly Ser Ser Pro Gly# 60- TGT ATT GTT GGT ACT ATT CAT GGT GGT CGT GT - #T GTT AAT GCT TCT TCT 240Cys Ile Val Gly Thr Ile His Gly Gly Arg Va - #l Val Asn Ala Ser Ser# 80- ATA GCT ATG ACG GCA CCG TCA TCA GGT ATG GC - #T TGG TCT AGC AGT CAG 288Ile Ala Met Thr Ala Pro Ser Ser Gly Met Al - #a Trp Ser Ser Ser Gln# 95- TTT TGT ACT GCA CAC TGT AAC TTT TCA GAT AC - #T ACA GTG TTT GTT ACA 336Phe Cys Thr Ala His Cys Asn Phe Ser Asp Th - #r Thr Val Phe Val Thr# 110- CAT TGT TAT AAA TAT GAT GGG TGT CCT ATA AC - #T GGC ATG CTT CAA AAG 384His Cys Tyr Lys Tyr Asp Gly Cys Pro Ile Th - #r Gly Met Leu Gln Lys# 125- AAT TTT TTA CGT GTT TCT GCT ATG AAA AAT GG - #C CAG CTT TTC TAT AAT 432Asn Phe Leu Arg Val Ser Ala Met Lys Asn Gl - #y Gln Leu Phe Tyr Asn# 140- TTA ACA GTT AGT GTA GCT AAG TAC CCT ACT TT - #T AAA TCA TTT CAG TGT 480Leu Thr Val Ser Val Ala Lys Tyr Pro Thr Ph - #e Lys Ser Phe Gln Cys145 1 - #50 1 - #55 1 -#60- GTT AAT AAT TTA ACA TCC GTA TAT TTA AAT GG - #T GAT CTT GTT TAC ACC 528Val Asn Asn Leu Thr Ser Val Tyr Leu Asn Gl - #y Asp Leu Val Tyr Thr# 175- TCT AAT GAG ACC ACA GAT GTT ACA TCT GCA GG - #T GTT TAT TTT AAA GCT 576Ser Asn Glu Thr Thr Asp Val Thr Ser Ala Gl - #y Val Tyr Phe Lys Ala# 190- GGT GGA CCT ATA ACT TAT AAA GTT ATG AGA AA - #A GTT AAA GCC CTG GCT 624Gly Gly Pro Ile Thr Tyr Lys Val Met Arg Ly - #s Val Lys Ala Leu Ala# 205- TAT TTT GTT AAT GGT ACT GCA CAA GAT GTT AT - #T TTG TGT GAT GGA TCA 672Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Il - #e Leu Cys Asp Gly Ser# 220- CCT AGA GGC TTG TTA GCA TGC CAG TAT AAT AC - #T GGC AAT TTT TCA GAT 720Pro Arg Gly Leu Leu Ala Cys Gln Tyr Asn Th - #r Gly Asn Phe Ser Asp225 2 - #30 2 - #35 2 -#40- GGC TTT TAT CCT TTT ATT AAT AGT AGT TTA GT - #T AAG CAG AAG TTT ATT 768Gly Phe Tyr Pro Phe Ile Asn Ser Ser Leu Va - #l Lys Gln Lys Phe Ile# 255#TTT ACG TTA CAC AAT TTC 816AT ACT ACTVal Tyr Arg Glu Asn Ser Val Asn Thr Thr Ph - #e Thr Leu His Asn Phe# 270- ACT TTT CAT AAT GAG ACT GGC GCC AAC CCT AA - #T CCT AGT GGT GTT CAG 864Thr Phe His Asn Glu Thr Gly Ala Asn Pro As - #n Pro Ser Gly Val Gln# 285- AAT ATT CTA ACT TAC CAA ACA CAA ACA GCT CA - #G AGT GGT TAT TAT AAT 912Asn Ile Leu Thr Tyr Gln Thr Gln Thr Ala Gl - #n Ser Gly Tyr Tyr Asn# 300- TTT AAT TTT TCC TTT CTG AGT AGT TTT GTT TA - #T AAG GAG TCT AAT TTT 960Phe Asn Phe Ser Phe Leu Ser Ser Phe Val Ty - #r Lys Glu Ser Asn Phe305 3 - #10 3 - #15 3 -#20- ATG TAT GGA TCT TAT CAC CCA AGT TGT AAT TT - #T AGA CTA GAA ACT ATT1008Met Tyr Gly Ser Tyr His Pro Ser Cys Asn Ph - #e Arg Leu Glu Thr Ile# 335- AAT AAT GGC TTG TGG TTT AAT TCA CTT TCA GT - #T TCA ATT GCT TAC GGT1056Asn Asn Gly Leu Trp Phe Asn Ser Leu Ser Va - #l Ser Ile Ala Tyr Gly# 350- CCT CTT CAA GGT GGT TGC AAG CAA TCT GTC TT - #T AGT GGT AGA GCA ACT1104Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Ph - #e Ser Gly Arg Ala Thr# 365- TGT TGT TAT GCT TAT TCA TAT GGA GGT CCT TC - #G CTG TGT AAA GGT GTT1152Cys Cys Tyr Ala Tyr Ser Tyr Gly Gly Pro Se - #r Leu Cys Lys Gly Val# 380- TAT TCA GGT GAG TTA GAT CTT AAT TTT GAA TG - #T GGA CTG TTA GTT TAT1200Tyr Ser Gly Glu Leu Asp Leu Asn Phe Glu Cy - #s Gly Leu Leu Val Tyr385 3 - #90 3 - #95 4 -#00- GTT ACT AAG AGC GGT GGC TCT CGT ATA CAA AC - #A GCC ACT GAA CCG CCA1248Val Thr Lys Ser Gly Gly Ser Arg Ile Gln Th - #r Ala Thr Glu Pro Pro# 415- GTT ATA ACT CGA CAC AAT TAT AAT AAT ATT AC - #T TTA AAT ACT TGT GTT1296Val Ile Thr Arg His Asn Tyr Asn Asn Ile Th - #r Leu Asn Thr Cys Val# 430- GAT TAT AAT ATA TAT GGC AGA ACT GGC CAA GG - #T TTT ATT ACT AAT GTA1344Asp Tyr Asn Ile Tyr Gly Arg Thr Gly Gln Gl - #y Phe Ile Thr Asn Val# 445- ACC GAC TCA GCT GTT AGT TAT AAT TAT CTA GC - #A GAC GCA GGT TTG GCT1392Thr Asp Ser Ala Val Ser Tyr Asn Tyr Leu Al - #a Asp Ala Gly Leu Ala# 460- ATT TTA GAT ACA TCT GGT TCC ATA GAC ATC TT - #T GTT GTA CAA GGT GAA1440Ile Leu Asp Thr Ser Gly Ser Ile Asp Ile Ph - #e Val Val Gln Gly Glu465 4 - #70 4 - #75 4 -#80- TAT GGT CTT ACT TAT TAT AAG GTT AAC CCT TG - #C GAA GAT GTC AAC CAG1488Tyr Gly Leu Thr Tyr Tyr Lys Val Asn Pro Cy - #s Glu Asp Val Asn Gln# 495- CAG TTT GTA GTT TCT GGT GGT AAA TTA GTA GG - #T ATT CTT ACT TCA CGT1536Gln Phe Val Val Ser Gly Gly Lys Leu Val Gl - #y Ile Leu Thr Ser Arg# 510- AAT GAG ACT GGT TCT CAG CTT CTT GAG AAC CA - #G TTT TAC ATT AAA ATC1584Asn Glu Thr Gly Ser Gln Leu Leu Glu Asn Gl - #n Phe Tyr Ile Lys Ile# 525- ACT AAT GGA ACA CGT CGT TTT AGA CGT TCT AT - #T ACT GAA AAT GTT GCA1632Thr Asn Gly Thr Arg Arg Phe Arg Arg Ser Il - #e Thr Glu Asn Val Ala# 540- AAT TGC CCT TAT GTT AGT TAT GGT AAG TTT TG - #T ATA AAA CCT GAT GGT1680Asn Cys Pro Tyr Val Ser Tyr Gly Lys Phe Cy - #s Ile Lys Pro Asp Gly545 5 - #50 5 - #55 5 -#60- TCA ATT GCC ACA ATA GTA CCA AAA CAA TTG GA - #A CAG TTT GTG GCA CCT1728Ser Ile Ala Thr Ile Val Pro Lys Gln Leu Gl - #u Gln Phe Val Ala Pro# 575- TTA CTT AAT GTT ACT GAA AAT GTG CTC ATA CC - #T AAC AGT TTT AAT TTA1776Leu Leu Asn Val Thr Glu Asn Val Leu Ile Pr - #o Asn Ser Phe Asn Leu# 590- ACT GTT ACA GAT GAG TAC ATA CAA ACG CGT AT - #G GAT AAG GTC CAA ATT1824Thr Val Thr Asp Glu Tyr Ile Gln Thr Arg Me - #t Asp Lys Val Gln Ile# 605- AAT TGT CTG CAG TAT GTT TGT GGC AAT TCT CT - #G GAT TGT AGA GAT TTG1872Asn Cys Leu Gln Tyr Val Cys Gly Asn Ser Le - #u Asp Cys Arg Asp Leu# 620- TTT CAA CAA TAT GGG CCT GTT TGT GAC AAC AT - #A TTG TCT GTA GTA AAT1920Phe Gln Gln Tyr Gly Pro Val Cys Asp Asn Il - #e Leu Ser Val Val Asn625 6 - #30 6 - #35 6 -#40- AGT ATT GGT CAA AAA GAA GAT ATG GAA CTT TT - #G AAT TTC TAT TCT TCT1968Ser Ile Gly Gln Lys Glu Asp Met Glu Leu Le - #u Asn Phe Tyr Ser Ser# 655- ACT AAA CCG GCT GGT TTT AAT ACA CCA TTT CT - #T AGT AAT GTT AGC ACT2016Thr Lys Pro Ala Gly Phe Asn Thr Pro Phe Le - #u Ser Asn Val Ser Thr# 670- GGT GAG TTT AAT ATT TCT CTT CTG TTA ACA AC - #T CCT AGT AGT CCT AGA2064Gly Glu Phe Asn Ile Ser Leu Leu Leu Thr Th - #r Pro Ser Ser Pro Arg# 685- AGG CGT TCT TTT ATT GAA GAC CTT CTA TTT AC - #A AGC GTT GAA TCT GTT2112Arg Arg Ser Phe Ile Glu Asp Leu Leu Phe Th - #r Ser Val Glu Ser Val# 700- GGA TTA CCA ACA GAT GAC GCA TAC AAA AAT TG - #C ACT GCA GGA CCT TTA2160Gly Leu Pro Thr Asp Asp Ala Tyr Lys Asn Cy - #s Thr Ala Gly Pro Leu705 7 - #10 7 - #15 7 -#20- GGT TTT CTT AAG GAC CTT GCG TGT GCT CGT GA - #A TAT AAT GGT TTG CTT2208Gly Phe Leu Lys Asp Leu Ala Cys Ala Arg Gl - #u Tyr Asn Gly Leu Leu# 735- GTG TTG CCT CCC ATT ATA ACA GCA GAA ATG CA - #A ACT TTG TAT ACT AGT2256Val Leu Pro Pro Ile Ile Thr Ala Glu Met Gl - #n Thr Leu Tyr Thr Ser# 750- TCT CTA GTA GCT TCT ATG GCT TTT GGT GGT AT - #T ACT GCA GCT GGT GCT2304Ser Leu Val Ala Ser Met Ala Phe Gly Gly Il - #e Thr Ala Ala Gly Ala# 765- ATA CCT TTT GCC ACA CAA CTG CAG GCT AGA AT - #T AAT CAC TTG GGT ATT2352Ile Pro Phe Ala Thr Gln Leu Gln Ala Arg Il - #e Asn His Leu Gly Ile# 780- ACC CAG TCA CTT TTG TTG AAG AAT CAA GAA AA - #A ATT GCT GCT TCC TTT2400Thr Gln Ser Leu Leu Leu Lys Asn Gln Glu Ly - #s Ile Ala Ala Ser Phe785 7 - #90 7 - #95 8 -#00- AAT AAG GCC ATT GGT CGT ATG CAG GAA GGT TT - #T AGA AGT ACA TCT CTA2448Asn Lys Ala Ile Gly Arg Met Gln Glu Gly Ph - #e Arg Ser Thr Ser Leu# 815- GCA TTA CAA CAA ATT CAA GAT GTT GTT AAT AA - #G CAG AGT GCT ATT CTT2496Ala Leu Gln Gln Ile Gln Asp Val Val Asn Ly - #s Gln Ser Ala Ile Leu# 830- ACT GAG ACT ATG GCA TCA CTT AAT AAA AAT TT - #T GGT GCT ATT TCT TCT2544Thr Glu Thr Met Ala Ser Leu Asn Lys Asn Ph - #e Gly Ala Ile Ser Ser# 845#GCC ATA CAA GCA AAT GCT 2592AA CTT GACVal Ile Gln Glu Ile Tyr Gln Gln Leu Asp Al - #a Ile Gln Ala Asn Ala# 860- CAA GTG GAT CGT CTT ATA ACT GGT AGA TTG TC - #A TCA CTT TCT GTT TTA2640Gln Val Asp Arg Leu Ile Thr Gly Arg Leu Se - #r Ser Leu Ser Val Leu865 8 - #70 8 - #75 8 -#80- GCA TCT GCT AAG CAG GCG GAG CAT ATT AGA GT - #G TCA CAA CAG CGT GAG2688Ala Ser Ala Lys Gln Ala Glu His Ile Arg Va - #l Ser Gln Gln Arg Glu# 895- TTA GCT ACT CAG AAA ATT AAT GAG TGT GTT AA - #G TCA CAG TCT ATT AGG2736Leu Ala Thr Gln Lys Ile Asn Glu Cys Val Ly - #s Ser Gln Ser Ile Arg# 910- TAC TCC TTT TGT GGT AAT GGA CGA CAT GTT CT - #A ACC ATA CCG CAA AAT2784Tyr Ser Phe Cys Gly Asn Gly Arg His Val Le - #u Thr Ile Pro Gln Asn# 925- GCA CCT AAT GGT ATA GTG TTT ATA CAC TTT TC - #T TAT ACT CCA GAT AGT2832Ala Pro Asn Gly Ile Val Phe Ile His Phe Se - #r Tyr Thr Pro Asp Ser# 940- TTT GTT AAT GTT ACT GCA ATA GTG GGT TTT TG - #T GTA AAG CCA GCT AAT2880Phe Val Asn Val Thr Ala Ile Val Gly Phe Cy - #s Val Lys Pro Ala Asn945 9 - #50 9 - #55 9 -#60- GCT AGT CAG TAT GCA ATA GTA CCC GCT AAT GG - #T AGG GGT ATT TTT ATA2928#Gly Arg Gly Ile Phe Ile Val Pro Ala Asn# 975- CAA GTT AAT GGT AGT TAC TAC ATC ACA GCA CG - #A GAT ATG TAT ATG CCA2976Gln Val Asn Gly Ser Tyr Tyr Ile Thr Ala Ar - #g Asp Met Tyr Met Pro# 990- AGA GCT ATT ACT GCA GGA GAT ATA GTT ACG CT - #T ACT TCT TGT CAA GCA3024Arg Ala Ile Thr Ala Gly Asp Ile Val Thr Le - #u Thr Ser Cys Gln Ala# 10050- AAT TAT GTA AGT GTA AAT AAG ACC GTC ATT AC - #T ACA TTC GTA GAC AAT3072Asn Tyr Val Ser Val Asn Lys Thr Val Ile Th - #r Thr Phe Val Asp Asn# 10205- GAT GAT TTT GAT TTT AAT GAC GAA TTG TCA AA - #A TGG TGG AAT GAC ACT3120Asp Asp Phe Asp Phe Asn Asp Glu Leu Ser Ly - #s Trp Trp Asn Asp Thr# 10401030 - # 1035- AAG CAT GAG CTA CCA GAC TTT GAC AAA TTC AA - #T TAC ACA GTA CCT ATA3168Lys His Glu Leu Pro Asp Phe Asp Lys Phe As - #n Tyr Thr Val Pro Ile# 10550- CTT GAC ATT GAT AGT GAA ATT GAT CGT ATT CA - #A GGC GTT ATA CAG GGT3216Leu Asp Ile Asp Ser Glu Ile Asp Arg Ile Gl - #n Gly Val Ile Gln Gly# 10705- CTT AAT GAC TCT TTA ATA GAC CTT GAA AAA CT - #T TCA ATA CTC AAA ACT3264Leu Asn Asp Ser Leu Ile Asp Leu Glu Lys Le - #u Ser Ile Leu Lys Thr# 10850- TAT ATT AAG TGG CCT TGG TAT GTG TGG TTA GC - #C ATA GCT TTT GCC ACT3312Tyr Ile Lys Trp Pro Trp Tyr Val Trp Leu Al - #a Ile Ala Phe Ala Thr# 11005- ATT ATC TTC ATC TTA ATA CTA GGA TGG GTT TT - #C TTC ATG ACT GGA TGT3360Ile Ile Phe Ile Leu Ile Leu Gly Trp Val Ph - #e Phe Met Thr Gly Cys# 11201110 - # 1115- TGT GGT TGT TGT TGT GGA TGC TTT GGC ATT AT - #G CCT CTA ATG AGT AAG3408Cys Gly Cys Cys Cys Gly Cys Phe Gly Ile Me - #t Pro Leu Met Ser Lys# 11350- TGT GGT AAG AAA TCT TCT TAT TAC ACG ACT TT - #T GAT AAC GAT GTG GTA3456Cys Gly Lys Lys Ser Ser Tyr Tyr Thr Thr Ph - #e Asp Asn Asp Val Val# 11505# 3489A AAC AGA CCT AAA AAG TCT GTT TA - #AThr Glu Gln Asn Arg Pro Lys Lys Ser Val# 1160- (2) INFORMATION FOR SEQ ID NO:15:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 1162 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:- Met Leu Val Thr Pro Leu Leu Leu Val Thr Le - #u Leu Cys Val Leu Cys# 15- Ser Ala Ala Leu Tyr Asp Ser Ser Ser Tyr Va - #l Tyr Tyr Tyr Gln Ser# 30- Ala Phe Arg Pro Pro Asn Gly Trp His Leu Hi - #s Gly Gly Ala Tyr Ala# 45- Val Val Asn Ile Ser Ser Glu Ser Asn Asn Al - #a Gly Ser Ser Pro Gly# 60- Cys Ile Val Gly Thr Ile His Gly Gly Arg Va - #l Val Asn Ala Ser Ser# 80- Ile Ala Met Thr Ala Pro Ser Ser Gly Met Al - #a Trp Ser Ser Ser Gln# 95- Phe Cys Thr Ala His Cys Asn Phe Ser Asp Th - #r Thr Val Phe Val Thr# 110- His Cys Tyr Lys Tyr Asp Gly Cys Pro Ile Th - #r Gly Met Leu Gln Lys# 125- Asn Phe Leu Arg Val Ser Ala Met Lys Asn Gl - #y Gln Leu Phe Tyr Asn# 140- Leu Thr Val Ser Val Ala Lys Tyr Pro Thr Ph - #e Lys Ser Phe Gln Cys145 1 - #50 1 - #55 1 -#60- Val Asn Asn Leu Thr Ser Val Tyr Leu Asn Gl - #y Asp Leu Val Tyr Thr# 175- Ser Asn Glu Thr Thr Asp Val Thr Ser Ala Gl - #y Val Tyr Phe Lys Ala# 190- Gly Gly Pro Ile Thr Tyr Lys Val Met Arg Ly - #s Val Lys Ala Leu Ala# 205- Tyr Phe Val Asn Gly Thr Ala Gln Asp Val Il - #e Leu Cys Asp Gly Ser# 220- Pro Arg Gly Leu Leu Ala Cys Gln Tyr Asn Th - #r Gly Asn Phe Ser Asp225 2 - #30 2 - #35 2 -#40- Gly Phe Tyr Pro Phe Ile Asn Ser Ser Leu Va - #l Lys Gln Lys Phe Ile# 255- Val Tyr Arg Glu Asn Ser Val Asn Thr Thr Ph - #e Thr Leu His Asn Phe# 270- Thr Phe His Asn Glu Thr Gly Ala Asn Pro As - #n Pro Ser Gly Val Gln# 285- Asn Ile Leu Thr Tyr Gln Thr Gln Thr Ala Gl - #n Ser Gly Tyr Tyr Asn# 300- Phe Asn Phe Ser Phe Leu Ser Ser Phe Val Ty - #r Lys Glu Ser Asn Phe305 3 - #10 3 - #15 3 -#20- Met Tyr Gly Ser Tyr His Pro Ser Cys Asn Ph - #e Arg Leu Glu Thr Ile# 335- Asn Asn Gly Leu Trp Phe Asn Ser Leu Ser Va - #l Ser Ile Ala Tyr Gly# 350- Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Ph - #e Ser Gly Arg Ala Thr# 365- Cys Cys Tyr Ala Tyr Ser Tyr Gly Gly Pro Se - #r Leu Cys Lys Gly Val# 380- Tyr Ser Gly Glu Leu Asp Leu Asn Phe Glu Cy - #s Gly Leu Leu Val Tyr385 3 - #90 3 - #95 4 -#00- Val Thr Lys Ser Gly Gly Ser Arg Ile Gln Th - #r Ala Thr Glu Pro Pro# 415- Val Ile Thr Arg His Asn Tyr Asn Asn Ile Th - #r Leu Asn Thr Cys Val# 430- Asp Tyr Asn Ile Tyr Gly Arg Thr Gly Gln Gl - #y Phe Ile Thr Asn Val# 445- Thr Asp Ser Ala Val Ser Tyr Asn Tyr Leu Al - #a Asp Ala Gly Leu Ala# 460- Ile Leu Asp Thr Ser Gly Ser Ile Asp Ile Ph - #e Val Val Gln Gly Glu465 4 - #70 4 - #75 4 -#80- Tyr Gly Leu Thr Tyr Tyr Lys Val Asn Pro Cy - #s Glu Asp Val Asn Gln# 495- Gln Phe Val Val Ser Gly Gly Lys Leu Val Gl - #y Ile Leu Thr Ser Arg# 510- Asn Glu Thr Gly Ser Gln Leu Leu Glu Asn Gl - #n Phe Tyr Ile Lys Ile# 525- Thr Asn Gly Thr Arg Arg Phe Arg Arg Ser Il - #e Thr Glu Asn Val Ala# 540- Asn Cys Pro Tyr Val Ser Tyr Gly Lys Phe Cy - #s Ile Lys Pro Asp Gly545 5 - #50 5 - #55 5 -#60- Ser Ile Ala Thr Ile Val Pro Lys Gln Leu Gl - #u Gln Phe Val Ala Pro# 575- Leu Leu Asn Val Thr Glu Asn Val Leu Ile Pr - #o Asn Ser Phe Asn Leu# 590- Thr Val Thr Asp Glu Tyr Ile Gln Thr Arg Me - #t Asp Lys Val Gln Ile# 605- Asn Cys Leu Gln Tyr Val Cys Gly Asn Ser Le - #u Asp Cys Arg Asp Leu# 620- Phe Gln Gln Tyr Gly Pro Val Cys Asp Asn Il - #e Leu Ser Val Val Asn625 6 - #30 6 - #35 6 -#40- Ser Ile Gly Gln Lys Glu Asp Met Glu Leu Le - #u Asn Phe Tyr Ser Ser# 655- Thr Lys Pro Ala Gly Phe Asn Thr Pro Phe Le - #u Ser Asn Val Ser Thr# 670- Gly Glu Phe Asn Ile Ser Leu Leu Leu Thr Th - #r Pro Ser Ser Pro Arg# 685- Arg Arg Ser Phe Ile Glu Asp Leu Leu Phe Th - #r Ser Val Glu Ser Val# 700- Gly Leu Pro Thr Asp Asp Ala Tyr Lys Asn Cy - #s Thr Ala Gly Pro Leu705 7 - #10 7 - #15 7 -#20- Gly Phe Leu Lys Asp Leu Ala Cys Ala Arg Gl - #u Tyr Asn Gly Leu Leu# 735- Val Leu Pro Pro Ile Ile Thr Ala Glu Met Gl - #n Thr Leu Tyr Thr Ser# 750- Ser Leu Val Ala Ser Met Ala Phe Gly Gly Il - #e Thr Ala Ala Gly Ala# 765- Ile Pro Phe Ala Thr Gln Leu Gln Ala Arg Il - #e Asn His Leu Gly Ile# 780- Thr Gln Ser Leu Leu Leu Lys Asn Gln Glu Ly - #s Ile Ala Ala Ser Phe785 7 - #90 7 - #95 8 -#00- Asn Lys Ala Ile Gly Arg Met Gln Glu Gly Ph - #e Arg Ser Thr Ser Leu# 815- Ala Leu Gln Gln Ile Gln Asp Val Val Asn Ly - #s Gln Ser Ala Ile Leu# 830- Thr Glu Thr Met Ala Ser Leu Asn Lys Asn Ph - #e Gly Ala Ile Ser Ser# 845- Val Ile Gln Glu Ile Tyr Gln Gln Leu Asp Al - #a Ile Gln Ala Asn Ala# 860- Gln Val Asp Arg Leu Ile Thr Gly Arg Leu Se - #r Ser Leu Ser Val Leu865 8 - #70 8 - #75 8 -#80- Ala Ser Ala Lys Gln Ala Glu His Ile Arg Va - #l Ser Gln Gln Arg Glu# 895- Leu Ala Thr Gln Lys Ile Asn Glu Cys Val Ly - #s Ser Gln Ser Ile Arg# 910- Tyr Ser Phe Cys Gly Asn Gly Arg His Val Le - #u Thr Ile Pro Gln Asn# 925- Ala Pro Asn Gly Ile Val Phe Ile His Phe Se - #r Tyr Thr Pro Asp Ser# 940- Phe Val Asn Val Thr Ala Ile Val Gly Phe Cy - #s Val Lys Pro Ala Asn945 9 - #50 9 - #55 9 -#60- Ala Ser Gln Tyr Ala Ile Val Pro Ala Asn Gl - #y Arg Gly Ile Phe Ile# 975- Gln Val Asn Gly Ser Tyr Tyr Ile Thr Ala Ar - #g Asp Met Tyr Met Pro# 990- Arg Ala Ile Thr Ala Gly Asp Ile Val Thr Le - #u Thr Ser Cys Gln Ala# 10050- Asn Tyr Val Ser Val Asn Lys Thr Val Ile Th - #r Thr Phe Val Asp Asn# 10205- Asp Asp Phe Asp Phe Asn Asp Glu Leu Ser Ly - #s Trp Trp Asn Asp Thr# 10401030 - # 1035- Lys His Glu Leu Pro Asp Phe Asp Lys Phe As - #n Tyr Thr Val Pro Ile# 10550- Leu Asp Ile Asp Ser Glu Ile Asp Arg Ile Gl - #n Gly Val Ile Gln Gly# 10705- Leu Asn Asp Ser Leu Ile Asp Leu Glu Lys Le - #u Ser Ile Leu Lys Thr# 10850- Tyr Ile Lys Trp Pro Trp Tyr Val Trp Leu Al - #a Ile Ala Phe Ala Thr# 11005- Ile Ile Phe Ile Leu Ile Leu Gly Trp Val Ph - #e Phe Met Thr Gly Cys#1110 111 - #5 1120- Cys Gly Cys Cys Cys Gly Cys Phe Gly Ile Me - #t Pro Leu Met Ser Lys# 11350- Cys Gly Lys Lys Ser Ser Tyr Tyr Thr Thr Ph - #e Asp Asn Asp Val Val# 11505- Thr Glu Gln Asn Arg Pro Lys Lys Ser Val# 1160- (2) INFORMATION FOR SEQ ID NO:16:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1846 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..1846- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:- ATG TTG GTG AAG TCA CTG TTT CTA GTG ACC AT - #T TTG TTT GCA CTA TGT 48Met Leu Val Lys Ser Leu Phe Leu Val Thr Il - #e Leu Phe Ala Leu Cys# 15- AGT GCT AAT TTA TAT GAC AAC GAA TCT TTT GT - #G TAT TAC TAC CAG AGT 96Ser Ala Asn Leu Tyr Asp Asn Glu Ser Phe Va - #l Tyr Tyr Tyr Gln Ser# 30- GCT TTT AGG CCA GGA CAT GGT TGG CAT TTA CA - #T GGA GGT GCT TAT GCA 144Ala Phe Arg Pro Gly His Gly Trp His Leu Hi - #s Gly Gly Ala Tyr Ala# 45- GTA GTT AAT GTG TCT AGT GAA AAT AAT AAT GC - #A GGT ACT GCC CCA AGT 192Val Val Asn Val Ser Ser Glu Asn Asn Asn Al - #a Gly Thr Ala Pro Ser# 60- TGC ACT GCT GGT GCT ATT GGC TAC AGT AAG AA - #T TTC AGT GCG GCC TCA 240Cys Thr Ala Gly Ala Ile Gly Tyr Ser Lys As - #n Phe Ser Ala Ala Ser# 80- GTA GCC ATG ACT GCA CCA CTA AGT GGT ATG TC - #A TGG TCT GCC TCA TCT 288Val Ala Met Thr Ala Pro Leu Ser Gly Met Se - #r Trp Ser Ala Ser Ser# 95- TTT TGT ACA GCT CAC TGT AAT TTT ACT TCT TA - #T ATA GTG TTT GTT ACA 336Phe Cys Thr Ala His Cys Asn Phe Thr Ser Ty - #r Ile Val Phe Val Thr# 110- CAT TGT TTT AAG AGC GGA TCT AAT AGT TGT CC - #T TTG ACA GGT CTT ATT 384His Cys Phe Lys Ser Gly Ser Asn Ser Cys Pr - #o Leu Thr Gly Leu Ile# 125- CCA AGC GGT TAT ATT CGT ATT GCT GCT ATG AA - #A CAT GGA AGT CGT ACG 432Pro Ser Gly Tyr Ile Arg Ile Ala Ala Met Ly - #s His Gly Ser Arg Thr# 140- CCT GGT CAC TTA TTT TAT AAC TTA ACA GTT TC - #T GTG ACT AAA TAT CCT 480Pro Gly His Leu Phe Tyr Asn Leu Thr Val Se - #r Val Thr Lys Tyr Pro145 1 - #50 1 - #55 1 -#60- AAG TTT AGA TCG CTA CAA TGT GTT AAT AAT CA - #T ACT TCT GTA TAT TTA 528Lys Phe Arg Ser Leu Gln Cys Val Asn Asn Hi - #s Thr Ser Val Tyr Leu# 175- AAT GGT GAC CTT GTT TTC ACA TCT AAC TAT AC - #T GAA GAT GTT GTA GCT 576Asn Gly Asp Leu Val Phe Thr Ser Asn Tyr Th - #r Glu Asp Val Val Ala# 190- GCA GGT GTC CAT TTT AAA AGT GGT GGA CCT AT - #A ACT TAT AAA GTT ATG 624Ala Gly Val His Phe Lys Ser Gly Gly Pro Il - #e Thr Tyr Lys Val Met# 205- AGA GAG GTT AAA GCC TTG GCT TAT TTT GTC AA - #T GGT ACT GCA CAT GAT 672Arg Glu Val Lys Ala Leu Ala Tyr Phe Val As - #n Gly Thr Ala His Asp# 220- GTC ATT CTA TGT GAT GAC ACA CCT AGA GGT TT - #G TTA GCA TGC CAA TAT 720Val Ile Leu Cys Asp Asp Thr Pro Arg Gly Le - #u Leu Ala Cys Gln Tyr225 2 - #30 2 - #35 2 -#40- AAT ACT GGC AAT TTT TCA GAT GGC TTC TAT CC - #T TTT ACT AAT ACT AGT 768Asn Thr Gly Asn Phe Ser Asp Gly Phe Tyr Pr - #o Phe Thr Asn Thr Ser# 255- ATT GTT AAG GAT AAG TTT ATT GTT TAT CGT GA - #A AGT AGT GTC AAT ACT 816Ile Val Lys Asp Lys Phe Ile Val Tyr Arg Gl - #u Ser Ser Val Asn Thr# 270- ACT TTG ACA TTA ACT AAT TTC ACG TTT AGT AA - #T GAA AGT GGT GCC CCT 864Thr Leu Thr Leu Thr Asn Phe Thr Phe Ser As - #n Glu Ser Gly Ala Pro# 285- CCT AAT ACA GGT GGT GTT GAC AGT TTT ATT TT - #A TAC CAG ACA CAA ACA 912Pro Asn Thr Gly Gly Val Asp Ser Phe Ile Le - #u Tyr Gln Thr Gln Thr# 300- GCT CAG AGT GGT TAT TAT AAT TTT AAT TTT TC - #A TTT CTG AGT AGT TTT 960Ala Gln Ser Gly Tyr Tyr Asn Phe Asn Phe Se - #r Phe Leu Ser Ser Phe305 3 - #10 3 - #15 3 -#20- GTT TAT AGG GAA AGT AAT TAT ATG TAT GGA TC - #T TAC CAT CCG GCT TGT1008Val Tyr Arg Glu Ser Asn Tyr Met Tyr Gly Se - #r Tyr His Pro Ala Cys# 335- AGT TTT AGA CCT GAA ACC CTT AAT GGT TTG TG - #G TCT AAT TCC CTT TCT1056Ser Phe Arg Pro Glu Thr Leu Asn Gly Leu Tr - #p Ser Asn Ser Leu Ser# 350- GTT TCA TTA ATA TAC GGT CCC ATT CAA GGT GG - #T TGT AAG CAA TCT GTA1104Val Ser Leu Ile Tyr Gly Pro Ile Gln Gly Gl - #y Cys Lys Gln Ser Val# 365- TTT AAT GGT AAA GCA ACT TGT TGT TAT GCT TA - #T TCA TAC GGA GGA CCT1152Phe Asn Gly Lys Ala Thr Cys Cys Tyr Ala Ty - #r Ser Tyr Gly Gly Pro# 380- CGT GCT TGT AAA GGT GTC TAT AGA GGT GAG CT - #A ACA CAG CAT TTT GAA1200Arg Ala Cys Lys Gly Val Tyr Arg Gly Glu Le - #u Thr Gln His Phe Glu385 3 - #90 3 - #95 4 -#00- TGT GGT TTG TTA GTT TAT GTT ACT AAG AGC GA - #T GGC TCC CGT ATA CAA1248Cys Gly Leu Leu Val Tyr Val Thr Lys Ser As - #p Gly Ser Arg Ile Gln# 415- ACT GCA ACA CAA CCA CCT GTA TTA ACC CAA AA - #T TTT TAT AAT AAC ATC1296Thr Ala Thr Gln Pro Pro Val Leu Thr Gln As - #n Phe Tyr Asn Asn Ile# 430- ACT TTA GGT AAG TGT GTT GAT TAT AAT GTT TA - #T GGT AGA ACT GGA CAA1344Thr Leu Gly Lys Cys Val Asp Tyr Asn Val Ty - #r Gly Arg Thr Gly Gln# 445- GGT TTT ATT ACT AAT GTA ACT GAT TTA GCT AC - #T TCC CAT AAT TAC TTA1392Gly Phe Ile Thr Asn Val Thr Asp Leu Ala Th - #r Ser His Asn Tyr Leu# 460- GCG GAG GGA GGA TTA GCT ATT TTA GAT ACA TC - #T GGT GCC ATA GAC ATC1440Ala Glu Gly Gly Leu Ala Ile Leu Asp Thr Se - #r Gly Ala Ile Asp Ile465 4 - #70 4 - #75 4 -#80- TTC GTT GTA CAA GGT GAA TAT GGC CCT AAC TA - #C TAT AAG GTT AAT CTA1488Phe Val Val Gln Gly Glu Tyr Gly Pro Asn Ty - #r Tyr Lys Val Asn Leu# 495- TGT GAA GAT GTT AAC CAA CAG TTT GTA GTT TC - #T GGT GGT AAA TTA GTA1536Cys Glu Asp Val Asn Gln Gln Phe Val Val Se - #r Gly Gly Lys Leu Val# 510#TCT CAG CCT CTT GAA AAC 1584AA ACT GGTGly Ile Leu Thr Ser Arg Asn Glu Thr Gly Se - #r Gln Pro Leu Glu Asn# 525- CAG TTT TAC ATT AAG ATC ACT AAT GGA ACA CA - #T CGT TCT AGA CGT TCT1632Gln Phe Tyr Ile Lys Ile Thr Asn Gly Thr Hi - #s Arg Ser Arg Arg Ser# 540- GTT AAT GAA AAT GTT ACG AAT TGC CCT TAT GT - #T AGT TAT GGC AAG TTT1680Val Asn Glu Asn Val Thr Asn Cys Pro Tyr Va - #l Ser Tyr Gly Lys Phe545 5 - #50 5 - #55 5 -#60- TGT ATA AAA CCT GAT GGT TCA GTT TCT CCT AT - #A GTA CCA AAA GAA CTT1728Cys Ile Lys Pro Asp Gly Ser Val Ser Pro Il - #e Val Pro Lys Glu Leu# 575- GAA CAG TTT GTG GCA CCT TTA CTT AAT GTT AC - #T GAA AAT GTG CTC ATA1776Glu Gln Phe Val Ala Pro Leu Leu Asn Val Th - #r Glu Asn Val Leu Ile# 590- CCT AAC AGT TTT AAC TTA ACT GTT ACA GAT GA - #G TAC ATA CAA ACG CGT1824Pro Asn Ser Phe Asn Leu Thr Val Thr Asp Gl - #u Tyr Ile Gln Thr Arg# 605# 1846A ATT AGG AMet Asp Lys Val Gln Ile Arg# 615- (2) INFORMATION FOR SEQ ID NO:17:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 615 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:- Met Leu Val Lys Ser Leu Phe Leu Val Thr Il - #e Leu Phe Ala Leu Cys# 15- Ser Ala Asn Leu Tyr Asp Asn Glu Ser Phe Va - #l Tyr Tyr Tyr Gln Ser# 30- Ala Phe Arg Pro Gly His Gly Trp His Leu Hi - #s Gly Gly Ala Tyr Ala# 45- Val Val Asn Val Ser Ser Glu Asn Asn Asn Al - #a Gly Thr Ala Pro Ser# 60- Cys Thr Ala Gly Ala Ile Gly Tyr Ser Lys As - #n Phe Ser Ala Ala Ser# 80- Val Ala Met Thr Ala Pro Leu Ser Gly Met Se - #r Trp Ser Ala Ser Ser# 95- Phe Cys Thr Ala His Cys Asn Phe Thr Ser Ty - #r Ile Val Phe Val Thr# 110- His Cys Phe Lys Ser Gly Ser Asn Ser Cys Pr - #o Leu Thr Gly Leu Ile# 125- Pro Ser Gly Tyr Ile Arg Ile Ala Ala Met Ly - #s His Gly Ser Arg Thr# 140- Pro Gly His Leu Phe Tyr Asn Leu Thr Val Se - #r Val Thr Lys Tyr Pro145 1 - #50 1 - #55 1 -#60- Lys Phe Arg Ser Leu Gln Cys Val Asn Asn Hi - #s Thr Ser Val Tyr Leu# 175- Asn Gly Asp Leu Val Phe Thr Ser Asn Tyr Th - #r Glu Asp Val Val Ala# 190- Ala Gly Val His Phe Lys Ser Gly Gly Pro Il - #e Thr Tyr Lys Val Met# 205- Arg Glu Val Lys Ala Leu Ala Tyr Phe Val As - #n Gly Thr Ala His Asp# 220- Val Ile Leu Cys Asp Asp Thr Pro Arg Gly Le - #u Leu Ala Cys Gln Tyr#240- Asn Thr Gly Asn Phe Ser Asp Gly Phe Tyr Pr - #o Phe Thr Asn Thr Ser# 255- Ile Val Lys Asp Lys Phe Ile Val Tyr Arg Gl - #u Ser Ser Val Asn Thr# 270- Thr Leu Thr Leu Thr Asn Phe Thr Phe Ser As - #n Glu Ser Gly Ala Pro# 285- Pro Asn Thr Gly Gly Val Asp Ser Phe Ile Le - #u Tyr Gln Thr Gln Thr# 300- Ala Gln Ser Gly Tyr Tyr Asn Phe Asn Phe Se - #r Phe Leu Ser Ser Phe305 3 - #10 3 - #15 3 -#20- Val Tyr Arg Glu Ser Asn Tyr Met Tyr Gly Se - #r Tyr His Pro Ala Cys# 335- Ser Phe Arg Pro Glu Thr Leu Asn Gly Leu Tr - #p Ser Asn Ser Leu Ser# 350- Val Ser Leu Ile Tyr Gly Pro Ile Gln Gly Gl - #y Cys Lys Gln Ser Val# 365- Phe Asn Gly Lys Ala Thr Cys Cys Tyr Ala Ty - #r Ser Tyr Gly Gly Pro# 380- Arg Ala Cys Lys Gly Val Tyr Arg Gly Glu Le - #u Thr Gln His Phe Glu385 3 - #90 3 - #95 4 -#00- Cys Gly Leu Leu Val Tyr Val Thr Lys Ser As - #p Gly Ser Arg Ile Gln# 415- Thr Ala Thr Gln Pro Pro Val Leu Thr Gln As - #n Phe Tyr Asn Asn Ile# 430- Thr Leu Gly Lys Cys Val Asp Tyr Asn Val Ty - #r Gly Arg Thr Gly Gln# 445- Gly Phe Ile Thr Asn Val Thr Asp Leu Ala Th - #r Ser His Asn Tyr Leu# 460- Ala Glu Gly Gly Leu Ala Ile Leu Asp Thr Se - #r Gly Ala Ile Asp Ile465 4 - #70 4 - #75 4 -#80- Phe Val Val Gln Gly Glu Tyr Gly Pro Asn Ty - #r Tyr Lys Val Asn Leu# 495- Cys Glu Asp Val Asn Gln Gln Phe Val Val Se - #r Gly Gly Lys Leu Val# 510- Gly Ile Leu Thr Ser Arg Asn Glu Thr Gly Se - #r Gln Pro Leu Glu Asn# 525- Gln Phe Tyr Ile Lys Ile Thr Asn Gly Thr Hi - #s Arg Ser Arg Arg Ser# 540- Val Asn Glu Asn Val Thr Asn Cys Pro Tyr Va - #l Ser Tyr Gly Lys Phe545 5 - #50 5 - #55 5 -#60- Cys Ile Lys Pro Asp Gly Ser Val Ser Pro Il - #e Val Pro Lys Glu Leu# 575- Glu Gln Phe Val Ala Pro Leu Leu Asn Val Th - #r Glu Asn Val Leu Ile# 590- Pro Asn Ser Phe Asn Leu Thr Val Thr Asp Gl - #u Tyr Ile Gln Thr Arg# 605- Met Asp Lys Val Gln Ile Arg# 615- (2) INFORMATION FOR SEQ ID NO:18:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 2116 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:- TATAATTATC TAGCAGACGC AGGTATGGCT ATTTTAGATA CATCTGGTTC CA - #TAGACATC 60- TTTGTTGCAC AAGGTGAATA TGGCCTTACT TATTATAAGG CTAACCCTTG CG - #AAGACGTC 120- AACCAGCAGT TTGTAGTTTC TGGTGGTAAA TTAGTAGGTA TTCTTACTTC AC - #GTAATGAG 180- ACTGGTTCTC AGCTTCTTGA GAACCAGTTT TACATTAAAA TCACTAATGG AA - #CACGTCGT 240- TCTAGACGTT CTATTACTGC AAATGTHACA AATYGCCCTT ATGTTAGCTA TG - #GCAAGTTT 300- TGTCTAAAAC CTGATGGYTC AGYTTCTGYT ATAGCACCAC NNNNNNNNNN NN - #NNNNNNNN 360- NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NN - #NNNNNNNN 420- NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NN - #NNNNNNNT 480- GTTTGTGGCA ATTCTCTGGA TTGTAGAAAG TTGYTTCAAC AATATGGGCC TG - #TTTGBGAC 540- AACATATTGT CTGTGGTAAA TAGTGTTGGT CAAAAAGAAG ATATGGAACT TC - #UAAATCTC 600- TATTCTTCTA CTAAACCATC TGGCTTTAAT ACACCAGTTT TTAGTAATCT YA - #GCACTGGC 660- GATTTYAATA TTTCTCTTYT GGTTGACACC TCCAGTAGTA CTACTGGGCG CT - #CTTTTATT 720- GAAGATCTTT TATTTACAAG TGTTGAATCT GTTGGATTAC CAACAGATGA AG - #CTTATAAA 780- AAGTGCACTG CAGGACCTTT AGGCTTCCTT AAGGACCTBG CGTGTGCTCG TG - #AATATAAT 840- GGCTTGCTTG YNNNNNNCCC TATTATAACA GCAGAAATGC AAACCTTGTA TA - #CTAGTTCT 900- TTAGTAGCTT CTATGGCTTT TGGTGGGATT ACTGCAGCTG GTGCTATACC TT - #TTGCCACA 960- CAACTGCAGG CTAGAATTAA TCACTTGGGT ATTACCCAGT CACTTTTGCA GA - #AAAATCAA1020- GAAAAAATTG CTGCCTCCTT TAATAAGGCC ATTGGCCATA TGCAGGAAGG TT - #TTAGAAGT1080- ACATCTCTAG CATTACAACA AGTYCAMGAT GTTGTTAATA AGCAGAGTGC TA - #TTCTTACT1140- GAGACTATGG CATCACTTAA TAAAAATTTK GGTGCTATTT CTTCTGTGAT TC - #AAGATATC1200- TACCAGCAAC TTGACGCCAT ACAAGCAAAT GCTCAAGTGG ATCGTCTTAT AA - #CTGGTAGA1260- TTGTCATCAC TTTCTGTTTT AGCATCTGCT AAGCAGGCGG AGTATATTAG AG - #TGTCACAA1320- CAGCGTGAGT TAGCTACTCA GAAAATTAAT GAGTGTGTTA AATCACAGTC TA - #TTAGGTAC1380- TCCTTTTGTG GTAATGGACG ACACGTTCTA ACTATACCGC AAAATGCACC TA - #ATGGTATA1440- GTGTTTATAC ACTTTACTTA TACTCCAGAG AGTTTTGKTA ATGTTACTGC AA - #TAGTGGGT1500- TTTTGTAARG CCGCTAATGC TAGTCAGTAT GCAATAGTGC CTGCTAATGG CA - #GAGGTATT1560- TCTATACAAG TTAATGGTAG TCACTACATC ACTGCACGAG ATATGTATAT GC - #CAAGAGAT1620- ATTACTGCAG GAGATATAGT TACGCTTACT TCTTGTCAAG CAAATTATGT AA - #GTGTAMMT1680- AAGACCGTCA TTACYACATT HGTAGACAAT GATGATTTTG ATTTTGATGA CG - #AATTGTCA1740- AAATGGTGGA ATGATACTAA GCATGAGCTA CCAGACTTTG ACGAATTCAA TT - #ACACAGTA1800- CCTATACTTG ACATTGGTAG TGAAATTGAT CGTATTCAAG GCGTTATACA GG - #GCCTTAAT1860- GACTCTCTAA TAGACCTTGA AACACTATCA ATACTCAAAA CTTATATTAA GT - #GGCCTTGG1920- TATGTGTGGT TAGCCATAGC TTTTGSCACT ATTATCTTCA TCCTAATATT AG - #GGTGGGTG1980- TTTTTCATGA CTGGTTGTTG TGGTTGTTGT TGTGGATGCT TTGGCATTAT TC - #CTCTAATG2040- AGCAAGTGTG GTAAGAAATC TTCTTATTAC ACGACTTTGG ATAATGATGT GG - #TAACTGAA2100# 2116- (2) INFORMATION FOR SEQ ID NO:19:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 705 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:- Tyr Asn Tyr Leu Ala Asp Ala Gly - # Met Ala Ile Leu Asp Thr SerGly# 15- Ser Ile Asp Ile Phe Val Ala Gln - # Gly Glu Tyr Gly Leu Thr TyrTyr# 30- Lys Ala Asn Pro Cys Glu Asp Val - # Asn Gln Gln Phe Val Val SerGly# 45- Gly Lys Leu Val Gly Ile Leu Thr - # Ser Arg Asn Glu Thr Gly SerGln# 60- Leu Leu Glu Asn Gln Phe Tyr Ile - # Lys Ile Thr Asn Gly Thr ArgArg# 80- Ser Arg Arg Ser Ile Thr Ala Asn - # Val Thr Asn Xaa Pro Tyr ValSer# 95- Tyr Gly Lys Phe Cys Leu Lys Pro - # Asp Gly Ser Xaa Ser Xaa IleAla# 110- Pro Xaa Xaa Xaa Xaa Xaa Xaa Xaa - # Xaa Xaa Xaa Xaa Xaa Xaa XaaXaa# 125#Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa# 140- Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa - # Xaa Xaa Xaa Xaa Xaa Xaa XaaXaa# 160- Val Cys Gly Asn Ser Leu Asp Cys - # Arg Lys Leu Xaa Gln Gln TyrGly# 175- Pro Val Xaa Asp Asn Ile Leu Ser - # Val Val Asn Ser Val Gly GlnLys# 190- Glu Asp Met Glu Leu Leu Asn Leu - # Tyr Ser Ser Thr Lys Pro SerGly# 205- Phe Asn Thr Pro Val Phe Ser Asn - # Leu Ser Thr Gly Asp Phe AsnIle# 220- Ser Leu Leu Val Asp Thr Ser Ser - # Ser Thr Thr Gly Arg Ser PheIle# 240- Glu Asp Leu Leu Phe Thr Ser Val - # Glu Ser Val Gly Leu Pro ThrAsp# 255- Glu Ala Tyr Lys Lys Cys Thr Ala - # Gly Pro Leu Gly Phe Leu LysAsp# 270- Leu Ala Cys Ala Arg Glu Tyr Asn - # Gly Leu Leu Xaa Xaa Xaa ProIle# 285- Ile Thr Ala Glu Met Gln Thr Leu - # Tyr Thr Ser Ser Leu Val AlaSer# 300- Met Ala Phe Gly Gly Ile Thr Ala - # Ala Gly Ala Ile Pro Phe AlaThr# 320- Gln Leu Gln Ala Arg Ile Asn His - # Leu Gly Ile Thr Gln Ser LeuLeu# 335- Gln Lys Asn Gln Glu Lys Ile Ala - # Ala Ser Phe Asn Lys Ala IleGly# 350- His Met Gln Glu Gly Phe Arg Ser - # Thr Ser Leu Ala Leu Gln GlnVal# 365- Xaa Asp Val Val Asn Lys Gln Ser - # Ala Ile Leu Thr Glu Thr MetAla# 380- Ser Leu Asn Lys Asn Xaa Gly Ala - # Ile Ser Ser Val Ile Gln AspIle# 400- Tyr Gln Gln Leu Asp Ala Ile Gln - # Ala Asn Ala Gln Val Asp ArgLeu# 415- Ile Thr Gly Arg Leu Ser Ser Leu - # Ser Val Leu Ala Ser Ala LysGln# 430- Ala Glu Tyr Ile Arg Val Ser Gln - # Gln Arg Glu Leu Ala Thr GlnLys# 445- Ile Asn Glu Cys Val Lys Ser Gln - # Ser Ile Arg Tyr Ser Phe CysGly# 460- Asn Gly Arg His Val Leu Thr Ile - # Pro Gln Asn Ala Pro Asn GlyIle# 480- Val Phe Ile His Phe Thr Tyr Thr - # Pro Glu Ser Phe Xaa Asn ValThr# 495- Ala Ile Val Gly Phe Cys Lys Ala - # Ala Asn Ala Ser Gln Tyr AlaIle# 510- Val Pro Ala Asn Gly Arg Gly Ile - # Ser Ile Gln Val Asn Gly SerHis# 525- Tyr Ile Thr Ala Arg Asp Met Tyr - # Met Pro Arg Asp Ile Thr AlaGly# 540- Asp Ile Val Thr Leu Thr Ser Cys - # Gln Ala Asn Tyr Val Ser ValXaa# 560- Lys Thr Val Ile Thr Thr Xaa Val - # Asp Asn Asp Asp Phe Asp PheAsp# 575- Asp Glu Leu Ser Lys Trp Trp Asn - # Asp Thr Lys His Glu Leu ProAsp# 590- Phe Asp Glu Phe Asn Tyr Thr Val - # Pro Ile Leu Asp Ile Gly SerGlu# 605- Ile Asp Arg Ile Gln Gly Val Ile - # Gln Gly Leu Asn Asp Ser LeuIle# 620- Asp Leu Glu Thr Leu Ser Ile Leu - # Lys Thr Tyr Ile Lys Trp ProTrp# 640- Tyr Val Trp Leu Ala Ile Ala Phe - # Xaa Thr Ile Ile Phe Ile LeuIle# 655- Leu Gly Trp Val Phe Phe Met Thr - # Gly Cys Cys Gly Cys Cys CysGly# 670- Cys Phe Gly Ile Ile Pro Leu Met - # Ser Lys Cys Gly Lys Lys SerSer# 685- Tyr Tyr Thr Thr Leu Asp Asn Asp - # Val Val Thr Glu Gln Xaa ArgPro# 700- Lys 705- (2) INFORMATION FOR SEQ ID NO:20:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 36 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:# 36 GGGG ATCCTCTAGA GTCGAC- (2) INFORMATION FOR SEQ ID NO:21:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 57 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 13..57- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:- CACAGCTCAA CA ATG AAG TGG GCA ACG TGG ATC GAT - # CCC GTC GTT TTA 48 Met Lys Tr - #p Ala Thr Trp Ile Asp Pro Val Val Leu# 10# 57Gln Arg Arg 15- (2) INFORMATION FOR SEQ ID NO:22:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 15 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:- Met Lys Trp Ala Thr Trp Ile Asp Pro Val Va - #l Leu Gln Arg Arg# 15- (2) INFORMATION FOR SEQ ID NO:23:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 57 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:- ACTCGGGCAG CGTTGGGTCC TGGGACTCTA GAGGATCGAT CCCCTATGGC GA - #TCATC 57- (2) INFORMATION FOR SEQ ID NO:24:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 99 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24:- GCGCCCACGT GGCCTGGTAC AATTCGAGCT CGCCCGGGGA TCCTCTAGAG TC - #GACTCTAG 60# 99 AGTC GGCGGGACGA GCCCGCGAT- (2) INFORMATION FOR SEQ ID NO:25:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 57 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25:- TCCACAGGAC CTGCAGCGAC CCGCTTAACA GCGTCAACAG CGTGCCGCAG AT - #CGGGG 57- (2) INFORMATION FOR SEQ ID NO:26:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 35 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:# 35 GGGG GAGGCTAACT GAAAC- (2) INFORMATION FOR SEQ ID NO:27:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 103 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27:- GCTCATGGTG GCCCCCGGGC GGTTCAACGA GGGCCAGTAC CGGCGCCTGG TG - #TCCGTCGA 60#103 AGAG GATCCCCGGG CGAGCTCGAA TTC- (2) INFORMATION FOR SEQ ID NO:28:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 66 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28:- GAATTCGAGC TCGCCCGGGG ATCCTCTAGA GTCGACGTCT GGGGCGCGGG GG - #TGGTGCTC 60# 66- (2) INFORMATION FOR SEQ ID NO:29:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 66 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 16..66- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29:#GAT CCC GTC GTT TTA 51GG GCA ACG TGG ATC#Lys Trp Ala Thr Trp Ile Asp Pro Val Val L - #eu# 10# 66 GGGln Arg Arg Asp Trp 15- (2) INFORMATION FOR SEQ ID NO:30:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 17 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:- Met Lys Trp Ala Thr Trp Ile Asp Pro Val Va - #l Leu Gln Arg Arg Asp# 15- Trp- (2) INFORMATION FOR SEQ ID NO:31:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 132 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..93- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:- GAC GAC TCC TGG AGC CCG TCA GTA TCG GCG GA - #A ATC CAG CTG AGC GCC 48Asp Asp Ser Trp Ser Pro Ser Val Ser Ala Gl - #u Ile Gln Leu Ser Ala# 15- GGT CGC TAC CAT TAC CAG TTG GTC TGG TGT CA - #A AAA GAT CTA GAA#93Gly Arg Tyr His Tyr Gln Leu Val Trp Cys Gl - #n Lys Asp Leu Glu# 30# 132 TCCC CTATGGCGAT CATCAGGGC- (2) INFORMATION FOR SEQ ID NO:32:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 31 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32:- Asp Asp Ser Trp Ser Pro Ser Val Ser Ala Gl - #u Ile Gln Leu Ser Ala# 15- Gly Arg Tyr His Tyr Gln Leu Val Trp Cys Gl - #n Lys Asp Leu Glu# 30- (2) INFORMATION FOR SEQ ID NO:33:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 66 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:- AACGAGGGCC AGTACCGGCG CCTGGTGTCC GTCGACTCTA GAGGATCCCC GG - #GCGAGCTC 60# 66- (2) INFORMATION FOR SEQ ID NO:34:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 65 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34:- CAGGTCGAAG CTTGGGCGCT GCCTATGTAG TGAAATCTAT ACTGGGATTT AT - #CATAACTA 60# 65- (2) INFORMATION FOR SEQ ID NO:35:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 65 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35:- AATAATCTAT CACTTTGTCA TGGAGATGCC CAAGCTTCGA CGACTCCCTT GG - #CCATGATG 60# 65- (2) INFORMATION FOR SEQ ID NO:36:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 65 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36:- TATACCAGCT ACGGCGCTAG CATTCATGGT ATCCCGTGAT TGCTCGATGC TT - #TCCTTCTG 60# 65- (2) INFORMATION FOR SEQ ID NO:37:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 65 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37:- AAGCTTGGCC TCGTCGTTAA TTAACCCAAT TCGAGCTCGC CCAGCTTGGG CT - #GCAGGTCG 60# 65- (2) INFORMATION FOR SEQ ID NO:38:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 65 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38:- TGTTTCAGTT AGCCTCCCCC ATCTCCCGAC TCTAGAGGAT CTCGACATAG CG - #AATACATT 60# 65- (2) INFORMATION FOR SEQ ID NO:39:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 130 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39:- AACGTATATA TTTTTCACGA CGTAGACCAC TATTGCCATG GACTCTAGAG GA - #TCGGGTAC 60- CGAGCTCGAA TTGGGAAGCT TGTCGACTTA ATTAAGCGGC CGCGTTTAAA CG - #GCCCTCGA 120# 130- (2) INFORMATION FOR SEQ ID NO:40:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 60 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40:- GTCGACGTCT GGGGCGCGGG GGTGGTGCTC TTCGAGACGC TGCCTACCCC AA - #GACGATCG 60- (2) INFORMATION FOR SEQ ID NO:41:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 60 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41:- AGCTCAACAA TGAAGTGGGC AACGTGGATC GATCCCGTCG TTTTACAACG TC - #GTGACTGG 60- (2) INFORMATION FOR SEQ ID NO:42:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 120 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42:- GAGCCCGTCA GTATCGGCGG AAATCCAGCT GAGCGCCGGT CGCTACCATT AC - #CAGTTGGT 60- GTTGGTCTGG TGTCAAAAAG ATCCGGACCG CGCCGTTAGC CAAGTTGCGT TA - #GAGAATGA 120- (2) INFORMATION FOR SEQ ID NO:43:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 60 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43:- ACACAGTCAC ACTCATGGGG GCCGAAGGCA GAATTCGTAA TCATGGTCAT AG - #CTGTTTCC 60- (2) INFORMATION FOR SEQ ID NO:44:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 60 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44:- AAACCTGTCG TGCCAGCGAG CTCGGGATCC TCTAGAGGAT CCCCGGGCCC CG - #CCCCCTGC 60- (2) INFORMATION FOR SEQ ID NO:45:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 60 base# (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45:- TCGTCCACAC GGAGCGCGGC TGCCGACACG GATCCCGGTT GGCGCCCTCC AG - #GTGCAGGA 60- (2) INFORMATION FOR SEQ ID NO:46:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 60 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46:- AACCCCCCCC CCCCCCCCCC CCCCCCCCTG CAGGCATCGT GGTGTCACGC TC - #GTCGTTTG 60- (2) INFORMATION FOR SEQ ID NO:47:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 60 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (iii) HYPOTHETICAL: NO- (iv) ANTI-SENSE: NO- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47:- TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TCGGATCCTC TA - #GAGTCGAC 60__________________________________________________________________________

Number	Name	Date
4877737	Shih et al.	Oct 1989
5047237	Cochran	Sep 1991
5171677	Sakaguchi et al.	Dec 1992
5187087	Sondermeijer et al.	Feb 1993
5223424	Cochran et al.	Jun 1993
5225336	Paoletti	Jul 1993
5240703	Cochran	Aug 1993
5252717	Velicer et al.	Oct 1993
5266489	Rey-Senelonge et al.	Nov 1993
5686287	Baxendale	Nov 1997

Number	Date	Country
0431668	Jun 1991	EPX
447303	Sep 1991	EPX
473210	Mar 1992	EPX
477056	Mar 1992	EPX
486106	May 1992	EPX
WO 8807088	Sep 1988	WOX
WO 8901040	Feb 1989	WOX
WO 9002802	Mar 1990	WOX
9002803	Mar 1990	WOX
WO 9203554	Mar 1992	WOX
9215672	Sep 1992	WOX

	Number	Date	Country
	225032	Jul 1988
	902887	Sep 1986
	823102	Jan 1986
	773430	Sep 1985
	902877	Sep 1986
	887140	Jul 1986
	823102	Jan 1986
	773403	Sep 1985
	823102	Jan 1986

	Number	Date	Country
Parent	078519	Jul 1987
Parent	933107	Nov 1986

	Number	Date
Parent	898087	Jun 1992
Parent	078519	Jul 1987
Parent	649380	Jan 1991
Parent	933107	Nov 1986
Parent	914057	Jul 1992
Parent	696262	Apr 1991
Parent	773430	Sep 1985

Recombinant herpesvirus of turkeys and uses thereof

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