RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES

Abstract
The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Description
FIELD OF THE INVENTION

The invention relates generally to the field of industrial microbiology. The invention relates to recombinant host cells comprising (i) a modification in an endogenous gene encoding a polypeptide that converts pyruvate to acetyl-CoA, acetaldehyde or acetyl-phosphate and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The invention also relates to recombinant host cells comprising (i) a modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase (PDC) activity, or a modification in an endogenous polypeptide having PDC activity, and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The invention also relates to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing cell growth, methods of reducing or eliminating the requirement of an exogenous carbon substrate for cell growth, methods of increasing glucose consumption and methods of increasing the production of a product of a pyruvate-utilizing pathway.


BACKGROUND OF THE INVENTION

Global demand for liquid transportation fuel is projected to strain the ability to meet certain environmentally driven goals, for example, the conservation of oil reserves and limitation of green house gas emissions. Such demand has driven the development of technology which allows utilization of renewable resources to mitigate the depletion of oil reserves and to minimize green house gas emissions.


Butanol is an important industrial chemical, useful as a fuel additive, as a feedstock chemical in the plastics industry, and as a food grade extractant in the food and flavor industry. Each year 10 to 12 billion pounds of butanol are produced by petrochemical means and the need for this commodity chemical will likely increase in the future.


Methods for the chemical synthesis of isobutanol are known, such as oxo synthesis, catalytic hydrogenation of carbon monoxide (Ullmann's Encyclopedia of Industrial Chemistry, 6th edition, 2003, Wiley-VCH Verlag GmbH and Co., Weinheim, Germany, Vol. 5, pp. 716-719) and Guerbet condensation of methanol with n-propanol (Carlini et al., J. Molec. Catal. A: Chem. 220:215-220, 2004). These processes use starting materials derived from petrochemicals, are generally expensive, and are not environmentally friendly. The production of isobutanol from plant-derived raw materials would minimize green house gas emissions and would represent an advance in the art.


2-Butanone, also referred to as methyl ethyl ketone (MEK) is a widely used solvent and is the most important commercially produced ketone, after acetone. It is used as a solvent for paints, resins, and adhesives, as well as a selective extractant, activator of oxidative reactions, and it can be chemically converted to 2-butanol by reacting with hydrogen in the presence of a catalyst (Nystrom, R. F. and Brown, W. G. (J. Am. Chem. Soc. (1947) 69:1198). 2,3-butanediol can be used in the chemical synthesis of butene and butadiene, important industrial chemicals currently obtained from cracked petroleum, and esters of 2,3-butanediol may be used as plasticizers (Voloch et al., “Fermentation Derived 2,3-butanediol,” in Comprehensive Biotechnology, Pergamon Press Ltd., England Vol. 2, Section 3:933-947 (1986)).


Microorganisms can be engineered for the expression of biosynthetic pathways that initiate with cellular pyruvate to produce, for example, 2,3-butanediol, 2-butanone, 2-butanol and isobutanol. U.S. Pat. No. 7,851,188 discloses the engineering of recombinant microorganisms for production of isobutanol. U.S. Patent Application Publication Nos. US 20070259410 A1 and US 20070292927 A1 disclose the engineering of recombinant microorganisms for production of 2-butanone or 2-butanol. Multiple pathways are disclosed for biosynthesis of isobutanol and 2-butanol, all of which initiate with cellular pyruvate. Butanediol is an intermediate in the 2-butanol pathway disclosed in U.S. Patent Application Publication No. US 20070292927 A1.


The disruption of the enzyme pyruvate decarboxylase (PDC) in recombinant host cells engineered to express a pyruvate-utilizing biosynthetic pathway has been used to increase the availability of pyruvate for product formation via the biosynthetic pathway. For example, U.S. Application Publication No. US 20070031950 A1 discloses a yeast strain with a disruption of one or more pyruvate decarboxylase genes (a PDC knock-out or PDC-KO) and expression of a D-lactate dehydrogenase gene, which is used for production of D-lactic acid. U.S. Application Publication No. US 20050059136 A1 discloses glucose tolerant two-carbon source-independent (GCSI) yeast strains with no PDC activity, which may have an exogenous lactate dehydrogenase gene. Nevoigt and Stahl (Yeast 12:1331-1337 (1996)) describe the impact of reduced PDC and increased NAD-dependent glycerol-3-phosphate dehydrogenase iii Saccharomyces cerevisiae on glycerol yield. U.S. Application Publication No. 20090305363 A1 discloses increased conversion of pyruvate to acetolactate by engineering yeast for expression of a cytosol-localized acetolactate synthase and substantial elimination of PDC activity.


While PDC-KO recombinant host cells can be used to produce the products of pyruvate-utilizing biosynthetic pathways, PDC-KO recombinant host cells require exogenous carbon substrate supplementation (e.g., ethanol or acetate) for their growth (Flikweert et al. 1999. FEMS Microbiol. Lett. 174(1):73-79 “Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae”). A similar auxotrophy is observed in Escherichia coli strains carrying a mutation of one or more genes encoding pyruvate dehydrogenase (Langley and Guest, 1977, J. Gen, Microbiol. 99:263-276).


In commercial applications, addition of exogenous carbon substrate in addition to the substrate converted to a desired product can lead, to increased costs. There remains a need in the art for recombinant host cells with reduced or eliminated need for exogenous carbon substrate supplementation.


BRIEF SUMMARY OF THE INVENTION

One aspect of the invention relates to a recombinant host cell comprising (i) least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. Another aspect of the invention relates to such a recombinant host cell further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. In embodiments, the polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA is pyruvate decarboxylase, pyruvate-formate lyase, pyruvate dehydrogenase, pyruvate oxidase, or pyruvate:ferredoxin oxidoreductase.


One aspect of the invention relates to a recombinant host cell comprising (i) a modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity or in an endogenous polypeptide having pyruvate decarboxylase activity; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. Another aspect of the invention relates to such a recombinant host cell further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


One aspect of the invention relates to a recombinant host cell comprising (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. Another aspect of the invention relates to a recombinant host cell further comprising: (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. Another aspect of invention relates to a reduced or eliminated requirement of such cells for an exogenous two-carbon substrate for its growth in culture compared to a recombinant eukaryotic host cell comprising (i) and not (ii) or (iii). Another aspect of the invention relates to the growth of such host cells in culture media that is not supplemented with an exogenous two-carbon substrate, for example, at a growth rate substantially equivalent to, or greater than, the growth rate of a host cell comprising (i) and not (ii) or (iii) in culture media supplemented with an exogenous two-carbon substrate.


In one aspect of the invention, the recombinant host cell is a member of the genera Clostridium, Zymomonas, Escherichia, Salmonella, Serratia, Erwinia, Klebsiella, Shigella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Schizosaccharomyces, Kluyveromyces, Yarrowia, Pichia, Candida, Hansenula, or Saccharomyces. In another aspect of the invention, the recombinant host cell is S. cerevisiae


In another aspect of the invention, the recombinant host cell expresses a pyruvate-utilizing biosynthetic pathway including, for example, a biosynthetic pathway for a product such as 2,3-butanediol, isobutanol, 2-butanol, 2-butanone, valine, leucine, alanine, lactic acid, malic acid, fumaric acid, succinic acid, or isoamyl alcohol. Another aspect of the invention relates to expression of an isobutanol biosynthetic pathway in the recombinant host cell comprising at least one DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion selected from the group consisting of (i) pyruvate to acetolactate; (ii) acetolactate to 2,3-dihydroxyisovalerate; (iii) 2,3-dihydroxyisovalerate to 2-ketoisovalerate; (iv) 2-ketoisovalerate to isobutyraldehyde; and (v) isobutyraldehyde to isobutanol. Another aspect of the invention relates to expression of a 2-butanone biosynthetic pathway in the recombinant host cell comprising at least one DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion selected from the group consisting of (i) pyruvate to acetolactate; (ii) acetolactate to acetoin; (iii) acetoin to 2,3-butanediol; and (iv) 2,3-butanediol to 2-butanone.


Another aspect of the invention relates to expression of a 2-butanol biosynthetic pathway in the recombinant host cell comprising at least one DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion selected from the group consisting of (i) pyruvate to acetolactate; (ii) acetolactate to acetoin; (iii) acetoin to 2,3-butanediol; (iv) 2,3-butanediol to 2-butanone; and (v) 2-butanone to 2-butanol.


One aspect of the invention relates to methods for the production of a product selected from the group consisting of 2,3-butanediol, isobutanol, 2-butanol, 2-butanone, valine, leucine, alanine, lactic acid, malic acid, fumaric acid, succinic acid and isoamyl alcohol comprising growing the recombinant host cells described herein under conditions wherein the product is produced and optionally recovering the product. Another aspect of the invention relates to methods of producing a recombinant host cell comprising transforming a host cell comprising at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity with (i) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity; and optionally (ii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


Another aspect of the invention relates to methods of improving the growth of a recombinant host cell comprising at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, comprising (i) transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity; and optionally (ii) transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. In embodiments, the methods further comprise growing the recombinant host cell in media containing limited carbon substrate.


Another aspect of the invention relates to methods of reducing the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, comprising (i) transforming the host cell with a heterologous polynucleotide encoding, a polypeptide having, phosphoketolase activity; and optionally (ii) transforming the host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


Another aspect of the invention relates to methods of eliminating the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, comprising (i) transforming the host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity; and optionally (ii) transforming the host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


Still another aspect of the invention relates to methods for increasing the activity of the phosphoketolase pathway in a recombinant host cell comprising (i) providing a recombinant host cell of the invention; and (ii) growing the recombinant host cell under conditions whereby the activity of the phosphoketolase pathway in the recombinant host cell is increased.


In another aspect, the recombinant host cells comprise a phosphoketolase that matches the Profile HMM given in Table 6 with an E value of less than 7.5E-242. In another aspect, the phosphoketolase has at least about 40% identity to at least one of SEQ ID NO 355, 379, 381, 388, 481, 486, 468, or 504. In another aspect, the phosphoketolase has at least about 90% identity to at least one of SEQ ID NO: 355, 379, 381, 388, 481, 486, 468, or 504. In another aspect, the phosphoketolase matches the Profile HMMs given in Tables 6, 7, 8, and 9 with E values of less than 7.5E-242, 1.1E-124, 2.1E-49, 7.8E-37, respectively. In another aspect, the recombinant host cells further comprise a phosphotransacetylase which matches the Profile HMM given in Table 14 with an E value of less than 5E-34. In another aspect, the phosphotransacetylase has at least about 40% identity to SEQ ID NO: 1475, 1472, 1453, 1422, 1277, 1275, 1206, 1200, 1159, or 1129. In another aspect, the phosphotransacetylase has at least about 90% identity to SEQ ID NO: 1475, 1472, 1453, 1422, 1277, 1275, 1206, 1200, 1159, or 1129





BRIEF DESCRIPTION OF THE DRAWINGS/FIGURES AND INCORPORATION OF SEQUENCE LISTING AND TABLES

The various embodiments of the invention can be more fully understood from the detailed description, the figures, and the accompanying sequence descriptions, which form a part of this application.



FIG. 1 depicts a schematic representation of the phosphoketolase pathway, including the phosphoketolase and phosphotransacetylase enzymes.



FIG. 2 depicts the growth of PDC-KO yeast strains expressing phosphoketolase and phosphotransacetylase without exogenous carbon substrate supplementation.



FIG. 3 depicts the growth of PDC-KO yeast strains expressing phosphoketolase and/or phosphotransacetylase in without exogenous carbon substrate supplementation.



FIG. 4 depicts a phylogenetic tree of phosphate acetyltransferase (PTA) and phosphate butyryltransferase (PTB) sequences. Multiple sequence alignment was performed with Clustal X using default parameters. Phylogenetic tree was deduced using neighbor-joining method and drawn with Mega 4 software. Marked sequences are as follows: (#, Species, GI#) 1, S. enterica, 56412650; 2, E. coli K12, 88192043; 3, V. parvula, 227371784; 4, C. kluyveri, 153954015; 5, C. Acetobutylicum, 15895019; 6, C. thermocellum, 196254011; 7, M. thermophila, 88192043; 8, S. pyogenes, 48425286; 9, B. subtilis, 58176784; 10, L. fermentum, 227514417; 11, L. plantarum, 28377658; 12, L. sanfranciscensis, 11862872;



FIG. 5 is a plasmid map of pRS426::GPD-xpk1+ADH-eutD map which is described herein.



FIG. 6 depicts the Δpdc1::ilvD(Sm) locus of BP913 after integration of a phosphoketolase pathway vector (described herein).



FIG. 7A shows the growth of an isobutanol-producing strain in the absence (no ETOH) and presence (+ETOH) of EtOH and the absence and presence of the phosphoketolase pathway (xpk). ISO1, ISO2 and ISO3 refer to replicates.



FIG. 7B shows the growth of a second subculture of strains from FIG. 7A.





Tables 6, 7, 8, 9, and 14 are tables of the Profile HMMs described herein. Table 6, 7, 8, and 14 are submitted herewith electronically and are incorporated herein by reference.


The invention can be more fully understood from the following detailed description and the accompanying sequence descriptions which form a part of this application.


The sequence listing provided herewith is herein incorporated by reference and conforms with 37 C.F.R. 1.821-1.825 (“Requirements for Patent Applications Containing Nucleotide Sequences and/or Amino Acid Sequence Disclosures—the Sequence Rules”) and is consistent with World Intellectual Property Organization (WIPO) Standard ST.25 (2009) and the sequence listing requirements of the EPO and PCT (Rules 5.2 and 49.5 (a-bis), and Section 208 and Annex C of the Administrative Instructions). The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822. The content of the electronically submitted sequence listing Name: 20110615_CL4871USNA_SeqList.txt; Size: 6.67 MB; and Date of Creation/Modification: Jun. 9, 2011/Jun. 15, 2011 is incorporated herein by reference in its entirety.


SEQ ID NOs: 1-20 are sequences of PDC target gene coding regions and proteins.


SEQ ID NOs: 21-638 are phosphoketolase target gene coding regions and proteins.


SEQ ID NOs: 762-1885 are phosphotransacetylase target gene coding regions and proteins.


SEQ ID NOs: 1893-1897 are hybrid promoter sequences.


SEQ ID NOs: 639-642, 644-654, 656-660, 662-701-714, 725-726, 729-740, 742-748, and 750-761 are primers.


SEQ ID NO: 643 is the vector pRS426::GPD-xpk1+ADH1-eutD.


SEQ ID NO: 655 is the TEF1p-kan-TEF1t gene.


SEQ ID NO: 661 is vector pLA54.


SEQ ID NO: 715 is vector pRS423::pGAL1-cre.


SEQ ID NO: 716 is the vector pLH468-sadB.


SEQ ID NOs: 717 and 718 are the amino acid and nucleic acid sequences for sadB from Achromobacter xylosoxidans.


SEQ ID NO: 719 is the kivD coding region from L. lactis.


SEQ ID NO: 720 is the plasmid pRS425::GPM-sadB.


SEQ ID NO: 721 is the GPM promoter.


SEQ ID NO: 722 is the ADH1 terminator.


SEQ ID NO: 723 is the GPM-sadB-ADHt segment.


SEQ ID NO: 724 is the pUC19-URA3 plasmid.


SEQ ID NO: 741 is the ilvD-FBA1t segment.


SEQ ID NO 749 is URA3r2 template DNA.


SEQ ID NO: 1886 is the ilvD coding region from S. mutans.


SEQ ID NO: 1888 is vector 011468.


SEQ ID NO: 1898 is pUC19-URA3::pdc1::GPD-xpk1+ADH1-eutD.


SEQ ID NOs: 1899-1906 are the sequences of modified S. cerevisiae loci.


SEQ ID NO: 1907 is the sequence of pLH702.


SEQ ID NO: 1908 is the sequence of pYZ067DkivDDhADH


SEQ ID NO: 1909 is the amino acid sequence of ALD6.


SEQ ID NO: 1910 is the amino acid sequence of K9D3.


SEQ ID NO: 1911 is the amino acid sequence of K9G9.


SEQ ID NO: 1912 is the amino acid sequence of YMR226c.


SEQ ID NOs: 1913 and 1914 are the nucleic acid and amino acid sequences of AFT1.


SEQ ID NOs: 1915 and 1916 are the nucleic acid and amino acid sequences of AFT2.


SEQ ID NOs: 1917 and 1918 are the nucleic acid and amino acid sequences of FRA2.


SEQ ID NOs. 1919 and 1920 are the nucleic acid and amino acid sequences of GRx3.


SEQ ID NOs: 1921 and 1922 are the nucleic acid and amino acid sequences of CCC1.


SEQ ID NO: 1923 is the amino acid sequence of an alcohol dehydrogenase from Beijerinkia indica.


DETAILED DESCRIPTION OF THE INVENTION

Applicants have solved the stated problem by reducing or eliminating the need for providing two substrates, one of which is converted to a desired product, the other fully or partly into acetyl-CoA by recombinant host cells requiring such supplementation for growth comprising the expression of enzymes of the phosphoketolase pathway in such cells. One such enzyme, phosphoketolase (Enzyme Commission Number EC 4.1.2.9), catalyzes the conversion of xylulose 5-phosphate into glyceraldehyde 3-phosphate and acetyl-phosphate (Heath et al., J. Biol. Chem. 231: 1009-29; 1958). Another such enzyme is phosphotransacetylase (Enzyme Commission Number EC 2.3.1.8) which converts acetyl-phosphate into acetyl-CoA.


Applicants have provided PDC-KO recombinant host cells comprising a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity, and optionally a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. Such cells exhibit a reduced or eliminated requirement for exogenous two-carbon substrate supplementation for their growth compared to PDC-KO cells. Applicants have also provided methods of making and using such recombinant host cells including, for example, methods of increasing cell growth, methods of reducing or eliminating the requirement of an exogenous two-carbon substrate for cell growth, methods of increasing glucose consumption and methods of increasing the production of a product of a pyruvate-utilizing pathway.


Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present application including the definitions will control. Unless otherwise required, by context, singular terms shall include pluralities and plural terms shall include the, singular. All publications, patents and other references mentioned herein are incorporated by reference in their entireties for all purposes as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference, unless only specific sections of patents or patent publications are indicated to be incorporated by reference.


Although methods and materials similar or equivalent to those described herein can be used in practice or testing of the present invention, suitable methods and materials are described below. The materials, methods and examples are illustrative only and are not intended to be limiting. Other features and advantages of the invention will be apparent from the detailed description and from the claims.


In order to further define this invention, the following terms, abbreviations and definitions are provided.


As used herein, the terms “comprises,” “comprising,” “includes,” “including,” “has,” “having,” “contains,” or “containing,” or any other variation thereof, are intended to be non-exclusive or open-ended. For example, a composition, a mixture, a process, a method, an article, or an apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, mixture, process, method, article, or apparatus. Further, unless expressly stated to the contrary, “or” refers to an inclusive or and not to an exclusive or. For example, a condition A or B is satisfied by any one of the following: A is true (or present) and B is false (or not present), A is false (or not present) and B is true (or present), and both A and B are true (or present).


Also, the indefinite articles “a” and “an” preceding an element or component of the invention are intended to be nonrestrictive regarding the number of instances, i.e., occurrences of the element or component. Therefore “a” or “an” should be read to include one or at least one, and the singular word form of the element or component also includes the plural unless the number is obviously meant to be singular.


The term “invention” or “present invention” as used herein is a non-limiting term and is not intended to refer to any single embodiment of the particular invention but encompasses all possible embodiments as described in the application.


As used herein, the term “about” modifying the quantity of an ingredient or reactant of the invention employed refers to variation in the numerical quantity that can occur, for example, through typical measuring and liquid handling procedures used for making concentrates or use, solutions in the real world; through inadvertent error in these procedures; through differences in the manufacture, source, or purity of the ingredients employed to make the compositions or to carry out the methods; and the like. The term “about” also encompasses amounts that differ due to different equilibrium conditions for a composition resulting from a particular initial mixture. Whether or not modified by the term “about”, the claims include equivalents to the quantities. In one embodiment, the term “about” means within 10% of the reported numerical value, preferably within 5% of the reported numerical value.


The term “butanol” as used herein, refers to 2-butanol, 1-butanol, isobutanol, or mixtures thereof.


The term “pyruvate-utilizing biosynthetic pathway” refers to an enzyme pathway to produce a biosynthetic product from pyruvate.


The term “isobutanol biosynthetic pathway” refers to an enzyme pathway to produce isobutanol from pyruvate.


The term “2-butanol biosynthetic pathway” refers to an enzyme pathway to produce 2-butanol from pyruvate.


The term “2-butanone biosynthetic pathway” refers to an enzyme pathway to produce 2-butanone from pyruvate.


The terms “pdc-,” “PDC knock-out,” or “PDC-KO” as used herein refer to a cell that has a genetic modification to inactivate or reduce expression of at least one gene encoding pyruvate decarboxylase (PDC) so that the cell substantially or completely lacks pyruvate decarboxylase enzyme activity. If the cell has more than one expressed (active) PDC gene, then each of the active PDC genes may be inactivated or have minimal expression thereby producing a pdc-cell.


The term “carbon substrate” refers to a carbon source capable of being metabolized by the recombinant host cells disclosed herein. Non-limiting examples of carbon substrates are provided herein and include, but are not limited to, monosaccharides, oligosaccharides, polysaccharides, ethanol, lactate, succinate, glycerol, carbon dioxide, methanol, glucose, fructose, sucrose, xylose, arabinose, dextrose, or mixtures thereof.


The term “exogenous two-carbon substrate” refers to the carbon source provided to be metabolized into acetyl-CoA by a host cell that lacks the ability to convert pyruvic acid into acetyl-CoA. The term is used to distinguish from the carbon substrate which is converted into a pyruvate-derived product by a pyruvate-utilizing biosynthetic pathway, herein also referred to as the “pathway substrate” which includes, for example, glucose.


The term “polynucleotide” is intended to encompass a singular nucleic acid as well as plural nucleic acids, and refers to a nucleic acid molecule or construct, e.g., messenger RNA (mRNA) or plasmid DNA (pDNA). A polynucleotide can contain the nucleotide sequence of the full-length cDNA sequence, or a fragment thereof, including the untranslated 5′ and 3′ sequences and the coding sequences. The polynucleotide can be composed of any polyribonucleotide or polydeoxyribonucleotide, which may be unmodified RNA or DNA or modified RNA or DNA. For example, polynucleotides can be composed of single- and double-stranded DNA, DNA that is a mixture of single- and double-stranded regions, single- and double-stranded RNA, and RNA that is mixture of single- and double-stranded regions, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded or a mixture of single- and double-stranded regions. “Polynucleotide” embraces chemically, enzymatically, or metabolically modified forms.


A polynucleotide sequence may be referred to as “isolated,” in which it has been removed from its native environment. For example, a heterologous polynucleotide encoding a polypeptide or polypeptide fragment having dihydroxy-acid dehydratase activity contained in a vector is considered isolated for the purposes of the present invention. Further examples of an isolated polynucleotide include recombinant polynucleotides maintained in heterologous host cells or purified (partially or substantially) polynucleotides in solution. Isolated polynucleotides or nucleic acids according to the present invention further include such molecules produced synthetically. An isolated polynucleotide fragment in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA or synthetic DNA.


The term “gene” refers to a nucleic acid fragment that is capable of being expressed as a specific protein, optionally including regulatory sequences preceding (5′ non-coding sequences) and following (3′ non-coding sequences) the coding sequence. “Native gene” refers to a gene as found in nature with its own regulatory sequences. “Endogenous gene” refers to a native gene in its natural location in the genome of an organism. A “heterologous gene” refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. “Heterologous gene” includes a native coding region, or portion thereof, that is reintroduced into the source organism in a form that is different from the corresponding native gene. For example, a heterologous gene may include a native coding region that is a portion of a chimeric gene including non-native regulatory regions that is reintroduced into the native host. Foreign genes can comprise native genes inserted into a non-native organism, or chimeric genes.


As used herein the term “coding region” refers to a DNA sequence that codes for a specific amino acid sequence. “Suitable regulatory sequences” refer to nucleotide sequences located upstream (5′ non-coding sequences), within, or downstream (3′ non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include promoters, translation leader sequences, introns, polyadenylation recognition sequences, RNA processing site, effector binding site and stem-loop structure.


As used herein, the term “polypeptide” is intended to encompass a singular “polypeptide” as well as plural “polypeptides,” and refers to a molecule composed of monomers (amino acids) linearly linked by amide bonds (also known as peptide bonds). The term “polypeptide” refers to any chain or chains of two or more amino acids, and does not refer to a specific length of the product. Thus, peptides, dipeptides, tripeptides, oligopeptides, “protein,” “amino acid chain,” or any other term used to refer to a chain or chains of two or more amino acids, are included within the definition of “polypeptide,” and the term “polypeptide” may be used instead of, or interchangeably with any of these terms. A polypeptide may be derived from a natural biological source or produced by recombinant technology, but is not necessarily translated from a designated nucleic acid sequence. It may be generated in any manner, including by chemical synthesis.


By an “isolated” polypeptide or a fragment, variant, or derivative thereof is intended a polypeptide that is not in its natural milieu. No particular level of purification is required. For example, an isolated polypeptide can be removed from its native or natural environment. Recombinantly produced polypeptides and proteins expressed in host cells are considered isolated for purposed of the invention, as are native or recombinant polypeptides which have been separated, fractionated, or partially or substantially purified by any suitable technique.


As used herein, the term “valiant” refers to a polypeptide differing from a specifically recited polypeptide of the invention by amino acid insertions, deletions, mutations, and substitutions, created using, e.g., recombinant DNA techniques, such as mutagenesis. Guidance in determining which amino acid residues may be replaced, added, or deleted without abolishing activities of interest, may be found by comparing the sequence of the particular polypeptide with that of homologous polypeptides, e.g., yeast or bacterial, and minimizing the number of amino acid sequence changes made in regions of high homology (conserved regions) or by replacing amino acids with consensus sequences.


Alternatively, recombinant polynucleotide variants encoding these same or similar polypeptides may be synthesized or selected by making use of the “redundancy” in the genetic code. Various codon substitutions, such as silent changes which produce various restriction sites, may be introduced to optimize cloning into a plasmid or viral vector for expression. Mutations in the polynucleotide sequence may be reflected in the polypeptide or domains of other peptides added to the polypeptide to modify the properties of any part of the polypeptide.


Amino acid “substitutions” may be the result of replacing one amino acid with another amino acid having similar structural and/or chemical properties, i.e., conservative amino acid replacements, or they may be the result of replacing, one amino acid with an amino acid having different structural and/or chemical properties, i.e., non-conservative amino acid replacements. “Conservative” amino acid substitutions may be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, or the amphipathic nature of the residues involved. For example, nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; positively charged (basic) amino acids include arginine, lysine, and, histidine; and negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Alternatively, “non-conservative” amino acid substitutions may be made by selecting the differences in polarity, charge, solubility, hydrophobicity, hydrophilicity, or the amphipathic nature of any of these amino acids. “Insertions” or “deletions” may be within the range of variation as structurally or functionally tolerated by the recombinant proteins. The variation allowed may be experimentally determined by systematically making insertions, deletions, or substitutions of amino acids in a polypeptide molecule using recombinant DNA techniques and assaying the resulting recombinant variants for activity.


The term “promoter” refers to a DNA sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3′ to a promoter sequence. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic DNA segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental or physiological conditions. Promoters which cause a gene to be expressed in most cell types at most times are commonly referred to as “constitutive promoters.” It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of different lengths may have identical promoter activity. For example, it will be understood that “FBA1 promoter” can be used to refer to a fragment derived from the promoter region of the FBA1 gene.


The term “terminator” as used herein refers to DNA sequences located downstream of a coding sequence. This includes polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3′ end of the mRNA precursor. The 3′ region can influence the transcription, RNA processing or stability, or translation of the associated coding sequence. It is recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of different lengths may have identical terminator activity. For example, it will be understood that “CYC1 terminator” can be used to refer to a fragment derived from the terminator region of the CYC1 gene.


The term “operably linked” refers to the association of nucleic acid sequences on a single nucleic acid fragment so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of effecting the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in sense or antisense orientation.


The term “expression,” as used herein, refers to the transcription and stable accumulation of sense (mRNA) or antisense RNA derived from the nucleic acid fragment of the invention. Expression may also refer to translation of mRNA into a polypeptide.


The term “overexpression,” as used herein, refers to expression that is higher than endogenous expression of the same or related gene. A heterologous gene is overexpressed if its expression is higher than that of a comparable endogenous gene.


As used herein the term “transformation” refers to the transfer of a nucleic acid fragment into a host organism, resulting in genetically stable inheritance. Host organisms containing the transformed nucleic acid fragments are referred to as “transgenic” or “recombinant” or “transformed” organisms.


The terms “plasmid” and “vector” as used herein, refer to an extra chromosomal element often carrying genes which are not part of the central metabolism of the cell, and usually in the form of circular double-stranded DNA molecules. Such elements may be autonomously replicating sequences, genome integrating sequences, phage or nucleotide sequences, linear or circular, of a single- or double-stranded DNA or RNA, derived from any source, in which a number of nucleotide sequences have been joined or recombined into a unique construction which is capable of introducing a promoter fragment and DNA sequence for a selected gene product along with appropriate 3′ untranslated sequence into a cell.


As used herein the term “codon degeneracy” refers to the nature in the genetic code permitting variation of the nucleotide sequence without affecting the amino acid sequence of an encoded polypeptide. The skilled artisan is well aware of the “codon-bias” exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a gene for improved expression in a host cell, it is desirable to design the gene such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell.


The term “codon-optimized” as it refers to genes or coding regions of nucleic acid molecules for transformation of various hosts, refers to the alteration of codons in the gene or coding regions of the nucleic acid molecules to reflect the typical codon usage of the host organism without altering the polypeptide encoded by the DNA. Such optimization includes replacing at least one, or more than one, or a significant number, of codons with one or more codons that are more frequently used in the genes of that organism.


Deviations in the nucleotide sequence that comprise the codons encoding the amino acids of any polypeptide chain allow for variations in the sequence coding for the gene. Since each codon consists of three nucleotides, and the nucleotides comprising DNA are restricted to four specific bases, there are 64 possible combinations of nucleotides, 61 of which encode amino acids (the remaining three codons encode signals ending translation). The “genetic code” which shows which codons encode which amino acids is reproduced herein as Table 1. As a result, many amino acids are designated by more than one codon. For example, the amino acids alanine and proline are coded for by four triplets, serine and arginine by six, whereas tryptophan and methionine are coded by just one triplet. This degeneracy allows for DNA base composition to vary over a wide range without altering the amino acid sequence of the proteins encoded by the DNA.









TABLE 1







The Standard Genetic Code












T
C
A
G





T
TTT Phe (F)
TCT Ser (S)
TAT Tyr (Y)
TGT Cys (C)



TTC ″
TTC ″
TAC ″
TGC



TTA Leu (L)
TCA ″
TAA Stop
TGA Stop



TTG ″
TCG ″
TAG Stop
TGG Trp (W)





C
CTT Leu (L)
CCT Pro (P)
CAT His (H)
CGT Arg (R)



CTC ″
CCC ″
CAC ″
CGC ″



CTA ″
CCA ″
CAA Gln (Q)
CGA ″



CTG ″
CCG ″
CAG ″
CGG ″





A
ATT Ile (I)
ACT Thr (T)
AAT Asn (N)
AGT Ser (S)



ATC ″
ACC ″
AAC ″
AGC ″



ATA ″
ACA ″
AAA Lys (K)
AGA Arg (R)



ATG Met (M)
ACG ″
AAG ″
AGG ″





G
GTT Val (V)
GCT Ala (A)
GAT Asp (D)
GGT Gly (G)



GTC ″
GCC ″
GAC ″
GGC ″



GTA ″
GCA ″
GAA Glu (E)
GGA ″



GTG ″
GCG ″
GAG ″
GGG ″









Many organisms display a bias for use of particular codons to code for insertion of a particular amino acid in a growing peptide chain. Codon preference, or codon bias, differences in codon usage between organisms, is afforded by degeneracy of the genetic code, and is well documented among many organisms. Codon bias often correlates with the efficiency of translation of messenger RNA (mRNA), which is in turn believed to be dependent on, inter alia, the properties of the codons being translated and the availability of particular transfer RNA (tRNA) molecules. The predominance of selected tRNAs in a cell is generally a reflection of the codons used most frequently in peptide synthesis. Accordingly, genes can be tailored for optimal gene expression in a given organism based on codon optimization.


Given the large number of gene sequences available for a wide variety of animal, plant and microbial species, it is possible to calculate the relative frequencies of codon usage. Codon usage tables are readily available, for example, at the “Codon Usage Database” available at http://www.kazusa.or.jp/codon/ (visited Mar. 20, 2008), and these tables can be adapted in a number of ways. See Nakamura, Y., et al. Nucl. Acids Res. 28:292 (2000). Codon usage tables for yeast, calculated from GenBank Release 128.0 [15 Feb. 2002], are reproduced below as Table 2. This table uses mRNA nomenclature, and so instead of thymine (T) which is found in DNA, the tables use uracil (U) which is found in RNA. Table 2 has been adapted so that frequencies are calculated for each amino acid, rather than for all 64 codons.









TABLE 2







Codon Usage Table for Saccharomyces



cerevisiae Genes
















Frequency per



Amino Acid
Codon
Number
thousand
















Phe
UUU
170666
26.1







Phe
UUC
120510
18.4







Leu
UUA
170884
26.2







Leu
UUG
177573
27.2







Leu
CUU
80076
12.3







Leu
CUC
35545
5.4







Leu
CUA
87619
13.4







Leu
CUG
68494
10.5







Ile
AUU
196893
30.1







Ile
AUC
112176
17.2







Ile
AUA
116254
17.8







Met
AUG
136805
20.9







Val
GUU
144243
22.1







Val
GUC
76947
11.8







Val
GUA
76927
11.8







Val
GUG
70337
10.8







Ser
UCU
153557
23.5







Ser
UCC
92923
14.2







Ser
UCA
122028
18.7







Ser
UCG
55951
8.6







Ser
AGU
92466
14.2







Ser
AGC
63726
9.8







Pro
CCU
88263
13.5







Pro
CCC
44309
6.8







Pro
CCA
119641
18.3







Pro
CCG
34597
5.3







Thr
ACU
132522
20.3







Thr
ACC
83207
12.7







Thr
ACA
116084
17.8







Thr
ACG
52045
8.0







Ala
GCU
138358
21.2







Ala
GCC
82357
12.6







Ala
GCA
105910
16.2







Ala
GCG
40358
6.2







Tyr
UAU
122728
18.8







Tyr
UAC
96596
14.8







His
CAU
89007
13.6







His
CAC
50785
7.8







Gln
CAA
178251
27.3







Gln
CAG
79121
12.1







Asn
AAU
233124
35.7







Asn
AAC
162199
24.8







Lys
AAA
273618
41.9







Lys
AAG
201361
30.8







Asp
GAU
245641
37.6







Asp
GAC
132048
20.2







Glu
GAA
297944
45.6







Glu
GAG
125717
19.2







Cys
UGU
52903
8.1







Cys
UGC
31095
4.8







Trp
UGG
67789
10.4







Arg
CGU
41791
6.4







Arg
CGC
16993
2.6







Arg
CGA
19562
3.0







Arg
CGG
11351
1.7







Arg
AGA
139081
21.3







Arg
AGG
60289
9.2







Gly
GGU
156109
23.9







Gly
GGC
63903
9.8







Gly
GGA
71216
10.9







Gly
GGG
39359
6.0







Stop
UAA
6913
1.1







Stop
UAG
3312
0.5







Stop
UGA
4447
0.7










By utilizing this or similar tables, one of ordinary skill in the art can apply the frequencies to any given polypeptide sequence, and produce a nucleic acid fragment of a codon-optimized coding region which encodes the polypeptide, but which uses codons optimal for a given species.


Randomly assigning codons at an optimized frequency to encode a given polypeptide sequence, can be done manually by calculating codon frequencies for each amino acid, and then assigning the codons to the polypeptide sequence randomly. Additionally, various algorithms and computer software programs are readily available to those of ordinary skill in the art. For example, the “EditSeq” function in the Lasergene Package, available from DNAstar, Inc., Madison, Wis., the backtransation function in the VectorNTI Suite, available from InforMax, Inc., Bethesda, Md., and the “backtranslate” function in the GCG-Wisconsin Package, available from Accelrys, Inc., San Diego, Calif. In addition, various resources are publicly available to codon-optimize coding region sequences, e.g., the “backtranslation” function at http://www.entelechon.com/bioinformatics/backtranslation.php?lang=eng (visited Apr. 15, 2008) and the “backtranseq” function available at http://bioinfo.pbi.nrc.ca:8090/EMBOSS/index.html (visited Jul. 9, 2002). Constructing a rudimentary algorithm to assign, codons based on a given frequency can also easily be accomplished with basic mathematical functions by one of ordinary skill in the art.


Codon-optimized coding regions can be designed by various methods known to those skilled in the art including software packages such as “synthetic gene designer” (http://phenotype.biosci.umbc.edu/codon/sal/index.php).


A polynucleotide or nucleic acid fragment is “hybridizable” to another nucleic acid fragment, such as a cDNA, genomic DNA, or RNA molecule, when a single-stranded form of the nucleic acid fragment can anneal to the other nucleic acid fragment under the appropriate conditions of temperature and solution ionic strength. Hybridization and washing conditions are well known and exemplified in Sambrook, J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboraton Manual, 2nd ed., Cold Spring Harbor Laboratory: Cold Spring Harbor, N.Y. (1989), particularly Chapter 11 and Table 11.1 therein (entirely incorporated herein by reference). The conditions of temperature and ionic strength determine the “stringency” of the hybridization. Stringency conditions can be adjusted to screen for moderately similar fragments (such as homologous sequences from distantly related organisms), to highly similar fragments (such as genes that duplicate functional enzymes from closely related organisms). Post-hybridization washes determine stringency conditions. One set of preferred conditions uses a series of washes starting with 6×SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. A more preferred set of stringent conditions uses higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2×SSC, 0.5% SDS was increased to 60° C. Another preferred set of highly stringent conditions uses two final washes in 0.1×SSC, 0.1% SDS at 65° C. An additional set of stringent conditions include hybridization at 0.1×SSC, 0.1% SDS, 65° C. and washes with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS, for example.


Hybridization requires that the two nucleic acids contain complementary sequences, although depending on the stringency of the hybridization, mismatches between bases are possible. The appropriate stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, variables well known in the art. The greater the degree of similarity or homology between two nucleotide sequences, the greater the value of Tm for hybrids of nucleic acids having those sequences. The relative stability (corresponding to higher Tin) of nucleic acid hybridizations decreases in the following order: RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100 nucleotides in length, equations for calculating Tm have been derived (see Sambrook et al., supra, 9.50-9.51). For hybridizations with shorter nucleic acids, i.e., oligonucleotides, the position of mismatches becomes more important, and the length of the oligonucleotide determines its specificity (see Sambrook et al., supra, 11.7-11.8). In one embodiment the length for a hybridizable nucleic acid is at least about 10 nucleotides. Preferably a minimum length for a hybridizable nucleic acid is at least about 15 nucleotides; more preferably at least about 20 nucleotides; and most preferably the length is at least about 30 nucleotides. Furthermore, the skilled artisan will recognize that the temperature and wash solution salt concentration may be adjusted as necessary according to factors such as length of the probe.


A “substantial portion” of an amino acid or nucleotide sequence is that portion comprising enough of the amino acid sequence, of a polypeptide or the nucleotide sequence of a gene to putatively identify that polypeptide or gene, either by manual evaluation of the sequence by one skilled in the art, or by computer-automated sequence comparison and identification using algorithms such as BLAST (Altschul, S. F., et al., J. Mol. Biol., 215:403-410 (199.3)). In general, a sequence often or more contiguous amino acids or thirty or more nucleotides is necessary in order to putatively identify a polypeptide or nucleic acid sequence as homologous to a known protein or gene. Moreover, with respect to nucleotide sequences, gene specific oligonucleotide probes comprising 20-30 contiguous nucleotides may be used in sequence-dependent methods of gene identification (e.g., Southern hybridization) and isolation (e.g., in situ hybridization of bacterial colonies or bacteriophage plaques). In addition, short oligonucleotides of 12-15 bases may be used as amplification primers in PCR in order to obtain a particular nucleic acid fragment comprising the primers. Accordingly, a “substantial portion” of a nucleotide sequence comprises enough of the sequence to specifically identify and/or isolate a nucleic acid fragment comprising the sequence. The instant specification teaches the complete amino acid and nucleotide sequence encoding particular proteins. The skilled artisan, having the benefit of the sequences as reported herein, may now use all or a substantial portion of the disclosed sequences for purposes known to those skilled in this art. Accordingly, the instant invention comprises the complete sequences as reported in the accompanying Sequence Listing, as well as substantial portions of those sequences as defined above.


The team “complementary” is used to describe the relationship between nucleotide bases that are capable of hybridizing to one another. For example, with respect to DNA, adenosine is complementary to thymine and cytosine is complementary to guanine.


The term “percent identity”, as known in the art, is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as determined by comparing the sequences. In the art, “identity” also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. “Identity” and “similarity” can be readily calculated by known methods, including but not limited to those described in 1.) Computational Molecular Biology (Lesk, A. M., Ed.) Oxford University: NY (1988); 2.) Biocomputing: Informatics and Genome Projects (Smith, D. W., Ed.) Academic: NY (1993); 3.) Computer Analysis of Sequence Data Part I (Griffin, A. M., and Griffin, H. G., Eds.) Humania: NJ (1994); 4.) Sequence Analysis in Molecular Biology (von Heinje, G., Ed.) Academic (1987); and 5.) Sequence Analysis Primer (Gribskov, M. and Devereux, J., Eds.) Stockton: NY (1991).


Preferred methods to determine identity are designed to give the best match between the sequences tested. Methods to determine identity and similarity are codified in publicly available computer programs. Sequence alignments and percent identity calculations may be performed using the MegAlign™ program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequences is performed using the “Clustal method of alignment” which encompasses several varieties of the algorithm including the “Clustal V method of alignment” corresponding to the alignment method labeled Clustal V (described by Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D. G. et al., Comput. Appl. Biosci., 8:189-191 (1992)) and found in the MegAlign™ program of the LASERGENE bioinformatics computing suite (DNASTAR Inc.). For multiple alignments, the default values correspond to GAP PENALTY=10 and GAP LENGTH PENALTY-10. Default parameters for pairwise alignments and calculation of percent identity of protein sequences using the Clustal method are KTUPLE=1, GAP PENALTY-3, WINDOW-5 and DIAGONALS SAVED=5. For nucleic acids these parameters are KTUPLE=2, GAP PENALTY=5, WINDOW=4 and DIAGONAS SAVED=4. After alignment of the sequences using the Clustal V program, it is possible to obtain a “percent identity” by viewing the “sequence distances” table in the same program. Additionally the “Clustal W method of alignment” is available and corresponds to the alignment method labeled Clustal W (described by Higgins and Sharp, CABIOS. 5:151-153 (1989); Higgins, D. G. et al., Comput. Appl. Biosci. 8:189-191 (1992)) and found in the MegAlign™ v6.1 program of the LASERGENE bioinformatics computing suite (DNASTAR Inc.). Default parameters for multiple alignment (GAP PENALTY=10, GAP LENGTH PENALTY=0.2, Delay Divergen Seqs(%)=30, DNA Transition Weight=0.5, Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB). After alignment of the sequences using the Clustal W program, it is possible to obtain a “percent identity” by viewing the “sequence distances” table in the same program.


It is well understood by one skilled in the art that many levels of sequence identity are useful in identifying polypeptides, such as from other species, wherein such polypeptides have the same or similar function or activity. Useful examples of percent identities include, but are not limited to: 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%, or any integer percentage from 55% to 100% may be useful in describing the present invention, such as 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%. Suitable nucleic acid fragments not only have the above homologies but typically encode a polypeptide having at least 50 amino acids, preferably at least 100 amino acids, more preferably at least 150 amino acids, still more preferably at least 200 amino acids, and most preferably at least 250 amino acids.


The term “sequence analysis software” refers to any computer algorithm or software program that is useful for the analysis of nucleotide or amino acid sequences. “Sequence analysis software” may be commercially available or independently developed. Typical sequence analysis software will include, but is not limited to: 1.) the GCG suite of programs (Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, Wis.); 2.) BLASTP, BLASTN, BLASTX (Altschul et al., J. Mol. Biol., 215:403-410 (1990)); 3.) DNASTAR (DNASTAR, Inc. Madison, Wis.); 4.) Sequencher (Gene Codes Corporation, Ann Arbor, Mich.); and 5.) the FASTA program incorporating the Smith-Waterman algorithm (W. R. Pearson, Comput. Methods Genome Res., [Proc. Int. Symp.] (1994), Meeting Date 1992, 111-20. Editor(s): Suhai, Samlor. Plenum: New York, N.Y.). Within the context of this application it will be understood that where sequence analysis software is used for analysis, that the results of the analysis will be based on the “default values” of the program referenced, unless otherwise specified. As used herein “default values” will mean any set of values or parameters that originally load with the software when first initialized.


Standard recombinant DNA and molecular cloning techniques used here are well known in the art and are described by Sambrook, J., Fritsch, E. F. and Maniatis, T., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989) (hereinafter “Maniatis”); and by Silhavy, T. J., Bennan, M. L. and Enquist, L. W., Experiments with Gene Fusions, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1984); and by Ausubel, F. M, et al., Current Protocols in Molecular Biology, published by Greene Publishing Assoc. and Wiley-Interscience (1987). Additional methods used here are in Methods in Enzymology, Volume 194, Guide to Yeast Genetics and Molecular and Cell Biology (Part A, 2004, Christine Guthrie and Gerald R. Fink (Eds.), Elsevier Academic Press, San Diego, Calif.). Other molecular tools and techniques are known in the art and include splicing by overlapping extension polymerase chain reaction (PCR) (Yu, et al. (2004) Fungal Genet. Biol. 41:973-981), positive selection for mutations at the URA3 locus of Saccharomyces cerevisiae (Boeke, J. D. et al. (1984) Mol. Gen. Genet. 197, 345-346; M A Romanos, et al. Nucleic Acids Res. 1991 Jan. 11; 19(1): 187), the cre-lox site-specific recombination system as well as mutant lox sites and FLP substrate mutations (Sauer, B. (1987) Mol Cell Biol 7: 2087-2096; Senecoff, et al. (1988) Journal of Molecular Biology, Volume 201, Issue 2, Pages 405-421; Albert, et al, (1995) The Plant Journal. Volume 7, Issue 4, pages 649-659), “seamless” gene deletion (Akada, et al. (2006) Yeast; 23(5):399-405), andgap repair methodology (Ma et al., Genetics 58:201-216; i. 981). Applicants have discovered that activation of the phosphoketolase pathway in a recombinant host cell comprising a modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity or a modification in an endogenous polypeptide having pyruvate decarboxylase activity, reduces or eliminates the need for an exogenous carbon substrate for the growth of such a cell. In embodiments, the recombinant host cells comprise (i) at least one deletion, mutation and/or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity); (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity; and optionally (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


The genetic manipulations of the host cells described herein can be performed using standard genetic techniques and screening and can be made in any host cell that is suitable to genetic manipulation (Methods in Yeast Genetic's, 2005, Cold Spring Harbor Laboratory Press Cold Spring Harbor, N.Y., pp. 201-202). In embodiments, the recombinant host cells disclosed herein can be any bacteria, yeast or fungi host useful for genetic modification and recombinant gene expression. In other embodiments a recombinant host cell can be a member of the genera Clostridium, Zymomonas, Eseherichia, Salmonella, Serratia, Erwinia, Klebsiella, Shigella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Alcaligenes, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Schizoaccharomyces, Kluyveromyces, Yarrowia, Pichia, Candida, Hansenula, Issatchenkia, saccharomyces. In other embodiments, the host cell can be Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactic, Kluyveromyces thermotolerans, Kluyveromyces marxianus, Candida glabrata, Candida albicans, Pichia stipitis, Yarrowia lipolytica, E. coli, or L. plantarum. In still other embodiments, the host cell is a yeast host cell. In some embodiments, the host cell is a member of the genera Saccharomyces. In some embodiments, the host cell is Kluyveromyces Candida glabrata or Schizosaccharomyces pombe. In some embodiments, the host cell is Saccharomyces cerevisiae. S. cerevisiae yeast are known in the art and are available from a variety of sources, including, but not limited to, American Type Culture Collection (Rockville, Md.), Centraalbureau voor Schimmelcultures (CBS) Fungal Biodiversity Centre, LeSaffre, Gert Strand AB, Ferm Solutions, North American Bioproducts, Martrex, and Lallemand. S. cerevisiae include, but are not limited to, BY4741, CEN.PK 113-7D, Ethanol Red® yeast, Ferm Pro™ yeast, Bio-Ferm® XR yeast, Gert Strand Prestige Batch Turbo alcohol yeast, Gert Strand Pot Distillers yeast, Gert Strand Distillers Turbo yeast, FerMax™ Green yeast, FerMax™ Gold yeast, Thermosacc® yeast, BG-1, PE-2, CAT-1, CBS7959, CBS7960, and CBS7961.


Sources of Acetyl-CoA


Acetyl-CoA is a major cellular building block, required for the synthesis of fatty acids, sterols, and lysine. Pyruvate is often a major contributor to the acetyl-CoA pool. Pyruvate dehydrogenase catalyzes the direct conversion of pyruvate to acetyl-CoA (E.C. 1.2.4.1, E.C. 1.2.1.51) or acetate (E.C. 1.2.2.2) and is almost ubiquitous in nature. Other enzymes involved in conversion of pyruvate to acetyl-CoA, acetyl-phosphate or acetate include pyruvate-formate lyase (E.C. 2.3.1.54), pyruvate oxidase (E.C. 1.2.3.3, E.C. 1.2.3.6), pyruvate-ferredoxin oxidoreductase (E.C. 1.2.7.1), and pyruvate decarboxylase (E.C. 4.1.1.1). Genetic modifications made to a host cell to conserve the pyruvate pool for a product of interest may include those that restrict conversion to acetyl-CoA, leading to decreased growth in the absence of an exogenously supplied two-carbon substrate, a carbon substrate that can be readily converted to acetyl-CoA independent of pyruvate (e.g. ethanol or acetate). An example is the documented auxotrophy observed in pyruvate decarboxylase deficient Saccharomyces cerevisiae (Flikweert et al. 1999, supra). Another example is the documented auxotrophy observed in pyruvate dehydrogenase deficient Escherichia coli when grown aerobically on glucose (Langley and Guest, 1977, J. Gen. Microbial. 99:2630276).


Modification of Pyruvate Decarboxylase

In embodiments, the recombinant host cells disclosed herein comprise a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase (PDC) or a modification in an endogenous polypeptide having PDC activity. In embodiments, the recombinant host cells disclosed herein can have a modification or disruption of one or more polynucleotides, genes or polypeptides encoding PDC. In embodiments, the recombinant host cell comprises at least one deletion, mutation, and/or substitution in one of more endogenous polynucleotides or genes encoding a polypeptide having PDC activity, or in one or more endogenous polypeptides having PDC activity. Such modifications, disruptions, deletions, mutations, and/or substitutions can result in PDC activity that is reduced or eliminated, resulting in a PDC knock-out (PDC-KO) phenotype.


In embodiments, the endogenous pyruvate decarboxylase activity of the recombinant host cells disclosed herein converts pyruvate to acetaldehyde, which can then be converted to ethanol or to acetyl-CoA via acetate.


In embodiments, the recombinant host cell is Kluyveromyces lactis containing one gene encoding pyruvate decarboxylase, Candida glabrata containing one gene encoding pyruvate decarboxylase, or Schizosaccharomyces pombe containing one gene encoding pyruvate decarboxylase.


In other embodiments, the recombinant host cell is Saccharomyces cerevisiae containing three isozymes of pyruvate decarboxylase encoded by the pdc1, pdc5, and pdc6 genes, as well as a pyruvate decarboxylase regulatory gene, pdc2. In a non-limiting example in S. cerevisiae, the pdc1 and pdc5 genes, or all three genes, are disrupted. In another non-limiting example in S. cerevisiae, pyruvate decarboxylase activity may be reduced by disrupting the pdc2 regulatory gene. In, another non-limiting example in S. cerevisiae, polynucleotides or genes encoding pyruvate decarboxylase proteins such as those having about 70% to about 75%, about 75% to about 80%, about 80% to about 85%, about 85% to about 90%, about 90% to about 95%, about 96%, about 97%, about 98%, or about 99% sequence identity to pdc1 or pdc5 can be disrupted.


In embodiments, the polypeptide having PDC activity or the polynucleotide or gene encoding a polypeptide having PDC activity is associated with Enzyme Commission Number EC 4.1.1.1. In other embodiments, a PDC gene of the recombinant host cells disclosed herein is not active under the fermentation conditions used, and therefore such a gene would not need to be modified or inactivated.


Examples of recombinant host cells with reduced pyruvate decarboxylase activity due to disruption of pyruvate decarboxylase encoding genes have been reported, such as for Saccharomyces in Flikweert et al. (Yeast (1996) 12:247-257), for Kluyveromyces in Bianchi et al. (Mol. Microbiol. (1996) 19(1):27-36), and disruption of the regulatory gene in Hohmann (Mol. Gen. Genet. (1993) 241:657-666). Saccharomyces strains having no pyruvate decarboxylase activity are available from the ATCC with Accession #200027 and #200028.


Examples of PDC polynucleotides, genes and polypeptides that can be targeted for modification or inactivation in the recombinant host cells disclosed herein include, but are not limited to, those of the following table.









TABLE 3







SEQ ID NOs of pyruvate decarboxylase (PDC)


target gene coding regions and proteins.












SEQ ID NO:
SEQ ID NO:



Description
Nucleic acid
Amino acid















PDC1 pyruvate decarboxylase
1
2



from Saccharomyces cerevisiae



PDC5 pyruvate decarboxylase
3
4



from Saccharomyces cerevisiae



PDC6 pyruvate decarboxylase
5
6



from Saccharomyces cerevisiae



pyruvate decarboxylase from
7
8




Candida glabrata




PDC1 pyruvate decarboxylase
9
10



from Pichia stipitis



PDC2 pyruvate decarboxylase
11
12



from Pichia stipitis



pyruvate decarboxylase from
13
14




Kluyveromyces lactis




pyruvate decarboxylase from
15
16




Yarrowia lipolytica




pyruvate decarboxylase from
17
18




Schizosaccharomyces pombe




pyruvate decarboxylase from
18
20




Zygosaccharomyces rouxii











Other examples of PDC polynucleotides, genes and polypeptides that can be targeted for modification or inactivation in the recombinant host cells disclosed herein include, but are not limited to, PDC polynucleotides, genes and/or polypeptides having at least about 70% to about 75%, about 75% to about 80%, about 80% to about 85%, about 85% to about 90%, about 90% to about 95%, about 96%, about 97%, about 98%, or about 99% sequence identity to any one of the sequences of Table 3.


In embodiments, the sequences of other PDC polynucleotides, genes and/or polypeptides can be identified in the literature and in bioinformatics databases well known to the skilled person using sequences disclosed herein and available in the art. For example, such sequences can be identified through BLAST (as described above) searching of publicly available databases with known PDC encoding polynucleotide or polypeptide sequences. In such a method, identities can be based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.


Additionally, the PDC polynucleotide or polypeptide sequences described herein or known the art can be used to identify other PDC homologs in nature. For example, each of the PDC encoding nucleic acid fragments described herein can be used to isolate genes encoding homologous proteins. Isolation of homologous genes using sequence-dependent protocols is well known in the art. Examples of sequence-dependent protocols include, but are not limited to (1) methods of nucleic acid hybridization; (2) methods of DNA and RNA amplification, as exemplified by various uses of nucleic acid amplification technologies [e.g., polymerase chain reaction (PCR), Mullis et al., U.S. Pat. No. 4,683,202; ligase chain reaction (LCR), Tabor, S. et al., Proc. Acad. Sci. USA 82:1074 (1985); or strand displacement amplification (SDA), Walker et al., Proc. Natl. Acad. Sci. USA., 89:392 (1992)]; and (3) methods of library construction and screening by complementation.


In embodiments, PDC polynucleotides, genes and/or polypeptides related to the recombinant host cells described herein can be modified or disrupted. Many methods for genetic modification and disruption of target genes to reduce or eliminate expression are known to one of ordinary skill in the art and can be used to create the recombinant host cells described herein. Modifications that can be used include, but are not limited to, deletion of the entire gene or a portion of the gene encoding a PDC protein, inserting a DNA fragment into the encoding gene (in either the promoter or coding region) so that the protein is not expressed or expressed at lower levels, introducing a mutation into the coding region which adds a stop codon or frame shift such that a functional protein is not expressed, and introducing one or more mutations into the coding region to alter amino acids so that a non-functional or a less active protein is expressed. In other embodiments, expression of a target gene can be blocked by expression of an antisense RNA or an interfering RNA, and constructs can be introduced that result in, cosuppression. In other embodiments, the synthesis or stability of the transcript can be lessened by mutation. In embodiments, the efficiency by which a protein is translated from mRNA can be modulated by mutation. All of these methods can be readily practiced by one skilled in the art making use of the known or identified sequences encoding target proteins.


In other embodiments, DNA sequences surrounding a target PDC coding sequence are also useful in some modification procedures and are available, for example, for yeasts such as Saccharomyces cerevisiae in the complete genome sequence coordinated by Genome Project ID9518 of Genome Projects coordinated by NCBI (National Center for Biotechnology Information) with identifying GOPID #413838. An additional non-limiting example of yeast genomic sequences is that of Candida albicans, which is included in GPID #10771, #10701 and #416373, Other yeast genomic sequences can be readily found by one of skill in the art in publicly available databases.


In other embodiments, DNA sequences surrounding a target PDC coding sequence can be useful for modification methods using homologous recombination. In a non-limiting example of this method, PDC gene flanking sequences can be placed bounding a selectable marker gene to mediate homologous recombination whereby the marker gene replaces the PDC gene. In another non-limiting example, partial PDC gene sequences and PDC, gene flanking sequences bounding a selectable marker gene can be used to mediate homologous recombination whereby the marker gene replaces a portion of the target PDC, gene. In embodiments, the selectable marker can be bounded by site-specific recombination sites, so that following expression of the corresponding site-specific recombinase, the resistance gene is excised from the PDC gene without reactivating the latter. In embodiments, the site-specific recombination leaves behind a recombination site which disrupts expression of the PDC protein. In other embodiments, the homologous recombination vector can be constructed to also leave a deletion in the PDC gene following excision of the selectable marker, as is well known to one skilled in the art.


In other embodiments, deletions can be made to a PDC target gene using mitotic recombination as described in Wach et al. (Yeast, 10:1793-1808; 1994). Such a method can involve preparing a DNA fragment that contains a selectable marker between genomic regions that can be as short as 20 bp, and which bound a target DNA sequence. In other embodiments, this DNA fragment can be prepared by PCR amplification of the selectable marker gene using as primers oligonucleotides that hybridize to the ends of the marker gene and that include the genomic regions that can recombine with the yeast genome. In embodiments, the linear DNA fragment can be efficiently transformed into yeast and recombined into the genome resulting in gene replacement including with deletion of the target DNA sequence ((as described, for example, in Methods in Enzymology, Volume 194, Guide to Yeast Genetics and Molecular and Cell Biology (Part A. 2004, Christine Guthrie and Gerald R. Fink (Eds.), Elsevier Academic Press, San Diego, Calif.)).


Moreover, promoter replacement methods can be used to exchange the endogenous transcriptional control elements allowing another means to modulate expression such as described in Mnaimneh et al. ((2004) Cell 118(1):31-44).


In other embodiments, the PDC target gene encoded activity can be disrupted using random mutagenesis, which can then be followed by screening to identify strains with dependency on carbon substrates for growth. In this type of method, the DNA sequence of the target gene encoding region, or any other region of the genome affecting carbon substrate dependency for growth, need not be known. In embodiments, a screen for cells with reduced PDC activity and/or two-carbon substrate dependency, or other mutants having reduced PDC activity and a reduced or eliminated dependency for exogenous two-carbon substrate for growth, can be useful as recombinant host cells of the invention.


Methods for creating genetic mutations are common and well known in the art and can be applied to the exercise of creating mutants. Commonly used random genetic modification methods (reviewed in Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.) include spontaneous mutagenesis, mutagenesis caused by mutator genes, chemical mutagenesis, irradiation with UV or X-rays, or transposon mutagenesis.


Chemical mutagenesis of host cells can involve, but is not limited to, treatment with one of the following DNA mutagens: ethyl methanesulfonate (EMS), nitrous acid, diethyl sulfate, or N-methyl-N′-nitro-N-nitroso-guanidine (MNNG). Such methods of mutagenesis have been reviewed in Spencer et al., (Mutagenesis in Yeast, 1996, Yeast Protocols: Methods in Cell and Molecular Biology. Humana Press, Totowa, N.J.). In embodiments, chemical mutagenesis with EMS can be performed as described in Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. Irradiation with ultraviolet (UV) light or X-rays can also be used to produce random mutagenesis in yeast cells. The primary effect of mutagenesis by UV irradiation is the formation of pyrimidine dimers which disrupt the fidelity of DNA replication. Protocols for UV-mutagenesis of yeast can be found in Spencer et al. (Mutagenesis in Yeast, 1996, Yeast Protocols: Methods in Cell and Molecular Biology. Humana Press, Totowa, N.J.). In embodiments, the introduction of a mutator phenotype can also be used to generate random chromosomal mutations in host cells. In embodiments, common imitator phenotypes can be obtained through disruption of one or more of the following genes: PMS1, MAG1, RAD18 or RAD51. In other embodiments, restoration of the non-mutator phenotype can be obtained by insertion of the wildtype allele. In other embodiments, collections of modified cells produced from any of these or other known random mutagenesis processes may be screened for reduced or eliminated PDC activity.


Genomes have been completely sequenced and annotated and are publicly available for the following yeast strains: Ashbya gossypii ATCC 10895, Candida glabrata CBS138, Kluyveromyces lactis NRRL Y-1140, Pichia stipitis CBS 6054, Saccharomyces cerevisiae S288c, Schizosaccharomyces pombe 972h-, and Yarrowia lipolytica CLIB 122. Typically BLAST (described above) searching of publicly available databases with known PDC polynucleotide or polypeptide sequences, such as those provided herein, is used to identify PDC-encoding sequences of other host cells, such as yeast cells.


Accordingly, it is within the scope of the invention to provide pyruvate decarboxylase polynucleotides and polypeptides having at, least about 70% to about 75%, about 75% to about 80%, about 80% to about 85%, about 85% to about 90%, about 90% to about 95%, about 96%, about 97%, about 98%, or about 99% sequence identity to any of the PDC polypeptides or polypeptides disclosed herein (SEQ ID NOs: 1-20). Identities are based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.


The modification of PDC in the host cells disclosed herein to reduce or eliminate PDC activity can be confirmed using methods known in the art. For example, PCR methods well known in the art can be used to confirm deletion of PDC. Other suitable methods will be known to those of skill in the art and include, but are not limited to lack of growth on yeast extract peptone-dextrose medium (YPD).


Introduction of the Phosphokeetolase Pathway

Applicants have found that expression of enzymes associated with the phosphoketolase pathway (e.g., phosphoketolase and/or phosphotransacetylase) results in a reduced or eliminated requirement for exogenous two-carbon substrate supplementation for growth of PDC-KO cells. Phosphoketolases and/or phosphotransacetylases identified as described herein, can be expressed in such cells using methods described herein.


Enzymes of the phosphoketolase pathway include phosphoketolase and phosphotransacetylase (FIG. 1). Phosphoketolase (Enzyme Commission Number EC 4.1.2.9) catalyzes the conversion of xylulose. 5-phosphate into glyceraldehyde 3-phosphate and acetyl-phosphate (heath et al., J. Biol. Chem. 231: 1009-29; 1958). Phosphoketolase activity has been identified in several yeast strains growing with xylose as the sole carbon source but not in yeast strains grown with glucose (Evans and Ratledge; Arch. Microbiol. 139: 48-52; 1984). Inhibitors of phosphoketolase include, but are not limited to, erythrose 4-phosphate and glyceraldehyde 3-phosphate. Phosphotransacetylase (Enzyme Commission Number EC 2.3.1.8) converts acetyl-phosphate into acetyl-CoA.


In embodiments, the phosphoketolase pathway is activated in the recombinant host cells disclosed herein by engineering the cells to express polynucleotides and/or polypeptides encoding phosphoketolase and, optionally, phosphotransacetylase. In embodiments, the recombinant host cells disclosed herein comprise a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. In other embodiments, the recombinant host cells disclosed herein comprise a heterologous polynucleotide encoding, a polypeptide having phosphoketolase activity and a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. In other embodiments, the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity is overexpressed, or expressed at a level that is higher than endogenous expression of the same or related endogenous gene, if any. In still other embodiments, the heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity is overexpressed, or expressed at a level that is higher than endogenous expression of the same or related endogenous gene, if any.


In embodiments, a polypeptide having phosphoketolase activity catalyzes the conversion of xylulose 5-phosphate into glyceraldehyde-3-phosphate and acetyl-phosphate and/or the conversion of fructose-6-phosphate into erythrose-4-phosphate and acetyl-phosphate. In embodiments, the activity of a polypeptide having phosphoketolase activity is inhibited by erythrose 4-phosphate and/or glyceraldehyde 3-phosphate. In other embodiments, a polypeptide having phosphotransacetylase activity catalyzes the conversion of acetyl-phosphate into acetyl-CoA.


Numerous examples of polynucleotides, genes and polypeptides encoding phosphoketolase activity are known in the art and can be used in the recombinant host cells disclosed herein. In embodiments, such a polynucleotide, gene and/or polypeptide can be the xylulose 5-phosphateketolase (XpkA) of Lactobacillus pentosus MD363 (Posthuma et al., Appl. Environ Microbiol. 68: 831-7; 2002). XpkA is the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and exhibits a specific activity of 4.455 μmol/min/mg (Posthuma et al., Appl. Environ. Microbiol. 68: 831-7; 2002). In other embodiments, such a polynucleotide, gene and/or polypeptide can be the phosphoketolase of Leuconostoc mesenteroides which exhibits a specific activity of 9.9 μmol/min/mg and is stable at pH above 4.5 (Goldberg et al., Methods Enzymol. 9: 515-520; 1966). This phosphoketolase exhibits a Km of 4.7 mM for D-xylulose 5-phosphate and a Km of 29 mM for fructose 6-phosphate (Goldberg et al., Methods Enzymol. 9: 515-520; 1966). In other embodiments, such a polynucleotide, gene and/or polypeptide can be the D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase gene xfp from B. lactis, as described, for example, in a pentose-metabolizing S. cerevisiae strain by Sonderegger et al. (Appl. Environ. Microbiol. 70: 2892-7; 2004).


In embodiments, a polynucleotide, gene and/or polypeptide encoding phosphoketolase corresponds to the Enzyme Commission Number EC 4.1.2.9.


In embodiments, host cells comprise a polypeptide having at least about 80%, at least about 85%, at least about 90%, or 100% identity to a polypeptide of Table 4 or an, active fragment thereof or a polynucleotide encoding such a polypeptide. In other embodiments, a polynucleotide, gene and/or polypeptide encoding phosphoketolase can include, but is not limited to, a sequence provided in the following tables 4 or 5.









TABLE 4







SEQ ID NOs of phosphoketolase target gene coding regions and proteins











SEQ ID
SEQ ID




NO:
NO:




Nucleic
Amino



Description
acid
acid
Amino Acid sequence













Xpk1
172
481
MTTDYSSPAYLQKVDKYWRAANYLSVGQLYLKDNPLLQRPL


phospho-


KASDVKVHPIGHWGTIAGQ


ketolase from


NFIYAHLNRVINKYGLKMFYVEGPGHGGQVMVSNSYLDGTY



Lactobacillus



TDIYPEITQDVEGMQKLFK



plantarum



QFSFPGGVASHAAPETPGSIHEGGELGYSISHGVGAILDNP





DEIAAVVVGDGESETGPLA





TSWQSTKFINPINDGAVLPILNLNGFKISNPTIFGRTSDAK





IKEYFESMNWEPIFVEGDD





PEKVHPALAKAMDEAVEKIKAIQKHARENNDATLPVWPMIV





FRAPKGWTGPKSWDGDKIE





GSFRAHQIPIPVDQNDMEHADALVDWLESYQPKELFNEDGS





LKDDIKEIIPTGDSRMAAN





PITNGGVDPKALNLPNFRDYAVDTSKEGANVKQDMIVWSDY





LRDVIKKNPDNFRLFGPDE





TMSNRLYGVFETTNRQWMEDIHPDSDQYEAPAGRVLDAQLS





EHQAEGWLEGYVLTGRHGL





FASYEAFLRVVDSMLTQHFKWLRKANELDWRKKYPSLNIIA





ASTVFQQDHNGYTHQDPGA





LTHLAEKKPEYIREYLPADANTLLAVGDVIFRSQEKINYVV





TSKHPRQQWFSIEEAKQLV





DNGLGIIDWASTDQGSEPDIVFAAAGTEPTLETLAAIQLLH





DSFPEMKIRFVNVVDILKL





RSPEKDPRGLSDAEFDHYFTKDKPVVFAFHGYEDLVRDIFF





DRHNHNLYVHGYRENGDIT





TPFDVRVMNQMDRFDLAKSAIAAQPAMENTGAAFVQSMDNM





LAKHNAYIRDAGTDLPEVN





DWQWKGLK





XpkA
1890
1889
MSTDYSSPAYLQKVDKYWRAANYLSVGQLYLKDNPLLQRPL


phospho-


KASDVKVHPIGHWGTIAGQ


ketolase from


NFIYAHLNRVINKYGLKMFYVEGPGHGGQVMVSNSYLDGTY



Lactobacillus



TDIYPEITQDVEGMQKLFK



pentosus



QFSFPGGVASHAAPETPGSIHEGGELGYSISHGVGAILDNP


MD363


DEIAAVVVGDGESETGPLA





TSWQSTKFINPINDGAVLPILNLNGFKISNPTIFGRTSDEK





IKQYFESMNWEPIFVEGDD





PEKVHPALAKAMDEAVEKIKAIQKNARENDDATLPVWPMIV





FRAPKGWTGPKSWDGDKIE





GSFRAHQIPIPVDQTDMEHADALVDWLESYQPKELFNEDGS





LKDDIKEIIPTGDARMAAN





PITNGGVDPKALNLPNFRDYAVDTSKHGANVKQDMIVWSDY





LRDVIKKNPDNFRLFGPDE





TMSNRLYGVFETTNRQWMEDIHPDSDQYEAPAGRVLDAQLS





EHQAEGWLEGYVLTGRHGL





FASYEAFLRVVDSMLTQHFKWLRKANELDWRKKYPSLNIIA





ASTVFQQDHNGYTHQDPGA





LTHLAEKKPEYIREYLPADANSLLAVGDVIFRSQEKINYVV





TSKHPRQQWFSIEEAKQLV





DNGLGIIDWASTDQGSEPDIVFAAAGTEPTLETLAAIQLLH





DSFPDMKIRFVNVVDILKL





RSPEKDPRGLSDAEFDHYFTKDKPVVFAFHGYEDLVRDIFF





DRHNHNLHVHGYRENGDIT





TPFDVRVMNQMDRFDLAKSAIAAQPAMENTGAAFVQDMDNM





LAKHNAYIRDAGTDLPEVN





DWQWKGLK





Xpf D-xylulose
79
388
MTNPVIGTPWQKLDRPVSEEAIEGMDKYWRVANYMSIGQIY


5-phosphate/D-


LRSNPLMKEPFTRDDVKHR


fructose 6-


LVGHWGTTPGLNFLLAHINRLIADHQQNTVFIMGPGHGGPA


phosphate


GTAQSYIDGTYTEYYPNIT


phospho-


KDEAGLQKFFRQFSYPGGIPSHFAPETPGSIHEGGELGYAL


ketolase


SHAYGAIMDNPSLFVPCII


from B. lactis


GDGEAETGPLATGWQSNKLVNPRTDGIVLPILHLNGYKIAN





PTILARISDEELHDFFRGM





GYHPYEFVAGFDNEDHLSIHRRFAELFETIFDEICDIKAAA





QTDDMTRPFYPMLIFRTPK





GWTCPKFIDGKKTEGSWRAHQVPLASARDTEAHFEVLKGWM





ESYKPEELFNADGSIKEDV





TAFMPKGELRIGANPNANGGRIREDLKLPELDQYEITGVKE





YGHGWGQVEAPRSLGAYCR





DIIKNNPDSFRVFGPDETASNRLNATYEVTKKQWDNGYLSA





LVDENMAVTGQVVEQLSEH





QCEGFLEAYLLTGRHGIWSSYESFVHVIDSMLNQHAKWLEA





TVREIPWRKPISSVNLLVS





SHVWRQDHNGFSHQDPGVTSVLLNKTFNNDHVTNIYFATDA





NMLLAIAEKCFKSTNKINA





IFAGKQPAATWITLDEARAELEAGAAEWKWASNAKSNDEVQ





VVLAAAGDVPTQEIMAASD





ALNKMGIKFKVVNVVDLIKLQSSKENDEAMSDEDFADLFTA





DKPVLFAYHSYAQDVRGLI





YDRPNHDNFTVVGYKEQGSTTTPFDMVRVNDMDRYALQAKA





LELIDADKYADKINELNEF





RKTAFQFAVDNGYDIPEFTDWVYPDVKVDETSMLSATAATA





GDNE









In other embodiments, a polynucleotide, gene and/or polypeptide encoding phosphoketolase can have at least about 70% to about 75%, about 75% to about 80%, about 80% to about 85%, about 85% to about 90%, about 90% to about 95%, about 96%, about 97%, about 98%, or about 99% sequence identity to that of any one of the sequences of Table 4, wherein the polynucleotide, gene and/or polypeptide encodes a polypeptide having phosphoketolase activity.


In other embodiments, a polynucleotide, gene and/or polypeptide encoding phosphoketolase can be used to identify other phosphoketolase polynucleotide, gene and/or polypeptide sequences or to identify phosphoketolase homologs in other cells, as described above for PDC. Such phosphoketolase encoding sequences can be identified, for example, in, the literature and/or in bioinformatics databases well known to the skilled person. For example, the identification of phosphoketolase encoding sequences in other cell types using bioinformatics can be accomplished through BLAST (as described above) searching of publicly available databases with known phosphoketolase encoding DNA and polypeptide sequences, such as those provided herein. Identities are based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.


Additional phosphoketolase target gene coding regions were identified using, diversity search, clustering, experimentally verified xylulose-5-phosphate/fructose-6-phosphate phosphoketolases and domain architecture. Briefly, a BLAST search with the experimentally verified sequences with an Evalue cut-off of 0.01 resulted in 595 sequence matches. Clustering with the CD-HIT program at 95% sequence identity and 90% length overlap reduced the number to 436. CD-HIT is a program for clustering large protein database at nigh sequence identity threshold. The program removes redundant sequences and gel erates a database of only the representatives. (Clustering of highly homologous sequences to reduce the size of large protein database, Weizhong Li, Lukasz Jaroszewski & Adam Godzik Bioinformatics, (2001) 17:282-283)


Xylulose-5-phosphate/fructose-6-phosphate phosphoketolases have three Pfam domains: XFP_XFP; XFP_C. Although each of these domains may be present in several domain architectures, e.g. XFP_N is found in eight architectures. The architecture of interest was determined to be XFP_N; XFP; XFP_C. The cumulative length of the three domains is 760 amino acids.


A structure/function characterization of the phosphoketolases was performed using the HMMER software package. The following information based on the HMMER software user guide gives some description of the way that the hmmbuild program prepares a Profile HMM. A Profile HMM is capable of modeling gapped alignments, e.g. including insertions and deletions, which lets the software describe a complete conserved domain (rather than just a small ungapped motif). Insertions and deletions are modeled using insertion (I) states and deletion (D) states. All columns that contain more than a certain fraction x of gap characters will be assigned as an insert column. By default, x is set to 0.5. Each match state has an I and a D state associated with it. HMMER calls a group of three states (M/D/I) at the same consensus position in the alignment a “node”, These states are interconnected with arrows called state transition probabilities. M and I states are emitters, while D states are silent. The transitions are arranged so that at each node, either the M state is used (and a residue is aligned and scored) or the D state is, used (and no residue is aligned, resulting in a deletion-gap character, ‘-’). Insertions occur between nodes, and I states, have a self-transition, allowing one or more inserted residues to occur between consensus columns.


The scores of residues in a match state (i.e. match state emission scores), or in an insert state (i.e. insert state emission scores) are proportional to Log2 (p_x)/(null_x). Where p_x is the probability of an amino acid residue, at a particular position in the alignment, according to the Profile HMM and null_x is the probability according to the Null model. The Null model is a simple one state probabilistic model with pre-calculated set of emission probabilities for each of the 20 amino acids derived from the distribution of amino acids in the SWISSPROT release 24. State transition scores are also calculated as log odds parameters and are propotional to Log2 (t_x). Where t_x is the probability of transiting to an emitter or non-emitter state.


Using a multiple sequence alignment of experimentally verified sequences containing the architecture of interest XFP_N; XFP; XFP_C, a profile Hidden Markov Model (HMM) was created for representing members of the xylulose-5-phosphate/fructose-6-phosphate phosphoketolases (XPK-XFP). As stated in the user guide, Profile HMMs are statistical models of multiple sequence alignments. They capture position-specific information about how conserved each column of the alignment is, and which amino acid residues are most likely to occur at each position. Thus HMMs have a formal probabilistic basis. Profile HMMs for a large number of protein families are publicly available in the PFAM database (Janelia Farm Research Campus, Ashburn, Va.), see ftp://ftp.sanger.ac.uk/pub/databases/Pfam/releases/Pfam24.0/.


Eight xylulose-5-phosphate/fructose-6-phosphate phosphoketolases sequences with experimentally verified function were identified in the BRENDA database:


1. CBF76492.1 from Aspergillus nidulans FGSC A4 (SEQ ID NO: 355)


2. AAR98787.1 from Bifidobacterium longum (SEQ ID NO: 379)


3. ZP03646196.1 from Bifidobacterium bifidum NCIMB 41171 (SEQ ID NO: 381)


4. ZP02962870.1 from Bifidobacterium animalis subsp. lactis HNO19 (SEQ ID NO: 388)


5. ZP786060.1 from Lactobacillus plantarum WCFS1 (SEQ ID NO: 481)


6. ZP03940142.1 from Lactobacillus brevis subsp. gravesensis ATCC 27305 (SEQ ID NO: 486)


7. ZP03073172.1 from Lactobacillus reuteri 100-23 (SEQ ID NO 468)


8. YP818922.1 from Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 (SEQ ID NO: 504)


The BRENDA database is a freely available information system containing biochemical and molecular information on all classified enzymes as well as software tools for querying the database and calculating molecular properties. The database covers information on classification and nomenclature, reaction and specificity, functional parameters, occurrence, enzyme structure and stability, mutants and enzyme engineering, preparation and isolation, the application of enzymes, and ligand-related data. (BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009, Nucleic Acids Res. 2009 Jan; 37 (Database issue): D588-92. Epub 2008 Nov. 4. Chang A. Scheer M, Grote A, Schomburg 1.Schomburg D.) The eight sequences were used to build a profile HMM which is provided herein as Table 6.


To further identify the proteins of interest, the 436 sequences were searched with tour profile HMMs: the generated XPK_XFP_HMM profile HMM provided in Table 6 as well as the three published profiles for the three domains XFP_N; XFP_C (PFAM DATABASE) described in Tables 7, 8, and 9, respectively. 309 protein sequences which lengths were between 650 amino acids and 900 amino acids, and contained the three domains were retained.


All 309 sequences are at least 40% identical to an experimentally verified phosphoketolase, with exception of 12 sequences that are within 35% identity distance. However, all 31.9 sequences have a highly significant match to all 4 profile HMMs. The least significant matches have Evalues of 7.5E-242, 1,1E-124, 2,1E-49, 7.8E-37 to XFP_XPK HMM, XFP_N, XFP, and XFP_C profile HMMs respectively. The 309 sequences are provided in Table 5, however, it is understood that any xylulose-5-phosphate/fructose-6-phosphate phosphoketolase identifiable by the method described may be expressed in host cells as described herein. Where accession information is given as “complement (NN_NNNNN:X . . . Y)”, it should be understood to mean the reverse complement of nucleotides X to Y of the sequence with Accession number NN_NNNNN.N. Where accession information is given as “join (NNNNNN.N:X . . . Y, NNNNNN.N:Z . . . Q)”, it should be understood to mean the sequence resulting from joining nucleotides X to Y of NNNNNN.N to nucleotides Z to Q of NNNNNN.N.









TABLE 5







SEQ ID NOs of xylulose-5-phosphate/fructose-6-phosphate


phosphoketolase target gene coding regions and proteins.













GENBANK
Nucleic
GENBANK
Amino




Nucleotide
Acid
Amino Acid
Acid


GI
Sequence
SEQ
Sequence
SEQ
Source


Number
Accession Information
ID NO:
Accession No.
ID NO:
Organism















162147402
complement
21
YP_001601863.1
330

Gluconacetobacter




(NC_010125.1:




diazotrophicus




1624982 . . . 1627414)



PAl 5


127512024
complement
22
YP_001093221.1
331

Shewanella




(NC_009092.1:




loihica




1256548 . . . 1258914)



PV-4


119774052
complement
23
YP_926792.1
332

Shewanella




(NC_008700.1:




amazonensis




1105589 . . . 1107955)



SB2B


113971300
NC_008321.1:
24
YP_735093.1
333

Shewanella




3541276 . . . 3543642



sp. MR-4


126173290
complement
25
YP_001049439.1
334

Shewanella




(NC_009052.1:




baltica




1204351 . . . 1206717



OS155


163750647
complement
26
ZP_02157884.1
335

Shewanella




(NZ_ABIC01000018.1:




benthic
text missing or illegible when filed




47875 . . . 50253)



KT99


157374325
complement
27
YP_001472925.1
336

Shewanella




(NC_009831.1:




sediminis




1410491 . . . 1412857)



HAW-EB3


170725643
complement
28
YP_001759669.1
337

Shewanella




(NC_010506.1:




woodyi




1624098 . . . 1626464)



ATCC 51908


167623058
complement
29
YP_001673352.1
338

Shewanella




(NC_010334.1:




halifaxensis




1364332 . . . 1366698)



HAW-EB4


91794082
NC_007954.1:
30
YP_563733.1
339

Shewanella




3270590 . . . 3272956




denitrificans








OS217


254498997
NZ_ACUL01000224.1:
31
ZP_05111697.1
340

Legionella




8243 . . . 10492




drancourtii








LLAP12


239607320
join(EQ999973.1:
32
EEQ84307.1
341

Ajellomyces




7995808 . . . 7995885,




dermatitidis




EQ999973.1:



ER-3



7996059 . . . 7996116,



EQ999973.1:



7996178 . . . 7996412,



EQ999973.1:



7996486 . . . 7996638,



EQ999973.1:



7996791 . . . 7997016,



EQ999973.1:



7997082 . . . 7997548,



EQ999973.1:



7997603 . . . 7997705,



EQ999973.1:



7997779 . . . 7998604,



EQ999973.1:



7998677 . . . 7998717)


261200667
XM_002626688.1:
33
XP_002626734.1
342

Ajellomyces




1 . . . 2034




dermatitidis








SLH14081


154276328
XM_001538959.1:
34
XP_001539009.1
343

Ajellomyces




1 . . . 2421




capsulatus








NAm1


225555843
join(GG663374.1:
35
EEH04133.1
344

Ajellomyces




72330 . . . 72492,




capsulatus




GG663374.1:



G186AR



72695 . . . 72929,



GG663374.1:



73005 . . . 73157,



GG663374.1:



73216 . . . 74203,



GG663374.1:



74274 . . . 75146)


225681974
join(DS544805.1:
36
EEH20258.1
345

Paracoccidioides




2187102 . . . 2187264,




brasiliensis




DS544805.1:



Pb03



2187353 . . . 2187427,



DS544805.1:



2187521 . . . 2187755,



DS544805.1:



2187839 . . . 2187991,



DS544805.1:



2188086 . . . 2188395,



DS544805.1:



2188462 . . . 2189082,



DS544805.1:



2189147 . . . 2189263,



DS544805.1:



2189358 . . . 2189973,



DS544805.1:



2190044 . . . 2190084)


226289140
join(DS572750.1:
37
EEH44652.1
346

Paracoccidioides




3105630 . . . 3105792,




brasiliensis




DS572750.1:



Pb18



3106049 . . . 3106283,



DS572750.1:



3106367 . . . 3106519,



DS572750.1:



3106614 . . . 3106923,



DS572750.1:



3107023 . . . 3107610,



DS572750.1:



3107675 . . . 3108500,



DS572750.1:



3108572 . . . 3108612)


258564014
XM_002582706.1:
38
XP_002582752.1
347

Uncinocarpus




1 . . . 2421




reesii








1704


240108203
join(ACFW01000030.1:
39
EER26377.1
348

Coccidioides




1281918 . . . 1282080,




posadasii




ACFW01000030.1:



C735 delttext missing or illegible when filed  SOWgp



1282133 . . . 1282207,



ACFW01000030.1:



1282266 . . . 1282500,



ACFW01000030.1:



1282551 . . . 1282703,



ACFW01000030.1:



1282757 . . . 1283066,



ACFW01000030.1:



1283132 . . . 1283752,



ACFW01000030.1:



1283828 . . . 1284653,



ACFW01000030.1:



1284726 . . . 1284763)


238838423
join(DS995701.1:
40
EEQ28085.1
349

Microsporum




2675053 . . . 2675215,




canis




DS995701.1:



CBS 113480



2675278 . . . 2675352,



DS995701.1:



2675424 . . . 2675658,



DS995701.1:



2675745 . . . 2675897,



DS995701.1:



2675972 . . . 2676281,



DS995701.1:



2676341 . . . 2676961,



DS995701.1:



2677062 . . . 2677836,



DS995701.1:



2677864 . . . 2677933,



DS995701.1:



2677998 . . . 2678013)


169770631
XM_001819733.1:
41
XP_001819785.1
350

Aspergillus




1 . . . 2457




oryzae








RIB40


145232813
XM_001399743.1:
42
XP_001399780.1
351

Aspergillus




1 . . . 2421




niger



119491775
XM_001263381.1:
43
XP_001263382.1
352

Neosartorya




1 . . . 2421




fischer
text missing or illegible when filed








NRRL 181


121705634
XM_001271079.1:
44
XP_001271080.1
353

Aspergillus




1 . . . 2421




clavatu
text missing or illegible when filed








NRRL 1


115396290
XM_001213784.1:
45
XP_001213784.1
354

Aspergillus




1 . . . 2400




terreus








NIH2624


259482219
join(BN001303.1:
46
CBF76492.1
355

Aspergillus




576345 . . . 576507,




nidulan
text missing or illegible when filed




BN001303.1:



FGSC A4



576696 . . . 576930,



BN001303.1:



576981 . . . 577133,



BN001303.1:



577185 . . . 577494,



BN001303.1:



577544 . . . 578161,



BN001303.1:



578210 . . . 579035,



BN001303.1:



579091 . . . 579128)


255942289
XM_002561867.1:
47
XP_002561913.1
356

Penicillium




1 . . . 2469




chrysogenum








Wisconsin 54-1255


242784458
XM_002480346.1:
48
XP_002480391.1
357

Talaromyces




69 . . . 2489




stipitatus








ATCC 10500


212527714
XM_002143978.1:
49
XP_002144014.1
358

Penicillium




139 . . . 2559




marneffei








ATCC 18224


70999652
XM_749450.1:
50
XP_754543.1
359

Aspergillus




1 . . . 2145




fumigatus








Af293


154314622
XM_001556585.1:
51
XP_001556635.1
360

Botryotinia




1 . . . 2061




fuckeliana








B05.10


156053245
XM_001592499.1:
52
XP_001592549.1
361

Sclerotinia




1 . . . 2430




sclerotiorum








1980


46124351
XM_386729.1:
53
XP_386729.1
362

Gibberella




1 . . . 2418




zeae








PH-1


256733824
complement
54
EEU47171.1
363

Nectria




(join(GG698897.1:




haematococca




220636 . . . 220670,



mpV 77-13-4



GG698897.1:



220723 . . . 221650,



GG698897.1:



221704 . . . 222510,



GG698897.1:



222576 . . . 222732,



GG698897.1:



222783 . . . 223020,



GG698897.1:



223072 . . . 223146,



GG698897.1:



223199 . . . 223361))


261354209
join(DS985216.1:
55
EEY16637.1
364

Verticillium




747889 . . . 748126,




albo-atrum




DS985216.1:



VaMs. 102



748174 . . . 748564,



DS985216.1:



748620 . . . 748929,



DS985216.1:



748985 . . . 749555,



DS985216.1:



749607 . . . 749833,



DS985216.1:



749946 . . . 750572)


85081035
XM_951556.2:
56
XP_956649.1
365

Neurospora




215 . . . 2662




crassa








OR74A


145609083
XM_364271.2:
57
XP_364271.2
366

Magnaporthe




1 . . . 2442




grisea








70-15


171679277
XM_001904550.1:
58
XP_001904585.1
367

Podospora




1 . . . 2316




anserine



169859036
XM_001836107.1:
59
XP_001836159.1
368

Coprinopsis




1 . . . 2418




cinerea








okayama7#130


19112755
NM_001021872.1:
60
NP_595963.1
369

Schizosaccharomyces




1 . . . 2478




pombe



213405339
XM_002173405.1:
61
XP_002173441.1
370

Schizosaccharomyces




1 . . . 2469




japonicus








yFS27text missing or illegible when filed


58267408
XM_570860.1:
62
XP_570860.1
371

Cryptococcus




39 . . . 2594




neoformans








var.








neoformans








JEC21


71018661
XM_754468.1:
63
XP_759561.1
372

Ustilago




1 . . . 2682




maydis








52text missing or illegible when filed


254413307
NZ_DS989851.1:
64
ZP_05027078.1
373

Microcoleus




81897 . . . 84338




chthonoplastes








PCC 7420


256377454
NC_013093.1:
65
YP_003101114.1
374

Actinosynnema




3941285 . . . 3943633




mirum








DSM 43827


221195188
NZ_ACFE01000002.1:
66
ZP_03568244.1
375

Atopobium




241103 . . . 243577




rimae








ATCC 49626


257785020
complement
67
YP_003180237.1
376

Atopobium




(NC_013203.1:




parvulum




1365893 . . . 1368364)



DSM 20469


227516879
complement
68
ZP_03946928.1
377

Atopobium




(NZ_ACGK01000053.1:




vaginae




213016 . . . 215490)



DSM 15829


210630184
NZ_ABXJ01000012.1:
69
ZP_03296299.1
378

Collinsella




49466 . . . 51985




stercoris








DSM 13279


41056825
AY518215.1:
70
AAR98787.1
379

Bifidobacterium




989 . . . 3466




longum



223467373
NZ_ACCG01000015.1:
71
ZP_03618909.1
380

Bifidobacterium




9765 . . . 12350




breve








DSM 20213


224282874
NZ_ABQP01000009.1:
72
ZP_03646196.1
381

Bifidobacterium




218668 . . . 221073




bifidum








NCIMB 41171


229817819
complement
73
ZP_04448101.1
382

Bifidobacterium




(NZ_ABYS02000004.1:




angulatum




901411 . . . 903888)



DSM 20098


212716076
complement
74
ZP_03324204.1
383

Bifidobacterium




(NZ_ABXY01000011.1:




catenulatum




578312 . . . 580789)



DSM 16992


41056831
AY518218.1:
75
AAR98790.1
384

Bifidobacterium




1430 . . . 3907



sp. CFAR 172


41056829
AY518217.1:
76
AAR98789.1
385

Bifidobacterium




951 . . . 3428




pullorum



227507561
NZ_ACGF01000124.1:
77
ZP_03937610.1
386

Gardnerella




41655 . . . 44132




vaginalis








ATCC 14019


261337317
NZ_ABXB03000001.1:
78
ZP_05965201.1
387

Bifidobacterium




154886 . . . 157366




gallicum








DSM 20093


183601500
complement
79
ZP_02962870.1
388

Bifidobacterium




(NZ_ABOT01000001.1:




animalis




194894 . . . 197371)



subsp.








lactis








HN019


41056827
AY518216.1:
80
AAR98788.1
389

Bifidobacterium




988 . . . 3465




pseudolongum








subsp.








Globosum



227516469
complement
81
ZP_03946518.1
390

Atopobium




(NZ_ACGK01000047.1:




vaginae




28634 . . . 31102)



DSM 15829


76556241
AJ509177.1:
82
YP_001511171.1
391

Frankia




1 . . . 2625



sp. EAN1pec


158318663
NC_009921.1:
83
YP_713678.1
392

Frankia




8441355 . . . 8443790




alni








ACN14a


111222884
complement
84
YP_002778395.1
393

Rhodococcus




(NC_008278.1:




opacus




3758441 . . . 3760909)



B4


226360617
complement
85
YP_701466.1
394

Rhodococcus




(NC_012522.1:




jostii




1273076 . . . 1275478)



RHA1


111018494
complement
86
ZP_04383880.1
395

Rhodococcus




(NC_008268.1:




erythropolis




1575800 . . . 1578352)



SK121


229490027
NZ_ACNO01000014.1:
87
YP_947598.1
396

Arthrobacter




107516 . . . 109885




aurescens








TC1


119962524
NC_008711.1:
88
CAD48946.1
397

Propionibacterium




2018415 . . . 2020796




freudenreichii








subsp.








Shermanii



28868876
NC_004578.1:
89
NP_791495.1
398

Pseudomonas




1837381 . . . 1839888




syringae








pv. tomato







str. DC3000


256425339
NC_013132.1:
90
YP_003125992.1
399

Chitinophaga




8027760 . . . 8030123




pinensis








DSM 2588


161075783
EU223897.1:
91
ABX56639.1
400

Verrucomicrobiae




1 . . . 2430




bacterium








V4


218246512
complement
92
YP_002371883.1
401

Cyanothece




(NC_011726.1:



sp. PCtext missing or illegible when filed  8801



1758431 . . . 1760839)


172055269
NC_010547.1:
93
YP_001806596.1
402

Cyanothece




390265 . . . 392673



sp. ATCC 51142


126659520
complement
94
ZP_01730652.1
403

Cyanothece




(NZ_AAXW01000034.1:



sp. CCY0110



5415 . . . 7823)


258380665
complement
95
CAQ48286.1
404

Planktothrix




(AM990467.1:




rubescens




2704 . . . 5112)



NIVA-CYA98


209527806
NZ_ABYK01000067.1:
96
ZP_03276298.1
405

Arthrospira




8063 . . . 10480




maxima








CS-328


196258744
NZ_ABVE01000007.1:
97
ZP_03157277.1
406

Cyanothece




72906 . . . 75314



sp. PCtext missing or illegible when filed 7822


218440702
complement
98
YP_002379031.1
407

Cyanothece




(NC_011729.1:



sp. PCtext missing or illegible when filed 7424



4207741 . . . 4210149)


166366228
complement
99
YP_001658501.1
408

Microcystis




(NC_010296.1:




aeruginosa




3156762 . . . 3159182)



NIES-843


119488765
NZ_AAVU01000020.1:
100
ZP_01621774.1
409

Lyngbya




110903 . . . 113317



sp. PCC 8106


17228976
complement
101
NP_485524.1
410

Nostoc




(NC_003272.1:



sp. PCC 7120



1746056 . . . 1748482)


254422632
NZ_DS989904.1:
102
ZP_05036350.1
411

Synechococcus




4613864 . . . 4616290



sp. PCC 7335


158333641
NC_009925.1:
103
YP_001514813.1
412

Acaryochloris




422232 . . . 424652




marina








MBIC11017


254425820
complement
104
ZP_05039537.1
413

Synechococcus




(NZ_DS989905.1:



sp. PCC 7335



71540 . . . 74017)


170695087
complement
105
ZP_02886235.1
414

Burkholderia




(NZ_ABLD01000020.1:




graminis




33972 . . . 36368)



C4D1M


209515639
complement
106
ZP_03264503.1
415

Burkholderia




(NZ_ABYL01000006.1:



sp. H160



33232 . . . 35628)


87303015
NZ_AANO01000008.1:
107
ZP_01085819.1
416

Synechococcus




122233 . . . 124656



sp. WH 5701


254431900
complement
108
ZP_05045603.1
417

Cyanobium




(NZ_DS990556.1:



sp. PCtext missing or illegible when filed  7001



2146872 . . . 2149313)


88808134
NZ_AAOK01000002.1:
109
ZP_01123645.1
418

Synechococcus




342081 . . . 344516



sp. WH 7805


148238545
complement
110
YP_001223932.1
419

Synechococcus




(NC_009481.1:



sp. WH 7803



226771 . . . 229206)


87123187
NZ_AANP01000001.1:
111
ZP_01079038.1
420

Synechococcus




180603 . . . 183032



sp. RS9917


187919971
complement
112
YP_001889002.1
421

Burkholderia




(NC_010676.1:




phytofirmans




1450148 . . . 1452541)



PsJN


91778759
complement
113
YP_553967.1
422

Burkholderia




(NC_007952.1:




xenovorans




1882080 . . . 1884473)



LB400


170690542
NZ_ABLD01000001.1:
114
ZP_02881709.1
423

Burkholderia




565487 . . . 567880




graminis








C4D1M


209521856
NZ_ABYL01000194.1:
115
ZP_03270532.1
424

Burkholderia




6778 . . . 9171



sp. H160


186474278
complement
116
YP_001861620.1
425

Burkholderia




(NC_010623.1:




phymatum




2647064 . . . 2649448)



STM815


225873826
complement
117
YP_002755285.1
426

Acidobacterium




(NC_012483.1:




capsulatum




2598033 . . . 2600420)



ATCC 51196


206602403
DS995260.1:
118
EDZ38884.1
427

Leptospirillum




236338 . . . 238704



sp. Group II







′5-way CG


251772639
complement
119
EES53204.1
428

Leptospirillum




(GG693868.1:




ferrodiazotrophum




86578 . . . 88956)


56752022
complement
120
YP_172723.1
429

Synechococcus




(NC_006576.1:




elongatus




2156604 . . . 2158994)



PCC 630text missing or illegible when filed


22298729
complement
121
NP_681976.1
430

Thermosynechococcus




(NC_004113.1:




elongatus




1224195 . . . 1226633)



BP-1


53804073
NC_002977.6:
122
YP_114037.1
431

Methylococcus




1693459 . . . 1695894




capsulatus








str. Bath


220907266
NC_011884.1:
123
YP_002482577.1
432

Cyanothece




1725657 . . . 1728098



sp. PCtext missing or illegible when filed  7425


16332268
NC_000911.1:
124
NP_442996.1
433

Synechocystis




3500713 . . . 3503178



sp. PCC 6803


220907424
complement
125
YP_002482735.1
434

Cyanothece




(NC_011884.1:



sp. PCtext missing or illegible when filed  7425



1898702 . . . 1901167)


241777601
complement
126
ZP_04774866.1
435

Allochromatium




(NZ_ACQQ01000020.1:




vinosum




30393 . . . 32762)



DSM 180


114778289
NZ_AATS01000014.1:
127
ZP_01453148.1
436

Mariprofundus




23435 . . . 25801




ferrooxydans








PV-1


251827471
complement
128
ZP_04830548.1
437

Gallionella




(NZ_ACSD01000006.1:




ferruginea




39617 . . . 41986)



ES-2


121712503
XM_001273862.1:
129
XP_001273863.1
438

Aspergillus




1 . . . 2436




clavatu
text missing or illegible when filed








NRRL 1


119473535
XM_001258642.1:
130
XP_001258643.1
439

Neosartorya




1 . . . 2439




fischer
text missing or illegible when filed








NRRL 181


169763560
XM_001727628.1:
131
XP_001727680.1
440

Aspergillus




1 . . . 2433




oryzae








RIB40


145248115
XM_001396269.1:
132
XP_001396306.1
441

Aspergillus




1 . . . 2448




niger



115400974
XM_001216075.1:
133
XP_001216075.1
442

Aspergillus




1 . . . 2457




terreus








NIH2624


255952755
XM_002567084.1:
134
XP_002567130.1
443

Penicillium




1 . . . 2433




chrysogenum








Wisconsin 54-1255


212527388
XM_002143815.1:
135
XP_002143851.1
444

Penicillium




98 . . . 2551




marneffei








ATCC 18224


242783584
XM_002480171.1:
136
XP_002480216.1
445

Talaromyces




1 . . . 2448




stipitatus








ATCC 10500


154321267
XM_001559899.1:
137
XP_001559949.1
446

Botryotinia




1 . . . 2466




fuckeliana








B05.10


156054348
XM_001593050.1:
138
XP_001593100.1
447

Sclerotinia




1 . . . 2499




sclerotiorum








1980


189191706
XM_001932157.1:
139
XP_001932192.1
448

Pyrenophora




1 . . . 2469




tritici-repentis








Pt-1C-BFP


169600613
XM_001793677.1:
140
XP_001793729.1
449

Phaeosphaeria




1 . . . 2466




nodorum








SN15


58260732
XM_567776.1:
141
XP_567776.1
450

Cryptococcus




41 . . . 2545




neoformans








var.








neoformans








JEC21


46123901
XM_386504.1:
142
XP_386504.1
451

Gibberella




1 . . . 2460




zeae








PH-1


256732917
complement
143
EEU46265.1
452

Nectria




(join(GG698898.1:




haematococca




321524 . . . 322233,



mpV 77-13-4



GG698898.1:



322285 . . . 322489,



GG698898.1:



322540 . . . 324081))


225729111
FJ790496.1:
144
ACO24516.1
453

Metarhizium




215 . . . 2677




anisopliae



85094948
XM_954892.2:
145
XP_959985.1
454

Neurospora




155 . . . 2638




crassa








OR74A


171679479
XM_001904651.1:
146
XP_001904686.1
455

Podospora




1 . . . 2517




anserine



198283820
NC_011206.1:
147
YP_002220141.1
456

Acidithiobacillus




1682860 . . . 1685307




ferrooxidans








ATCC 53993


148243889
NC_009468.1:
148
YP_001220128.1
457

Acidiphilium




90683 . . . 93145




cryptum








JF-5


157364435
NC_009828.1:
149
YP_001471202.1
458

Thermotoga




1658895 . . . 1661258




lettingae








TMO


217966781
NC_011661.1:
150
YP_002352287.1
459

Dictyoglomus




369050 . . . 371428




turgidum








DSM 672text missing or illegible when filed


92109503
complement
151
YP_571790.1
460

Nitrobacter




(NC_007960.1:




hamburgensis




14429 . . . 16810)



X14


87310270
complement
152
ZP_01092401.1
461

Blastopirellula




(NZ_AANZ01000017.1:




marina




80191 . . . 82560)



DSM 3645


152995974
NC_009654.1:
153
YP_001340809.1
462

Marinomonas




2214232 . . . 2216625



sp. MWYL1


32473390
NC_005027.1:
154
NP_866384.1
463

Rhodopirellula




2520925 . . . 2523306




baltica








SH 1


254495580
complement
155
ZP_05108502.1
464

Legionella




(NZ_ACUL01000002.1:




drancourtii




21176 . . . 23557)



LLAP12


254380451
NZ_DS570384.1:
156
ZP_04995817.1
465

Streptomyces




88623 . . . 90992



sp. Mg1


227974767
NZ_ACGW01000133.1:
157
ZP_04023055.1
466

Lactobacillus




1172 . . . 3235




reuter








SD2112


227530011
NZ_ACGV01000134.1:
158
ZP_03960060.1
467

Lactobacillus




2320 . . . 4794




vaginalis








ATCC 49540


194467185
complement
159
ZP_03073172.1
468

Lactobacillus




(NZ_AAPZ02000001.1:




reuter




905298 . . . 907709)



100-23


256847586
NZ_GG698803.1:
160
ZP_05553031.1
469

Lactobacillus




21616 . . . 24015




coleohominis








101-4 CHN


260662452
complement
161
ZP_05863347.1
470

Lactobacillus




(NZ_GG704700.1:




fermentum




145244 . . . 147643)



28-3-CHN


227903484
NZ_ACHN01000046.1:
162
ZP_04021289.1
471

Lactobacillus




59035 . . . 61452




acidophilus








ATCC 4796


227877116
NZ_ACKR01000020.1:
163
ZP_03995194.1
472

Lactobacillus




11753 . . . 14191




crispatus








JV-V01


227893117
NZ_ACGU01000035.1:
164
ZP_04010922.1
473

Lactobacillus




36787 . . . 39186




ultunensis








DSM 16047


256844475
NZ_GG698762.1:
165
ZP_05549961.1
474

Lactobacillus




280846 . . . 283242




crispatus








125-2-CHN


227521312
complement
166
ZP_03951361.1
475

Lactobacillus




(NZ_ACGO01000008.1:




gasseri




37191 . . . 39647)



JV-V03


259501613
complement
167
ZP_05744515.1
476

Lactobacillus




(NZ_ACLN01000019.1:




iners




10173 . . . 12569)



DSM 13335


104773655
NC_008054.1:
168
YP_618635.1
477

Lactobacillus




449229 . . . 451631




delbrueckii








subsp.








bulgaricus








ATCC 11842


227525868
NZ_ACGQ01000037.1:
169
ZP_03955917.1
478

Lactobacillus




36941 . . . 39310




jensenii








JV-V16


227512366
NZ_ACGH01000107.1:
170
ZP_03942415.1
479

Lactobacillus




31655 . . . 34045




buchneri








ATCC 11577


118587374
complement
171
ZP_01544800.1
480

Oenococcus




(NZ_AAUV01000059.1:




oeni




59008 . . . 61416)



ATCC BAA-1163


28379168
complement
172
NP_786060.1
481

Lactobacillus




(NC_004567.1:




plantarum




2362936 . . . 2365302)



WCFS1


21363093
AJ309011.1:
173
Q937F6
482
XPKA_LACPE



181 . . . 2547


81427904
NC_007576.1:
174
YP_394903.1
483

Lactobacillus




286496 . . . 288859




sakei subsp.









sakei








23K


116492156
NC_008525.1:
175
YP_803891.1
484

Pediococcus




398927 . . . 401290




pentosaceus








ATCC 25745


259648565
AP011548.1:
176
BAI40727.1
485

Lactobacillus




211570 . . . 213957




rhamnosus








GG


227510093
complement
177
ZP_03940142.1
486

Lactobacillus




(NZ_ACGG01000115.1:




brevis




64541 . . . 66952)



subsp.








gravesensis








ATCC 27305


227891468
complement
178
ZP_04009273.1
487

Lactobacillus




(NZ_ACGT01000007.1:




salivarius




44265 . . . 46625)



ATCC 11741


227528594
NZ_ACGS01000122.1:
179
ZP_03958643.1
488

Lactobacillus




352 . . . 2721




ruminis








ATCC 25644


229542373
complement
180
ZP_04431433.1
489

Bacillus




(NZ_AAWV02000001.1:




coagulans




1384102 . . . 1386486)



36D1


238021480
complement
181
ZP_04601906.1
490

Kingella




(NZ_ACJW02000002.1:




oralis




913355 . . . 915730)



ATCC 51147


259046526
NZ_ACKZ01000008.1:
182
ZP_05736927.1
491

Granulicatella




36586 . . . 38955




adiacens








ATCC 49175


157150221
NC_009785.1:
183
YP_001449631.1
492

Streptococcus




333239 . . . 335623




gordonii








str.








Challis








substr. CH1


25011879
complement
184
NP_736274.1
493

Streptococcus




(NC_004368.1:




agalactiae




1900754 . . . 1903132)



NEM316


229555065
complement
185
ZP_04442854.1
494

Listeria




(NZ_ACCR01000006.1:




grayi




74043 . . . 76418)



DSM 20601


257866707
NZ_GG670386.1:
186
ZP_05646360.1
495

Enterococcus




478278 . . . 480644




casseliflavus








EC30


257870669
NZ_GG670289.1:
187
ZP_05650322.1
496

Enterococcus




233512 . . . 235875




gallinarum








EG2


257895654
NZ_GG670306.1:
188
ZP_05675307.1
497

Enterococcus




612981 . . . 615353




faecium








Com12


238810139
AP009608.1:
189
BAH69929.1
498

Mycoplasma




744956 . . . 747334




fermentans








PG18


193216764
NC_011025.1:
190
YP_002000006.1
499

Mycoplasma




384420 . . . 386801




arthritidis








158L3-1


148377390
NC_009497.1:
191
YP_001256266.1
500

Mycoplasma




136795 . . . 139182




agalactiae








PG2


191639669
NC_010999.1:
192
YP_001988835.1
501

Lactobacillus




2885324 . . . 2887711




casei








BL23


28379861
NC_004567.1:
193
NP_786753.1
502

Lactobacillus




3169067 . . . 3171478




plantarum








WCFS1


227892171
complement
194
ZP_04009976.1
503

Lactobacillus




(NZ_ACGT01000037.1:




salivarius




21330 . . . 23759)



ATCC 11741


116618551
NC_008531.1:
195
YP_818922.1
504

Leuconostoc




1449343 . . . 1451709




mesenteroides








subsp.








mesenteroides








ATCC 8293


116333142
NC_008497.1:
196
YP_794669.1
505

Lactobacillus




507704 . . . 510163




brevis








ATCC 367


241895257
complement
197
ZP_04782553.1
506

Weissella




(NZ_ACKU01000007.1:




paramesenteroides




101374 . . . 103833)



ATCC 33313


170016535
NC_010471.1:
198
YP_001727454.1
507

Leuconostoc




181964 . . . 184417




citreum








KM20


116619034
complement
199
YP_819405.1
508

Leuconostoc




(NC_008531.1:




mesenteroides




1934181 . . . 1936622)



subsp.








mesenteroides








ATCC 8293


161702316
EU255918.1:
200
ABX75772.1
509

Lactococcus




18411 . . . 20879




lactis








subsp.








Lactis



116491770
complement
201
YP_811314.1
510

Oenococcus




(NC_008528.1:




oeni




1731509 . . . 1733962)



PSU-1


182419955
complement
202
ZP_02951191.1
511

Clostridium




(NZ_ABDT01000107.2:




butyricum




13616 . . . 15991)



5521


255523324
complement
203
ZP_05390294.1
512

Clostridium




(NZ_ACVI01000003.1:




carboxidivorans




55354 . . . 57747)



P7


15894622
NC_003030.1:
204
NP_347971.1
513

Clostridium




1482782 . . . 1485172




acetobutylicum








ATCC 824


226324778
complement
205
ZP_03800296.1
514

Coprococcus




(NZ_ABVR01000041.1:




comes




500857 . . . 503232)



ATCC 27758


253580358
NZ_GG696051.1:
206
ZP_04857624.1
515

Ruminococcus




158015 . . . 160390



sp. 5_1_39B_FAA


257413435
NZ_ABYJ02000055.1:
207
ZP_04743029.2
516

Roseburia




10320 . . . 12779




intestinalis








L1-82


154500233
complement
208
ZP_02038271.1
517

Bacteroides




(NZ_AAXG02000041.1:




capillosus




34174 . . . 36609)



ATCC 29799


219119570
XM_002180506.1:
209
XP_002180542.1
518

Phaeodactylum




1 . . . 2508




tricornutum








CCAP 1055/1


91975971
NC_007958.1:
210
YP_568630.1
519

Rhodopseudomonas




1660408 . . . 1662762




palustris








BisB5


86750966
complement
211
YP_487462.1
520

Rhodopseudomonas




(NC_007778.1:




palustris




4411322 . . . 4413676)



HaA2


39934743
NC_005296.1:
212
NP_947019.1
521

Rhodopseudomonas




1858439 . . . 1860790




palustris








CGA009


90425290
complement
213
YP_533660.1
522

Rhodopseudomonas




(NC_007925.1:




palustris




4235875 . . . 4238229)



BisB18


121583071
NC_008758.1:
214
YP_973512.1
523

Polaromonas




65532 . . . 67904




naphthalenivorans








CJ2


115376972
complement
215
ZP_01464191.1
524

Stigmatella




(NZ_AAMD01000095.1:




aurantiaca




4173 . . . 6533)



DW4/3-1


148547676
complement
216
YP_001267778.1
525

Pseudomonas




(NC_009512.1:




putid
text missing or illegible when filed




2807645 . . . 2810020)



F1


116668711
NC_008541.1:
217
YP_829644.1
526

Arthrobacter




145493 . . . 147928



sp. FB24


220911083
NC_011886.1:
218
YP_002486392.1
527

Arthrobacter




321712 . . . 324174




chlorophenolicus








A6


260517200
complement
219
ZP_05816651.1
528

Sanguibacter




(NZ_ABUN01000002.1:




keddieii




92936 . . . 95461)



DSM 10542


229821527
NC_012669.1:
220
YP_002883053.1
529

Beutenbergia




3398743 . . . 3401217




cavernae








DSM 12333


256832813
NC_013174.1:
221
YP_003161540.1
530

Jonesia




1712156 . . . 1714588




denitrifican
text missing or illegible when filed








DSM 20603


227428425
complement
222
ZP_03911482.1
531

Xylanimonas




(NZ_ABVC01000008.1:




cellulosilytica




152502 . . . 154988)



DSM 15894


165929357
AM182260.1:
223
CAJ57850.1
532

Cellulomonas




1 . . . 2481




flavigena



145223927
NC_009338.1:
224
YP_001134605.1
533

Mycobacterium




3525804 . . . 3528275




gilvum








PYR-GCK


120404048
NC_008726.1:
225
YP_953877.1
534

Mycobacterium




3236585 . . . 3239083




vanbaalenii








PYR-1


257069356
NC_013172.1:
226
YP_003155611.1
535

Brachybacterium




2493744 . . . 2496215




faecium








DSM 4810


256824167
NC_013169.1:
227
YP_003148127.1
536

Kytococcus




273585 . . . 276047




sedentarius








DSM 20547


148271607
NC_009480.1:
228
YP_001221168.1
537

Clavibacter




506602 . . . 509040




michiganensis








subsp.








michiganensis








NCPPB 382


145594129
complement
229
YP_001158426.1
538

Salinispora




(NC_009380.1:




tropica




1798516 . . . 1800918)



CNB-440


159037167
complement
230
YP_001536420.1
539

Salinispora




(NC_009953.1:




arenicola




1767167 . . . 1769569)



CNS-205


238063593
complement
231
ZP_04608302.1
540

Micromonospora




(NZ_GG657738.1:



sp ATCC 39149



5405062 . . . 5407251)


118469963
NC_008596.1:
232
YP_887914.1
541

Mycobacterium




3674267 . . . 3676639




smegmatis








str. MC2 155


108799759
NC_008146.1:
233
YP_639956.1
542

Mycobacterium




2939527 . . . 2941947



sp. MCS


240170498
complement
234
ZP_04749157.1
543

Mycobacterium




(NZ_ACBV01000039.1:




kansasii




21 . . . 2423)



ATCC 12478


183982748
complement
235
YP_001851039.1
544

Mycobacterium




(NC_010612.1:




marinum




3341817 . . . 3344219)



M


41407671
complement
236
NP_960507.1
545

Mycobacterium




(NC_002944.2:




avium




1726717 . . . 1729131)



subsp.








paratuberculosis








K-10


254819329
NZ_ABIN01000047.1:
237
ZP_05224330.1
546

Mycobacterium




36474 . . . 38837




intracellulare








ATCC 13950


169629591
complement
238
YP_001703240.1
547

Mycobacterium




(NC_010397.1:




abscessus




2559451 . . . 2561871)


84496279
complement
239
ZP_00995133.1
548

Janibacter




(NZ_AAMN01000002.1:



sp. HTCC2649



433314 . . . 435707)


72163369
NC_007333.1:
240
YP_291026.1
549

Thermobifida




3478272 . . . 3480650




fusca








YX


227984600
complement
241
ZP_04031845.1
550

Thermomonospora




(NZ_ABUZ01000013.1:




curvata




70531 . . . 72909)



DSM 43183


229855558
complement
242
ZP_04475514.1
551

Streptosporangium




(NZ_ABUU01000106.1:




roseum




1385 . . . 3700)



DSM 43021


229209207
NZ_ABUI01000028.1:
243
ZP_04335641.1
552

Nocardiopsis




55841 . . . 58189




dassonvillei








subsp.








dassonvillei








DSM 43111


229862587
NZ_ABUS01000001.1:
244
ZP_04482201.1
553

Stackebrandtia




1911934 . . . 1914330




nassauensis








DSM 44728


256376052
NC_013093.1:
245
YP_003099712.1
554

Actinosynnema




2125566 . . . 2127935




mirum








DSM 43827


32141117
complement
246
NP_733508.1
555

Streptomyces




(NC_003888.3:




coelicolor




656319 . . . 658772)



A3(2)


117164830
complement
247
CAJ88379.1
556

Streptomyces




(AM238664.1:




ambofaciens




846551 . . . 849055)



ATCC 23877


256811868
complement
248
ZP_05536883.1
557

Streptomyces




(NZ_ACFA01000015.1:




griseoflavus




3377 . . . 5761)



Tu400text missing or illegible when filed


254405496
complement
249
ZP_05020421.1
558

Streptomyces




(NZ_DS570938.1:




sviceus




43288 . . . 45726)



ATCC 29083


260644540
complement
250
CBG67625.1
559

Streptomyces




(FN554889.1:




scabiei




480316 . . . 482694)



87.22


29827814
complement
251
NP_822448.1
560

Streptomyces




(NC_003155.4:




avermitilis




1579336 . . . 1581717)



MA-4680


239932594
NZ_ABYA01000503.1:
252
ZP_04689547.1
561

Streptomyces




5217 . . . 7595




ghanaensis








ATCC 14672


256800397
complement
253
ZP_05530021.1
562

Streptomyces




(NZ_ACEZ01000048.1:




viridochromogenes




24916 . . . 27303)



DSM 40736


256774038
complement
254
ZP_05512501.1
563

Streptomyces




(NZ_ACEX01000074.1:




hygroscopicus




46221 . . . 48614)



ATCC 53653


260452518
NZ_ACZH01000001.1:
255
ZP_05800927.1
564

Streptomyces




321972 . . . 324359




flavogriseus








ATCC 33331


182440556
NC_010572.1:
256
YP_001828275.1
565

Streptomyces




8084439 . . . 8086826




griseus








subsp.








griseus








NBRC 13350


239982969
NZ_ABYC01000425.1:
257
ZP_04705493.1
566

Streptomyces




13265 . . . 15646




albus








J1074


254381599
NZ_DS570386.1:
258
ZP_04996963.1
567

Streptomyces




118817 . . . 121204



sp. Mg1


256674998
NZ_ACEU01000020.1:
259
ZP_05485309.1
568

Streptomyces




1507 . . . 3900



sp. SPB78


227377421
NZ_ABUC01000002.1:
260
ZP_03860882.1
569

Kribbella




229225 . . . 231603




flavida








DSM 17836


54023297
complement
261
YP_117539.1
570

Nocardia




(NC_006361.1:




farcinica




1487629 . . . 1490097)



IFM 10152


158313048
NC_009921.1:
262
YP_001505556.1
571

Frankia




1426213 . . . 1428621



sp. EAN1pec


86742227
complement
263
YP_482627.1
572

Frankia




(NC_007777.1:



sp. CcI3



4238578 . . . 4240986)


256395329
NC_013131.1:
264
YP_003116893.1
573

Catenulispora




7133131 . . . 7135533




acidiphila








DSM 44928


117927729
NC_008578.1:
265
YP_872280.1
574

Acidothermus




555555 . . . 557948




cellulolyticus








11B


119717842
complement
266
YP_924807.1
575

Nocardioides




(NC_008699.1:



sp. JS614



3839565 . . . 3841961)


134098496
NC_009142.1:
1267
YP_001104157.1
576

Saccharopolyspora




2098116 . . . 2100512




erythraea








NRRL 2338


209550756
NC_011369.1:
268
YP_002282673.1
577

Rhizobium




3264963 . . . 3267347




leguminosarum








bv.








trifolii








WSM2304


241206160
NC_012850.1:
269
YPJ002977256.1
578

Rhizobium




3503904 . . . 3506288




leguminosarum








bv.








trifolii








WSM1325


190893254
NC_010994.1:
270
YP_001979796.1
579

Rhizobium




3714233 . . . 3716620




etli








CIAT 652


86359034
NC_007761.1:
271
YP_470926.1
580

Rhizobium




3623921 . . . 3626308




etli








CFtext missing or illegible when filed 42


222081270
complement
272
YP_002540633.1
581

Agrobacterium




(NC_011983.1:




radiobacter




490969 . . . 493383)



K84


254720555
NZ_ACBQ01000064.1:
273
ZP_05182366.1
582

Brucella




129340 . . . 131718



sp. 83/13


239835057
complement
274
ZP_04683384.1
583

Ochrobactrum




(NZ_ACQA01000003.1:




intermedium




10528 . . . 13017)



LMG 3301


153012043
NC_009671.1:
275
YP_001373254.1
584

Ochrobactrum




16319 . . . 18706




anthropi








ATCC 49188


146339061
complement
276
YP_001204109.1
585

Bradyrhizobium




(NC_009445.1:



sp. ORS278



2141749 . . . 2144226)


148253833
complement
277
YP_001238418.1
586

Bradyrhizobium




(NC_009485.1:



sp. BTAi1



2424642 . . . 2427059)


27377629
complement
278
NP_769158.1
587

Bradyrhizobium




(NC_004463.1:




japonicum




2749734 . . . 2752139)



USDA 110


92117435
complement
279
YP_577164.1
588

Nitrobacter




(NC_007964.1:




hamburgensis




2109162 . . . 2111570)



X14


240137143
NC_012808.1:
280
YP_002961612.1
589

Methylobacterium




407982 . . . 410417




extorquens








AM1


110634584
complement
281
YP_674792.1
590

Mesorhizobium




(NC_008254.1:



sp. BNC1



2388345 . . . 2390747)


260467447
NZ_ACZA01000051.1:
282
ZP_05813617.1
591

Mesorhizobium




15952 . . . 18360




opportunistum








WSM2075


75676138
NC_007406.1:
283
YP_318559.1
592

Nitrobacter




2135469 . . . 2137856




winogradskyi








Nb-255


170749020
complement
284
YP_001755280.1
593

Methylobacterium




(NC_010505.1:




radiotolerans




2769888 . . . 2772470)



JCM 2831


170746859
complement
285
YP_001753119.1
594

Methylobacterium




(NC_010505.1:




radiotolerans




465997 . . . 468552)



JCM 2831


254558916
NC_012988.1:
286
YP_003066011.1
595

Methylobacterium




271224 . . . 273809




extorquens








DM4


240140298
NC_012808.1:
287
YP_002964777.1
596

Methylobacterium




3931130 . . . 3933676




extorquens








AM1


220925990
NC_011894.1:
288
YP_002501292.1
597

Methylobacterium




6291823 . . . 6294321




nodulans








ORS 2060


220919962
complement
289
YP_002495265.1
598

Methylobacterium




(NC_011892.1:




nodulans




451339 . . . 453840)



ORS 2060


170741732
NC_010511.1:
290
YP_001770387.1
599

Methylobacterium




3988668 . . . 3991166



sp. 4-46


239815802
complement
291
YP_002944712.1
600

Variovorax




(NC_012791.1:




paradoxus




2971257 . . . 2973608)



S110


89069402
NZ_AAOT01000017.1:
292
ZP_01156757.1
601

Oceanicola




31124 . . . 33460




granulosus








HTCC2516


119509641
complement
293
ZP_01628787.1
602

Nodularia




(NZ_AAVW01000007.1:




spumigena




15878 . . . 18259)



CCY9414


186682350
NC_010628.1:
294
YP_001865546.1
603

Nostoc




2389837 . . . 2392218




punctiforme








PCC 73102


75906719
complement
295
YP_321015.1
604

Anabaena




(NC_007413.1:




variabilis




617971 . . . 620352)



ATCC 29413


225522346
NZ_ACIR01000182.1:
296
ZP_03769140.1
605

Nostoc




624 . . . 2756




azollae








070text missing or illegible when filed


37520566
NC_005125.1:
297
NP_923943.1
606

Gloeobacter




1065716 . . . 1068097




violaceus








PCC 7421


86608623
NC_007776.1:
298
YP_477385.1
607

Synechococcus




1182311 . . . 1184686



sp. JA-2-







3B′a(2-13)


150398192
complement
299
YP_001328659.1
608

Sinorhizobium




(NC_009636.1:




medicae WSM419




3144485 . . . 3146857)


116249832
complement
300
YP_765670.1
609

Rhizobium




(NC_008380.1:




leguminosarum




82152 . . . 84617)



bv.








viciae 3841



195970218
complement
301
NP_384212.2
610

Sinorhizobium




(NC_003047.1:




meliloti




123688 . . . 126141)



1021


171912985
NZ_ABIZ01000001.1:
302
ZP_02928455.1
611

Verrucomicrobium




4370841 . . . 4373354




spinosum








DSM 4136


163849496
complement
303
YP_001637539.1
612

Methylobacterium




(NC_010172.1:




extorquens




46285 . . . 48720)



PA1


85714839
NZ_AAMY01000005.1:
304
ZP_01045825.1
613

Nitrobacter




72844 . . . 75210



sp. Nb-311A


168704325
complement
305
ZP_02736602.1
614

Gemmata




(NZ_ABGO01000323.1:




obscuriglobus




57 . . . 2462)



UQtext missing or illegible when filed  2246


256829143
complement
306
YP_003157871.1
615

Desulfomicrobium




(NC_013173.1:




baculatum




1530488 . . . 1532881)



DSM 4028


223939426
complement
307
ZP_03631304.1
616
bacterium



(NZ_ABOX02000044.1:



Ellin514



41392 . . . 43863)


237747078
complement
308
ZP_04577558.1
617

Oxalobacter




(NZ_GG658151.1:




formigenes




2005797 . . . 2008190)



HOxBLS


237749232
complement
309
ZP_04579712.1
618

Oxalobacter




(NZ_GG658170.1:




formigenes




2042015 . . . 2044411)



OXCC13


116624013
NC_008536.1:
310
YP_826169.1
619

Solibacter




6218168 . . . 6220537




usitatus








Ellin6076


194336959
complement
311
YP_002018753.1
620

Pelodictyon




(NC_011060.1:




phaeoclathratiforme




2004498 . . . 2006885)



BU-1


194334425
complement
312
YP_002016285.1
621

Prosthecochloris




(NC_011059.1:




aestuarii




1762093 . . . 1764489)



DSM 271


189346840
complement
313
YP_001943369.1
622

Chlorobium




(NC_010803.1:




limicola




1427679 . . . 1430054)



DSM 245


21674344
complement
314
NP_662409.1
623

Chlorobium




(NC_002932.3:




tepidum




1423776 . . . 1426289)



TLS


110597897
complement
315
ZP_01386179.1
624

Chlorobium




(NZ_AASE01000009.1:




ferrooxidans




37756 . . . 40179)



DSM 13031


78187379
complement
316
YP_375422.1
625

Chlorobium




(NC_007512.1:




luteolum




1709621 . . . 1712050)



DSM 273


71907690
complement
317
YP_285277.1
626

Dechloromonas




(NC_007298.1:




aromatica




2220090 . . . 2222456)



RCB


74316849
NC_007404.1:
318
YP_314589.1
627

Thiobacillus




876540 . . . 878978




denitrificans








ATCC 25259


91775246
complement
319
YP_545002.1
628

Methylobacillus




(NC_007947.1:




flagellatus




935825 . . . 938200)



KT


30250069
NC_004757.1:
320
NP_842139.1
629

Nitrosomonas




2318109 . . . 2320481




europaea








ATCC 19718


114332052
NC_008344.1:
321
YP_748274.1
630

Nitrosomonas




2209596 . . . 2211971




eutrqpha








C91


82702122
NC_007614.1:
322
YP_411688.1
631

Nitrosospira




1152112 . . . 1154535




multiformis








ATCC 25196


77166175
NC_007484.1:
323
YP_344700.1
632

Nitrosococcus




3082455 . . . 3084869




oceani








ATCC 1970text missing or illegible when filed


46445639
complement
324
YP_007004.1
633

Candidatus




(NC_005861.1:




Protochlamydia




5907 . . . 8303)




amoebophila








UWE25


16263040
complement
325
NP_435833.1
634

Sinorhizobium




(NC_003037.1:




meliloti




591065 . . . 593440)



1021


229532493
NZ_ABUV01000006.1:
326
ZP_04421874.1
635

Sulfurospirillum




47234 . . . 49585




deleyianum








DSM 6946


13475490
NC_002678.2:
327
NP_107054.1
636

Mesorhizobium




5384229 . . . 5386652




loti








MAFF303099


209885940
complement
328
YP_002289797.1
637

Oligotropha




(NC_011386.1:




carboxidovorans




2786353 . . . 2788746)



OM5


182679166
NC_010581.1:
329
YP_001833312.1
638

Beijerinckia




2524033 . . . 2526420




indica








subsp.








indica








ATCC 9039






text missing or illegible when filed indicates data missing or illegible when filed







Numerous examples of polynucleotides, genes and/or polypeptides encoding phosphotransacetylase are known in the art and can be used in relation to the recombinant host cells disclosed herein. In embodiments, the phosphotransacetylase can be EutD from Lactobacillus plantarum. In embodiments, the phosphotransacetylase can be the phosphotransacetylase from Bacillus subtilis. This phosphotransacetylase has a specific activity of 1371 mmol/min/mg and a Km 0.06 mM for acetyl-CoA (Rado and Hoch, Biochim. Biophys. Acta. 321: 114-25; 1973). In addition, the equilibrium constant (Keq) of this reaction was found to be 154±14 in favor of the formation of acetyl-CoA according to the following formula:









[

acetyl


-


CoA

]



[
Pi
]




[
CoA
]



[

acetyl


-


P

]



=
Keq




In embodiments, host cells comprise a polypeptide having at least about 80%, at least about 85%, at least about 90%, or at least about 99% identity to a polypeptide of Table 10 or an active fragment thereof or a polynucleotide encoding such a polypeptide. In embodiments, the phosplotransacetylase can be a polypeptide having at least about 80%, at least about 85%, at least about 90%, at least about 95%, or at least about 99% identity to SEQ ID NO: 1472 or an active fragment thereof. In other embodiments, a polynucleotide, gene and/or polypeptide encoding phosphotransacetylase can include, but is not limited to, a sequence provided in the following tables 10 or 12.









TABLE 10







SEQ ID NOs of phosphotransacetylase target gene coding regions and proteins.











SEQ ID
SEQ ID




NO:
NO:




Nucleic
Amino



Description
acid
acid
Amino acid sequence





EutD
1111
1472
MDLFESLAQKITGKDQTIVFPEGTEPRIVGAAARLAADGLVKPIVLGATDKVQAVAND text missing or illegible when filed


phospho-


ADLTGVQVLDPATYPAEDKQAMLDALVERRKGKNTPEQAAKMLEDENYFGTMLVYMGKA


trans-


GMVSGAIHPTGDTVRPALQIIKTKPGSHRISGAFIMQKGEERYVFADCAINIDPDADT text missing or illegible when filed


acetylase


EIATQSAATAKVFDIDPKVAMLSFSTKGSAKGEMVTKVQEATAKAQAAEPELAIDGEL text missing or illegible when filed


from Lacto-



DAAFVEKVGLQKAPGSKVAGHANVFVFPELQSGNIGYKIAQRFGHFEAVGPVLQGLNK text missing or illegible when filed




bacillus




SDLSRGCSEEDVYKVAIITAAQGLA




plantarum









Phospho-
1061
1422
MADLFSTVQEKVAGKDVKIVFPEGLDERILEAVSKLAGNKVLNPIVIGNENEIQAKAK text missing or illegible when filed


trans-


NLTLGGVKIYDPHTYEGMEDLVQAFVERRKGKATEEQARKALLDENYFGTMLVYKGLA text missing or illegible when filed


acetylase



LVSGAAHSTADTVRPALQIIKTKEGVKKTSGVFIMARGEEQYVFADCAINIAPDSQDL text missing or illegible when filed



from


IAIESANTAKMFDIEPRVAMLSFSTKGSAKSDETEKVADAVKIAKEKAPELTLDGEFQ text missing or illegible when filed



Bacillus



AAFVPSVAEKKAPDSEIKGDANVFVFPSLEAGNIGYKIAQRLGNFEAVGPILQGLNMP text missing or illegible when filed



subtilis




DLSRGCNAEDVYNLALITAAQAL







text missing or illegible when filed indicates data missing or illegible when filed







Additional suitable phosphotransacetylase target gene coding regions and proteins were identified by diversity searching and clustering. A blast search of the non redundant GenBank protein database (NR) was performed with the L. plantarum EutD protein as a query sequence. A blast cut-off (Evalue) of 0.01 was applied. This search resulted in 2124 sequence matches. Redundancy reduction was achieved by clustering proteins with the CD-HIT program with parameters set at 95% sequence identity and 90% length overlap. The longest seed sequence, representative of each cluster, is retained for further analysis. Clustering reduced the number of protein sequences to 1336. Further clean-up of the sequences by removing sequences <280 amino acids and sequences >795 amino acids resulted in 1231 seqs.


The Brenda database was queried for experimentally verified phosphate acetyltransferases. Thirteen were found in the following organisms: S. enterica, E. coli K12, V. Parvula, C. Kluyveri, C. Acetobutylicum, C. Thermocellum, M thermophila, S. pyogenes, B. subtilis, L. fermentum, L. plantarum, L. sanfranciscensis, B subtilis, L. fermentum, L. plantarum, L. sanfranciscensis, R. palustris, E. coli.


Experimentally verified phosphate acetyltransferases (EC 2.3.1.8) belong to the PTA_PTB pfam family. However, the PTA_PTB domain is present in 13 distinct architectures (http://pfam.janelia.org/family/PF01515, Pfam database version 24). The motivation for investigating the domain architecture is to determine which of the proteins, that were identified by BLAST search, are likely to be phosphate acetyltransferases.


Experimentally verified sequences extracted from the BRENDA database as well as sequences retained after the CD-HIT clustering and clean-up, were searched against the Pfam database to determine their domain architecture. Pfam is a collection of multiple sequence alignments and profile hidden Markov models (HMMs). Each Pfam HMM represents a protein family or domain. By searching a protein sequence against the Pfam library of HMMs, it is possible to determine which domains it carries i.e. its domain architecture. (The Pfam protein families database: R. D. Finn, J. Tate, J. Mistry, P. C. Coggill, J. S. Sammut, H. R. Hotz, G. Ceric, K. Forslund, S. R. Eddy, E. L. Sonnhammer and A. Bateman Nucleic Acids Research (2008) Database Issue 36:D281-D288)


Twelve of the experimentally verified proteins only contained the PTA_PTB domain. Two sequences, from R. palustris and E. coli, contained two domains PTA_PTB and DRTGG, a domain of unknown function. Therefore, from the CD-HIT clustering results, proteins that contained either the PTA_PTB domain only (Group 1: 549 sequences) or a combination of PTA_PTB+DRTGG domains (Group 2: 201 sequences) were chosen.


Furthermore, the PTA_PTB domain, as the name indicates, is actually not specific to phosphate acetyltransferases. The domain is also a signature for phosphate butyryltransferases (EC 2.3.1.19) methods to distinguish between the two subfamilies: acetyltransferases and butyryltransferases were employed and are as follows:


To further characterize the relationship among the sequences, multiple sequence alignment MSA), phylogenetic analysis. profile HMMs and GroupSim analysis were performed, For this set of analyses, the phosphate acetyltransferases are split in two groups. Group 1 contains phosphate acetyltransferases with the PTA_PTB domain only, while Group 2 contains phosphate acetyltransferases with PTA_PTB+DRTGG. The motivation here is to generate groups with similar lengths.


Clustal X, version 2.0 was used for sequence alignments with default parameters. (Thompson J D, et al. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. (1997) 25:4876-4882.)


Alignment results were utilized to compute % sequence identities to a reference sequence. If the sequence from L. plantarum is taken as a reference, % IDs range from as low as 10.5% to 75.6% for the closest sequence. Alignment results also provided the basis for re-constructing phylogenetic trees. The Neighbor Joining method, available in the Jalview package version 2.3, was used to produce the trees, and computed trees were visualized in MEGA 4 (Tamura, Dudley, Nei, and Kumar 2007). The Neighbor Joining method is a method for re-constructing phylogenetic trees, and computing the lengths of the branches of this tree. In each stage, the two nearest nodes of the tree (the term “nearest nodes” will be defined in the following paragraphs) are chosen and defined as neighbors in our tree. This is done recursively until all of the nodes are paired together. “The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 July; 4(4):406-25. Saitou N, Nei M.” Jalview Version 2 is a system for interactive editing, analysis and annotation of multiple sequence alignments (Waterhouse, A. M., Procter, J. B., Martin, D. M. A, Clamp, M. and Barton, G. J. (2009) “Jalview Version 2—a multiple sequence alignment editor and analysis workbench” Bioinformatics 25 (9) 1189-1191). The MEGA software provides tools for exploring, discovering, and analyzing DNA and protein sequences from an evolutionary perspective. MEGA4 enables the integration of sequence acquisition with evolutionary analysis. It contains an array of input data and multiple results explorers for visual representation; the handling and editing of sequence data, sequence alignments, inferred phylogenetic trees; and estimated evolutionary distances. The results explorers allow users to browse, edit, summarize, export, and generate publication-quality captions for their results. MEGA 4 also includes distance matrix and phylogeny explorers as well as advanced graphical modules for the visual representation of input data and output results (Tamura K, Dudley J, Nei M & Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599).


Taken together, % IDs and the generated tree (FIG. 4) indicated that potential phosphate acetyltransferases (PTA_PTB domain only) are divided in two major subfamilies. Subfamily 1 from 10.5% ID to ˜20% ID (176 sequences) and Subfamily 2 from ˜20% ID to 75.6% ID (361 sequences). The third Subfamily, of 12 sequences, has % ID ranging from 17% ID to 25% ID with respect to the L. plantarum sequence.


Based on experimentally verified sequences contained within each of the Subfamilies, Subfamily 1 and Subfamily 2 were determined to represent phosphate butyryltransferases (PTB) and phosphate acetylytransferases (PTA) respectively.


Discrimination between Subfamily 1 members and Subfamily 2 members was also performed by GroupSim analysis (Capra and Singh (2008) Bioinformatics 24: 1473-1480). The GroupSim method identifies amino acid residues that determine a protein's functional specificity. In a multiple sequence alignment (MSA) of a protein family whose sequences are, divided into multiple Subfamilies, amino acid residues that distinguish between the functional Subfamilies of sequences can be identified. The method takes a multiple sequence alignment (MSA) and known specificity groupings as input, and assigns a score to each amino acid position in the MSA. Higher scores indicate a greater likelihood that an amino acid position is a specificity determining position (SDP).


GroupSim analysis performed on the MSA of 537 sequences (divided into Subfamily 1 and Subfamily 2 by the phylogenetic analysis, above) identified highly discriminating positions. Listed in Table 11 are positions (Pos) having scores greater than to 0.7, where, a perfect score of 1.0 would indicate that all proteins within the Subfamily have the listed amino acid in the specified position and between Subfamilies the amino acid would always be different. The “Pattern” columns give the amino acid(s) in single letter code. Numbers between parentheses indicate frequency of occurrence of each amino acid at the particular position. The amino acid position number in column 1 is for the PTA protein sequence from Lactobacillus plantarum, the representative protein of group 2 with a GI#28377658 (SEQ ID NO: 1472),









TABLE 11







Highly discriminating amino acid positions for Subfamily


1 (PTB) and Subfamily 2 (PTA) from GroupSim analysis.










Pos
Score
Pattern PTB
Pattern PTA













212
0.980314
Group 1: E(173), D(2),
Group 2: S(360), N(1)




L(1)


305
0.87236
Group 1: L(152), V(11),
Group 2: D(360), Q(1)




M(5), I(5), F(3)


242
0.831201
Group 1: A(142), D(15),
Group 2: Q(361)




S(13), G(4), T(2)


208
0.776954
Group 1: L(130), I(35),
Group 2: S(355), A(6)




V(11)


125
0.705868
Group 1: K(175), R(1)
Group 2: S(215), A(85),





G(41), C(14), N(4), T(2)









An alternative structure/function characterization of the PTA and PTB subfamilies of enzymes was performed using the HMMER software package (the theory behind profile HMMs is described in R. Durbin, S. Eddy, A. Krogh, and G. Mitchison, Biological sequence analysis: probabilistic models of proteins and nucleic acids, Cambridge University Press, 1998; Krogh et al., 1994; J. Mol. Biol. 235:1501-1531), following the user guide which is available from HMMER (Janelia Farm Research Campus, Ashburn, Va.).


Using a multiple sequence alignment of the experimentally verified sequences (containing the PTA_PTB domain only) in Subfamily 2, a profile Hidden Markov Model (HMM) was created for representing Subfamily 2 members. The sequences were:


1. BAB19267.1 from Lactobacillus sanfranciscensis (SEQ ID NO: 1475)


2. NP784550.1 from Lactobacillus plantarum WCFS1 (SEQ ID NO: 1472)


3. ZP03944466.1 from Lactobacillus fermentum ATCC 14931 (SEQ ID NO: 1453)


4. NP391646.1 from Bacillus subtilis subsp. subtilis str. 168 (SEQ ID NO: 1422)


5. AAA72041.1 from Methanosarcina thermophila (SEQ ID NO: 1277)


6. ZP03152606.1 from Clostridium thermocellum DSM 4150 (SEQ ID NO: 1275)


7. NP348368.1 from Clostridium acetobutylicum ATCC 824 (SEQ ID NO: 1206)


8. YP 001394780.1 from Clostridium kluyveri DSM 555 (SEQ ID NO: 1200)


9. ZP03855267.1 from Veillonella parvula DSM 2008 (SEQ ID NO: 1159)


10. YP149725.1 from Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150 (SEQ ID NO: 1129)


The Profile HMM was built as follows: The 10 seed sequences (sequences representing experimentally verified function) that are in Subfamily 2 were aligned using Clustal X (interface to Clustal W) with default parameters. The hmmbuild program was run on each set of the aligned sequences using default parameters. hmmbuild reads the multiple sequence alignment file, builds a new Profile HMM, and saves the Profile HMM to file. Using this program an un-calibrated profile was generated from the multiple alignment for each set of subunit sequences described above.


The Profile HMM was read using hrnmcalibrate which scores a large number of synthesized random sequences with the Profile (the default number of synthetic sequences used is 5,000), fits an extreme value distribution (EVD) to the histogram of those scores, and re-saves the HMM file now including the EVD parameters. These EVD parameters (μ and λ) are used to calculate the E-values of bit scores when the profile is searched against a protein sequence database. hmmcalibrate writes two parameters into the HMM file on a line labeled “EVD”: these parameters are the μ (location) and λ (scale) parameters of an extreme value distribution (EVD) that best fits a histogram of scores calculated on randomly generated sequences of about the same length and residue composition as SWISS-PROT. This calibration was done once for the Profile HMM.


The calibrated pofile HMM for the Subfamily 2 set is provided as Table 14. The Profile HMM table gives the probability of each amino acid occurring at each position in the amino acid sequence. The amino acids are represented by the one letter code. The first line for each position reports the match emission scores: probability for each amino acid to be in that state (highest score is highlighted). The second line reports the insert emission scores, and the third line reports on state transition scores: M→M, M→i, M→D; I→M, I→I; D→M, D→D; B→M; M→E. Table 14 shows that in the Subfamily 2 profile HMM, methionine has a 3792 ans 4481 probability of being in the first two positions.


The Subfamily 2 profile HMM was evaluated using, hmmsearch, with the Z parameter set to one billion, for the ability to discriminate Subfamily 1 members from those of Subfamily 2. The hmmsearch program takes the hmm file for the Subfamily 2 profile HMM and all the sequences from both Subfamilies and assigns an E-value score to each sequence. This E-value score is a measure of fit to the Profile HMM, with a lower score being a better fit. The Profile HMM distinguished Subfamily 2 members from Subfamily 1 members and there was a large margin of E-value difference between the worst scoring Subfamily 2 member (5e-34) and the best scoring Subfamily 1 member (4.3e-07). This analysis shows that the Profile HMM prepared for Subfamily 2 phosphate acetyltransferases (PTA) distinguishes PTA sequences from phosphate butyryltransferase PTB protein sequences.


Based on these analyses, 361 phosphate acetyltransferase sequences (PTA_PTB domain only) were identified and are provided in Table 12a.









TABLE 12a







SEQ ID NOs of phosphotransacetylase target gene coding regions and proteins













GENBANK
Nucleic
GENBANK
Amino




Nucleotide
Acid
Amino Acid
Acid


GI
Sequence
SEQ
Sequence
SEQ
Source


Number
Accession Information
ID NO:
Accession No.
ID NO:
Organism















255994631
complement
762
ZP_05427766.1
1123

Eubacterium




(NZ_ACON01000003.1:




saphenum




407639 . . . 408607)



ATCC 49989


223935781
NZ_ABOX02000007.1:
763
ZP_03627696.1
1124
bacterium



10458 . . . 11600



Ellin514


196232920
NZ_ABVL01000018.1:
764
ZP_03131770.1
1125

Chthoniobacter




134406 . . . 135449




flavus








Ellin428


187735919
NC_010655.1:
765
YP_001878031.1
1126

Akkermansia




1714393 . . . 1715484




muciniphila








ATCC BAA-835


237732443
complement
766
ZP_04562924.1
1127

Citrobacter




(NZ_GG657366.1:



sp. 30_2



2437071 . . . 2438087


157144617
NC_009792.1:
767
YP_001451936.1
1128

Citrobacter




326993 . . .328009




koseri








ATCC BAA-895


56412650
NC_006511.1
768
YP_149725.1
1129

Salmonella




473496 . . . 474512




enterica subsp.









enterica serovar








Paratyphi A str. ATCC 9150


161502384
NC_010067.1:
769
YP_001569496.1
1130

Salmonella




425125 . . . 426141




enterica subsp.









arizonae








serovar 62:z4, z23:--


16130383
complement
770
NP_416953.1
1131

Escherichia




(NC_000913.2:




coli str.




257051 . . . 571527)



K-12 substr. MG1655


238895918
complement
771
YP_002920654.1
1132

Klebsiella




(NC_012731.1:




pneumoniae




3863205 . . . 3864221)



NTUH-K2044


238794182
complement
772
ZP_04637797.1
1133

Yersinia




(NZ_AALF02000025.1:




intermedia




17041 . . . 18039)



ATCC 29909


90414632
complement
773
ZP_01222604.1
1134

Photobacterium




(NZ_AAPH01000046.1:




profundum




12550 . . . 13527



3TCK


163749608
complement
774
ZP_02156855.1
1135

Shewanella




(NZ_ABIC01000008.1:




benthica




73942 . . . 74913)



KT99


120554157
NC_008740.1:
775
YP_958508.1
1136

Marinobacter




1389827 . . . 1390810




aquaeolei








VT8


51246887
complement
776
YP_066771.1
1137

Desulfotalea




(NC_006138.1:




psychrophila




3433697 . . . 3434677)



LSv54


226362753
complement
777
YP_002780531.1
1138

Rhodococcus




(NC_012522.1:




opacus




3613049 . . . 3614074)



B4


111020534
complement
778
YP_703506.1
1139

Rhodococcus




(NC_008268.1:




jostii




3751557 . . . 3752585)



RHA1


256669010
NZ_ACEV01000044.1:
779
ZP_05479963.1
1140

Streptomyces




80096 . . . 81085



sp. AA4


226227292
complement
780
YP_002761398.1
1141

Gemmatimonas




(NC_012489.1:




aurantiaca




2203452 . . . 2204474)



T-27


239627158
complement
781
ZP_04670189.1
1142

Clostridiales




(NZ_DS990260.1:




bacterium




924717 . . . 925736)



1_7_47FAA


256753163
complement
782
ZP_05493958.1
1143

Clostridium




(NZ_ACXX01000001.1:




papyrosolvens




167634 . . . 168635)



DSM 2782


257063834
NC_013165.1:
783
YP_003143506.1
1144

Slackia




1278820 . . 1279818




heliotrinireducens








DSM 20476


254477436
NZ_DS999054.1:
784
ZP_05090822.1
1145

Ruegeria




3384063 . . . 3384914



sp. R11


126732220
complement
785
ZP_01748021.1
1146

Sagittula




(NZ_AAYA01000016.1:




stellata




68052 . . . 68996)



E-37


19704507
complement
786
NP_604069.1
1147

Fusobacterium




(NC_003454.1:




nucleatum subsp.




1833702 . . . 1834715)




nucleatum








ATCC 25586


260494604
NZ_GG704456.1:
787
ZP_05814734.1
1148

Fusobacterium




222376 . . . 223389



sp. 3_1_33


262067001
complement
788
ZP_06026613.1
1149

Fusobacterium




(NZ_ACJY01000064.1:




periodonticum




9865 . . . 10869)



ATCC 33693


257452333
complement
789
ZP_05617632.1
1150

Fusobacterium




(NZ_ACDD01000037.1:



sp. 3_1_5R



1250 . . . 2263)


257463639
NZ_ACDG01000104.1:
790
ZP_05628030.1
1151

Fusobacterium




17109 . . . 18122



sp. D12


253583748
complement
791
ZP_04860946.1
1152

Fusobacterium




(NZ_GG696122.1:




varium




645905 . . . 646912)



ATCC 27725


237736963
NZ_GG657909.1:
792
ZP_04567444.1
1153

Fusobacterium




489336 . . . 490343




mortiferum








ATCC 9817


157736754
complement
793
YP_001489437.1
1154

Arcobacter




(NC_009850.1:




butzleri




500921 . . . 501916)



RM4018


257125122
NC_013192.1:
794
YP_003163236.1
1155

Leptotrichia




327731 . . . 328735




buccalis








C-1013-b


260891157
NZ_ACVB02000026.1:
795
ZP_05902420.1
1156

Leptotrichia




170989 . . . 171993




hofstadii








F0254


262037878
complement
796
ZP_06011308.1
1157

Leptotrichia




(NZ_ADAD01000064.1:




goodfellowii




9188 . . . 10195)



F0264


229859891
NZ_ABUT01000004.1:
797
ZP_04479548.1
1158

Streptobacillus




63190 . . . 64215




moniliformis








DSM 12112


227371784
NZ_ABVB01000002.1:
798
ZP_03855267.1
1159

Veillonella




260421 . . . 261419




parvula








DSM 2008


227498373
complement
799
ZP_039285211
1160

Acidaminococcus




(NZ_ACGB01000001.1:



sp. D21



109630 . . . 110643)


42525561
NC_002967.9:
800
NP_970659.1
1161

Treponema




48816 . . . 49823




denticola








ATCC 35405


257456313
NZ_ACYH01000011.1:
801
ZP_05621510.1
1162

Treponema




210840 . . . 211847




vincentii








ATCC 35580


15639088
NC_000919.1:
802
NP_218534.1
1163

Treponema




102879 . . . 103889




pallidum subsp.









pallidum








str. Nichols


228000316
complement
803
ZP_04047318.1
1164

Brachyspira




(NZ_ABTG01000001.1:




murdochii




1179041 . . . 1180048)



DSM 12563


225619252
NC_012225.1:
804
YP_002720478.1
1165

Brachyspira




340269 . . . 341276




hyodysenteriae








WA1


218960931
complement
805
YP_001740706.1
1166

Candidatus




(NS_000195.1:




Cloacamonas




716420. . . 717424)




acidaminovorans



239878221
complement
806
EER05013.1
1167

Perkinsus




(join(GG681098.1:




marinus




49679 . . . 49966,



ATCC 50983



GG681098.1:



50017 . . . 50325,



GG681098.1:



50380 . . . 50442,



GG681098.1:



50494 . . . 50605,



GG681098.1:



50656 . . . 50780,



GG681098.1:



50826 . . . 50908,



GG681098.1:



50958 . . . 51039))


119953373
NC_008710.1:
807
YP_945582.1
1168

Borrelia




614125 . . . 615171




turicatae








91E135


187918450
NC_010673.1:
808
YP_001884013.1
1169

Borrelia




616784 . . . 617842




hermsii








DAH


203284493
NC_011229.1:
809
YP_002222233.1
1170

Borrelia




622676 . . . 623746




duttonii








Ly


224534734
complement
810
ZP_03675306.1
1171

Borrelia




(NZ_ABKB02000009.1:




spielmanii




27640 . . . 28677)



A14S


216263399
NZ_ABCU02000001.1:
811
ZP_03435394.1
1172

Borrelia




172066 . . . 173103




afzelii








ACA-1


219685198
NZ_ABPZ02000001.1:
812
ZP_03540018.1
1173

Borrelia




172004 . . . 173041




garinii








Far04


224532296
NZ_ABCY02000001.1:
813
ZP_03672928.1
1174

Borrelia




609419 . . . 610456




valaisiana








VS116


15594934
NC_001318.1:
814
NP_212723.1
1175

Borrelia




608020 . . . 609078




burgdorferi








B31


189485346
NS_000191.1:
815
YP_001956287.1
1176
uncultured



518918 . . . 519919



Termite group







1 bacterium







phylotype Rs-D17


42560817
NC_005364.2:
816
NP_975268.1
1177

Mycoplasma




308545 . . . 309513




mycoides subsp.









mycoides








SC str. PG1


83319483
NC_007633.1:
817
YP_424216.1
1178

Mycoplasma




277239 . . . 278207




capricolum subsp.









capricolum








ATCC 27343


50364858
NC_006055.1:
818
YP_053283.1
1179

Mesoplasma




58892 . . . 59860




florum








L1


110005214
complement
819
CAK99540.1
1180

Spiroplasma




(AM285317.1:




citri




14153 . . . 15130)


12045155
complement
820
NP_072966.1
1181

Mycoplasma




(NC_000908.2:




genitalium




368733 . . . 369695)



G37


13508167
complement
821
NP_110116.1
1182

Mycoplasma




(NC_000912.1:




pneumoniae




515605 . . . 516567)



M129


31544825
complement
822
NP_853403.1
1183

Mycoplasma




(NC_004829.1:




gallisepticum




851083 . . . 852075)



R


26553955
complement
823
NP_757889.1
1184

Mycoplasma




(NC_004432.1:




penetrans




640803 . . . 641777)



HF-2


54020554
complement
824
YP_116016.1
1185

Mycoplasma




(NC_006360.1:




hyopneumoniae




638554 . . . 639507)



232


240047219
NC_012806.1:
825
YP_002960607.1
1186

Mycoplasma




88435 . . . 89406




conjunctivae



148377406
NC_009497.1:
826
YP_001256282.1
1187

Mycoplasma




159649 . . . 160605




agalactiae








PG2


238809713
complement
827
BAH69503.1
1188

Mycoplasma




(AP009608.1:




fermentans




242111 . . . 243064)



PG18


71894663
complement
828
YP_278771.1
1189

Mycoplasma




(NC_007294.1:




synoviae




757812 . . . 758771)



53


15828708
NC_002771.1:
829
NP_326068.1
1190

Mycoplasma




274992 . . . 275948




pulmonis








UAB CTIP


47459003
NC_006908.1:
830
YP_015865.1
1191

Mycoplasma




230100 . . . 231068




mobile








163K


148377754
NC_009497.1:
831
YP_001256630.1
1192

Mycoplasma




572993 . . . 573967




agalactiae








PG2


116515056
NC_008513.1:
832
YP_802685.1
1193

Buchnera




131608 . . . 132594




aphidicola str.








Cc (Cinara








cedri)



187934490
NC_010674.1:
833
YP_001885432.1
1194

Clostridium




1263289 . . . 1264287




botulinum








B str. Eklund 17B


150016048
NC_009617.1:
834
YP_001308302.1
1195

Clostridium




1384403 . . . 1385404




beijerinckii








NCIMB 8052


254519224
complement
835
ZP_05131280.1
1196

Clostridium




(NZ_EQ999773.1:



sp. 7_2_43FAA



2015491 . . . 2016492)


182417251
NZ_ABDT01000035.2:
836
ZP_02948604.1
1197

Clostridium




9769 . . . 10770




butyricum








5521


18310707
complement
837
NP_562641.1
1198

Clostridium




(NC_003366.1:




perfringens




2001712 . . . 2002719)



str. 13


255524273
complement
838
ZP_05391232.1
1199

Clostridium




(NZ_ACVI01000014.1:




carboxidivorans




63543 . . . 64547)



P7


153954015
NC_009706.1:
839
YP_001394780.1
1200

Clostridium




1428554 . . . 1429555




kluyveri








DSM 555


187778946
NZ_ABKW02000004.1:
840
ZP_02995419.1
1201

Clostridium




733017 . . . 734015




sporogenes








ATCC 15579


28210926
NC_004557.1:
841
NP_781870.1
1202

Clostridium




1326340 . . . 1327359




tetani








E88


253681395
NZ_ACSJ01000007.1:
842
ZP_04862192.1
1203

Clostridium




344343 . . . 345338




botulinum








D str. 1873


118444574
complement
843
YP_878298.1
1204

Clostridium




(NC_008593.1:




novyi




1416375 . . . 1417373)



NT


242260238
NZ_ACPD01000011.1:
844
ZP_04804960.1
1205

Clostridium




83320 . . . 84318




cellulovorans








743B


15895019
NC_003030.1:
845
NP_348368.1
1206

Clostridium




1890289 . . . 1891290




acetobutylicum








ATCC 824


169247670
EU313773.1:
846
ACA51668.1
1207

Thermoanaerobacterium




40 . . . 1026




saccharolyticum



255257449
NZ_ACVG01000034.1:
847
ZP_05336886.1
1208

Thermoanaerobacterium




8635 . . . 9621




thermosaccharolyticum








DSM 571


20807926
complement
848
NP_623097.1
1209

Thermoanaerobacter




(NC_003869.1:




tengcongensis




1451520 . . . 1452515)



MB4


167040369
complement
849
YP_001663354.1
1210

Thermoanaerobacter




(NC_010320.1:



sp. X514



1738259 . . . 1739257)


220931863
NC_011899.1:
850
YP_002508771.1
1211

Halothermothrix




1110901 . . . 1111899




orenii








H 168


258514457
complement
851
YP_003190679.1
1212

Desulfotomaculum




(NC_013216.1:




acetoxidans




1194895 . . . 1195899)



DSM 771


188586231
complement
852
YP_001917776.1
1213

Natranaerobius




(NC_010718.1:




thermophilus




1692944 . . . 1693942)



JW/NM-WN-LF


78044760
complement
853
YP_360288.1
1214

Carboxydothermus




(NC_007503.1:




hydrogenoformans




1302969 . . . 1303973)



Z-2901


262295620
complement
854
EEY83551.1
1215

Bacteroides




(GG705150.1:



sp. 2_1_33B



648642 . . .649655)


154494088
complement
855
ZP_02033408.1
1216

Parabacteroides




(NZ_AAXE02000107.1:




merdae




241237 . . . 242250)



ATCC 43184


34540818
complement
856
NP_905297.1
1217

Porphyromonas




(NC_002950.2:




gingivalis




1149763 . . . 1150773)



W83


228471187
complement
857
ZP_04056000.1
1218

Porphyromonas




(NZ_ACLR01000214.1:




uenonis




23231 . . . 24238)



60-3


229496164
NZ_ACNN01000020.1:
858
ZP_04389884.1
1219

Porphyromonas




205218 . . . 206225




endodontalis








ATCC 35406


160887812
complement
859
ZP_02068815.1
1220

Bacteroides




(NZ_AAY1302000031.1:




uniformis




6367 . . . 7386)



ATCC 8492


218131945
NZ_ABVO01000052.1:
860
ZP_03460749.1
1221

Bacteroides




25694 . . . 26710




eggerthii








DSM 20697


224536405
complement
861
ZP_03676944.1
1222

Bacteroides




(NZ_ACCH01000118.1:




cellulosilyticus




1796 . . . 2812)



DSM 14838


53711769
NC_006347.1:
862
YP_097761.1
1223

Bacteroides




557297 . . . 558316




fragilis








YCH46


237715344
complement
863
ZP_04545825.1
1224

Bacteroides




(NZ_EQ973249.1:



sp. D1



217217 . . . 218236)


224025178
NZ_ACBW01000140.1:
864
ZP_03643544.1
1225

Bacteroides




3350 . . . 4369




coprophilus








DSM 18228


198274546
NZ_ABQC02000011.1:
865
ZP_03207078.1
1226

Bacteroides




44269 . . . 45279




plebeius








DSM 17135


150003111
NC_009614.1:
866
YP_001297855.1
1227

Bacteroides




740818 . . . 741831




vulgatus








ATCC 8482


258649233
complement
867
ZP_05736702.1
1228

Prevotella




(NZ_ACIJ02000031.1:




tannerae




14596 . . . 15612)



ATCC 51259


261881160
NZ_ACKS01000109.1:
868
ZP_06007587.1
1229

Prevotella




4227 . . . 5276




bergensis








DSM 17361


260593477
NZ_ACVA01000073.1:
869
ZP_05858935.1
1230

Prevotella




31053 . . . 32099




veroralis








F0319


260910323
complement
870
ZP_05916997.1
1231

Prevotella




(NZ_ACZS01000043.1:



sp. oral taxon 472



34220 . . . 35257)



str. F0295


212550465
complement
871
YP_002308782.1
1232

Candidatus




(NC_011565.1:




Azobacteroides




126538 . . . 127539)




pseudotrichonymphae








genomovar. CFP2


114566305
NC_008346.1:
872
YP_753459.1
1233

Syntrophomonas




872558 . . . 873550




wolfei








subsp. wolfei







str. Goettingen


139437229
NZ_AAVN02000001.1:
873
ZP_01771389.1
1234

Collinsella




368246 . . . 369226




aerofaciens








ATCC 25986


210631306
complement
874
ZP_03296849.1
1235

Collinsella




(NZ_ABXJ01000041.1:




stercoris




3328 . . . 4308)



DSM 13279


229814970
complement
875
ZP_04445308.1
1236

Collinsella




(NZ_ABXH02000002.1:




intestinalis




65772 . . . 66770)



DSM 13280


221194458
complement
876
ZP_03567515.1
1237

Atopobium




(NZ_ACFE01000001.1:




rimae




86128 . . . 87273)



ATCC 49626


257784450
complement
877
YP_003179667.1
1238

Atopobium




(NC_013203.1:




parvulum




723329 . . . 724309)



DSM 20469


227516084
complement
878
ZP_03946133.1
1239

Atopobium




(NZ_ACGK01000007.1:




vaginae




63717 . . . 64691



DSM 15829


227872296
NZ_ACKX01000061.1:
879
ZP_03990654.1
1240

Oribacterium




10209 . . . 11261




sinus








F0268


229824780
NZ_ACIN02000002.1:
880
ZP_04450849.1
1241

Abiotrophia




126870 . . . 127931




defectiva








ATCC 49176


260443831
NZ_ACIQ01000073.1:
881
ZP_05797601.1
1242

Oribacterium




32192 . . . 33196



sp. oral taxon







078 str. F0262


225176688
complement
882
ZP_03730247.1
1243

Clostridium




(NZ_ACFX01000006.1:



sp. M62/1



113088 . . . 114089)


253578981
complement
883
ZP_04856252.1
1244

Ruminococcus




(NZ_GG696046.1:



sp. 5_1_39BFAA



364564 . . . 365595)


153813664
NZ_AAVO02000036.1:
884
ZP_01966332.1
1245

Ruminococcus




4823 . . . 5992




obeum








ATCC 29174


255281061
complement
885
ZP_05345616.1
1246

Bryantella




(NZ_ACCL02000005.1:




formatexigens




162813 . . . 163811)



DSM 14469


225571965
NZ_ACBZ01000008.1:
886
ZP_03780829.1
1247

Blautia




1408 . . . 2442




hydrogenotrophica








DSM 10507


210612569
NZ_ABWO01000095.2:
887
ZP_03289360.1
1248

Clostridium




3132 . . . 4127




nexile








DSM 1787


154505354
complement
888
ZP_02042092.1
1249

Ruminococcus




(NZ_AAYG02000022.1:




gnavus




50151 . . . 51146)



ATCC 29149


197303064
NZ_ABOU02000039.1:
889
ZP_03168112.1
1250

Ruminococcus




71843 . . . 72838




lactaris








ATCC 29176


153816169
complement
890
ZP_01968837.1
1251

Ruminococcus




(NZ_AAVP02000015.1:




torques




36559 . . . 37554)



ATCC 27756


167758299
complement
891
ZP_02430426.1
1252

Clostridium




(NZ_ABFY02000009.1:




scindens




238358 . . . 239380)



ATCC 35704


225570721
NZ_ABYI02000032.1:
892
ZP_03779744.1
1253

Clostridium




3477 . . . 4499




hylemonae








DSM 15053


166031766
NZ_AAXA02000013.1:
893
ZP_02234595.1
1254

Dorea




54410 . . . 55414




formicigenerans








ATCC 27755


153853264
complement
894
ZP_01994673.1
1255

Dorea




(NZ_AAXB02000002.1:




longicatena




216862 . . . 217857)



DSM 13814


160879474
NC_010001.1:
895
YP_01558442.1
1256

Clostridium




1657582 . . . 1658577




phytofermentans








ISDg


239624054
complement
896
ZP_04667085.1
1257

Clostridiales




(NZ_DS990263.1:




bacterium




658578 . . . 659573)



1_7_47FAA


160938034
complement
897
ZP_02085391.1
1258

Clostridium




(NZ_ABCC02000027.1:




bolteae




52316 . . . 53311)



ATCC BAA-613


260437037
complement
898
ZP_05790853.1
1259

Butyrivibrio




(NZ_ABWN01000017.1:




crossotus




33488 . . . 34483)



DSM 2876


154483586
complement
899
ZP_02026034.1
1260

Eubacterium




(NZ_AAVL02000033.1:




ventriosum




74910 . . . 75941)



ATCC 27560


238916996
complement
900
YP_002930513.1
1261

Eubacterium




(NC_012778.1:




eligens




1076225 . . . 1077220)



ATCC 27750


242309058
NZ_DS990446.1:
901
ZP_04808213.1
1262

Helicobacter




108718 . . . 109737




pullorum








MIT 98-5489


224418114
complement
902
ZP_03656120.1
1263

Helicobacter




(NZ_ABQS01000024.1:




canadensis




18744 . . . 19745



MIT 98-5491


237752737
NZ_GG661974.1:
903
ZP_04583217.1
1264

Helicobacter




463241 . . . 464236




winghamensis








ATCC BAA-430


32266808
complement
904
NP_860840.1
1265

Helicobacter




(NC_004917.1:




hepaticus




1266998 . . . 1267993)



ATCC 51449


224436915
complement
905
ZP_03657896.1
1266

Helicobacter




(NZ_ABQT01000013.1:




cinaedi




10506 . . . 11522)



CCUG 18818


167745652
complement
906
ZP_02417779.1
1267

Anaerostipes




(NZ_ABAX03000002.1:




caccae




101957 . . . 102961)



DSM 14662


167765558
complement
907
ZP_02437622.1
1268

Clostridium




(NZ_ABGC03000004.1:



sp. SS2/1



50807 . . . 51820)


163814038
NZ_ABEY02000003.1:
908
ZP_02205430.1
1269

Coprococcus




15727 . . . 16794




eutactus








ATCC 27759


168334441
complement
909
ZP_02692616.1
1270

Epulopiscium




(NZ_ABEQ01000029.2:



sp. ‘N.t.



21420 . . . 22418)



morphotype B’


257791476
NC_013204.1:
910
YP_003182082.1
1271

Eggerthella




2035882 . . . 2036880




lenta








DSM 2243


256827068
complement
911
YP_003151027.1
1272

Cryptobacterium




(NC_013170.1:




curtum




735166 . . . 736167)



DSM 15641


257063929
complement
912
YP_003143601.1
1273

Slackia




(NC_013165.1:




heliotrinireducens




1407526 . . . 1408521)



DSM 20476


256757417
complement
913
ZP_05498135.1
1274

Clostridium




(NZ_ACXX01000078.1:




papyrosolvens




7706 . . . 8701)



DSM 2782


196254011
NZ_ABVG01000076.1:
914
ZP_03152606.1
1275

Clostridium




9016 . . . 10092




thermocellum








JW20


146297046
complement
915
YP_001180817.1
1276

Caldicellulosiruptor




(NC_009437.1:




saccharolyticus




2185773 . . . 2186804)



DSM 8903


349833
L23147.1:
916
AAA72041.1
1277

Methanosarcina




207 . . . 1208




thermophila



20092407
complement
917
NP_618482.1
1278

Methanosarcina




(NC_003552.1:




acetivorans




4448053 . . . 4449054)



C2A


73669327
complement
918
YP_305342.1
1279

Methanosarcina




(NC_007355.1:




barkeri




2275987 . . . 2276988)



str. Fusaro


163734840
NZ_ABIG01000010.1:
919
ZP_02142278.1
1280

Roseobacter




132890 . . . 133867




litoralis








Och 149


110678177
complement
920
YP_681184.1
1281

Roseobacter




(NC_008209.1:




denitrificans




803709 . . . 804707)



OCh 114


159044374
complement
921
YP_001533168.1
1282

Dinoroseobacter




(NC_009952.1:




shibae




1904769 . . . 1905794)



DFL 12


254512869
NZ_DS999532.1:
922
ZP_05124935.1
1283

Rhodobacteraceae




435221 . . . 436255




bacterium








KLH11


260432366
complement
923
ZP_05786337.1
1284

Silicibacter




(NZ_GG704596.1:




lacuscaerulensis




1443685 . . . 1444707)



ITI-1157


150376990
NC_009620.1:
924
YP_001313586.1
1285

Sinorhizobium




1371590 . . . 1372624




medicae








WSM419


16264720
NC_003078.1:
925
NP_437512.1
1286

Sinorhizobium




1058537 . . . 1059529




meliloti








1021


239833801
complement
926
ZP_04682129.1
1287

Ochrobactrum




(NZ_ACQA01000002.1:




intermedium




595918 . . . 596886)



LMG 3301


153010822
complement
927
YP_001372036.1
1288

Ochrobactrum




(NC_00968.1:




anthropi




862920 . . . 863897)



ATCC 49188


187919084
complement
928
YP_001888115.1
1289

Burkholderia




(NC_010676.1:




phytofirmans




423371 . . . 424405)



PsJN


91779405
NC_007952.1:
929
YP_554613.1
1290

Burkholderia




2605754 . . . 2606791




xenovorans








LB400


186470979
NC_010625.1:
930
YP_001862297.1
1291

Burkholderia




763673 . . . 764704




phymatum








STM815


73537607
complement
931
YP_297974.1
1292

Ralstonia




(NC_007348.1:




eutropha




372720 . . . 373757)



JMP134


194292312
NC_010530.1:
932
YP_002008219.1
1293

Cupriavidus




1692071 . . . 1693105




taiwanensis



161521061
NC_010086.1:
933
YP_001584488.1
1294

Burkholderia




1600765 . . . 1601856




multivorans








ATCC 17616


206563034
complement
934
YP_002233797.1
1295

Burkholderia




(NC_011001.1:




cenocepacia




1288493 . . . 1289527)



J2315


90412230
complement
935
ZP_01220235.1
1296

Photobacterium




(NZ_AAPH01000013.1:




prorundum




616 . . . 1653)



3TCK


224825256
complement
936
ZP_03698361.1
1297

Lutiella




(NZ_ACIS01000005.1:




nitroferrum




7593 . . . 8612)



2002


148973982
complement
937
ZP_01811515.1
1298

Vibrionales




(NZ_AAZW01000001.1:




bacterium




115872 . . . 116945)



SWAT-3


84385317
complement
938
ZP_00988349.1
1299

Vibrio




(NZ_AAMR01000001.1:




splendidus




227808 . . . 228881)



12B01


149187938
NZ_ABCH01000003.1:
939
ZP_01866234.1
1300

Vibrio




163877 . . . 164926




shilonii








AK1


260776268
complement
940
ZP_05885163.1
1301

Vibrio




(NZ_ACZN01000015.1:




coralliilyticus




316917 . . . 317966)



ATCC BAA-450


45862014
AY498613.1:
941
AAS78789.1
1302

Paracoccus




8897 . . . 9853




denitrificans



77404622
NC_007488.1:
942
YP_345196.1
1303

Rhodobacter




38175 . . . 39173




sphaeroides








2.4.1


23630309
AY134843.1:
943
AAN08490.1
1304

Castellaniella




2180 . . . 3148




defragrans



83952615
NZ_AALY01000004.1:
944
ZP_00961345.1
1305

Roseovarius




6833 . . . 7819




nubinhibens








ISM


56698382
complement
945
YP_168755.1
1306

Ruegeria




(NC_003911.11:




pomeroyi




3772593 . . . 3773606)



DSS-3


149912659
NZ_ABCR01000001.1:
946
ZP_01901193.1
1307

Roseobacter




262172 . . . 263161



sp. AzwK-3b


126736835
complement
947
ZP_01752570.1
1308

Roseobacter




(NZ_AAYC01000001.1:



sp. SK209-2-6



23010 . . . 24029)


163732628
complement
948
ZP_02140073.1
1309

Roseobacter




(NZ_ABIG01000003.1:




litoralis




152433 . . . 153374)



Och 149


89055338
NC_007802.1:
949
YP_510789.1
1310

Jannaschia




2864232 . . . 2865239



sp. CCS1


254459737
NZ_DS995276.1:
950
ZP_05073153.1
1311

Rhodobacterales




713420 . . . 714472




bacterium








HTCC2083


116620211
NC_008536.1:
951
YP_822367.1
1312

Candidatus




1336713 . . . 1337708




Solibacter









usitatus








Ellin6076


95930364
NZ_AAEW02000012.1:
952
ZP_01313101.1
1313

Desulfuromonas




74280 . . . 75281




acetoxidans








DSM 684


77920135
NC_007498.2:
953
YP_357950.1
1314

Pelobacter




2984046 . . . 2985047




carbinolicus








DSM 2380


222054722
complement
954
YP_002537084.1
1315

Geobacter




(NC_011979.1:



sp. FRC-32



1793784 . . . 1794785)


148265418
NC_009483.1:
955
YP_001232124.1
1316

Geobacter




3992460 . . . 2993461




uraniireducens








Rf4


39997800
NC_002939.4:
956
NP_953751.1
1317

Geobacter




2984470 . . . 2985471




sulfurreducens








PCA


78222253
complement
957
YP_384000.1
1318

Geobacter




(NC_007517.1:




metallireducens




1150703 . . . 1151704)



GS-15


118579718
complement
958
YP_900968.1
1319

Pelobacter




(NC_008609.1:




propionicus




1359173 . . . 1360177)



DSM 2379


189424275
complement
959
YP_001951452.1
1320

Geobacter




(NC_010814.1:




lovleyi




1256052 . . . 1257053)



SZ


255059775
NZ_ACPJ01000030.1:
960
ZP_05311922.1
1321

Geobacter




50425 . . . 51426



sp. M18


253700569
complement
961
YP_003021758.1
1322

Geobacter




(NC_012918.1:



sp. M21



2257017 . . . 2258018)


77920440
complement
962
YP_358255.1
1323

Pelobacter




(NC_007498.2:




carbinolicus




3332135 . . . 3333136)



DSM 2380


227423754
NZ_ABTN01000011.1:
963
ZP_03906856.1
1324

Denitrovibrio




41883 . . . 42872




acetiphilus








DSM 12809


193215894
NC_011026.1:
964
YP_001997093.1
1325

Chloroherpeton




2629909 . . . 2630916




thalassium








ATCC 35110


150386298
complement
965
ZP_01924858.1
1326

Victivallis




(NZ_ABDE01000122.1:




vadensis




6609 . . . 7619)



ATCC BAA-548


217034411
NZ_ABSX01000018.1:
966
ZP_03439825.1
1327

Helicobacter




29972 . . . 31570




pylori








98-10


254779508
complement
967
YP_003057614.1
1328

Helicobacter




(NC_012973.1:




pylori




876982 . . . 878538)



B38


188527730
complement
968
YP_001910417.1
1329

Helicobacter




(NC_010698.2:




pylori




936737 . . . 938293)



Shi470


15611908
complement
969
NP_223559.1
1330

Helicobacter




(NC_000921.1:




pylori




920263 . . . 921822)



J99


109947805
complement
970
YP_665033.1
1331

Helicobacter




(NC_008229.1:




acinonychis




1105678 . . . 1107201)



str. Sheeba


148926656
NZ_AASY01000008.1:
971
ZP_01810337.1
1332

Campylobacter




52243 . . . 53778




jejuni subsp.









jejuni








CG8486


57167700
complement
972
ZP_00366840.1
1333

Campylobacter




(NZ_AAFL01000001.1:




coli




232544 . . . 234046)



RM2228


57242590
complement
973
ZP_00370527.1
1334

Campylobacter




(NZ_AAFJ01000004.1:




upsaliensis




6136 . . . 7638)



RM3195


222823645
NC_012039.1:
974
YP_002575219.1
1335

Campylobacter




612447 . . . 613916




lari








RM2100


154148075
complement
975
YP_001406718.1
1336

Campylobacter




(NC_009714.1:




hominis




1098161 . . . 1099597)



ATCC BAA-381


257459711
NZ_ACYG01000019.1:
976
ZP_05624820.1
1337

Campylobacter




245785 . . . 247383




gracilis








RM3268


118475502
complement
977
YP_891988.1
1338

Campylobacter




(NC_008599.1:




fetus subsp.




824533 . . . 825927)




fetus 82-40



157164211
NC_009802.1:
978
YP_001466901.1
1339

Campylobacter




1056736 . . . 1058103




concisus








13826


154173700
complement
979
YP_001408221.1
1340

Campylobacter




(NC_009715.1:




curvus




947742 . . . 949112)



525.92


255322202
NZ_ACVQ01000017.1:
980
ZP_05363348.1
1341

Campylobacter




43696 . . . 45081




showae








RM3277


225351910
NZ_ABXX02000003.1:
981
ZP_03742933.1
1342

Bifidobacterium




117844 . . . 119514




pseudocatenulatum








DSM 20438


171743080
complement
982
ZP_02918887.1
1343

Bifidobacterium




(NZ_ABIX02000002.1:




dentium




2348529 . . . 2350229)



ATCC 27678


154487476
complement
983
ZP_02028883.1
1344

Bifidobacterium




(NZ_AAX1302000028.1:




adolescentis




209751 . . . 211442)



L2-32


229817818
complement
984
ZP_04448100.1
1345

Bifidobacterium




(NZ_ABYS02000004.1:




angulatum




899528 . . . 901234)



DSM 20098


223467350
complement
985
ZP_03618886.1
1346

Bifidobacterium




(NZ_ACCG01000014.1:




breve




93417 . . . 95159)



DSM 20213


227546035
NZ_ACHI01000009.1:
986
ZP_03976084.1
1347

Bifidobacterium




13043 . . . 14755




longum subsp.









infantis








ATCC 55813


213692597
NC_011593.1:
987
YP_002323183.1
1348

Bifidobacterium




1898224 . . . 1899936




longum subsp.









infantis








ATCC 15697


224282865
complement
988
ZP_03646187.1
1349

Bifidobacterium




(NZ_ABQP01000009.1:




bifidum




208984 . . . 210654)



NCIMB 41171


227507562
NZ_ACGF01000124.1:
989
ZP_03937611.1
1350

Gardnerella




44384 . . . 46048




vaginalis








ATCC 14019


183601499
complement
990
ZP_02962869.1
1351

Bifidobacterium




(NZ_ABOT01000001.1:




animalis subsp.




192971 . . . 194653)




lactis








HN019


261337301
complement
991
ZP_05965185.1
1352

Bifidobacterium




(NZ_ABXB03000001.1:




gallicum




137782 . . . 139455)



DSM 20093


154507766
NZ_AAYI02000004.1:
992
ZP_02043408.1
1353

Actinomyces




231567 . . . 233045




odontolyticus








ATCC 17982


227494860
complement
993
ZP_03925176.1
1354

Actinomyces




(NZ_ACFG01000030.1:




coleocanis




86700 . . . 88295)



DSM 15436


19553946
complement
994
NP_601948.1
1355

Corynebacterium




(NC_003450.3:




glutamicum




2936506 . . . 2937891)



ATCC 13032


25029147
complement
995
NP_739201.1
1356

Corynebacterium




(NC_004369.1:




efficiens




2758982 . . . 2760496)



YS-314


38234612
complement
996
NP_940379.1
1357

Corynebacterium




(NC_002935.2:




diphtheriae




2103677 . . . 2105128)



NCTC 13129


252124104
complement
997
ZP_04835255.1
1358

Corynebacterium




(NZ_ACSH01000003.1:




matruchotii




61905 . . . 63305)



ATCC 14266


227489285
NZ_ABYP01000094.1:
998
ZP_03919601.1
1359

Corynebacterium




61648 . . . 63048




glucuronolyticum








ATCC 51867


258561950
NZ_ACLJ01000070.1:
999
ZP_05708623.1
1360

Corynebacterium




13162 . . . 14523




genitalium








ATCC 33030


227547861
NZ_ACHJ01000017.1:
1000
ZP_03977910.1
1361

Corynebacterium




9541 . . . 10899




lipophiloflavum








DSM 44291


227502015
NZ_ACGD01000004.1:
1001
ZP_03932064.1
1362

Corynebacterium




82938 . . . 84305




accolens








ATCC 49725


255325798
NZ_ACVP01000037.1:
1002
ZP_05366890.1
1363

Corynebacterium




5746 . . . 7107




tuberculostearicum








SK141


227505901
NZ_ACGE01000122.1:
1003
ZP_03935950.1
1364

Corynebacterium




37468 . . . 38826




striatum








ATCC 6940


227834110
complement
1004
YP_002835817.1
1365

Corynebacterum




(NC_012590.1:




aurimucosum




2492850 . . . 2494214)



ATCC 700975


68535315
NC_007164.1:
1005
YP_250020.1
1366

Corynebacterium




307337 . . . 308848




jeikeium








K411


172041418
complement
1006
YP_001801132.1
1367

Corynebacterium




(NC_010545.1:




urealyticum




2018026 . . . 2019369)



DSM 7109


237786249
complement
1007
YP_002906954.1
1368

Corynebacterium




(NC_012704.1:




kroppenstedtii




1975731 . . . 1977287)



DSM 44385


213965099
complement
1008
ZP_03393297.1
1369

Corynebacterium




(NZ_ABZU01000003.1:




amycolatum




128017 . . . 129543)



SK46


225075788
complement
1009
ZP_03718987.1
1370

Neisseria




(NZ_ACEN01000020.1:




flavescens




5387 . . . 6889)



NRL30031/H210


255067101
NZ_ACKO02000012.1:
1010
ZP_05318956.1
1371

Neisseria




64851 . . . 66353




sicca








ATCC 29256


161869564
complement
1011
YP_001598731.1
1372

Neisseria




(NC_010120.1:




meningitidis




603202 . . . 604836)



053442


238022551
NZ_ACJW02000003.1:
1012
ZP_04602977.1
1373

Kingella




751672 . . . 753150




oralis








ATCC 51147


83592714
complement
1013
YP_426466.1
1374

Rhodospirillum




(NC_007643.1:




rubrum




1625036 . . . 1626802)



ATCC 11170


32490929
complement
1014
NP_871183.1
1375

Wigglesworthia




(NC_004344.2:




glossinidia




212680 . . . 214818)



endosymbiont







of Glossina








brevipalpis



27904667
NC_004545.1:
1015
NP_777793.1
1376

Buchnera




186377 . . . 188524




aphidicola str.








Bp (Baizongia








pistaciae)



261415723
complement
1016
YP_003249406.1
1377

Fibrobacter




(NC_013410.1:




succinogenes subsp.




1639393 . . . 1640796)




succinogenes S85



219556226
NZ_ABQH01000061.1:
1017
ZP_03535302.1
1378

Mycobacterium




<3 . . . 1196




tuberculosis








T17


228471665
complement
1018
ZP_04056438.1
1379

Capnocytophaga




(NZ_ACLQ01000003.1:




gingivalis




154599 . . . 155657)



ATCC 33624


256370675
NC_013123.1:
1019
YP_003108500.1
1380

Candidatus




116952 . . . 117950




Sulcia









muelleri








SMDSEM


6685772
X89084.1:
1020
P77844
1381

Corynebacterium




1009 . . . 199




glutamicum



227876041
NZ_ACKW01000045.1:
1021
ZP_03994160.1
1382

Mobiluncus




33898 . . . 34887




mulieris








ATCC 35243


227492324
NZ_ACCQ01000004.1:
1022
ZP_03922640.1
1383

Mobiluncus




214416 . . . 215417




curtisii








ATCC 43063


225027017
NZ_ACEP01000064.1:
1023
ZP_03716209.1
1384

Eubacterium




10650 . . . 11609




halli








DSM 3353


225028951
complement
1024
ZP_03718143.1
1385

Eubacterium




(NZ_ACEP01000172.1:




hallii




22364 . . . 23416)



DSM 3353


257438679
complement
1025
ZP_05614434.1
1386

Faecalibacterium




(NZ_ACOP02000029.1:




prausnitzii




99 . . . 1124)



A2-165


154496156
complement
1026
ZP_02034852.1
1387

Bacteroides




(NZ_AAXG02000004.1:




capillosus




103391 . . . 104380)



ATCC 29799


225376322
NZ_ACFY01000086.1:
1027
ZP_03753543.1
1388

Roseburia




6940 . . . 7992




inulinivorans








DSM 16841


257414121
complement
1028
ZP_04745275.2
1389

Roseburia




(NZ_ABYJ02000202.1:




intestinalis




41125 . . . 42165)



L1-82


238923816
NC_012781.1:
1029
YP_002937332.1
1390

Eubacterium




1324280 . . . 1325263




rectale








ATCC 33656


160893459
NZ_AAYW02000007.1:
1030
ZP_02074244.1
1391

Clostridium




63870 . . . 64919



sp. L2-50


229829305
complement
1031
ZP_04455374.1
1392

Shuttleworthia




(NZ_ACIP02000002.1:




satelles




495313 . . . 496299)



DSM 14600


218282181
complement
1032
ZP_03488480.1
1393

Eubacterium




(NZ_ABYT01000061.1:




biforme




45 . . . 1016)



DSM 398


160916120
complement
1033
ZP_02078327.1
1394

Eubacterium




(NZ_ABAW02000025.1:




dolichum




71684 . . . 72694)



DSM 3991


160915347
NZ_ABAW02000020.1:
1034
ZP_02077559.1
1395

Eubacterium




14646 . . . 15638




dolichum








DSM 3991


212697404
NZ_ABXA01000047.1:
1035
ZP_03305532.1
1396

Anaerococcus




41715 . . . 42701




hydrogenalis








DSM 7454


256545936
complement
1036
ZP_05473291.1
1397

Anaerococcus




(NZ_ACXU01000022.1:




vaginalis




22101 . . . 23087)



ATCC 51170


227501001
NZ_ACGC01000115.1:
1037
ZP_03931050.1
1398

Anaerococcus




23122 . . . 24108




tetradius








ATCC 35098


257067207
complement
1038
YP_003153463.1
1399

Anaerococcus




(NC_013171.1:




prevotii




1868769 . . . 1869752)



DSM 20548


227485732
complement
1039
ZP_03916048.1
1400

Anaerococcus




(NZ_ABYO01000196.1:




lactolyticus




43814 . . . 44833)



ATCC 51172


19746077
NC_003485.1:
1040
NP_607213.1
1401

Streptococcus




903788 . . . 904783




pyogenes








MGAS8232


13622266
AE004092.1:
1041
AAK34003.1
1402

Streptococcus




923921 . . . 924916




pyogenes








M1 GAS


222153008
NC_012004.1:
1042
YP_002562185.1
1403

Streptococcus




834034 . . . 835026




uberis








0140J


225868503
NC_012470.1:
1043
YP_002744451.1
1404

Streptococcus




1034662 . . . 1035663




equi subsp.









Zooepidemicus



254997415
AP010655.1:
1044
BAH88016.1
1405

Streptococcus




1031526 . . . 1032521




mutans








NN2025


171779341
NZ_ABJK02000020.1:
1045
ZP_02920305.1
1406

Streptococcus




38474 . . . 39472




infantarius








subsp.








infantarius








ATCC BAA-102


76787123
complement
1046
YP_329798.1
1407

Streptococcus




(NC_007432.1:




agalactiae




1155758 . . . 1156750)



A909


228477151
complement
1047
ZP_04061789.1
1408

Streptococcus




(NZ_ACLO01000062.1:




salivarius




54543 . . . 55526)



SK126


55821439
complement
1048
YP_139881.1
1409

Streptococcus




(NC_006448.1:




thermophilus




1286014 . . . 1286997)



LMG 18311


237650772
NZ_ABZC01000093.1:
1049
ZP_04525024.1
1410

Streptococcus




10653 . . . 11627




pneumoniae








CCRI 1974


262282806
complement
1050
ZP_06060573.1
1411

Streptococcus




(NZ_GG7049411.1:



sp. 2_1_36FAA



11119 . . . 12096)


146318711
complement
1051
YP_001198423.1
1412

Streptococcus




(NC_009442.1:




suis




1032399 . . . 1033379)



05ZYH33


42518809
NC_005362.1:
1052
NP_964739.1
1413

Lactobacillus




788505 . . . 789482




johnsonii








NCC 533


58337025
NC_006814.3:
1053
YP_193610.1
1414

Lactobacillus




698578 . . . 699567




acidophilus








NCFM


227893214
NZ_ACGU01000037.1:
1054
ZP_04011019.1
1415

Lactobacillus




27358 . . . 28347




ultunensis








DSM 16047


227877224
NZ_ACKR01000025.1:
1055
ZP_03995297.1
1416

Lactobacillus




14947 . . . 15936




crispatus








JV-V01


260102516
NZ_ACLM01000112.1:
1056
ZP_05752753.1
1417

Lactobacillus




6924 . . . 7913




helveticus








DSM 20075


227525975
NZ_ACGQ01000041.1:
1057
ZP_03956024.1
1418

Lactobacillus




59881 . . . 60858




jensenii








JV-V16


228854857
complement
1058
ZP_04645187.1
1419

Lactobacillus




(NZ_ACOY01000013.1:




jensenii




251390 . . . 252367)



269-3


104773739
NC_008054.1:
1059
YP_618719.1
1420

Lactobacillus




547017 . . . 548006




delbrueckii subsp.









bulgaricus








ATCC 11842


259501464
NZ_ACLN01000013.1:
1060
ZP_05744366.1
1421

Lactobacillus




15438 . . . 16418




iners








DSM 13335


16080818
complement
1061
NP_391646.1
1422

Bacillus




(NC_000964.3:




subtilis subsp.




3865355 . . . 3866326)




subtilis str. 168



154687884
complement
1062
YP_001423045.1
1423

Bacillus




(NC_009725.1:




amyloliquefaciens




3590964 . . . 3591935)



FZB42


52082282
complement
1063
YP_081073.1
1424

Bacillus




(NC_006270.3:




licheniformis




3821313 . . . 3822284)



ATCC 14580


194016487
complement
1064
ZP_03055101.1
1425

Bacillus




(NZ. ABRX01000004.1:




pumilus




144981 . . . 145952)



ATCC 7061


212640578
complement
1065
YP_002317098.1
1426

Anoxybacillus




(NC_011567.1:




flavithermus




2748264 . . . 2749247)



WK1


239828646
complement
1066
YP_002951270.1
1427

Geobacillus




(NC_012793.1:



sp. WCH70



3393094 . . . 3394068)


138896990
complement
1067
YP_001127443.1
1428

Geobacillus




(NC_009328.1:




thermodenitrificans




3468960 . . . 3469934)



NG80-2


56421950
complement
1068
YP_149268.1
1429

Geobacillus




(NC_006510.1:




kaustophilus




3456185 . . . 3457165)



HTA426


149182788
NZ_ABCF01000043.1:
1069
ZP_01861251.1
1430

Bacillus




11583 . . . 12554



sp. SG-1


205375387
NZ_ABFU01000065.2:
1070
ZP_03228176.1
1431

Bacillus




12128 . . . 13099




coahuilensis








m4-4


89101108
complement
1071
ZP_01173945.1
1432

Bacillus sp.




(NZ_AAOX01000058.1:



NRRL B-14911



10738 . . . 11715)


23100477
complement
1072
NP_693944.1
1433

Oceanobacillus




(NC_004193.1:




iheyensis




3134492 . . . 3135466)



HTE831


229187615
complement
1073
ZP_04314753.1
1434

Bacillus




(NZ_ACLU01000117.1:




cereus




31676 . . . 32647)



BGSC 6E1


46908338
complement
1074
YP_014727.1
1435

Listeria




(NC_002973.6:




monocytogenes




2171357 . . . 2172334)



str. 4b F2365


229555968
NZ_ACCR01000020.1:
1075
ZP_04443757.1
1436

Listeria grayi




18426 . . . 19403



DSM 20601


15616385
complement
1076
NP_244690.1
1437

Bacillus




(NC_002570.2:




halodurans




3947889 . . . 3948881)



C-125


56965668
complement
1077
YP_177402.1
1438

Bacillus




(NC_006582.1:




clausii




4069370 . . . 4070365)



KSM-K16


229917170
complement
1078
YP_002885816.1
1439

Exiguobacterium




(NC_012673.1:



sp. AT1b



1410227 . . . 1411216)


172056261
NC_010556.1:
1079
YP_001812721.1
1440

Exiguobacterium




233988 . . . 234974




sibiricum








255-15


163762281
NZ_ABHZ01000002.1:
1080
ZP_02169346.1
1441

Bacillus




94480 . . . 95457




selenitireducens








MLS10


242372812
NZ_ACJB01000048.1:
1081
ZP_04818386.1
1442

Staphylococcus




8122 . . . 9111




epidermidis








M23864: W1


223042925
complement
1082
ZP_03612973.1
1443

Staphylococcus




(NZ_ACFR01000002.1:




capitis




330954 . . . 331943)



SK14


239636796
complement
1083
ZP_04677798.1
1444

Staphylococcus




(NZ_ACPZ01000027.:1




warneri




569915 . . . 570904)



L37603


27467277
NC_004461.1:
1084
NP_763914.1
1445

Staphylococcus




356818 . . . 357807




epidermidis








ATCC 12228


258422775
NZ_ACKI01000006.1:
1085
ZP_05685678.1
1446

Staphylococcus




980 . . . 1966




aureus








A9635


70727403
complement
1086
YP_254319.1
1447

Staphylococcus




(NC_007168.1:




haemolyticus




2403280 . . . 2404269)



JCSC1435


228475091
NZ_ACLP01000011.1:
1087
ZP_04059818.1
1448

Staphylococcus




16037 . . . 17026




hominis








SK119


150011041
EF456699.1:
1088
ABR57177.1
1449

Staphylococcus




1 . . . 987




xylosus



73663433
complement
1089
YP_302214.1
1450

Staphylococcus




(NC_007356.1:




saprophyticus subsp.




2190871 . . . 2191857)




saprophyticus








ATCC 15305


224475734
NC_012121.1:
1090
YP_002633340.1
1451

Staphylococcus




250258 . . . 251247




carnosus subsp.









carnosus








TM300


222152076
complement
1091
YP_002561236.1
1452

Macrococcus




(NC_011999.1:




caseolyticus




1968130 . . . 1969119)



JCSC5402


227514417
NZ_ACGI01000058.1:
1092
ZP_03944466.1
1453

Lactobacillus




71225 . . . 72199




fermentum








ATCC 14931


256848058
complement
1093
ZP_05553502.1
1454

Lactobacillus




(NZ_GG698804.1:




coleohominis




125094 . . . 126059)



101-4-CHN


227529580
NZ_ACGV01000117.1:
1094
ZP_03959629.1
1455

Lactobacillus




1634 . . . 2608




vaginalis








ATCC 49540


148543634
NC_009513.1:
1095
YP_001271004.1
1456

Lactobacillus




451991 . . . 452965




reuteri








DSM 20016


259502766
NZ_ACLL01000024.1:
1096
ZP_05745668.1
1457

Lactobacillus




18101 . . . 19072




antri








DSM 16041


116618560
complement
1097
YP_818931.1
1458

Leuconostoc




(NC_008531.1:




mesenteroides subsp.




1461235 . . . 1462215)




mesenteroides








ATCC 8293


170016912
NC_010471.1:
1098
YP_001727831.1
1459

Leuconostoc




577967 . . . 578959




citreum








KM20


241894748
complement
1099
ZP_04782044.1
1460

Weissella




(NZ_ACKU01000002.1:




paramesenteroides




15496 . . . 16476)



ATCC 33313


118587037
NZ_AAUV01000054.1:
1100
ZP_01544468.1
1461

Oenococcus




44038 . . . 45132




oeni








ATCC BAA-1163


259046893
complement
1101
ZP_05737294.1
1462

Granulicatella




(NZ_ACKZ01000012.:1




adiacens




86436 . . . 87425)



ATCC 49175


260584167
complement
1102
ZP_05851915.1
1463

Granulicatella




(NZ_GG703805.1:




elegans




786281 . . . 787264)



ATCC 700633


163789527
complement
1103
ZP_02183965.1
1464

Carnobacterium




(NZ_ABHH01000002.1:



sp. AT7



8081 . . . 9061)


257870102
NZ_GG670288.1:
1104
ZP_05649755.1
1465

Enterococcus




145742 . . . 146725




gallinarum








EG2


227517869
NZ_ACGL01000051.1:
1105
ZP_03947918.1
1466

Enterococcus




2376 . . . 3401




faecalis








TX0104


227552175
complement
1106
ZP_03982224.1
1467

Enterococcus




(NZ.ACHL01000118.1:




faecium




1216 . . . 2232)



TX1330


81428954
complement
1107
YP_395954.1
1468

Lactobacillus




(NC_007576.1:




sakei subsp.




1313600 . . . 1314586)




sakei 23K



229823693
NZ_ACIL02000007.1:
1108
ZP_04449762.1
1469

Catonella




6499 . . . 7482




morbi








ATCC 51271


125623617
NC_009004.1:
1109
YP_001032100.1
1470

Lactococcus




752099 . . . 753079




lactis subsp.









cremoris








MG1363


116494500
NC_008526.1:
1110
YP_806234.1
1471

Lactobacillus




981403 . . . 982380




casei








ATCC 334


28377658
NC_004567.1:
1111
NP_784550.1
1472

Lactobacillus




748192 . . . 749169




plantarum








WCFS1


116333321
NC_008497.1:
1112
YP_794848.1
1473

Lactobacillus




702374 . . . 703348




brevis








ATCC 367


227524782
complement
1113
ZP_03954831.1
1474

Lactobacillus




(NZ_ACGP01000192.1:




hilgardii




2135 . . . 3112)



ATCC 8290


11862872
AB035800.1:
1114
BAB19267.1
1475

Lactobacillus




1006 . . . 1992




sanfranciscensis



227528239
NZ_ACGS01000093.1:
1115
ZP_03958288.1
1476

Lactobacillus




64718 . . . 65695




ruminis








ATCC 25644


90962126
complement
1116
YP_536042.1
1477

Lactobacillus




(NC_007929.1:




salivarius




1183945 . . . 1184922)



UCC118


259504733
NZ_ACLK01000016.1:
1117
ZP_05747635.1
1478

Erysipelothrix




55937 . . . 56917




rhusiopathiae








ATCC 19414


116492140
NC_008525.1:
1118
YP_803875.1
1479

Pediococcus




385259 . . . 386230




pentosaceus








ATCC 25745


160946581
NZ_ABEE02000016.1:
1119
ZP_02093784.1
1480

Parvimonas




72101 . . . 73072




micra








ATCC 33270


169825312
complement
1120
YP_001692923.1
1481

Finegoldia




(NC_010376.1:




magna




1782855 . . . 1783826)



ATCC 29328


229542439
NZ_AAWV02000001.1:
1121
ZP_04431499.1
1482

Bacillus




1452854 . . . 1453825




coagulans








36D1


241888505
NZ_ACDZ02000004.1:
1122
ZP_04775813.1
1483

Gemella




11622 . . . 12602




haemolysans








ATCC 10379









In addition, 201 phosphate acetyltransferase sequences that are characterized by two domains (DRTGG and PTA_PTB) are provided in Table 12b. MSA and phylogenetic analysis were performed as described above. Percent identity with respect to experimentally verified (or human curated) sequences is equal to or larger than 40, except for 4 sequences derived from plant organisms. Furthermore, hmmer search of the 201 sequences against the profile HMM of subfamily 2 (Table 14), clearly indicates that all Group 2 sequences belong to the PTA subfamily (least significant Evalue is 4.1e-93).









TABLE 12b







SEQ ID NOs of phosphotransacetylase target gene coding regions and proteins













GENBANK
Nucleic
GENBANK
Amino




Nucleotide
Acid
Amino Acid
Acid


GI
Sequence
SEQ
Sequence
SEQ
Source


Number
Accession Information
ID NO:
Accession No.
ID NO:
Organism















152964825
complement
1484
YP_001360609.1
1685

Kineococcus




(NC_009664.2:




radiotolerans




1430885 . . . 1432984)



SRS30216


88800302
complement
1485
ZP_01115869.1
1686

Reinekea




(NZ_AAOE01000024.1:




blandensis




59450 . . . 61609)



MED297


254786809
complement
1486
YP_003074238.1
1687

Teredinibacter




(NC_012997.1:




turnerae




3139764 . . . 3141917)



T7901


120554060
complement
1487
YP_958411.1
1688

Marinobacter




(NC_008740.1:




aquaeolei




1283732 . . . 1285885)



VT8


83647145
NC_007645.1:
1488
YP_435580.1
1689

Hahella




4579211 . . . 4581358




chejuensis








KCTC 2396


146308660
NC_009439.1:
1489
YP_001189125.1
1690

Pseudomonas




4021959 . . . 4024052




mendocina








ymp


116048757
NC_008463.1:
1490
YP_792443.1
1691

Pseudomonas




4740071 . . . 4742185




aeruginosa








UCBPP-PA14


28868382
complement
1491
NP_791001.1
1692

Pseudomonas




(NC_004578.1:




syringae pv.




1283902 . . . 1285992)



tomato str.







DC3000


70728320
complement
1492
YP_258069.1
1693

Pseudomonas




(NC_004129.6:




fluorescens




1081214 . . . 1083313)



Pf-5


104780139
complement
1493
YP_606637.1
1694

Pseudomonas




(NC_008027.1:




entomophila




952666 . . . 954756)



L48


226945506
complement
1494
YP_002800579.1
1695

Azotobacter




(NC_012560.1:




vinelandii




3530138 . . . 3532276)



DJ


146281510
complement
1495
YP_001171663.1
1696

Pseudomonas




(NC_009434.1:




stutzeri




1238536 . . . 1240632)



A1501


30248315
complement
1496
NP_840385.1
1697

Nitrosomonas




(NC_004757.1:




europaea




326321 . . . 328408)



ATCC 19718


226946148
NC_012560.1:
1497
YP_002801221.1
1698

Azotobacter




4145609 . . . 4147684




vinelandii








DJ


226357371
NC_012526.1:
1498
YP_002787111.1
1699

Deinococcus




2779899 . . . 2782016




deserti








VCD115


94984159
complement
1499
YP_603523.1
1700

Deinococcus




(NC_008025.1:




geothermalis




46701 . . . 48812)



DSM 11300


15805114
complement
1500
NP_293799.1
1701

Deinococcus




(NC_001263.1:




radiodurans




69707 . . . 71875)



R1


89899079
complement
1501
YP_521550.1
1702

Rhodoferax




(NC_007908.1:




ferrireducens




264127 . . . 266178)



T118


90422592
NC_007925.1:
1502
YP_530962.1
1703

Rhodopseudomonas




1181422 . . . 1183572




palustris








BisB18


90423512
NC_007925.1:
1503
YP_531882.1
1704

Rhodopseudomonas




2183340 . . . 2185475




palustris








BisB18


115525859
NC_008435.1:
1504
YP_782770.1
1705

Rhodopseudomonas




4320999 . . . 4323140




palustris








BisA53


167574473
complement
1505
ZP_02367347.1
1706

Burkholderia




(NZ_ABBG01000507.1:




oklahomensis




7891 . . . 9969)



C6786


83594327
complement
1506
YP_428079.1
1707

Rhodospirillum




(NC_007643.1:




rubrum




3449832 . . . 3451943)



ATCC 11170


90422165
NC_007925.1:
1507
YP_530535.1
1708

Rhodopseudomonas




696325 . . . 698388




palustris








BisB18


34496985
complement
1508
NP_901200.1
1709

Chromobacterium




(NC_005085.1:




violaceum




1636285 . . . 1638366)



ATCC 12472


224825239
complement
1509
ZP_03698345.1
1710

Lutiella




(NZ_ACIS01000004.1:




nitroferrum




398128 . . . 400215)



2002


148652157
complement
1510
YP_001279250.1
1711

Psychrobacter




(NC_009524.1:



sp. PRwf-1



415573 . . . 417720)


93005047
complement
1511
YP_579484.1
1712

Psychrobacter




(NC_007969.1:




cryohalolentis




257926 . . . 260082)



K5


257453691
NZ_ACYI01000010.1:
1512
ZP_05618978.1
1713

Enhydrobacter




16653 . . . 18797




aerosaccus








SK60


255321153
NZ_ACVR01000080.1:
1513
ZP_05362319.1
1714

Acinetobacter




44385 . . . 46529




radioresistens








SK82


50083778
complement
1514
YP_045288.1
1715

Acinetobacter




(NC_005966.1:



sp. ADP1



527524 . . . 529686)


260549093
NZ_GG704496.1:
1515
ZP_05823314.1
1716

Acinetobacter




86045 . . . 88189



sp. RUH2624


226953952
complement
1516
ZP_03824416.1
1717

Acinetobacter




(NZ_ABYN01000201.1:



sp. ATCC 27244



23157 . . . 25289)


153005955
NC_009675.1:
1517
YP_001380280.1
1718

Anaeromyxobacter




3624676 . . . 3626811



sp. Fw109-5


86159318
complement
1518
YP_466103.1
1719

Anaeromyxobacter




(NC_007760.1:




dehalogenans




3267950 . . . 3270094)



2CP-C


52425053
complement
1519
YP_088190.1
1720

Mannheimia




(NC_006300.1:




succiniciproducens




977458 . . . 979596)



MBEL55E


152979320
NC_009655.1:
1520
YP_001344949.1
1721

Actinobacillus




1823344 . . . 1825485




succinogenes








130Z


251792685
NC_012913.1:
1521
YP_003007411.1
1722

Aggregatibacter




968721 . . . 970856




aphrophilus








NJ8700


145633066
NZ_AAZF01000004.1:
1522
ZP_01788798.1
1723

Haemophilus




73469 . . . 75604




influenzae








3655


113460945
complement
1523
YP_719012.1
1724

Haemophilus




(NC_008309.1:




somnus




873911 . . . 876049)



129PT


15602570
NC_002663.1:
1524
NP_245642.1
1725

Pasteurella




821181 . . . 823319




multocida








subsp.








multocida








str. Pm70


260913970
complement
1525
ZP_05920444.1
1726

Pasteurella




(NZ_ACZR01000013.1:




dagmatis




172766 . . . 174904)



ATCC 43325


53729159
complement
1526
ZP_00133992.2
1727

Actinobacillus




(NZ_AACK01000004.1:




pleuropneumoniae




12180 . . . 14318)



serovar 1 str. 4074


240949203
NZ_ACQL01000097.1:
1527
ZP_04753547.1
1728

Actinobacillus




15931 . . . 18069




minor








NM305


33152520
NC_002940.2:
1528
NP_873873.1
1729

Haemophilus




1192390 . . . 1194528




ducreyi








35000HP


254362832
NZ_DS264681.1:
1529
ZP_04978908.1
1730

Mannheimia




4949 . . . 7084




haemolytica








PHL213


219870647
NC_011852.1:
1530
YP_002475022.1
1731

Haemophilus




435431 . . . 437566




parasuis








SH0165


258637834
NZ_ACYJ01000022.1:
1531
ZP_05730581.1
1732

Pantoea




41620 . . . 43764



sp. At-9b


188533336
complement
1532
YP_001907133.1
1733

Erwinia




(NC_010694.1:




tasmaniensis




1324250 . . . 1326379)



Et1/99


85059585
NC_007712.1:
1533
YP_455287.1
1734

Sodalis




2759501 . . . 2761645




glossinidius








str. ‘morsitans


258631105
complement
1534
ZP_05723922.1
1735

Dickeya




(NZ_ACYK01000004.1:




dadantii




104546 . . . 106687)



Ech586


261820783
complement
1535
YP_003258889.1
1736

Pectobacterium




(NC_013421.1:




wasabiae




1606509 . . . 1608647)



WPP163


242239978
NC_012880.1:
1536
YP_002988159.1
1737

Dickeya




3004583 . . . 3006724




dadantii








Ech703


22125515
complement
1537
NP_668938.1
1738

Yersinia




(NC_004088.1:




pestis




1788905 . . . 1791058)



KIM 10


157371554
NC_009832.1:
1538
YP_001479543.1
1739

Serratia




3673293 . . . 3675482




proteamaculans








568


238920583
NC_012779.1:
1539
YP_002934098.1
1740

Edwardsiella




2589897 . . . 2592035




ictaluri








93-146


197285630
NC_010554.1:
1540
YP_002151502.1
1741

Proteus




1898593 . . . 1900737




mirabilis








HI4320


37526984
NC_005126.1:
1541
NP_930328.1
1742

Photorhabdus




3612456 . . . 3614597




luminescens








subsp.








laumondii








TTO1


238895817
NC_012731.1:
1542
YP_002920553.1
1743

Klebsiella




3763302 . . . 3765449




pneumoniae








NTUH-K2044


146312483
NC_009436.1:
1543
YP_001177557.1
1744

Enterobacter




3080629 . . . 3082770



sp. 638


260598715
NC_013282.1:
1544
YP_003211286.1
1745

Cronobacter




3057676 . . . 3059814




turicensis



601935
D21123.1:
1545
BAA04663.1
1746

Escherichia




77 . . . 2218




coli



238898722
complement
1546
YP_002924403.1
1747

Candidatus




(NC_012751.1:




Hamiltonella




1494526 . . . 1496655)




defensa 5AT








(Acyrthosiphon








pisum)



227114079
NZ_ABVX01000029.1:
1547
ZP_03827735.1
1748

Pectobacterium




23117 . . . 25261




carotovorum








subsp.








brasiliensis








PBR1692


89072717
complement
1548
ZP_01159282.1
1749

Photobacterium




(NZ_AAOU01000004.1:



sp. SKA34



98074 . . . 100221)


54309953
NC_006370.1:
1549
YP_130973.1
1750

Photobacterium




3245262 . . . 3247418




profundum








SS9


262274670
NZ_ADAQ01000011.1:
1550
ZP_06052481.1
1751

Grimontia




496361 . . . 498520




hollisae








CIP 101886


260768101
complement
1551
ZP_05877035.1
1752

Vibrio




(NZ_ACZP01000013.1:




furnissii




239301 . . . 241427)



CIP 102972


260773044
NZ_ACZO01000006.1:
1552
ZP_05881960.1
1753

Vibrio




1066216 . . . 1068360




metschnikovii








CIP 69.14


163802859
complement
1553
ZP_02196748.1
1754

Vibrio




(NZ_ABGR01000013.1:



sp. AND4



61871 . . . 64036)


37680318
NC_005139.1:
1554
NP_934927.1
1755

Vibrio




2144915 . . . 2147059




vulnificus








YJ016


149188151
complement
1555
ZP_01866446.1
1756

Vibrio




(NZ_ABCH01000004.1:




shilonii




163878 . . . 166022)



AK1


218708991
complement
1556
YP_002416612.1
1757

Vibrio




(NC_011753.1:




splendidus




1031606 . . . 1033810)



LGP32


209695557
NC_011312.1:
1557
YP_002263486.1
1758

Aliivibrio




2262635 . . . 2264806




salmonicida








LFI1238


229525709
complement
1558
ZP_04415114.1
1759

Vibrio




(NZ_ACHV01000001.1:




cholerae bv.




2574339 . . . 2576483)




albensis








VL426


145300284
NC_009348.1:
1559
YP_001143125.1
1760

Aeromonas




3681431 . . . 3683584




salmonicida








subsp.








salmonicida








A449


237807651
complement
1560
YP_002892091.1
1761

Tolumonas




(NC_012691.1:




auensis




958413 . . . 960569)



DSM 9187


90407162
complement
1561
ZP_01215350.1
1762

Psychromonas




(NZ_AAPG01000006.1:



sp. CNPT3



41954 . . . 44116)


119946918
NC_008709.1:
1562
YP_944598.1
1763

Psychromonas




4084304 . . . 4086466




ingrahamii








37


157374843
complement
1563
YP_001473443.1
1764

Shewanella




(NC_009831.1:




sediminis




2041698 . . . 2043839)



HAW-EB3


170727231
NC_010506.1:
1564
YP_001761257.1
1765

Shewanella




3531467 . . . 3533608




woodyi








ATCC 51908


127513322
NC_009092.1:
1565
YP_001094519.1
1766

Shewanella




2807561 . . . 2809699




loihica








PV-4


167624517
NC_010334.1:
1566
YP_001674811.1
1767

Shewanella




3149368 . . . 3151515




halifaxensis








HAW-EB4


117919999
complement
1567
YP_869191.1
1768

Shewanella




(NC_008577.1:



sp. ANA-3



1806421 . . . 180857


119774631
complement
1568
YP_927371.1
1769

Shewanella




(NC_008700.1:




amazonensis




1807689 . . . 1809827)



SB2B


114563647
NC_008345.1:
1569
YP_751160.1
1770

Shewanella




2956515 . . . 2958662




frigidimarina








NCIMB 400


91793762
NC_007954.1:
1570
YP_563413.1
1771

Shewanella




2868611 . . . 2870791




denitrificans








OS217


157376672
NC_009831.1:
1571
YP_001475272.1
1772

Shewanella




4313346 . . . 4315484




sediminis








HAW-EB3


167624655
complement
1572
YP_001674949.1
1773

Shewanella




(NC_010334.1:




halifaxensis




3320048 . . . 3322198)



HAW-EB4


239996136
complement
1573
ZP_04716660.1
1774

Alteromonas




(NZ_ABQB01000564.1:




macleodii




6079 . . . 8301)



ATCC 27126


109898905
NC_008228.1:
1574
YP_662160.1
1775

Pseudoalteromonas




3144369 . . . 3146513




atlantica








T6c


119469286
NZ_AAVS01000006.1:
1575
ZP_01612225.1
1776

Alteromonadales




30053 . . . 32206




bacterium








TW-7


88860001
complement
1576
ZP_01134640.1
1777

Pseudoalteromonas




(NZ_AAOH01000005.1:




tunicata




230650 . . . 232797)



D2


71282469
NC_003910.7:
1577
YP_269873.1
1778

Colwellia




3309465 . . . 3311585




psychrerythraea








34H


152996332
NC_009654.1:
1578
YP_001341167.1
1779

Marinomonas




2608121 . . . 2610220



sp. MWYL1


87121463
NZ_AANE01000011.1:
1579
ZP_01077352.1
1780

Marinomonas




112021 . . . 114096



sp. MED121


146328905
NC_009446.1:
1580
YP_001209362.1
1781

Dichelobacter




489780 . . . 491837




nodosus








VCS1703A


258544959
NZ_ACKY01000059.1:
1581
ZP_05705193.1
1782

Cardiobacterium




4448 . . . 6562




hominis








ATCC 15826


262104765
complement
1582
EEY62817.1
1783

Phytophthora




(DS028152.1:




infestans




677306 . . . 679621)



T30-4


262104764
complement
1583
EEY62816.1
1784

Phytophthora




(DS028152.1:




infestans




674496 . . . 676781)



T30-4


159472743
XM_001694452.1:
1584
XP_001694504.1
1785

Chlamydomonas




258 . . . 2636




reinhardtii



168000833
XM_001753068.1:
1585
XP_001753120.1
1786

Physcomitrella




1 . . . 2367




patens








subsp.








Patens



172038009
complement
1586
YP_001804510.1
1787

Cyanothece




(NC_010546.1:



sp. ATCC 51142



3214848 . . . 3216944)


126658068
NZ_AAXW01000014.1:
1587
ZP_01729220.1
1788

Cyanothece




79066 . . . 81162



sp. CCY0110


257060449
NC_013161.1:
1588
YP_003138337.1
1789

Cyanothece




2659296 . . . 2661419



sp. PCC 8802


218441705
complement
1589
YP_002380034.1
1790

Cyanothece




(NC_011729.1:



sp. PCC 7424



5341705 . . . 5343810)


166368837
NC_010296.1:
1590
YP_001661110.1
1791

Microcystis




5646854 . . . 5648950




aeruginosa








NIES-843


220909840
NC_011884.1:
1591
YP_002485151.1
1792

Cyanothece




4551169 . . . 4553265



sp. PCC 7425


16330299
NC_000911.1:
1592
NP_441027.1
1793

Synechocystis




1250442 . . . 1252535



sp. PCC 6803


86142732
NZ_AANC01000005.1:
1593
ZP_01061171.1
1794

Leeuwenhoekiella




209172 . . . 211268




blandensis








MED217


146301271
complement
1594
YP_001195862.1
1795

Flavobacterium




(NC_009441.1:




johnsoniae




4208789 . . . 4210882)



UW101


260061847
NC_013222.1:
1595
YP_003194927.1
1796

Robiginitalea




1408358 . . . 1410454




biformata








HTCC2501


88713711
complement
1596
ZP_01107792.1
1797

Flavobacteriales




(NZ_AAOC01000008.1:




bacterium




22821 . . . 24917)



HTCC2170


86133149
complement
1597
ZP_01051731.1
1798

Polaribacter




(NZ_CH902588.1:



sp. MED152



146636 . . . 148729)


88803680
NZ_AAOG01000005.1:
1598
ZP_01119204.1
1799

Polaribacter




54861 . . . 56951




irgensii








23-P


213962668
NZ_ABZV01000006.1:
1599
ZP_03390929.1
1800

Capnocytophaga




103363 . . . 105438




sputigena








ATCC 33612


256820698
complement
1600
YP_003141977.1
1801

Capnocytophaga




(NC_013162.1:




ochracea




2243972 . . . 2246047)



DSM 7271


46581432
NC_002937.3:
1601
YP_012240.1
1802

Desulfovibrio




3152216 . . . 3154330




vulgaris str.








Hildenborough


218886955
NC_011769.1:
1602
YP_002436276.1
1803

Desulfovibrio




2286534 . . . 2288648




vulgaris str.








‘Miyazaki F’


78358281
NC_007519.1:
1603
YP_389730.1
1804

Desulfovibrio




3235663 . . . 3237822




desulfuricans








subsp.








desulfuricans








str. G20


242280036
complement
1604
YP_002992165.1
1805

Desulfovibrio




(NC_012881.1:




salexigens




2812652 . . . 2814769)



DSM 2638


258405159
complement
1605
YP_003197901.1
1806

Desulfohalobium




(NC_013223.1:




retbaense




1218708 . . . 1220816)



DSM 5692


256828849
NC_013173.1:
1606
YP_003157577.1
1807

Desulfomicrobium




1143375 . . . 1145477




baculatum








DSM 4028


225198782
complement
1607
ZP_03737911.1
1808

Desulfonatronospira




(NZ_ACJN01000010.1:




thiodismutans




60728 . . . 62824)



ASO3-1


242278203
NC_012881.1:
1608
YP_002990332.1
1809

Desulfovibrio




802309 . . . 804414




salexigens








DSM 2638


212704109
complement
1609
ZP_03312237.1
1810

Desulfovibrio




(NZ_ABXU01000065.1:




piger




34368 . . . 36470)



ATCC 29098


220903578
complement
1610
YP_002478890.1
1811

Desulfovibrio




(NC_011883.1:




desulfuricans




357004 . . . 359112)



subsp.








desulfuricans








str. ATCC 27774


51244410
NC_006138.1:
1611
YP_064294.1
1812

Desulfotalea




608983 . . . 611115




psychrophila








LSv54


94986723
NC_008011.1:
1612
YP_594656.1
1813

Lawsonia




347892 . . . 350012




intracellularis








PHE/MN1-00


119488858
complement
1613
ZP_01621820.1
1814

Lyngbya




(NZ_AAVU01000021.1:



sp. PCC 8106



33384 . . . 35414)


209524350
NZ_ABYK01000010.1:
1614
ZP_03272899.1
1815

Arthrospira




35808 . . . 37916




maxima








CS-328


116748909
NC_008554.1:
1615
YP_845596.1
1816

Syntrophobacter




1826816 . . . 1828915




fumaroxidans








MPOB


241776655
NZ_ACQQ01000008.1:
1616
ZP_04773932.1
1817

Allochromatium




86078 . . . 88198




vinosum








DSM 180


32476008
NC_005027.1:
1617
NP_869002.1
1818

Rhodopirellula




5198833 . . . 5200932




baltica








SH 1


78776256
NC_007575.1:
1618
YP_392571.1
1819

Sulfurimonas




60204 . . . 62282




denitrificans








DSM 1251


254458291
complement
1619
ZP_05071717.1
1820

Campylobacterales




(NZ_DS995288.1:




bacterium




173480 . . . 175561)



GD 1


229532518
NZ_ABUV01000006.1:
1620
ZP_04421899.1
1821

Sulfurospirillum




73569 . . . 75677




deleyianum








DSM 6946


152993574
NC_009663.1:
1621
YP_001359295.1
1822

Sulfurovum




2069625 . . . 2071724



sp. NBC37-1


120401715
NC_008726.1:
1622
YP_951544.1
1823

Mycobacterium




740616 . . . 742694




vanbaalenii








PYR-1


145220810
complement
1623
YP_001131488.1
1824

Mycobacterium




(NC_009338.1:




gilvum




189392 . . . 191515)



PYR-GCK


108797517
NC_008146.1:
1624
YP_637714.1
1825

Mycobacterium




594117 . . . 596231



sp. MCS


118473540
NC_008596.1:
1625
YP_885188.1
1826

Mycobacterium




867578 . . . 869656




smegmatis








str. MC2 155


169631304
complement
1626
YP_001704953.1
1827

Mycobacterium




(NC_010397.1:




abscessus




4294451 . . . 4296532)


240168870
NZ_ACBV01000011.1:
1627
ZP_04747529.1
1828

Mycobacterium




33884 . . . 35974




kansasii








ATCC 12478


183980733
NC_010612.1:
1628
YP_001849024.1
1829

Mycobacterium




853987 . . . 856071




marinum








M


15607549
NC_000962.2:
1629
NP_214922.1
1830

Mycobacterium




491786 . . . 493858




tuberculosis








H37Rv


41409983
NC_002944.2:
1630
NP_962819.1
1831

Mycobacterium




4345845 . . . 4347932




avium








subsp.








paratuberculosis








K-10


254818871
complement
1631
ZP_05223872.1
1832

Mycobacterium




(NZ_ABIN01000026.1:




intracellulare




2280 . . . 4439)



ATCC 13950


226304961
NC_012490.1:
1632
YP_002764919.1
1833

Rhodococcus




1605870 . . . 1607948




erythropolis








PR4


111019190
complement
1633
YP_702162.1
1834

Rhodococcus




(NC_008268.1:




jostii




2308925 . . . 2311045)



RHA1


54027320
complement
1634
YP_121562.1
1835

Nocardia




(NC_006361.1:




farcinica




5652808 . . . 5654895)



IFM 10152


227978095
NZ_ABVA01000001.1:
1635
ZP_04025361.1
1836

Tsukamurella




785441 . . . 787570




paurometabola








DSM 20162


262204223
complement
1636
YP_003275431.1
1837

Gordonia




(NC_013441.1:




bronchialis




4704088 . . . 4706208)



DSM 43247


256831883
complement
1637
YP_003160610.1
1838

Jonesia




(NC_013174.1:




denitrificans




683848 . . . 685947)



DSM 20603


260517199
complement
1638
ZP_05816650.1
1839

Sanguibacter




(NZ_ABUN01000002.1:




keddieii




90744 . . . 92939)



DSM 10542


229243856
complement
1639
ZP_04368027.1
1840

Cellulomonas




(NZ_ABTJ01000131.1:




flavigena




4381 . . . 6468)



DSM 20109


229821528
NC_012669.1:
1640
YP_002883054.1
1841

Beutenbergia




3401218 . . . 3403323




cavernae








DSM 12333


227428424
complement
1641
ZP_03911481.1
1842

Xylanimonas




(NZ_ABVC01000008.1:




cellulosilytica




150308 . . . 152407)



DSM 15894


119717178
complement
1642
YP_924143.1
1843

Nocardioides




(NC_008699.1:



sp. JS614



3139954 . . . 3142044)


227381337
complement
1643
ZP_03864789.1
1844

Kribbella




(NZ_ABUC01000011.1:




flavida




233655 . . . 235784)



DSM 17836


88856399
NZ_AAOB01000010.1:
1644
ZP_01131057.1
1845
marine



2970 . . . 5138




actinobacterium








PHSC20C1


170780609
NC_010407.1:
1645
YP_001708941.1
1846

Clavibacter




179995 . . . 182112




michiganensis








subsp.








Sepedonicus



50954174
NC_006087.1:
1646
YP_061462.1
1847

Leifsonia




335128 . . . 337257




xyli








subsp.








xyli








str. CTCB07


114331961
complement
1647
YP_748183.1
1848

Nitrosomonas




(NC_008344.1:




eutropha




2107910 . . . 2110039)



C91


256395328
complement
1648
YP_003116892.1
1849

Catenulispora




(NC_013131.1:




acidiphila




7130890 . . . 7133034)



DSM 44928


258650827
NC_013235.1:
1649
YP_003199983.1
1850

Nakamurella




639398 . . . 641491




multipartita








DSM 44233


257068066
complement
1650
YP_003154321.1
1851

Brachybacterium




(NC_013172.1:




faecium




995592 . . . 997667)



DSM 4810


227497260
complement
1651
ZP_03927492.1
1852

Actinomyces




(NZ_ACFH01000109.1:




urogenitalis




3054 . . . 5108)



DSM 15434


256824971
NC_013169.1:
1652
YP_003148931.1
1853

Kytococcus




1149994 . . . 1152081




sedentarius








DSM 20547


260455562
NZ_ACZH01000022.1:
1653
ZP_05803950.1
1854

Streptomyces




9845 . . . 11917




flavogriseus








ATCC 33331


182435904
NC_010572.1:
1654
YP_001823623.1
1855

Streptomyces




2506931 . . . 2509012




griseus








subsp.








griseus








NBRC 13350


254387454
NZ_DS570624.1:
1655
ZP_05002693.1
1856

Streptomyces




26512 . . . 28596




clavuligerus








ATCC 27064


254400535
NZ_DS570905.1:
1656
ZP_05015493.1
1857

Streptomyces




178100 . . . 180199




sviceus








ATCC 29083


256813645
NZ_ACFA01000303.1:
1657
ZP_05538660.1
1858

Streptomyces




9482 . . . 11584




griseoflavus








Tu4000


239928836
NZ_ABYA01000185.1:
1658
ZP_04685789.1
1859

Streptomyces




2180 . . . 4282




ghanaensis








ATCC 14672


256804684
complement
1659
ZP_05534308.1
1860

Streptomyces




(NZ_ACEZ01000169.1:




viridochromogenes




8822 . . . 10924)



DSM 40736


256785123
NZ_ACEY01000098.1:
1660
ZP_05523554.1
1861

Streptomyces




97187 . . . 99280




lividans








TK24


29829365
NC_003155.4:
1661
NP_823999.1
1862

Streptomyces




3467325 . . . 3469415




avermitilis








MA-4680


260646824
FN554889.1:
1662
CBG69921.1
1863

Streptomyces




3224239 . . . 3226344




scabiei








87.22


239982381
complement
1663
ZP_04704905.1
1864

Streptomyces




(NZ_ABYC01000362.1:




albus




40088 . . . 42163)



J1074


254382385
complement
1664
ZP_04997745.1
1865

Streptomyces




(NZ_DS570390.1:



sp. Mg1



111950 . . . 114034)


256769973
complement
1665
ZP_05509147.1
1866

Streptomyces




(NZ_ACEW01000403.1:



sp. C



16454 . . . 18541)


256776255
NZ_ACEX01000277.1:
1666
ZP_05514718.1
1867

Streptomyces




2545 . . . 4641




hygroscopicus








ATCC 53653


254378850
NZ_DS570550.1:
1667
ZP_04994290.1
1868

Streptomyces




40417 . . . 42507



sp. SPB74


229854086
complement
1668
ZP_04474082.1
1869

Streptosporangium




(NZ_ABUU01000066.1:




roseum




39364 . . . 41415)



DSM 43021


145596204
NC_009380.1:
1669
YP_001160501.1
1870

Salinispora




4234932 . . . 4237007




tropica








CNB-440


159039600
NC_009953.1:
1670
YP_001538853.1
1871

Salinispora




4631907 . . . 4633976




arenicola








CNS-205


238060866
NZ_GG657738.1:
1671
ZP_04605575.1
1872

Micromonospora




2330097 . . . 2332163



sp. ATCC 39149


116671783
complement
1672
YP_832716.1
1873

Arthrobacter




(NC_008541.1:



sp. FB24



3648900 . . . 3651011)


148807608
complement
1673
ABR13603.1
1874

Arthrobacter




(EF6018801:




oxydans




72 . . . 2150)


239916738
complement
1674
YP_002956296.1
1875

Micrococcus




(NC_012803.1:




luteus




190981 . . . 193056)



NCTC 2665


255326162
NZ_ACVO01000004.1:
1675
ZP_05367249.1
1876

Rothia




127571 . . . 129661




mucilaginosa








ATCC 25296


184199797
complement
1676
YP_001854004.1
1877

Kocuria




(NC_010617.1:




rhizophila




164688 . . . 166781)



DC2201


254368446
NZ_DS264133.1:
1677
ZP_04984463.1
1878

Francisella




69966 . . . 72062




tularensis








subsp.








holarctica








FSC022


167626922
complement
1678
YP_001677422.1
1879

Francisella




(NC_010336.1:




philomiragia




741506 . . . 743602)



subsp.








philomiragia








ATCC 25017


94676965
NC_007984.1:
1679
YP_588827.1
1880

Baumannia




392877 . . . 395012




cicadellinicola








str. Hc







(Homalodisca








coagulata)



P57273
BA000003.2:
1680
NP_240007.1
1881

Buchnera




189582 . . . 191708




aphidicola








str. APS







(Acyrthosiphon








pisum)



254444018
NZ_DS990592.1:
1681
ZP_05057494.1
1882

Verrucomicrobiae




1298899 . . . 1201010




bacterium








DG1235


171914782
NZ_ABIZ01000001.1:
1682
ZP_02930252.1
1883

Verrucomicrobium




6593044 . . . 6595128




spinosum








DSM4136


114777389
NZ_AATS01000006.1:
1683
ZP_01452386.1
1884

Mariprofundus




50467 . . . 52602




ferrooxydans








PV-1


94500866
NZ_AAQH01000011.1:
1684
ZP_01307392.1
1885

Bermanella




33324 . . . 35456




marisrubri










In other embodiments, a polynucleotide, gene and/or polypeptide encoding phosphotransacetylase can have at least about 70% to about 75%, about 80% to about 85%, about 85% to about 90%, about 90% to about 95%, about 96%, about 97%, about 98%, or about 99% sequence identity to any one of the sequences of Tables 10 or 12a or 12b, wherein the polynucleotide, gene and/or polypeptide encodes a polypeptide having phosphotransacetylase activity.


In embodiments, a polynucleotide, gene and/or polypeptide encoding phosphotransacetylase corresponds to the Enzyme Commission Number EC 2.3.1.8.


In other embodiments, the phosphotransacetylase polynucleotide, gene and/or polypeptide sequences described herein or those recited in the art can be used to identify phosphotransacetylase sequences or phosphotransacetylase homologs in other cells, as described above for PDC.


Methods for gene expression in recombinant host cells, including, but not limited to, yeast cells are known in the art (see, for example, Methods in Enzymology, Volume 194, Guide to Yeast Genetics and Molecular and Cell Biology (Part A, 2004, Christine Guthrie and Gerald R. Fink (Eds.), Elsevier Academic Press, San Diego, Calif.). In embodiments, the coding region for the phosphoketolase and/or phosphotransacetylase genes to be expressed can be codon optimized for the target host cell, as well known to one skilled in the art. Expression of genes in recombinant host cells, including but not limited to yeast cells, can require a promoter operably linked to a coding region of interest, and a transcriptional terminator. A number of promoters can be used in constructing expression cassettes for genes, including, but not limited to, the following constitutive promoters suitable for use in yeast: FBA1, TDH3 (GPD), ADH1, and GPM1; and the following inducible promoters suitable for use in yeast: GAL1, GAL10 and CUP1. Other yeast promoters include hybrid promoters UAS(PGK1)-FBA1p (SEQ ID NO: 1893), UAS(PGK1)-ENO2p (SEQ ID NO: 1894), UAS(FBA1)-PDC1p (SEQ ID NO: 1895), UAS(PGK1)-PDC1p (SEQ II) NO: 1896), and UAS(PGK)-OLE1p (SEQ ID NO: 1897). Suitable transcriptional terminators that can be used in a chimeric gene construct for expression include, but are not limited to, FBA1t, TDH3t, GPM1t. ERG10t, GAL1t, CYC1t, and ADH1t.


Recombinant polynucleotides are typically cloned for expression using the coding sequence as part of a chimeric gene used for transformation, which includes a promoter operably linked to the coding sequence as well as a ribosome binding site and a termination control region. The coding region may be from the host cell for transformation and combined with regulatory sequences that are not native to the natural gene encoding phosphoketolase and/or phosphotransacetylase. Alternatively, the coding region may be from another host cell.


Vectors useful for the transformation of a variety of host cells are common and described in the literature. Typically the vector contains a selectable marker and sequences allowing autonomous replication or chromosomal integration in the desired host. In addition, suitable vectors can comprise a promoter region which harbors transcriptional initiation controls and a transcriptional termination control region, between which a coding region DNA fragment may be inserted, to provide expression of the inserted coding region. Both control regions can be derived from genes homologous to the transformed host cell, although it is to be understood that such control regions can also be derived from genes that are not native to the specific species chosen as a production host.


In embodiments, suitable promoters, transcriptional terminators, and phosphoketolase and/or phosphotransacetylase coding regions can be cloned into E. coli-yeast shuttle vectors, and transformed into yeast cells. Such vectors allow strain propagation in both E. coli and yeast strains, and can contain a selectable marker and sequences allowing autonomous replication or chromosomal integration in the desired host. Typically used plasmids in yeast include, but are not limited to, shuttle vectors pRS423, pRS424, pRS425, and pRS426 (American Type Culture Collection, Rockville, Md.), which contain an E. coli replication origin (e.g., pMB1), a yeast 2-micron origin of replication, and a marker for nutritional selection. The selection markers for these four vectors are 141S3 (vector pRS423), TRP1 (vector pRS424), LEU2 (vector pRS425) and URA3 (vector pRS426).


In embodiments, construction of expression vectors with a chimeric gene encoding the described phosphoketolases and/or phosphotransacetylases can be performed by the gap repair recombination method in yeast. In embodiments, a yeast vector DNA is digested (e.g., in its multiple cloning site) to create a “gap” in its sequence. A number of insert DNAs of interest are generated that contain an approximately 21 by sequence at both the 5′ and the 3′ ends that sequentially overlap with each other, and with the 5′ and 3′ terminus of the vector DNA. For example, to construct a yeast expression vector for “Gene X,” a yeast promoter and a yeast terminator are selected for the expression cassette. The promoter and terminator are amplified from the yeast genomic DNA, and Gene X is either PCR amplified from its source organism or obtained from a cloning vector comprising Gene X sequence. There is at least a 21 by overlapping sequence between the 5′ end of the linearized vector and the promoter sequence, between the promoter and Gene X, between Gene X and the terminator sequence, and between the terminator and the 3′ end of the linearized vector. The “gapped” vector and the insert DNAs are then co-transformed into a yeast strain and plated on the medium containing the appropriate compound mixtures that allow complementation of the nutritional selection markers on the plasmids. The presence of correct insert combinations can be confirmed by PCR mapping using plasmid DNA prepared from the selected cells. The plasmid DNA isolated from yeast (usually low in concentration) can then be transformed into an E. coli strain, e.g. TOP10, followed by mini preps and restriction mapping to further verify the plasmid construct. Finally the construct can be verified by sequence analysis.


Like the gap repair technique, integration into the yeast genome also takes advantage of the homologous recombination system in yeast. In embodiments, a cassette containing a coding region plus control elements (promoter and terminator) and auxotrophic marker is PCR-amplified with a high-fidelity DNA polymerase using primers that hybridize to the cassette and contain 40-70 base pairs of sequence homology to the regions 5′ and 3′ of the genomic area where insertion is desired. The FCR product is then transformed into yeast and plated on medium containing the appropriate compound mixtures that allow selection for the integrated auxotrophic marker. For example, to integrate “Gene X” into chromosomal location “Y”, the promoter-coding region X-terminator construct is PCR amplified from a plasmid DNA construct and joined to an, autotrophic marker (such as URA3) by either SOE PCR or by common restriction digests and cloning. The full cassette, containing the promoter-coding regionX-terminator-URA3 region, is PCR amplified with primer sequences that contain 40-70 by of homology to the legions 5′ and 3′ of location “Y” on the yeast chromosome. The PCR product is transformed into yeast and selected on growth media lacking, uracil. Transformants can be verified either by colony PCR or by direct sequencing of chromosomal DNA.


The presence of phosphoketolase and phosphotransacetylase activity in the recombinant host cells disclosed herein can be, confirmed using routine methods known in the art. In a non-limiting example, and as described in the Examples herein, transformants can be screened by PCR using primers for the phosphoketolase and phosphotransacetylase genes. In embodiments, and as described in the Examples herein, transformants can be screened by PCR with primers N1039 and N1040 (SEQ ID NOs: 639 and 640) to confirm integration of the xpk1 gene, and primers N1041 and N1042 (SEQ ID NOs: 641 and 642) can be used to confirm integration of the eutD gene. In another non-limiting example, and as described in the Examples herein, transformants can be, screened for integration of phosphoketolase constructs and/or phosphotransacetylase constructs at the Δpdc1::ilvD(Sm) locus by the loss of ilvD(Sm) in the host cells.


In another non-limiting example, and as described in the examples herein, phosphoketolase activity can be assayed by expressing phosphoketolase identifiable by the methods disclosed herein in a recombinant host cell disclosed herein that lacks endogenous phosphoketolase activity. If phosphoketolase activity is present, such cells exhibit a reduced or eliminated requirement for exogenous two-carbon substrate supplementation for growth in culture.


In another non-limiting example, and as described in the examples herein, phosphoketolase and phosphotransacetylase activity can be assayed by expressing phosphoketolase and phosphotransacetylase activity identifiable by the methods disclosed herein in a recombinant host cell disclosed herein that lacks endogenous phosphoketolase and phosphotransacetylase activity. If phosphoketolase activity and phosphoketolase activity are present, such cells exhibit a reduced or eliminated requirement for exogenous two-carbon substrate supplementation for growth in culture.


In another non-limiting example, phosphoketolase, and/or phosphotransacetylase activity can be confirmed by more indirect methods, such as by assaying for a downstream product in a pathway requiring phosphoketolase activity. For example, a polypeptide having phosphoketolase activity can catalyze the conversion of xylulose-5-phosphate into glyceraldehyde-3-phosphate and acetyl-phosphate and/or the conversion of fructose-6-phosphate into erythrose-4-phosphate and acetyl-phosphate. Also, a polypeptide having phosphotransacetylase activity can catalyze the conversion of acetyl-phosphate into acetyl-CoA.


Suitable Pathway Carbon Substrates and Exogenous Two-Carbon Substrate Supplementation

PDC-KO cells fail to grow in glucose-containing media (e.g., 2% glucose), but PDC-KO cells carrying a functional butanediol biosynthetic pathway have been shown to grow on glucose supplemented with exogenous two-carbon substrates such as ethanol (see for example, US Patent Application Publication No. 20090305363, herein incorporated by reference). In embodiments, the host cells disclosed herein can be grown in fermentation media which contains a suitable pathway carbon substrate and two-substrate supplement, including combinations of suitable pathway carbon substrates with C2-substrate supplement. Non-limiting examples of suitable pathway carbon substrates include, but are not limited to, monosaccharides such as fructose, oligosaccharides such as lactose maltose, galactose, or sucrose, polysaccharides such as starch or cellulose or mixtures thereof and unpurified mixtures from renewable feedstocks such as cheese whey permeate, cornsteep liquor, sugar beet molasses, and barley malt, including any combinations thereof. In other embodiments, the suitable pathway carbon substrates can include lactate, glycerol, or combinations thereof.


In embodiments, a suitable carbon substrate can be a one-carbon substrate such as carbon dioxide, or methanol for which metabolic conversion into key biochemical intermediates has been demonstrated, or combinations thereof. In other embodiments related to methylotrophic organisms, the carbon substrate can be carbon containing compounds such as methylamine, glucosamine and a variety of amino acids for metabolic activity. In a non-limiting example, methylotrophic yeasts are known to utilize the carbon from methylamine to form trehalose or glycerol (Bellion et al., Microb. Growth C1 Compd., [Int. Symp.], 7th (1993), 415-32, Editor(s): Murrell, J. Collin; Kelly Don P. Publisher: Intercept, Andover, UK). In another non-limiting example, various species of Candida can metabolize alanine (Sulter et al., Arch. Microbiol. 153:485-489 (1990)). Hence it is contemplated that the source of carbon utilized in the present invention can encompass a wide variety of carbon containing substrates and will only be limited by the choice of organism.


In other embodiments. the suitable pathway carbon substrate can be glucose, fructose. and sucrose, or mixtures of these with five-ca hon (C5) sugars such as xylose and/or arabinose for yeasts cells modified to use C5 sugars. In embodiments. sucrose can be derived from renewable sugar sources such as sugar cane, sugar beets, cassaya; sweet sorghum and mixtures thereof. In other embodiment. glucose and dextrose can derived from renewable grain sources through saccharification of starch based feedstocks including grains such as corn, wheat, rye, barley, oats. and mixtures thereof. In embodiments, the pathway carbon substrates can be derived from renewable cellulosic or lignocellulosic biomass through processes of pretreatment and saccharification, as described, for example, in U.S. Patent Application Publication No. US 20070031918 A1, which is herein incorporated by reference.


As used herein, “biomass” refers to any cellulosic or lignocellulosic material and includes, but is not limited to, materials comprising cellulose, and optionally further comprising hemicellulose, lignin, starch, oligosaccharides and/or monosaccharides. In embodiments, biomass can also comprise additional components, such as protein and/or lipid. In other embodiments, biomass can be derived from a single source, or biomass can comprise a mixture derived from more than one source; for example, biomass can comprise a mixture of corn cobs and corn stover, or a mixture of grass and leaves. Biomass includes, but is not limited to, bioenergy crops, agricultural residues, municipal solid waste, industrial solid waste, sludge from paper manufacture, yard waste, wood and forestry waste. Other non-limiting examples of biomass include, but are not limited to, corn grain, corn cobs, crop residues such as corn husks, corn stover, grasses, wheat, wheat straw, barley, barley straw. hay, rice straw, switchgrass, waste paper, sugar cane bagasse. sorghum, soy, components obtained from milling of grains, trees, branches, roots, leaves, wood chips, sawdust, shrubs and bushes, vegetables, fruits, flowers, animal manure and mixtures thereof.


The recombinant host cells described herein can be cultured using standard laboratory techniques known in the art (see, e.g. Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring harbor, NY, pp. 201-202). In embodiments related to media supplemented with exogenous two-carbon substrates, and as described in, the Examples, recombinant host cells can be grown in synthetic complete medium supplemented with one or more exogenous two-carbon substrates as described herein at a concentration of about 0.01%, about 0.05%, about 0.1%, about 0.5%, about 1.0%, about 1.5%, about 1.5% or about 2% (v/v) of the media. In embodiments, the recombinant host cells can be grown in synthetic complete culture without uracil or histidine, supplemented with 0.5% (v/v) ethanol. In embodiments related to growth in media that is not supplemented with exogenous two-carbon substrates, the recombinant host cells described herein can be first grown in culture medium comprising an exogenous two carbon substrate and then diluted (e.g., starting OD=0.1! ml medium in a 125 ml vented flask) into media that is not supplemented with exogenous two-carbon substrate.


The growth of the recombinant, host cells described herein can be measured by methods known in the art (see, e.g., Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202). In a non-limiting example, the growth of the recombinant host cells described herein can be determined by measuring the optical density (01)) of cell cultures over time. For example, the OD at 600 nm for a yeast culture is proportional to yeast cell number. In another non-limiting example, the growth of the recombinant host cells described herein can be determined by counting viable cells in a sample of the culture over time.


Applicants have provided cells that have a reduced or eliminated requirement for two-carbon substrate supplementation for growth. In embodiments, such cells comprise (i) a deletion, mutation, and/or substitution in an endogenous gene encoding, a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA that results in a requirement for exogenous two-carbon substrate supplementation for optimal growth; (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity; and optionally (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. In embodiments, such cells comprise (i) a modification in an endogenous polypeptide having PDC activity which results in reduced or eliminated PDC activity; (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity; and optionally (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. As such, Applicants have also provided methods of improving the growth of a recombinant host cell comprising at least one modification in an endogenous polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA that results in a requirement for exogenous two-carbon substrate supplementation for optimal growth comprising transforming the host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. Applicants have also provided methods of improving the growth of a recombinant host cell comprising at least one, modification in an endogenous polypeptide having pyruvate decarboxylase activity (e.g., having at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having PDC activity that results in reduced or eliminated PDC activity) comprising transforming the host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. In other embodiments, the method further comprises transforming, a recombinant host cell described herein with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


Applicants have also provided methods of reducing or eliminating the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one modification in an endogenous activity that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA that results in a requirement for exogenous two-carbon substrate supplementation for optimal growth comprising transforming the host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity comprising, transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. In other embodiments, the method further comprises transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


Applicants have also provided methods of reducing the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one modification in an endogenous polypeptide having. PDC activity (e.g., having at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity) comprising transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. In other embodiments, the method further comprises transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


In addition, Applicants have provided methods of eliminating the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one modification in an endogenous polypeptide having PDC activity (e.g., having at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having PDC activity that results in reduced or eliminated PDC activity) comprising transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. In other embodiments, the method further comprises transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


In embodiments, a reduced requirement for exogenous two-carbon substrate supplementation can be a growth rate of the recombinant host cells described herein in media that is not supplemented, with an exogenous two-carbon substrate that is the same or substantially equivalent to the growth rate of a recombinant host cell comprising a modification in an endogenous activity that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA grown in media that is supplemented with an exogenous two-carbon substrate. In embodiments, such a growth rate can be at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% of the growth rate of a recombinant host cell comprising a modification in an endogenous activity that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA grown in media that is supplemented with an exogenous two-carbon substrate.


In embodiments, a reduced requirement for exogenous two-carbon substrate supplementation can be a growth rate of the recombinant host cells described herein in media that is not supplemented with an exogenous two-carbon substrate that is the same or substantially equivalent to the growth rate of a recombinant host cell comprising a modification in an endogenous PDC activity grown in media that is supplemented with an exogenous two-carbon substrate. In embodiments, such a growth rate can be at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% of the growth rate of a recombinant host cell comprising a modification in an endogenous PDC activity grown in media that is supplemented with an exogenous two-carbon substrate.


In other embodiments, the recombinant host cells described herein have a growth rate in media that is not supplemented with an exogenous two-carbon substrate that is greater than the growth rate of a recombinant host cell comprising a modification in an endogenous activity that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA in media that is not supplemented with an exogenous two-carbon substrate.


In other embodiments, the recombinant host cells described herein have a growth rate in media that is not supplemented with an exogenous two-carbon substrate that is greater than the growth rate of a recombinant host cell comprising a modification in an endogenous PDC activity in media that is not supplemented with an exogenous two-carbon substrate.


In other embodiments, the recombinant host cells described herein can have an increased glucose consumption compared to a recombinant host cell comprising a modification in an endogenous polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA.


In other embodiments, the recombinant host cells described herein can have an increased glucose consumption compared to a recombinant host cell comprising a modification in an endogenous polypeptide having PDC activity (e.g., at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide having PDC activity that reduces or eliminates PDC activity).


Glucose consumption of the recombinant host cells described herein can be measured by methods known in the art (see, e.g., Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202). In a non-limiting example, glucose consumption can be measured by quantitating the amount of glucose in culture media by HPLC or with a YSI Biochemistry Analyzer (YSI, Inc., Yellow Springs, Ohio).


In other embodiments, methods of producing a recombinant host cell are provided comprising transforming a recombinant host cell comprising a modification in an endogenous polynucleotide, gene or polypeptide encoding pyruvate decarboxylase (e.g., at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity) with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. In other embodiments, the method further comprises transforming the recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.


In other embodiments, methods for the conversion of xylulose 5-phosphate or fructose 6-phosphate into acetyl-phosphate are provided comprising (i) providing a recombinant host cell as described herein, or combinations thereof; and (ii) growing the recombinant host cell under conditions wherein xylulose 5-phosphate or fructose-6-phosphate is converted into acetyl-phosphate. In other embodiments, methods for the conversion of xylulose 5-phosphate or fructose-6-phosphate into acetyl-CoA are provided comprising (i) providing a recombinant rose cell as described herein, or combinations thereof; and (ii) growing the recombinant host cell under conditions where xylulose 5-phosphate or fructose-6-phosphate is converted into acetyl-CoA.


In other embodiments, methods for the conversion of acetyl-phosphate to, acetyl-CoA are provided comprising (i) providing a recombinant host cell as described herein, or combinations thereof; and (ii) growing the recombinant host cell under conditions where acetyl-phosphate is converted into acetyl-CoA. In other embodiments, methods for increasing the specific activity of a heterologous polypeptide having phosphoketolase activity in a recombinant host cell are provided comprising (i) providing a recombinant host cell as described herein, or combinations thereof; and (ii) growing the recombinant host cell under conditions wherein the heterologous polypeptide having phosphoketolase activity is expressed in functional form having a specific activity greater than the same recombinant host cell lacking the heterologous polypeptide having phosphoketolase activity.


In other embodiments, methods for increasing the specific activity of a heterologous polypeptide having phosphotransacetylase activity in a recombinant host cell are provided comprising (i) providing a recombinant host cell described herein, or combinations thereof; and (ii) growing the recombinant host cell under conditions whereby the heterologous polypeptide having phosphotransacetylase activity is expressed in functional form having a specific activity greater than the same recombinant host cell lacking a heterologous polypeptide having phosphotransacetylase activity.


In still other embodiments, methods for increasing the activity of the phosphoketolase pathway in a recombinant host cell are provided comprising (i) providing a recombinant host cell as described herein, or combinations thereof; and (ii) growing the host cell under conditions whereby the activity of the phosphoketolase pathway in the host cell is increased.


Threonine aldolase (E.C. number 4.1.2.5) catalyzes cleavage of threonine to produce glycine and acetaldehyde. Plasmid-based overexpression of a gene encoding this enzyme in S. cerevisiae PDC-KO strains was shown to eliminate the requirement for exogenous C2 supplementation (van Maris et al, Appl Environ Microbiol. 2003 April; 69(4):2094-9). In embodiments, recombinant host cells comprise (i) a deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA that results in a requirement for exogenous two-carbon substrate supplementation for optimal growth; and (ii) heterologous polynucleotide encoding a polypeptide having threonine aldolase activity.


Engineered Biosynthetic Pathways Using Pyruvate

In embodiments, the recombinant host cells described herein can be engineered to have a biosynthetic pathway for production of a product from pyruvate. A product from such a pyruvate-utilizing biosynthetic pathway includes, but is not limited to, 2,3-butanediol, isobutanol, 2-butanol, 2-butanone, valine, leucine, alanine, lactic acid, malic acid, fumaric acid, succinic acid and isoamyl alcohol. The features of any pyruvate-utilizing biosynthetic pathway may be engineered in the recombinant host cells described herein in any order. Any product made using a biosynthetic pathway that has, pyruvate as the initial substrate can be produced with greater effectiveness in a recombinant host cell disclosed herein having a, modification in an endogenous polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA (such as pyruvate decarboxylase, pyruvate formate lyase, pyruvate dehydrogenase, pyruvate oxidase, or pyruvate:ferredoxin oxioreductase) and having heterologous phosphoketolase and/or phosphotransacetylase activity, compared to a recombinant host cell having a modification in an endogenous polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA (such as pyruvate decarboxylase, pyruvate formate lyase, pyruvate dehydrogenase, pyruvate oxidase, or pyruvate:ferredoxin oxioreductase). Any product made using a biosynthetic pathway that has pyruvate as the initial substrate can be produced with greater effectiveness in a recombinant host cell disclosed herein having a modification in an endogenous polypeptide having PDC activity that reduces or eliminates PDC activity and having heterologous phosphoketolase and/or phosphotransacetylase activity, compared to a recombinant host cell having a modification in an endogenous polypeptide having PDC activity that reduces or eliminates PDC activity.


The biosynthetic pathway of the recombinant host cells described herein can be any pathway that utilizes pyruvate and produces a desired product. The pathway genes may include endogenous genes and/or heterologous genes. Typically at least one gene in the biosynthetic pathway is a heterologous gene. Suitable biosynthetic pathways for production of butanol are known in the art, and certain suitable pathways are described herein. In some embodiments, the butanol biosynthetic pathway comprises, at least one gene that is heterologous to the host cell. In some embodiments, the butanol biosynthetic pathway comprises more than one gene that is heterologous to the host cell. In some embodiments, the butanol biosynthetic pathway comprises heterologous genes encoding polypeptides corresponding to every step of a biosynthetic pathway.


Genes and polypeptides that can be used for substrate to product conversions described herein as well as methods of identifying such, genes and polypeptides, are described herein and/or in the art, for example, for isobutanol, in the Examples and in U.S. Pat. No. 7,851,188. Ketol-acid reductoisomerase (KAKI) enzymes are described in U.S. Patent Appl. Pub. Nos. 20080261230 A1, 20090163376 A1, 20100197519 A1, and PCT Appl. Pub. No. WO/2011/04.1415. Examples of KARIs disclosed therein are those from Lactococcus lactis, Vibrio cholera, Pseudomonas aeruginosa PAO1, and Pseudomonas fluorescens PF5 mutants. KARIs include Anaerostipes caccae KAR1 variants “K9G9” and “K9D3” (SEQ ID NOs: 1911 and 1910, respectively). US Appl. Pub. No. 20100081154 A1, and U.S. Pat. No. 7,851,188 describe dihydroxyacid dehydratases (DHADs), including a DHAD from Streptococcus mutans. U.S. Patent Appl. Publ. No. 20090269823 A1 describes SadB, an alcohol dehydrogenase (ADH) from Achromobacter xylosoxidans. Alcohol dehydrogenases also include horse liver ADH and Beijerinkia indica ADH (protein SEQ ID NO: 1923).


An example of a biosynthetic pathway for producing 2,3-butanediol can be engineered in the recombinant host cells described herein, as described in U.S. Patent Application No. 20090305363, which is herein incorporated by reference. The 2,3-butanediol pathway is a portion of the 2-butanol biosynthetic pathway that is disclosed in U.S. Patent Application Publication No. US 20070292927 A1, which is herein incorporated by reference. Such pathway steps include, but are not limited to, conversion of pyruvate to acetolactate by acetolactate synthase, conversion of acetolactate to acetoin by acetolactate decarboxylase, and conversion of acetoin to 2,3-butanediol by butanediol dehydrogenase. The skilled person will appreciate that polypeptides having the activity of such pathway steps can be isolated from a variety of sources can be used in the recombinant host cells described herein.


In addition, examples of biosynthetic pathways for production of 2-butanone or 2-butanol that can be engineered in the recombinant host cells described herein are disclosed in U.S. Patent Application Publication Nos. US 20070292927 A1 and US 20070259410 A1, which are herein incorporated by reference. The pathway in U.S. Patent Application Publication No. US 20070292927 A1 is the same as described for butanediol production with the addition of the following steps:


2,3-butanediol to 2-butanone as catalyzed for example by diol dehydratase or glycerol dehydratase; and


2-butanone to 2-butanol as catalyzed for example by butanol dehydrogenase.


Described in U.S. Patent Application Publication No. US 20090155870 A1, which is herein incorporated by reference, is the construction of chimeric genes and genetic engineering of yeast for 2-butanol production using the U.S. Patent Application Publication No. US 20070292927 A1 disclosed biosynthetic pathway. Further description for gene construction and expression related to these pathways can be found, for example, in International Publication No. WO 2009046370 (e.g., butanediol dehydratases); and U.S. Patent Application Publication No. US 20090269823 A1 (e.g., butanol dehydrogenase) and U.S. Patent Application Publication No. US 20070259410 A1 which are herein incorporated by reference. The skilled person will appreciate that polypeptides having the activity of such pathway steps can be isolated from a variety of sources can be used in the recombinant host cells described herein.


Biosynthetic pathways for the production of isobutanol that may be used include those described in U.S. Pat. No. 7,851,188 and PCT Publication WO 2007050671, incorporated herein by reference. One isobutanol biosynthetic pathway comprises the following substrate to product conversions:


pyruvate to acetolactate, which may be catalyzed, for example, by acetolactate synthase;


acetolactate to 2,3-dihydroxyisovalerate, which may be catalyzed, for example, by acetohydroxy acid reductoisomerase;


2,3-dihydroxyisovalerate to α-ketoisovalerate, which may be catalyzed, for example, by acetohydroxy acid dehydratase;


α-ketoisovalerate to isobutyraldehyde, which may be catalyzed, for example, by a branched-chain keto acid decarboxylase; and


isobutyraldehyde to isobutanol, which may be catalyzed, for example, by a branched-chain alcohol dehydrogenase. In some embodiments, the isobutanol biosynthetic pathway comprises at least one gene, at least two genes, at least three genes, or at least four genes that is/are heterologous to the yeast cell. In embodiments, each substrate to product conversion of an isobutanol biosynthetic pathway in a recombinant host cell is catalyzed by a heterologous polypeptide. In embodiments, the polypeptide catalyzing the substrate to product conversions of acetolactate to 2,3-dihydroxyisovalerate and/or the polypeptide catalyzing the substrate to product conversion of isobutyraldehyde to isobutanol are capable of utilizing NADH as a cofactor.


An example of a biosynthetic pathway for production of valine that can be engineered in the recombinant host cells described herein includes the steps of acetolactate conversion to 2,3-dihydroxy-isovalerate by acetohydroxyacid reductoisomerase (ILV5), conversion of 2,3-dihydroxy-isovalerate to 2-keto-isovalerate by dihydroxy-acid dehydratase (ILV3), and conversion of 2-keto-isovalerate to valine by branched-chain, amino acid transaminase (BAT2) and branched-chain amino acid aminotransferase (BAT1). Biosynthesis of leucine includes the same steps to 2-keto-isovalerate, followed by conversion of 2-keto-isovalerate to alpha-isopropylmalate by alpha-isopropylmalate synthase (LEU9. LEU4), conversion of alpha-isopropylmalate to beta-isopropylmalate by isopropylmalate isomerase (LEU1), conversion of beta-isopropylmalate to alpha-ketoisocaproate by beta-IPM dehydrogenase (LEU2), and finally conversion of alpha-ketoisocaproate to leucine by branched-chain amino acid transaminase (BAT2) and branched-chain amino acid aminotransferase (BAT1). It is desired for production of valine or leucine to overexpress at least one of the enzymes in these described pathways.


An example of a biosynthetic pathway for production of isoamyl alcohol that can be engineered in the recombinant host cells described herein includes the steps of leucine conversion to alpha-ketoisocaproate by branched-chain amino acid transaminase (BAT2) and branched-chain amino acid aminotransferase (BAT1), conversion of alpha-ketoisocaproate to 3-methylbutanal by ketoisocaproate decarboxylase (THI3) or decarboxylase ARO10, and finally conversion of 3-methylbutanal to isoamyl alcohol by an alcohol dehydrogenase such as ADH1 or SFA 1. Production of isoamyl alcohol benefits from increased production of leucine or the alpha-ketoisocaproate intermediate by overexpression of one or more enzymes in biosynthetic pathways for these chemicals. In addition, one or both enzymes for the final two steps can be overexpressed.


An example of a biosynthetic pathway for production of lactic acid that can be engineered in the recombinant host cells described herein includes pyruvate conversion to lactic acid by lactate dehydrogenase. Engineering yeast for lactic acid production using lactate dehydrogenase, known as EC 1.1.1.27, is well known in the art such as in Ishida et al. (Appl. Environ. Microbiol. 71:1964-70 (2005)).


An example of a biosynthetic pathway for production of alanine that can be engineered in the recombinant host cells described herein includes pyruvate conversion to alanine by aminotransferase.


An example of a biosynthetic pathway for production of malate that can be engineered in the recombinant host cells described herein includes pyruvate conversion to oxaloacetate by pyruvate carboxylase, and conversion of oxaloacetate to malate by malate dehydrogenase as described in Zelle et al. (Applied and Environmental Microbiology 74:2766-77 (2008)). In addition, a malate transporter can be expressed.


An example of a biosynthetic pathway for production of fumarate that can be engineered in the recombinant host cells described herein includes pyruvate conversion to oxaloacetate by pyruvate carboxylase, and conversion of oxaloacetate to malate by malate dehydrogenase as described in Zelle et al. (Applied and Environmental Microbiology 74:2766-77 (2008)). In addition, a fumarase and a fumarate transporter can be expressed. Favorable production conditions and engineering of fungi for fumarate production is well known in the art, described e.g. by Goldberg et al. (Journal of Chemical Technology and Biotechnology 81:1601 1611 (2006)).


An example of a biosynthetic pathway for production of succinate that can be engineered in the recombinant host cells described herein includes pyruvate conversion to oxaloacetate by pyruvate carboxylase, and conversion of oxaloacetate to malate by malate dehydrogenase as described in Zelle et al. (Applied and Environmental Microbiology 74:2766-77 (2008)). In addition, a fumarase, a succinate dehydrogenase and a succinate transporter can be expressed.


The skilled person will appreciate that polypeptides having activities of the above-mentioned biosynthetic pathways can be isolated from a variety of sources can be used in the recombinant host cells described herein.


It will be appreciated that host cells comprising a butanol biosynthetic pathway such as an isobutanol biosynthetic pathway as provided herein may further comprise one or more additional modifications. U.S. Appl. Pub. No. 20090305363 (incorporated by reference) discloses increased conversion of pyruvate to acetolactate by engineering yeast for expression of a cytosol-localized acetolactate synthase and substantial elimination of pyruvate decarboxylase activity. Modifications to reduce glycerol-3-phosphate dehydrogenase activity and/or disruption in at least one gene encoding a polypeptide having pyruvate decarboxylase activity or a disruption in at least one gene encoding a regulatory element controlling pyruvate decarboxylase gene expression as described in U.S. Patent Appl. Pub. No. 20090305363 (incorporated herein by reference), modifications to a host cell that provide for increased carbon flux through an Entner-Doudoroff Pathway or reducing equivalents balance as described in U.S. Patent Appl. Pub. No. 20100120105 (incorporated herein by reference). Other modifications include integration of at least one polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway. Other modifications include at least one deletion, mutation, and/or substitution in an endogenous polynucleotide encoding a polypeptide having acetolactate reductase activity. In embodiments, the polypeptide having acetolactate reductase activity is YMR226C (SEQ ID NO: 1912) of Saccharomyces cerevisae or a homolog thereof. Additional modifications include a deletion, mutation, and/or substitution in an endogenous polynucleotide encoding a polypeptide having aldehyde dehydrogenase and/or aldehyde oxidase activity. In embodiments, the polypeptide having aldehyde dehydrogenase activity is ALD6 (SEQ ID NO 1909) from Saccharomyces cerevisiae or a homolog thereof. A genetic modification which has the effect of reducing glucose repression wherein the yeast production host cell is pdc—is described in U.S. Appl. Publication No. 20110124060, incorporated herein by reference.


Recombinant host cells may further comprise (a) at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity; and (b)(i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe-S cluster biosynthesis; and/or (ii) at least one heterologous polynucleotide encoding a polypeptide affecting Fe-S cluster biosynthesis. In embodiments, the polypeptide affecting Fe-S cluster biosynthesis is encoded by AFT1 (nucleic acid SEQ ID NO: 1913, amino acid SEQ ID NO: 1914), AFT2 (SEQ ID NOs: 1915 and 1916), FRA2 (SEQ ID NOs: 1917 and 1918), GRx3(SEQ ID NOs: 1919 and 1920), or CCC1 (SEQ ID NOs: 1921 and 1922). In embodiments, the polypeptide affecting Fe-S cluster biosynthesis is constitutive mutant AFT1 L99A, AFT1 L102A, AFT1 C291F, or AFT1 C293F.


Fermentation Media

The recombinant host cells disclosed herein can be grown in fermentation media for production of a product utilizing pyruvate. For maximal production of some products, such as 2,3-butanediol, isobutanol, 2-butanone, or 2-butanol, the recombinant host cells disclosed herein used as production hosts preferably have enhanced tolerance to the produced chemical, and have a high rate of carbohydrate utilization. These characteristics can be conferred by mutagenesis and selection, genetic engineering, or can be natural.


Fermentation media for production of the products disclosed herein may contain glucose. Additional carbon substrates for product production pathways can include but are not limited to those described above. It is contemplated that the source of carbon utilized can encompass a wide variety of carbon containing substrates and will only be limited by the choice of organism.


In addition to an appropriate carbon source, fermentation media can contain suitable minerals, salts, cofactors, buffers and other components, known to those skilled in the art, suitable for the growth of the cultures and promotion of the enzymatic pathway necessary for production of the desired product.


Culture Conditions

10192 Typically cells are grown at a temperature in the range of about 20° C. to about 37° C. in, an appropriate medium. Suitable growth media for the recombinant host cells described herein are common commercially prepared media such as broth that includes yeast nitrogen base, ammonium sulfate, and dextrose as the carbon/energy source) or YPD Medium, a blend of peptone, yeast extract, and dextrose in optimal proportions for growing most Saccharomyces cerevisiae strains. Other defined or synthetic growth media may also be used and the appropriate medium for growth of the particular microorganism will be known by one skilled in the art of microbiology or fermentation science.


Suitable pH ranges for the fermentation are between pH 3.0 to pH 7.5, where pH 4.5 to pH 6.5 is preferred as the initial condition.


Fermentations can be performed under aerobic or anaerobic conditions, where anaerobic or microaerobic conditions are preferred.


The amount of product in the fermentation medium can be determined using a number of methods known in the art, for example, high performance liquid chromatography (HPLC) or gas chromatography (GC).


Industrial Batch and Continuous Fermentations

A batch method of fermentation can be used with the recombinant host cells described herein. A classical batch fermentation is a closed system where the composition of the medium is set at the beginning of the fermentation and not subject to artificial alterations during the fermentation. Thus, at the beginning of the fermentation the medium is inoculated with the desired organism or organisms, and fermentation is permitted to occur without adding anything to the system. Typically, however, a “batch” fermentation is batch with respect to the addition of carbon source and attempts are often made at controlling factors such as pH and oxygen concentration. In batch systems the metabolite and biomass compositions of the system, change constantly up to the time the fermentation is stopped. Within batch cultures cells progress through a static lag phase to a high growth log phase and finally to a stationary phase where growth rate is diminished or halted. If untreated, cells in the stationary phase will eventually die. Cells in log phase generally are responsible for the bulk of production of end product or intermediate.


A Fed-Batch system can also be used with the recombinant host cells described herein. A Fed-Batch system is similar to a typical batch system with the exception that the carbon source substrate is added in increments as the fermentation progresses. Fed-Batch systems are useful when catabolite repression (e.g. glucose repression) is apt to inhibit the metabolism of the cells and where it is desirable to have limited amounts of substrate in the media. Measurement of the actual substrate concentration in Fed-Batch systems is difficult and is therefore estimated on the basis of the changes of measurable factors such as pH, dissolved oxygen and the partial pressure of waste gases such as CO2. Batch and Fed-Batch fermentations are common and well known in the art and examples may be found in Thomas D. Brock in Biotechnology: A Textbook of Industrial Microbiology, Second Edition (1989) Sinauer Associates, Inc., Sunderland, Mass., or Deshpande, Mukund V., Appl. Biochem. Biotechnol., 36:227, (1992), herein incorporated by reference.


Although a batch mode can be performed, it is also contemplated that continuous fermentation methods could also be performed with the recombinant host cells described herein. Continuous fermentation is an open system where a defined fermentation medium is added continuously to a bioreactor and an equal amount of conditioned media is removed simultaneously for processing. Continuous fermentation generally maintains the cultures at a constant high density where cells are primarily in log phase growth.


Continuous fermentation allows for the modulation of one factor or any number of factors that affect cell growth or end product concentration. For example, one method will maintain a limiting nutrient such as the carbon source or nitrogen level at a fixed rate and allow all other parameters to vary. In other systems a number of factors affecting growth can be altered continuously while the cell concentration, measured by media turbidity, is kept constant. Continuous systems strive to maintain steady state growth, conditions and thus the cell loss due to the medium being drawn off must be balanced against the cell growth rate in the fermentation. Methods of modulating nutrients and growth factors for continuous fermentation processes as well as techniques for maximizing the rate of product formation are well known in the art of industrial microbiology and a variety of methods are detailed by Brock, supra.


It is contemplated that the present invention can be practiced using either batch, fed-batch or continuous processes and that any known mode of fermentation would be suitable. Additionally, it is contemplated that cells can be immobilized on a substrate as whole cell catalysts and subjected to fermentation conditions for production.


Product Isolation from Fermentation Medium


Products can be isolated from the fermentation medium by methods known to one skilled in the art. For example, bioproduced isobutanol may be isolated from the fermentation medium using methods known in the art for ABE fermentations (see, e.g., Dune, Appl. Microbiol. Biotechnol. 49:639-648 (1998), Groot et al., Process. Biochem. 27:61-75 (1992), and references therein). For example, solids may be removed from the fermentation medium by centrifugation, filtration, decantation, or the like. Then, the isobutanol may be isolated from the fermentation medium using methods such as distillation, azeotropic distillation, liquid-liquid extraction, adsorption, gas stripping, membrane evaporation, pervaporation or vacuum flash fermentation (see e.g., U.S. Pub. No. 20090171129 A1, and International Pub. No. WO2010/151832 A1, both incorporated herein by reference in their entirety).


Because butanol forms a low boiling point, azeotropic mixture with, water, distillation can be used to separate the mixture up to its azeotropic composition. Distillation may be used in combination with another separation method to obtain separation around the azeotrope. Methods that may be used in combination with distillation to isolate and purify butanol include, but are not limited to, decantation, liquid-liquid extraction, adsorption, and membrane-based techniques. Additionally, butanol may be isolated using azeotropic distillation using an entrainer (see, e.g., Doherty and Malone, Conceptual Design of Distillation Systems, McGraw Hill, N.Y., 2001).


The butanol-water mixture forms a heterogeneous azeotrope so that distillation may be used in combination with decantation to isolate and purify the isobutanol. In this method, the isobutanol containing fermentation broth is distilled to near the azeotropic composition. Then, the azeotropic mixture is condensed, and the isobutanol is separated from the fermentation medium by decantation. The decanted aqueous phase may be returned to the first distillation column as reflux. The isobutanol-rich decanted organic phase may be further purified by distillation in a second distillation column.


The butanol can also be isolated from the fermentation medium using liquid-liquid extraction in combination with distillation. In this method, the isobutanol is extracted from the fermentation broth using liquid-liquid extraction with a suitable solvent. The isobutanol-containing organic phase is then distilled to separate the butanol from the solvent.


Distillation in combination with adsorption can also be used to isolate isobutanol from the fermentation medium. In this method, the fermentation broth containing the isobutanol is distilled to near the azeotropic composition and then the remaining water is removed by use of an adsorbent. such as molecular sieves (Aden et al., Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover, Report NREL/TP-510-32438, National Renewable Energy Laboratory, June 2002).


Additionally, distillation in combination with pervaporation may be used to isolate and purify the isobutanol from the fermentation medium. In this method, the fermentation broth containing the isobutanol is distilled to near the azeotropic composition, and then the remaining water is removed by pervaporation through a hydrophilic membrane (Guo et al., J. Membr. Sci. 245, 199-210 (2004)).


In situ product removal (ISPR) (also referred to as extractive fermentation) can be used to remove butanol (or other fermentative alcohol) from the fermentation vessel as it is produced, thereby allowing the microorganism to produce butanol at high yields. One method for ISPR for removing fermentative alcohol that has been described in the art is liquid-liquid extraction. In, general, with regard to butanol fermentation, for example, the fermentation medium, which includes the microorganism, is contacted with an organic extractant at a time before the butanol concentration reaches a toxic level. The organic extractant and, the fermentation medium form a biphasic mixture. The butanol partitions into the organic extractant phase, decreasing the concentration in the aqueous phase containing the microorganism, thereby limiting the exposure of the microorganism to the inhibitory butanol.


Liquid-liquid extraction can be performed, for example, according to the processes described in U.S. Patent Appl. Pub. No. 20090305370, the disclosure of which is hereby incorporated in its entirety. U.S. Patent Appl. Pub. No. 20090305370 describes methods for producing and recovering butanol from a fermentation broth using liquid-liquid extraction, the methods comprising the step of contacting the fermentation broth with a water immiscible extractant to form a two-phase mixture comprising an aqueous phase and an organic phase. Typically, the extractant can be an organic extractant selected from the group consisting of saturated, mono-unsaturated, poly-unsaturated (and mixtures thereof) C12 to C22 fatty alcohols, C12 to C22 fatty acids, esters of C12 to C22 fatty acids, C12 to C22 fatty aldehydes, and mixtures thereof. The extractant(s) for ISPR can be non-alcohol extractants. The ISPR extractant can be an exogenous organic extractant such as oleyl alcohol, behenyl alcohol, cetyl alcohol, lauryl alcohol, myristyl alcohol, stearyl alcohol, 1-undecanol, oleic acid, lauric acid, myristic acid, stearic acid, methyl myristate, methyl oleate, undecanal, lauric aldehyde, 20-methylundecanal, and mixtures thereof.


In some embodiments, the alcohol can be esterfied by contacting the alcohol in a fermentation medium with an organic acid (e.g., fatty acids) and a catalyst (e.g. enzyme such as a lipase) capable of esterifying the alcohol with the organic acid. In such embodiments, the organic acid can serve as an ISPR extractant into which the alcohol esters partition. The organic acid can be supplied to the fermentation vessel and/or derived from the biomass supplying fermentable carbon fed to the fermentation vessel. Lipids present in the feedstock can be catalytically hydrolyzed to organic acid, and the same catalyst (e.g., enzymes) can esterify the organic acid with the alcohol. The catalyst can be supplied to the feedstock prior to fermentation, or can be supplied to the fermentation vessel before or contemporaneously with the supplying of the feedstock. When the catalyst is supplied to the fermentation vessel, alcohol esters can be obtained by hydrolysis of the lipids into organic acid and substantially simultaneous esterification of the organic acid with butanol present in the fermentation vessel. Organic acid and/or native oil not derived from the feedstock can also be fed to the fermentation vessel, with the native oil being hydrolyzed into organic acid. Any organic acid not esterified with the alcohol can serve as part of the ISPR extractant. The extractant containing alcohol esters can be separated from the fermentation medium, and the alcohol can be recovered from the extractant. The extractant can be recycled to the fermentation vessel. Thus, in the case of butanol production, for example, the conversion of the butanol to an ester reduces the free butanol concentration in the fermentation medium, shielding the microorganism from the toxic effect of increasing butanol concentration. In addition, unfractionated grain can be used as feedstock without separation of lipids therein, since the lipids can be catalytically hydrolyzed to organic acid, thereby decreasing the rate of build-up of lipids in the ISPR extractant.


In situ product removal can be carried out in a batch mode or a continuous mode. In a continuous mode of in situ product removal, product is continually removed from the reactor. In a batchwise mode of in situ product removal, a volume of organic extractant is added to the fermentation vessel and the extractant is not removed during the process. For in situ product removal, the organic extractant can contact the fermentation medium at the start of the fermentation forming a biphasic fermentation medium. Alternatively, the organic extractant can contact the fermentation medium after the microorganism has achieved a desired amount of growth, which can be determined by measuring the optical density of the culture. Further, the organic extractant can contact the fermentation medium at a time at which the product alcohol level in the fermentation medium reaches a preselected level. In the case of butanol production according to some embodiments of the present invention, the organic acid extractant can contact the fermentation medium at a time before the butanol concentration reaches a toxic level, so as to esterify the butanol with the organic acid to produce butanol esters and consequently reduce the concentration of butanol in the fermentation vessel. The ester-containing organic phase can then be removed from the fermentation vessel (and separated from the fermentation broth which constitutes the aqueous phase) after a desired effective titer of the butanol esters is achieved. In some embodiments, the ester-containing organic phase is separated from the aqueous phase after fermentation of the available fermentable sugar in the fermentation vessel is substantially complete.


EXAMPLES

The meaning of abbreviations used is as follows: “min” means minute(s), “h” means hour(s), “sec” means second(s), “μl” means microliter(s), “ml” means milliliter(s), “L” means liter(s), “nm” means nanometer(s), “mm” means millimeter(s), “cm” means centimeter(s), “μm” means micrometer(s), “mM” means millimolar, “M” means molar, “mmol” means millimole(s), “μmmole” means micromole(s), “g” means gram(s), “μg” means microgram(s), “mg” means milligram(s), “rpm” means revolutions per minute, “w/v” means weight/volume, “v/v” means volume/volume, “OD” means optical density, “bp” means base pair(s), and “PCR” means polymerase chain reaction.


General Methods:

Standard recombinant DNA and molecular cloning techniques used in the Examples are well known in the art and are described by Sambrook, J., Fritsch, E. F. and Mamatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989, by T. J. Sillavy, M. L. Bennan, and L. W. Enquist, Experiments' with Gene Fusions, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1984, and by Ausubel, F. M. et al., Current Protocols in Molecular Biology, Greene Publishing Assoc. and Wiley-Interscience, N.Y., 1987. Phusion® HF Master Mix (NEB Cat. No. F-531) and HotStarTaq® Master Mix (Qiagen Cat. No. 203443) were used for PCR in gene cloning and clone screening, respectively.


Materials and methods suitable for the maintenance and growth of bacterial cultures are also well known in the art. Techniques suitable for use in the following Examples may be found in Manual of Methods for General Bacteriology, Phillipp Gerhardt, R. G. E. Murray, Ralph N. Costilow, Eugene W. Nester, Willis A. Wood, Noel R. Krieg and G. Briggs Phillips, eds., American Society for Microbiology, Washington, D.C., 1994, or by Thomas D. Brock in Biotechnology: A Textbook of Industrial Microbiology, Second Edition, Sinauer Associates, Inc., Sunderland, Mass., 1989. All reagents, restriction enzymes and materials used for the growth and maintenance of bacterial cells were obtained from Aldrich Chemicals (Milwaukee, Wis.), BD Diagnostic Systems (Sparks, Md.)), Life Technologies (Rockville, Md.), or Sigma Chemical Company (St. Louis, Mo.), unless otherwise specified.


HPLC

Analysis for fermentation by-product composition is well known to those skilled in the art. For example, one high performance liquid chromatography (HPLC) method utilizes a Shodex SH-1011 column with a Shodex SH-G guard column (both available from Waters Corporation, Milford, Mass.), with refractive index (R1) detection. Chromatographic separation is achieved using 0.01 M H2SO4 as the mobile phase with a flow rate of 0.5 mL/min and a column temperature of 50° C. Isobutanol retention time is 47.6 minutes. For butanediol, meso-butanediol eluted at 26.0 min and 2R,3R-butanediol eluted at 27.7 min.


Example 1

Construction of phosphoketolase/phosphotransacetylase expression cassette. The xpk1 and eutD genes (GenBank GI numbers 28379168 (SEQ ID NO: 172) and 28377658 (SEQ ID NO: 1111), respectively) were obtained from Lactobacillus plantarum (ATCC No. BAA-793) via polymerase chain reaction (PCR) using primers N1039 and N1040 (for xpk1) and N1041 and N1042 (for eutD). The primer sequences of N1039, N1040, N1041 and N1042 correspond to SEQ ID Nos. 639-642, respectively.


The xpk1 and eutD genes were fused to a DNA fragment containing opposing yeast terminator sequences (CYC and ADH terminators, obtained from PacI digestion of pRS423::CUP1-alsS+FBA-budA, described in U.S. Patent Application Publication No. 20090155870, herein incorporated by reference) by overlap PCR method (Yu et al., Fungal Genet. Biol. 41: 973-981; 2003). The resulting PCR product was cloned into an E. coli-yeast shuttle vector using gap repair methodology (Ma et al., Genetics 58:201-216; 1981). The shuttle vector was based on pRS426 (ATCC No. 77107) and contained both GPD (also known as TDH3) and ADH1 promoters. The resulting vector contained xpk1 under control of the GPD promoter and eutD under control of the ADH1 promoter in opposing orientation. The sequence of the resulting vector (pRS426::GPD-xpk1+ADH1-eutD) is provided as SEQ ID No: 643 (see FIG. 5 for a map of this vector).


Example 2
Construction of Phosphoketolase/Phosphotransacetylase Integration Vector

An expression cassette of the pRS426::GPD-xpk1+ADH1-eutD vector (GPD-xpk1+ADH1-eutD) was prepared by digestion with EcoRI and SacI restriction enzymes. The resulting cassette was ligated into the yeast integration vector pUC19-URA3-MCS which was also prepared by digestion with EcoRI and SacI restriction enzymes.


Vector pUC19-URA3MCS is pUC19-based and contains the sequence of the URA3 gene from Saccaromyces cerevisiae situated within a multiple cloning site (MCS). pUC19 (American Type Culture Collection, Manassas, Va.; ATCC#37254) contains the pMB 1 replicon and a gene coding for beta-lactamase for replication and selection in Escherichia coli. In addition to the coding sequence for LRA3, the sequences from upstream and downstream of this gone are included for expression of the URA3 gene in yeast. The vector can be used for cloning purposes and can be used as a yeast integration vector.


The DNA encompassing the URA3 coding region along with 250 bp upstream and 150 bp downstream of the URA3 coding region from Saccaromyces cerevisiae CEN.PK 113-7D (CBS 8340; Centraalbureau voor Schimmelcultures (CBS) Fungal Biodiversity Centre, Netherlands) genomic DNA was amplified with primers oBP438 (SEQ ID NO: 644), containing BamHI, AscI, Pinel, and FseI restriction sites, and oBP439 (SEQ ID NO: 645), containing XbaI, P acI, and NotI restriction sites. Genomic DNA was prepared using a Gentra Puregene Yeast/Bact kit (Qiagen). The PCR product and, pUC19 were ligated with T4 DNA ligase after digestion with BamHI and XbaI to create vector pUC19-URA3MCS. The vector was confirmed by PCR and sequencing with primers oBP264 (SEQ ID NO:646) and oBP265 (SEQ ID NO:647).


The ligation reaction was transformed into E. coli Stb13 cells, according to the manufacturer's instructions (Invitrogen, Carlsbad, Calif., Cat. No. C7373). Transformants were screened by polymerase chain reaction (PCR) to detect the eutD gene using the primers N1041 and N1042 (SEQ ID NOs: 641 and 642, respectively). Positive clones for eutD gene expression detected by PCR were further confirmed for eutD gene incorporation by digestion of the vector with SacII restriction enzyme.


Two confirmed clones were selected and an integration targeting sequence was added to the clones as follows. PCR was used to amplify regions of the genome of S. cerevisiae strain BY4700 (ATCC No. 200866) both 5′ and 3′ of the PDC1 gene using the following primers: N1049 and N1050 (5′) and N1047 and N1048 (3′) (SEQ ID NOs: 648-651, respectively). Primer N1049 enables the 3′ end of the 161-bp PDC1 3′ sequence to be fused to the 5′ end of the 237 bp PDC1 5′ sequence via PCR. This pdc 1 3′-5′-fusion fragment (368 by in length) was cloned into the pCR11—Blunt TOPO vector according to the manufacturer's instructions (Invitrogen, Carlsbad, Calif., Cat. No. K2800).


Transformants were screened by PCR to detect the pdc1 3′-5′-fusion fragment using primers N1047 and N1050. The pdc1 3′-5′-fusion fragment was isolated from positive clones and released from the vector by digestion with EcoRI enzyme, and ligated into a pUC19-URA3::GPD-xpk1+ADH-eutD vector that had been linearized by digestion with EcoRI restriction enzyme to generate the “phosphoketolase pathway” vector. Additionally, the pdc1 3′-5′-fusion fragment was ligated with pUC19-URA3-MCS digested with EcoRI restriction enzyme to generate the control vector. Both ligation reactions were transformed into E. coli Stb13 cells according to the manufacturer's instructions (Invitrogen, Carlsbad, Calif., Cat. No. C7373). The resulting transformants were screened by PCR to detect the pdc1 3′-5′-fusion fragment using primers N1047 and N1050. Positive clones containing the pdc1 3′-5′-fusion fragment were identified and the vectors were digested with either NcoI restriction enzyme (control vector) or BsgI restriction enzyme (phosphoketolase pathway vector) to confirm cloning orientation. One control clone (=pUC19-URA3::pdc1) and one phosphoketolase pathway clone (=pUC19-URA3::pde1::GPD-xpk1+ADH1-eutD; SEQ ID NO: 1898) were selected for integration.


Example 3
Construction of Pyruvate Decarboxylase Knockout (PDC-KO) Yeast Strain Containing Phosphoketolase and Phosphotransacetylase Genes

The control and phosphoketolase pathway vectors described in Example 2 were linearized with AflII restriction enzyme and transformed into strain BP913 (CEN.PK113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvD(Sm) Δpc-1c5::sadB) to form control and phosphoketolase pathway strains. Strain BP913 is further described in Example 10.


Transformed cells were plated on synthetic complete medium without uracil containing ethanol as the sole carbon source (1% vol/vol) and screened by PCR using primers N238 and oBP264 (SEQ ID Nos. 652 and 646, respectively to confirm integration at the pdc1 locus. Integration at the Δpdc1::ilvD(Sm) locus resulted in the loss of ilvD(Sm).


Example 4
Introduction of Phosphoketolase and Phosphotransacetylase Allows Growth of PDC-KO Cells without Exogenous Two-Carbon Substrate Supplementation

Pyruvate decarboxylase knockout (PDC-KO) yeast strains are unable to grow in media containing 2% glucose as the sole carbon source, but can grow in 2% glucose supplemented with ethanol as shown with a strain transformed with one or more plasmids encoding members of the butanediol pathway (described in U.S. Patent Application Publication No. 20090305363, herein incorporated by reference). To test whether the introduction of the phosphoketolase and phosphotransacetylase genes could support growth of PDC-KO cells, PDC-KO yeast were transformed with the phosphoketolase and phosphotransacetylase gene (as described in Example 3) and with the vector pRS423CUP1-alsS+F13A-budA (described in U.S. Patent Application Publication No 20090155870, herein incorporated by reference) encoding members of the butanediol pathway. After cultivation in media containing 2% glucose (synthetic complete minus his and ura) supplemented with 0.05% v/v ethanol, cultures were diluted into the same media lacking ethanol (starting OD=0.1, 20 ml medium in a 125 ml vented flask). For comparison, a control PDC-KO strain without introduction of the phosphoketolase and phosphotransacetylase genes was also diluted into medium supplemented with ethanol (0.05% vol/vol). The optical density at 600 nm was measured during growth (results shown in FIG. 2 and Table 13).











TABLE 13









Condition











Strain
0 h OD
16 h OD
22 h OD
41.3 h OD














xpk A
0.1
2.07
5.63
9.64


xpk B
0.1
2.44
5.93
9.78


xpkC
0.1
2.26
5.83
9.96


control A
0.1
0.47
0.5
0.822


control B
0.1
0.45
0.51
0.849


control C
0.1
0.5
0.52
0.879


cont A w/EtOH
0.1
2.01
5.49
11.44


cont B w/EtOH
0.1
2.16
5.7
11.5


cont C w/EtOH
0.1
2.12
5.76
11.76









The growth of PDC-KO yeast transformed with phosphoketolase and phosphotransacetylase in media that was not supplemented with ethanol (xpkA-xpkC, representing n=3 results) was indistinguishable from the growth of PDC-KO yeast strains grown in media containing 2% glucose that was supplemented with ethanol (cont A-cont C w/EtOH, representing n=3 results). The average growth rate of the phosphoketolase- and phosphotransacetylase-transformed strains under these conditions was 0.19 h−1, A growth rate of 0.23 h−1 for the phosphoketolase- and phosphotransacetylase-transformed strains was observed upon culturing under the same conditions with higher aeration (data not shown). PDC-KO yeast strains grown in media containing 2% glucose that was not supplemented with ethanol showed some growth in the first 16 hours, but then grew at a rate of only 0.01 h−1 (control A-control C, representing n=3 results).


Example 5
Construction of Pyruvate Decarboxylase Knockout (PDC-KO) Yeast Strains Containing Either Phosphoketolase or Phosphotransacetylase Genes

The integration vector described above (pUC19-URA3::pdc1::GPD-xpk1+ADH1-eutD) was modified to eliminate either the xpk1 phosphoketolase gene or the eutD phosphotransacetylase gene. Specifically, to remove eutD, the integration vector was digested with the Gal and SpeI restriction enzymes to remove a 0.6 kb region from the eutD coding sequence, forming the vector pUC19-URA3::pdc1::GPD-xpk1. To remove xpk1, the integration, vector was digested with the SpeI and KpnI restriction enzymes to remove the 3.4 kb region from SpeI to KpnI, forming the vector pUC19-URA3::pdc1::ADH-eutD. The resulting vectors, were linearized with digestion with the AflII restriction enzyme and transformed into B1-913/pRS423::CUP1-alsS+FBA-budA cells (described in Example 3). Transformed cells were screened by PCR to confirm integration at the pdc1 locus and cultured, as described above.


Example 6
Introduction of Phosphoketolase Allows Growth of PDC-KO Cells without Exogenous Two Carbon Substrate Supplementation

To test whether the introduction of either the phosphoketolase or phosphotransacetylase genes could support the growth of PDC-KO cells, PDC-KO yeast were transformed with either the phosphoketolase or phosphotransacetylase genes (as described in Example 5) and with the vector pRS423::CUP1-alsS+FBA-budA encoding members of the butanediol pathway (as described in Example 4). After cultivation in media containing 2% glucose (synthetic complete minus his and ura) supplemented with 0.05% v/v ethanol, cultures were diluted into the same media lacking ethanol (starting OD=0.1, 20 ml medium in a 125 ml vented flask). For comparison, a PDC-KO strain without introduction of the phosphoketolase or phosphotransacetylase genes were grown under the same conditions. The optical density at 600 nm was measured during growth (results shown in FIG. 3 and Table 15).













TABLE 15







Strain
0 h OD
24 h OD




















xpk1 + eutD
0.1
7.48



none (control)
0.1
0.575



eutD only
0.1
0.338



eutD only
0.1
0.28



xpk1 only
0.1
6.74



xpk1 only
0.1
7.26










The growth of PDC-KO yeast transformed with phosphoketolase in media that was not supplemented with exogenous carbon substrate (xpk1, FIG. 3) was indistinguishable from the growth of PDC-KO yeast transformed with phosphoketolase and phosphotransacetylase grown in media containing 2% glucose that was supplemented with ethanol (xpk1+eutD, FIG. 3). The growth of PDC-KO yeast transformed with phosphotransacetylase (eutD, FIG. 3) was not significantly improved compared to PDC-KO yeast strains in media that was not supplemented with exogenous two-carbon substrate (none, FIG. 3).


Example 7
Introduction of Phosphoketolase to PDC-KO Cells Increases Glucose Consumption and Butanediol Yield

To test the effects of introduction of p iosphoketolase into PDC-KO cells on glucose consumption and butanediol yield, PDC-KO yeast ransformed with either (1) phosphoketolase and phosphotransacetylase (as described in Example 4) and the vector pRS423::CUP1-alsS+FBA-budA encoding members of the butanediol (BDO pRS423::CUP1-alsS+FBA-budA encoding members of the butanediol pathway (“Control” in Table 6 below).


After cultivation in medium containing 2% glucose (synthetic complete minus histidine and uracil) supplemented with 0.05% ethanol, Xpk and Control cultures were diluted into medium without ethanol (starting OD=0.1, 20 ml, medium in a 125 ml vented flask). Glucose consumption and butanediol yield of Xpk and Control cultures were measured by HPLC analysis of culture media for amount of glucose and butanediol as shown in the Table below.









TABLE 16







Introduction of Phosphoketolase Increases Glucose


Consumption and Butanediol Yield of PDC-KO Cells.












Glucose consumed
Butanediol



Strains
(mM)
Molar Yield







Xpk (n = 3)
73.9 ± 2.4
0.475 ± 0.001



Control (n = 3)
48.3 ± 0.6
0.359 ± 0.003










The glucose consumption of Xpk cells (n=3) was nearly twice the amount of glucose consumption of control strains (n=3). In addition, the butanediol molar yield of Xpk cells was increased compared to the butanediol molar yield of Control cells.


Example 8
Construction of an Additional Phosphoketolase Pathway Integration Vector

A phosphoketolase/phosphotransacetylase integration vector similar to the one described in Example 2 was constructed. In this case the xpk1 and eutD gene constricts were cloned so that they would be integrated immediately downstream of the Δpdc1::ilvD(Sm) locus of BP913. To do this, the intergenic region between ilvD(Sm) and TRX1 was amplified from BP913 genomic DNA using primers N1110 and N1111 (SEQ ID Nos. 653 and 654). This was cloned into pUC19-URA3-MCS at the Pmel site, as follows. The ilvD-TRX1 PCR product was phosphorylated with polynucleotide kinase (NEB Cat. No. MO201), the vector was prepared by digesting with PmeI and treating with calf intestinal phosphatase, the two fragments were ligated overnight and cloned into E. coli Stb13 cells. Clones were screened by PCR (using N1110 and N1111 primers) and then digested with BsgI to determine the orientation of the ilvD-TRX1 insertion. One clone from each orientation (pUC19-URA3::ilvD-TRX1 A and B was carried over to the next step: addition of the xpk1/eutD expression cassette. The xpk1/eutD expression cassette from pRS426::GPD-xpk1+ADH1-eutD was obtained by digestion with BglII and EcoRV. The 5′ overhanging DNA was filled in using Klenow Fragment. pUC19-URA3::ilvD-TRX1 was linearized with AflII and the 5′ overhanging DNA was filled in using Klenow fragment. This vector was then ligated with the prepared xpk1/eutD cassette. Ligation reactions were transformed into E. coli Stb13 cells. Clones were screened using primers for evil) (N1041 and N1042) and then digested with Ban to determine orientation of the xpk1/eutD cassette relative to the ilvD-TRXJ DNA sequence.


The URA3 marker gene was then replaced with a geneticin resistance marker as follows. A chimeric geneticin resistance gene was constructed that contained the Kluyveromyces lactis TEF1 promoter and terminator (TEF1p-kan-TEF1t gene, provided as SEQ ID No. 655). This gene was maintained in a pUC19 vector (cloned at the SmaI site). The kan gene was isolated from pUC19 by first digesting with KpnI, removal of 3′ overhanging DNA using Klenow Fragment (NEB, Cat. No. M212), digesting, with HincII and then gel purifying the 1.8 kb gene fragment (Zymoclean™ Gel DNA Recovery Kit, Cat. No. D4001, Zymo Research, Orange, Calif.). The URA3 marker was removed from pUC19-URA3::ilvD::GPD-xpk1+ADH1-eutD::TRX1 (paragraph above) using NsiI and NaeI (the 3′ overhanging DNA from NsiI digestion was removed with Klenow fragment). The vector and kan gene were ligated overnight and transformed into E. coli Stb13 cells. Clones were screened by PCR using primers BK468 and either N1090 or N1113 (SEQ ID Nos. 656, 657, and 658, respectively)—positive PCR results indicate presence and orientation of kan gene. Clones in both orientations were digested with PmeI and transformed into BP913 with selection on yeast extract-peptone medium supplied with 1% (v/v) ethanol as carbon source and 200 pg/ml geneticin (G418). A single transformant was obtained, as confirmed by PCR (primers N886 and oBP264 for the 5′ end N1090 and oBP512 for the 3′ end, SEQ ID Nos.659, 646, 657, and 660, respectively). FIG. 6 depicts the locus after integration of the plasmid.


Example 9
Construction of an Isobutanol-Producing Strain Carrying the Phosphoketolase Pathway

The strain described in Example 8 was transformed with 2 plasmids containing genes for an isobutanol pathway pYZ090 and pYZ067 (SEQ ID NOs: 1892 and 1891).


pYZ090 was constructed to contain a chimeric gene having the coding region of the alsS gene from Bacillus subtilis (nt position 457-2172) expressed from the yeast CUP1 promoter (nt 2-449) and followed by the CYC1 terminator (nt 2181-2430) for expression of ALS, and a chimeric gene having the coding region of the ilvC gene from Lactococcus lactis (nt 3634-4656) expressed from the yeast ILV5 promoter (2433-3626) and followed by the ILV5 terminator (nt 4682-5304) for expression of KARI. pYZ067 was constructed to contain the following chimeric genes: 1) the coding region of the ilvD gene from S. mutans UA159 with a C-terminal Lumio tag (nt 2260-3972) expressed from the yeast FBA1 promoter (nt 1661-2250) followed by the FBA1 terminator (nt 40005-4317) for expression of dihydroxy acid dehydratase, 2) the coding region for horse liver ADH (nt 4680-5807) expressed from the yeast GPM1 promoter (nt 5819-6575) followed by the ADH1 terminator (nt 4356-4671) for expression of alcohol dehydrogenase, and 3) the coding region of the kivD gene from Lactococcus lactis (nt 7175-8821) expressed from the yeast TDH3 promoter (nt 8830-9493) followed by the TDH3 terminator (nt 6582-7161) for expression of ketoisovalerate decarboxylase.


Transformants were obtained on synthetic complete medium lacking uracil and histidine with 1% (v/v) ethanol as carbon source and 100 pg/ml geneticin. Control strains (BP913) were also transformed with the same plasmids and plated without geneticin. A number of transformants were then patched to the same medium containing 2% glucose as carbon source and supplemented with 0.05% (v/v) ethanol. After 36 hours, patches were used to inoculate liquid medium (same composition as the plates). After 48 hours, ODs for both phosphoketolase pathway and control strains were similar (ca. 4-5 OD) and all were subcultured into medium lacking ethanol (i.e. no exogenous two-carbon substrate source). The phosphoketolase cultures grew without ethanol supplementation, similar to ethanol supplemented control strains. Results are shown in FIG. 7A (and Table 17A). These were subcultured again to confirm growth rates, and results are shown in FIG. 7B (and Table 17B). Phosphoketolase strains appeared to have a decreased lag phase compared to controls, but the exponential growth rates were not statistically different (average rate of 0.16h−1).












TABLE 17A









Condition












Strain
0 h OD
18.3 h OD















xpk ISO 1
0.1
2.3



xpk ISO 2
0.1
2.2



xpk ISO 3
0.1
2.2



ISO (no EtOH) 1
0.1
0.48



ISO (no EtOH) 2
0.1
0.41



ISO (no EtOH) 3
0.1
0.47



ISO (+EtOH) 1
0.1
2.5



ISO (+EtOH) 2
0.1
2.6



ISO (+EtOH) 3
0.1
2.4



















TABLE 17B









Condition















0 h
6.5 h
23 h
27 h
48 h



Strain
OD
OD
OD
OD
OD


















xpk ISO 1
0.1
0.18
2.91
4
4.4



xpk ISO 2
0.1
0.14
1.54
2.88
4.6



xpk ISO 3
0.1
0.16
2.15
3.54
4.3



ISO (+EtOH) 1
0.1
0.14
2.21
3.46
4.4



ISO (+EtOH) 2
0.1
0.11
1.13
2.18
4.2



ISO (+EtOH) 3
0.1
0.1
0.84
1.6
4.4










Example 10
Construction of Saccharomyces cerevisiae Strain BP913

The purpose of this example is to describe the construction of Saccharomyces cerevisiae strain BP913. The strain was derived from CEN.PK 113-7D (CBS 8340; Centraalbureau voor Schimmelcultures (CBS) Fungal Biodiversity Centre, Netherlands) and contains deletions of the following genes: URA3, HISS, PDC1, PDC5, and PDC6.


Deletions, which completely removed the entire coding sequence, were created by homologous recombination with PCR fragments containing regions of homology upstream and downstream of the target gene and either a G418 resistance marker or URA3 gene for selection of transformants. The G418 resistance marker, flanked by loxP sites, was removed using Cre recombinase. The URA3 gene was removed by homologous recombination to create a scarless deletion.


In general, the PCR cassette for each scarless deletion was made by combining four fragments, A-B-U-C, by overlapping PCR. The PCR cassette contained a selectable/counter-selectable marker, URA3 (Fragment U), consisting of the native CEN.PK 113-7D URA3 gene, along with the promoter (250 bp upstream of the URA3 gene) and terminator (150 bp downstream of the URA3 gene). Fragments A and C, each 500 by long, corresponded to the 500 by immediately upstream of the target gene (Fragment A) and the 3 500 by of the target gene (Fragment C). Fragments A and C were used for integration of the cassette into the chromosome by homologous recombination. Fragment B (500 by long) corresponded to the 500 by immediately downstream of the target gene and was used for excision of the URA3 marker and Fragment C from the chromosome by homologous recombination, as a direct repeat of the sequence corresponding to Fragment B was created upon integration of the cassette into the chromosome. Using the PCR product ABUC cassette, the URA3 marker was first integrated into and then excised from the chromosome by homologous recombination. The initial integration deleted the gene, excluding the 3′ 500 bp. Upon excision, the 3′ 500 by region of the gene was also deleted. For integration of genes using this method, the gene to be integrated was included in the PCR cassette between fragments A and B. URA3 Deletion


To delete the endogenous URA3 coding region, a ura3::loxP-kanMX-loxP cassette was PCR-amplified from pLA54 template DNA (SEQ ID NO: 661). pLA54 contains the K. lactis TEF1 promoter ar d kanMX marker, and is flanked by loxP sites to allow recombination with Cre recombinase and removal of the marker. PCR was done using Phusion DNA polymerase and primers BK505 and BK506 (SEQ ID NOs:662 and 663). The URA3 portion of each primer was derived from the 5′ region upstream of the URA3 promoter and 3′ region downstream of the coding region such that integration of the loxP-kanMX-loxP marker resulted in replacement of the URA3 coding region. The PCR product was transformed into CEN.PK 113-7D using standard genetic techniques (Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202) and transformants were selected on YPD containing 0418 (100 pg/ml) at 30 C. Transformants were screened to verify correct integration by PCR using primers LA468 and LA492 (SEQ ID NOs: 664 and 665) and designated CEN.PK 113-7D Δura3::kanMX.


HIS3 Deletion

The four fragments for the PCR cassette for the scarless HIS3 deletion were amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs) and CEN.PK 113-7D genomic DNA as template, prepared with a Centra Puregene Yeast/Bact kit (Qiagen; Valencia, Calif.). HIS3 Fragment A was amplified with primer oBP452 (SEQ ID NO: 666) and primer oBP453 (SEQ ID NO: 667), containing a 5′ tail with homology to the 5′ end of HIS3 Fragment B. HIS3 Fragmeht B was amplified with primer oBP454 (SEQ ID NO: 668), containing a 5′ tail with homology to the 3′ end of HIS3 Fragment A, and primer oBP455 (SEQ ID NO: 669), containing a 5′ tail with homology to the 5′ end of HIS3 Fragment U. HIS3 Fragment U was amplified with primer oBP456 (SEQ ID NO: 670), containing a 5′ tail with homology to the 3′ end of HIS3 Fragment B, and primer oBP457 (SEQ ID NO: 671), containing a 5′ tail with homology to the 5′ end of HIS3 Fragment C. HIS3 Fragment C was amplified with primer oBP458 (SEQ ID NO: 672), containing a 5′ tail with homology to the 3′ end of HIS3 Fragment U, and primer oBP459 (SEQ ID NO: 673). PCR products were purified with a PCR Purification kit (Qiagen, Valencia, Calif.). HIS3 Fragment AB was created by overlapping PCR by mixing HIS3 Fragment A and HIS3 Fragment B and amplifying with primers oBP452 (SEQ ID NO: 666) and oBP455 (SEQ ID NO: 669). HIS3 Fragment UC was created by overlapping PCR by mixing HIS3 Fragment U and HIS3 Fragment C and amplifying with primers oBP456 (SEQ ID NO: 670) and oBP459 (SEQ ID NO: 673). The resulting PCR products were purified on an agarose gel followed by a Gel Extraction kit (Qiagen, Valencia, Calif.). The HIS3 ABUC cassette was created by overlapping PCR by mixing HIS3 Fragment AB and HIS3 Fragment UC and amplifying with primers oBP452 (SEQ ID NO: 666) and oBP459 (SEQ ID NO: 673). The PCR product was purified with a PCR Purification kit (Qiagen, Valencia, Calif.).


Competent cells of CEN.PK 113-71) Δura3::kanMX were made and transformed with the HIS3 ABUC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research, Orange, Calif.). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 2% glucose at 30 C. Transformants with a his knockout were screened for by PCR with primers oBP460 (SEQ ID NO: 674) and oBP461 (SEQ ID NO: 671) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). A correct transformant was selected as strain CEN.PK 113-7D Δura3::kanMX Δhis3::URA3.


KanMX Marker Removal from the Δura3 Site and URA3 Marker Removal from the Δhis3 Site


The KanMX marker was removed by transforming CEN.PK 113-7D Δura3::kanMX Δhis3::URA3 with pRS423::PGAL1-cre (SEQ ID NO: 715) using a Frozen-EZ Yeast Transformation II kit (Zymo Research, Orange, Calif.) and plating, on synthetic complete medium lacking histidine and uracil supplemented with 2% glucose at 30 C. Transformants were grown in YP supplemented with galactose at 30 C for ˜6 hours to induce the Cre recombinase and KanMX marker excision and plated onto YPD (22 u glucose) plates at 30 C for reekwery. An isolate was grown overnight in YPD and plated on synthetic complete medium containing 5-fluoro-orotic acid (0.1%) at 30 C to select for isolates that lost the URA3 marker, 5-FOA resistant isolates were grown in and plated on YPD for removal of the pRS423.:PGAL1-cre plasmid. Isolates were checked for loss of the KanMX marker, URA3 marker, and pRS423::PGAL1-erc plasmid by assaying, growth on YPD+G418 plates. synthetic complete medium lacking uracil plates. and synthetic complete medium lacking histidine plates. A correct isolate that was sensitive to G418 and auxotrophic for uracil and histidine was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 and designated as BP857. The deletions and marker removal were confirmed by PCR and sequencing with primers oB13450 (SEQ ID NO: 676) and oBP451 (SEQ ID NO: 677) for Δura3 and primers oBP460 (SEQ ID NO: 674) and oBP461 (SEQ ID NO: 675) for Δhis3 using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen, Valencia, Calif.).


PDC6 Deletion

The four fragments for the PCR cassette for the starless PDC6 deletion were amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs, Ipswich, Mass.) and CEN.PK 113-7D genomic DNA as template, prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). PDC6 Fragment A was amplified with primer oBP440 (SEQ ID NO: 670) and primer oBP441 (SEQ ID NO: 679), containing a 5′ tail with homology to the 5′ end of PDC6 Fragment B. PDC6 Fragment B was amplified with primer oBP442 (SEQ ID NO: 680), containing a 5′ tail with homology to the 3′ end of PDC6 Fragment A, and primer oBP443 (SEQ ID NO: 681), containing, a 5′ tail with homology to the 5′ end of PDC6 Fragment U. PDC6 Fragment U was amplified with primer oBP444 (SEQ ID NO: 682), containing a 5′ tail with homology to the 3′ end of PDC6 Fragment B, and primer oBP445 (SEQ ID NO: 683), containing a 5′ tail with homology to the 5′ end of PDC6 Fragment C. PDC6 Fragment C was amplified with primer oBP446 (SEQ ID NO: 684), containing a 5′ tail with homology to the 3′ end of PDC6 Fragment U, and primer oBP447 (SEQ ID NO: 685). PCR products were purified with, a PCR Purification kit (Qiagen). PDC6 Fragment AB was created by overlapping PCR by mixing PDC6 Fragment A and PDC6 Fragment B and amplifying with primers oBP440 (SEQ ID NO: 678) and oBP443 (SEQ ID NO: 681). PDC6 Fragment UC was created by overlapping PCR by mixing PDC6 Fragment U and PDC6 Fragment C and amplifying with primers oBP444 (SEQ ID NO: 682) and oBP447 (SEQ ID NO: 685). The resulting PCR products were purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The PDC6 ABUC cassette was created by overlapping PCR by mixing PDC6 Fragment AB and PDC6 Fragment UC and amplifying with primers oBP440 (SEQ ID NO: 678) and oBP447 (SEQ ID NO 685). The PCR product was purified with a PCR Purification kit (Qiagen).


Competent cells of CEN.PK 113-7D Δura3::loxP Δhis3 were made and transformed with the PDC6 ABUC PCR cassette using a Frozen-E2 Yeast Transformation II kit (Zymo Research), Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 2% glucose at 30 C. Transformants with a pdc6 knockout were screened for by PCR with primers oBP448 (SEQ ID NO 686) and oBP449 (SEQ ID NO; 687) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). A correct transformant was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6::URA3.


CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6::URA3 was grown overnight in YPD and plated on synthetic complete medium containing 5-fluoro-orotic acid (0,1%) at 30 C to select for isolates that lost the URA3 marker. The deletion and marker removal were confirmed by PCR and sequencing with primers oBP448 (SEQ ID NO: 686) and oBP449 (SEQ ID NO 687) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The absence of the PDC6 gene from the isolate was demonstrated by a negative PCR result using primers specific for the coding sequence of PDC6, oBP554 (SEQ ID NO 688) and oBP555 (SEQ ID NO 689). The correct isolate was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 and designated as BP891.


PDC1 Deletion ilvDSm Integration


The PDC1 gene was deleted and replaced with the ilvD coding region from Streptococcus mutans ATCC #700610 (SEQ ID NO: 1886). The A fragment followed by the ilvD coding region from Streptococcus mutans for the PCR cassette for the PDC1 deletion-ilvDSm integration was amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs) and NYLA83 genomic DNA as template (construction of strain NYLA83 is described in U.S. Application Pub. No. 20110124060 A1, incorporated herein by reference), prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). PDC1 Fragment A-ilvDSm was amplified with primer oBP513 (SEQ ID NO: 690) and primer oBP515 (SEQ ID NO: 691), containing a 5′ tail with homology to the 5′ end of PDC1 Fragment B. The B, U, and C fragments for the PCR cassette for the PDC1 deletion-ilvDSm integration were amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs) and CEN.PK 113-7D genomic DNA as template, prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). PDC1 Fragment B was amplified with primer oBP516 (SEQ ID NO: 692), containing a 5′ tail with homology to the 3′ end of PDC1 Fragment A-ilvDSm, and primer oBP517 (SEQ ID NO: 693), containing a 5′ tail with homology to the 5′ end of PDC1 Fragment U. PDC1 Fragment U was amplified with primer oBP518 (SEQ ID NO: 694), containing a 5′ tail with homology to the 3′ end of PDC1 Fragment B, and primer oBP519 (SEQ ID NO: 695), containing a 5′ tail with homology to the 5′ end of PDC1 Fragment C. PDC1 Fragment C was amplified with primer oBP520 (SEQ ID NO: 696), containing a 5′ tail with homology to the 3′ end of PDC1 Fragment U, and primer oBP521 (SEQ ID NO: 697). PCR products were purified with a PCR Purification kit (Qiagen). PDC1 Fragment A-ilvDSm-B was created by overlapping PCR by mixing PDC1 Fragment A-ilvDSrn and PDC1 Fragment B and amplifying with primers oBP513 (SEQ ID NO: 690) and oBP517 (SEQ ID NO: 693). PDC Fragment UC was created by overlapping PCR by mixing PDC1 Fragment U and PDC1 Fragment C and amplifying with primers oBP518 (SEQ ID NO: 694) and oBP521 (SEQ ID NO: 697). The resulting PCR products were purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The PDC 1 A-ilvDSm-BUC cassette was created by overlapping PCR by mixing PDC 1 Fragment A-ilvDSm-B and PDC1 Fragment UC and amplifying with primers oBP513 (SEQ ID NO 690) and oBP521 (SEQ ID NO: 697). The PCR product was purified with a PCR Purification kit (Qiagen).


Competent cells of CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 were made and transformed with the PDC1 A-ilvDSm-BUC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 2% glucose at 30 C. Transformants with a pdc1 knockout ilvDSm integration were screened for by PCR with primers oBP511 (SEQ ID NO: 698) and oBP512 (SEQ ID NO: 699) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The absence of the PDC1 gene from the isolate was demonstrated by a negative PCR result using primers specific for the coding sequence of PDC1, oBP550 (SEQ ID NO: 700) and oBP551 (SEQ ID NO: 701). A correct transformant was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc 1::ilvDSm-URA3.


CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm-URA3 was grown overnight in YPD and plated on synthetic complete medium containing 5-fluoro-orotic acid (0.1%) at 30 C to select for isolates that lost the URA3 marker. The deletion of PDC1, integration of ilvDSm, and marker removal were confirmed by PCR and sequencing with primers oBP511 (SEQ ID NO: 698) and oBP512 (SEQ ID NO: 699) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The correct isolate was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm and designated as BP907.


PDC5 Deletion sadB Integration


The PDC5 gene was deleted and replaced with the sadB coding region from Achromobacter xylosoxidans. A segment of the PCR cassette for the PDC5 deletion-sadB integration was first cloned into plasmid pUC19-URA3MCS (described in Example 2). The coding sequence of sadB (SEQ ID NO: 718) and PDC5 Fragment B were cloned into pUC19-URA3MCS to create the sadB-BU portion of the PDC5 A-sadB-BUC PCR cassette. The coding sequence of sadB was amplified using pLH468-sadB (SEQ ID NO: 716) as template with primer oBP530 (SEQ ID NO: 702), containing an AscI restriction site, and primer oBP531 (SEQ ID NO: 703), containing a 5′ tail with homology to the 5′ end of PDC5 Fragment B. PDC5 Fragment B was amplified from CEN.PK 113-7D genomic DNA with primer oBP532 (SEQ ID NO: 704), containing a 5′ tail with homology to the 3′ end of sadB, and primer oBP533 (SEQ ID NO: 705), containing a PmeI restriction site PCR products were purified with a PCR Purification kit (Qiagen). sadB-PDC5 Fragment B was created by overlapping PCR by mixing the sadB and PDC5 Fragment B PCR products and amplifying with primers oBP530 (SEQ ID NO: 702) and oBP533 (SEQ ID NO 705). The resulting PCR product was digested with Asci and PmeI and ligated with T4 DNA ligase into the corresponding sites of pUC19-URA3MCS after digestion with the appropriate enzymes. The resulting plasmid was used as a template for amplification of sadB-Fragment B-Fragment U using primers oBP536 (SEQ ID NO: 706) and oBP546 (SEQ ID NO: 707), containing a 5′ tail with homology to the 5′ end of PDC5 Fragment C. PDC5 Fragment C was amplified from CEN.PK 113-7D genomic DNA with primer oBP547 (SEQ ID NO: 708), containing a 5′ tail with homology to the 3′ end of PDC5 sadB-Fragment B-Fragment U, and primer oBP539 (SEQ ID NO: 709). PCR products were purified with a PCR Purification kit (Qiagen). PDC5 sadB-Fragment B-Fragment U-Fragment C was created by overlapping PCR by mixing PDC5 sadB-Fragment B-Fragment U and PDC5 Fragment C and amplifying with primers oBP536 (SEQ ID NO: 706) and oBP539 (SEQ II) NO: 709). The resulting PCR product was purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The PDC5 A-sadB-BUC cassette was created by amplifying PDC5 sadB-Fragment B-Fragment U-Fragment C with primers oBP542 (SEQ ID NO: 710) containing a 5′ tail with homology to the 50 nucleotides immediately upstream of the native PDC5 coding sequence, and oBP539 (SEQ ID NO: 709). The PCR product was purified with a PCR Purification kit (Qiagen).


Competent cells of CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm were made and transformed with the PDC5 A-sadB-13UC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 1% ethanol (no glucose) at 30 C. Transformants with a pdc5 knockout sadB integration were screened for by PCR with primers oBP540 (SEQ ID NO: 711) and oBP541 (SEQ ID NO: 712) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The absence of the PDC5 gene from the isolate was demonstrated by a negative PCR result using primers specific for the coding sequence of PDC5, oBP552 (SEQ ID NO: 713) and oBP553 (SEQ ID NO: 714). A correct transformant was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3


Δpdc6 Δpdc1::ilvDSrn Δpdc5::sadB-URA3.


CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm Δpdc5::sad13-URA3 was grown overnight in YPE (1% ethanol) and plated on synthetic complete medium supplemented with ethanol (no glucose) and containing 5-fluoro-orotic acid (0.1%) at 30 C to select for isolates that lost the URA3 marker. The deletion, of PDC5, integration of sadB, and marker removal were confirmed by PCR with primers oBP540 (SEQ ID NO 711) and oBP541 (SEQ ID NO: 712) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The correct isolate was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm Δpdc5::sadB and designated as BP913.


Example 11
Construction of Strain NYLA83

This example describes insertion-inactivation of endogenous PDC1 and PDC6 genes of S. cerevisiae. PDC1, PDC5, and PDC6 genes encode the three major isozymes of pyruvate decarboxylase. The resulting strain was used as described in Example 10.


Construction of pRS425::GPM-sadB


A DNA fragment encoding a butanol dehydrogenase (SEQ ID NO: 717) from Achromobacter xylosoxidans (disclosed in US Patent Application Publication No. US20090269823) was cloned. The coding region of this gene called sadB for secondary alcohol dehydrogenase (SEQ ID NO: 718) was amplified using standard conditions from A. xylosoxidans genomic DNA, prepared using a Gentra Puregene kit (Gentra Systems, Inc., Minneapolis, Minn.; catalog number D-5500A) following the recommended protocol for gram negative organisms using forward and reverse primers N473 and N469 (SEQ ID NOs:725 and 726), respectively. The PCR product was TOPO-Blunt cloned into pCR4 BLUNT (Invitrogen) to produce pCR4Blunt::sadB, which was transformed into E. coli Mach-1 cells. Plasmid was subsequently isolated from four clones, and the sequence verified.


The sadB coding region was PCR amplified from pCR4Blunt::sadB. PCR primers contained additional 5′ sequences that would overlap with the yeast GPM 1 promoter and the ADH1 terminator (N583 and N584, provided as SEQ ID NOs:727 and 728). The PCR product was then cloned using “gap repair” methodology in Saccharomyces cerevisiae (Ma et al. ibid) as follows. The yeast-E. coli shuttle vector pRS425::GPM::kivD::ADH which contains the GPM1 promoter (SEQ ID NO:721), kivD coding region from Lactococcus lactis (SEQ D NO:719), and ADH1 terminator (SEQ ID NO:722) (described in U.S. Pat. No. 7,851,188, Example 17) was digested with BbvCI and PacI restriction enzymes to release the kivD coding region. Approximately 1 μg of the remaining vector fragment was transformed into S. cerevisiae strain 13Y4741 along, with 1 mg of sadB PCR product. Transformants were selected on synthetic complete medium lacking leucine. The proper recombination event, generating pRS425::GPM-sadB, was confirmed by PCR using primers N142 and N459 (SEQ ID NOs:729 and 730).


Construction of pdc6::PGPM1-sadB Integration cassette and PDC6 Deletion:


A pdc6:: PGPM1-sadB-ADH/t-URA3r integration cassette was made by joining the GPM-sadB-ADHt segment (SEQ ID NO:723) from pRS425::GPM-sadB (SEQ ID NO: 720) to the URA3r gene from pUC19-URA3r. pUC19-URA3r (SEQ ID NO:724) contains the URA3 marker from pRS426 (ATCC #77107) flanked by 75 by homologous repeat sequences to allow homologous recombination in vivo and removal of the URA3 marker. The two DNA segments were joined by SOE PCR (as described by Horton et al. (1989) Gene 77:61-68) using as template pRS425::GPM-sadB and pUC19-URA3r plasmid DNAs, with Phusion DNA polymerase (New England Biolabs Inc., Beverly, Mass.; catalog no. F-5405) and primers 114117-11A through 114117-11D (SEQ ID NOs:731, 732, 733 and 734), and 114117-13A and 114117-13B (SEQ ID NOs:735 and 736).


The outer primers for the SOE PCR (114117-13A and 114117-13B) contained 5′ and 3′ ˜50 by regions homologous to regions upstream and downstream of the PDC6 promoter and terminator, respectively. The completed cassette PCR fragment was transformed into BY4700 (ATCC #200866) and transformants were maintained on synthetic complete media lacking uracil and supplemented with 2% glucose at 30° C. using standard genetic techniques (Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202). Transformants were screened by PCR using primers 112590-34G and 112590-34H (SEQ ID NOs:737 and 738), and 112590-34F and 112590-49E (SEQ ID NOs: 739 and 740) to verify integration at the PDC6 locus with deletion of the PDC6 coding region. The URA3r marker was recycled by plating on synthetic complete media supplemented with 2% glucose and 5-FOA at 30° C. following standard protocols. Marker removal was confirmed by patching colonies from the 5-FOA plates onto SD-URA media to verify the absence of growth. The resulting identified strain has the genotype: BY4700pdc6::PGpmi-sadB-ADH1t.


Construction of pdc1::PPDC1-ilvD Integration Cassette and PDC1 Deletion:


A pdch:: PPDC1-ilvD-FBA1t-URA3r integration cassette was made by joining the ilvD-FBA1t segment (SEQ ID NO:741) from pLH468 (SEQ ID NO: 1888) to the URA3r gene from pUC19-URA3r by SOE FCR (as described by Horton et al. (1989) Gene 77:61-68) using as template pLH468 and pUC19-UR.\3r plasmid DNAs, with Phusion DNA polymerase (New England Biolabs Inc., Beverly, Mass.; catalog no. F-540S) and primers 114117-27A through 114117-27D (SEQ ID NOs:742, 743, 744 and 745).


The outer primers for the SOE PCR (114117-27A and 114117-27D) contained 5′ and 3′ ˜50 by regions homologous to regions downstream of the PDC1 promoter and downstream of the PDC1 coding sequence. The completed cassette PCR fragment was transformed into BY4700 pdc6::PGPM1-sadB-ADH1t and transformants were maintained on synthetic complete media lacking uracil and supplemented with 2% glucose at 30° C. using standard, genetic techniques (Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202). Transformants were screened by PCR using primers 114117-36D and 135 (SEQ ID NOs 746 and 747), and primers 112590-49E and 112590-30F (SEQ ID NOs 740 and 748) to verify integration at the PDC1 locus with deletion of the PDC1 coding sequence. The URA3r marker was recycled by plating on synthetic complete media supplemented with 2% glucose and 5-FOA at 30° C. following standard protocols. Marker removal was confirmed by patching colonies from the 5-FOA plates onto SD-UR \ media to verify the absence of growth. The resulting identified strain “NYLA67” has the genotype: BY4700 pdc6:: PGPM1-sadB-ADH1t pdc1::_PPDC1-ilvD-FBA1t.


HIS3 Deletion

To delete the endogenous HIS3 coding region, a his 3:URA3r2 cassette was PCR-amplified from URA3r2 template DNA (SEQ ID NO: 749). URA3r2 contains the URA3 marker from pRS426 (ATCC #77107) flanked by 500 by homologous repeat sequences to allow homologous recombination in vivo and removal of the URA3 marker. PCR was done using Phusion DNA polymerase and primers 114117-45A and 114117-45B (SEQ ID NOs:750 and 751) which generated a ˜2.3 kb PCR product. The HIS3 portion of each primer was derived from the 5′ region upstream of the HIS3 promoter and 3′ region downstream of the coding region such that integration of the URA3r2 marker results in replacement of the HIS3 coding region. The PCR product was transformed into NYLA67 using standard genetic techniques (Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202) and transformants were selected on synthetic complete media lacking, uracil and supplemented with 2% glucose at 30° C. Transformants were screened to verify correct integration by replica plating of transformants onto synthetic complete media lacking histidine and supplemented with 2% glucose at 30° C. The URA3r marker was recycled by plating on synthetic complete media supplemented with 2% glucose and 5-FOA at 30° C. following standard protocols. Marker removal was confirmed by patching colonies, from the 5-FOA plates onto SD-URA. media to verify the absence of growth. The resulting identified strain, called NYLA73, has the genotype: BY4700 pdc6 . . . PGPM1/sadB-ADH1t ppc1:: PPDC1-ilvD-FBA1t Δhis3.


Construction of pdc5::kanMX Integration Cassette and PDC5 Deletion:


A pdc5::kanMX4 cassette was PCR-amplified from strain YLR134W chromosomal DNA (ATCC No. 4034091) using Phusion DNA polymetase and primers PDC5::KanMXF and PDC5.:KanMXR (SEQ ID NOs:752 and 753) which generated a ˜2.2 kb PCR product. The PDC5 portion of each primer was derived from the 5′ region upstream of the PDC5 promoter and 3′ region downstream of the coding region such that integration of the kanMX4 marker results in replacement of the PDC5 coding region. The PCR product was transformed into NYLA73 using standard genetic techniques (Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202) and transformants were selected on YP media supplemented with 1% ethanol and geneticin (200 μg/ml) at 30° C. Transformants were screened by PCR to verify correct integration at the PDC locus with replacement of the PDC5 coding region using primers PDC5kofor and N175 (SEQ ID NOs: 754 and 755). The identified correct transformants have the genotype: BY4700 pdc 6:: PGPM1-sadB-ADH1t pdc 1::PPDC1-ilvD-FBA1t Δhis3 pdc5::kanMX4. The strain was named NYLA74.


Deletion of HXK2 (hexokinase II):


A hxk2::URA3r cassette was PCR-amplified from URA3r2 template (described above) using Phusion DNA polymerase and primers 384 and 385 (SEQ ID NOs:756 and 757) which generated a ˜2.3 kb PCR product. The HXK2 portion of each primer was derived from the 5′ region upstream of the HXK2 promoter and 3′ region downstream of the coding region such that integration of the URA3r2 marker results in replacement of the HXK2 coding region. The PCR product was transformed into NYLA73 using standard genetic techniques (Methods in Yeast Genetics, 2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202) and transformants were selected on synthetic complete media lacking uracil and supplemented with 2% glucose at 30° C. Transformants were screened by PCR to verify correct integration at the HXK2 locus with replacement of the HXK2 coding region using primers N869 and N871 (SEQ ID NOs:758 and 759). The URA3r2 marker was recycled by plating on synthetic complete media supplemented with 2% glucose and 5-FOA at 30° C. following standard protocols. Marker removal was confirmed by patching colonies from the 5-FOA plates onto SD-URA media to verify the absence of growth, and by PCR to verify correct marker removal using primers N946 and N947 (SEQ ID NOs:760 and 761). The resulting identified strain named NYLA83 has the genotype: BY4700 pdc6:: PGPM1-sadB-ADH1t pdc1:: PPDC1-ilvD-FBA1t Δhis3 Δhxk2.


Example 12
Construction of Saccharomyces cerevisiae Strain PNY2257

Strain PNY2242 was constructed in several steps from BP913 (described above). First, the native GPD2 gene on Chromsome XV was deleted. The coding region was deleted using CRE-lox mediated marker removal (methodology described above), so the resulting locus contains one loxP site. The sequence of the modified locus is provided as SEQ ID NO 1899 (Upstream region=nt 1-500; loxP site=nt 531-564; Downstream region=nt 616-1115). Second, the native FRA2 gene on Chromosome VII was deleted. Elimination of FRA2 was a scarless deletion of only the coding region. The sequence of the modified locus is provided as SEQ ID NO 1900 (Upstream region=nt 1-501; Downstream region=nt 526-1025). Next, the ADH1 gene on Chromosome XV was deleted along with insertion of a chimeric gene comprised of the UAS(PGK1)—FBA1 promoter and the kivD coding region. The native ADH1 terminator was used to complete the gene. The sequence of the modified locus is provided as SEQ ID No. 1901 (Upstream region nt=1-500; UAS(PGK1)FBA promoter=nt 509-1233; kivD coding region=nt 1242-2888; Downstream region (includes terminator)=nt 2889-3388). Next, a chimeric gene comprised of the FBA1 promoter, the alsS coding region and the CYC1 terminator was integrated into Chromosome XII, upstream of the TRX1 gene. The sequence of the modified locus is provided as SEQ ID No. 1902(Upstream region=nt 1-154; FBA1 promoter=nt 155-802; alsS CDS=nt 810-2525; CYC1 terminator=nt 2534-2788; Downstream region=nt 2790-3015). Next, two copies of a gene encoding, horse liver alcohol dehydrogenase were integrated into Chromsomes VII and XVI. On Chromosome VII, a chimeric gene comprised of the PDC1 promoter, the hADH coding region and the ADH 1 terminator were placed into the fra2A locus (the original deletion of FRA2 is described above). The sequence of the modified locus is provided as SEQ ID No. 1903 (Upstream region=nt 1-300; PDC1 promoter=nt 309-1178; hADH coding region=nt 1179-2306; ADH1 terminator=nt 2315-2630; Downstream region nt 2639-2900). On Chromosome XVI, a chimeric gene comprised of the PDC5 promoter, the hADH coding region and the ADH1 terminator were integrated in, the region formerly occupied by the long term repeat element YPRCdelta15. The sequence of the modified locus is provided as SEQ ID No. 1904 (Upstream region=nt 1-150; PDC5 promoter=nt 159-696; hADH coding region=nt 697-1824; ADH1 terminator=nt 1833-2148: Downstream region=nt 2157-2656). Then the native genes YMR226c and ALD6 were deleted. Elimination of YMR226c was a scarless deletion of only the coding region. The sequence of the modified locus is provided as SEQ ID No. 1905 (Upstream region=nt 1-250; Downstream region=nt 251-451). The ALD6 coding region plus 700 by of upstream sequence were deleted using CRE-lox mediated marker removal, so the resulting locus contains one loxP site. The sequence of the modified locus is provided as SEQ ID No. 1906(Upstream region=nt 1-500; loxP site=nt 551-584; Downstream region=nt 678-1128). The geneticin-selectable phosphoketolase expression vector described in Example 8 was transformed into the strain and confirmed as described above (the locus is depicted in FIG. 6). Finally, plasmids were introduced into the strain for expression of KAR1 (pLH702, plasmid SEQ ID. No. 1907) and DHAD (pYZ067DkivDDhADH, SEQ ID. No. 1908), resulting in the strain named PNY2257. A control strain containing all of the elements above except for the phosphoketolase pathway construct is called PNY2242.


Growth rates were assessed as described in previous examples. Over a 24 hour period, PNY2257 displayed growth rates without ethanol or other two-carbon supplement similar to those growth rates observed for PNY2242 with supplementation.











TABLE 6









HMMER2.0 [2.2g]



NAME XFP_XPK_exp_seqs



LENG 845



ALPH Amino



RF no



CS no



MAP yes



COM /app/public/hmmer/current/bin/hmmbuild XFP_XPK_HMM XFP_XPK_exp_seqs.aln



COM /app/public/hmmer/current/bin/hmmcalibrate - mean 800 XFP_XPK_HMM



NSEQ 8



DATE Fri Dec 4 15:29:49 2009



CKSUM 6589



XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4



NULT −4 −8455



NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644



EVD −614.573792 0.077043

































A
C
D
E
F
G
H
I
K














HMM
m->m
m->i
m->d
i->m
i->i
d->m
d->d
b->m
m->e
L
M
N
P
Q
R
S
T
V
W
Y






−476
*
−1831


1(M)
−579
−646
−1823
−1279
−241
−1949
−816
340
−991
1145
2778
−1234
1286
−828
−1131
−1025
−537
205
−1033
−696
11



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−25
−6428
−7470
−894
−1115
−701
−1378
−476
*


2(W)
−424
−1143
−649
−112
−1118
−1430
−73
−944
1343
−1091
−364
−285
1428
217
174
−480
−363
−730
3083
−756
12



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−25
−6428
−7470
−894
−1115
−701
−1378
*
*


3(Q)
−411
−1812
202
1278
−2120
997
53
−1844
1011
−1806
−940
170
−1370
1483
39
−277
−358
−1435
−1985
−1327
13



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−25
−6428
−7470
−894
−1115
−701
−1378
*
*


4(M)
−554
−1770
−500
1294
−2084
−1496
−45
−1741
1490
−1726
2375
−163
−1571
384
1179
−467
−467
−1393
−1906
−1358
14



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6836
−7878
−894
−1115
−701
−1378
*
*


5(V)
582
−614
−1167
546
−657
−1623
−419
−47
−453
583
188
−696
−1707
−324
−741
−630
773
1221
−1069
−649
15



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6836
−7878
−894
−1115
−701
−1378
*
*


6(M)
−388
−632
233
−716
−648
−1697
−471
1170
−550
−499
1779
218
−1768
−407
−821
−698
1278
799
−1059
−646
16



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


7(D)
54
−2137
2020
1380
−2436
−1385
−196
−2201
126
−2144
−1250
1219
−1583
239
702
−471
−581
−1750
−2320
−1600
17



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


8(Y)
−508
−822
−1280
−702
−673
−1748
−430
832
−332
−675
−3
−776
809
−344
1190
−762
−446
−274
−1076
2458
18



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


9(D)
−469
−1542
1605
42
−1969
−1375
−320
−1627
−36
−1747
−919
−219
1080
65
−522
1153
−479
670
−2048
−1444
19



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


10(S)
−586
−1385
−736
−829
−2829
−1309
−1317
−2735
−1241
−2859
−2047
1835
−1910
−1051
−1602
2833
−881
−1997
−2969
−2410
20



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


11(P)
−526
−1890
−212
1174
−2213
−1420
−120
−1928
1548
−1905
−1024
−80
1551
317
−111
−415
763
−1521
−2093
−1450
21



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


12(D)
1339
−2192
1728
1307
−2485
−1401
−248
−2248
996
−2199
−1317
−28
−1622
180
−495
−529
−648
−1804
−2382
−1658
22



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


13(Y)
984
−983
−1421
−1092
−837
−1586
−819
−1015
−973
−1266
−608
−1029
1341
−855
−1218
−770
−660
−790
−1317
3268
23



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


14(L)
−877
−775
−2589
−1991
1160
−2382
−1170
1029
960
1849
533
−1790
−2366
−1386
−1652
−1454
−810
77
−1124
−783
24



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


15(N)
−730
−2232
123
1772
−2530
−1420
−275
−2293
34
−2239
−1364
1865
1127
1325
−496
−569
−693
−1850
−2418
−1697
25



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


16(S)
−406
−1619
−309
191
−1903
406
−102
−1593
986
−317
−789
1064
−1504
317
−181
1301
−358
−1237
−1903
−1286
26



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7025
−8067
−894
−1115
−701
−1378
*
*


17(M)
−675
−566
−2528
−1928
−364
−2184
2126
218
−1592
743
2772
−1654
−2210
−1313
−1560
−1256
−614
1711
−990
−635
27



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


18(D)
−749
−1575
2786
−299
−2062
−1566
−768
−1153
−635
−1769
−1097
−535
−1902
−450
−1112
−799
731
1070
−2328
−1751
28



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


19(K)
1232
−1674
−736
−295
−2360
−1534
−473
−2014
2326
−2058
−1228
−486
1165
−74
−221
−634
−677
−1596
−2264
−1735
29



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


20(Y)
−2075
−2643
1686
−985
−266
−2358
−869
−2644
−1801
−2467
−2120
−1177
−2732
−1384
−2229
−1931
−2114
−2488
−905
4141
30



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


21(W)
−2473
−2122
−3795
−3534
136
−3469
−1296
−944
−2887
1176
−449
−2889
−3469
−2568
−2708
−2895
−2429
−1317
5419
316
31



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


22(R)
−1335
−1772
−2246
−1513
−2127
−2222
−955
−1242
35
−1675
−1197
−1424
−2426
−659
3303
−1507
−1331
1211
−2220
−1868
32



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


23(A)
1948
−1167
−891
1114
−1539
−1605
−710
−688
−551
−1278
−608
−703
−1861
−451
−928
−724
−609
1295
−1870
−1386
33



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


24(A)
2275
−1339
−993
−558
−2142
−1451
−682
−1733
1352
−1909
−1113
−663
−1785
−334
−564
−587
928
−1336
−2220
−1732
34



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


25(N)
−1216
−1775
−1072
−1034
−1473
−1967
−1153
−1429
−1009
767
−1165
3461
−2339
−1043
−1259
−1335
−1316
−1331
−1982
−1273
35



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


26(Y)
−2131
−1808
−3471
−3249
538
−3299
−814
1516
−2817
−864
−742
−2495
−3305
−2356
−2649
−2520
−2091
−784
−205
4093
36



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


27(L)
−2321
−1872
−4652
−4075
−539
−4266
−3092
2178
−3803
2345
2067
−3915
−3747
−2978
−3487
−3494
−2215
−175
−2130
−2157
37



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


28(S)
−482
−1136
−1858
−1915
−3072
−1315
−1996
−2905
−2045
−3106
−2272
−1475
1651
−1840
−2205
3016
−873
−2011
−3242
−2908
38



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


29(V)
845
−1090
−3817
−3373
−1522
−3377
−2794
2147
−3161
−649
−421
−3056
−3362
−2955
−3177
−2598
−1419
2690
−2606
−2197
39



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


30(G)
−1283
−2067
−837
−965
−3177
3042
−1462
−3119
−1171
−3141
−2429
−1111
−2331
2005
−1448
−1320
−1499
−2538
−3099
−2665
40



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


31(Q)
−974
−2142
−780
−270
−2589
−1791
−328
−2217
1581
−2142
−1329
−487
−1903
3083
262
−876
671
−1860
−2250
−1764
41



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


32(L)
−1375
−1127
−3503
−2949
−697
−3071
−2054
1887
−2629
2035
342
−2656
−2990
−2251
−2534
−2214
1157
428
−1759
−1511
42



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


33(Y)
−1937
−2273
−1926
−1563
−232
−2608
−650
−2164
1538
−2035
−1599
−1501
−2740
−960
−541
−1929
−1851
−2048
−794
4044
43



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


34(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
44



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


35(K)
−565
−1769
−542
1150
−2042
−1544
−133
−1707
1529
−200
1362
−220
−1622
291
1492
−490
−487
−1367
−1948
−1377
45



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


36(D)
−733
−2199
2095
230
−2550
256
−327
−2314
1307
−2269
−1390
−100
−1681
97
−574
1114
−708
−1861
−2454
−1735
46



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


37(N)
−1190
−2662
1381
127
−3153
−1520
−768
−2990
−757
−2945
−2156
3138
−1961
−405
−1416
1061
−1253
−2479
−3137
−2313
47



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


38(P)
−1777
−2801
1868
−561
−3633
−1918
−1524
−3634
−1721
−3620
−2978
−881
3543
−1257
−2350
−1648
−1944
−3091
−3510
−2972
48



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


39(C)
−1617
2940
−3806
−3389
−788
−2963
−2369
−4
−2967
2637
197
−2927
−3094
−2569
−2799
−2284
−1672
−278
−1950
−1680
49



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


40(M)
−1138
−1232
1092
−1546
−625
−2468
−1290
−56
−1453
1974
2682
−1610
−2481
−1228
−1655
−1558
−1079
−283
−1540
−1223
50



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


41(H)
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
51



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


42(K)
784
−2190
−977
−302
−2645
−1847
−256
−2259
2452
−2136
−1309
−504
−1900
1339
1312
−874
−863
−1897
−2202
−1742
52



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


43(E)
−648
−1915
−516
1778
−2222
−1578
−162
−1899
439
163
−1023
−238
−1667
1297
1557
−552
−568
−1536
−2057
−1482
53



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


44(P)
−1091
−2157
−710
−354
−2472
−1828
2409
−2236
1296
−2195
−1418
−568
2737
−72
88
−1007
−1021
−1903
−2268
−1751
54



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


45(L)
−1149
−1110
−2516
−1945
1939
−2543
−1114
−128
1051
1953
309
−1805
−2527
−1346
−1480
−1629
−1078
−306
−1080
−509
55



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


46(T)
−695
−1464
−884
−358
−1467
−1703
−361
−1272
1547
−1430
−690
−543
−1822
−57
−149
−739
2166
−1045
−1654
1676
56



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


47(R)
1626
−2138
−1332
−620
−2693
−1985
−403
−2249
1356
−2167
−1392
−747
−2067
11
2526
−1100
−1051
−1929
−2243
−1870
57



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


48(E)
378
−1941
703
1824
−2252
−1386
−100
−2000
289
−1956
−1046
−24
−1516
349
1172
476
−427
−1561
−2134
−1447
58



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


49(D)
923
−2559
2894
160
−2929
−1519
−635
−2712
−524
−2688
−1882
−189
−1895
1449
−1131
−904
−1111
−2258
−2898
−2120
59



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


50(V)
1091
−1040
−3230
−3029
−1839
−2191
−2521
453
−2807
−1107
−816
−2387
−2674
−2598
−2801
−1506
−1141
3085
−2726
−2349
60



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


51(K)
1016
−1925
−1252
−838
−2825
−1834
−758
−2369
3094
−2408
−1667
−917
−2126
−380
−14
−1094
−1125
−1973
−2524
−2145
61



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


52(H)
−477
−400
−2258
−1664
1050
−1948
2313
1043
−1367
−239
404
−1388
365
−1083
−1355
−1008
−417
915
−715
1744
62



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


53(H)
−1023
−1918
−1234
−509
−2208
−1943
3152
947
1315
−1817
−1055
−662
−1981
64
1825
−993
−895
−1542
−1997
−1584
63



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


54(P)
1113
−913
−1858
−1496
−1358
−1604
−1238
−756
−1296
958
−503
−1306
2592
−1174
−1479
−817
−686
−602
−1858
−1474
64



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


55(I)
846
−1093
−3818
−3374
−1512
−3374
−2792
2798
−3161
−633
−411
−3056
−3360
−2951
−3174
−2596
−1422
2077
−2600
−2195
65



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


56(G)
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
66



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


57(H)
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
67



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


58(W)
−4114
−3274
−4214
−4453
−1722
−3496
−2681
−4109
−4149
−3619
−3615
−4044
−3885
−4032
−3816
−4355
−4248
−4133
6191
−1329
68



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


59(G)
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
69



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


60(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
70



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


61(C)
−859
2735
−3125
−2633
−1043
−2353
−1765
2619
−2314
−561
−137
−2173
−2560
−2046
−2258
−1540
1442
680
−1746
−1384
71



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


62(P)
1490
−891
−2212
−1956
−2077
−1371
−1689
−1274
−1796
−1861
−1203
−1479
2954
−1611
−1936
−653
−684
816
−2478
−2131
72



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


63(G)
−382
−1055
−1768
−1819
−3131
2891
−1951
−2904
−2042
−3099
−2224
−1384
1043
−1771
−2236
1022
−775
−1958
−3301
−2980
73



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


64(Q)
−1532
−1831
−1845
−1487
−1032
−2520
−1248
−707
−794
1863
−184
−1573
−2625
2991
−924
−1768
−1488
−990
−1859
−1397
74



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


65(N)
−1040
−1491
−1193
−1126
−1577
−1932
−1248
1438
−1138
−1433
−1011
3384
−2307
−1120
−1396
−1256
−1150
−579
−2101
−1473
75



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


66(F)
−2629
−2152
−4261
−4009
3717
−3972
−1470
−438
−3655
1364
94
−3253
−3712
−2829
−3311
−3250
−2543
−971
−709
261
76



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


67(I)
−1794
−1354
−4306
−3856
−1267
−3968
−3273
2808
−3666
1262
−89
−3607
−3721
−3300
−3605
−3222
−1758
1880
−2695
−2428
77



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


68(Y)
−1692
−1412
−3458
−3020
258
−3136
−1081
1186
−2630
646
−31
−2459
−3064
−2167
−2476
−2272
−1624
−329
−544
3829
78



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


69(A)
2378
−934
−2147
−1885
−2779
826
−1775
−2485
−1781
−2703
−1842
−1381
−1860
−1577
−1989
1353
1040
−1689
−2983
−2654
79



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


70(H)
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
80



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


71(L)
−2379
−1915
−4669
−4151
−621
−4360
−3248
1934
−3862
2632
553
−4017
−3845
−3099
−3585
−3641
−2289
−24
−2250
−2225
81



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


72(N)
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
82



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


73(R)
−1929
−2343
−2336
−1600
−825
−2607
−677
−2317
45
−2153
−1653
−1506
−2681
−679
3382
−1922
−1792
−2176
−1232
2267
83



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


74(V)
503
−701
−3099
−2506
1199
−2516
−1425
486
−2168
1370
379
−2113
−2515
−1800
−2039
−1621
−848
2021
−1247
−916
84



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


75(I)
−2091
−1746
−3971
−3840
−1676
−3532
−3289
3684
−3581
−659
−693
−3562
−3674
−3445
−3521
−3194
−2146
449
−2877
−2493
85



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


76(K)
1051
−1939
−439
−69
−2445
−1557
−349
−2132
2202
−2116
−1268
1786
−1753
70
−141
−646
−704
−1719
−2284
−1701
86



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


77(K)
−1384
−2800
1892
−69
−3174
−1733
−742
−2966
2973
−2873
−2105
−400
−2087
−365
−775
−1179
−1372
−2535
−2949
−2288
87



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


78(Y)
−2118
−2084
−2592
−2218
1983
−3049
2937
−1851
−1966
−1662
−1339
−1862
−3049
1795
−2085
−2125
−2036
−1809
122
3287
88



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


79(N)
−1107
−2701
1326
223
−2992
1724
−586
−2799
−472
−2733
−1910
2292
−1876
1179
−1098
−894
−1109
−2331
−2923
−2116
89



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


80(L)
−1690
−1806
−2247
−1903
−837
−2782
−1536
−464
−1204
2489
81
−1939
−2835
1986
−1289
−2052
−1642
−850
−1841
−1451
90



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


81(D)
−1292
−2956
2598
202
−3213
−1558
−693
−3045
851
−2958
−2170
2502
−1967
−318
−1262
−1043
−1304
−2569
−3134
−2291
91



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


82(M)
−866
−1094
−2548
−2220
−1176
−1993
−1696
−374
−1810
−470
4232
−1867
−2385
−1729
−1855
−1260
1587
−400
−1960
−1618
92



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


83(F)
−1725
−1400
−3934
−3394
2962
−3436
−2055
325
−3076
1369
437
−3005
−3243
−2505
−2863
−2595
−1653
1644
−1488
−995
93



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


84(Y)
−2852
−2251
−3961
−3935
2281
−3735
−476
168
−3538
−1379
−1299
−2630
−3641
−2627
−3134
−2925
−2756
−1840
233
4154
94



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


85(I)
−1759
−1305
−4328
−3965
−1743
−4047
−3735
3086
−3833
−588
−522
−3726
−3872
−3681
−3903
−3364
−1752
2354
−3250
−2813
95



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


86(M)
−700
−1108
−1144
2046
−1106
−1894
−710
−177
−572
−648
2421
−857
−1997
−497
−908
−934
−657
1171
−1602
−1155
96



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


87(G)
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
97



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


88(P)
−526
−1169
−1848
−1957
−3095
−1343
−2052
−2946
−2123
−3152
−2333
−1513
3407
−1917
−2261
1632
−922
−2053
−3267
−2938
98



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


89(G)
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
99



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


90(H)
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
100



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


91(G)
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
101



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


92(H)
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
102



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


93(G)
1432
−1081
−2340
−2519
−3309
3113
−2408
−3041
−2687
−3327
−2482
−1740
−2062
−2353
−2689
−702
−903
−2058
−3476
−3301
103



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


94(Q)
−1326
−2187
−825
−789
−2878
−1884
−1131
−2784
−631
−2752
−2063
−977
2646
3194
−881
−1313
−1428
−2357
−2787
−2310
104



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


95(V)
1470
−815
−2475
−2234
−1874
1135
−1780
−892
−2064
−1657
−1016
−1624
−2020
−1814
−2121
−717
−687
2477
−2329
−1999
105



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


96(M)
−883
−899
−2454
−1951
−595
991
−1285
−31
−1626
1077
3599
−1754
−2362
−1420
−1669
−1376
−878
−131
−1359
−1024
106



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


97(V)
−1205
−914
−3493
−2955
−1023
−3025
−2085
1649
−2672
917
−32
−2617
−2982
−2375
−2606
−2169
1373
2267
−1903
−1552
107



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


98(S)
1719
−1145
−1296
−1130
−2738
−1260
−1357
−2461
−1234
−2624
−1769
1171
−1844
−1070
1576
2383
−686
−1744
−2874
−2423
108



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


99(N)
−809
−2086
−98
33
−2593
−1494
−488
−2361
−147
−2346
−1503
2681
−1787
1970
−607
984
−825
−1908
−2521
−1851
109



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


100(S)
878
−890
−1818
−1458
−1679
−1418
−1269
−1166
−1293
665
−837
−1224
−1904
−1156
−1509
2469
−610
−869
−2068
−1669
110



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


101(Y
−3588
−2709
−4120
−4323
1034
−3994
−419
−2613
−3834
−2031
−2055
−2741
−3905
−2826
−3349
−3280
−3472
−2736
3673
4312
111



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


102(L)
−2496
−2027
−4721
−4202
−560
−4432
−3262
1210
−3887
2852
611
−4095
−3876
−3086
−3587
−3729
−2397
−232
−2211
−2201
112



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


103(D
−1791
−3663
3414
1659
−3853
−1647
−1043
−3807
−1343
−3679
−3054
−251
−2201
−725
−2227
−1438
−1872
−3282
−3836
−2831
113



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


104(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
114



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


105(S)
−342
−976
−2177
−2128
−2921
−1229
−2008
−2606
−2073
−2888
−2058
−1512
−1936
−1871
−2189
2669
2327
−1779
−3151
−2842
115



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


106(Y
−2652
−2206
−3673
−3556
726
−3580
−692
−1359
−3066
982
−936
−2620
−3538
−2500
−2832
−2837
−2587
−1595
−24
4185
116



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


107(T)
−502
−1110
−2201
−2282
−3095
1340
−2204
−2779
−2341
−3075
−2268
−1660
−2066
−2111
−2412
−729
3346
−1945
−3276
−3043
117



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


108(E)
−1163
−2811
1893
2460
−3072
−1535
−594
−2884
1337
−2797
−1978
−140
−1898
−203
−1072
−933
−1159
−2412
−2972
−2157
118



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


109(I)
561
−338
−2506
−1896
1485
−1980
−829
1917
−1557
−155
470
−1524
−2033
−1236
−1465
−1055
777
309
−776
1058
119



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


110(Y
−3611
−2705
−4166
−4409
2531
−4042
−397
−2533
−3989
−1938
−1983
−2747
−3929
−2851
−3444
−3294
−3491
−2684
345
4289
120



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


111(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
121



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


112(E)
−785
−2281
−2
1960
−2596
−1496
−297
−2348
660
−2275
−1399
1934
−1705
1721
−381
−626
−738
−1906
−2432
−1737
122



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


113(I)
−1722
−1438
−3424
−3080
131
−3150
−1169
2917
−2681
−503
−344
−2513
−3144
−2305
−2554
−2347
−1683
−39
−645
2880
123



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


114(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
124



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


115(Q
−1609
−2594
−1354
−790
−3182
−2213
−519
−2737
2527
−2514
−1787
−903
−2297
3031
525
−1483
−1437
−2432
−2460
−2150
125



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


116(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
126



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


117(E)
−495
−1766
−320
2020
−2044
−1449
−133
−1708
1298
−1759
−898
−124
−1562
295
−155
−410
632
108
−1998
−1374
127



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


118(E)
1485
−2051
−202
1709
−2406
−1511
−270
−2112
1557
−2089
−1228
−183
−1688
156
−234
−586
−657
−1706
−2272
−1633
128



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


119(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
129



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


120(M
−2568
−2113
−4714
−4154
−462
−4418
−3155
99
−3799
2469
3538
−4062
−3833
−2972
−3479
−3691
−2450
−587
−2108
−2139
130



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


121(Q
447
−1907
−448
−115
−2176
−1605
2296
−1980
143
−2000
−1194
−367
−1799
3046
−194
−715
−752
−1626
−2150
−1543
131



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


122(K
−1448
−2495
−1440
−709
−3095
−2161
−406
−2616
2874
−2399
−1636
1544
−2197
22
1483
−1326
−1270
−2295
−2364
−2043
132



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


123(L)
−2430
−1987
−4659
−4071
2109
−4260
−2828
67
−3779
2463
2382
−3869
−3717
−2880
−3418
−3473
−2306
−623
−1874
−1724
133



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


124(F)
−2144
−1762
−4034
−3706
3638
−3656
−1587
1655
−3381
102
23
−3073
−3507
−2734
−3134
−2896
−2093
−241
−906
16
134



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


125(K
−1297
−2351
−1405
−643
−2938
−2076
−377
−2482
2603
−2306
−1527
−768
−2120
51
1973
899
−1147
−2149
−2307
−1957
135



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


126(Q
−753
−1991
−692
−91
−2345
−1670
1700
−2009
519
−1957
−1111
−334
−1745
2315
1728
−656
1061
−1645
−2095
−1558
136



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


127(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
137



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


128(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
138



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


129(F)
−1908
−1643
−3261
−2963
3263
−3050
−509
−1219
−2636
−1197
−851
−2199
−3071
−2085
−2476
−2189
1297
−1211
77
2811
139



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


130(P)
−540
−1180
−1850
−1974
−3103
−1352
−2072
−2962
−2149
−3170
−2355
−1527
3509
−1943
−2279
1382
−938
−2068
−3276
−2949
140



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


131(G
1432
−1081
−2340
−2519
−3309
3113
−2408
−3041
−2687
−3327
−2482
−1740
−2062
−2353
−2689
−702
−903
−2058
−3476
−3301
141



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


132(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
142



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


133(V
−1322
−992
−3697
−3181
−1158
−3230
−2353
2069
−2914
914
−126
−2840
−3160
−2624
−2849
−2396
641
2352
−2122
−1765
143



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


134(P)
2498
−1088
−2250
−2290
−3023
−1338
−2177
−2668
−2306
−2970
−2190
−1656
2760
−2080
−2382
−710
−882
−1878
−3231
−2984
144



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


135(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
145



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


136(H
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
146



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


137(A
1947
−745
−2823
−2285
1846
−2348
−1328
1572
−1988
−154
207
−1942
−2439
−1677
−1944
−1473
−836
359
−1231
−781
147



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


138(A
2029
−2389
1954
92
−2936
−1492
−703
−2716
−623
−2715
−1906
1712
−1898
−331
−1237
−881
−1089
−2229
−2931
−2168
148



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


139(P)
1935
−1116
−2232
−2301
−3058
−1358
−2206
−2705
−2336
−3009
−2238
−1674
3272
−2114
−2406
−739
−914
−1913
−3260
−3019
149



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


140(E)
−1349
−2706
−162
2827
−3109
−1785
−624
−2820
1799
−2704
−1931
−437
−2073
−230
−334
−1160
−1298
−2421
−2774
−2189
150



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


141(T)
−645
−1000
−2671
−2474
−1925
−1699
−2057
−385
−2200
−1416
−1020
−1885
−2274
−2057
−2247
−1011
3256
1160
−2570
−2205
151



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


142(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
152



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


143(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
153



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


144(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
154



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


145(S)
1426
−949
−2380
−2419
−3107
−1201
−2226
−2819
−2411
−3105
−2246
−1618
−1944
−2131
−2456
3026
−742
−1874
−3336
−3080
155



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


146(I)
−2091
−1746
−3971
−3840
−1676
−3532
−3289
3684
−3581
−659
−693
−3562
−3674
−3445
−3521
−3194
−2146
449
−2877
−2493
156



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


147(H
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
157



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


148(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
158



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


149(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
159



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


150(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
160



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


151(E)
669
−2323
−22
3133
−3044
−1600
−954
−2706
−853
−2824
−2095
−473
−2059
−614
−1376
−1068
−1279
−2254
−3056
−2376
161



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


152(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
162



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


153(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
163



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


154(Y
−3482
−2868
−3701
−3919
238
−3552
−1112
−3000
−3638
−2516
−2526
−3027
−3772
−3101
−3341
−3418
−3527
−3071
−441
4711
164



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


155(S)
2313
−937
−2414
−2419
−3070
−1197
−2205
−2774
−2385
−3059
−2200
−1614
−1936
−2106
−2436
2518
−728
−1846
−3302
−3046
165



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


156(L)
−2364
−1901
−4660
−4144
−630
−4349
−3246
2015
−3856
2597
544
−4006
−3841
−3101
−3583
−3629
−2275
2
−2255
−2227
166



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


157(S)
−643
−1153
−1942
−1769
−1726
−1574
−1553
−1424
−1560
381
−1167
−1475
−2124
−1504
−1711
2968
−911
−1168
−2234
−1683
167



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


158(H
−1208
−1519
−1818
−1600
−796
−2137
4436
−1042
−1134
−1422
−996
−1514
−2459
−1277
−1260
−1442
−1296
1290
−1367
−527
168



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


159(G
1625
−934
−2312
−2287
−3120
2515
−2142
−2866
−2304
−3096
−2206
−1553
−1910
−2010
−2401
1405
−705
−1884
−3324
−3069
169



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


160(Y
553
−516
−2670
−2093
1648
−2153
−831
125
−1753
−275
301
−1673
−2204
−1407
−1649
−1238
−604
1878
−648
2121
170



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


161(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
171



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


162(A
3438
−1472
−2846
−3040
−3287
−1726
−2735
−2840
−3028
−3257
−2662
−2236
−2447
−2798
−2944
−1216
−1387
−2183
−3405
−3320
172



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


163(I)
−1759
−1304
−4329
−3967
−1748
−4050
−3739
3054
−3836
−593
−525
−3728
−3874
−3686
−3907
−3367
−1751
2401
−3255
−2817
173



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


164(M
−2389
−1957
−4609
−4018
1345
−4211
−2821
78
−3724
2123
3673
−3822
−3688
−2852
−3378
−3418
−2267
−598
−1888
−1767
174



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


165(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
175



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


166(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
176



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


167(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
177



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


168(D
−1220
−2896
2608
1191
−3149
−1530
−651
−2978
−602
−2895
−2089
1257
−1925
−269
−1273
1237
−1229
−2498
−3083
−2233
178



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


169(L)
−1037
−975
−2376
275
−735
−2518
−1412
367
−1669
2017
219
−1835
−2540
−1484
−1766
−1625
−988
1640
−1541
−1207
179



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


170(I)
−1918
−1539
−4076
−3666
2083
−3676
−2142
3117
−3379
145
94
−3221
−3504
−2843
−3198
−2911
−1872
334
−1508
−773
180



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


171(V
2267
−970
−2988
−2782
−1867
−1873
−2265
74
−2556
−1277
−903
−2107
−2419
−2332
−2560
−1182
−982
2421
−2604
−2249
181



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


172(A
2401
−935
−2092
−1865
−2596
−1235
−1739
−2211
−1758
−2497
−1687
−1386
1817
−1571
−1946
−528
1549
−1545
−2846
−2512
182



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


173(C
152
3737
−3346
−3008
−1521
−2075
−2187
405
−2700
−983
−575
−2269
−2516
−2413
−2614
−1349
−959
2580
−2260
−1908
183



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


174(V
−1189
−1030
−2667
1142
−1294
−2870
−1904
1998
−2055
−599
−276
−2175
−2880
−1914
−2228
−1998
−1157
2462
−2134
−1721
184



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


175(V
−1753
−1297
−4327
−3965
−1769
−4049
−3747
2530
−3837
−621
−545
−3725
−3875
−3695
−3913
−3366
−1745
2911
−3274
−2827
185



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


176(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
186



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


177(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
187



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


178(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
188



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


179(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
189



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


180(A
3124
−934
−2490
−2562
−3082
−1203
−2297
−2766
−2535
−3081
−2234
−1670
−1954
−2236
−2534
920
−746
−1844
−3332
−3091
190



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


181(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
191



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


182(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
192



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


183(G
1080
−1108
−2311
−2509
−3336
3223
−2424
−3074
−2706
−3361
−2521
−1751
−2082
−2372
−2708
−729
−932
−2089
−3493
−3324
193



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


184(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
194



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


185(L)
−1369
−1384
−3092
−2737
−825
−2649
−2073
−44
−2334
2571
187
−2437
−2847
−2125
−2306
−1915
1278
−290
−1941
−1678
195



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


186(A
2912
−1000
−2422
−2157
−1636
−1634
−1753
−816
−1876
−1128
1988
−1694
−2169
−1741
−1956
−923
−861
−675
−2256
−1899
196



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


187(T)
973
−953
−2483
−2484
−2838
−1268
−2195
−2209
−2352
−2704
−1971
−1675
−1991
−2131
−2373
−617
3427
−1554
−3148
−2888
197



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


188(S)
−437
−1083
−2006
−2144
−3262
1583
−2197
−3097
−2380
−3307
−2434
−1562
−2008
−2077
−2485
2965
−857
−2074
−3426
−3160
198



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


189(W
−4114
−3274
−4214
−4453
−1722
−3496
−2681
−4109
−4149
−3619
−3615
−4044
−3885
−4032
−3816
−4355
−4248
−4133
6191
−1329
199



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


190(H
−1323
−1832
−1402
−994
2149
−2268
3183
−1595
−684
−1541
−1023
−1062
−2358
2478
−951
−1335
−1257
−1461
−476
569
200



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


191(S)
1326
−873
−2274
−2002
−2263
−1287
−1735
−1644
−1844
−2124
−1388
−1463
−1915
−1637
−1990
2623
−646
880
−2605
−2269
201



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


192(N
−573
−1630
864
62
−1860
−1478
−310
843
−39
−1634
−837
2440
−1658
65
−526
−537
638
−1154
−1980
−1380
202



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


193(K
−1324
−1987
−1657
−1059
−2437
−2134
−750
−1568
3217
−1940
−1374
−1107
−2304
−391
150
−1379
−1277
548
−2320
−1952
203



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


194(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
204



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


195(F)
−2721
−2160
−3990
−3906
3360
−3716
−572
−1433
−3510
1177
−922
−2674
−3597
−2614
−3108
−2899
−2622
−1671
137
2722
205



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


196(I)
−1769
−1321
−4308
−3881
−1430
−3998
−3403
2941
−3713
744
−240
−3637
−3769
−3419
−3696
−3267
−1740
2073
−2864
−2543
206



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


197(N
−1660
−3287
1909
69
−3610
−1646
−1046
−3616
−1275
−3528
−2867
3539
−2186
−731
−2069
−1373
−1757
−3086
−3624
−2697
207



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


198(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
208



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


199(R
780
−954
−1042
−463
−1021
−1681
−388
1232
838
−838
−132
−612
−1756
−173
1322
−665
−418
574
−1339
−893
209



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


200(N
−622
−2053
−110
1285
−2366
−1450
−199
−2102
1009
−2062
−1176
2124
−1614
238
−276
−491
1247
−1677
−2241
−1566
210



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


201(D
−1369
−2674
3374
−58
−3453
−1571
−1095
−3380
−1289
−3368
−2659
−394
−2124
−784
−2016
1149
−1529
−2785
−3505
−2662
211



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


202(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
212



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


203(A
2246
−790
−2416
−1988
−1270
−1821
−1490
1726
−1755
−905
−386
−1651
−2194
−1557
−1854
−1027
949
146
−1839
−1474
213



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


204(V
−1771
−1603
−3750
−3689
−2037
−3050
−3231
403
−3479
−1154
−1076
−3246
−3399
−3383
−3437
−2628
−1917
3536
−3074
−2677
214



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


205(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
215



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


206(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
216



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


207(I)
−2091
−1746
−3971
−3840
−1676
−3532
−3289
3684
−3581
−659
−693
−3562
−3674
−3445
−3521
−3194
−2146
449
−2877
−2493
217



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


208(L)
−2475
−2006
−4716
−4195
−570
−4423
−3262
1353
−3887
2819
603
−4084
−3871
−3089
−3590
−3715
−2377
−194
−2218
−2208
218



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


209(H
−1047
−2367
−130
−12
−2443
−1653
3422
−2425
11
−2366
−1567
2352
−1902
1348
−351
−897
−1003
−2040
−2389
−1693
219



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


210(L)
−1968
−1597
−4244
−3772
−812
−3841
−2955
582
−3473
2391
322
−3529
−3600
−2960
−3315
−3097
−1926
2070
−2269
−2100
220



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


211(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
221



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


212(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
222



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


213(F)
−3581
−2688
−4165
−4401
3865
−4034
−401
−2491
−3981
−1903
−1944
−2746
−3921
−2847
−3438
−3284
−3462
−2647
341
3054
223



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


214(K
−2620
−2961
−2461
−2046
−3743
−2791
−1570
−3603
3784
−3387
−2839
−2048
−3039
−1260
−465
−2604
−2536
−3331
−3001
−2988
224



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


215(I)
−2091
−1746
−3971
−3840
−1676
−3532
−3289
3684
−3581
−659
−693
−3562
−3674
−3445
−3521
−3194
−2146
449
−2877
−2493
225



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


216(A
2478
−935
−2423
−2429
−3066
−1197
−2208
−2769
−2392
−3055
−2196
−1617
−1936
−2112
−2440
2351
−728
−1843
−3300
−3045
226



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


217(N
−1558
−3098
310
1592
−3431
−1657
−945
−3340
−959
−3271
−2572
3495
−2146
−611
−1578
−1301
−1621
−2860
−3383
−2547
227



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


218(P)
−1664
−2231
−2030
−1695
−3192
−2240
−1444
−3029
−556
−2975
−2319
−1668
3549
−1149
1833
−1745
−1774
−2613
−2848
−2650
228



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


219(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
229



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


220(I)
−1764
−1326
−4294
−3932
−1658
−3987
−3644
3354
−3776
−498
−454
−3684
−3833
−3599
−3826
−3303
−1760
1799
−3153
−2738
230



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


221(F)
−2604
−2127
−4552
−4135
2542
−4236
−2203
−115
−3820
2542
500
−3692
−3785
−2927
−3457
−3508
−2493
−769
−1337
−643
231



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


222(S)
846
−947
−2243
−2217
−3137
1905
−2112
−2888
−2254
−3112
−2218
−1528
−1909
−1968
−2369
2545
−708
−1899
−3331
−3067
232



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


223(R
−1454
3144
−2763
−2070
−2728
−2193
−1414
−2383
−460
−2518
−1907
−1827
−2567
−1160
3510
−1626
−1582
−2068
−2596
−2366
233



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


224(M
−434
−912
−967
653
−960
−1640
−368
1179
682
−787
2592
−569
−1718
−161
−575
−617
633
−341
−1294
−841
234



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


225(S)
−1102
−2199
1764
−235
−3206
−1518
−1117
−3089
−1203
−3129
−2368
−513
−2060
−804
−1816
2841
−1292
−2470
−3271
−2541
235



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


226(D
−1724
−3448
3411
103
−3726
−1642
−1058
−3734
−1342
−3628
−2991
1884
−2196
−745
−2191
−1408
−1822
−3197
−3743
−2768
236



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


227(E)
391
−2060
−303
2460
−2453
−1565
−312
−2145
213
−2119
−1272
−258
−1745
110
1394
−657
−720
−1747
−2290
−1683
237



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


228(E)
−843
−2213
−113
2617
−2562
−1553
−357
−2268
1012
−2237
−1392
−212
−1769
60
−324
−706
776
−1863
−2412
−1764
238



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


229(L)
−2324
−1864
−4634
−4121
−657
−4319
−3239
2224
−3838
2493
517
−3975
−3830
−3106
−3578
−3596
−2239
73
−2272
−2231
239



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


230(H
−505
−1236
−775
−228
−1373
−1593
2318
−963
872
−1178
−425
−432
−1693
14
−290
−578
1743
825
−1578
−1083
240



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


231(C
−561
2259
1449
1476
−2294
−1417
−164
−2034
1472
−2003
−1111
−57
−1572
275
−300
−433
−510
−1609
−2194
−1510
241



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


232(Y
−2882
−2267
−4018
−3995
2580
−3778
−511
−1658
−3593
923
−1152
−2675
−3663
−2654
−3169
−2969
−2779
−1865
205
3751
242



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


233(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
243



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


234(H
−559
−1906
−319
1741
−2211
−1478
2491
−1923
355
−1899
−1021
−132
−1591
303
791
−452
1019
−1527
−2083
−1454
244



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


235(G
−456
−1098
−1993
−2173
−3296
2968
−2244
−3141
−2461
−3355
−2485
−1583
−2025
−2139
−2549
1723
−881
−2104
−3461
−3203
245



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


236(M
−1532
−1301
−3405
−2864
−57
−3050
−1438
−56
−2477
1096
3967
−2484
−2939
−2042
−2342
−2169
−1455
−370
−989
2079
246



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


237(G
−1038
−1765
−926
−1191
−3372
3233
−1826
−3379
−1926
−3481
−2725
1285
−2271
−1628
−2264
−1145
−1377
−2554
−3401
−2980
247



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


238(W
−3553
−2695
−4100
−4288
1022
−3972
−428
−2592
−3792
−2021
−2038
−2737
−3890
−2815
−3323
−3265
−3440
−2712
4944
3677
248



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


239(H
−693
−2112
−279
1837
−2456
−1534
2434
−2176
1108
−2098
−1217
−167
−1666
1691
−39
−560
−624
−1753
−2235
−1604
249



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


240(P)
−853
−1252
−2460
−2393
−2139
−1784
−2109
−974
−2210
−1748
−1364
−1924
3453
−2108
−2271
−1167
−1152
1448
−2678
−2307
250



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−812
−7108
−1241
−894
−1115
−701
−1378
*
*


241(Y
−906
−1365
−1255
−688
−4
−1806
−48
−1150
314
−1157
−604
−702
−1897
−107
2106
−965
−822
−1011
−491
2916
251



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−27
−6323
−7365
−894
−1115
−292
−2448
*
*


242(Y
−476
−775
−1198
1077
−755
−1738
−505
1586
−528
−626
43
−777
−1822
−410
−817
−751
649
−143
−1165
1945
252



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


243(F)
−1857
−1592
−3755
−3460
3638
−3255
−1454
−16
−3144
−214
−206
−2800
−3287
−2604
−2951
−2505
−1863
1480
−853
99
253



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


244(V
−1203
−1434
−1278
1166
−1756
−2236
−1499
75
−1412
−1192
−819
−1390
−2525
−1332
−1696
−1574
−1248
2962
−2384
−1907
254



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


245(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
255



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


246(E)
1028
−2230
1464
1545
−2560
−1444
−343
−2324
−77
−2285
−1411
−93
−1690
78
−635
1220
−730
−1876
−2478
−1750
256



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


247(G
802
−892
−1664
−1291
−1774
2148
−1181
−1363
−1177
617
−906
−1109
−1839
−1027
−1436
836
−560
−1000
−2098
−1700
257



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


248(F)
−737
−1710
1482
1223
2205
−1628
−301
−1513
−202
−1603
−865
−339
−1790
−72
−681
−695
−688
−1259
−1410
1271
258



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


249(D
−1662
−3241
3494
12
−3550
−1708
−991
−3449
907
−3360
−2675
−360
−2197
−658
−1631
−1384
−1715
−2975
−3451
−2636
259



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


250(K
374
−1824
783
927
−2121
−1368
−40
525
1057
−1830
−920
824
249
411
−141
−290
−342
−1433
−2020
−1349
260



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


251(E)
424
−2247
1450
1906
−2557
209
−314
−2325
−37
−2276
−1395
−75
659
111
−595
−584
−710
−1874
−2461
−1729
261



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−570
−7108
−1648
−894
−1115
−701
−1378
*
*


252(D
−1129
−2752
2889
513
−3030
−1216
−532
−2959
−669
−2896
−2188
2229
−1714
−198
−1413
−860
−1196
−2465
−3041
−2140
262



−149
−500
233
43
−379
399
105
−627
212
−466
−721
275
394
45
96
359
117
−370
−295
−248



−754
−2619
−2035
−2949
−200
−992
−1008
*
*


253(H
−696
−961
−1261
−873
−178
−1785
3652
1381
−427
−516
−92
−889
−1934
−521
−602
−945
−673
−203
−756
22
273



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−25
−6457
−7499
−894
−1115
−1666
−546
*
*


254(M
−1561
−1309
−3310
−2786
−99
−3163
−1984
569
−2364
1604
3948
−2656
−2936
−1983
−2284
−2361
−1493
116
−1472
−1272
274



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−25
−6457
−7499
−894
−1115
−777
−1263
*
*


255(K
−475
−1891
−137
1054
−2221
−1359
−30
−1946
1632
−1890
−999
2
−1483
1488
30
1135
−411
−1523
−2049
−1403
275



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


256(V
−1531
−1105
−4020
−3576
−1227
−3695
−3034
2323
−3387
793
−62
−3320
−3500
−3101
−3368
−2941
−1501
2608
−2576
−2243
276



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


257(H
−1244
−2186
−246
−319
−1501
−1694
4400
−2492
−417
−2436
−1795
1396
−2075
−501
−711
−1152
−1294
−2140
−1842
−960
277



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


258(R
−675
−1969
−242
1673
−2340
−1479
−164
−2032
420
−1985
−1141
−153
1471
261
1760
−560
−613
−1643
−2136
−1550
278



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−453
−1893
*
*


259(E)
377
−1835
1113
1421
−2128
−1382
−69
−1857
329
−1840
−939
−29
−1493
377
1329
−322
−374
19
−2039
−1371
279



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


260(M
−2315
−1899
−4526
−3941
2102
−4105
−2681
70
−3638
1954
1509
−3704
−3629
−2798
−3304
−3301
−2198
−582
−1802
−1604
280



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


261(A
2818
−1372
−1321
−1214
−2015
−1557
2623
−2096
−1108
−2284
−1581
−1154
−2074
−1121
−1340
−881
−964
−1649
−2321
−1711
281



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


262(K
−793
−2177
−220
1469
−2548
−1561
−289
−2263
2325
−2196
−1334
−214
−1738
141
−150
898
−737
−1844
−2341
−1711
282



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


263(A
1931
−776
−2058
−1612
−1165
−1647
−1206
−433
−1382
945
−310
−1370
−2014
−1216
−1530
−832
1640
−293
−1672
−1305
283



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


264(M
−1519
−1247
−3637
−3077
1725
−3081
−1493
−37
−2718
1089
3901
−2600
−2950
−2153
−2487
−2201
−1437
−379
1806
−356
284



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


265(D
−1378
−3094
2801
1763
−3349
−1556
−771
−3202
−821
−3114
−2349
−164
−2006
−409
−1544
1050
−1411
−2709
−3307
−2411
285



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


266(W
−426
−1591
−384
832
−1812
−1435
−111
−1488
966
−1565
−720
−131
−1532
300
−184
757
1423
−1162
2539
−1242
286



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


267(V
1401
−623
−2516
−1981
−808
−2086
−1255
1461
−1718
−518
36
−1694
−2253
−1472
−1752
293
−658
1939
−1375
−1008
287



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


268(W
−857
−644
−3104
−2515
1895
−2435
−1142
1168
−2142
−39
357
−2021
−2440
−1737
−1961
−1534
−797
2161
2512
−335
288



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


269(E)
−1213
−2845
1928
2618
−3115
−1534
−660
−2926
−607
−2864
−2064
−150
−1929
−281
−1267
−980
833
−2457
−3061
−2225
289



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


270(E)
−1357
−2719
−146
2868
−3119
−1782
−634
−2833
1719
−2718
−1948
−434
−2077
−242
−361
−1166
−1309
−2433
−2790
−2201
290



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


271(I)
−2091
−1746
−3971
−3840
−1676
−3532
−3289
3684
−3581
−659
−693
−3562
−3674
−3445
−3521
−3194
−2146
449
−2877
−2493
291



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


272(C
−1095
2832
−1254
−506
−2502
−1969
1775
−2085
2238
−2024
−1241
−660
−2004
96
1706
−1025
−959
−1790
−2126
−1723
292



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


273(D
1682
−2499
2383
115
−3011
−1503
−717
−2803
−660
−2789
−1985
1727
−1918
−347
−1292
−922
−1145
−2313
−2999
−2214
293



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


274(I)
−2091
−1746
−3971
−3840
−1676
−3532
−3289
3684
−3581
−659
−693
−3562
−3674
−3445
−3521
−3194
−2146
449
−2877
−2493
294



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


275(Q
−1627
−2584
−1823
−913
−3248
−2312
−430
−2707
1993
−2449
−1710
−969
−2306
3139
1660
−1504
−1407
−2413
−2377
−2126
295



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


276(K
949
−2020
−181
1024
−2347
−1460
−176
−2077
2067
−2031
−1143
996
−1606
264
−187
−477
−547
−1653
−2203
−1543
296



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


277(H
1280
−1815
−274
261
−2114
−1392
1493
−1840
1076
−1818
−916
946
−1489
1230
−102
−316
245
−1427
−2010
−1354
297



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


278(A
3438
−1472
−2846
−3040
−3287
−1726
−2735
−2840
−3028
−3257
−2662
−2236
−2447
−2798
−2944
−1216
−1387
−2183
−3405
−3320
298



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


279(R
−836
−1922
−846
−238
−2255
−1752
−261
−1878
494
−1879
−1072
484
−1828
1594
2601
−770
−741
369
−2053
−1564
299



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


280(E)
−531
−1917
−152
1776
−2240
−1412
−166
−1971
212
−1955
−1067
−76
−1565
1351
−287
854
1223
−1553
−2155
−1485
300



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


281(N
−756
−2277
1639
264
−2584
735
−320
−2357
−45
−2301
−1420
1764
−1681
1399
−604
499
−725
−1902
−2482
−1746
301



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1243
−7108
−811
−894
−1115
−701
−1378
*
*


282(T)
391
−254
−749
−541
−1519
−523
−582
−1080
−464
−1400
−646
−345
−1090
−368
−718
1584
1914
−580
−1775
−1351
302



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−36
−5902
−6944
−894
−1115
−2014
−410
*
*


283(N
1419
−678
−16
71
−1606
−696
−281
−1227
−86
−1488
−735
2022
−1167
9
−450
17
−79
−791
−1790
−1251
303



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−36
−5902
−6944
−894
−1115
−655
−1455
*
*


284(N
−690
−2252
1313
1338
−2538
−1300
−208
−2319
1491
−2250
−1381
1988
−1564
211
−512
−509
−660
−1861
−2424
−1672
304



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−20
−6788
−7830
−894
−1115
−421
−1984
*
*


285(D
−1304
−3019
2680
1285
−3255
−1544
−705
−3097
−703
−3006
−2219
1388
1348
−331
−1399
−1044
−1321
−2611
−3195
−2320
305



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


286(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
306



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


287(M
1003
−593
321
−1362
−615
−2007
−849
1868
−1149
−373
2071
−1286
−2064
−949
−1296
−1049
−514
769
−1128
−747
307



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


288(M
−426
−945
−995
−468
−1082
−1580
1667
−681
−299
−938
2081
−605
−1724
−229
−641
445
2054
−498
−1403
−940
308



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


289(R
−1619
−2365
−2075
−1064
−2835
−2363
−469
−2305
1823
763
−1488
−1073
−2345
−67
2817
−1563
−1402
−2096
−2243
−1997
309



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


290(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
310



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


291(Q
−802
−740
−2393
−1814
1799
−2272
−1035
123
−1529
790
401
−1638
−2287
2034
−1573
−1335
−740
1456
−1046
−619
311



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


292(W
−3500
−2678
−4063
−4226
995
−3934
−449
−2563
−3717
−2009
−2016
−2732
−3866
−2798
−3276
−3242
−3394
−2681
5405
3043
312



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


293(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
313



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


294(M
−2406
−2296
−3638
−3594
−1525
−3105
−2824
−1047
−3121
−596
5043
−3293
−3425
−3046
−2996
−2911
−2552
−1398
−2513
−2207
314



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


295(I)
−2034
−1595
−4395
−3950
−974
−4099
−3249
3261
−3681
1202
174
−3748
−3784
−3212
−3556
−3388
−1988
627
−2497
−2285
315



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


296(I)
−1757
−1299
−4333
−3971
−1766
−4057
−3755
2831
−3844
−614
−541
−3733
−3880
−3700
−3920
−3375
−1749
2665
−3275
−2830
316



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


297(F)
−2590
−2117
−4458
−4084
3096
−4144
−1957
−191
−3757
2203
405
−3544
−3752
−2887
−3401
−3410
−2485
−812
−1135
−337
317



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


298(R
−2957
−3022
−3318
−2735
−3796
−2998
−1968
−3912
−846
−3631
−3157
−2611
−3280
−1724
4056
−3026
−2913
−3650
−3096
−3185
318



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


299(T)
2050
−905
−2305
−2099
−2720
−1206
−1895
−2371
−1974
−2648
−1815
−1479
−1888
−1753
−2118
1016
2546
−1624
−2968
−2665
319



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


300(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
320



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


301(K
−2620
−2961
−2461
−2046
−3743
−2791
−1570
−3603
3784
−3387
−2839
−2048
−3039
−1260
−465
−2604
−2536
−3331
−3001
−2988
321



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


302(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
322



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


303(W
−4114
−3274
−4214
−4453
−1722
−3496
−2681
−4109
−4149
−3619
−3615
−4044
−3885
−4032
−3816
−4355
−4248
−4133
6191
−1329
323



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


304(T)
−353
−974
−2215
−2202
−2896
−1238
−2053
−2560
−2141
−2867
−2052
−1547
−1951
−1940
−2229
1380
3291
−1759
−3143
−2840
324



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


305(C
−845
3595
−3279
−3420
−3342
3140
−2815
−3103
−3232
−3426
−2671
−2300
−2379
−2934
−3057
−1107
−1278
−2270
−3401
−3393
325



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


306(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
326



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


307(K
−2620
−2961
−2461
−2046
−3743
−2791
−1570
−3603
3784
−3387
−2839
−2048
−3039
−1260
−465
−2604
−2536
−3331
−3001
−2988
327



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


308(M
−402
−813
−1034
898
582
−1639
−381
−420
−330
−698
1526
−611
−1715
−218
−668
337
1310
−272
−1195
1104
328



−149
−500
234
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−176
−3166
−8150
−310
−2369
−701
−1378
*
*


309(W
−1600
−1315
−3759
−3181
−295
−3243
−1874
1788
−2803
1756
538
−2802
−3074
−2285
−2600
−2379
−1521
−139
4106
−936
330



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


310(D
−1718
−3250
3403
−27
−3066
−1736
2667
−3486
−1211
−3381
−2732
−400
−2240
−752
−1917
−1439
−1784
−3023
−3204
−2299
331



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


311(G
−1708
−2667
1499
−644
−3784
3218
−1636
−3799
−1910
−3782
−3127
−941
−2466
−1384
−2554
−1618
−1929
−3151
−3633
−3136
332



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


312(K
−708
−2195
956
221
−2514
−1474
−242
−2264
2240
−2194
−1308
1084
−1658
1697
−314
−557
−656
−1821
−2352
−1662
333



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


313(P)
−762
−1504
−940
−454
1424
−1749
−441
−1317
1946
−1439
−747
−629
1988
−176
−300
−813
−723
−1105
−1603
−967
334



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


314(I)
−1232
−1121
−3291
−2953
−1431
−2753
−2412
3122
−2652
−599
−404
−2590
−2982
−2506
−2674
−2020
1485
773
−2421
−2034
335



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


315(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
336



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


316(G)
−1046
−1820
−755
−1003
−3341
2933
−1683
−3325
−1756
−3410
−2651
2259
−2235
−1456
−2142
−1125
−1359
−2537
−3369
−2897
337



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


317(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
338



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


318(W
−3425
−2618
−4155
−4330
3859
−3949
−484
−2303
−3894
−1728
−1759
−2779
−3865
−2847
−3389
−3247
−3323
−2490
3919
1426
339



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


319(R
−1940
−2623
−2163
−1317
−2718
−2498
2633
−2821
557
−2576
−1920
−1294
−2549
−305
3406
−1859
−1734
−2575
−2279
−1885
340



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


320(A
2865
−932
−2456
−2481
−3066
−1198
−2239
−2763
−2443
−3058
−2203
−1637
−1941
−2156
−2474
1737
−732
−1840
−3307
−3058
341



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


321(H
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
342



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


322(Q
−2562
−2904
−1886
−1971
−3251
−2661
−2079
−3690
−1565
−3469
−3081
−2107
−3091
4371
−1665
−2585
−2674
−3411
−3077
−2821
343



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


323(I)
−1757
−1300
−4333
−3971
−1764
−4057
−3754
2871
−3843
−611
−539
−3733
−3880
−3699
−3919
−3375
−1749
2625
−3273
−2829
344



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


324(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
345



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


325(I)
−1899
−1466
−4358
−3871
−1002
−3993
−3149
2479
−3652
1948
170
−3634
−3690
−3153
−3516
−3233
−1847
1460
−2466
−2295
346



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


326(P)
1615
−1136
−2222
−2310
−3082
−1373
−2227
−2731
−2358
−3036
−2272
−1689
3445
−2139
−2424
−761
−938
−1937
−3279
−3042
347



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


327(V
776
−978
−1248
−821
−1609
−1418
−840
−1138
−733
−1453
−710
1478
−1781
−614
−1074
1249
−508
1777
−1905
−1460
348



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


328(D
1973
−1793
2321
−222
−2801
−1401
−864
−2543
−758
−2614
−1785
−457
−1858
−510
−1294
684
−896
−1978
−2851
−2198
349



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


329(Q
−1008
−2231
−986
−310
−2701
−1861
−258
−2315
1642
−2173
−1344
−511
−1911
2494
1827
403
−883
−1946
−2224
−1767
350



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


330(D
−1037
−2659
2253
1117
−2928
−1504
−509
−2731
1324
−2651
−1807
1958
−1830
−106
−940
−826
−1023
−2261
−2827
−2032
351



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


331(H
−935
−2308
2421
146
−2574
−1508
2726
−2388
−331
−2395
−1577
−187
−1822
−135
−890
−787
1407
−1976
−2588
−1852
352



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


332(M
−650
−1996
1349
1466
−2242
−1448
−264
−1958
34
−1982
2809
−101
597
150
−493
−533
−608
−1580
−2227
−1552
353



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


333(E)
1263
−2196
−74
2930
−2933
−1571
−907
−2588
−778
−2712
−1962
−469
−2014
−562
−1290
−991
−1179
−2137
−2954
−2290
354



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


334(H
−1082
−2079
−440
1291
−845
−1821
3880
−1961
−281
−1944
−1264
−509
−2017
−267
−666
−989
−1031
−1699
−1298
1603
355



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


335(F)
1005
−431
−2207
−1610
1664
−1966
−799
1077
332
809
427
−1386
−2016
−1061
−1337
−1023
−432
229
−879
−519
356



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


336(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
357



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


337(D
−996
−2602
2234
1640
−2872
−1493
−484
−2671
−308
−2598
−1748
−104
783
1779
−913
−792
−980
−2205
−2778
−1990
358



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


338(A
1659
−679
−1813
−1259
−745
−1962
−855
1055
−1039
−483
101
−1222
−2054
1021
−1227
−1014
−543
1478
−1248
−857
359



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


339(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
360



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


340(V
−891
−1677
−1034
−464
−1912
−1849
−400
−1350
1652
−1575
−859
−635
−1938
1706
124
−916
−803
2029
−1916
−1472
361



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


341(D
−869
−2445
2202
1077
−2729
120
−389
−2516
1310
−2445
−1574
1245
−1739
29
−730
−684
−841
−2053
−2620
−1858
362



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


342(W
−4114
−3274
−4214
−4453
−1722
−3496
−2681
−4109
−4149
−3619
−3615
−4044
−3885
−4032
−3816
−4355
−4248
−4133
6191
−1329
363



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


343(L)
−2642
−2168
−4788
−4235
−465
−4513
−3241
96
−3878
2891
2122
−4166
−3888
−3023
−3544
−3807
−2521
−607
−2140
−2183
364



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


344(E)
−588
−2030
−204
1763
−2360
−1459
−147
−2094
1522
−2032
−1135
−95
−1592
1372
−129
847
−526
−1661
−2190
−1529
365



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


345(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
366



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


346(Y
−3482
−2868
−3701
−3919
238
−3552
−1112
−3000
−3638
−2516
−2526
−3027
−3772
−3101
−3341
−3418
−3527
−3071
−441
4711
367



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


347(K
−1779
−2672
−1588
−963
−3316
−2329
−561
−2833
3294
−2578
−1879
−1030
−2399
1424
589
−1653
−1579
−2550
−2489
−2237
368



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


348(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
369



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


349(E)
−1283
−2980
2293
2465
−3222
−1551
−680
−3054
846
−2961
−2167
−151
−1956
−302
−1278
−1029
−1292
−2574
−3140
−2287
370



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


350(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
371



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


351(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
372



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


352(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
373



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


353(D
−1170
−2662
2768
152
−3076
−1523
−716
−2882
−677
−2851
−2058
1883
−1940
−347
−1326
−973
1251
−2402
−3056
−2245
374



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


354(E)
1031
−2152
−91
2416
−2507
−1517
−346
−2220
982
−2201
−1350
−188
−1736
71
−388
−657
−752
−1810
−2390
−1731
375



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


355(N
−1247
−2802
2354
164
−3217
−1531
−760
−3060
−767
−2997
−2213
2573
−1971
−395
−1447
1055
−1298
−2553
−3188
−2339
376



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


356(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
377



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


357(H
969
−1896
1173
279
−2199
−1387
1573
−1938
276
−1912
−1011
−34
−1518
1339
−236
939
−421
−1513
−2105
−1426
378



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


358(V
−1097
−861
−3273
−2702
−715
−2790
−1749
1319
−2390
1604
246
−2365
510
−2047
−2299
−1909
−1047
2041
−1564
−1253
379



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


359(K
−627
−934
−1396
−826
−1049
−1906
−680
1291
2041
−745
−142
−930
−1983
−512
−793
−921
762
912
−1475
−1045
380



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


360(D
417
−2242
2268
951
−2534
−1439
−289
−2301
2
−2249
−1365
−64
475
1392
−551
−566
−688
−1853
−2431
−1702
381



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


361(D
−756
−2213
2042
1784
−2484
−1455
−327
−2233
−59
−287
−1351
−90
−1688
90
−612
609
−722
−1813
−2423
−1705
382



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


362(I)
−1758
−1302
−4331
−3969
−1754
−4053
−3745
3003
−3839
−600
−530
−3730
−3876
−3691
−3912
−3370
−1751
2469
−3262
−2822
383



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


363(K
−489
−1736
−399
869
−1996
−1468
−108
−1679
1463
680
−849
−140
−1560
1326
−65
−410
492
−1326
−1942
−1336
384



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


364(E)
1607
−1738
−268
2001
−2459
−1405
−567
−2171
−308
−2231
−1384
−339
−1737
−179
−802
1027
−695
−1699
−2473
−1839
385



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


365(I)
−521
−410
−2443
−1845
1682
−2040
−909
1831
−1536
−217
389
581
−2091
−1245
−1495
−1112
673
1143
−913
−551
386



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


366(M
−1028
−781
−3313
−2728
−655
−2725
−1667
2105
−2396
791
2636
−2332
−2694
−2027
−2260
−1840
662
1307
−1459
−1146
387



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


367(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
388



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


368(K
−420
−1711
−319
889
−1985
−1406
−81
−1683
1799
−1711
−835
−80
−1506
351
−148
802
593
66
−1944
−1311
389



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


369(G
−1210
−2695
1728
114
−3097
2436
−713
−2899
1005
−2848
−2055
−236
−1965
−340
−1178
−1009
−1238
−2429
−3026
−2250
390



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


370(D
−1173
−2847
2185
2156
−3091
−1523
1843
−2917
−527
−2832
−2014
1280
−1899
−221
−1180
−937
−1174
−2440
−3014
−2176
391



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


371(K
−733
−1639
−900
−275
−1870
−1727
−272
−1476
1449
1052
−793
−476
−1798
105
1100
1022
−645
−1222
−1849
−1373
392



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


372(R
−2124
−2788
−2710
−1449
−3624
−2637
−576
−2974
1929
−2640
−1977
−1344
−2596
−161
3417
−2010
−1819
−2745
−2479
−2379
393



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


373(M
−2186
−1770
−4484
−3908
−547
−4083
−2924
1892
−3619
1562
3822
−3715
−3642
−2876
−3343
−3290
−2088
−106
−2072
−2052
394



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


374(A
2525
−1036
−2401
−2529
−3239
2375
−2366
−2958
−2629
−3244
−2393
−1719
−2028
−2303
−2639
−657
−852
−1993
−3425
−3237
395



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


375(M
1980
−959
−1279
−892
−1385
−1524
−842
−905
−763
−1211
2928
1065
−1852
−673
−1053
−675
−559
−683
−1743
−1304
396



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


376(N
−1426
−2353
−948
−735
−2920
−1991
−796
−2769
68
−2666
−1942
3441
−2268
−432
1742
−1361
−1415
−2383
−2592
−2147
397



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


377(P)
−566
−1202
−1850
−1997
−3117
−1371
−2101
−2989
−2185
−3198
−2391
−1549
3625
−1982
−2305
1013
−967
−2094
−3288
−2963
398



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−276
−7108
−2582
−894
−1115
−701
−1378
*
*


378(I)
−820
−759
−2281
−1810
−939
−2345
−1389
2404
−1595
−425
−18
1689
−2430
−1447
−1742
−1465
−804
1594
−1641
−1225
399



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


379(T)
1785
−732
−2107
−1994
−2428
−1065
−1767
−1811
−1860
−2282
−1548
−1351
−1750
−1665
−1956
−390
2954
−1225
−2740
−2439
400



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−453
−1893
*
*


380(N
−1558
−3098
310
1592
−3431
−1657
−945
−3340
−959
−3271
−2572
3495
−2146
−611
−1578
−1301
−1621
−2860
−3383
−2547
401



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


381(G
2030
−1051
−2376
−2525
−3268
2820
−2382
−2992
−2652
−3277
−2428
−1726
−2039
−2322
−2659
−672
−869
−2018
−3447
−3263
402



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


382(G
−1587
−1953
−2017
−2080
−898
3122
−1483
−2565
−2178
−2576
−2101
−1908
−2727
−2015
−2307
−1743
−1817
−2251
−1457
2463
403



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−195
−7108
−3069
−894
−1115
−701
−1378
*
*


383(K
−585
−1070
−1140
−510
−1174
−1742
−358
751
1799
−916
−239
−641
−1802
−103
873
−754
−507
1342
−1431
−1005
404



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−18
−6931
−7973
−894
−1115
−505
−1760
*
*


384(I)
−732
−1273
1962
−516
−1501
−1754
−770
1970
−673
−1163
−572
−718
−1976
−522
−1082
−902
1162
−263
−1908
−1404
405



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


385(H
−542
−1652
−558
18
−1880
−1542
2080
339
360
−1605
−778
−241
1147
1217
1461
−492
−468
−1229
−1864
−1303
406



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


386(K
−682
−1971
−580
740
−2315
−1607
−158
−1989
2284
162
−1077
529
−1686
280
734
−578
−595
−1611
−2087
−1523
407



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


387(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
408



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


388(P)
415
−2181
1668
189
−2543
−1458
−400
−2293
−146
−2281
−1425
−138
2240
1274
−694
−643
−769
−1860
−2492
−1780
409



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


389(L)
−1646
−1694
−2904
−2680
−1050
−2701
−2138
−547
−2336
2673
−123
−2479
1513
−2198
−2336
−2107
−1735
−860
−2077
−1777
410



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


390(D
−430
−1571
1536
153
−1777
−1435
−129
307
1131
−1533
−700
889
−1538
275
−255
−380
−371
598
−1838
−1235
411



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


391(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
412



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


392(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
413



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


393(D
−123
−3021
2652
1083
−3262
−1545
−715
−3102
−722
−3016
−2232
2288
−1970
−344
−1422
−1053
−1333
−2618
−3208
−2331
414



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


394(W
−1183
−940
−3255
−2753
1534
−2632
−772
1086
−2365
1132
71
−2086
−2628
−1856
−2132
−1737
−1115
−336
3856
1849
415



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


395(R
−1025
−2540
1775
1142
−2847
−1561
−472
−2610
−136
−2532
−1695
−183
−1846
−64
2638
−837
−993
−2170
−2686
−1970
416



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


396(D
−1284
−2963
2433
216
−3207
−1549
−693
−3050
−653
−2963
−2174
2058
−1961
2341
−1315
−1034
−1299
−2571
−3142
−2286
417



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


397(Y
−3621
−2707
−4176
−4424
2970
−4050
−394
−2539
−4003
−1942
−1987
−2749
−3933
−2855
−3451
−3299
−3499
−2690
349
4083
418



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


398(C
1443
2403
−1117
1144
−997
−1629
−520
−480
−452
234
−131
−706
−1773
−348
−779
−656
−418
−324
−1362
−922
419



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


399(V
−1742
−1294
−4287
−3868
−1507
−3983
−3425
2151
−3706
586
−316
−3621
−3770
−3449
−3709
−3254
−1716
2871
−2925
−2571
420



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


400(D
−730
−2244
2028
1115
−2543
−1450
−283
−2307
1800
−2248
−1364
−74
−1665
147
−498
−571
502
−1859
−2424
−1702
421



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


401(N
−410
−1793
−248
958
−2083
−143
−54
−1808
1270
−1797
−898
1557
−1482
391
−146
−306
586
53
−2000
−1340
422



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


402(Q
−430
−1511
−419
58
−1825
563
−219
−1494
116
−1597
−764
1033
−1570
1217
−353
1025
−404
892
−1898
−1308
423



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


403(K
42
−2025
1238
279
−2338
−1408
−138
−2090
1948
−2034
−1127
1026
−1554
1361
−253
−410
−491
−1645
−2204
−1512
424



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


404(E)
−625
−2031
−279
1765
−2367
−1499
−161
−2087
353
−2026
−1140
−139
1309
1668
922
−500
−559
−1667
−2180
−1544
425



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1300
−7108
−769
−894
−1115
−701
−1378
*
*


405(Y
−1580
−1211
−2445
−2254
2497
−2497
449
−742
−1944
−476
−322
−1430
−2471
−1343
−1796
−1668
−1507
−820
1088
3543
426



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−560
−1636
*
*


406(G
−1371
−2394
1717
−285
−3391
3003
−1248
−3345
−1454
−3349
−2666
−589
−2148
−975
−2092
−1270
−1563
−2745
−3295
−2730
427



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


407(H
1177
−1467
−267
124
−1912
−1286
2549
−1618
132
−1695
−852
1230
−1499
193
−333
681
−372
−1236
−1957
−1349
428



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


408(N
−352
−1085
−738
−561
−1975
1933
−822
−1555
−672
−1841
−1078
2120
−1685
−547
−1041
−473
−532
758
−2198
−1695
429



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−725
−6850
−1371
−894
−1115
−1215
−813
*
*


409(W
−643
−1210
−455
1427
−532
−1453
−184
−970
−26
−1073
−478
−390
−1652
−90
−302
−705
−607
−812
4240
−180
430



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−348
−6155
−2320
−894
−1115
−1881
−457
*
*


410(G
−461
−838
−976
−1119
−2120
3148
−1219
−2101
−1326
−2273
−1700
−986
−1505
−1212
−1430
−653
−781
−1558
−1923
−1928
431



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−560
−1636
*
*


411(Q
−389
−1056
−645
768
−1124
−1508
−222
−612
34
219
−187
−343
−1598
1815
−346
−489
−329
1199
−1410
−918
432



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−453
−1893
*
*


412(F)
523
−749
−1182
−612
1262
−1690
−436
−327
1202
257
75
−713
−1761
−315
572
−681
−361
558
−1146
−721
433



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


413(Q
−838
−2270
27
1546
−2609
−1503
−383
−2361
−43
−2318
−1463
−158
−1752
2803
−531
937
−810
−1929
−2497
−1800
434



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


414(D
2081
−2191
2846
−271
−3192
−1547
−1150
−2913
−1247
−3052
−2319
−548
−2085
−840
−1860
−1066
−1314
−2364
−3266
−2572
435



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


415(M
746
−729
−1725
−1241
−1091
−1556
−930
−594
−1043
−922
3667
−1111
404
−891
−1250
−698
914
−410
−1509
−1123
436



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


416(I)
−556
−796
−1468
−861
−813
−1848
−559
1435
1067
271
42
−912
−1905
−460
1342
−865
−488
747
−1213
−814
437



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


417(E)
738
−1306
−528
1243
−1448
−1471
−165
−1079
130
−90
−452
−231
−1559
192
330
328
268
267
−1616
−1069
438



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


418(W
−1686
−1388
−3865
−3280
−313
−3346
−1998
1605
−2910
2183
589
−2918
−3142
−2351
−2689
−2486
−1602
−216
3506
−1080
439



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


419(G
−497
−1132
−1973
−2195
−3328
3237
−2289
−3194
−2532
−3410
−2549
−1611
−2057
−2201
−2603
897
−926
−2151
−3487
−3237
440



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


420(K
632
−1777
1362
250
−2087
−1381
−86
−1807
1431
−1808
−914
−51
−1497
355
−193
817
−369
−425
−2019
−1362
441



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


421(Y
−3621
−2707
−4176
−4424
2953
−4049
−394
−2539
−4002
−1942
−1987
−2749
−3933
−2854
−3451
−3299
−3499
−2690
349
4093
442



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


422(C
−642
1771
−2908
−2287
1063
−2214
−1093
1477
−1913
1497
494
−1833
−2239
−1538
−1745
−1304
765
369
−942
−612
443



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


423(R
−1005
−2443
1533
92
−2784
−1584
−463
−2543
−57
−2471
−1639
1177
−1853
−56
2770
−836
−972
−2113
−2612
−1931
444



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


424(D
−1148
−2724
2898
174
−3006
−1564
−566
−2796
951
−2711
−1895
−178
−1903
1815
−851
−933
−1132
−2344
−2872
−2108
445



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


425(V
−1646
−1230
−4148
−3696
−1391
−3782
−3103
2535
−3497
−323
1927
−3416
−3609
−3214
−3466
−3018
−1616
2552
−2681
−2340
446



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


426(I)
401
−900
−3283
−2756
1640
−2752
−1735
2834
−2451
−121
132
−2375
−2775
−2110
−2360
−1897
−1085
615
−1519
−1079
447



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


427(K
−834
−1941
−615
845
−2257
−1704
−312
−1778
2586
−1890
−1101
−402
−1823
95
65
−764
−756
788
−2124
−1599
448



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


428(N
−857
−1859
−733
−254
−2082
−1745
−340
−1726
1897
679
−1022
2134
−1861
45
77
−813
−778
−1455
−2024
−1510
449



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


429(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
450



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


430(P)
−1140
−1553
−2272
−2156
−1935
−1979
−1912
−1526
−1824
−1503
1339
−1897
3724
−1871
−1917
−1397
−1391
−1428
−2485
−2072
451



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


431(H
−464
−1881
1234
255
−2190
−1407
2368
−1921
1008
−1884
−981
−50
−1513
375
482
−349
1263
−1498
−2063
−1403
452



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


432(N
−761
−2121
1175
150
−2581
−1441
−443
−2337
−205
−2327
−1467
2649
−1731
−36
−757
420
1163
−1879
−2535
−1825
453



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


433(F)
−1857
−1592
−3755
−3460
3638
−3255
−1454
−16
−3144
−214
−206
−2800
−3287
−2604
−2951
−2505
−1863
1480
−853
99
454



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


434(R
−2957
−3022
−3318
−2735
−3796
−2998
−1968
−3912
−846
−3631
−3157
−2611
−3280
−1724
4056
−3026
−2913
−3650
−3096
−3185
455



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


435(L)
−886
−722
−2913
−2339
−608
1022
−1423
1356
−2029
1682
294
−2025
−2497
−1718
−1972
−1579
−842
1130
−1337
−1008
456



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


436(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
457



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


437(G
−468
−1108
−1987
−2183
−3307
3093
−2261
−3159
−2489
−3374
−2507
−1593
−2035
−2163
−2570
1410
−895
−2119
−3471
−3217
458



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


438(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
459



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


439(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
460



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


440(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
461



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


441(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
462



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


442(T)
−439
−840
−1804
−1403
−1361
−1523
−1133
−816
−1185
681
−536
−1214
−1931
−1069
−1378
642
2718
−603
−1788
−1389
463



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


443(A
1657
−1594
−404
1150
−1818
−1465
−154
−1471
969
−1566
1309
−173
−1572
248
−202
−422
−412
−1160
−1861
−1272
464



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


444(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
465



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


445(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
466



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


446(R
−1905
−2715
−2334
−1200
−3487
−2506
−485
−2858
1904
−2546
−1846
−1167
−2464
1439
3148
−1780
−1627
−2602
−2423
−2263
467



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


447(L)
−2639
−2158
−4642
−4194
1586
−4340
−2490
−51
−3890
2827
573
−3860
−3832
−2979
−3524
−3629
−2524
−732
−1565
−1006
468



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


448(Q
−549
−1725
−324
98
−1793
−1481
−198
−1668
163
−1723
−892
839
−1618
2328
−290
843
−499
−1331
−1882
1794
469



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


449(A
2109
−2374
1307
1077
−2738
326
−514
−2505
−344
−2485
−1647
−151
−1804
−118
−934
−766
−932
−2058
−2698
−1950
470



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


450(A
1280
−376
−2219
−1624
1011
−1905
−779
1073
−1336
−235
411
−1368
−1975
−1065
−1339
339
580
955
−851
−492
471



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


451(F)
−2619
−2089
−3969
−3846
3011
−3673
−612
−1304
−3453
1530
−788
−2671
−3552
−2584
−3066
−2851
−2522
−1556
92
2879
472



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


452(E)
400
−2342
1163
2306
−2664
−1461
−401
−2438
−168
−2392
−1527
−102
−1735
14
−743
947
−818
−1984
−2583
−1837
473



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


453(H
−424
−834
−1231
−741
−1039
183
2171
−588
−582
−902
−199
−797
−1793
−479
−878
−660
1442
1664
−1404
−972
474



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


454(T)
−825
−1543
−1050
−931
−2411
−1607
−1105
−2069
−661
−2229
−1555
−978
−2065
1333
−881
−930
3234
−1676
−2544
−2072
475



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


455(N
−948
−2217
−192
−59
−2753
871
−516
−2480
1579
−2431
−1604
2747
−1874
−115
−403
−831
−947
−2039
−2574
−1954
476



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


456(R
−2118
−2789
−2710
−1441
−3626
−2635
−569
−2972
2088
−2636
−1971
−1337
−2591
−153
3343
−2003
−1811
−2743
−2476
−2375
477



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−276
−7108
−2582
−894
−1115
−701
−1378
*
*


457(Q
−2077
−2533
−1425
−1467
−2808
−2285
−1598
−3134
−1059
−2958
−2537
−1616
−2694
4251
−1184
−2087
−2168
−2871
−2703
−2369
478



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


458(W
−3599
−2878
−3805
−3989
−1220
−3156
−2211
−3491
−3618
−3057
−3027
−3568
−3532
−3525
−3338
−3829
−3720
−3529
6135
−830
479



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


459(M
−718
−1070
1476
−726
−732
−1903
−713
−152
−681
774
3189
−882
−1991
−541
−1003
−968
−669
−221
−1408
−983
480



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


460(E)
598
−1592
−123
1874
−1828
−1337
−104
−1490
228
−43
−755
967
−1490
296
−254
−355
−375
−1164
−1876
−1258
481



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


461(P)
−556
−1744
1230
129
−2482
1292
−454
−2234
−252
−2255
−1403
−147
1758
−66
−790
781
−633
−1727
−2476
−1801
482



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1043
−6850
−983
−894
−1115
−1215
−813
*
*


462(Y
−1345
−1235
−1689
−1605
1266
−1964
117
−925
−1303
−775
−592
−1236
−2169
−1119
−1309
−1450
−1367
−939
590
3938
483



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−560
−1636
*
*


463(I)
−1578
−1151
−4046
−3597
−1151
−3733
−3046
3001
−3402
771
18
−3355
−3517
−3085
−3372
−2981
−1545
1808
−2540
−2237
484



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−453
−1893
*
*


464(H
−620
−1972
−273
140
−2293
−1493
2910
−2020
1123
−1988
−1114
1414
−1632
244
−127
1037
−563
−1615
−2160
−1523
485



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


465(E)
290
−1861
861
1446
981
−1390
−101
−1871
273
−1861
−968
953
303
339
−239
−355
−411
−1463
−2069
−1398
486



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


466(P)
−546
−1977
862
1025
−2262
−1409
−155
−2002
204
−546
−1086
−48
1748
1636
−313
−420
−495
−1582
−2175
−1489
487



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


467(W
−477
−661
−1479
−920
−458
−1795
−520
−227
−729
−529
143
1513
−1868
−571
−958
304
−420
1257
2766
1177
488



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


468(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
489



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


469(Q
−1336
−2814
130
2632
−3112
−1670
−702
−2921
−403
−2830
−2067
−313
−2037
2655
−853
−1120
−1328
−2494
−2954
−2238
490



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


470(W
−1164
−1540
661
−1080
2483
−2257
1994
−1245
−947
−1304
−742
745
−2310
−770
−1263
−1253
−1097
−1115
2555
1863
491



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


471(M
−459
−840
−1110
504
−907
−1674
−501
−397
−456
−727
3234
−715
−1788
−352
−771
770
−419
680
−1288
−853
492



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


472(H
1702
−1846
−317
119
−2155
−1480
1717
−1860
1008
−1870
−1009
1392
−1616
236
−157
−482
−520
−1482
−2080
−1459
493



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


473(P)
1735
−849
−2213
−1898
−1800
−1446
−1575
−902
−1724
−1568
−933
−1474
2335
−1533
−1863
−706
−666
1535
−2237
−1882
494



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


474(K
373
−1864
703
990
−2179
−1360
−29
−1928
1276
−1877
−955
4
−1460
1260
−124
542
486
−1483
−2049
−1367
495



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


475(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
496



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


476(R
−1165
−2238
−1063
−503
−2794
820
−418
−2401
526
−2281
−1492
−670
−2047
2040
2681
−1075
−1064
−2048
−2328
−1916
497



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


477(V
−823
−660
−2789
−2196
−638
−2422
−1317
1842
−1836
−191
1866
−1913
−2436
−1596
1039
−1512
−768
2028
−1296
−949
498



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


478(V
−1748
−1297
−4301
−3885
−1515
−3999
−3451
2579
−3726
585
−321
−3638
−3782
−3470
−3730
−3273
−1722
2581
−2943
−2589
499



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


479(D
−1067
−2475
2632
1644
−2683
−1544
−584
1013
−469
−2422
−1654
−180
−1882
−209
−1071
−894
−1059
−1933
−2725
−1977
500



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−340
−7108
−2304
−894
−1115
−701
−1378
*
*


480(S)
717
−1527
−107
1500
−2213
−1229
−377
−1911
−112
−1988
−1149
−164
−1554
7
−597
1919
−503
−1461
−2242
−1620
501



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−20
−6788
−7830
−894
−1115
−421
−1984
*
*


481(Q
−790
−1076
−1563
−1026
−923
−2037
−834
−178
−669
−369
1897
−1109
−2120
2919
−906
−1087
−746
1186
−1510
−1104
502



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


482(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
503



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


483(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
504



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


484(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
505



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


485(H
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
506



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


486(Q
−1064
−1426
−1517
−1022
−242
−2170
−535
−884
−583
715
−396
−1085
−2235
−3051
−804
−1225
−994
−864
−835
1693
507



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


487(C
1096
3020
1354
−606
−1109
−1571
−566
−613
−497
−939
1724
−707
−1762
−380
−838
−622
−428
−433
−1460
−1015
508



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


488(E)
−1401
−2897
169
3007
−3185
−1677
−753
−3009
−498
−2916
−2170
−319
−2069
1880
−966
−1173
−1402
−2580
−3040
−2307
509



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


489(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
510



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


490(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
511



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


491(W
−2172
−1773
−3771
−3493
3388
−3396
−717
−939
−3099
−528
2179
−2555
−3307
−2387
−2796
−2550
−2089
−1165
3733
1010
512



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


492(L)
−2620
−2150
−4778
−4218
−461
−4493
−3219
96
−3870
2765
2758
−4143
−3872
−3009
−3534
−3777
−2498
−607
−2129
−2177
513



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


493(E)
−1401
−2897
169
3007
−3185
−1677
−753
−3009
−498
−2916
−2170
−319
−2069
1880
−966
−1173
−1402
−2580
−3040
−2307
514



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


494(G
2172
−1046
−2384
−2525
−3259
2719
−2377
−2982
−2644
−3267
−2417
−1723
−2035
−2315
−2652
−666
−864
−2010
−3440
−3255
515



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


495(Y
−3482
−2868
−3701
−3919
238
−3552
−1112
−3000
−3638
−2516
−2526
−3027
−3772
−3101
−3341
−3418
−3527
−3071
−441
4711
516



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


496(T)
−971
−915
−2809
−2296
−857
−2442
−1626
433
−1998
1151
92
−2043
−2569
−1769
−2033
−1592
2524
1200
−1666
−1332
517



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


497(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
518



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


498(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
519



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


499(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
520



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


500(R
−2957
−3022
−3318
−2735
−3796
−2998
−1968
−3912
−846
−3631
−3157
−2611
−3280
−1724
4056
−3026
−2913
−3650
−3096
−3185
521



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


501(H
−999
−1647
−1206
−1105
−1780
−1755
4476
−2154
−805
−2276
−1630
−1129
−2202
−979
−999
−1106
1428
−1786
−2106
−1395
522



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


502(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
523



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


503(I)
−1567
−1223
−3950
−3399
2055
−3419
−2370
2527
−3104
944
309
−3048
−3245
−2620
−2927
−2582
−1507
1309
−1899
−1632
524



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


504(W
−3425
−2618
−4155
−4330
3859
−3949
−484
−2303
−3894
−1728
−1759
−2779
−3865
−2847
−3389
−3247
−3323
−2490
3919
1426
525



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


505(A
2014
−983
−1788
−1434
−2527
−1231
−1430
−2209
−1330
−2413
−1572
−1164
1491
−1179
−1621
1494
990
−1548
−2719
−2324
526



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


506(S)
−352
−985
−2207
−2221
−3013
−1228
−2099
−2715
−2192
−3001
−2167
−1553
−1952
−1978
−2279
3131
1106
−1840
−3241
−2944
527



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


507(Y
−3482
−2868
−3701
−3919
238
−3552
−1112
−3000
−3638
−2516
−2526
−3027
−3772
−3101
−3341
−3418
−3527
−3071
−441
4711
528



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


508(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
529



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


509(S)
1981
−864
−2260
−1969
−2205
−1291
−1697
−1605
−1808
−2070
−1333
−1448
−1910
−1601
−1958
2200
−636
877
−2550
−2211
530



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


510(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
531



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


511(V
−929
−722
−3094
−2519
−671
72
−1536
1450
−2197
1669
235
−2159
−2584
−1873
−2107
−1688
−882
1742
−1406
−1072
532



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


512(R
−1152
−2148
−1103
−556
−2568
841
2681
−2297
448
−2220
−1446
−714
−2063
−63
2686
−1086
−1067
−1962
−2250
−1802
533



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


513(V
−1261
−1032
−2990
401
−1324
−3032
−2128
1904
−2354
−591
−293
−2430
−3023
−2199
−2472
−2185
−1232
2780
−2221
−1813
534



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


514(V
−1755
−1298
−4332
−3970
−1769
−4057
−3755
2730
−3843
−618
−544
−3732
−3880
−3701
−3920
−3374
−1748
2759
−3277
−2831
535



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


515(D
−1718
−3250
3403
−27
−3066
−1736
2667
−3486
−1211
−3381
−2732
−400
−2240
−752
−1917
−1439
−1784
−3023
−3204
−2299
536



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


516(S)
−635
−1657
1163
−252
−2614
−1382
−780
−2338
−601
−2421
−1582
−466
−1801
−415
−1101
2397
1307
−1804
−2665
−2044
537



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


517(M
−2406
−2296
−3638
−3594
−1525
−3105
−2824
−1047
−3121
−596
5043
−3293
−3425
−3046
−2996
−2911
−2552
−1398
−2513
−2207
538



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


518(M
−2437
−1975
−4738
−4148
−487
−4369
−3139
1386
−3872
2524
2731
−4019
−3789
−2984
−3525
−3603
−2318
−374
−2113
−2174
539



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


519(T)
−504
−959
−1413
−1049
−1473
−1580
−994
−629
−943
−1256
−601
1702
−1931
−837
−1224
−750
1993
1780
−1865
−1432
540



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


520(Q
−2562
−2904
−1886
−1971
−3251
−2661
−2079
−3690
−1565
−3469
−3081
−2107
−3091
4371
−1665
−2585
−2674
−3411
−3077
−2821
541



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


521(H
−2992
−2589
−3064
−3043
863
−3512
4371
−2521
−2584
−2051
−1961
−2327
−3545
−2255
−2492
−2815
−2919
−2563
158
3265
542



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


522(F)
1777
−758
−2605
−2069
2426
−2201
−1138
17
−1776
−51
1943
−1762
−2314
−1474
−1762
−1320
−786
−23
−1063
−575
543



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


523(K
−2620
−2961
−2461
−2046
−3743
−2791
−1570
−3603
3784
−3387
−2839
−2048
−3039
−1260
−465
−2604
−2536
−3331
−3001
−2988
544



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


524(W
−3358
−2599
−4116
−4259
2442
−3892
−536
−2247
−3821
−1683
−1712
−2792
−3835
−2843
−3352
−3227
−3271
−2441
5485
1363
545



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


525(L)
841
−1175
−3469
−2926
−668
−3037
−2058
1328
−2615
2287
379
−2642
−2978
−2226
−2526
−2188
−1355
301
−1760
−1526
546



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


526(R
−837
−2176
−602
1392
−2587
−1688
−216
−2257
1534
−2141
−1281
1107
−1779
227
−2109
−709
−741
−1854
−2229
−1683
547



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


527(M
888
−1677
−357
850
−1922
−1428
1639
−1611
1435
−1654
1807
−103
−1521
338
−129
−358
−369
−1259
−1902
−1285
548



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


528(A
2576
−902
−2349
−2158
−2756
−1202
−1937
−2410
−2031
−2688
−1852
−1502
−1892
−1801
−2162
1022
1811
−1644
−3004
−2709
549



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


529(K
−583
−1746
−490
34
−1989
−1543
1732
−1666
1763
−1714
−880
1553
−1641
238
−31
−516
−513
875
−1945
−1367
550



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1300
−7108
−769
−894
−1115
−701
−1378
*
*


530(R
−915
−1387
−1141
−641
−1786
−1421
−246
−1638
712
−1598
−1052
−657
−1663
43
3215
−962
−890
−1418
−1542
−1280
551



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−238
−2716
*
*


531(E)
−1710
−3547
2209
3007
−3749
−1634
−990
−3679
−1227
−3559
−2898
−238
−2166
−662
−2065
−1376
−1779
−3163
−3720
−2744
552



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


532(I)
−599
−507
−2385
−1791
−457
−2112
−969
1604
−1499
1009
366
−1553
−2152
765
−1506
−1178
1103
1145
−1019
−667
553



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


533(P)
787
−1572
724
108
−1851
−1418
−201
−1534
126
−1626
−789
−169
1640
198
−358
575
−413
−1199
−1916
922
554



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


534(W
−4114
−3274
−4214
−4453
−1722
−3496
−2681
−4109
−4149
−3619
−3615
−4044
−3885
−4032
−3816
−4355
−4248
−4133
6191
−1329
555



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


535(R
−1940
−2623
−2163
−1317
−2718
−2498
2633
−2821
557
−2576
−1920
−1294
−2549
−305
3406
−1859
−1734
−2575
−2279
−1885
556



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


536(H
−1345
−2440
−1207
−576
−2915
−2072
3063
−2527
2588
−2343
−1569
−727
−2129
1381
532
−1222
−1187
−2200
−2324
−1939
557



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


537(D
−695
−2182
1890
241
−2491
−1456
−249
−2246
1496
−2187
−1300
−85
1264
1306
−385
−545
−648
−1804
−2357
−1656
558



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


538(Y
−1138
−876
−3345
−2810
−645
−2852
−1674
1928
−2488
−317
10
−2411
−2835
−2166
−2393
−1984
−1094
2117
−1423
2681
559



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


539(P)
1031
−974
−2031
−1918
−2925
−1207
−1878
−2641
−1913
−2865
−2004
−1407
2879
−1695
−2096
1838
−694
−1790
−3123
−2802
560



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


540(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
561



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


541(S)
1737
−944
−2392
−2415
−3092
−1199
−2214
−2801
−2395
−3086
−2226
−1614
−1940
−2116
−2445
2898
−735
−1863
−3322
−3065
562



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


542(M
−1968
−1576
−4331
−3761
−611
−3859
−2765
1741
−3480
2227
2250
−3502
−3515
−2812
−3229
−3049
−1885
1013
−2038
−1963
563



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


543(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
564



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


544(I)
−1257
−1007
−3424
−2868
1397
−2811
−1254
2188
−2500
1333
382
−2341
−2749
−1996
−2283
−1922
−1186
−115
−815
2061
565



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


545(I)
−1831
−1391
−4327
−3871
−1200
−3991
−3265
2941
−3675
1302
−22
−3631
−3727
−3275
−3598
−3245
−1791
1578
−2650
−2410
566



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


546(E)
999
−1712
787
1203
−1974
−1412
−152
−1661
194
−1721
−863
−101
−1546
266
−305
466
−419
822
−1983
−1349
567



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


547(T)
590
−1479
974
−206
−2293
−1346
−555
−1995
−272
−2077
−1218
−407
−1671
−167
−745
1655
1771
−1514
−2333
−1741
568



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


548(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
569



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


549(T)
−866
−1511
−1211
−1054
−1813
−1680
2857
−1969
−793
−2130
−1455
−1074
−2117
−914
−1013
−989
3041
−1609
−2119
−1469
570



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


550(W
−587
−608
−2405
−1898
−479
−1928
−993
−42
−1552
−528
62
−1554
−2129
−1332
−1550
365
−609
2511
3028
−502
571



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


551(W
947
−1346
−3135
−2759
3172
−2759
−620
−882
−2412
−912
−526
−2088
−2831
−1938
−2273
−1901
−1498
−870
3648
929
572



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


552(Q
−1772
−2604
−1870
−1079
−3217
−2374
−569
−2777
660
−2529
−1835
−1110
−2420
3075
2723
−1671
−1571
−2504
−2437
−2192
573



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


553(Q
−2562
−2904
−1886
−1971
−3251
−2661
−2079
−3690
−1565
−3469
−3081
−2107
−3091
4371
−1665
−2585
−2674
−3411
−3077
−2821
574



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


554(D
−1791
−3663
3414
1659
−3853
−1647
−1043
−3807
−1343
−3679
−3054
−251
−2201
−725
−2227
−1438
−1872
−3282
−3836
−2831
575



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


555(H
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
576



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


556(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
577



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


557(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
578



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


558(Y
−3620
−2706
−4179
−4428
3303
−4051
−393
−2536
−4005
−1939
−1984
−2749
−3933
−2855
−3452
−3299
−3498
−2688
350
3843
579



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


559(S)
−344
−978
−2187
−2155
−2953
−1227
−2035
−2643
−2108
−2926
−2093
−1523
−1940
−1902
−2216
2907
1859
−1800
−3181
−2875
580



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


560(H
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
581



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


561(Q
−2562
−2904
−1886
−1971
−3251
−2661
−2079
−3690
−1565
−3469
−3081
−2107
−3091
4371
−1665
−2585
−2674
−3411
−3077
−2821
582



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


562(D
−1724
−3448
3411
103
−3726
−1642
−1058
−3734
−1342
−3628
−2991
1884
−2196
−745
−2191
−1408
−1822
−3197
−3743
−2768
583



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


563(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
584



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


564(G
−468
−1108
−1987
−2183
−3307
3093
−2261
−3159
−2489
−3374
−2507
−1593
−2035
−2163
−2570
1410
−895
−2119
−3471
−3217
585



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


565(V
371
−671
−3209
−2618
1562
−2580
−1515
1368
−2275
648
269
−2195
−2574
−1917
−2132
−1689
−852
2127
−1337
−1001
586



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


566(T)
−1238
−1032
−3270
−2717
−705
−2883
−1871
1700
−2401
1666
301
−2442
−2848
−2072
−2345
−2015
1957
452
−1675
−1395
587



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


567(T)
−307
−965
−2051
−1918
−2899
1272
−1866
−2617
−1903
−2838
−1975
−1405
−1889
−1683
−2089
1577
2739
−1773
−3100
−2780
588



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


568(H
837
−578
−1703
−1139
−579
−1822
2864
−45
−902
−418
1836
−1086
−1915
−740
−1071
−867
−423
1342
−1041
−648
589



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


569(L)
−2092
−1719
−4323
−3846
−712
−3925
−2989
451
−3531
2641
428
−3624
−3640
−2952
−3339
−3191
−2044
1542
−2221
−2096
590



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


570(L)
1056
−648
−2733
−2160
1291
−2277
−874
5
−1827
1649
341
−1760
−2303
−1464
−1729
−1359
−733
−2
−647
1605
591



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


571(E)
−994
−2497
67
2352
−2818
−1564
−437
−2572
1391
−2487
−1645
1818
−1831
−24
−503
−812
−956
−2133
−2633
−1933
592



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


572(K
−1592
−2196
−1883
−1179
−2203
−2360
−733
−1718
3129
666
−1212
−1218
−2435
−383
258
−1621
−1460
−1669
−2199
−1837
593



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−971
−7108
−1051
−894
−1115
−701
−1378
*
*


573(C
−39
2488
−473
1144
−1043
−1085
−142
−526
67
−851
−125
−185
−1325
126
−296
−170
1058
−295
−1316
−837
594



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−360
−6167
−2274
−894
−1115
−1874
−459
*
*


574(F)
−1315
−1110
−2234
−2129
3522
−2099
−247
−203
−1900
58
60
−1632
−2283
−1510
−1804
−1624
−1357
−396
303
1334
595



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


575(H
−354
−1360
326
436
−1031
−1026
2689
−1410
436
−1450
−724
1667
−1303
383
89
−286
−351
−1096
−1280
−501
596



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−238
−2716
*
*


576(K
−1086
−2304
−1032
−362
−2797
−1910
−283
−2399
2361
−2235
−1413
1695
−1959
1479
1362
−963
−952
−2031
−2264
−1826
597



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


577(P)
−1766
−2832
2091
−503
−3634
−1897
−1480
−3629
−1677
−3608
−2962
−830
3445
−1206
−2322
−1624
−1926
−3087
−3516
−2951
598



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


578(H
−480
−1902
1434
926
−2176
−1399
2271
−1932
300
−1903
−1004
−39
−1521
347
782
−362
−423
−1511
−2083
1389
599



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


579(Y
839
−383
−2642
−2032
1206
−2056
−863
223
−1678
569
461
−1617
−2100
−1333
−1555
−1135
−472
1465
−747
1987
600



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


580(I)
865
−836
−3163
−2646
−1108
−2692
−1893
2677
−2382
−556
−157
−2324
−2771
−2135
−2380
−1845
504
1519
−1868
−1491
601



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


581(R
−1066
−2251
−943
−347
−2740
308
−313
−2349
1342
−2216
−1401
854
−1954
119
2761
−953
−949
−1986
−2272
−1823
602



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


582(E)
−370
−640
−961
1875
−770
−1558
−279
900
−284
−670
84
−547
−1598
−160
−606
−577
−301
965
−907
−668
603



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


583(Y
−3618
−2707
−4172
−4418
2748
−4047
−395
−2537
−3997
−1940
−1986
−2748
−3932
−2853
−3448
−3297
−3496
−2688
348
4199
604



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


584(F)
−2597
−2122
−4519
−4116
2769
−4202
−2114
−140
−3797
2428
469
−3639
−3772
−2912
−3436
−3471
−2488
−783
−1265
−532
605



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


585(P)
1615
−1136
−2222
−2310
−3082
−1373
−2227
−2731
−2358
−3036
−2272
−1689
3445
−2139
−2424
−761
−938
−1937
−3279
−3042
606



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


586(A
2397
−935
−2082
−1846
−2575
−1236
−1722
−2190
−1737
−2475
−1666
−1377
1554
−1552
−1929
−527
1772
−1532
−2826
−2490
607



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


587(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
608



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


588(A
3098
−930
−2580
−2583
−2894
−1243
−2249
−2309
−2448
−2781
−2022
−1697
−1976
−2194
−2449
−591
1113
−1602
−3196
−2952
609



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


589(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
610



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


590(M
−387
−854
−1643
−1199
−1437
−1461
−1020
−962
−1012
−1269
2610
−1075
−1857
−895
−1259
1806
1865
−709
−1810
−1406
611



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


591(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
612



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


592(L)
−2625
−2145
−4613
−4174
1968
−4304
−2386
−72
−3866
2741
551
−3801
−3814
−2959
−3500
−3585
−2511
−745
−1483
−874
613



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


593(L)
−2193
−1789
−4457
−3877
−523
−4057
−2894
279
−3584
2588
2231
−3689
−3624
−2841
−3310
−3261
−2096
1093
−2048
−2035
614



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


594(A
2970
−925
−2555
−2528
−2849
−1243
−2202
−2276
−2388
−2737
−1975
−1675
−1968
−2140
−2406
−585
1607
−1581
−3151
−2898
615



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


595(V
−1334
−1034
−3629
−3155
−1390
−3214
−2516
2107
−2918
−591
−331
−2852
−3211
−2701
−2928
−2407
1824
2364
−2380
−1985
616



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


596(M
998
−406
−1988
−1399
1077
−46
−692
73
−1142
733
1731
−1227
−1918
−903
−1209
286
−359
167
−855
−487
617



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


597(H
−1095
−2732
1291
2125
−2985
−1512
3032
−2799
−427
−2721
−1890
1256
−1861
−159
−1055
−875
−1088
−2329
−2902
−2089
618



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


598(H
−971
−1917
−1026
−424
−2119
−1866
2995
−1811
2402
−1852
−1093
−602
−1947
34
266
−932
−871
294
−1999
−1507
619



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


599(C
1836
3931
−2905
−2499
−1252
−1823
−1705
1152
−2189
−888
−389
−1893
−2246
−1917
−2150
−1063
−756
157
−1855
−1509
620



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


600(F)
528
−777
−2899
−2354
2441
−2415
−823
−127
−2008
1363
215
−1876
−2433
−1604
−1876
−1506
−889
−142
−490
1736
621



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


601(K
−1239
−2390
−1266
−522
−2934
−2031
−324
−2495
2422
−2300
−1501
979
−2062
1504
1964
−1116
−1081
−2146
−2296
−1915
622



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


602(S)
699
−1151
−903
−440
−1699
−1408
−579
−1323
−350
−1526
1574
974
−1694
−269
−750
2107
−464
−1002
−1909
−1408
623



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


603(Q
−873
−2060
−736
−198
−2478
−1732
−282
−2118
1944
−2060
−1235
−426
−1836
1968
230
−780
1853
−1758
−2189
−1681
624



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


604(N
−1080
−2639
214
1668
−2924
−1545
−527
−2711
−282
−2635
−1807
2572
−1867
2104
−814
−876
−1062
−2261
−2801
−2043
625



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


605(K
−457
1622
−593
−29
−1532
−1520
−184
−1170
1745
−1320
−528
−275
−1605
1259
−206
−467
609
−916
−1665
1434
626



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


606(I)
−1995
−1561
−4353
−3926
−1045
−4065
−3261
3346
−3652
924
94
−3716
−3783
−3240
−3554
−3362
−1957
705
−2551
−2295
627



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


607(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
628



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


608(Y
1025
−487
−2483
−1892
−336
−2087
−935
224
−1576
1270
403
−1587
−2140
−1281
−1535
−1165
−537
999
−908
2074
629



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


609(M
−797
−587
−3073
−2468
1091
−2423
−1329
2169
−2112
−34
2364
−2036
−2429
−1745
−1959
−1522
645
1209
−1165
−836
630



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


610(V
−1573
−1179
−4043
−3576
867
−3627
−2822
2175
−3353
−312
−198
−3256
−3486
−3047
−3296
−2841
−1540
2804
−2430
−2026
631



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


611(A
2440
−912
−2339
−2201
−2840
1287
−1997
−2505
−2116
−2780
−1935
−1522
−1901
−1868
−2234
−517
1851
−1699
−3081
−2799
632



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


612(S)
−437
−1083
−2006
−2144
−3262
1583
−2197
−3097
−2380
−3307
−2434
−1562
−2008
−2077
−2485
2965
−857
−2074
−3426
−3160
633



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


613(K
−2620
−2961
−2461
−2046
−3743
−2791
−1570
−3603
3784
−3387
−2839
−2048
−3039
−1260
−465
−2604
−2536
−3331
−3001
−2988
634



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


614(H
−1501
−2478
−747
−589
−1969
−2033
3845
−2617
−5
−2483
−1816
−796
−2272
2881
−187
−1387
−1442
−2314
−2088
−1345
635



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


615(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
636



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


616(R
1242
−1347
−1254
−731
−2201
−1510
−719
−1792
−153
−1944
−1159
−782
−1844
−375
2580
−656
1294
−1393
−2232
−1788
637



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


617(P)
1448
−1164
−798
−305
−1387
−1533
−406
−980
−151
81
−453
−488
1572
1113
−529
−556
−448
−759
−1638
−1144
638



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


618(Q
−557
−1175
−1028
−563
−1488
−1618
−611
−820
−317
−1222
−557
−696
−1835
2724
−638
−698
1378
1031
−1782
−1308
639



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


619(W
−3603
−2685
−4198
−4439
2092
−4053
−373
−2561
−4004
−1981
−2004
−2736
−3927
−2842
−3443
−3284
−3472
−2688
5224
2885
640



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


620(F)
−1507
−1226
−3587
−3073
2681
−3022
−1217
950
−2707
1659
313
−2490
−2927
−2137
−2464
−2143
−1430
−380
−687
1671
641



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


621(S)
−344
−978
−2188
−2158
−2955
−1227
−2038
−2646
−2111
−2929
−2096
−1524
−1940
−1904
−2218
2919
1829
−1801
−3183
−2878
642



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


622(I)
−1092
−874
−3179
−2611
−721
−2765
−1721
1891
−2310
1192
241
−2311
1836
−1986
−2249
−1880
−1042
1298
−1570
−1257
643



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


623(D
−891
−2456
2161
1856
−2735
−1474
−410
−2519
−186
−2456
−1593
−91
−1753
1291
−767
−707
782
−2062
−2639
−1876
644



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


624(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
645



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


625(A
3438
−1472
−2846
−3040
−3287
−1726
−2735
−2840
−3028
−3257
−2662
−2236
−2447
−2798
−2944
−1216
−1387
−2183
−3405
−3320
646



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


626(K
−719
−2060
−577
1623
−2444
−1622
−164
−2125
1699
−2035
−1164
−266
−1703
282
1624
−601
634
−1722
−2150
−1583
647



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


627(Q
1243
−1432
−728
−405
−2358
−1377
−690
−2055
−351
−2150
−1310
−555
−1744
2880
−751
1081
−618
−1561
−2401
−1849
648



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


628(H
−780
−1536
−555
1361
−1393
−1709
2249
−1189
−144
1529
−640
−494
−1865
−169
−489
−816
−724
−1022
−1690
−1099
649



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


629(C
456
2634
−3043
−2479
−719
−2386
−1463
624
−2148
1022
146
−2055
−2467
−1831
−2047
−1515
−777
2135
−1370
−1020
650



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


630(D
59
−1947
1795
1154
−2254
−1392
−111
−1999
273
−1959
−1053
−30
−1526
335
1177
−370
709
−1564
−2142
−1456
651



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


631(Q
1161
−1938
−287
164
−2274
−1471
−154
−1990
1104
−1956
−1072
1604
−1598
1906
−101
−461
−513
−1579
−2132
−1496
652



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


632(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
653



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


633(L)
2002
−1175
−2785
−2475
−1055
−2185
−1898
−221
−2172
2054
−104
−2097
−2542
−1964
−2186
−1458
−1162
−325
−1982
−1669
654



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


634(K
943
−1709
−403
133
−2096
781
−142
−1801
994
−1811
−930
−144
−1543
292
938
844
−405
−1399
−2023
−1402
655



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


635(I)
525
−452
−2111
23
−445
−1963
−805
1776
−1266
−283
345
−1346
−2019
−1026
−1323
−1017
−436
1518
−942
1179
656



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


636(W
−1626
−1321
−3570
−3141
2053
−3057
−937
2580
−2760
−226
−54
−2426
−2999
−2185
−2514
−2189
−1555
−448
3586
581
657



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


637(D
−1498
−3029
3115
17
−3354
−1691
−852
−3199
1912
−3109
−2371
−341
−2120
−495
−1230
−1252
−1519
−2742
−3208
−2450
658



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


638(W
−3358
−2599
−4116
−4259
2442
−3892
−536
−2247
−3821
−1683
−1712
−2792
−3835
−2843
−3352
−3227
−3271
−2441
5485
1363
659



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


639(C
3004
3179
−3175
−3184
−2750
−1300
−2459
−2082
−2872
−2641
−1933
−1922
−2041
−2538
−2722
−657
−796
−1473
−3106
−2895
660



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


640(S)
−897
−1462
−2333
−2543
−3185
−1640
−2474
−3294
−2686
−3497
−2780
−1973
−2360
−2483
−2703
3465
−1316
−2413
−3310
−3025
661



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


641(T)
−638
−1323
−1200
−1263
−2717
−1420
−1550
−2421
−1436
−2697
−1948
1009
−2035
−1340
−1682
−796
3384
−1817
−2917
−2452
662



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


642(H
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
663



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


643(D
1256
−1810
2828
−193
−2711
−1421
−825
−2377
−710
−2507
−1697
−438
−1858
−471
−1243
−735
787
−1875
−2779
−2131
664



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


644(K
−1038
−2259
−689
−253
−2738
777
−360
−2382
2468
−2269
−1451
−481
−1931
2027
226
−921
−949
−2002
−2343
−1850
665



−149
−500
233
43
−381
398
106
−626
210
−466
−721
276
395
45
96
360
118
−369
−294
−249



−535
−1708
−8150
−140
−3430
−701
−1378
*
*


645(G
−1044
−1813
−773
−1023
−3345
2987
−1699
−3331
−1775
−3418
−2659
2131
−2239
−1475
−2156
−1127
−1361
−2539
−3373
−2907
667



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


646(E)
−488
−1836
1360
1439
−2104
−1403
−131
−1820
229
87
−955
−60
−1537
301
−280
469
631
−1431
−2061
−1399
668



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


647(E)
−1127
−2366
−800
2480
−2838
−1878
−336
−2455
1396
−2301
−1488
−522
−1975
95
1670
−993
−1007
−2085
−2343
−1881
669



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


648(P)
955
−915
−2251
−2048
−2204
−1361
−1790
−1375
−1894
−1974
−1329
−1523
3265
−1711
−2016
−660
−714
631
−2606
−2267
670



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


649(D
−1535
−3339
3248
856
−3535
−1586
−853
−3417
−967
−3307
−2587
−180
−2074
1731
−1720
−1229
−1578
−2919
−3496
−2555
671



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


650(V
−1754
−1297
−4329
−3968
−1770
−4053
−3752
2602
−3840
−621
−545
−3728
−3878
−3699
−3917
−3370
−1746
2861
−3276
−2829
672



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


651(V
−1736
−1298
−4275
−3915
−1733
−3969
−3654
1867
−3766
−598
−525
−3663
−3828
−3619
−3833
−3283
−1733
3222
−3208
−2763
673



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


652(M
−2158
−1763
−4466
−3857
2098
−3961
−2711
1339
−3551
2194
2215
−3609
−3535
−2747
−3220
−3143
−2048
−438
−1879
−1836
674



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


653(A
2500
−821
−2504
−2171
−1756
−1515
−1719
−589
−1953
−1465
−872
−1633
−2062
−1734
−2039
−780
1073
1394
−2251
−1909
675



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


654(A
2412
−931
−2308
−2228
−3059
1309
−2079
−2797
−2207
−3025
−2139
−1529
−1901
−1934
−2326
1722
−691
−1849
−3263
−2994
676



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


655(A
2464
−791
−2155
−1787
−1514
−1500
−1408
1386
−1595
−1309
−672
−1424
−1982
−1405
−1732
688
−624
−473
−1963
−1596
677



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


656(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
678



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


657(T)
−457
−1344
1397
−166
−1749
−1438
−424
−1324
−173
−1546
−757
−384
−1663
−77
−623
628
1799
847
−1920
−1372
679



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


658(E)
−1108
−1855
−497
2835
−2161
−1834
−982
−964
−783
−1744
−1237
−739
−2163
−677
−1197
−1164
−1148
1427
−2502
−1927
680



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


659(P)
−853
−1252
−2460
−2393
−2139
−1784
−2109
−974
−2210
−1748
−1364
−1924
3453
−2108
−2271
−1167
−1152
1448
−2678
−2307
681



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


660(T)
−495
−998
−1197
−789
−1334
−1567
−751
−851
−618
−1153
2261
892
−1849
−564
−910
−695
2581
−648
−1684
−1235
682



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


661(Q
−425
−1001
−831
629
1174
−1595
−316
−637
−152
579
−150
−476
−1677
1648
−532
−563
735
−470
−1354
−875
683



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


662(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
684



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


663(I)
525
−535
−2111
−1551
−638
−1888
−920
1560
−1309
−455
170
−1362
−2031
−1084
−1399
1061
708
1332
−1134
−763
685



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


664(M
−2362
−1913
−4673
−4086
−501
−4285
−3077
1692
−3808
2336
2939
−3933
−3746
−2955
−3477
−3510
−2250
−300
−2098
−2139
686



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


665(A
2734
−1554
−1235
−966
−2607
−1620
−1015
−2163
1487
−2343
−1601
−988
−2045
−691
−583
−903
−969
−1725
−2578
−2168
687



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


666(A
3438
−1472
−2846
−3040
−3287
−1726
−2735
−2840
−3028
−3257
−2662
−2236
−2447
−2798
−2944
−1216
−1387
−2183
−3405
−3320
688



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


667(I)
1114
−756
−2253
−1864
−1305
−1646
−1394
2517
−1656
−1054
−461
−1512
−2066
−1454
−1761
1262
−650
−138
−1806
−1439
689



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


668(D
900
−2457
2570
175
−2815
−1502
−553
−2590
−386
−2564
−1737
−168
−1841
2095
−969
−821
−1001
−2141
−2768
−2010
690



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


669(I)
292
−707
−2167
−1590
−719
−2180
−1027
2540
537
441
146
−1486
−2230
−1118
−1321
−1247
−668
405
−1287
−917
691



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


670(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
692



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


671(H
−1177
−2326
−1134
−457
−2809
−1977
3593
−2401
1445
−2244
−1443
844
−2024
111
1735
−1061
−1033
−2058
−2268
−1857
693



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


672(E)
15
−2170
1563
1959
−2472
−1425
−226
−2237
855
−2177
−1280
−49
−1617
1268
−431
−502
−609
−1785
−2349
−1631
694



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


673(H
−403
−1628
−358
195
−1861
−1414
1618
−1548
320
−279
1074
912
−1503
1197
1139
446
−340
−1203
−1863
−1247
695



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


674(C
−513
2550
−2600
−2019
2246
845
−943
1137
−1680
−244
350
−1605
−2090
−1357
−1587
−1083
−488
249
−881
−474
696



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−340
−7108
−2304
−894
−1115
−701
−1378
*
*


675(P)
−2312
−2374
−2796
−3036
−3591
−2432
−2883
−3881
−3157
−3835
−3473
−2848
4156
−3101
−3072
−2546
−2648
−3391
−3143
−3459
697



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−20
−6788
−7830
−894
−1115
−1306
−747
*
*


676(E)
−985
−2607
1546
2542
−2883
−1323
−441
−2699
−378
−2634
−1828
58
−1713
−60
−1032
869
−997
−2229
−2828
−1997
698



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−20
−6788
−7830
−894
−1115
−421
−1984
*
*


677(L)
−2103
−1693
−4449
−3873
−568
−4003
−2874
1606
−3596
2406
2166
−3648
−3596
−2862
−3318
−3203
−2010
674
−2059
−2029
699



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


678(K
−2059
−2767
−2575
−1375
−3583
−2596
−558
−2947
3236
−2620
−1947
−1295
−2560
−141
2026
−1942
−1766
−2709
−2469
−2350
700



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


679(I)
−1544
−1176
−3965
−3470
1894
−3502
−2556
2750
−3215
−120
−27
−3124
−3366
−2846
−3113
−2694
−1504
1816
−2142
−1735
701



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


680(R
−2118
−2789
−2710
−1441
−3626
−2635
−569
−2972
2088
−2636
−1971
−1337
−2591
−153
3343
−2003
−1811
−2743
−2476
−2375
702



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


681(Y
−1738
−1421
−3486
−3138
2368
−3054
−658
−643
−2769
−827
−509
−2313
−3036
−2175
−2527
−2188
−1672
2239
−69
2812
703



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


682(V
−1754
−1297
−4329
−3968
−1770
−4053
−3752
2614
−3840
−621
−545
−3729
−3878
−3699
−3917
−3370
−1746
2852
−3276
−2830
704



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


683(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
705



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


684(V
−1733
−1299
−4267
−3907
−1727
−3955
−3640
1809
−3755
−594
−523
−3653
−3820
−3608
−3821
−3270
−1732
3240
−3198
−2753
706



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


685(V
484
−646
−2970
−2395
−744
−2499
−1461
1423
−2091
572
130
−2055
−2521
−1798
−2031
−1603
1127
2072
−1395
−1034
707



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


686(D
−1820
−3681
3540
1270
−3878
−1659
−1068
−3841
−1383
−3714
−3101
−268
−2218
−754
−2274
−1465
−1906
−3315
−3861
−2859
708



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


687(L)
−2374
−1911
−4667
−4149
−624
−4356
−3248
1959
−3860
2621
550
−4014
−3844
−3100
−3584
−3637
−2284
−16
−2252
−2226
709



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


688(F)
−2145
−1754
−4444
−3835
2481
−3940
−2678
1114
−3528
2066
2253
−3582
−3521
−2731
−3201
−3119
−2036
−444
−1859
−1796
710



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


689(K
−770
−1778
−879
−243
−2063
−1733
−251
850
2294
−1721
−926
−452
−1801
148
1146
500
−677
−1382
−1948
−1464
711



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


690(L)
−2871
−2457
−4231
−4103
−1033
−3803
−3165
−541
−3734
3130
−31
−3935
−3797
−3286
−3484
−3713
−2869
−1136
−2394
−2220
712



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


691(Q
−506
−1867
−390
1178
−2181
−1467
1538
−1890
436
−1856
1294
−116
−1554
1790
1511
−401
−435
−1488
−2030
−1407
713



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


692( )
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
714



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


693(N
911
−1243
−1049
−908
−2750
−1283
−1224
−2483
−1070
−2619
−1762
2307
−1836
−911
−1451
2263
−707
−1785
−2854
−2361
715



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


694(E)
462
−1884
883
1229
−2200
−1380
−99
−1941
284
−1912
−1007
−31
1086
347
−229
391
628
−1510
−2102
−1423
716



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


695(K
472
−1945
816
942
−2258
507
−83
−2010
1429
−1957
−1042
−12
−1504
1244
−204
−340
−412
−1564
−2129
−1439
717



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


696(K
725
−1680
−306
1306
355
−1399
−79
−1633
1414
−1671
−800
−73
−1499
350
−177
548
−349
−1268
−1917
−1284
718



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−195
−7108
−3069
−894
−1115
−701
−1378
*
*


697(H
−1103
−2714
2043
320
−2881
−1429
2588
−2787
−470
−2720
−1925
2549
−1819
−164
−1109
−872
−1112
−2328
−2864
−2024
719



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−18
−6931
−7973
−894
−1115
−1083
−922
*
*


698(P)
−1509
−2622
2222
−258
−3351
−1688
−1206
−3299
−1351
−3294
−2623
−586
3242
−916
−1988
−1364
−1649
−2782
−3270
−2663
720



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−18
−6931
−7973
−894
−1115
−505
−1760
*
*


699(H
−601
−1891
−436
1733
−2050
−1535
2121
−1882
387
−1862
−1003
−192
−1631
281
1501
−502
−527
−1509
−1978
1072
721



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


700(G
2344
−1041
−2392
−2527
−3249
2572
−2371
−2970
−2636
−3255
−2405
−1721
−2031
−2309
−2645
−661
−858
−2002
−3433
−3246
722



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


701(L)
−2642
−2168
−4788
−4235
−465
−4513
−3241
96
−3878
2891
2122
−4166
−3888
−3023
−3544
−3807
−2521
−607
−2140
−2183
723



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


702(S)
−532
−1511
−753
−320
−2262
−1447
−524
−1930
1366
−2008
−1173
−494
−1732
−137
−413
1983
1524
−1495
−2248
−1710
724



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


703(D
−1718
−3250
3403
−27
−3066
−1736
2667
−3486
−1211
−3381
−2732
−400
−2240
−752
−1917
−1439
−1784
−3023
−3204
−2299
725



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


704(E)
1165
−2746
1435
2588
−3046
−1525
−639
−2845
−567
−2796
−1988
−154
−1909
−258
−1213
−943
−1174
−2380
−2996
−2179
726



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


705(E)
921
−2774
1237
2767
−3077
−1530
−662
−2877
−603
−2830
−2029
−161
−1924
−284
−1256
−967
−1205
−2412
−3032
−2209
727



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


706(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
728



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


707(D
1198
−2653
2928
116
−3164
−1523
−794
−2975
−806
−2952
−2173
1307
−1973
−435
−1474
−1014
−1273
−2473
−3161
−2340
729



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


708(N
497
−2045
1492
265
−2357
−1412
1608
−2108
128
−2074
−1183
1928
−1592
229
−403
517
−553
−1673
−2265
−1565
730



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


709(Y
−1550
−1282
−3519
−3004
−2
−3093
−1385
1232
−2635
1579
299
−2537
−2990
−2153
−2464
−2220
−1477
−195
−891
3290
731



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


710(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
732



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


711(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
733



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


712(K
921
−1810
−280
1204
−2126
−1422
−125
−1835
1451
−1838
−956
−97
−1544
311
−168
−386
1295
−1438
−2048
−1407
734



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


713(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
735



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


714(K
−1441
−2413
−1657
−802
−3025
−2193
−410
−2523
2846
−2335
−1581
−885
−2213
14
1863
−1343
790
−2218
−2320
−2022
736



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


715(P)
−540
−1180
−1850
−1974
−3103
−1352
−2072
−2962
−2149
−3170
−2355
−1527
3509
−1943
−2279
1382
−938
−2068
−3276
−2949
737



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


716(V
−1751
−1297
−4323
−3962
−1768
−4044
−3741
2446
−3832
−620
−545
−3721
−3872
−3691
−3908
−3361
−1744
2965
−3270
−2823
738



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


717(H
−792
−632
−2808
−2210
−439
−2360
2159
1209
−1870
1529
409
−1883
−2368
−1554
−1794
−1446
−733
1579
−1121
−776
739



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


718(F)
−3342
−2776
−4026
−4232
4354
−3545
−1431
−2315
−4038
−1801
−1900
−3299
−3780
−3350
−3645
−3490
−3420
−2566
−739
349
740



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


719(A
2895
−1250
−1253
−1325
−2863
−1348
−1637
−2577
−1616
−2833
−2042
1775
−1991
−1424
−1888
−716
−888
−1873
−3053
−2611
741



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


720(Y
−3687
−2724
−4232
−4508
3154
−4089
−368
−2628
−4071
−2023
−2062
−2751
−3956
−2869
−3483
−3324
−3551
−2761
3099
3684
742



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


721(H
−3205
−3079
−2723
−2890
−2110
−3046
5295
−4135
−2617
−3813
−3561
−2886
−3482
−2833
−2620
−3291
−3356
−3895
−2397
−1681
743



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


722(G
−459
−1100
−1991
−2176
−3299
3009
−2249
−3146
−2470
−3360
−2491
−1586
−2028
−2146
−2555
1630
−885
−2108
−3464
−3207
744



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


723(Y
−3590
−2700
−4146
−4380
2110
−4028
−403
−2517
−3963
−1928
−1973
−2744
−3921
−2844
−3431
−3284
−3474
−2669
338
4419
745



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


724(E)
930
−1529
−450
1712
−1762
−1469
−180
−1376
198
−1516
−699
−206
−1582
213
806
−430
−409
672
−1834
−1256
746



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


725(D
−696
−2211
2006
276
−2503
−1435
1781
−2271
47
−2216
−1327
1502
621
1163
−498
−540
−655
−1822
−2394
−1669
747



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


726(M
−522
−1463
1325
1144
−1558
−1519
−266
−1159
3
493
2326
−260
−1645
90
−458
−522
−468
−941
−1762
−1204
748



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


727(I)
−1755
−1312
−4289
−3853
−1390
−3961
−3331
2559
−3677
1028
−208
−3600
−3736
−3367
−3647
−3222
−1724
2382
−2800
−2488
749



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


728(Q
−1019
−2299
−664
1508
−2732
−1794
−307
−2378
502
−2248
−1420
−437
−1902
2305
2189
−887
−914
−1997
−2316
−1812
750



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


729(G
676
−1581
1006
−319
−2686
1982
−822
−2430
−639
−2491
−1630
−507
−1785
−454
−1135
1417
−738
−1837
−2718
−2108
751



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


730(L)
−2364
−1901
−4660
−4144
−630
−4349
−3246
2015
−3856
2597
544
−4006
−3841
−3101
−3583
−3629
−2275
2
−2255
−2227
752



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


731(F)
−2056
−1668
−4299
−3752
3006
−3840
−2396
1861
−3446
1502
554
−3415
−3499
−2733
−3168
−3023
−1964
−161
−1660
−1259
753



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


732(F)
−3581
−2688
−4165
−4401
3865
−4034
−401
−2491
−3981
−1903
−1944
−2746
−3921
−2847
−3438
−3284
−3462
−2647
341
3054
754



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


733(D
−1066
−2664
2593
230
−2949
899
−545
−2751
996
−2680
−1846
1209
−1852
−148
−1001
−857
−1060
−2284
−2863
−2068
755



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


734(R
−2957
−3022
−3318
−2735
−3796
−2998
−1968
−3912
−846
−3631
−3157
−2611
−3280
−1724
4056
−3026
−2913
−3650
−3096
−3185
756



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


735(H
673
−1400
−855
−643
−2108
−1470
3344
−1923
−596
−2073
−1302
−744
2404
−583
−931
−699
−761
−1503
−2291
−1733
757



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


736(N
−1426
−2353
−948
−735
−2920
−1991
−796
−2769
68
−2666
−1942
3441
−2268
−432
1742
−1361
−1415
−2383
−2592
−2147
758



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


737(H
−1168
−1729
−1567
−889
−1360
−2118
3917
−1446
284
543
−865
−957
−2175
−247
1258
−1239
−1055
−1304
−1577
−976
759



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


738(D
−1353
−3032
2615
202
−3188
−1564
2079
−3118
−756
−3034
−2267
2486
−1998
−383
−1443
−1092
−1377
−2642
−3178
−2294
760



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−812
−7108
−1241
−894
−1115
−701
−1378
*
*


739(N
−667
−1675
−384
−20
−2103
−1394
−82
−1850
715
−1813
−1049
2293
−1591
303
2121
−603
−621
−1504
−1871
−1383
761



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−27
−6323
−7365
−894
−1115
−292
−2448
*
*


740(F)
835
−719
−3036
−2438
1835
−2475
−1354
332
−2101
1627
473
−2059
−2472
−1719
−1973
−1574
−844
1081
−1167
−839
762



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


741(H
501
−1345
−519
3
−1474
−1478
2277
−1148
102
−1306
−513
945
−1582
159
−343
−433
1418
−890
−1646
876
763



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


742(V
−1752
−1297
−4324
−3962
−1768
−4045
−3742
2459
−3833
−621
−545
−3722
−3873
−3691
−3909
−3362
−1744
2956
−3271
−2823
764



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


743( )
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
765



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


744(H
−991
−1830
−1052
−504
−1930
−1886
3890
−1681
1542
−1767
−1038
−666
−1989
−60
176
−985
−903
206
−1921
−1408
766



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


745(G
−494
−1130
−1974
−2194
−3326
3228
−2287
−3191
−2529
−3407
−2546
−1609
−2055
−2198
−2601
938
−923
−2148
−3486
−3235
767



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


746(Y
−3482
−2868
−3701
−3919
238
−3552
−1112
−3000
−3638
−2516
−2526
−3027
−3772
−3101
−3341
−3418
−3527
−3071
−441
4711
768



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


747(R
−1317
−2448
−1232
1501
−3004
−2062
−362
−2555
2088
−2353
−1567
−710
−2107
70
2409
−1189
−1156
−2213
−2342
−1973
769



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


748(E)
−2641
−3308
−896
3732
−3966
−2458
−2043
−4105
−2128
−4016
−3555
−1531
−2959
−1842
−2560
−2479
−2750
−3722
−3563
−3385
770



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


749(E)
−603
−2088
932
1740
−2382
−1414
−194
−2138
145
−2093
−1197
1660
−1589
1157
−389
−462
−556
226
−2276
−1569
771



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


750(G
−2594
−2690
−3304
−3623
−4328
3747
−3462
−4761
−3953
−4671
−4212
−3320
−3352
−3748
−3779
−2839
−2981
−4004
−3668
−4222
772



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


751(D
−1242
−2482
2856
−92
−3324
−1544
−1056
−3220
−1180
−3222
−2479
−411
−2078
−735
−1856
2119
−1401
−2625
−3370
−2572
773



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


752(I)
−1125
−1077
−3099
−2749
−1383
−2564
−2209
2720
−2446
−620
−388
−2395
−2828
−2300
−2481
−1819
2258
661
−2292
−1909
774



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


753(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
775



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


754(T)
−1213
−1674
−2755
−2906
−3163
−1922
−2659
−2698
−2788
−3105
−2612
−2311
−2600
−2708
−2753
−1463
3819
−2197
−3286
−3156
776



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


755(P)
−2931
−2878
−3420
−3706
−4181
−2925
−3468
−4621
−3859
−4490
−4165
−3491
4225
−3781
−3695
−3182
−3279
−4087
−3594
−4064
777



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


756(F)
−3533
−2667
−4143
−4362
4034
−4011
−413
−2435
−3947
−1856
−1895
−2743
−3904
−2837
−3418
−3264
−3419
−2596
327
2564
778



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


757(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
779



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


758(M
−1664
−1479
−3731
−3304
−822
−3209
−2514
381
−2903
307
4447
−2987
−3235
−2579
−2815
−2469
−1688
1155
−2108
−1854
780



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


759(R
−841
−995
−1971
−1321
−932
−2129
−856
−136
−533
−467
2304
−1291
−2194
−758
2401
−1211
−783
1416
−1446
−1079
781



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


760(V
−954
−916
−2489
−1863
−806
−2402
−1212
292
−1157
906
98
−1725
−2437
−1258
1435
−1506
−901
2327
−1472
−1115
782



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


761(V
−1831
−1468
−4142
−3585
−674
−3694
−2641
526
−3292
1920
2093
−3318
−3425
−2728
−3099
−2876
−1760
2327
−2028
−1893
783



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


762(N
−2171
−2655
−1458
−1748
−3334
−2364
−2267
−3943
−2365
−3936
−3437
4205
−2932
−2205
−2608
−2224
−2439
−3392
−3253
−2909
784



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


763(E)
−447
−1916
638
1762
−2239
−1385
1510
−1986
1083
−1928
−1009
−19
−1493
1244
466
−323
−386
−1540
−2092
−1413
785



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


764(M
−1749
−1382
−4134
−3576
−706
−3632
−2595
1821
−3291
1705
2738
−3271
−3384
−2732
−3086
−2809
−1680
1590
−2012
−1860
786



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


765(D
−1369
−2674
3374
−58
−3453
−1571
−1095
−3380
−1289
−3368
−2659
−394
−2124
−784
−2016
1149
−1529
−2785
−3505
−2662
787



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


766(R
−2957
−3022
−3318
−2735
−3796
−2998
−1968
−3912
−846
−3631
−3157
−2611
−3280
−1724
4056
−3026
−2913
−3650
−3096
−3185
788



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


767(Y
−3616
−2704
−4178
−4426
3471
−4049
−394
−2531
−4004
−1934
−1979
−2749
−3932
−2854
−3451
−3298
−3493
−2683
349
3677
789



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


768(H
274
−2294
1079
1119
−2578
−1451
3282
−2348
−50
−2296
−1419
1227
−1687
101
−610
−606
−736
−1901
−2481
−1745
790



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


769(L)
−2092
−1719
−4323
−3846
−712
−3925
−2989
451
−3531
2641
428
−3624
−3640
−2952
−3339
−3191
−2044
1542
−2221
−2096
791



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


770(A
2058
−944
−1182
−683
−1304
−1522
−653
−783
−528
−1127
−414
−754
−1773
942
−860
−602
852
724
−1630
−1185
792



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


771(K
1238
−1887
−1224
−813
−2786
−1805
−755
−2335
3008
−2384
−1637
−897
−2102
−378
−48
−1055
−1089
−1935
−2512
−2122
793



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−169
−7108
−3273
−894
−1115
−701
−1378
*
*


772(A
1327
−1917
950
1094
−2220
−1330
−74
−1968
946
−1930
−1028
32
−1480
369
−228
547
−411
−1533
−2115
−1424
794



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−6956
−7998
−894
−1115
−1036
−964
*
*


773(A
3386
−1299
−2601
−2766
−3046
−1559
−2499
−2553
−2749
−2990
−2405
−2021
−2272
−2539
−2697
−1037
−1201
−1940
−3200
−3073
795



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−6956
−7998
−894
−1115
−525
−1714
*
*


774(I)
−1087
−861
−3107
161
−882
−2803
−1771
2342
−2281
1091
78
−2300
−2777
−2009
−2269
−1916
−1039
1702
−1691
−1345
796



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


775(Q
375
−1755
−273
815
−2036
−1376
−39
−1756
367
−1753
−857
1007
−1469
1244
843
−290
959
12
−1964
−1309
797



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


776(M
1052
−610
−1546
−994
−696
582
−640
−244
−804
810
1789
−967
−1833
−639
−1021
395
−384
−113
−1120
−726
798



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


777(I)
−416
−776
−1113
474
−802
469
−424
1231
−400
255
48
−674
−1747
951
−718
−659
−357
665
−1177
−745
799



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


778(D
1034
−2035
2294
−106
−2869
−1459
−830
−2614
−751
−2671
−1863
−387
2260
−474
−1320
−825
−1014
−2092
−2906
−2215
800



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


779(A
1299
−1548
−409
909
−1800
43
−141
−1467
231
−1558
−718
−156
−1540
264
1153
−374
−369
158
−1848
−1253
801



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1040
−7108
−981
−894
−1115
−701
−1378
*
*


780(F)
−585
−915
−883
−659
2573
−1541
−52
−591
−624
−695
−233
1803
−1767
−468
−869
−739
−609
−487
−70
917
802



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−424
−6099
−2060
−894
−1115
−1914
−445
*
*


781(N
−313
−1042
254
152
−1409
−814
−286
−1511
−122
−1692
−1083
3011
−1287
−82
−434
−340
−474
−1140
−1574
−976
803



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−41
−5717
−6759
−894
−1115
−2091
−386
*
*


782(E)
−647
−1563
589
2680
−1901
−941
−188
−1630
4
−1762
−1176
156
−1345
101
−395
−544
−693
−1348
−1863
−1374
804



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−41
−5717
−6759
−894
−1115
−2091
−386
*
*


783(K
−682
−1365
−434
−118
−1773
−1231
−19
−1449
2744
−1499
−893
−244
−1470
310
740
−670
−643
−1208
−1538
−1198
805



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−41
−5717
−6759
−894
−1115
−612
−1532
*
*


784(Y
−620
−433
−2763
−2164
−265
−2214
−1076
1484
−1822
144
2036
−1779
−2223
−1475
−1707
−1306
−562
1635
−935
2216
806



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−20
−6788
−7830
−894
−1115
−1306
−747
*
*


785(K
−540
−1908
−286
1554
−2271
235
−21
−1969
1597
−1892
−1016
−65
−1527
423
1275
−418
−461
−1559
−2023
−1427
807



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−20
−6788
−7830
−894
−1115
−421
−1984
*
*


786(D
−495
−1834
1772
243
−2095
−1409
−135
−1811
226
93
−952
959
−1543
1255
−279
−392
713
−1426
−2056
−1398
808



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


787(K
−1474
−2433
−1703
−833
−3058
−2216
−418
−2547
2890
−2352
−1602
−908
−2233
6
1874
−1375
633
−2246
−2329
−2042
809



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


788(H
−451
−1596
−374
131
−1721
4
2032
−1490
223
−1570
−735
−150
−1554
1294
−242
993
−394
−1170
−1803
1657
810



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


789(A
2417
−1302
−925
−642
−2356
−1358
−885
−2038
−600
−2179
−1358
−718
−1792
1772
−969
686
−646
−1531
−2461
−1952
811



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


790(E)
1699
−2717
1896
1966
−3018
−1521
−628
−2814
−547
−2768
−1958
−152
−1900
−245
−1190
−928
−1153
−2352
−2970
−2159
812



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


791(F)
−576
−843
−1351
18
2111
−1835
−543
−341
927
1121
58
−880
−1903
−468
−730
−859
−511
−255
−1145
−680
813



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


792(C
−1112
2697
−3476
−2950
−1103
−2900
−2047
2026
−2659
−523
−143
−2556
−2914
−2378
−2583
−2056
623
2331
−1900
−1524
814



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


793(Q
419
−1959
511
264
−2287
−1397
−160
−2033
201
−2001
−1102
892
−1555
2127
−318
1297
−485
−1597
−2190
−1503
815



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


794( )
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
816



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


795(E)
−617
−2049
−177
2132
−2369
−1467
1606
−2104
1334
−2049
−1159
−104
−1611
267
−171
468
−558
−1677
−2212
−1550
817



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


796(M
−2288
−1880
−4481
−3899
1874
−4066
−2635
72
−3594
1748
3848
−3656
−3608
−2775
−3272
−3259
−2174
−566
−1778
−1555
818



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


797(D
−1194
−2847
2837
1238
−3100
−1527
−634
−2920
−571
−2845
−2036
1124
−1913
−251
−1231
−958
827
−2447
−3037
−2200
819



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


798(H
−831
−2392
1999
980
−2678
−1463
2287
−2459
551
−2391
−1516
1798
−1718
62
−667
−653
−799
−2000
−2566
−1815
820



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


799(M
−496
−1070
−899
−350
1464
−1648
−334
−716
1384
−934
2286
1246
−1730
−100
−455
−629
−434
−548
−1346
−826
821



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


800(I)
−1200
−1080
−2894
−2252
−618
−2712
−1476
2020
−1547
1701
361
−2078
−2664
−1554
1445
−1820
−1129
84
−1503
−1217
822



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


801(Q
1195
−1999
1275
287
−2307
−1399
−132
−2058
888
−2010
−1104
1022
−1544
1594
−271
−396
−475
−1616
−2187
−1494
823



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


802(E)
−464
−1834
−313
1601
−2133
−1426
−72
−1849
1475
−301
−936
−76
−1522
1237
−64
−359
488
−1446
−2019
−1377
824



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


803(H
1285
−1168
−1100
−753
−1895
−1421
3671
−1567
−616
−1783
−1019
−796
−1815
−585
−942
−599
1325
−1196
−2122
−1628
825



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


804(H
−457
−1111
−796
−240
1415
−1595
1499
−796
−56
−1014
−275
1464
−1677
−16
1113
−558
−395
539
−1363
−816
826



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


805(Q
961
−1898
742
284
−2209
−1388
1553
−1951
1291
−1907
−995
−26
−1499
1771
−144
−331
−392
−1516
−2082
−1407
827



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


806(Y
−3616
−2706
−4170
−4415
2670
−4045
−396
−2536
−3994
−1940
−1985
−2748
−3931
−2852
−3447
−3296
−3495
−2687
347
4234
828



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


807(I)
1201
−821
−2746
−2313
−1223
−2126
−1705
2731
−2058
−749
−304
−1944
−2428
−1844
−2105
−1330
893
505
−1890
−1524
829



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


808(R
−841
−995
−1971
−1321
−932
−2129
−856
−136
−533
−467
2304
−1291
−2194
−758
2401
−1211
−783
1416
−1446
−1079
830



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


809(D
−1157
−2811
2313
1149
−3071
−1519
−606
−2891
−523
−2811
−1992
1715
−1893
−218
−1175
1334
−1159
−2414
−2998
−2166
831



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


810(N
377
−1890
−201
941
−2201
−1398
−93
−1936
1328
−1904
−1001
1972
−1518
353
−173
−356
594
−1511
−2089
−1420
832



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


811(G
−1501
−2131
−1258
−1392
−2600
3138
2851
−3219
−1608
−3233
−2576
−1477
−2541
−1589
−1850
−1578
−1737
−2674
−2756
−2168
833



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


812(Y
385
−1523
−449
96
−1722
−1461
−132
−1384
959
−1486
−659
−175
−1554
1557
−186
−399
1250
−1083
−1786
1631
834



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


813(D
−2784
−3432
4016
−1200
−4140
−2466
−2197
−4505
−2621
−4365
−3956
−1551
−3014
−2039
−3232
−2593
−2938
−4046
−3710
−3552
835



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


814(L)
−587
−625
−1931
−1364
−454
−1983
1655
818
−1068
1666
322
−1268
955
−903
−1182
−1033
−529
21
−1001
−599
836



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


815(P)
−1667
−2676
−329
1564
−3405
−1913
−1351
−3268
−1273
−3276
−2612
−852
3489
−1057
−1736
−1555
−1787
−2810
−3275
−2761
837



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


816(E)
−1715
−3562
2447
2878
−3759
−1632
−991
−3691
−1235
−3569
−2910
−235
−2166
−663
−2079
−1378
−1784
−3173
−3732
−2750
838



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


817(V
−537
−374
−2717
−2103
914
−2090
−949
1111
−1742
−147
443
−1675
−2132
−1398
−1608
−1173
1109
1932
−845
1322
839



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


818(T)
−442
−1699
−325
943
−1942
−1425
−95
−1640
311
−1680
−816
906
−1525
330
784
−363
1592
−1283
−1922
1369
840



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


819(D
−1684
−3395
2909
109
−3677
−1635
−1030
−3669
−1283
−3567
−2912
2851
−2178
−713
−2110
−1377
−1776
−3137
−3688
−2726
841



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


820(W
−2023
−1815
−3480
−3119
26
−3133
−1280
−780
−2462
−329
2687
−2604
−3188
−2264
−2350
−2462
−2001
−1067
5349
158
842



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


821(Q
−408
−1655
−342
851
−1897
−1411
−76
−1588
323
−1635
−770
−87
785
1202
869
−334
619
752
−1887
−1265
843



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


822(W
−2077
−1892
−3023
−2742
805
−3065
−450
−1653
−2397
−1546
−1211
−2106
−3118
−1977
−2345
1002
−2038
−1614
4785
2862
844



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


823(K
−479
−1638
−472
91
1024
−1483
−125
−1548
1811
−1610
−769
−180
147
288
950
516
−413
−1222
−1868
−1282
845



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7108
−8150
−894
−1115
−701
−1378
*
*


824(G
−736
−2137
965
180
−2586
1581
−401
−2350
−145
−2321
−1448
1563
712
15
−699
528
−738
−1882
−2516
−1799
846



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−276
−7108
−2582
−894
−1115
−701
−1378
*
*


825(L)
−1714
−1375
−3901
−3421
−618
−3530
−2616
734
−3109
2342
497
−3185
−3329
−2647
−2982
−2766
−1674
1950
−2019
−1825
847



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6850
−7893
−894
−1115
−1215
−813
*
*


826(K
−1321
−2321
−787
−481
−2892
−1879
−447
−2552
3186
−2406
−1676
717
−2074
−54
419
−1212
−1230
−2209
−2369
−1979
848



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1043
−6850
−983
−894
−1115
−1215
−813
*
*


827(V
11
−281
−1388
−1014
−772
−1145
−782
672
−779
−271
145
−834
−1527
−694
−967
−363
1648
1687
−1388
−977
849



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


828(D
−878
−1818
3239
428
−2222
−972
−438
−2180
−575
−2276
−1711
95
−1458
−189
−1148
−733
−979
−1822
−2152
−1652
850



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


829(K
−453
−1667
328
1484
−2007
−1088
173
−1659
1862
−1641
−861
236
−1304
570
510
−325
−390
−1317
−1793
−1239
851



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


830(K
−207
−1058
−256
150
−1533
−1073
83
−1043
1817
−1222
−493
−14
−1295
442
505
−228
1229
−760
−1520
−1048
852



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


831(G
414
−272
−462
−426
−1782
1704
−635
−1518
−577
−1737
−936
−257
−1039
−416
−850
1592
98
−853
−1974
−1547
853



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


832(M
1337
−339
−1188
−761
−497
−1179
−525
361
−511
26
2356
−679
−1477
−431
−716
−350
−116
415
−1109
−709
854



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


833(V
−753
−590
−2446
−1973
−226
−2358
−1433
1299
−1657
1429
779
−1837
−2356
−1469
−1726
−1538
−740
2084
−1336
−966
855



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


834(T)
413
−229
−707
−500
−1469
−500
−543
−1022
−421
−1349
−599
−312
−1063
−330
−674
1483
1911
−534
−1729
−1303
856



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


835(A
2597
−310
−1033
−1004
−1499
−618
−950
−768
−944
−1290
−768
−669
−1235
−852
−1081
−19
−107
−412
−1828
−1482
857



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


836(T)
93
−414
−954
−868
−1353
−741
−817
−616
−701
−1116
−632
−643
−1304
−696
−854
−136
2822
−336
−1700
−1317
858



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


837(A
2597
−310
−1033
−1004
−1499
−618
−950
−768
−944
−1290
−768
−669
−1235
−852
−1081
−19
−107
−412
−1828
−1482
859



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


838(A
2597
−310
−1033
−1004
−1499
−618
−950
−768
−944
−1290
−768
−669
−1235
−852
−1081
−19
−107
−412
−1828
−1482
860



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


839(T)
93
−414
−954
−868
−1353
−741
−817
−616
−701
−1116
−632
−643
−1304
−696
−854
−136
2822
−336
−1700
−1317
861



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


840(A
2597
−310
−1033
−1004
−1499
−618
−950
−768
−944
−1290
−768
−669
−1235
−852
−1081
−19
−107
−412
−1828
−1482
862



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


841(G
−461
−838
−976
−1119
−2120
3148
−1219
−2101
−1326
−2273
−1700
−986
−1505
−1212
−1430
−653
−781
−1558
−1923
−1928
863



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


842(D
−878
−1818
3239
428
−2222
−972
−438
−2180
−575
−2276
−1711
95
−1458
−189
−1148
−733
−979
−1822
−2152
−1652
864



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


843(N
−431
−1158
164
47
−1545
−910
−407
−1680
−257
−1848
−1242
3201
−1391
−207
−570
−456
−599
−1296
−1696
−1106
865



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−2039
−402
*
*


844(E)
−771
−1700
518
2837
−2056
−1033
−298
−1811
−123
−1925
−1338
65
−1445
−9
−532
−658
−819
−1519
−1994
−1511
866



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−38
−5845
−6888
−894
−1115
−238
−2716
*
*


845( )
−30
439
11
363
−394
−739
699
−414
463
−757
501
286
−577
639
165
−23
111
−312
697
−25
867



*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*



*
*
*
*
*
*
*
*
0


















TABLE 7









HMMER2.0 [2.3.2]



NAME XFP N



ACC PF09364.1



DESC XFP N-terminal domain



LENG 396



ALPH Amino



RF no



CS no



MAP yes



COM hmmbuild -f -F HMM fs.ann SEED.ann



COM hmmcalibrate—seed 0 HMM fs.ann



NSEQ 6



DATE Thu May 3 17:57:17 2007



CKSUM 7893



GA 15.1 15.1



TC 15.1 15.1



NC 14.6 14.6



XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4



NULT −4 −8455



NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644



EVD −10.948357 0.672333

































A
C
D
E
F
G
H
I
K














HMM
m->m
m->i
m->d
i->m
i->i
d->m
d->d
b->m
m->e
L
M
N
P
Q
R
S
T
V
W
Y






−193
*
−3000


1(K)
−1968
−2951
−2155
−1320
−3390
−2747
−966
−3007
2330
−2844
−2086
−1422
2165
−549
1590
−1872
−1791
−2712
−2821
1937
1



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−1193
−9626


2(I)
−1769
−1476
−4090
−3550
−1526
−3579
−2618
2387
−3249
1441
−544
1396
−3532
−2933
−3155
−2723
−1726
1550
−2406
−2063
2



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9624


3(S)
−1378
−2318
1402
−1145
−3726
−2032
−1821
−3503
−1805
−3583
−2748
−1309
−2587
−1497
−2321
2452
2280
−2794
−3796
−3178
3



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9622


4(D)
−2415
−4334
2560
2263
−4523
−2295
−1677
−4467
−1966
−4327
−3682
−889
2279
−1352
−2856
−2064
−2494
−3929
−4515
−3478
4



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9620


5(E)
818
−2294
−768
2387
−2689
−1890
−629
−2403
−181
−2389
−1504
−596
−2035
−193
1173
−879
979
−1984
−2580
−1944
5



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9618


6(Y)
868
−2568
−747
2575
−2297
−2202
−1201
−2582
−1098
−2680
−1956
−1001
−2523
−938
−1573
−1484
−1571
−2270
−2610
2844
6



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9617


7(L)
−2772
−2280
−5308
−4845
−1620
−5006
−4218
1675
−4673
2505
−404
−4676
−4560
−4047
−4494
−4304
−2713
1658
−3269
−3192
7



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9615


8(H)
899
−2380
−623
1169
−2703
−1834
2073
−2447
1918
−2389
−1478
1301
−1952
−64
−563
−794
−860
−2005
−2553
−1878
8



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9613


9(K)
−1161
−2683
1251
−212
−2990
727
−723
−2756
1972
−2690
−1794
1663
−2115
1586
−911
−1006
−1116
−2301
−2857
−2139
9



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9611


10(M)
−2709
−2269
−5162
−4623
−1349
−4724
−3702
1512
−4374
1792
3396
−4390
−4320
−3675
−4116
−3951
−2630
1553
−2866
−2812
10



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9609


11(H)
−2820
−4339
3411
−925
−4078
−2615
3698
−4807
−2416
−4617
−4088
−1308
−3181
−1809
−3221
−2491
−2928
−4293
−4210
−3302
11



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9607


12(K)
1531
−2609
−1521
−881
−3185
677
−855
−2809
2197
−2705
−1875
−1069
−2428
−423
1698
−1376
−1380
−2418
−2783
−2320
12



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9605


13(Y)
−4639
−3574
−5042
−5388
3053
−4919
−1116
−3531
−4943
−2851
−2938
−3536
−4775
−3673
−4311
−4166
−4491
−3684
3607
3791
13



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9604


14(W)
−3826
−3700
−3776
−3690
−1600
−3954
−2357
−4085
−2609
−3642
−3464
−3450
−4249
2161
−2584
−3858
−3853
−4045
5840
−1155
14



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9602


15(R)
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
15



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9600


16(A)
3208
−1704
−3999
−4070
−3276
−2242
−3400
−1516
−3824
−2767
−2340
−2844
−2960
−3485
−3660
−1604
−1654
1301
−3916
−3651
16



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9598


17(C)
3231
3331
−4290
−4524
−4051
−2061
−3603
−3558
−4177
−4038
−3241
−2842
−2850
−3725
−3864
−1443
−1636
−2656
−4310
−4227
17



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9596


18(N)
−2093
−3624
1842
−565
−4191
−2225
−1624
−4071
−1786
−4008
−3287
3436
−2749
−1290
−2544
−1859
1312
−3511
−4199
−3291
18



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9594


19(Y)
−4605
−3570
−5021
−5357
2115
−4893
−1140
−3492
−4921
−2816
−2906
−3546
−4765
−3679
−4305
−4160
−4468
−3656
−389
4545
19



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9592


20(L)
−3485
−2916
−5860
−5269
−1134
−5582
−4270
1416
−5040
2720
2646
−5288
−4721
−3890
−4562
−4907
−3336
−1219
−2912
−3094
20



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9591


21(A)
2093
−1633
−3496
−3406
−3943
1228
−3043
−3705
−3256
−3935
−3021
−2418
−2652
−2942
−3281
2007
1343
−2649
−4148
−3944
21



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9589


22(I)
1858
−1782
−4736
−4347
−2337
−4240
−3903
2415
−4179
−1341
−1167
−4002
−4207
−4002
−4206
−3513
−2172
2258
−3603
−3167
22



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9587


23(G)
−1257
−1874
−3207
−3523
−4341
3341
−3408
−4248
−3853
−4463
−3557
−2605
−2866
−3402
−3760
1410
−1718
−3031
−4475
−4358
23



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9585


24(M)
−2521
−2386
−3846
−3279
−1155
−3984
−2657
−686
−2594
1335
3429
−3250
−3784
3078
−2603
−3140
−2428
−1237
−2461
−2296
24



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9583


25(I)
−2891
−2390
−5370
−4957
−1628
−5096
−4334
3554
−4751
1008
−437
−4806
−4650
−4141
−4574
−4462
−2842
−63
−3311
−3180
25



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9581


26(Y)
−4614
−3571
−5028
−5366
3123
−4901
−1136
−3497
−4929
−2819
−2909
−3545
−4768
−3679
−4308
−4163
−4474
−3661
−384
4150
26



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9579


27(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4358
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
27



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9577


28(R)
−1752
−2663
−1994
−1206
−3071
−2612
−927
−2620
1567
1194
−1815
−1326
−2642
−523
2318
1048
−1606
−2354
−2690
−2328
28



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9576


29(D)
−2163
−3991
2853
1466
−4198
−2225
−1494
−4086
−1627
−3968
−3237
1901
−2710
−1142
−2410
971
−2206
−3573
−4159
−3203
29



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9574


30(N)
−2100
−2812
−1725
−2071
−4541
1400
−2845
−4689
−3101
−4717
−3995
3948
−3237
−2675
−3479
−2189
−2459
−3750
−4448
−4114
30



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9572


31(P)
−2845
−2996
−4029
−4118
−2821
−3470
−3585
−2547
−3827
1069
−2145
−3734
3854
−3778
−3737
−3186
−3115
−2756
−3564
−3278
31



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9570


32(L)
−3571
−3027
−4990
−4839
−362
−4751
−2053
−1386
−4381
2878
−762
−3969
−4502
−3586
−4013
−4093
−3476
−1986
−1288
2490
32



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9568


33(M)
−2211
−1924
−4481
−3865
1786
−3872
−2670
−469
−3517
2158
2560
−3505
−3670
−2910
−3263
−3004
1361
−903
−2139
−1963
33



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1891
−7944
−464
−894
−1115
−701
−1378
−9818
−9566


34(S)
87
−526
−885
−918
−1797
−699
−997
−1679
−970
−1948
−1267
−663
−1335
−874
−1147
2826
−252
−1082
−2036
−1577
34



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−34
−6087
−7129
−894
−1115
−2832
−218
−9818
−9564


35(V)
−734
−692
−2391
−2193
−909
−2005
−1781
1094
−1922
−136
−10
−1902
−2325
−1852
−1974
−1413
−858
2960
−1857
−1405
35



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−34
−6087
−7129
−894
−1115
−2832
−218
−9818
−9562


36(T)
−84
−586
−1220
−1166
−1618
−901
−1090
−915
−1004
−1405
−917
−886
−1488
−990
−1134
−317
3114
−605
−1941
−1587
36



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−34
−6087
−7129
−894
−1115
−133
−3510
−9818
−9560


37(R)
−2583
−3338
−2968
−1938
−4226
2024
−1216
−3597
1648
−3282
−2594
−1884
−3156
−798
2920
−2479
−2342
−3324
−3144
−3007
37



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9558


38(E)
−1678
−2848
−628
2969
−3815
1219
−1598
−3602
−1552
−3614
−2817
−1072
−2634
−1251
−2123
−1592
1327
−3012
−3808
−3091
38



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9557


39(P)
−3058
−3915
1871
−1726
−4973
−2986
−2783
−5207
−3189
−5075
−4546
−2051
3849
−2577
−3857
−2918
−3276
−4561
−4629
−4315
39



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9555


40(L)
−3389
−2846
−5732
−5157
1795
−5405
−3964
1554
−4913
2673
66
−5067
−4642
−3819
−4457
−4697
−3249
−1211
−2753
−2720
40



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9553


41(K)
−1121
−2502
−1004
1296
−2888
−2042
−588
−2583
2027
−2485
−1598
−681
−2119
1586
1299
−1000
1054
−2164
−2595
−2010
41



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9551


42(P)
784
−2052
−818
1262
−2296
−1869
−546
−1970
−134
107
−1190
−567
1773
−132
1208
−813
−814
−1637
−2305
−1705
42



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9549


43(E)
−2121
−3903
1652
3001
−4126
−2231
−1448
−3991
1462
−3872
−3127
−825
−2693
−1089
−2171
−1828
−2151
−3493
−4045
−3137
43



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9547


44(H)
−1900
−3602
2493
−392
−3825
−2165
3314
−3680
−1250
−3583
−2790
1715
−2572
1675
−1911
−1645
−1910
−3197
−3754
−2893
44



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9545


45(I)
−2547
−2059
−5152
−4751
−2029
−4889
−4388
2502
−4626
1616
−797
−4548
−4567
−4264
−4595
−4202
−2516
2260
−3637
−3383
45



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9543


46(K)
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
46



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9541


47(H)
826
−2035
−963
−419
−2397
−1898
3051
−2073
−206
−2136
−1290
−675
932
−235
1162
−866
1249
−1718
−2394
−1810
47



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9539


48(R)
−2700
−3449
−3065
−1955
−4296
−3222
−1177
−3628
1592
−3280
−2601
1619
−3187
−756
3400
−2568
−2401
−3384
−3125
−3003
48



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9537


49(L)
−2172
−1972
−4362
−3884
−1289
−3737
−2942
−312
−3536
2565
−217
−3515
1361
−3084
−3377
−2952
−2169
1219
−2509
−2319
49



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9535


50(V)
−2641
−2155
−5210
−4776
−1803
−4918
−4261
1765
−4623
2179
−584
−4583
−4545
−4128
−4515
−4219
−2597
2215
−3420
−3258
50



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9533


51(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
51



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9531


52(H)
−4836
−4275
−4356
−4629
−3663
−4242
5422
−5932
−4492
−5436
−5298
−4579
−4696
−4620
−4352
−5005
−5030
−5648
−3750
−3259
52



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9529


53(W)
−5598
−4432
−5385
−5722
−3380
−4524
−4140
−5941
−5643
−5318
−5354
−5427
−4938
−5474
−5178
−5909
−5751
−5890
6275
−2993
53



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9527


54(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
54



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9526


55(T)
−1093
−1706
−3487
−3693
−4074
−1966
−3302
−3842
−3638
−4134
−3253
−2558
−2748
−3296
−3520
1110
3646
−2760
−4304
−4125
55



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9524


56(C)
−1052
2901
−3250
−2679
−1060
−2526
−1634
1315
−2341
−866
−266
−2229
−2670
−2018
−2238
1521
1154
1349
−1596
−1246
56



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9522


57(P)
−1362
−1972
−3107
−3390
−4250
−2166
−3327
−4213
−3641
−4411
−3553
−2613
3840
−3308
−3600
1256
−1811
−3082
−4372
−4198
57



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9520


58(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
58



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9518


59(Q)
−2629
−2340
−4433
−3873
−1140
−4292
−3047
1389
−3342
2319
−20
−3746
−4000
2559
−3238
−3469
−2536
−946
−2531
−2422
59



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9516


60(S)
−1129
−1869
−2034
−2027
−3771
−1956
−2322
−3546
−2277
−3697
−2821
2528
−2607
−2062
−2583
2544
1447
−2665
−3893
−3470
60



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9514


61(F)
−3253
−2755
−5063
−4895
3979
−4679
−2230
1673
−4579
−697
−843
−4029
−4483
−3735
−4216
−4008
−3205
−1123
−1463
−453
61



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9512


62(I)
−2667
−2178
−5235
−4799
−1779
−4947
−4278
2326
−4646
2166
−559
−4613
−4558
−4130
−4529
−4251
−2621
1716
−3410
−3264
62



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9510


63(Y)
−4030
−3324
−4830
−4953
82
−4664
−1400
−2548
−4444
776
−2024
−3603
−4588
−3589
−4024
−4010
−3953
−2880
−668
4492
63



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9508


64(A)
2914
−1624
−3741
−3790
−3997
−1911
−3253
−3736
−3609
−4009
−3103
−2541
−2685
−3231
−3506
1407
1356
−2665
−4233
−4079
64



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9506


65(H)
−4836
−4275
−4356
−4629
−3663
−4242
5422
−5932
−4492
−5436
−5298
−4579
−4696
−4620
−4352
−5005
−5030
−5648
−3750
−3259
65



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9504


66(C)
868
2955
−3843
−3261
−1152
−3113
−2129
1511
−2905
1928
−237
−2799
−3137
−2521
−2739
−2241
−1449
−4
−1921
−1610
66



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9502


67(N)
−3642
−3838
−3004
−3356
−4743
−3612
−3773
−5643
−4099
−5460
−5060
4378
−4185
−3865
−4225
−3744
−3950
−5002
−4391
−4381
67



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9500


68(R)
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
68



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9498


69(I)
687
−1693
−4550
−4054
−1797
−4053
−3239
2451
−3802
1595
−714
−3702
−3939
−3479
−3713
−3246
−2027
1554
−2897
−2565
69



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9496


70(I)
−3202
−2787
−5004
−4996
−2836
−4384
−4440
3937
−4838
−1815
−1871
−4704
−4600
−4683
−4704
−4353
−3280
−603
−3938
−3661
70



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9494


71(N)
777
−2760
−1484
−853
−3260
−2383
−822
−2877
1407
−2738
−1911
2481
−2455
−385
2206
−1436
−1435
−2500
−2791
−2332
71



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9492


72(K)
−1654
−2912
1393
−667
−3173
−2287
−1055
−2863
3038
286
−2070
−954
−2512
−658
−811
−1519
−1598
−2533
−3029
−2434
72



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9490


73(Y)
−3965
−3303
−4553
−4498
2089
−4562
2685
−3175
−3880
−2696
−2630
−3283
−4469
−3249
1301
−3756
−3844
−3248
−415
3893
73



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9488


74(D)
−2647
−4396
3720
−698
−4634
−2448
−1896
−4723
−2212
−4559
−3990
−1090
−3022
1848
−3057
−2298
−2751
−4181
−4595
−3647
74



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9486


75(Q)
786
−1836
−2085
−1707
−1785
−2491
−1572
−1296
−1287
1558
−860
−1741
−2731
3104
−1481
−1664
−1471
−1312
−2370
−1975
75



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9484


76(N)
−2189
−3961
2351
−476
−4203
−2257
−1501
−4079
1323
−3955
−3231
3154
−2736
−1150
−2219
−1890
−2229
−3577
−4120
−3208
76



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9482


77(M)
−1639
−1552
−3944
−3485
−1584
−3112
−2598
−102
−3126
−813
4345
−2996
−3316
−2834
−3037
−2326
1195
1340
−2475
−2167
77



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9480


78(I)
−2541
−2130
−4989
−4447
1768
−4503
−3473
2245
−4181
1829
−210
−4162
−4181
−3554
−3947
−3708
−2469
1402
−2759
−2626
78



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9478


79(Y)
−4613
−3570
−5027
−5365
3096
−4900
−1135
−3496
−4928
−2818
−2908
−3544
−4767
−3678
−4307
−4162
−4473
−3660
−383
4168
79



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9476


80(M)
−2401
−1938
−4995
−4588
−2135
−4683
−4177
2411
−4441
−955
3356
−4340
−4447
−4169
−4437
−3971
−2375
2371
−3610
−3272
80



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9474


81(C)
−924
2595
−3097
−2552
−1083
823
−1551
−560
−2221
−963
2359
−2072
−2516
−1903
−2136
739
−963
2007
−1573
−1229
81



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9472


82(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
82



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9470


83(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
83



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9468


84(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
84



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9466


85(H)
−4836
−4275
−4356
−4629
−3663
−4242
5422
−5932
−4492
−5436
−5298
−4579
−4696
−4620
−4352
−5005
−5030
−5648
−3750
−3259
85



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9464


86(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
86



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9461


87(G)
2125
−1842
−3631
−3927
−4350
3048
−3538
−4169
−4033
−4428
−3528
−2733
−2876
−3575
−3846
−1489
−1707
−2984
−4482
−4443
87



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9459


88(P)
−2640
−3260
−2127
−2297
−4320
−2987
−2661
−4510
−2216
−4379
−3792
−2411
3576
2864
−2373
−2683
−2863
−3935
−4069
−3806
88



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9457


89(A)
2982
−1594
−3754
−3796
−3529
1052
−3162
−2818
−3604
−3423
−2664
−2573
−2719
−3219
−3468
−1317
−1463
1086
−3887
−3678
89



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9455


90(M)
−1348
−1165
−3573
−2973
1525
706
−1766
1561
−2606
−377
3758
−2527
−2921
−2210
−2439
−2009
−1294
−310
−1556
−1184
90



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9453


91(V)
−1974
−1692
−4373
−3885
−1655
−3760
−3023
236
−3586
1658
−599
−3493
−3751
−3250
−3485
−2959
1156
2628
−2723
−2419
91



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9451


92(S)
1951
−1569
−3422
−3255
−3372
1033
−2819
−2999
−3074
−3315
−2500
−2366
−2636
−2776
−3104
2094
−1382
1224
−3674
−3414
92



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9449


93(N)
1058
−2388
−785
−572
−3066
−1969
−1036
−2804
−709
−2811
−1947
2272
2144
1706
−1182
−1128
−1239
−2319
−3013
−2367
93



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9447


94(C)
−968
2761
−3158
−2743
−1848
−2096
−2020
−1352
−2456
835
−1054
−2166
−2572
−2189
−2458
2381
1213
−1124
−2305
−1969
94



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9445


95(Y)
−4608
−3570
−5023
−5360
2380
−4895
−1138
−3494
−4924
−2817
−2907
−3545
−4766
−3679
−4306
−4161
−4470
−3658
−386
4475
95



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9443


96(L)
−3428
−2867
−5794
−5267
−1216
−5534
−4333
1535
−5028
2951
−6
−5264
−4750
−3972
−4604
−4907
−3306
−978
−2993
−3105
96



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9441


97(D)
−2629
−4604
3026
2782
−4770
−2374
−1828
−4782
−2235
−4614
−4052
−973
−2960
−1523
−3217
−2241
−2730
−4225
−4757
−3689
97



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9439


98(G)
−2862
−3768
1871
−1615
−5004
3370
−2705
−5181
−3146
−5060
−4493
−1931
−3470
−2488
−3861
−2740
−3106
−4452
−4685
−4318
98



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9437


99(T)
−1078
−1695
−3486
−3659
−4077
−1954
−3276
−3853
−3599
−4130
−3239
−2539
−2733
−3255
−3500
2681
2838
−2756
−4301
−4124
99



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9435


100(Y
−3992
−3300
−4836
−4941
66
−4664
−1423
−2456
−4436
992
−1925
−3615
−4579
−3584
−4019
−4006
−3913
−2810
−690
4437
100



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9433


101(S)
−1032
−1663
−3422
−3512
−4087
1390
−3188
−3870
−3492
−4107
−3184
−2468
−2688
−3124
−3454
2496
2388
−2740
−4293
−4121
101



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9430


102(E
−1823
−3131
−875
2933
−3614
−2362
−1174
−3322
−553
−3205
−2407
−1048
1540
−771
1382
−1665
−1779
−2906
−3277
−2712
102



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9428


103(F)
863
−914
−3349
−2737
2502
−2631
−1388
−298
−2350
698
−39
−2234
−2667
−1962
−2166
−1719
−1033
1141
−1185
1869
103



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9426


104(Y
−4352
−3468
−4770
−5008
2112
−4760
−1124
−3396
−4660
−2798
−2831
1505
−4661
−3557
−4156
−4005
−4236
−3515
−387
4271
104



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9424


105(P)
1085
−2001
−3515
−3791
−4319
−2231
−3514
−4118
−3884
−4378
−3566
−2802
3892
−3541
−3758
−1662
−1872
−3068
−4416
−4363
105



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9422


106(N
−1628
−3254
1441
2076
−3527
−2085
−1091
−3331
−913
−3246
−2403
2397
−2414
−688
1516
−1414
−1613
−2859
−3417
−2622
106



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9420


107(I)
−1569
2626
−3973
−3416
−1377
−3324
−2311
2751
−3082
−941
−528
−2965
−3319
−2753
−2944
−2457
−1527
1473
−2109
2078
107



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9418


108(T
908
−1834
−1592
−1168
−2951
−1885
−1379
−2651
−1086
−2760
−1898
−1245
−2295
1915
−1496
1739
2152
−2098
−3019
−2494
108



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9416


109(M
−879
−2216
−787
1054
1375
−1857
−485
−2215
1265
−2203
1871
−512
−1943
1655
1154
−781
−811
−1824
−2400
−1773
109



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9414


110(D
−2264
−3415
3677
−1019
−4473
−2407
−2124
−4366
−2457
−4415
−3784
−1339
−3017
−1850
−3214
−2128
1551
−3724
−4492
−3732
110



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9412


111(E
−1628
−2756
−1092
2433
−3004
−2358
−986
−2615
1561
1281
−1850
−1034
−2517
−587
−533
−1527
−1543
−2348
−2851
−2339
111



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9409


112(E
800
−2932
1355
2151
−3219
−1985
−890
−3005
−643
−2935
−2058
1450
−2244
1611
−1220
−1179
−1330
−2541
−3109
−2350
112



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9407


113(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
113



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9405


114(M
−3599
−3029
−5877
−5316
−1121
−5599
−4270
−608
−5016
2422
4020
−5344
−4752
−3899
−4542
−4979
−3452
−1416
−2899
−3042
114



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9403


115(K
−1165
−2535
−1051
−455
−2930
−2079
−612
−2618
1972
−2514
−1633
1596
−2153
1677
1320
873
−1075
−2202
−2617
−2045
115



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9401


116(K
−2618
−3410
−2984
−1883
−4230
−3176
−1148
−3580
2782
−3243
−2552
1766
−3140
−725
2514
−2486
−2331
−3326
−3100
−2957
116



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9399


117(L)
−3686
−3083
−5668
−5295
2704
−5391
−3131
−837
−5017
2713
−141
−4846
−4727
−3862
−4511
−4763
−3544
−1624
−2119
−1439
117



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9397


118(F)
−2097
2719
−4399
−3936
3542
−3704
−2285
1698
−3592
−670
−559
−3345
−3691
−3092
−3380
−2887
−2074
−315
−1780
−1028
118



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9394


119(K
−2616
−3410
−2980
−1881
−4228
−3175
−1148
−3580
2804
−3243
−2552
1766
−3139
−724
2483
−2484
−2330
−3325
−3099
−2957
119



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9392


120(Q
−1950
−2899
−2068
−1380
−3575
−2688
−1085
−3122
5
−2965
−2212
−1472
−2788
3499
1706
−1878
1393
−2788
−2974
−2647
120



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9390


121(F)
−4720
−3927
−5139
−5454
4513
−4504
−2520
−3938
−5326
−3307
−3429
−4469
−4782
−4575
−4818
−4787
−4810
−4172
−1812
−736
121



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9388


122(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
122



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9386


123(F)
−4150
−3318
−4992
−5163
3777
−4801
−1232
−2695
−4739
954
−2105
−3556
−4637
−3592
−4183
−4031
−4023
−3011
−484
2534
123



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9384


124(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
124



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9382


125(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
125



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9379


126(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
126



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9377


127(I)
−2878
−2414
−5081
−4778
2015
−4697
−2956
3488
−4533
−580
−695
−4245
−4462
−3882
−4297
−4026
−2840
−329
−2190
−1350
127



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9375


128(P)
−1112
−1752
−3015
−3242
−4210
2077
−3186
−4033
−3529
−4251
−3331
−2415
3138
−3114
−3525
1101
−1561
−2858
−4391
−4208
128



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9373


129(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
129



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9371


130(H
−4836
−4275
−4356
−4629
−3663
−4242
5422
−5932
−4492
−5436
−5298
−4579
−4696
−4620
−4352
−5005
−5030
−5648
−3750
−3259
130



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9369


131(M
726
2516
−3320
−2699
1540
−2560
−1464
−135
−2311
−587
3158
−2207
−2612
−1942
−2131
−1653
−952
1415
−1331
−989
131



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9366


132(A
2437
−1736
−2547
−2496
−3668
−1942
−2557
−3365
−2590
−3601
−2745
1872
−2625
−2364
−2796
−1269
2286
−2528
−3861
−3523
132



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9364


133(P)
2199
−1920
−3558
−3810
−4266
−2163
−3478
−4045
−3859
−4314
−3481
−2754
3455
−3500
−3727
−1576
−1785
−2985
−4394
−4324
133



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9362


134(E
−2302
−4193
1609
3106
−4369
−2263
−1582
−4283
−1777
−4150
−3462
−851
−2781
1870
−2594
−1969
−2359
−3762
−4338
−3341
134



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9360


135(T
−1509
−1748
−4032
−3964
−2803
−2637
−3374
−581
−3680
−2093
−1801
−3053
−3237
−3460
−3596
−1989
3160
2274
−3655
−3308
135



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9358


136(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
136



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9355


137(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
137



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9353


138(S)
1402
−1678
−3661
−3893
−4185
−1940
−3408
−3979
−3851
−4254
−3335
−2598
−2733
−3421
−3678
3267
−1525
−2799
−4407
−4277
138



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9351


139(I)
−2917
−2413
−5393
−4971
−1597
−5119
−4331
3518
−4766
1140
−403
−4828
−4654
−4126
−4574
−4483
−2863
−105
−3287
−3176
139



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9349


140(H
−2691
−3511
−1351
−1619
−2597
−2858
4977
−4322
−2002
−4099
−3606
1623
−3363
−2027
−2266
−2576
−2835
−3872
−2991
−2056
140



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9347


141(E
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
141



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9344


142(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
142



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9342


143(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
143



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9340


144(E
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
144



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9338


145(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4358
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
145



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9335


146(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
146



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9333


147(Y
−4791
−3935
−4914
−5231
−815
−4530
−2178
−4357
−5024
−3745
−3807
−4206
−4772
−4328
−4584
−4677
−4837
−4435
−1477
4857
147



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9331


148(C
2078
2882
−3724
−3518
−2695
−2001
−2704
−2106
−3218
−2589
−1876
−2467
−2661
−2861
−3107
1882
−1336
1253
−3116
−2838
148



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9329


149(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4358
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
149



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9326


150(S)
1113
−1682
−3645
−3884
−4190
−1942
−3410
−3986
−3853
−4261
−3343
−2598
−2736
−3423
−3680
3340
−1530
−2804
−4411
−4280
150



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9324


151(H
−2323
−2561
−3097
−3086
−1647
−3098
4888
−2143
−2537
−2630
−2333
−2801
−3547
−2728
−2559
−2581
−2516
1445
−2255
−1333
151



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9322


152(A
2928
−1667
−3720
−3939
−4168
−1934
−3412
−3952
−3863
−4230
−3311
−2605
−2728
−3429
−3681
2146
−1516
−2782
−4394
−4269
152



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9320


153(Y
−3656
−3073
−4655
−4705
2072
−4415
−1160
−2834
−4311
−2480
−2370
−3332
−4386
−3385
−3897
−3630
1330
−2897
−449
4200
153



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9317


154(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
154



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9315


155(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
155



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9313


156(I)
1599
−1799
−4782
−4398
−2369
−4319
−3996
2490
−4241
−1351
−1186
−4067
−4263
−4075
−4278
−3599
−2201
2395
−3673
−3229
156



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9311


157(F)
−3470
−2920
−5747
−5177
3140
−5423
−3794
−632
−4917
2061
2648
−5046
−4645
−3788
−4437
−4711
−3319
−1432
−2615
−2450
157



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9308


158(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
158



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9306


159(N
−2152
−3226
−1659
−1246
−3822
−2724
−1126
−3401
−63
−3176
−2432
3327
−2852
1866
1828
−2010
−2006
−3064
−3127
−2771
159



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9304


160(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
160



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9301


161(D
−2185
−4023
2971
1468
−4227
−2230
−1509
−4119
−1656
−4000
−3274
1608
−2722
−1160
−2448
972
−2231
−3604
−4191
−3227
161



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9299


162(L)
−3047
−2911
−3749
−3527
−1475
−4117
−2954
−1124
−2671
2926
−473
−3500
−4084
1801
−2645
−3601
−3002
−1759
−2782
−2505
162



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9297


163(I)
−2388
−1937
−4948
−4533
1635
−4599
−3887
3268
−4365
−905
−875
−4243
−4379
−4047
−4325
−3869
−2360
1592
−3319
−2869
163



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9295


164(V
1092
−1556
−3968
−3774
−2577
−2468
−3041
−599
−3503
−2045
−1609
−2850
−3046
−3196
−3407
−1780
1216
3071
−3285
−2961
164



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9292


165(F)
1547
−939
−2765
−2207
1594
−2286
−1398
−514
−1927
−906
−256
−1896
1121
−1657
−1937
−1398
1348
1100
−1513
−1155
165



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9290


166(C
1272
5052
−4189
−4423
−4093
1091
−3532
−3812
−4117
−4134
−3233
−2716
−2738
−3626
−3808
−1304
−1508
−2711
−4345
−4265
166



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9288


167(V
−2483
−1992
−5115
−4742
−2252
−4880
−4534
1808
−4643
723
−1005
−4536
−4599
−4405
−4684
−4208
−2462
3131
−3865
−3512
167



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9285


168(V
699
−1835
−4861
−4496
−2442
−4434
−4193
1822
−4360
−1374
−1231
−4183
−4356
−4220
−4421
−3732
−2257
3136
−3831
−3371
168



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9283


169(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
169



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9281


170(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
170



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9278


171(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
171



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9276


172(E
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
172



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9274


173(A
3289
−1671
−3729
−3971
−4173
−1939
−3433
−3954
−3901
−4239
−3324
−2617
−2734
−3460
−3704
1119
−1523
−2786
−4402
−4279
173



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9271


174(E
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
174



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9269


175(T
−2430
−2823
−4179
−4462
−4591
−3027
−4037
−4454
−4406
−4707
−4191
−3664
−3724
−4237
−4215
−2689
4010
−3747
−4431
−4588
175



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9267


176(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
176



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9264


177(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
177



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9262


178(L)
−2381
−2370
−4412
−4172
−1521
−3608
−3357
−845
−3741
2871
−437
−3695
−3832
−3365
−3575
−2971
1469
−1264
−2889
−2744
178



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9260


179(A
3191
−1777
−3712
−3682
−2796
−2273
−3050
−2040
−3349
−2216
2634
−2731
−2945
−3125
−3255
−1630
−1688
−1822
−3467
−3179
179



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9257


180(T
1105
−1691
−3751
−3950
−4036
−1980
−3391
−3579
−3788
−4004
−3179
−2639
−2764
−3418
−3611
−1351
3649
−2631
−4299
−4165
180



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9255


181(S)
1115
−1654
−3622
−3791
−4192
1188
−3347
−3991
−3762
−4236
−3295
−2550
−2702
−3327
−3634
3032
−1489
−2786
−4402
−4277
181



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9253


182(
−5598
−4432
−5385
−5722
−3380
−4524
−4140
−5941
−5643
−5318
−5354
−5427
−4938
−5474
−5178
−5909
−5751
−5890
6275
−2993
182



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9250


183(H
−2817
−3584
−1743
−1758
−2728
−3054
4625
−4009
−1063
−3728
−3189
−1926
−3378
2752
−1170
−2680
−2782
−3708
−2912
−2121
183



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9248


184(S)
2113
−1671
−3691
−3909
−4175
−1935
−3404
−3963
−3845
−4238
−3318
−2599
−2728
−3415
−3673
2974
−1518
−2788
−4398
−4270
184



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9246


185(N
−1667
−2295
−1303
−1206
−1708
−2454
2688
1228
−1127
−2276
−1659
3307
−2730
−1191
−1399
−1726
−1677
−1853
−2144
−1341
185



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9243


186(K
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
186



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9241


187(F)
−3793
−3163
−5295
−5191
4063
−5003
−2160
−1329
−4845
1075
−704
−4205
−4663
−3781
−4356
−4376
−3689
−1985
−1339
−323
187



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9238


188(I)
−2691
−2201
−5254
−4813
−1747
−4966
−4275
2399
−4658
2193
−527
−4632
−4563
−4118
−4528
−4270
−2643
1553
−3385
−3255
188



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9236


189(N
−2608
−4312
1902
−698
−4665
−2423
−1924
−4776
−2319
−4628
−4060
3855
−3013
−1633
−3242
−2275
−2738
−4189
−4662
−3683
189



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9234


190(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
190



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9231


191(A
1597
−1385
−1621
−1030
−1453
−2189
−892
994
1158
−1265
−571
−1153
−2261
−699
1188
−1186
−908
900
−1781
−1350
191



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9229


192(R
−1138
−2535
−738
1346
−2893
−1985
−675
−2612
−163
−2551
−1669
1495
−2128
−235
2008
−1012
1851
−2189
−2703
−2067
192



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9226


193(D
−2243
−3522
3665
−856
−4525
−2330
−2015
−4560
−2391
−4501
−3857
−1194
−2947
−1733
−3224
1260
−2461
−3845
−4571
−3690
193



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9224


194(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
194



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9222


195(A
3150
−1781
−3954
−3897
−2697
−2590
−3290
1498
−3639
−1978
−1734
−3003
−3197
−3386
−3549
−1945
−1810
−689
−3544
−3207
195



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9219


196(V
−2448
−1959
−5109
−4794
−2563
−4877
−4851
1886
−4730
−1341
−1284
−4579
−4666
−4653
−4865
−4248
−2447
3388
−4252
−3753
196



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9217


197(L)
−3405
−2846
−5779
−5255
−1228
−5518
−4333
1719
−5019
2902
−18
−5245
−4745
−3976
−4603
−4888
−3285
−936
−3002
−3110
197



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9214


198(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
198



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9212


199(I)
−2459
−1963
−5126
−4808
−2568
−4914
−4889
3247
−4751
−1338
−1283
−4603
−4685
−4676
−4891
−4286
−2455
2426
−4274
−3777
199



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9209


200(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4358
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
200



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9207


201(H
−2395
−4306
1759
1612
−4428
−2292
4419
−4404
−1894
−4265
−3611
−881
−2831
−1320
−2741
−2046
−2464
−3880
−4428
−3405
201



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9205


202(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4358
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
202



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9202


203(N
−3642
−3838
−3004
−3356
−4743
−3612
−3773
−5643
−4099
−5460
−5060
4378
−4185
−3865
−4225
−3744
−3950
−5002
−4391
−4381
203



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9200


204(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
204



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9197


205(Y
−4606
−3570
−5022
−5359
2254
−4894
−1139
−3493
−4923
−2816
−2906
−3546
−4765
−3679
−4305
−4161
−4469
−3657
−387
4510
205



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9195


206(K
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
206



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9192


207(I)
−3202
−2787
−5004
−4996
−2836
−4384
−4440
3937
−4838
−1815
−1871
−4704
−4600
−4683
−4704
−4353
−3280
−603
−3938
−3661
207



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9190


208(S)
1943
−1773
−2482
−2503
−3907
−1935
−2647
−3686
−2717
−3870
−2975
1555
−2640
−2445
−2934
2632
−1475
−2699
−4064
−3728
208



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9187


209(N
−2546
−4182
−358
1608
−4553
−2427
−1858
−4587
−2065
−4451
−3846
3842
−2989
−1553
−2799
−2233
−2653
−4040
−4492
−3586
209



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9185


210(P)
−3075
−3411
−3652
−3377
−4713
−3421
−2871
−4754
−1885
−4551
−3970
−3201
3874
−2637
1884
−3201
−3246
−4233
−4063
−4155
210



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9182


211(T
−1093
−1706
−3487
−3693
−4074
−1966
−3302
−3842
−3638
−4134
−3253
−2558
−2748
−3296
−3520
1110
3646
−2760
−4304
−4125
211



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9180


212(I)
−2500
−2007
−5132
−4756
−2220
−4898
−4538
2959
−4657
869
−973
−4554
−4605
−4399
−4689
−4227
−2477
2250
−3844
−3510
212



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9177


213(Y
−3774
−3094
−5016
−5007
2147
−4760
−1471
−1948
−4610
2513
−1319
−3670
−4532
−3545
−4108
−3980
−3652
−2427
−717
2621
213



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9175


214(A
2561
−1647
−3667
−3813
−4167
1400
−3339
−3961
−3748
−4207
−3269
−2554
−2698
−3319
−3621
1972
−1482
−2770
−4380
−4254
214



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9172


215(R
−2761
3372
−4411
−3701
−4318
−3291
−2762
−4186
−1690
−4160
−3592
−3256
−3742
−2538
3812
−2949
−2961
−3734
−3870
−3866
215



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9170


216(I)
−1976
−1690
−4334
−3831
−1599
−3763
−2958
3100
−3513
−670
2664
−3459
−3733
−3184
−3413
−2951
1215
192
−2661
−2363
216



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9167


217(S)
−1740
−2857
1787
−890
−4083
−2148
−1822
−3944
−1950
−3947
−3166
−1164
2282
−1503
−2599
2462
−1955
−3236
−4123
−3368
217



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9165


218(D
−1796
−3465
2956
1278
−3721
−2140
2354
−3541
1157
−3446
−2631
−742
−2513
−824
−1706
−1558
−1793
−3064
−3617
−2792
218



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9162


219(D
−1468
−2962
2158
2010
−3256
−2035
−1014
−3010
−825
−2993
−2150
−673
−2334
−612
−1413
1100
−1455
908
−3204
−2451
219



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9160


220(E
−2636
−4572
1783
3438
−4760
−2391
−1842
−4773
−2236
−4607
−4040
−996
−2973
−1538
−3199
−2254
−2736
−4219
−4732
−3692
220



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9157


221(L)
−3421
−2860
−5790
−5264
−1219
−5529
−4333
1589
−5025
2937
−9
−5258
−4748
−3973
−4603
−4902
−3300
−965
−2995
−3106
221



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9155


222(H
−1434
−2670
−1420
1033
−3099
−2316
2338
−2728
1395
511
−1776
−966
−2367
−301
2245
−1323
−1317
−2360
−2690
−2208
222



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9152


223(K
1469
−2443
1279
−267
−2862
−1879
−707
−2612
1960
−2574
−1677
−575
−2071
−271
−856
1040
−1018
−2154
−2755
−2069
223



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9150


224(F)
−3694
−3088
−5579
−5263
3299
−5296
−2867
−919
−4969
2350
−235
−4680
−4707
−3837
−4468
−4661
−3559
−1683
−1918
−1128
224



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9147


225(F)
−4720
−3927
−5139
−5454
4513
−4504
−2520
−3938
−5326
−3307
−3429
−4469
−4782
−4575
−4818
−4787
−4810
−4172
−1812
−736
225



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9145


226(E
688
−2344
−716
2314
−2761
−1878
−641
−2492
−210
−2459
−1564
−580
−2036
−201
1256
1020
−948
−2051
−2637
−1982
226



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9142


227(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
227



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9140


228(Y
−1597
−1396
−3573
−3051
2007
−3040
−1283
−840
−2686
801
2421
1263
−3058
−2245
−2516
−2133
−1532
−823
−882
2863
228



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9137


229(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
229



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9134


230(Y
−4620
−3593
−5015
−5337
280
−4863
−1187
−3570
−4864
−2899
−2980
−3577
−4762
−3702
−4279
−4188
−4490
−3719
3436
4551
230



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9132


231(H
−862
−2311
−632
1878
−2629
−1803
2148
−2371
1173
−2322
−1409
−442
−1911
−24
−537
854
933
−1933
−2493
−1820
231



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9129


232(P)
−1820
−2216
−3792
−3938
−3452
−2669
−3505
−2107
−3787
−3057
−2729
−3136
3804
−3614
−3691
−2143
−2196
1506
−3938
−3681
232



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9127


233(Y
996
−1294
−2366
−1763
−954
−2474
−1131
−841
−1179
533
−479
−1680
−2552
−1243
1281
−1538
−1120
−749
−1438
3229
233



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9124


234(F)
−1969
−1770
−3543
966
3139
−3305
−1223
1499
−2815
−1359
−912
−2576
−3319
−2375
−2708
−2407
−1903
−1180
−760
2316
234



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9122


235(V
−2342
−1893
−4914
−4505
1622
−4568
−3913
1826
−4342
−1030
−963
−4213
−4369
−4072
−4326
−3838
−2317
3076
−3397
−2930
235



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9119


236(E
722
−2323
−653
2387
−2684
−1948
−848
−2315
−559
−2434
−1603
1662
−2171
−449
−1065
−1048
−1111
813
−2716
−2073
236



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8986
−894
−1115
−701
−1378
−9818
−9116


237(G
917
−3198
1792
−603
−3972
2355
−1545
−3811
−1605
−3767
−2983
1737
−2642
−1194
−2291
−1663
−1944
−3223
−3965
−3136
237



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1266
−7944
−790
−894
−1115
−701
−1378
−9818
−9114


238(D
−1223
−2437
2763
82
−3208
2150
−912
−3128
−1082
−3109
−2410
−254
−1932
−608
−1769
−1060
−1365
−2572
−3146
−2456
238



−149
−500
233
43
−376
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−649
−1491
−7744
−157
−3277
−2587
−263
−9818
−9111


239(D
−2102
−2902
3872
−571
−3494
−1911
−1556
−3716
−1893
−3662
−3195
−920
−2449
−1361
−2516
−1915
−2242
−3285
−3201
−2890
240



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−23
−6702
−7744
−894
−1115
−2587
−263
−9818
−9109


240(N
−694
−999
−1141
−940
−1064
−1767
−919
2282
−849
−787
−349
2352
−2038
−817
−1093
−1000
−743
143
−1624
−1073
241



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−23
−6702
−7744
−894
−1115
−562
−1632
−9818
−9106


241(H
−1431
−2871
−230
2243
−3163
−1925
3560
−3017
−719
−2971
−2155
−593
−2261
−564
−1242
1041
−1433
−2572
−3114
−2362
242



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7670
−8713
−894
−1115
−1555
−600
−9818
−9103


242(D
−2381
−4329
3618
1594
−4505
−2138
−1588
−4512
−1982
−4352
−3784
−740
−2721
−1283
−2949
−1999
−2481
−3960
−4490
−3437
243



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7670
−8713
−894
−1115
−345
−2232
−9818
−9101


243(P)
−1293
−1824
−1648
−1248
−1123
−2332
2376
−1662
−1068
−1836
−1165
−1350
2605
−1031
−1373
−1420
1364
−1468
−1606
2015
244



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9098


244(M
683
−1426
−1284
1643
−1482
−2033
−782
−1054
−631
424
2181
−936
1207
−541
−1010
−1010
−810
−892
−1806
−1337
245



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9095


245(S)
861
−1834
1340
−516
−2191
−1877
−758
−1838
−462
203
−1169
−752
−2056
−387
−921
1571
1006
−1523
−2319
−1756
246



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9093


246(M
−1263
−1161
−3038
−2448
−745
−2739
−1453
1314
−2054
−532
3663
−2170
−2762
1690
−2026
−1815
−1199
−464
−1382
1937
247



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9090


247(H
−2671
−3516
−1308
−1575
−2624
−2837
4896
−4310
−1982
−4092
−3590
1952
−3342
−1994
−2257
−2551
−2812
−3859
−3012
−2078
248



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9087


248(R
−2804
−3490
−3516
−2098
−4381
−3320
−1175
−3662
2379
−3289
−2620
−1979
−3242
2620
2657
−2671
−2465
−3437
−3117
−3033
249



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9085


249(R
732
−1128
−2794
−2187
−1101
−2570
−1459
−356
−1683
1417
−262
−1988
−2651
−1613
1995
−1657
−1104
1378
−1647
−1297
250



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9082


250(M
923
−1665
−3899
−3427
1737
−3128
−2177
−660
−3081
−421
4324
−2915
−3268
−2649
−2926
−2316
−1774
−849
−1851
−1321
251



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9079


251(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
252



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9077


252(E
1863
−2261
−1000
2576
−3283
−2007
−1447
−2956
−1252
−3072
−2244
−1127
−2442
−1087
−1727
−1275
1155
−2422
−3329
−2724
253



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9074


253(T
−1822
−1611
−4151
−3676
−1688
−3491
−2844
186
−3366
815
−670
−3264
−3577
−3075
−3294
−2693
2637
2187
−2655
−2324
254



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9071


254(M
−3494
−2937
−5788
−5208
2737
−5472
−3898
−624
−4954
2288
2851
−5116
−4665
−3807
−4466
−4768
−3340
−1435
−2676
−2586
255



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9069


255(D
−2671
−4634
3718
1448
−4812
−2395
−1863
−4839
−2290
−4667
−4121
−999
−2987
−1563
−3285
−2280
−2777
−4278
−4793
−3731
256



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9066


256(
−807
−1865
984
−295
−2038
−1850
2023
698
1219
−1817
−996
−567
−1940
−139
−647
−780
1016
−1409
2991
−1556
257



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9063


257(C
2301
2673
−4116
−3625
−1761
−3338
−2704
1666
−3330
−1177
−798
−3141
−3451
−3036
−3235
−2529
−1663
1516
−2564
−2191
258



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9061


258(F)
−1716
−1417
−4136
−3595
2444
−3534
−2588
1707
−3287
−928
−598
−3178
−3504
−2965
−3167
−2682
1032
2265
−2368
−1993
259



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9058


259(E
−2313
−4187
2435
2792
−4402
−2263
−1610
−4323
−1844
−4194
−3514
−860
−2792
−1276
−2693
882
−2382
−3790
−4388
−3377
260



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9055


260(E
−1939
−3595
1643
2581
−3872
−2212
−1304
−3690
−1146
−3580
−2792
−818
−2607
−925
2340
−1689
−1941
−3216
−3731
−2921
261



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9053


261(I)
−3202
−2787
−5004
−4996
−2836
−4384
−4440
3937
−4838
−1815
−1871
−4704
−4600
−4683
−4704
−4353
−3280
−603
−3938
−3661
262



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9050


262(H
−1017
2728
−2493
−1992
−1270
−2240
3922
−987
−1659
529
−639
−1791
−2500
−1540
−1774
784
−1071
−839
−1737
−1261
263



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9047


263(Q
741
−3277
2434
−357
−3577
−2095
−1155
−3386
−1029
−3313
−2481
1522
−2449
2598
−1654
−1458
−1671
−2910
−3496
−2688
264



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9044


264(I)
−3202
−2787
−5004
−4996
−2836
−4384
−4440
3937
−4838
−1815
−1871
−4704
−4600
−4683
−4704
−4353
−3280
−603
−3938
−3661
265



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9042


265(Q
−2715
−3506
−2454
−1838
−4264
−3140
−1326
−3747
2319
−3422
−2758
−1861
−3215
3713
−73
−2577
−2482
−3480
−3261
−3082
266



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7944
−8987
−894
−1115
−701
−1378
−9818
−9039


266(H
766
−2665
−1250
−679
−3083
−2257
3314
−2738
2115
−2633
−1790
−913
−2340
1628
−214
−1286
−1299
−2357
−2720
−2207
267



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7945
−8987
−894
−1115
−701
−1378
−9818
−9036


267(A
1483
−2544
1329
1148
−2853
−1866
−632
−2614
−278
−2555
−1651
1353
−2033
−189
1407
−900
−994
−2162
−2727
−2019
268



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7945
−8987
−894
−1115
−701
−1378
−9818
−9033


268(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
269



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7945
−8987
−894
−1115
−701
−1378
−9818
−9031


269(R
−2965
−3547
−3408
−2282
−4379
−3379
−1388
−3839
12
−3467
−2835
−2157
−3384
1874
3734
−2857
−2671
−3611
−3250
−3161
270



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1308
−7945
−761
−894
−1115
−701
−1378
−9818
−9028


270(N
−1039
−2562
586
2367
−2858
−1300
−470
−2699
−385
−2652
−1895
2515
−1724
−111
−973
−813
−1067
−2247
−2807
−2007
271



−149
−500
235
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−714
−1382
−7703
−151
−3331
−2609
−258
−9818
−9025


271(N
−622
−1477
−200
−357
−2707
2061
−999
−2606
−952
−2696
−1925
2838
−1767
−722
−1374
−664
−868
−1957
−2760
−2210
273



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−24
−6661
−7703
−894
−1115
−156
−3286
−9818
−9022


272(S)
−828
−2001
−805
1032
−2258
−1836
−533
−1931
−149
−2007
−1158
−547
−1941
−119
1234
1440
1026
880
−2282
−1677
274



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−9020


273(N
−1190
−2573
1355
−283
−2861
654
−809
−2615
−530
−2616
−1754
2375
−2165
−395
−1071
−1062
1012
−2205
−2829
1781
275



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−9017


274(Q
645
−2328
1160
−107
−2646
495
−476
−2397
−72
−2344
−1425
1283
−1901
1657
1361
−730
−798
−1950
−2514
−1827
276



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−9014


275(M
−816
−2072
1026
1269
−2307
−1806
−491
649
−121
−2050
1968
1387
−1908
−67
−614
−740
927
−1644
−2309
−1678
277



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−9011


276(C
897
2636
−1616
1312
−2623
1770
−1334
−2281
−1102
−2451
−1631
−1244
−2278
−1004
−1507
−1024
1250
−1839
−2782
−2281
278



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−242
−7945
−2754
−894
−1115
−701
−1378
−9818
−9008


277(S)
−911
−1183
−1682
1126
−1300
−2191
−1054
1565
−1062
−1045
−424
−1278
−2298
−937
−1360
1632
−874
1229
−1746
−1317
279



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7716
−8758
−894
−1115
−374
−2130
−9818
−9006


278(R
−2689
−3297
−3482
−2098
−4001
−3278
−1187
1210
1825
−3091
−2443
−1979
−3214
−777
3313
−2610
−2385
−3099
−3035
−2916
280



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−9003


279(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
281



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−9000


280(R
778
−2256
−849
1049
1188
−1910
−526
−2252
1217
−2239
−1363
−569
−1997
−87
2088
−847
−872
−1870
−2431
−1819
282



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8997


281(
−4593
−3601
−4995
−5296
213
−4817
−1247
−3585
−4805
−2925
−3000
−3610
−4749
−3723
−4255
−4202
−4477
−3731
5803
2567
283



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8994


282(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
284



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8992


283(M
−2922
−2503
−5243
−4802
−1395
−4799
−3922
1630
−4451
−67
4721
−4580
−4460
−3821
−4212
−4136
−2877
−524
−2992
−2887
285



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8989


284(I)
−2495
−2001
−5129
−4756
−2240
−4897
−4552
2929
−4659
812
−991
−4553
−4608
−4412
−4697
−4228
−2473
2322
−3865
−3522
286



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8986


285(I)
−2456
−1959
−5125
−4808
−2579
−4919
−4900
3028
−4754
−1349
−1292
−4605
−4688
−4684
−4898
−4291
−2452
2714
−4288
−3786
287



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8983


286(M
848
−1916
−4474
−3859
1726
−3859
−2665
−466
−3512
2254
2464
−3496
−3662
−2906
−3258
−2991
−2116
−895
−2138
−1962
288



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8980


287(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
289



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8977


288(T
−1068
−1511
−3017
−2766
−2440
−2076
−2321
−1926
−2515
691
−1630
−2213
−2655
−2340
−2580
984
3190
−1612
−2890
−2538
290



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8974


289(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
291



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8972


290(K
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
292



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8969


291(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
293



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8966


292(
−3761
−3778
−4018
−3442
−2596
−3886
−2358
−4281
1706
−3948
−3562
−3262
−4095
−2403
−1546
−3781
−3651
−4139
5825
−2093
294



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8963


293(T
−1328
−1922
−3470
−3733
−4307
2277
−3460
−4117
−3841
−4377
−3512
−2726
−2930
−3472
−3723
−1571
3260
−3014
−4415
−4352
295



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8960


294(C
−1973
4291
−4548
−4851
−4702
3097
−4084
−4671
−4689
−4886
−4115
−3509
−3445
−4310
−4354
−2238
−2433
−3616
−4524
−4782
296



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8957


295(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
297



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8954


296(K
−2049
−3206
1214
−1125
−3777
−2660
−1057
−3338
3118
−3117
−2350
−1305
−2771
−635
1683
−1896
−1900
−2990
−3089
−2711
298



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8951


297(Y
−3980
−3319
−4251
999
2912
−4528
−1135
−3159
−4198
−2674
−2627
−3261
−4474
−3325
−3891
−3769
−3878
−3246
−434
3896
299



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1892
−7945
−465
−894
−1115
−701
−1378
−9818
−8948


298(N
−682
−1389
−43
−192
−1821
−1114
−672
−2026
−553
−2169
−1573
3502
−1613
−485
−863
−706
−866
−1616
−1943
−1374
300



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−2832
−218
−9818
−8945


299(D
−1183
−2113
3476
202
−2559
−1197
−709
−2583
−900
−2640
−2095
−138
−1700
−471
−1494
−1021
−1294
−2203
−2437
−1969
301



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−2832
−218
−9818
−8943


300(
−2006
−1640
−2485
−2452
132
−2122
−859
−1689
−1966
−1425
−1294
−2093
−2439
−1935
−1852
−2230
−2075
−1731
5734
501
302



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−2832
−218
−9818
−8940


301(S)
87
−526
−885
−918
−1797
−699
−997
−1679
−970
−1948
−1267
−663
−1335
−874
−1147
2826
−252
−1082
−2036
−1577
303



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−2832
−218
−9818
−8937


302(G
−822
−1159
−1384
−1565
−2531
3361
−1634
−2586
−1803
−2721
−2161
−1396
−1832
−1670
−1866
−1023
−1160
−1997
−2255
−2352
304



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−2832
−218
−9818
−8934


303(P)
−1003
−1278
−1425
−1532
−2259
−1370
−1531
−2247
−1572
−2349
−1911
−1434
3795
−1555
−1648
−1199
−1287
−1861
−2108
−2096
305



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−2832
−218
−9818
−8931


304(K
−1075
−1727
−849
−488
−2222
−1560
−298
−1928
3141
−1906
−1299
−589
−1788
39
576
−1050
−1018
−1673
−1848
−1572
306



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−133
−3510
−9818
−8928


305(V
−2351
−1948
−4836
−4325
1729
−4359
−3437
1662
−4074
1144
−429
−4011
−4122
−3586
−3910
−3565
−2297
2576
−2861
−2632
307



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8925


306(D
−2623
−4424
3671
−647
−4716
−2401
−1896
−4807
−2315
−4650
−4093
1964
−2996
−1602
−3278
−2267
−2747
−4224
−4722
−3696
308



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8922


307(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
309



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1892
−7945
−465
−894
−1115
−701
−1378
−9818
−8919


308(K
−1075
−1727
−849
−488
−2222
−1560
−298
−1928
3141
−1906
−1299
−589
−1788
39
576
−1050
−1018
−1673
−1848
−1572
310



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−2832
−218
−9818
−8916


309(G
−822
−1159
−1384
−1565
−2531
3361
−1634
−2586
−1803
−2721
−2161
−1396
−1832
−1670
−1866
−1023
−1160
−1997
−2255
−2352
311



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−35
−6088
−7130
−894
−1115
−133
−3510
−9818
−8913


310(M
−972
−1126
−2062
−1461
−1092
−2307
2087
−624
1939
525
2294
−1457
−2364
−1036
−1244
−1332
−906
985
−1529
−1141
312



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8910


311(K
−1820
−2479
−2019
−1558
1878
−2659
−1288
−2471
2648
−2477
−1862
−1639
2280
−1101
−838
−1862
−1798
−2256
−2259
−1515
313



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8907


312(T
−1151
−994
−3207
−2614
−1014
−2726
−1631
1486
−2292
872
−195
1131
−2765
−1990
−2222
−1813
1899
1286
−1587
−1232
314



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8904


313(E
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
315



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8901


314(G
−2817
−3261
−2719
−3002
−3965
3464
2847
−5005
−3386
−4897
−4328
−2988
−3770
−3306
−3516
−2944
−3148
−4263
−3982
−3566
316



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8898


315(F)
−1106
−1561
−3154
−2946
2579
−2093
−2290
−2229
−2773
−2503
−1842
−2289
−2698
−2501
−2812
2488
1579
−1836
−2597
−1956
317



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8895


316(
−4550
−3570
−5043
−5348
3560
−4834
−1253
−3407
−4915
−2729
−2820
−3625
−4750
−3739
−4322
−4199
−4435
−3608
4998
679
318



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8892


317(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
319



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8889


318(A
3015
−1667
−3723
−3946
−4169
−1935
−3415
−3952
−3869
−4231
−3312
−2607
−2729
−3434
−3685
1976
−1516
−2782
−4395
−4270
320



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8886


319(H
−4836
−4275
−4356
−4629
−3663
−4242
5422
−5932
−4492
−5436
−5298
−4579
−4696
−4620
−4352
−5005
−5030
−5648
−3750
−3259
321



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8883


320(Q
−4207
−4117
−3531
−3731
−4752
−3930
−3721
−5554
−3395
−5179
−4897
−3823
−4399
4556
−3405
−4301
−4380
−5208
−4301
−4384
322



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8880


321(V
−2838
−2616
−4834
−4880
−3213
−3955
−4416
−610
−4757
−2310
−2253
−4383
−4345
−4643
−4642
−3732
−3007
3763
−4157
−3886
323



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8877


322(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
324



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8873


323(L)
−3647
−3060
−5937
−5371
−1118
−5682
−4332
−610
−5087
2996
2562
−5429
−4784
−3924
−4592
−5073
−3493
−1434
−2915
−3081
325



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8870


324(A
1628
−2000
−1147
−671
−2849
708
−956
−2576
1413
−2602
−1707
−874
1246
−539
−1044
862
−977
−2054
−2809
−2207
326



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8867


325(G
−994
−1140
−2933
−2481
−1432
1651
−1741
−844
−2217
1593
−640
−2068
−2570
−1955
−2226
912
−1096
1103
−1926
−1575
327



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8864


326(M
1630
−1391
944
−805
−1486
−2021
−819
−1063
−696
342
2038
−972
−2133
−594
−1070
829
−812
−889
−1808
−1343
328



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8861


327(R
741
−2275
−1345
−792
−2855
−2120
2241
−2529
−225
−2528
−1693
−987
−2298
−476
2769
943
−1193
−2130
−2695
−2176
329



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8858


328(E
−921
−2389
1203
1772
−2695
−1817
−532
−2446
1276
−2398
−1488
−451
−1950
−84
−661
−792
1210
−2005
−2575
1454
330



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8855


329(N
−1665
−3235
1720
−369
−3518
−2098
2328
−3335
−1031
−3275
−2450
2949
−2451
−767
−1649
−1460
1173
−2871
−3460
−2660
331



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8852


330(M
−984
−1840
−1021
1102
−2105
−1976
−854
−1713
−582
−1892
2223
−850
1910
−518
−1007
−1020
1988
−1453
−2293
−1758
332



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8849


331(A
2196
−2790
−511
1218
−3438
973
−1226
−3215
−1068
−3198
−2356
1800
−2422
−839
−1642
−1356
−1536
−2702
−3401
−2670
333



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8845


332(H
−1254
−2578
−716
−424
−2897
−2040
3715
−2651
−343
−2622
−1770
1388
−2228
1875
−752
−1137
1164
−2249
−2782
−2139
334



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8842


333(L)
−2624
−2340
−4474
−3875
1811
−4126
−2401
−789
−3050
2542
−178
−3525
−3908
−2863
1521
−3305
−2528
−1300
−1886
−1260
335



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8839


334(N
−994
−2499
1168
1208
−2810
−1843
−590
−2571
1159
−2510
−1599
1604
1101
1558
−751
−854
−943
−2117
−2678
−1972
336



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8836


335(Q
−1074
−1608
−1378
1055
−1583
−2212
−948
−1034
−735
1334
−653
−1095
−2304
2285
−1076
−1227
−1014
962
−1974
−1519
337



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8833


336(L)
−3687
−3085
−5735
−5323
1918
−5462
−3361
−783
−5056
2956
−82
−4983
−4746
−3886
−4547
−4848
−3542
−1585
−2287
−1717
338



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8830


337(E
−1470
−2699
−964
2371
−3064
−2232
−901
−2597
2215
−2668
−1868
−905
−2396
−486
−559
−1359
−1400
909
−2856
−2297
339



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8827


338(E
690
−2696
1523
1869
−2999
734
−738
−2770
1285
−2707
−1812
−539
−2120
−303
−973
−1012
−1128
−2313
−2880
−2153
340



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8823


339(
−5598
−4432
−5385
−5722
−3380
−4524
−4140
−5941
−5643
−5318
−5354
−5427
−4938
−5474
−5178
−5909
−5751
−5890
6275
−2993
341



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8820


340(M
−3596
−3028
−5873
−5313
−1121
−5593
−4267
−607
−5011
2392
4057
−5338
−4750
−3897
−4538
−4973
−3450
−1415
−2899
−3039
342



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8817


341(K
−991
−2413
−818
1144
−2767
−1924
−530
−2487
1895
−2410
−1508
1562
−2015
−77
1221
−869
1088
−2056
−2547
−1918
343



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8814


342(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
344



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8811


343(Y
−4791
−3935
−4914
−5231
−815
−4530
−2178
−4357
−5024
−3745
−3807
−4206
−4772
−4328
−4584
−4677
−4837
−4435
−1477
4857
345



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8807


344(K
−2113
−3150
−2249
−1388
−3797
−2829
−992
−3281
2710
−3026
−2265
1725
−2831
1860
1625
−1985
−1911
−2964
−2962
−2675
346



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8804


345(P)
−2272
3432
−4625
−4892
−4644
−2928
−4139
−4609
−4669
−4819
−4180
−3725
3909
−4404
−4368
−2543
−2718
−3748
−4454
−4686
347



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8801


346(E
−2460
−4036
−460
3371
−4348
−2457
−1702
−4275
−1584
−4118
−3462
−1104
−2946
2051
−2090
−2167
−2503
−3804
−4163
−3385
348



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8798


347(E
−2181
−3963
1851
3002
−4213
−2236
−1529
−4092
−1674
−3990
−3269
−843
−2732
−1182
−2455
−1882
1292
−3581
−4185
−3235
349



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8794


348(L)
−3686
−3084
−5725
−5318
2066
−5451
−3323
−791
−5050
2922
−90
−4961
−4743
−3882
−4541
−4834
−3541
−1590
−2260
−1671
350



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8791


349(F)
−3772
−3147
−5332
−5198
4004
−5042
−2246
−1256
−4859
1259
−621
−4264
−4667
−3786
−4369
−4411
−3663
−1931
−1412
−419
351



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8788


350(D
−2611
−4416
3402
−640
−4705
−2395
−1885
−4789
−2297
−4633
−4071
2681
−2988
−1589
−3255
−2256
−2733
−4207
−4711
−3684
352



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8785


351(E
2436
−2868
−924
2744
−4080
−2309
−2015
−3815
−2053
−3929
−3209
−1413
−2875
−1718
−2576
−1853
−2106
−3206
−4090
−3471
353



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8781


352(H
692
−2743
2175
−223
−3038
−1936
2515
−2810
−480
−2751
−1863
1409
−2154
1594
−1031
−1057
−1179
−2357
−2927
−2196
354



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8778


353(G
−2929
−3168
−3518
−3782
−1975
3489
−2803
−4394
−4019
−4184
−3856
−3400
−3940
−3733
−3967
−3094
−3258
−3950
−2519
2220
355



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8775


354(S)
743
−1505
−1390
−843
−1747
−1981
−884
−1345
−674
588
−830
−999
−2148
−615
1093
1645
1025
−1123
−2016
−1540
356



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8771


355(P)
−2511
−2204
−4688
−4170
−1213
−4215
−3207
1516
−3824
2221
−98
−3895
2348
−3271
−3624
−3424
−2456
−677
−2586
−2462
357



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8768


356(K
−1302
−1414
−2419
−1840
−1577
−2693
−1530
1325
2435
−1189
−660
−1855
−2761
−1446
−1616
−1749
1313
1240
−2101
−1697
358



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8765


357(E
687
−2542
−623
1957
−2905
−1944
−710
−2641
1433
−2592
−1707
−604
1914
−274
−748
−998
−1082
−2205
−2761
−2094
359



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8762


358(E
−2040
−3771
1647
2163
−4056
1964
−1435
−3921
−1508
−3821
−3060
−809
−2658
−1075
−2248
864
−2081
−3410
−4011
−3098
360



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8758


359(I)
−2526
−2113
−4983
−4447
1598
−4503
−3494
2404
−4186
1749
−242
−4162
−4188
−3576
−3962
−3711
−2456
1420
−2790
−2649
361



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8755


360(R
−1519
−2765
−1433
1043
−3230
−2362
−786
−2853
1407
−2712
−1878
−1010
−2424
−346
2271
−1399
2090
−2477
−2766
−2295
362



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8752


361(A
2266
−3817
1712
2253
−4133
−2221
−1507
−3996
−1626
−3910
−3174
−845
−2706
−1158
−2386
−1834
−2166
−3485
−4107
−3184
363



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8748


362(F)
−1042
−885
−3196
−2582
2439
−2571
2436
1238
−2213
706
10
−2148
−2609
−1856
−2072
−1653
1024
−245
−1243
−832
364



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8745


363(M
2115
−1084
−3069
−2501
−1009
−2553
−1611
−411
−2186
950
2374
−2167
−2680
−1891
−2150
−1666
1057
−397
−1620
−1292
365



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8741


364(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
366



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8738


365(K
−1362
−2752
−891
1110
−3142
−2149
−769
−2837
2560
−2727
−1865
−782
1373
1618
−441
−1221
−1281
−2420
−2829
−2241
367



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8735


366(G
1022
−1909
−2732
−2555
−3858
2953
−2403
−3572
−1903
−3702
−2863
−2142
−2736
−2152
1711
−1431
−1592
−2728
−3841
−3572
368



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8731


367(E
−1926
−3649
1536
2753
−3890
−2165
−1327
−3735
−1325
−3640
−2851
1567
−2586
−953
−2024
−1665
1092
−3244
−3826
−2947
369



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8728


368(K
−2153
−2373
−3052
−2232
−1767
−3262
−1649
−1215
3030
859
2514
−2219
−3217
−1370
−831
−2425
−2024
−1487
−2493
−2241
370



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8725


369(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
371



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8721


370(M
−3269
−2747
−5652
−5079
−1177
−5325
−4102
1525
−4829
1255
4460
−5012
−4616
−3829
−4428
−4616
−3145
−974
−2895
−3004
372



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8718


371(G
2030
−1651
−3665
−3855
−4195
2704
−3377
−3993
−3822
−4242
−3302
−2568
−2705
−3371
−3670
1117
−1491
−2786
−4410
−4294
373



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8714


372(A
2364
−1861
−1953
−1763
−3466
−1952
−1976
−3199
−1760
−3331
−2471
−1666
−2522
1987
−2080
1936
−1374
−2468
−3555
−3102
374



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8711


373(N
−1618
−2562
−1583
−1250
−3475
−2356
−1296
−3134
−474
−3082
−2296
3378
−2657
−908
1726
−1605
1129
−2676
−3159
−2725
375



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8707


374(P)
−1913
−2274
−2279
−2086
−1517
−2783
2782
−2081
−1589
560
−1612
−2058
3317
−1785
−1716
−2097
−1976
−2000
−2063
−1260
376



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8704


375(H
−929
−2010
−791
1272
−2044
−1913
2963
−1853
−298
−1959
−1145
1363
−2029
−239
−766
−888
−872
799
−2186
1512
377



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8700


376(A
2877
−1669
−3800
−3976
−4022
−1962
−3382
−3596
−3795
−3999
−3156
−2631
−2746
−3409
−3616
−1328
2512
−2627
−4284
−4151
378



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8697


377(N
−3122
−3352
−2328
−2577
−596
−3446
−1619
−3701
−2981
−3309
−3133
3888
−3831
−2693
−3130
−3026
−3247
−3561
−1270
2585
379



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8693


378(G
−2018
−2692
−1922
−2271
−4544
3363
−2969
−4678
−3249
−4731
−3990
1984
−3232
−2825
−3564
−2140
−2400
−3690
−4460
−4184
380



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8690


379(G
−1257
−1874
−3207
−3523
−4341
3341
−3408
−4248
−3853
−4463
−3557
−2605
−2866
−3402
−3760
1410
−1718
−3031
−4475
−4358
381



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8687


380(Y
−1236
−1100
−3220
−2594
−757
−2737
−1473
−388
−2061
1865
−99
−2233
−2760
−1870
1132
−1823
−1172
1244
−1376
2076
382



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8683


381(L)
−2379
−2141
1254
−3407
−1292
−3975
−2923
1518
−3405
2543
−203
−3359
−3842
−2943
−3392
−3194
−2319
−538
−2579
−2378
383



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8679


382(R
−1224
−1996
−1644
−1058
−2408
−2207
−1005
−2022
−378
315
−1393
−1189
1416
−662
2668
937
−1215
−1747
−2459
−2006
384



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−288
−7945
−2519
−894
−1115
−701
−1378
−9818
−8676


383(R
−1347
−2594
−872
1389
−3097
−2078
−754
−2768
−37
−2671
−1846
−784
−2259
−333
2876
1137
−1287
−2364
−2765
−2230
385



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7671
−8713
−894
−1115
−1555
−600
−9818
−8672


384(N
−1422
−3052
1734
1461
−3326
980
−901
−3136
−759
−3056
−2213
1864
−2210
−500
−1385
1028
−1413
−2660
−3236
−2429
386



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7671
−8714
−894
−1115
−345
−2232
−9818
−8669


385(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4358
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
387



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8665


386(K
−1622
−2790
−1624
1030
−3260
−2461
−828
−2846
2708
−2716
−1905
−1116
−2506
−393
1496
−1511
−1487
892
−2768
−2339
388



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8662


387(M
−1888
−1623
−4198
−3600
−1092
−3572
−2482
−158
−3255
2140
2397
−3210
−3470
−2776
−3059
−2699
1034
1479
−2114
−1890
389



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8658


388(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
390



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8655


389(D
−2522
−4504
3422
1446
−4683
938
−1750
−4661
−2127
−4504
−3910
−909
−2894
−1438
−3092
−2147
−2619
−4102
−4695
−3603
391



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8651


390(
−3715
−3061
−5054
−5000
2784
−4779
−1574
−1778
−4602
1668
−1132
−3738
−4524
−3553
−4110
−4005
−3593
−2304
4571
289
392



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8647


391(H
−1137
−2529
1169
−405
−2911
−2042
2148
−2611
1514
−2512
−1626
−678
−2128
1722
2034
−1013
−1053
−2189
−2623
−2031
393



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8644


392(Q
−1523
−3117
2166
1365
−3403
−2057
−1004
−3194
1438
−3110
−2253
−667
−2351
2526
−1325
−1325
−1499
−2728
−3276
−2509
394



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−9818
−8640


393(Y
−4598
−3559
−5006
−5329
2257
−4898
2594
−3515
−4887
−2848
−2926
−3523
−4759
−3654
−4280
−4143
−4453
−3662
−366
4237
395



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−9818
−8637


394(A
2380
−2381
−1061
1282
−3846
2045
−1941
−3614
−1958
−3704
−2890
−1385
−2665
−1636
−2457
−1509
−1729
−2895
−3915
−3319
396



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−9818
−8633


395(V
−2097
−1741
−4406
−4026
−2222
−4103
−3555
2399
−3840
−1319
−1108
1444
−4071
−3681
−3882
−3349
−2094
2762
−3366
−2920
397



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−9818
−8629


396(D
−928
−2371
1886
−165
−2715
−1822
−563
−2464
1387
−2421
−1512
−478
1080
−116
−692
750
1043
−2018
−2599
−1913
398



*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*



*
*
*
*
*
*
*
−9818
0

















TABLE 8








HMMER2.0 [2.3.2]



NAME XFP



ACC PF03894.6



DESC D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase



LENG 187



ALPH Amino



RF no



CS no



MAP yes



COM hmmbuild -f -F HMM fs.ann SEED.ann



COM hmmcalibrate --seed 0 HMM fs.ann



NSEQ 6



DATE Sun Apr. 29 15:58:33 2007



CKSUM 8559



GA 15.5 15.5



TC 18.2 15.7



NC 13.6 14.2



XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4



NULT −4 −8455



NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644



EVD −10 339054 0.664450
































A
C
D
E
F
G
H
I
K














HMM
m -> m
m -> i
m -> d
i -> m
i -> i
d -> m
d -> d
b -> m
m -> e
L
M
N
P
Q
R
S
T
V
W
X
Y






−222
*
−2807




















 1(M)
−919
−1308
−2565
−2222
−1930
−1975
−1841
−1394
−1946
−1641
3218
−1876
1316
−1826
−2064
−1219
2730
−1183
−2403
−2047
1



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7717
−8760
−894
−1115
−701
−1378
−1222
−8539














 2(R)
691
−1720
−837
−294
−1975
−1756
−501
673
−144
−1751
−937
1196
−1878
−121
1881
951
−681
−1325
−2078
−1516
2



−149
−500
233
43
−381
399
106
−626
−210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7717
−8760
−894
−1115
−701
−1378
−8762
−8535














 3(V)
−885
−1621
−999
990
−1982
−1862
−915
−1514
−733
−1796
−1048
−873
1044
−622
−1147
858
−916
2279
−2227
−1717
3



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7717
−8760
−894
−1115
−701
−1378
−8762
−8531














 4(L)
−2842
−2379
−5209
−4634
−994
−4802
−3633
−174
−4364
2574
2538
−4478
−4252
−3491
−4019
−4045
−2735
1455
−2614
−2661
4



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7717
−8760
−894
−1115
−701
−1378
−8762
−8527














 5(G)
−1046
−1667
−2864
−3162
−4077
3372
−3114
−3979
−3511
−4193
−3299
−2337
−2643
−3089
−3461
1104
−1501
−2797
−4216
−4066
5



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7717
−8760
−894
−1115
−701
−1378
−8762
−8524














 6(K)
696
−1959
−661
−115
−2242
−1707
−387
−1932
1754
−1971
−1104
−400
−1809
1727
−429
726
−657
544
−2214
−1595
6



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7717
−8760
−894
−1115
−701
−1378
−8762
−8520














 7(Y)
−4363
−3343
−4802
−5127
2939
−4674
−927
−3251
−4692
−2586
−2669
−3329
−4545
−3458
−4081
−3935
−4227
−3413
−177
4223
7



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7717
−8760
−894
−1115
−701
−1378
−8762
−8516














 8(C)
−1627
2687
−3970
−3367
1521
−3283
−2148
−37
−3007
2149
118
−2930
−3201
−2520
−2790
−2405
−1562
1206
−1785
−1498
8



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8512














 9(R)
737
−2550
1594
−497
−3149
−1980
−968
−2870
−526
−2846
−2019
−774
−2281
−570
3052
−1221
−1340
−2417
−3004
−2383
9



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8508














 10(D)
−2399
−4358
3324
2308
−4527
−2166
−1608
−4527
−1994
−4367
−3790
−765
−2746
−1301
−2656
−2018
−2496
−3976
−4515
−3456
10



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8504














 11(V)
−2284
−1804
−4895
−4504
−1990
−4629
−4189
2412
−4382
1011
−761
−4283
−4358
−4111
−4394
−3938
−2259
2686
−3549
−3219
11



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8500














 12(M)
−2278
−1867
−4746
−4224
−1303
−4284
−3343
1656
−3973
1240
3304
−3939
−3999
−3423
−3784
−3494
−2217
2073
−2691
−2538
12



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8496














 13(K)
693
−2202
−1423
−749
−2553
−2182
−674
−2107
2480
−2170
−1388
−928
−2252
−275
1432
−1210
−1146
1012
−2378
−1932
13



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8492














 14(N)
−1049
−1434
−1773
−1500
−1636
−2109
−1397
−1127
−1366
1815
−824
2287
−2427
−1298
−1590
−1307
1405
−1004
−2112
−1674
14



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8488














 15(N)
−3232
−3514
−2565
−2905
−4358
−3267
−3364
−5192
−3627
−5059
−4632
4348
−3843
−3410
−3792
−3319
−3533
−4568
−4087
−3976
15



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−867
−7718
−1171
−894
−1115
−701
−1378
−8762
−8484














 16(E)
−1220
−2441
99
2757
−2966
−1568
−808
−2774
−675
−2772
−2036
−363
2075
−466
−1190
−1070
−1278
−2337
−2879
−2244
16



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−22
−6873
−7915
−894
−1115
−2169
−363
−8762
−8480














 17(D)
−1004
−1749
2891
−305
−2104
−1632
−964
−886
−981
−1762
−1266
−575
−2040
−692
−1495
−1057
−1089
1706
−2499
−1874
17



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−460
−6873
−1933
−894
−1115
−216
−2846
−8762
−8476














 18(M)
823
−1578
1383
−173
−1837
−1620
−411
−1480
−107
−1624
2041
−425
938
−38
−569
−595
1047
−1187
−1968
−1402
18



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7493
−8536
−894
−1115
−1355
−715
−8762
−8472














 19(T)
−897
−2121
−888
−291
−2541
−1825
−413
−2206
1465
−2164
−1310
−532
−1923
20
1428
1124
1894
−1823
−2313
−1770
19



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7493
−8536
−894
−1115
−404
−2032
−8762
−8468














 20(N)
−867
−2024
−881
−423
−2654
−1782
−693
−2361
−230
−2371
−1499
−2664
−2000
−273
1178
870
1294
−1909
−2569
−1974
20



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8464














 21(F)
−3465
−2876
−5057
−4893
3938
−4762
−2035
−1021
−4547
1305
−409
−4001
−4411
−3532
−4090
−4106
−3362
−1663
−1215
−228
21



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8459














 22(R)
−4070
−3868
−4406
−3890
−4820
−3837
−3026
−5122
−1946
−4739
−4314
−3731
−4155
−2844
4177
−4178
−4041
−4836
−3949
−4248
22



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8455














 23(I)
−2379
−1901
−4955
−4531
−1728
−4662
−4059
2900
−4381
1570
−512
−4321
−4336
−3965
−4313
−3959
−2342
1650
−3306
−3087
23



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8451














 24(F)
−2676
−2354
−4538
−4394
3992
−3956
−1878
−728
−4074
−869
−959
−3497
−4006
−3383
−3780
−3277
−2708
1235
−1189
−154
24



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8447














 25(G)
−1031
−1653
−2874
−3165
−4069
3287
−3106
−3964
−3500
−4180
−3282
−2329
−2632
−3078
−3451
1430
−1486
−2782
−4212
−4060
25



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8443














 26(P)
−4077
−3791
−4518
−4858
−5206
−3818
−4460
−5898
−5053
−5602
−5343
−4628
4289
−4938
−4749
−4364
−4429
−5313
−4385
−5119
26



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8439














 27(D)
−3832
−4189
4132
−2236
−5073
−3341
−3194
−5633
−3716
−5365
−5035
−2584
−3886
−3102
−4252
−3662
−4000
−5151
−4434
−4544
27



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8435














 28(E)
−3777
−4125
−1925
3871
−4984
−3355
−3125
−5379
−3371
−5156
−4796
−2611
−3875
−3007
−3762
−3630
−3916
−4960
−4357
−4463
28



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8430














 29(T)
−1009
−1466
−1761
−1492
−1840
−2028
−1429
−1343
−1350
766
−1045
1393
−2396
−1299
−1587
−1239
2910
−1154
−2256
−1801
29



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8426














 30(M)
1499
−1643
−917
1243
−1962
−1786
−691
−1586
−425
−1763
2853
−693
−1975
−349
−862
779
−773
−1304
−2139
−1601
30



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8422














 31(S)
−1644
−2173
−3321
−3615
−4077
−2357
−3411
−4307
−3792
−4459
−3725
−2859
−3095
−3512
−3692
3619
−2088
−3285
−4091
−3959
31



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8418














 32(N)
−3232
−3514
−2565
−2905
−4358
−3267
−3364
−5192
−3627
−5059
−4632
4348
−3843
−3410
−3792
−3319
−3533
−4568
−4087
−3976
32



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8414














 33(R)
−2793
−3369
−3662
−2108
−4312
−3237
−1071
−3580
2493
−3189
−2545
−1934
−3162
−654
3328
−2667
−2432
−3373
−2988
−2947
33



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−14
−7718
−8760
−894
−1115
−701
−1378
−8762
−8409














 34(L)
−3410
−2836
−5464
−5039
1728
−5176
−3154
−570
−4762
2953
115
−4708
−4504
−3649
−4284
−4539
−3272
−1347
−2103
−1550
34



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8406














 35(W)
−901
−2337
1407
−65
−2541
−1747
2100
−2368
−133
−2334
−1447
1385
−1904
1561
−644
−770
−849
−1946
3634
−1773
35



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8401














 36(A)
2155
−3109
1523
1273
−3479
1112
−1076
−3288
−1004
−3231
−2417
−592
−2329
−693
−1651
−1350
−1580
−2804
−3429
−2615
36



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−145
−7718
−8760
−894
−1115
−701
−1378
−8762
−8397














 37(V)
1023
−958
−3146
−2600
−1103
−2513
−1686
63
−2290
−801
2200
−2204
−2668
−2000
−2237
−1650
1119
2408
−1689
−1335
37



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8392














 38(F)
−3852
−3057
−4741
−4884
3335
−4537
−1011
−2489
−4461
957
−1921
−3313
−4388
−3349
−3926
−3759
−3730
−2762
−269
3290
38



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8388














 39(E)
−2083
−3395
−722
2943
−3878
−2383
−1214
−3575
2285
−3395
−2666
−1041
−2696
−830
−750
−1870
−2023
−3182
−3392
−2873
39



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8384














 40(V)
−1086
−1419
−2113
−1929
−1724
796
2671
−1274
−1766
−1761
−1123
−1760
−2533
−1690
−1904
−1385
−1234
2825
−2172
−1669
40



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8379














 41(T)
−869
−1487
−3137
−3257
−3760
−1746
−2945
−3506
−3200
−3791
−2920
−2268
−2512
−2898
−3156
−2152
3197
−2486
−3990
−3777
41



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8375














 42(K)
−1040
−2442
−581
1137
−2735
−1868
−548
−2490
2386
−2427
−1554
1311
−2012
−114
−440
−908
−975
−2076
−2562
1837
42



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8371














 43(R)
−2795
−3370
−3663
−2111
−4313
−3239
−1074
−3582
2384
−3191
−2548
−1936
−3164
−657
3395
−2670
−2434
−3375
−2990
−2949
43



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−291
−7718
−2514
−894
−1115
−701
−1378
−8762
−8366














 44(Q)
−1085
−1755
−1145
−887
1733
985
−888
−1755
−814
−1851
−1208
−1026
−2253
3209
−1142
−1159
−1127
−1521
−1673
−859
44



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7443
−8484
−894
−1115
−1459
−652
−8762
−8362














 45(W)
−4735
−3754
−4703
−4993
−2377
−3915
−3273
−4870
−4782
−4319
−4338
−4620
−4317
−4639
−4390
−4998
−4881
−4870
−6237
−1984
45



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7443
−8486
−894
−1115
−372
−2136
−8762
−8358














 46(M)
−914
−874
871
−1922
−783
−2384
−1220
1249
−1670
662
3001
−1756
−2427
−1420
−1731
−1438
1257
−98
−1343
−990
46



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8353














 47(M)
641
−2071
−521
1084
−2362
−1649
−321
−2091
82
−2076
2071
1285
−1749
1468
−420
684
−616
−1678
−2276
−1612
47



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8349














 48(Q)
−757
−2178
−529
1705
−2487
519
−368
−2219
1195
−2182
−1278
−340
−1806
1749
−411
−642
−696
453
−2362
−1699
48



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8344














 49(Y)
−1288
−1144
−3267
−2757
−710
−2784
−1561
2546
−2393
−763
−315
−2345
−2866
−2105
−2314
−1913
1335
−38
−1365
2756
49



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8340














 50(K)
−1352
−2460
1542
−473
−2590
−2065
−876
−2234
1932
1737
−1566
−761
−2285
−505
−815
−1266
−1304
−1990
−2658
−2071
50



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8335














 51(P)
−1299
−2774
−236
1949
−3178
−1870
−886
−2961
−678
−2912
−2058
1447
2218
−481
−1251
890
−1302
−2487
−3099
−2347
51



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−291
−7718
−2514
−894
−1115
−701
−1378
−8762
−8331














 52(P)
1084
−2190
−341
2185
−2933
−1688
−929
−2655
−732
−2706
−1884
−590
2271
−556
−1245
−978
−1128
−2169
−2938
−2278
52



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7443
−8486
−894
−1115
−1459
−652
−8762
−8326














 53(N)
−1418
−2809
2479
−106
−2949
−1799
−926
−2766
−883
578
−2068
2542
−2188
−576
−1502
−1234
−1435
−2399
−3048
−2264
53



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−283
−7443
−2563
−894
−1115
−1459
−652
−8762
−8322














 54(D)
−888
−2124
2400
66
−2357
−1565
−509
−1956
−285
−2113
−1336
−255
−1834
1955
−808
−781
−871
1155
−2428
−1753
54



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−19
−7180
−8222
−894
−1115
−1865
−463
−8762
−8317














 55(D)
−1805
−3680
2903
2554
−3868
−1689
−1066
−3813
−1341
−3684
−3041
−289
−2233
−743
−2210
−1458
−1880
−3289
−3847
−2846
55



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−1334
−7180
−753
−894
−1115
−1865
−463
−8762
−8313














 56(G)
−515
−887
−1038
−1187
−2183
3187
−1282
−2175
−1398
−2342
−1770
−1048
−1555
−1281
−1496
−708
−838
−1625
−1974
−1993
56



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−41
−5887
−6930
−894
−1115
−2652
−250
−8762
−8308














 57(G)
−515
−887
−1038
−1187
−2183
3187
−1282
−2175
−1398
−2342
−1770
−1048
−1555
−1281
−1496
−708
−838
−1625
−1974
−1993
57



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−41
−5887
−6930
−894
−1115
−151
−3329
−8762
−8304














 58(E)
−1346
−1562
−1816
2436
2337
−2525
−1279
−603
−1533
−1038
−631
−1602
−2663
−1361
−1800
−1658
−1319
1113
−1614
−909
58



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8299














 59(H)
−933
−961
−2242
−1707
−764
−2320
2094
1132
−1393
1839
−65
1195
−2382
−1248
−1495
−1381
−874
−319
−1292
−847
59



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8295














 60(M)
−1293
−1342
−2996
−2500
−1038
−2617
−1796
−510
−2162
−890
−3410
−2240
−2790
−1938
−2186
2108
−1323
−630
−1840
−1502
60



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8290














 61(P)
2028
−1663
−1726
−1624
−3398
998
−1915
−3145
−1828
−3283
−2409
1487
2266
−1625
−2185
−1038
−1207
−2349
−3511
−3060
61



−149
−500
−233
43
−381
399
106
−626
−210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8285














 62(D)
−752
−2203
1771
1070
−2520
−1688
−359
−2261
1383
−2210
−1298
−324
−1796
94
1124
−630
−691
543
−2379
−1707
62



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−231
−7718
−2838
−894
−1115
−701
−1378
−8762
−8281














 63(R)
−796
−2105
−516
1238
−2496
1498
−421
−2206
49
−2188
−1314
−382
−1834
11
1567
−698
1155
−1794
−2375
−1748
63



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7504
−8546
−894
−1115
−412
−2011
−8762
−8276














 64(G)
−2419
−2885
−3067
−2886
−4406
3143
−2559
−4353
−1679
−4234
−3572
−2708
−3407
−2316
2444
−2556
2650
−3716
−3837
−3878
64



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8271














 65(R)
−1920
−2516
−2433
−1527
−2802
−2746
−961
1879
176
−2261
−1647
−1532
−2759
1746
2935
−1945
−1744
−2067
−2537
−2247
65



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8267














 66(V)
−2252
−1773
−4891
−4567
−2335
−4633
−4538
1812
−4482
−1132
−1073
−4339
−4440
−4385
−4597
−3992
−2250
3374
−3973
−3489
66



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8262














 67(M)
−849
−923
1670
−1529
−893
−2248
−1067
−233
−1327
600
2147
−1485
−2308
−1135
−1498
−1282
1243
1140
−1394
−1015
67



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8257














 68(E)
−1776
−3015
−373
3097
−3872
−2071
−1546
−3755
−1570
−3731
−2992
−934
−2595
−1219
−2164
1764
−1926
−3163
−3855
−3091
68



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8253














 69(M)
−1335
−1165
−3272
−2688
−1002
−2941
−1834
1451
−2360
−376
3121
−2427
−2930
2286
−2331
−2034
−1279
1529
−1766
−1445
69



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8248














 70(L)
−3777
−3243
−5089
−5032
−1735
−4514
−4040
−1299
−4754
−3261
696
−4891
−4508
−4152
−4395
−4705
−3767
−1993
−3125
−3069
70



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8243














 71(S)
−1644
−2173
−3321
−3615
−4077
−2357
−3411
−4307
−3792
−4459
−3725
−2859
−3095
−3512
−3692
3619
−2088
−3285
−4091
−3959
71



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8238














 72(E)
−3777
−4125
−1925
3871
−4984
−3355
−3125
−5379
−3371
−5156
−4796
−2611
−3875
−3007
−3762
−3630
−3916
−4960
−4357
−4463
72



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8234














 73(H)
−4406
−3961
−3921
−4171
−3231
−3926
5401
−5468
−3992
−5013
−4849
−4130
−4378
−4151
−3893
−4548
−4592
−5195
−3383
−2818
73



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8229














 74(Q)
−1172
−1561
−1803
−1343
−1477
−2292
−1190
−991
−908
1463
−565
−1415
−2454
2378
−1119
−1403
1904
−979
−1998
−1592
74



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8760
−894
−1115
−701
−1378
−8762
−8224














 75(C)
943
4854
−3669
−3391
−1886
−2164
−2449
−922
−3000
676
−1025
−2492
−2705
−2686
−2861
−1458
−1303
−805
−2559
−2242
75



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8761
−894
−1115
−701
−1378
−8762
−8219














 76(E)
−2096
−3637
−282
2979
−3947
−2215
−1377
−3824
−1173
−3688
−2987
−859
−2659
2792
−1646
−1832
−2117
−3370
−3761
−3011
76



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8761
−894
−1115
−701
−1378
−8762
−8214














 77(G)
−3681
−3593
−4389
−4747
−5281
3812
−4419
−5908
−5071
−5671
−5288
−4417
−4223
−4850
−4766
−3952
−4069
−5125
−4424
−5214
77



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8761
−894
−1115
−701
−1378
−8762
−8209














 78(W)
−4254
−3318
−4804
−5081
3098
−4588
−1050
−3111
−4645
−2453
−2534
−3403
−4509
−3506
−4073
−3950
−4146
−3314
5352
877
78



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8761
−894
−1115
−701
−1378
−8762
−8205














 79(L)
−3375
−2814
−5645
−5079
−928
−5372
−4042
−409
−4777
2894
2856
−5098
−4540
−3683
−4317
−4734
−3229
−1208
−2698
−2837
79



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8761
−894
−1115
−701
−1378
−8762
−8200














 80(E)
−2150
−3708
−263
3290
−4011
−2224
−1421
−3906
−1260
−3766
−3081
−868
−2687
2032
−1753
−1875
−2180
−3447
−3837
−3070
80



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8761
−894
−1115
−701
−1378
−8762
−8195














 81(G)
2022
−1618
−3291
−3557
−4069
3053
−3239
−3867
−3682
−4133
−3245
−2462
−2643
−3254
−3542
−1260
−1474
−2727
−4214
−4140
81



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7718
−8761
−894
−1115
−701
−1378
−8762
−8190














 82(Y)
−4444
−3646
−4593
−4892
−509
−4268
−1873
−3993
−4663
−3407
−3460
−3884
−4503
−3998
−4255
−4336
−4491
−4072
−1177
4829
82



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8185














 83(L)
−1931
−1973
−3870
−3595
−1374
−3144
−2842
−646
−3171
2519
−338
−3150
−3415
−2899
−3072
−2469
2215
−950
−2583
−2364
83



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8180














 84(L)
−3777
−3243
−5089
−5032
−1735
−4514
−4040
−1299
−4754
3261
−696
−4891
−4508
−4152
−4395
−4705
−3767
−1993
−3125
−3069
84



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8175














 85(T)
−2081
−2497
−3792
−4048
−4206
−2712
−3671
−3972
−3974
−4274
−3765
−3291
−3408
−3829
−3824
−2338
3976
−3321
−4129
−4203
85



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8170














 86(G)
−3681
−3593
−4389
−4747
−5281
3812
−4419
−5908
−5071
−5671
−5288
−4417
−4223
−4850
−4766
−3952
−4069
−5125
−4424
−5214
86



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8165














 87(R)
−4070
−3868
−4406
−3890
−4820
−3837
−3026
−5122
−1946
−4739
−4314
−3731
−4155
−2844
4177
−4178
−4041
−4836
−3949
−4248
87



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8160














 88(H)
−1602
−2202
−2033
−2051
−2524
−2310
4500
−3130
−1754
−3221
−2593
−1952
−2860
−1941
−1889
−1744
2351
−2641
−2857
−2142
88



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8155














 89(G)
1424
−1648
−3257
−3538
−4097
3299
−3253
−3902
−3697
−4166
−3283
−2476
−2666
−3269
−3560
−1290
−1506
−2760
−4228
−4161
89



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8150














 90(F)
653
−1307
−3869
−3274
2383
−3222
−2101
2307
−2923
979
54
−2851
−3158
−2477
−2735
−2341
−1496
−62
−1785
−1478
90



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8145














 91(F)
−4268
−3318
−4807
−5092
4134
−4606
−1026
−3118
−4656
−2457
−2538
−3389
−4515
−3495
−4075
−3946
−4154
−3320
3520
904
91



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8140














 92(A)
2436
−1450
−3080
−3062
−3722
−1707
−2801
−3478
−3005
−3713
−2813
−2174
1760
−2697
−3044
2014
−1242
−2447
−3930
−3709
92



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8134














 93(S)
−797
2971
−3664
−3672
−3546
−1712
−2998
−3220
−3388
−3539
−2685
−2356
−2477
−3023
−3254
−2975
−1394
−2305
−3830
−3649
93



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8139














 94(Y)
−4444
−3646
−4593
−4892
−509
−4268
−1873
−3993
−4663
−3407
−3460
−3884
−4503
−3998
−4255
−4336
−4491
−4072
−1177
4829
94



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8124














 95(E)
−3777
−4125
−1925
3871
−4984
−3355
−3125
−5379
−3371
−5156
−4796
−2611
−3875
−3007
−3762
−3930
−3916
−4960
−4357
−4463
95



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8119














 96(A)
2588
−1457
−3368
−3530
−3881
−1722
−3095
−3647
−3472
−3926
−3021
−2338
−2505
−3081
−3355
2536
−1295
−2531
−4108
−3950
96



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8114














 97(F)
−4350
−3618
−4843
−5137
−4483
−4245
−2206
−3493
−4990
−2887
−3007
−4147
−4512
−4243
−4506
−4433
−4439
−3737
−1500
−417
97



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8109














 98(M)
706
−1201
−3821
−3231
−1007
−3165
−2103
1510
−2884
1469
2600
−2801
−3121
−2481
−2711
−2285
−1405
1421
−1846
−1550
98



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−15
−7719
−8761
−894
−1115
−701
−1378
−8762
−8103














 99(H)
−1732
−2580
−1961
−1265
−3039
−2507
3869
−2740
117
−2656
−1919
−1338
−2617
−548
2281
−1696
1373
−2439
−2662
−2280
99



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8098














100(I)
−2262
−1772
−4916
−4590
−2360
−4692
−4608
2948
−4520
−1146
−1086
−4377
−4472
−4432
−4647
−4052
−2257
2744
−4024
−3536
100



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8093














101(V)
−2259
−1770
−4911
−4585
−2361
−4682
−4599
2472
−4513
−1151
−1088
−4370
−4467
−4427
−4640
−4043
−2254
3105
−4021
−3531
101



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8087














102(D)
−1281
−2033
3087
−908
−2698
−2028
−1475
−1596
−1454
−2381
−1758
−1118
−2459
−1191
−1938
−1365
1158
1286
−3025
−2452
102



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8082














103(S)
−1644
−2173
−3321
−3615
−4077
−2357
−3411
−4307
−3792
−4459
−3725
−2859
−3095
−3512
−3692
3619
−2088
−3285
−4091
−3959
103



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8077














104(M)
−3457
−3250
−4578
−4682
−2595
−3839
−3833
−2264
−4294
−1708
5248
−4331
−4225
−4184
−4072
−3938
−3655
−2653
−3435
−3236
104



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8071














105(L)
−2433
−2031
−4839
−4270
1637
−4331
−3217
1476
−3985
2288
96
−3990
−3978
−3290
−3710
−3525
−2348
1287
−2465
−2367
105



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8066














106(N)
−952
−1482
−1959
−1737
−2360
−1930
−1748
−1480
−1701
−2148
−1486
−2935
−2420
−1580
−1960
−1182
2024
1339
−2731
−2304
106



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8061














107(Q)
−3758
−3792
−3072
−3245
−4342
−3586
−3274
−5053
−2880
−4719
−4410
−3349
−4049
4526
−2916
−3829
−3917
−4726
−3973
−3953
107



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8055














108(H)
−3941
−3303
−3939
−4079
336
−4266
4844
−3317
−3580
−2730
−2734
−3097
−4295
−3109
−3362
−3663
−3868
−3403
−373
2757
108



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8050














109(A)
2381
−1319
−2661
−2353
1521
924
−2061
−2159
−2216
−2443
−1684
−1875
−2365
−1993
−2375
1314
−1085
−1685
−2841
−2472
109



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8044














110(K)
−3709
−3782
−3592
−3179
−4734
−3639
−2561
−4748
3938
−4426
−3926
−3130
−3916
−2292
−1410
−3723
−3624
−4465
−3818
−3998
110



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8039














111(W)
−4239
−3322
−4800
−5070
2397
−4565
−1086
−3101
−4634
−2445
−2527
−3425
−4503
−3523
−4074
−3959
−4140
−3310
5693
837
111



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8033














112(L)
−3174
−2633
−5520
−4994
−1029
−5242
−4053
1453
−4737
2932
174
−4959
−4514
−3738
−4340
−4596
−3057
−761
−2778
−2863
112



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8028














113(K)
−1073
−2547
1983
1205
−2885
−1851
−580
−2624
−2040
−2541
−1656
−477
−2021
−141
1183
−924
−1014
−2183
−2682
−2014
113



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8022














114(M)
1488
−784
−2685
−2089
−758
−2378
−1239
1284
−1794
−540
2454
−1834
−2427
1221
−1791
−1444
−817
1168
−1289
−933
114



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8017














115(C)
1116
3924
−3654
−3615
−3360
1383
−2916
−3025
−3341
−3335
−2502
−2336
−2465
−2960
−3214
−1051
2263
−2200
−3668
−3472
115



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−1842
−7719
−489
−894
−1115
−701
−1378
−8762
−8011














116(V)
−588
−579
−2160
−1946
−759
−1834
−1555
1143
−1670
−9
123
−1684
−2156
−1608
−1741
−1222
−711
2811
−1682
−1223
116



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−42
−5919
−6961
−894
−1115
−152
−3320
−8762
−8006














117(R)
740
−1533
−1479
−915
−1874
−1953
−873
−1460
−464
601
−933
−1028
−2151
−588
2555
747
−902
−1223
−2081
−1631
117



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−8000














118(E)
−1519
−3167
1458
2619
−3424
−1929
2323
−3240
−838
−3155
−2323
−534
−2279
1614
−1462
−1295
−1511
−2766
−3331
−2516
118



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7994














119(I)
−1108
−1022
−2831
−2246
−840
−2607
−1460
2037
−1908
1407
36
−2034
−2633
1279
−1920
−1682
1280
−126
−1499
−1169
119



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7989














120(P)
−1226
−2486
−584
−427
−3101
1037
−903
−2834
−1189
−2796
−1949
1466
2619
−497
−899
−1126
−1246
−2362
−2960
−2322
120



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7983














121(W)
−5218
−4132
−5085
−5404
−2925
−4252
−3753
−5470
−5268
−4877
−4907
−5071
−4661
−5108
−4833
−5506
−5369
−5443
−6261
−2535
121



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7977














122(R)
−2786
−3324
−3159
−2158
−3444
−3203
2835
−3646
19
−3307
−2690
−2048
−3245
−942
3648
−2697
−2534
−3415
−2888
−2551
122



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7972














123(H)
661
−2311
−894
−302
−2686
811
2065
−2371
1951
−2300
−1425
−565
−1991
−22
1309
−873
−899
−1971
−2431
−1856
123



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7966














124(D)
−1783
−3362
2774
−330
−3711
−2050
−1197
−3534
1468
−3445
−2669
−689
2225
−827
−1652
−1548
−1805
−3056
−3598
−2798
124



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7960














125(I)
−2784
−2321
−4408
−4214
2256
−4011
1193
3098
−3823
−1290
−1179
−3131
−3919
−3037
−3465
−3182
−2704
−1420
−513
2319
125



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8761
−894
−1115
−701
−1378
−8762
−7954














126(S)
1918
−1460
−2882
−2748
−3664
1367
−2596
−3426
−2688
−3630
−2719
−2048
1372
−2426
−2827
1999
−1206
−2419
−3840
−3573
126



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8762
−894
−1115
−701
−1378
−8762
−7948














127(S)
−1644
−2173
−3321
−3615
−4077
−2357
−3411
−4307
−3792
−4459
−3725
−2859
−3095
−3512
−3692
3619
−2088
−3285
−4091
−3959
127



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8762
−894
−1115
−701
−1378
−8762
−7943














128(I)
−2843
−2399
−5187
−4720
−1139
−4765
−3832
−83
4437
−2876
−38
−4542
−4330
−3660
−4145
−4094
−2781
1324
−2793
−2769
128



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8762
−894
−1115
−701
−1378
−8762
−7937














129(N)
−3232
−3514
−2565
−2905
−4358
−3267
−3364
−5192
−3627
−5059
−4632
4348
−3843
−3410
−3792
−3319
−3533
−4568
−4087
−3976
129



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8762
−894
−1115
−701
−1378
−8762
−7931














130(Y)
−1616
−1344
−3951
−3366
−912
−3313
−2164
1486
−3018
1578
−20
−2936
−3238
−2574
−2829
−2438
−1554
1298
−1825
2867
130



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7719
−8762
−894
−1115
−701
−1378
−8762
−7925














131(I)
−2438
−1963
−4986
−4538
−1573
−4670
−3957
−2309
−4367
2112
−365
−4331
−4311
−3863
−4246
−3955
−2391
1749
−3152
−2992
131



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7919














132(M)
694
−667
−2636
−2033
−642
−2235
−1106
−145
−1725
645
2178
−1733
−2301
−1435
−1691
561
1254
1172
−1134
−779
132



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7913














133(T)
−866
−1487
−3132
−3246
−3768
−1744
−2940
−3519
−3190
−3799
−2925
−2263
−2509
−2888
−3152
2652
2778
−2491
−3995
−3782
133



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7907














134(S)
−1644
−2173
−3321
−3615
−4077
−2357
−3411
−4307
−3792
−4459
−3725
−2859
−3095
−3512
−3692
3619
−2088
−3285
−4091
−3959
134



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7901














135(H)
−1254
−2216
−843
−755
−2687
−1970
3314
−2636
−839
−2710
−1932
1437
−2343
−837
−1206
−1244
2636
−2214
−2810
−2158
135



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7895














136(W)
−2217
−1998
4211
−3930
−1050
−3517
−2291
−446
−3456
−1314
−1150
−3322
−3697
−3202
−3309
−2906
−2265
3288
3612
−961
136



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7889














137(W)
1105
−2160
−3956
−3730
1959
−3434
−1130
−1795
−3348
−1692
−1411
−2810
−3571
−2764
−3122
−2642
−2381
−1804
5259
566
137



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7883














138(R)
−2269
−3123
−2497
−1540
−3728
−2880
2414
−3259
347
−2971
−2265
−1547
−2868
2539
3022
−2145
−2019
−2993
−2838
−2604
138



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7877














139(Q)
−3758
−3792
−3072
−3245
−4342
−3586
−3274
−5053
−2880
−4719
−4410
−3349
−4049
4526
−2916
−3829
−3917
−4726
−3973
−3953
139



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7870














140(D)
−2399
−4357
3309
2333
−4526
−2166
−1607
−4526
−1993
−4365
−3789
−765
−2745
−1300
−2954
−2017
−2495
−3974
−4514
−3455
140



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7864














141(H)
−4406
−3961
−3921
−4171
−3231
−3926
5401
−5468
−3992
−5013
−4849
−4130
−4378
−4151
−3893
−4548
−4592
−5195
−3383
−2818
141



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7858














142(N)
−3232
−3514
−2565
−2905
−4358
−3267
−3364
−5192
−3627
−5059
−4632
4348
−3843
−3410
−3792
−3319
−3533
−4568
−4087
−3976
142



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−16
−7720
−8762
−894
−1115
−701
−1378
−8762
−7852














143(G)
−3681
−3593
−4389
−4747
−5281
3812
−4419
−5908
−5071
−5671
−5288
−4417
−4223
−4850
−4766
−3952
−4069
−5125
−4424
−5214
143



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7845














144(F)
−4352
−3337
−4799
−5121
3888
−4670
−928
−3235
−4686
−2571
−2654
−3328
−4541
−3456
−4078
−3931
−4217
−3400
−178
−3260
144



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7839














145(T)
−866
−1487
−3132
−3246
−3768
−1744
−2940
−3519
−3190
−3799
−2625
−2263
−2509
−2888
−3152
2652
2778
−2491
−3995
−3782
145



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7833














146(H)
−4406
−3961
−3921
−4171
−3231
−3926
5401
−5468
−3992
−5013
−4849
−4130
−4378
−4151
−3893
−4548
−4592
−5195
−3383
−2818
146



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7827














147(Q)
−3758
−3792
−3072
−3245
−4342
−3586
−3274
−5053
−2880
−4719
−4410
−3349
−4049
4526
−2916
−3829
−3917
−4726
−3973
−3953
147



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7820














148(D)
−2001
−3292
3601
−626
−4238
−2111
−1759
−4246
−2092
−4199
−3539
−963
−2715
−1470
−2900
1255
−2206
−3561
−4288
−3412
148



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7814














149(P)
−4077
−3791
−4518
−4858
−5206
−3818
−4460
−5898
−5053
−5602
−5343
−4628
4289
−4938
−4749
−4364
−4429
−5313
−4385
−5119
149



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7807














150(G)
−2415
−2868
−3074
−2972
−4434
3359
−2704
−4428
−1915
−4330
−3670
−2780
−3430
−2492
−1875
−2564
−2676
−3754
−3904
−3964
150



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7801














151(F)
−2339
−1986
−4665
−4064
3176
−4095
−2859
−229
−3739
1224
2452
−3737
−3789
−3040
−3446
−3256
−2245
1216
−2175
−2011
151



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7794














152(I)
−2056
−1731
−4386
−3851
−1203
−3873
−2916
−2392
−3540
2065
−105
−3529
−3714
−3075
−3381
−3054
1098
51
−2437
−2237
152



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7788














153(D)
−1118
−2211
2195
−621
−3243
803
−1223
−3009
−1065
−3036
−2177
−857
−2246
−848
−1597
985
2152
−2413
−3246
−2588
153



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7781














154(H)
−1003
−1199
−2356
−1954
−1388
−2206
3132
−636
−1619
−1264
−667
−1770
−2496
−1555
−1734
−1385
1395
2407
−1879
−1430
154



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7775














155(M)
695
−1534
−4131
−3535
−944
−3528
−2430
2154
−3199
1674
2687
−3161
−3396
−2699
−2998
−2660
−1749
−182
−2022
−1814
155



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7768














156(L)
682
−1592
−4101
−3606
−1242
−3407
−2684
95
−3291
2495
−186
−3196
−3443
−2879
−3157
−2606
−1799
1329
−2351
−2114
156



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7762














157(N)
−1782
−3518
1546
1312
−3758
−1994
−1175
−3610
−1194
−3512
−2730
2977
−2426
−804
−1905
1148
−1804
−3115
−3698
−2808
157



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7755














158(K)
−2459
−2938
−2880
−2055
−2893
−3094
−1378
−2442
3407
840
−1882
−2005
−3159
−1028
−200
−2490
−2293
−2474
−2837
−2553
158



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7748














159(K)
−1148
−2117
−1407
−950
−3066
−1999
−1021
−2720
2980
−2713
−1884
−1076
−2995
−623
−548
−886
1179
−2234
−2851
−2379
159



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7741














160(P)
1034
−1295
−2486
−2150
1616
−1809
−1863
−1877
−2017
−2165
−1435
−1781
2897
−1813
−2195
966
−1055
−1493
−2567
−2152
160



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8762
−894
−1115
−701
−1378
−8762
−7735














161(D)
−2104
−3998
2881
1352
−4180
1216
−1393
−4095
−1610
−3965
−3275
1701
−2586
−1055
−2452
−1771
−2164
−3570
−4160
−3154
161



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−1843
−7720
−490
−894
−1115
−701
−1378
−8762
−7728














162(N)
−498
−1221
110
−15
−1620
−964
−477
−1773
−335
−1934
−1330
3292
−1450
−280
−647
−522
−670
−1383
−1763
−1178
162



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−43
−5920
−6962
−894
−1115
−2643
−252
−8762
−7721














163(D)
−961
−1901
3312
369
−2315
−1032
−511
−2292
−663
−2377
−1817
34
−1523
−264
−1242
−811
−1065
−1927
−2232
−1739
163



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−43
−5920
−6962
−894
−1115
−2643
−252
−8762
−7714














164(H)
−997
−1393
−635
−583
−386
−1411
4407
−1664
−259
−1594
−1144
−676
−1756
−466
−415
−1034
−1055
−1464
−790
42
164



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−43
−5920
−6962
−894
−1115
−152
−3320
−8762
−7707














165(V)
−2121
−1684
−4679
−4254
1449
−4310
−3633
2372
−4076
−839
−756
−3954
−4126
−3800
−4049
−3566
−2094
2791
−3155
−2717
165



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7720
−8763
−894
−1115
−701
−1378
−8762
−7700














166(V)
−1172
−1063
−3285
−2755
−1280
−2703
−1906
1464
−2465
−907
−407
−2383
−2845
−2193
−2434
949
1115
2471
−1913
−1547
166



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7693














167(R)
−2428
−3127
−2310
−1828
−3921
−2880
−1372
−3657
−155
−3392
−2732
1602
−3079
−997
3685
−2367
−2329
−3316
−3168
−2942
167



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7687














168(I)
673
−1102
−3159
−2611
−1247
−2870
−1866
2195
−2325
−820
−354
−2364
−2914
1287
−2345
−1977
−1221
2181
−1906
−1534
168



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7679














169(Y)
−4444
−3646
−4593
−4892
−509
−4268
−1873
−3993
−4663
−3407
−3460
−3884
−4503
−3998
−4255
−4336
−4491
−4072
−1177
4829
169



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7672














170(F)
−3400
−2827
−5361
−4990
2846
−5067
−2816
−651
−4697
2592
25
−4506
−4471
−3608
−4224
−4409
−3268
−1403
−1851
−1138
170



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7665














171(P)
1191
−1755
−3163
−3402
−3998
−1985
−3182
−3756
−3492
−4031
−3233
−2502
3818
−3181
−3412
−1407
−1613
−2766
−4124
−4023
171



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7658














172(P)
1902
−1437
−3147
−3131
−3577
−1724
−2798
−3245
−3019
−3540
−2684
−2204
3083
−2726
−3028
−1065
1432
−2336
−3823
−3602
172



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7651














173(D)
−3832
−4189
4132
−2236
−5073
−3341
−3194
−5633
−3716
−5365
−5035
−2584
−3886
−3102
−4252
−3662
−4000
−5151
−4434
−4544
173



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−17
−7721
−8763
−894
−1115
−701
−1378
−8762
−7644














174(A)
2913
−1375
−3689
−3354
−3146
1014
−2798
−2432
−3176
−3025
−2284
−2297
−2499
−2833
−3104
−1111
−1235
1120
−3514
−3272
174



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7722
−8764
−894
−1115
−701
−1378
−8762
−7638














175(N)
−3232
−3514
−2565
−2905
−4358
−3267
−3364
−5192
−3627
−5059
−4632
4348
−3843
−3410
−3792
−3319
−3533
−4568
−4087
−3976
175



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8764
−894
−1115
−701
−1378
−8762
−7629














176(C)
−775
3036
−2850
−2408
−1588
−1919
−1731
−1110
−2133
−1469
2376
−1909
−2363
−1881
−2164
925
2536
−890
−2035
−1692
176



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7622














177(L)
−3406
−2832
−5440
−5026
2063
−5151
−3069
−587
−4746
2880
97
−4659
−4495
−3638
−4269
−4506
−3269
−1359
−2041
−1444
177



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7615














178(L)
−3777
−3243
−5089
−5032
−1735
−4514
−4040
−1299
−4754
3261
−696
−4891
−4508
−4152
−4395
−4705
−3767
−1993
−3125
−3069
178



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7607














179(C)
2942
2982
−3827
−3914
−3641
−1706
−3124
−3330
−3613
−3649
−2779
−2414
−2485
−3185
−3403
1168
−1242
−2356
−3925
−3779
179



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7600














180(V)
−2004
−1608
−4544
−4142
−2135
−4125
−3700
1840
−3967
−1140
−967
−3830
−4052
−3798
−4000
−3390
1338
2999
−3399
−2963
180



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7592














181(F)
1588
−850
−2996
−2447
2261
−2299
−1475
−182
−2129
−756
−166
−2019
−2485
−1821
−2060
−1435
1085
1429
−1479
−1117
181



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7585














182(D)
1088
−3814
3226
1427
−4086
−2058
−1384
−3970
−1570
−3872
−3169
−670
−2570
−1045
−2378
−1733
−2102
−3453
−4076
−3107
182



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7577














183(H)
−2540
−3259
−3155
−1838
−4078
−3083
3373
−3420
2334
−3069
−2392
−1745
−3017
−555
2597
−2412
−2222
−3187
−2907
−2791
183



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7570














184(C)
998
4910
−3920
−3803
−2550
−2066
−2898
−917
−3459
−2125
−1635
−2602
−2717
−3092
−3274
−1392
−1333
1279
−3175
−2885
184



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7562














185(M)
−3195
−2670
−5480
−4897
2597
−5129
−3599
−426
−4630
2310
2844
−4767
−4399
−3550
−4175
−4399
−3050
−1206
−2449
−2343
185



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7555














186(Q)
−1978
−3010
−1873
−1184
−3573
−2640
2315
−3132
2388
−2890
−2142
−1292
−2679
3064
193
−1845
−1787
−2819
−2820
−2501
186



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249




−18
−7721
−8763
−894
−1115
−701
−1378
−8762
−7547














187(S)
−1150
−2122
1356
−858
−3376
−1832
−1484
−3135
−1404
−3212
−2374
−1042
−2342
−1143
−1916
2458
2054
−2488
−3434
−2812
187



*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*



*
*
*
*
*
*
*
−8762
0






























TABLE 9









HMMER2.0 [2.3.2]



NAME XFP C



ACC PF09363.1



DESC XFP C-terminal domain



LENG 212



ALPH Amino



RF no



CS no



MAP yes



COM hmmbuild -f -F HMM fs.ann SEED.ann



COM hmmcalibrate-seed 0 HMM fs.ann



NSEQ 6



DATE Thu May 3 17:57:02 2007



CKSUM 5491



GA 25.0 25.0



TC 47.0 29.4



NC 15.7 15.7



XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4



NULT −4 −8455



NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644



EVD −10.483098 0.640397

































A
C
D
E
F
G
H
I
K














HMM
m->m
m->i
m->d
i->m
i->i
d->m
d->d
b->m
m->e
L
M
N
P
Q
R
S
T
V
W
Y






−193
*
−2999


1(K)
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
1



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−1193
−8721


2(Q)
−1392
−2802
−618
−424
−3105
−2084
2223
−2893
−446
−2828
−1977
1616
1057
3114
−866
−1245
−1350
−2468
−2957
−2283
2



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8718


3(P)
1097
−2000
−3515
−3791
−4319
−2230
−3513
−4117
−3884
−4378
−3565
−2801
3889
−3540
−3757
−1661
−1871
−3066
−4416
−4363
3



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8714


4(H)
829
−2314
−845
−285
−2656
−1910
2103
−2366
0
−2324
−1434
−569
−2003
1714
2028
709
−889
−1956
−2490
−1870
4



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8711


5(W)
872
−1301
−1524
−969
−1324
−2103
−863
−924
−771
407
−486
−1096
1119
2314
−1087
−1098
−833
−778
2741
−1233
5



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8707


6(Q)
1139
−1910
−1850
−1613
−3267
−2003
−1790
−2923
−1462
−3080
−2266
−1578
−2518
3569
−1755
−1258
1437
−2329
−3351
−2895
6



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8704


7(W)
−4606
−3595
−5007
−5318
253
−4844
−1211
−3574
−4838
−2908
−2986
−3590
−4756
−3710
−4268
−4192
−4482
−3721
5473
3368
7



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8700


8(L)
−2343
−2040
−4542
−3937
1831
−4019
−2749
1548
−3592
2218
65
−3613
−3780
1482
−3353
−3156
−2252
−936
−2180
−1956
8



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8697


9(T)
−1385
−2322
1417
−1149
−3726
−2036
−1831
−3500
−1823
−3585
−2754
−1314
−2593
−1510
−2340
1921
2800
−2796
−3801
−3184
9



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8693


10(M)
−1554
−1399
−3470
−2849
−1043
−3109
−1939
−255
1071
1028
3713
−2584
−3080
−2156
−2304
−2204
−1488
1337
−1856
−1572
10



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8690


11(D)
−2255
−4080
2935
2308
−4312
−2253
−1576
−4208
−1768
−4097
−3398
−854
−2767
−1236
−2584
−1939
1141
−3689
−4293
−3313
11



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8687


12(E)
−1169
−2634
−621
2512
−2971
−1972
−696
−2711
1193
−2634
−1746
−602
−2134
1564
−675
777
−1111
−2271
−2782
−2114
12



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8683


13(A)
3176
−1703
−4006
−4070
−3246
−2260
−3401
−1441
−3823
−2722
−2304
−2855
−2973
−3487
−3663
−1620
−1659
1420
−3901
−3631
13



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8679


14(I)
−1158
−1549
−1727
1889
−1669
−2385
−1107
1890
−836
−1344
−736
−1323
−2456
−885
1317
−1399
−1097
1018
−2055
−1611
14



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8676


15(H)
848
−2322
−1179
−560
−2630
−2119
2363
−2275
2038
−2278
−1446
−801
−2188
−233
1325
−1089
−1073
627
−2469
−1939
15



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8672


16(H)
−1354
−2628
−576
1333
−2749
−2068
3770
−2560
−570
422
−1777
1432
−2292
−513
−1032
−1235
−1312
−2213
−2778
−2099
16



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8669


17(C)
947
4245
−3771
−3246
−1380
−2866
−2176
−26
−2899
843
−500
−2705
−3040
−2556
−2758
−2038
−1382
1326
−2062
−1723
17



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8665


18(T)
−1296
−2623
−1140
1900
−3034
−2174
−690
−2702
1486
−2593
−1730
−817
−2254
−245
1344
−1174
1932
−2302
−2686
−2143
18



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8662


19(A)
2122
−2640
1402
−254
−2963
−1940
−736
−2715
1187
−2660
−1774
−577
−2132
1562
−869
−1024
−1125
−2273
−2833
−2136
19



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8658


20(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
20



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8655


21(I)
1832
−1779
−4707
−4321
−2320
−4169
−3857
2857
−4145
−1329
−1156
−3958
−4165
−3960
−4165
−3443
−2157
1658
−3571
−3143
21



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8651


22(A)
1979
−2132
1358
−1230
−3624
895
−1764
−3399
−1659
−3464
−2589
−1343
−2501
−1420
−2138
1914
−1456
−2656
−3665
−3086
22



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8647


23(I)
−1613
−1432
−3370
991
−1684
−3301
−2327
2219
−2660
−1128
−723
−2715
−3318
−2464
−2756
−2418
1220
2185
−2433
−2038
23



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8644


24(W)
−3333
−2943
−4821
−4705
−865
−4258
−2319
1550
−4105
−1483
−1550
−3928
−4360
−3731
−3856
−3853
−3350
−1751
5765
−662
24



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8987
−894
−1115
−701
−1378
−8914
−8640


25(D)
−2155
−3941
2895
1331
−4165
−2244
−1467
−4031
2266
−3906
−3170
−838
−2712
−1110
−2170
−1857
−2186
−3532
−4076
−3168
25



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8637


26(W)
−4535
−3580
−5043
−5339
2264
−4800
−1315
−3404
−4909
−2727
−2820
−3667
−4744
−3774
−4331
−4221
−4434
−3613
5781
611
26



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8633


27(A)
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
27



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8629


28(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
28



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8626


29(N)
−1462
−2164
−1894
−2122
−3935
−2171
−2578
−3801
−2603
−4009
−3230
3172
−2856
−2392
−2846
−1616
2907
−2960
−4077
−3633
29



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8622


30(D)
2036
−3092
3356
−1144
−4437
−2344
−2201
−4300
−2537
−4380
−3702
−1420
−2974
−1935
−3248
−2004
−2335
−3577
−4473
−3774
30



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8618


31(K)
719
−2804
1286
−282
−3133
−1980
−846
−2900
2557
−2836
−1958
1613
−2216
−421
−1059
−1135
−1263
−2444
−3007
−2283
31



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8615


32(G)
−1795
−2851
1703
−1000
−4330
2898
−2022
−4220
−2294
−4229
−3471
−1259
−2791
−1732
−2991
1182
−2066
−3405
−4399
−3633
32



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1206
−7945
−837
−894
−1115
−701
−1378
−8914
−8611


33(N)
−713
−1555
−300
−476
−2841
2208
−1131
−2756
−1105
−2843
−2074
2836
−1863
−861
−1525
−760
−971
−2081
−2888
−2349
33



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−24
−6764
−7806
−894
−1115
−162
−3231
−8914
−8607


34(E)
652
−2550
1383
2004
−2857
−1868
−643
−2618
−298
−2563
−1660
−497
−2040
1542
−832
−908
985
−2168
−2738
−2028
34



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8604


35(E)
978
−3351
−336
2983
−3871
−2170
−1425
−3692
−1401
−3640
−2859
1765
−2619
−1063
−2043
−1667
−1932
−3175
−3830
−3006
35



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8600


36(P)
−1823
−2153
−3843
−3947
−3271
−2734
−3495
−1581
−3785
−2766
−2465
−3169
3446
−3607
−3696
−2195
−2168
2328
−3859
−3579
36



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8596


37(D)
−2642
−4390
3693
−696
−4628
−2447
−1891
−4714
−2203
−4552
−3980
−1088
−3019
1971
−3045
−2293
−2745
−4173
−4588
−3642
37



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8592


38(V)
−2557
−2122
−5071
−4711
−1962
−4673
−4220
320
−4512
984
−791
−4445
−4483
−4165
−4454
−4020
−2550
3348
−3513
−3210
38



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7945
−8988
−894
−1115
−701
−1378
−8914
−8589


39(V)
−2838
−2616
−4834
−4880
−3213
−3955
−4416
−610
−4757
−2310
−2253
−4383
−4345
−4643
−4642
−3732
−3007
3763
−4157
−3886
39



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−12
−7946
−8988
−894
−1115
−701
−1378
−8914
−8585


40(M)
−3038
−2583
−5400
−4796
1866
−4936
−3671
2288
−4513
1389
3379
−4610
−4379
−3590
−4127
−4157
−2917
−1104
−2668
−2694
40



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8581


41(A)
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
41



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8577


42(C)
2065
2925
−3988
−4043
−3973
1287
−3331
−3726
−3772
−3993
−3088
−2601
−2687
−3352
−3601
2060
−1451
−2653
−4224
−4091
42



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8574


43(A)
2866
2846
−4109
−4001
−2791
−2206
−3077
−1268
−3662
−2419
−1888
−2761
−2871
−3275
−3465
−1533
−1500
1398
−3377
−3097
43



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8570


44(G)
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
44



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8566


45(D)
1008
−3741
3309
−578
−4350
−2242
−1698
−4254
−1941
−4177
−3484
1820
−2795
−1375
−2757
−1930
−2306
−3665
−4371
−3418
45



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8562


46(V)
−1488
−1444
−2805
1912
−1672
−3036
−1995
1499
−2149
−1163
−719
−2290
−3083
−1996
−2352
−2120
998
2130
−2347
−1936
46



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8558


47(P)
−2288
−2497
−3856
−3931
−2960
−3134
−3483
1588
−3705
−2343
−2229
−3416
3829
−3629
−3645
−2690
−2575
−1605
−3649
−3323
47



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8555


48(T)
−1494
−1874
−3613
−3499
−2448
−2472
−2890
−1675
−3034
−1763
2674
−2770
−3069
−2947
−2983
−1828
3524
−1602
−3212
−2888
48



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8551


49(F)
−1077
−1060
−2607
−2006
1617
−2517
−1308
1338
853
1327
−74
−1856
−2554
1561
−1724
−1568
−1013
−416
−1457
−1095
49



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8547


50(E)
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
50



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8543


51(T)
1578
−1365
−3818
−3310
−1660
−3164
−2456
1709
−3026
−1148
−726
−2898
−3288
−2750
−2975
−2334
2031
1609
−2396
−2024
51



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8539


52(M)
−3164
−2670
−5546
−4970
−1172
−5167
−3972
−395
−4712
2611
2735
−4857
−4533
−3759
−4329
−4434
−3047
1340
−2847
−2936
52



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8535


53(A)
3164
−2290
−2449
−2301
−3900
−2380
−2191
−3516
1483
−3644
−2906
−2112
−2939
−1904
−1531
−1776
−1904
−2880
−3730
−3462
53



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8531


54(A)
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
54



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8527


55(V)
950
−941
−2811
−2248
−1031
−2327
−1422
−440
−1962
740
−250
−1933
−2494
−1690
−1964
896
1079
1807
−1521
−1165
55



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8524


56(H)
659
−2340
1265
1241
−2654
−1788
2240
−2406
−89
−2353
−1435
−421
−1907
1509
−604
−738
910
−1959
−2525
−1836
56



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8520


57(M)
905
−1698
−4314
−3724
−1143
−3706
−2623
2145
−3388
1126
3388
−3345
−3578
−2895
−3189
−2841
−1918
−295
−2218
−2005
57



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8516


58(L)
−3094
−2629
−5472
−5009
−1335
−5054
−4133
−266
−4744
2900
−148
−4850
−4577
−3917
−4430
−4403
−3029
1404
−3024
−3024
58



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8512


59(N)
−1718
−2924
−1536
−943
−3401
−2491
2272
−3022
67
−2851
−2042
2480
−2560
1853
2240
−1589
−1584
−2659
−2864
−2426
59



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8508


60(Q)
−1303
−2782
−604
1957
−3121
−2037
−786
−2861
1397
−2771
−1895
1587
−2225
2403
−741
−1147
−1246
−2420
−2908
−2240
60



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8504


61(M)
−836
−1314
1097
−815
1234
−2045
2007
−937
−682
−1198
2233
−976
−2124
1369
−1042
−1018
−776
996
−1675
−1208
61



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8500


62(F)
−1264
−1055
−3592
−2980
2448
801
−1770
1523
−2606
732
−96
−2510
−2888
−2223
−2426
−1975
−1207
1242
−1588
−1251
62



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−267
−7946
−2622
−894
−1115
−701
−1378
−8914
−8496


63(P)
−4031
−3755
−4476
−4814
−5166
−3783
−4422
−5849
−5008
−5560
−5299
−4583
4287
−4894
−4709
−4316
−4383
−5266
−4354
−5078
63



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7693
−8735
−894
−1115
−1509
−624
−8914
−8492


64(E)
−1699
−3425
1700
2340
−3660
−1960
−1101
−3504
−1077
−3405
−2608
1773
−2370
2059
−1764
−1441
−1711
−3013
−3587
−2715
64



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7693
−8735
−894
−1115
−358
−2187
−8914
−8488


65(L)
891
−2044
−4713
−4184
−1318
−4146
−3223
1658
−3880
2504
−198
−3858
−3970
−3332
−3681
−3346
−2320
−337
−2643
−2499
65



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8484


66(R)
−3030
−3584
−3982
−2344
−4551
−3458
−1269
−3800
2924
−3397
−2757
−2153
−3376
−852
3034
−2903
−2656
−3598
−3188
−3160
66



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8480


67(F)
−2326
−1891
−4869
−4440
2685
−4485
−3701
2340
−4251
−921
−863
−4124
−4292
−3931
−4198
−3737
−2296
2290
−3181
−2708
67



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8476


68(R)
−3054
−3592
−3980
−2373
−4560
−3469
−1297
−3823
1781
−3421
−2785
−2179
−3395
−881
3702
−2930
−2684
−3621
−3206
−3181
68



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8472


69(F)
−3145
−2644
−4628
−4516
2787
−4252
−1269
−1824
−4116
−1928
−1754
−3303
−4186
−3281
−3729
−3432
−3069
2671
−580
2537
69



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8468


70(V)
−2450
−1960
−5111
−4795
−2565
−4880
−4855
1914
−4732
−1343
−1286
−4581
−4668
−4656
−4868
−4252
−2448
3379
−4256
−3756
70



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8464


71(N)
−1525
−2287
−1522
−1828
−4053
−2153
−2507
−4149
−2667
−4251
−3455
3942
−2849
−2311
−3012
1045
−1898
−3178
−4209
−3651
71



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8459


72(V)
−2838
−2616
−4834
−4880
−3213
−3955
−4416
−610
−4757
−2310
−2253
−4383
−4345
−4643
−4642
−3732
−3007
3763
−4157
−3886
72



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8455


73(V)
−2243
−1824
−4833
−4454
−2402
−4433
−4113
1762
−4305
−1350
−1201
−4152
−4338
−4160
−4358
−3722
1258
3104
−3760
−3307
73



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8451


74(D)
−2668
−4634
3697
1529
−4809
−2393
−1860
−4835
−2286
−4663
−4116
−996
−2985
−1560
−3281
−2276
−2774
−4274
−4791
−3728
74



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8447


75(L)
−3647
−3060
−5937
−5371
−1118
−5682
−4333
−610
−5087
3001
2533
−5429
−4784
−3925
−4592
−5073
−3493
−1435
−2916
−3081
75



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8443


76(M)
−1722
−1497
−3954
−3355
−1014
848
−2243
1549
−2997
978
3830
−2966
−3285
−2560
−2824
−2460
−1661
−398
−1947
−1696
76



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8439


77(K)
−1675
−2604
−1914
−1147
−3023
−2553
−912
−2542
2655
−2539
−1775
−1279
−2595
−506
1666
−1628
1008
985
−2674
−2295
77



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8435


78(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4358
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
78



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8430


79(Q)
−1357
−2106
−1510
−1196
−2392
−2243
−1288
−2153
−824
371
−1573
−1327
−2535
3535
−1081
1033
−1413
−1913
−2627
−2087
79



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8426


80(P)
−1393
−2284
−1577
−1362
−3557
−2164
−1558
−3266
1432
−3269
−2444
−1443
2774
−1192
−1186
2003
−1560
−2662
−3389
−2924
80



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8422


81(K)
−1010
−2349
−767
1764
−2657
−1927
−589
−2362
1961
301
−1468
−584
−2042
−153
−523
917
−946
−1969
−2533
−1906
81



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8418


82(H)
−956
−2424
−615
1087
−2749
−1853
2292
−2494
2040
−2432
−1522
1288
−1978
−92
−585
753
−897
−2050
−2592
−1916
82



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1873
−7946
−474
−894
−1115
−701
−1378
−8914
−8414


83(G)
−853
−1186
−1419
−1603
−2565
3376
−1668
−2627
−1844
−2759
−2200
−1431
−1859
−1709
−1902
−1054
−1192
−2034
−2282
−2387
83



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−36
−6109
−7151
−894
−1115
−2826
−219
−8914
−8409


84(A)
2862
−488
−1346
−1349
−1807
−785
−1257
−1119
−1298
−1627
−1088
−934
−1428
−1183
−1403
−201
−309
−714
−2107
−1798
84



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−36
−6109
−7151
−894
−1115
−2826
−219
−8914
−8405


85(L)
−1446
−1261
−2639
−2396
−166
−2529
−1687
443
−1969
2652
716
−2264
−2607
−1810
−1925
−2083
−1460
71
−1338
−963
85



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−36
−6109
−7151
−894
−1115
−431
−1952
−8914
−8401


86(E)
−1620
−3077
−205
2781
−2713
−2016
−1027
−3107
−935
−3054
−2295
1905
−2376
−703
−1502
−1416
−1618
−2704
−2909
1972
86



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7696
−8738
−894
−1115
−1502
−628
−8914
−8397


87(H)
−1687
−3020
−524
−508
−3027
−2149
4043
−3137
−478
−3027
−2263
1907
−2446
2055
−794
−1508
−1649
−2742
−2951
−2237
87



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7696
−8738
−894
−1115
−360
−2179
−8914
−8392


88(P)
−2450
−4345
1816
1516
−4562
−2311
−1714
−4514
−2022
−4375
−3745
−914
3318
−1394
−2920
−2098
−2536
−3971
−4556
−3521
88



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8388


89(H)
−2178
−3297
−1451
1311
−3729
−2695
4349
−3419
−165
−3210
−2472
−1349
−2852
−755
1705
−2018
−2038
−3087
−3149
−2733
89



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8384


90(G)
1135
−2508
−960
1390
−3988
2880
−1982
−3774
−2052
−3851
−3054
−1368
−2715
−1683
−2580
−1591
−1835
−3036
−4050
−3424
90



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8379


91(M)
−3620
−3042
−5906
−5341
−1118
−5639
−4298
−608
−5051
2656
3645
−5383
−4766
−3909
−4566
−5021
−3469
−1425
−2906
−3062
91



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8375


92(S)
−1385
−2104
−1840
−2115
−4039
−2094
−2642
−4072
−2802
−4207
−3375
1808
−2809
−2466
−3073
3255
−1780
−3055
−4205
−3726
92



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8371


93(D)
−2954
−4081
3777
−1338
−4930
−2812
−2481
−5106
−2896
−4959
−4423
−1697
1695
−2236
−3689
−2728
−3134
−4489
−4667
−4137
93



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8366


94(R)
847
−2632
−1132
2003
−3046
−2175
−695
−2716
1321
−2604
−1741
−819
−2258
−250
2101
−1179
−1206
−2313
−2696
−2152
94



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8362


95(D)
−2629
−4604
3009
2799
−4770
−2374
−1828
−4782
−2234
−4614
−4051
−973
−2960
−1523
−3216
−2241
−2730
−4224
−4757
−3689
95



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8358


96(F)
−4587
−3562
−5019
−5350
4191
−4887
−1144
−3464
−4916
−2789
−2878
−3547
−4760
−3678
−4301
−4156
−4453
−3634
−393
2478
96



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8353


97(D)
988
−3875
2907
1483
−4100
−2208
−1447
−3972
−1539
−3865
−3114
1567
−2675
−1089
−2294
−1800
−2126
−3467
−4055
−3126
97



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8349


98(S)
1458
−2457
1399
−292
−2897
−1890
−750
−2648
1311
−2615
−1722
−599
−2098
−317
−922
1668
−1053
−2187
−2800
−2113
98



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8344


99(L)
−3562
−3022
−4998
−4839
−384
−4758
−2089
−1358
−4382
2908
−731
−3989
−4502
−3590
−4015
−4103
−3467
−1964
−1321
2369
99



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8340


100(F)
−4720
−3927
−5139
−5454
4513
−4504
−2520
−3938
−5326
−3307
−3429
−4469
−4782
−4575
−4818
−4787
−4810
−4172
−1812
−736
100



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8335


101(T)
−1345
−1936
−3485
−3748
−4307
1297
−3469
−4115
−3845
−4377
−3519
−2741
−2943
−3484
−3725
−1588
3638
−3022
−4411
−4351
101



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8331


102(P)
1040
−1933
−1444
−985
−2825
−1942
−1172
−2498
1349
−2593
−1749
−1119
2171
−794
−1131
−1051
1955
−2025
−2847
−2320
102



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8326


103(D
−2253
−3538
3694
−855
−4532
−2334
−2017
−4570
−2397
−4510
−3869
−1194
−2951
−1736
−3233
1140
−2471
−3857
−4578
−3695
103



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1873
−7946
−474
−894
−1115
−701
−1378
−8914
−8322


104(G
−853
−1186
−1419
−1603
−2565
3376
−1668
−2627
−1844
−2759
−2200
−1431
−1859
−1709
−1902
−1054
−1192
−2034
−2282
−2387
104



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−36
−6109
−7151
−894
−1115
−133
−3504
−8914
−8317


105(K
−2513
−3301
−3024
−1871
−4114
−3126
−1139
−3484
3142
−3184
−2485
−1825
−3101
−717
1860
−2405
1288
−3220
−3066
−2911
105



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8313


106(P)
−3125
−3472
−2885
−3107
−3559
−3370
2901
−4916
−3129
−4732
−4256
−3117
3925
−3250
−3218
−3230
−3395
−4360
−3686
−3131
106



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8308


107(V
−2449
−1961
−5107
−4792
−2559
−4869
−4842
1787
−4726
−1338
−1282
−4575
−4662
−4648
−4859
−4241
−2447
3416
−4245
−3747
107



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8304


108(I)
−2038
−1784
−4073
−3538
−1026
−3659
2506
3064
−3042
1092
−254
−3185
−3570
−2750
−2918
−2809
−1976
−421
−2001
−1514
108



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8299


109(F)
−4720
−3927
−5139
−5454
4513
−4504
−2520
−3938
−5326
−3307
−3429
−4469
−4782
−4575
−4818
−4787
−4810
−4172
−1812
−736
109



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8295


110(N
2479
−1867
−2193
−2351
−4027
1237
−2680
−3819
−2833
−4000
−3118
2632
−2690
−2487
−3076
−1354
−1567
−2811
−4182
−3832
110



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8290


111(Y
−4612
−3570
−5027
−5365
3457
−4900
−1135
−3495
−4928
−2816
−2906
−3544
−4767
−3678
−4307
−4162
−4473
−3659
−383
3895
111



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8988
−894
−1115
−701
−1378
−8914
−8285


112(H
−4836
−4275
−4356
−4629
−3663
−4242
5422
−5932
−4492
−5436
−5298
−4579
−4696
−4620
−4352
−5005
−5030
−5648
−3750
−3259
112



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8281


113(G
1132
−1656
−3630
−3843
−4215
3045
−3388
−4017
−3845
−4265
−3324
−2567
−2709
−3385
−3688
1328
−1497
−2799
−4429
−4315
113



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8276


114(Y
−4604
−3569
−5020
−5357
2093
−4892
−1140
−3492
−4921
−2815
−2905
−3546
−4764
−3679
−4305
−4160
−4468
−3656
−389
4550
114



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8271


115(P)
1686
−1775
−1257
1192
−2259
−1996
−1137
−1747
−939
−2059
−1306
−1082
2013
−833
−1354
−1109
−1078
1053
−2490
−1979
115



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8267


116(W
−911
−1979
1204
−354
−1971
−1917
2044
−1821
−260
−1921
2147
−627
−2020
1682
−714
−875
−851
−1531
3682
−1476
116



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8262


117(D
−1595
−2205
2386
−1132
−2251
−2360
−1616
−1668
−1672
1961
−1361
−1358
−2717
−1399
−2106
−1703
1087
−1603
−2785
−2280
117



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8257


118(I)
−2533
−2040
−5154
−4770
−2133
−4913
−4501
3201
−4662
936
−891
−4572
−4602
−4354
−4667
−4240
−2507
1754
−3764
−3470
118



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8253


119(H
−1221
−2591
−1040
1217
−2989
−2108
3064
−2676
1463
−2566
−1690
1307
−2192
−200
1508
−1095
−1130
−2261
−2664
−2094
119



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7946
−8989
−894
−1115
−701
−1378
−8914
−8248


120(R
862
−2187
−859
−332
−2662
770
−616
−2382
−160
−2365
−1469
−604
−1998
1525
1934
922
−871
−1941
−2551
−1920
120



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7948
−8990
−894
−1115
−701
−1378
−8914
−8244


121(L)
−3417
−2857
−5787
−5262
−1221
−5526
−4333
1616
−5024
2930
−11
−5255
−4747
−3973
−4603
−4899
−3296
−959
−2997
−3107
121



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8238


122(T)
−1746
−1500
−4034
−3432
1750
−3391
−2252
1561
−3078
1096
−52
−3017
−3319
−2616
−2884
−2508
2450
−353
−1923
−1630
122



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8234


123(Y
−4607
−3570
−5022
−5359
2289
−4894
−1139
−3493
−4923
−2816
−2907
−3546
−4765
−3679
−4305
−4161
−4470
−3658
−387
4501
123



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8229


124(D
−1348
−2787
2242
−415
−3129
−2090
2199
−2863
−263
−2769
−1903
−717
−2264
1787
2067
−1197
−1284
−2435
−2889
−2250
124



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8224


125(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
125



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8219


126(T)
−900
−1750
−1012
977
−1985
754
−770
1034
−516
−1791
−1012
−790
1172
−435
−956
−941
1779
−1338
−2179
−1644
126



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−510
−7947
−1783
−894
−1115
−701
−1378
−8914
−8214


127(N
−1735
−2957
−337
−503
−3341
−2022
−1191
−3403
−833
−3305
−2603
3506
−2459
2336
−1181
−1571
−1796
−2939
−3255
−2559
127



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−17
−7453
−8496
−894
−1115
−258
−2612
−8914
−8209


128(H
−1956
−3025
−776
−1013
−3561
1155
4532
−3890
−1721
−3852
−3139
1561
−2848
−1522
−2169
−1872
−2130
−3302
−3700
−2914
128



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8205


129(D
−1326
−2680
2762
−433
−3190
751
−1003
−2946
−718
−2918
−2057
−743
−2295
−594
1178
−1205
1254
−2474
−3108
−2410
129



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8200


130(N
−1803
−2774
−1319
−1257
−3841
1092
−1554
−3580
−862
−3494
−2719
3434
−2788
−1187
1621
−1770
−1905
−3037
−3520
−3054
130



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8195


131(L)
−2659
−2243
−5081
−4521
1845
−4592
−3494
1459
−4246
2227
−106
−4254
−4217
−3540
−3970
−3797
−2575
1425
−2707
−2618
131



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8190


132(H
−1265
−1465
−2433
−2035
−1445
−2449
3775
1372
−1614
−1394
−839
−1901
−2701
−1628
−1732
−1623
2191
−807
−1952
−1432
132



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8185


133(V
−2378
−2013
−4879
−4522
−2020
−4336
−3971
321
−4271
−861
2587
−4174
−4288
−3996
−4221
−3659
−2396
3344
−3447
−3110
133



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8180


134(H
−1298
−2431
−1191
1210
−2723
−2201
3201
−2373
−52
−2378
−1563
−884
−2290
−333
2092
−1222
−1207
912
−2555
−2032
134



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8175


135(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
135



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8170


136(Y
−4791
−3935
−4914
−5231
−815
−4530
−2178
−4357
−5024
−3745
−3807
−4206
−4772
−4328
−4584
−4677
−4837
−4435
−1477
4857
136



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8165


137(R
−2010
−3025
−2206
−1337
−3603
−2773
−965
−3110
2304
−2910
2165
1370
−2777
−536
2519
−1900
−1822
−2804
−2890
−2581
137



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8160


138(E)
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
138



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8155


139(E)
−1541
−3143
1571
2510
−3425
−2060
−1020
−3219
1175
−3136
−2281
−669
−2361
1840
−1368
−1340
−1518
−2751
−3304
−2529
139



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8150


140(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
140



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8145


141(T)
−1448
−2786
1566
−494
−3406
−2031
−1186
−3187
−1023
−3164
−2316
1513
−2396
−794
−1603
1061
2567
−2675
−3364
−2628
141



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8140


142(I)
−1880
−1845
−4196
−3993
−2337
−3273
−3434
2845
−3687
−1437
−1287
−3407
−3652
−3527
−3649
−2612
2766
19
−3410
−3029
142



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8134


143(T)
−1427
−2097
−2121
−2355
−3933
−2165
−2713
−3790
−2759
−4026
−3246
1603
−2869
−2560
−2943
−1604
3622
−2931
−4094
−3692
143



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8129


144(T)
−2430
−2823
−4179
−4462
−4591
−3027
−4037
−4454
−4406
−4707
−4191
−3664
−3724
−4237
−4215
−2689
4010
−3747
−4431
−4588
144



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8124


145(P)
−1505
−2080
−3392
−3633
−4254
−2301
−3425
−4043
−3658
−4296
−3530
−2794
3850
−3427
−3585
−1744
1558
−3083
−4340
−4254
145



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8119


146(F)
−3994
−3227
−4915
−5043
3832
−4698
−1205
−2713
−4616
−2132
2459
−3492
−4563
−3530
−4094
−3916
−3878
−2950
−467
2613
146



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8114


147(D
−2625
−4424
3697
−649
−4717
−2403
−1898
−4810
−2318
−4653
−4097
1859
−2997
−1605
−3282
−2269
−2750
−4226
−4723
−3698
147



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8109


148(M
−3455
−2954
−5636
−5138
−1157
−5278
−4061
−621
−4736
1276
4790
−5054
−4653
−3832
−4350
−4674
−3346
−1354
−2859
−2873
148



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8103


149(M
737
−1039
−2128
−1551
−1071
−2251
−1108
−553
−1284
−925
2340
−1505
−2347
−1148
1957
−1292
992
1190
−1511
−1125
149



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8098


150(V
−1589
−1340
−3842
−3261
−1369
−3310
−2277
1533
−2856
−783
2491
−2892
−3292
−2604
1266
−2434
−1540
2552
−2138
−1796
150



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8093


151(C
−948
2699
−2923
−2314
−734
−2453
−1287
−272
−1969
629
−6
−1970
−2503
2182
−1898
−1524
−890
1281
−1253
1875
151



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8087


152(N
−1513
−2274
−1534
−1835
−4051
−2147
−2506
−4137
−2664
−4241
−3442
3900
−2843
−2309
−3007
1223
−1886
−3166
−4207
−3653
152



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8082


153(Q
−1078
−2597
1398
1298
−2904
570
−660
−2670
1137
−2607
−1702
1544
−2056
1583
−856
−929
−1031
−2214
−2776
−2059
153



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8077


154(M
−2272
−1957
−4590
−3986
−1077
−4004
−2865
1417
−3652
2191
2665
−3646
−3782
−3053
−3407
−3149
1251
−644
−2313
−2179
154



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8071


155(D
−2253
−3537
3692
−855
−4532
−2333
−2017
−4569
−2396
−4510
−3868
−1194
−2951
−1735
−3232
1151
−2470
−3856
−4577
−3695
155



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8066


156(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
156



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8061


157(F)
−4608
−3568
−5026
−5363
3806
−4898
−1136
−3489
−4926
−2811
−2901
−3545
−4766
−3678
−4306
−4161
−4469
−3654
−384
3465
157



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8055


158(H
1044
−2139
−1080
−861
−3017
−1958
3956
−2774
−984
−2841
−1992
1421
−2333
−887
−1434
909
−1252
−2260
−3059
−2457
158



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8050


159(L)
−1995
−1713
−4228
−3647
−1208
−3730
−2645
1439
−3309
2122
−164
−3321
−3602
1641
−3161
−2864
−1930
1443
−2284
−2058
159



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8044


160(A
2383
−1556
−2401
−2072
−2208
−2210
−1881
−1266
−1832
−1939
−1334
−1898
−2639
1793
−2020
−1435
−1318
1882
−2638
−2245
160



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8039


161(I)
855
−2283
−1296
−661
−2565
−2186
−701
1827
1284
−2220
−1411
−884
−2249
1555
1499
−1169
−1126
−1886
−2436
−1937
161



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8033


162(D
−1082
−2449
2531
−267
−2754
−1915
−698
−2479
1332
−2474
2209
−579
−2090
−271
−827
743
−1039
−2075
−2684
−2016
162



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8028


163(V
2572
−1717
−4125
−4110
−2971
−2517
−3473
−716
−3875
−2280
−1954
−3032
−3163
−3569
−3746
−1871
−1754
2604
−3798
−3481
163



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8022


164(M
−2861
−2402
−5299
−4748
−1283
−4880
−3804
1499
−4501
2317
2674
−4552
−4398
−3712
−4200
−4119
−2770
1228
−2863
−2864
164



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8017


165(Q
−2277
−4157
2369
2212
−4337
−2257
−1565
−4246
−1745
−4116
−3418
−845
−2768
2820
−2553
−1949
−2331
−3727
−4303
−3315
165



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8011


166(W
−895
−1130
−1817
−1235
−1105
−2196
2271
−700
−958
627
−314
1121
−2263
−882
1030
−1203
−832
1095
2753
−1082
166



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8989
−894
−1115
−701
−1378
−8914
−8006


167(L)
894
−1665
−4454
−3932
−1615
−3916
−3023
1762
−3653
2037
−555
−3565
−3811
−3286
−3532
−3090
−1967
1475
−2679
−2377
167



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8990
−894
−1115
−701
−1378
−8914
−8000


168(P)
−1358
−1915
1378
−983
−1290
−2313
−1059
−1560
−1164
599
−1093
−1181
2573
−988
−1557
−1458
−1325
−1413
−1772
2001
168



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8990
−894
−1115
−701
−1378
−8914
−7994


169(H
835
−2044
−983
−412
−2272
−1961
2288
−1925
−107
−2006
−1181
−671
−2047
2276
1159
−910
−883
818
−2280
−1718
169



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8990
−894
−1115
−701
−1378
−8914
−7989


170(N
−2018
−3288
1710
−611
−3318
−2277
−1523
−3152
−1621
1866
−2576
2651
−2730
−1220
−2271
−1839
−2066
−2863
−3553
−2765
170



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8990
−894
−1115
−701
−1378
−8914
−7983


171(R
−1939
−2994
−2042
−1272
−3636
697
−982
−3164
2274
−2958
−2181
−1388
1383
−551
2420
−1831
−1784
−2823
−2935
−2603
171



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8990
−894
−1115
−701
−1378
−8914
−7977


172(Y
−813
−1780
−908
−364
−1941
446
−570
−1583
1228
−1731
−928
1199
−1968
−200
−701
−813
1109
664
−2082
1675
172



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−14
−7947
−8990
−894
−1115
−701
−1378
−8914
−7972


173(V
1834
−1515
−2997
−2525
−2124
−2365
−2002
−972
−1828
−1816
−1242
−2181
−2772
−1966
1207
−1599
−1390
2598
−2580
−2228
173



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1214
−7948
−834
−894
−1115
−701
−1378
−8914
−7966


174(N
2098
−1102
−662
−657
−2411
−1122
−1037
−2101
−916
−2331
−1548
2678
−1684
−766
−1264
−457
−604
−1513
−2576
−2066
174



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−26
−6760
−7802
−894
−1115
−2557
−269
−8914
−7960


175(Q
−711
−1121
−1177
−831
−1223
−1760
−770
−238
−424
−821
−384
−912
−1989
2576
−639
−946
−736
2065
−1698
−1211
175



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−26
−6760
−7802
−894
−1115
−2557
−269
−8914
−7954


176(N
−707
−1550
−295
−469
−2833
2212
−1124
−2747
−1097
−2834
−2065
2825
−1857
−854
−1516
−754
−965
−2073
−2881
−2341
176



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−686
−6760
−1453
−894
−1115
−2557
−269
−8914
−7948


177(L)
−1446
−1261
−2639
−2396
−166
−2529
−1687
443
−1969
2652
716
−2264
−2607
−1810
−1925
−2083
−1460
71
−1338
−963
177



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−6111
−7153
−894
−1115
−435
−1941
−8914
−7943


178(C
1406
3275
−2556
−2171
−2790
2067
−1965
−2431
−1828
−2675
−1880
−1777
−2338
−1781
1478
−1008
−1091
−1870
−3029
−2681
178



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7695
−8737
−894
−1115
−1509
−624
−8914
−7937


179(N
1616
−1783
−884
−388
−2121
−1793
−600
−1720
1446
−1882
−1075
1636
−1951
−220
−618
−795
−775
841
−2206
−1651
179



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7695
−8737
−894
−1115
−358
−2187
−8914
−7931


180(H
826
−2030
1249
−286
−2281
−1850
2945
−1963
−256
−2046
2137
−570
−1980
−190
−741
713
−829
−1630
−2333
−1721
180



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7925


181(L)
−1888
−1658
−4011
−3398
1778
−3497
−2279
1355
1112
2128
−19
−3057
−3387
−2573
−2834
−2608
−1812
−657
−1938
−1655
181



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7919


182(C
−1582
2847
−3677
−3112
−1572
−3286
−2308
2397
1781
−1057
−639
−2820
−3303
−2550
−2691
−2415
−1546
1459
−2297
−1922
182



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7913


183(Q
889
−2895
1501
1223
−3185
−1975
−867
−2968
−610
−2899
−2019
1640
−2225
2395
−1182
−1154
−1298
−2505
−3073
−2320
183



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7907


184(M
624
−2075
−807
1189
−2318
−1853
−499
−2000
1304
56
1892
−530
−1940
−75
1281
−778
−783
−1655
−2297
−1689
184



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7901


185(M
−2863
−2466
−4981
−4452
−787
−4528
−2772
−688
−4077
1881
3969
−4038
−4163
−3323
−3759
−3712
−2755
−1286
−2008
2156
185



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7895


186(N
889
−2816
1294
−278
−3142
−1979
−855
−2912
2030
−2849
−1972
2349
−2220
−431
−1093
−1140
−1272
−2455
−3022
−2293
186



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7889


187(W
−912
−2365
1857
1258
−2669
−1819
−525
−2416
−128
−2373
−1466
−456
−1946
−77
1280
718
−855
−1981
2923
−1868
187



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7883


188(K
−1038
−1343
−1852
−1266
1554
−2283
−1007
−874
2350
−1087
2145
−1336
−2361
−894
−1097
−1304
1178
−773
−1689
−1243
188



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7877


189(L)
−2298
−2730
−2950
−2003
−2607
−3127
−1308
−2182
−152
2394
−1514
−1955
−3117
1691
1757
−2379
−2109
−2248
−2689
−2425
189



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7870


190(V
−1079
−1178
−2219
953
−1217
−2479
−1279
1243
1083
512
−362
−1650
−2531
−1279
−1608
−1507
−1021
1902
−1713
−1318
190



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7864


191(E)
−1354
−2910
1356
2455
−3203
−1991
−882
−2982
1322
−2913
−2038
−608
−2244
−460
−1170
−1178
1230
−2523
−3086
−2339
191



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7858


192(H
995
2611
−2882
−2350
−1156
−2330
3755
1388
−2017
−1028
−400
−2003
−2551
−1787
−2019
−1469
−1023
−451
−1652
−1275
192



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7852


193(F)
−873
−2098
−751
1196
2231
−1853
−534
−2026
−137
−2078
−1223
1242
−1958
−115
1091
−800
953
−1680
−2329
−1708
193



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7845


194(Q
684
−2845
−419
1349
−3160
−1991
−875
−2930
−582
−2870
−1997
2370
−2238
2528
−1121
−1166
−1300
−2477
−3045
−2315
194



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7839


195(Y
−3797
−3103
−4834
−4924
2259
−4579
−1182
−2596
−4505
−2328
−2267
−3426
−4481
−3479
−4016
−3789
−3700
1231
−456
4130
195



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7833


196(I)
1842
−1480
−3746
−3421
−2131
−2613
−2685
2759
−3142
−1622
−1194
−2759
−3062
−2873
−3093
−1882
1362
−243
−2830
−2485
196



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7827


197(C
−917
2447
−2927
−2307
−742
−2421
−1266
−267
−1915
−627
2390
−1950
−2475
−1643
1928
−1495
−858
1278
−1243
1688
197



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7820


198(E)
−2062
−3846
1749
2650
−4068
−2200
−1423
−3938
−1496
−3829
−3070
1774
−2658
−1061
−2241
835
−2093
−3434
−4016
−3094
198



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7814


199(H
−1285
−2612
−523
1316
−2833
−2011
3126
−2579
−558
−2606
−1772
2447
−2236
−462
−1057
−1160
−1248
676
−2824
−2144
199



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7807


200(G
−2654
−3212
−2507
−2588
−4732
3403
−2707
−4668
1545
−4548
−3892
−2604
−3567
−2456
−2072
−2733
−2883
−4011
−4170
−4141
200



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7801


201(Y
−818
−1798
1165
976
−1948
−1871
−567
748
−261
−1738
−935
−608
−1966
1445
−722
−813
−758
820
−2091
1672
201



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7794


202(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
202



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7788


203(M
−2141
−1848
−4423
−3825
−1086
−3850
−2726
2359
−3484
1059
3290
−3479
1362
−2946
−3270
−2988
−2066
−538
−2251
−2080
203



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7781


204(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
204



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7775


205(E)
−2636
−4572
1810
3431
−4760
−2390
−1841
−4772
−2235
−4607
−4040
−995
−2973
−1537
−3199
−2253
−2735
−4218
−4733
−3691
205



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8990
−894
−1115
−701
−1378
−8914
−7768


206(I)
−2944
−2476
−5069
−4784
2837
−4680
−2731
3180
−4519
−584
−703
−4172
−4449
−3817
−4249
−3998
−2901
−494
−1967
−1074
206



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8991
−894
−1115
−701
−1378
−8914
−7762


207(T)
−1149
−1001
−3245
−2651
−830
−2680
−1537
−200
−2295
678
−115
−2252
−2729
−1967
−2187
−1772
2521
1202
−1425
1941
207



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8991
−894
−1115
−701
−1378
−8914
−7755


208(N
968
−3781
2422
1475
−4014
−2190
−1399
−3875
−1453
−3773
−3006
2431
−2639
−1035
−2184
−1745
−2052
−3376
−3962
−3054
208



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8991
−894
−1115
−701
−1378
−8914
−7748


209(W
1214
−2627
−4165
−4274
−2248
−2999
−2977
−3589
−3845
−3575
−3208
−3402
−3626
−3726
−3688
−2549
−2659
−3197
5855
−1919
209



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7948
−8991
−894
−1115
−701
−1378
−8914
−7741


210(V
−1358
−2495
−1397
−737
−2853
−2276
−735
−2472
1340
−2436
−1616
−951
1242
1587
1414
−1276
−1250
1808
−2580
−2097
210



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7949
−8991
−894
−1115
−701
−1378
−8914
−7735


211(W
−3498
−2927
−4676
−4658
236
−4377
−1183
−2501
−4186
−2296
−2147
−3324
−4322
−3336
−3785
−3580
−3418
1241
5352
2614
211



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−16
−7949
−8991
−894
−1115
−701
−1378
−8914
−7728


212(H
−955
−1646
−1373
−926
1547
635
2162
−1632
−859
−1839
−1087
−1075
2103
−763
−1254
967
−989
−1372
−2122
−1543
212



*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*



*
*
*
*
*
*
*
−8914
0


















TABLE 14









HMMER2.0 [2.2g]



NAME PTA_PTB_exp_seqs



LENG 355



ALPH Amino



RF no



CS no



MAP yes



COM /app/public/hmmer/current/bin/hmmbuild PTA_exp_hmm PTA_PTB_exp_seqs.aln



COM /app/public/hmmer/current/bin/hmmcalibrate PTA_exp_hmm



NSEQ 10



DATE Mon Nov 30 18:53:24 2009



CKSUM 2317



XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4



NULT −4 −8455



NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 384 −1998 −644



EVD −321.869965 0.133020

































A
C
D
E
F
G
H
I
K














HMM
m->m
m->i
m->d
i->m
i->i
d->m
d->d
b->m
m->e
L
M
N
P
Q
R
S
T
V
W
Y






−438
*
−1933


1(M)
−1511
−1258
−3275
−2807
2269
−2995
−1346
266
−2474
800
3792
−2446
−2858
−1968
−2335
−2172
−1449
−138
−793
−87
26



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−23
−6577
−7619
−894
−1115
−701
−1378
−438
*


2(M)
426
−1446
−3780
−3363
−1521
−2856
−2514
−90
−3024
−729
4481
−2828
−3133
−2721
−2939
−2091
−1564
1100
−2424
−2124
27



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−10
−7762
−8804
−894
−1115
−701
−1378
*
*


3(D)
−898
−2223
2613
−202
−2512
−1832
−557
−2225
779
−2241
1722
−504
−1962
−125
−678
389
566
−1836
−2468
−1816
28



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


4(L)
−3343
−2811
−5687
−5115
2061
−5345
−3911
1769
−4867
2527
56
−5003
−4615
−3800
−4424
−4629
−3207
−1173
−2733
−2675
29



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


5(F)
−3039
−2583
−5400
−4800
2731
−4939
−3665
2351
−4517
1482
1999
−4610
−4383
−3595
−4132
−4161
−2919
−1092
−2663
−2673
30



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


6(E)
−1913
−3483
−329
3240
−3847
−2201
−1343
−3669
−1205
−3580
−2799
−844
−2614
1375
−1770
328
−1937
−3187
−3742
−2936
31



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


7(K)
−892
−2327
−754
−192
−2667
−1850
−472
−2397
1835
−2332
−1421
831
−1938
1186
1037
1297
497
−1961
−2483
−1835
32



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


8(I)
514
−1727
−4577
−4078
−1745
−4080
−3252
2588
−3824
1712
−655
−3731
−3953
−3476
−3724
−3273
−2060
1203
−2879
−2568
33



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


9(W)
681
−1696
−954
−400
531
−1888
−578
324
1375
−1628
−839
−651
−1974
1030
909
344
−741
−1214
2014
−1462
34



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


10(E)
−1665
−3313
1727
2676
−3578
225
−1130
−3392
−1004
−3309
−2472
−694
−2435
928
−1638
306
−1659
−2915
−3488
−2672
35



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


11(K)
−1580
1884
−1764
−1020
−2907
−2481
−845
−2479
1956
772
−1679
−1179
−2514
1923
1777
−1525
−1447
−2203
−2604
−2197
36



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


12(A)
2315
−1624
−4424
−3936
−1894
−3936
−3165
1771
−3688
61
−834
−3585
−3870
−3418
−3634
−3127
−1946
1865
−2913
−2538
37



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


13(K)
240
−2148
−1205
−600
−2451
−2088
−695
−2066
2741
−2143
−1331
−829
−2179
−293
688
−1066
326
519
−2394
−1872
38



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


14(Q)
251
−2245
585
−74
−2564
1048
−408
−2314
875
−2260
−1335
−384
515
1120
955
394
−715
−1866
−2429
−1747
39



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


15(D)
511
−2241
1741
−132
−2549
−1792
−445
−2281
1286
455
−1337
−435
−1886
971
685
−711
−760
−1857
−2426
−1765
40



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


16(K)
−884
−2341
1633
−125
−2653
223
−507
−2400
1752
−2356
−1445
919
707
−57
−629
−761
−829
80
−2535
−1850
41



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


17(K)
535
−1741
−1193
−620
−1889
−2043
−709
327
2612
−1654
−903
−840
−2126
854
−679
−1007
−888
295
−2069
−1566
42



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


18(K)
−2416
−3238
−2867
−1785
−4015
−3059
−1120
−3414
2580
−3139
−2429
−1764
−3047
−698
2089
−2312
2234
−3140
−3041
−2859
43



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


19(I)
−2521
−2024
−5153
−4783
−2223
−4929
−4595
3447
−4687
294
−972
−4589
−4629
−4430
−4724
−4267
−2498
1528
−3873
−3540
44



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


20(V)
213
−1847
−4873
−4520
−2465
−4420
−4247
1419
−4388
−1381
−1247
−4198
−4362
−4252
−4452
−3727
−2270
3336
−3880
−3419
45



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


21(F)
−3693
−3087
−5586
−5265
3261
−5304
−2888
−912
−4973
2381
−226
−4694
−4708
−3838
−4472
−4669
−3558
−1678
−1934
−1153
46



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


22(P)
1357
−1977
−3526
−3796
−4305
−2211
−3504
−4098
−3878
−4361
−3542
−2788
3823
−3529
−3749
−1636
−1847
−3043
−4411
−4353
47



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


23(E)
−2645
−4564
1495
3508
−4762
−2401
−1853
−4778
−2245
−4613
−4046
−1009
−2982
−1550
−3202
−2265
−2745
−4225
−4728
−3699
48



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


24(G)
828
−1562
−2312
−1921
−2620
2562
−1856
−2263
−1813
−2508
−1731
−1730
−2452
−1660
−2098
554
782
−1818
−2902
1503
49



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


25(E)
−1027
−2214
−837
2256
−2461
−1966
−641
−2117
974
634
−1343
−652
−2082
−229
−588
−955
1152
−1790
−2438
−1847
50



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


26(W)
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
51



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


27(D)
−2650
−4626
3535
2005
−4794
−2383
−1844
−4814
−2264
−4643
−4090
−983
−2972
−1542
−3255
−2259
−2754
−4254
−4780
−3711
52



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


28(E)
−884
−1962
−861
2360
−2149
−1901
−576
272
764
−268
−1086
−611
909
−182
−632
−849
−822
−1489
−2226
−1648
53



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


29(R)
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
54



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


30(V)
−1615
−1406
−3692
−3190
−1680
−3315
−2460
2105
−2933
−1136
−730
1527
−3366
−2700
−2939
−2463
576
2489
−2439
−2054
55



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


31(L)
−2893
−2389
−5409
−4931
−1519
−5116
−4253
2238
−4753
2516
−300
−4794
−4601
−4030
−4524
−4423
−2823
819
−3207
−3187
56



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


32(K)
−810
−2256
−666
736
−2577
491
−428
19
1965
−2266
−1348
−419
−1872
1176
702
323
−747
−1879
−2434
−1767
57



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


33(A)
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
58



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


34(A)
2937
−1528
−3502
−3308
−3125
−1952
−2769
−2630
−3081
−3039
−2271
−2390
−2643
−2779
−3078
549
1011
493
−3475
−3206
59



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


35(H)
804
−2234
624
1374
−2527
−1779
1863
−180
−65
−2239
−1335
−424
−1886
−14
−572
1383
−764
−1842
−2435
−1765
60



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


36(K)
−927
−1951
−1003
613
−2143
−1960
−589
446
1801
−1893
1449
−680
−2039
−201
1687
−903
−856
−1504
−2198
1303
61



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


37(I)
−3179
−2647
−5611
−5124
−1356
−5341
−4317
2901
−4907
2264
−148
−5052
−4698
−4019
−4582
−4697
−3088
−546
−3099
−3137
62



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


38(I)
1305
−916
−2346
−1758
−894
−2325
−1140
1372
98
911
−104
−1638
−2383
1135
−1607
−1364
−839
747
−1366
−994
63



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


39(K)
103
−2280
−655
781
−2607
−33
−431
−2352
2090
−2292
−1371
817
−1875
1393
703
−697
−754
−1906
−2454
−1782
64



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


40(D)
−876
−2355
1990
1398
−2673
−1797
−489
−2425
861
−2368
−1450
862
−1916
−35
739
−749
−818
−1976
−2535
1245
65



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


41(G)
−1910
−3521
1036
−459
−3833
2939
−1305
−3641
669
−3546
−2760
−819
−2598
922
−1710
−1672
−1918
−3170
−3710
−2907
66



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


42(L)
−2752
−2316
−5181
−4626
1316
−4723
−3629
2215
−4364
2228
−104
−4388
−4302
−3622
−4077
−3942
−2665
822
−2775
−2716
67



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


43(A)
2595
−1592
−4065
−3655
−1874
−3190
−2875
69
−3364
237
−870
−3140
−3438
−3084
−3305
−2430
−1771
2003
−2788
−2442
68



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


44(K)
−781
−2248
1290
1182
−2566
−1751
−412
−18
1303
−2262
−1338
836
−1846
47
956
−662
674
−1869
−2432
−1751
69



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


45(P)
−1999
−1685
−4334
−3818
−1612
−3852
−2976
1828
−3535
394
−557
−3486
3050
−3199
−3450
−3026
−1964
1176
−2676
−2369
70



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


46(V)
−2452
−1958
−5119
−4802
−2578
−4903
−4884
2288
−4745
−1352
−1293
−4595
−4680
−4675
−4887
−4275
−2449
3237
−4279
−3776
71



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


47(L)
−2998
−2538
−5403
−4947
−1395
−4989
−4115
−173
−4693
2765
−209
−4769
−4551
−3928
−4414
−4328
−2940
1827
−3062
−3033
72



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


48(V)
−2510
−2021
−5126
−4737
−2130
−4872
−4437
1947
−4622
1286
−893
−4529
−4574
−4319
−4624
−4190
−2483
2866
−3732
−3431
73



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


49(G)
931
−1908
−3564
−3879
−4400
3463
−3561
−4237
−4052
−4490
−3601
−2764
−2927
−3600
−3877
−1557
−1777
−3055
−4501
−4478
74



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


50(N)
91
−2861
975
−587
−3637
−2069
−1361
−3439
−1272
−3412
−2579
2972
−2496
−985
−1877
1896
−1654
−2876
−3606
−2851
75



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


51(E)
−1387
−2840
−463
2722
−3195
−2028
−944
−2952
−645
−2907
−2050
−684
1763
936
−1162
−1231
313
−2508
−3091
−2377
76



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


52(N)
1378
−2248
457
624
806
−1762
−434
−2299
865
−2258
−1342
1494
−1863
20
−534
341
−740
−1863
−2438
−1760
77



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


53(E)
975
−2349
734
2085
−2655
−1807
−516
−2399
1272
−2359
−1451
−445
−1934
−67
−640
−772
−840
10
−2541
−1856
78



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


54(I)
−2526
−2030
−5153
−4776
−2181
−4921
−4549
3338
−4675
616
−934
−4580
−4616
−4394
−4697
−4253
−2501
1644
−3821
−3507
79



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


55(K)
398
−2606
−1173
479
−3022
−2165
−660
−2692
2540
−2573
−1705
−805
−2232
1750
1236
−1146
−1168
−2286
−2659
−2117
80



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


56(E)
1257
−2318
−622
1753
−2641
−1799
−475
−2386
1275
−2333
−1417
−437
−1908
1398
−542
391
−801
−1943
−2501
−1824
81



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


57(F)
471
−982
−1994
−1417
1160
−2213
−1003
−523
1101
784
−172
584
−2279
−1043
−1416
−1230
532
1019
−1408
−1017
82



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


58(A)
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
83



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


59(K)
252
−2225
594
−101
−2531
1206
−438
−2270
1451
−150
−1326
673
−1865
14
−536
340
−739
−1841
−2425
−1752
84



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−299
−7942
−2449
−894
−1115
−701
−1378
*
*


60(E)
1167
−2286
732
1831
−2598
−1667
−407
−2354
951
−2301
−1391
−300
−1816
40
−560
783
−749
−1906
−2475
−1775
85



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7654
−8696
−894
−1115
−1585
−585
*
*


61(H)
−815
−727
−2621
−2031
1145
−2289
2094
−200
−1722
1681
107
494
−2340
−1435
−1697
−1354
−755
1054
−1030
−564
86



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−11
−7654
−8696
−894
−1115
−337
−2262
*
*


62(N)
−1704
−3281
1777
−397
−3622
1646
−1188
−3429
−1036
−3352
−2526
2598
−2480
−796
1047
−1495
−1710
−2949
−3526
−2731
87



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


63(M)
20
−1214
−3805
−3190
774
−3078
−1973
1042
−2815
1458
2157
−2723
−3056
−2395
−2615
−2183
−1382
1944
−1731
−1436
88



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


64(D)
605
−2467
2563
−401
−3040
−1918
−912
−2798
−624
−2777
−1892
929
−2187
−492
−1157
1186
553
−2307
−2970
−2279
89



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


65(L)
−3447
−2885
−5829
−5243
−1145
−5544
−4253
1795
−5014
2666
2377
−5248
−4707
−3887
−4549
−4864
−3303
−1162
−2916
−3088
90



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


66(D)
250
−2410
1559
1458
−2748
39
−576
−2502
−207
−2454
−1544
−475
−1976
−130
−729
1014
986
−2052
−2631
−1936
91



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


67(G)
−2653
−3216
−2503
−2570
−4722
3367
−2672
−4647
1672
−4521
−3864
−2587
−3560
−2414
−2013
−2728
−2874
−3999
−4152
−4117
92



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


68(V)
1628
−1694
−4600
−4141
−2065
−4161
−3476
2051
−3925
24
−959
−3809
−4058
−3687
−3896
−3377
−2060
2431
−3181
−2784
93



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


69(Q)
−1107
−2491
−869
2104
−2843
−2005
−610
−2548
1501
−2474
1449
−645
−2106
2280
−390
−986
−1031
−2135
−2613
−2004
94



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


70(I)
−2462
−1971
−5120
−4803
−2532
−4890
−4848
3484
−4737
−1301
−1256
−4591
−4671
−4645
−4865
−4262
−2459
1950
−4227
−3745
95



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


71(V)
−1021
−845
−3291
115
−783
−2586
−1463
1772
−2289
711
1731
−2204
−2623
−1926
−2122
−1672
−963
1810
−1336
1149
96



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


72(D)
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
97



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


73(P)
−1143
−1675
−3005
−2989
−3065
−2064
−2700
565
−2869
−2950
−2300
−2338
3698
−2683
−2908
587
−1504
−2001
−3467
−3104
98



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


74(H)
1253
−2202
−717
1654
−2478
−1838
2550
−2187
868
−372
−1310
−496
−1938
−63
−532
−776
−809
−1802
−2405
−1767
99



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


75(T)
−1143
−1868
−2054
−2081
−3765
−1965
−2374
−3533
−2354
−3699
−2834
2518
−2626
−2132
−2643
882
2968
−2663
−3905
−3493
100



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


76(Y)
−1148
−1566
−1831
−1449
−1231
−2241
−1207
−1418
−1361
−1676
−1002
1156
−2489
−1227
−1649
1580
−1191
429
−1711
3317
101



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


77(W)
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
102



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


78(E)
−1130
−2548
993
1837
−2823
−1914
−731
−2562
−425
645
−1679
−557
1649
−306
−961
330
−1088
−2151
−2765
−2070
103



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


79(K)
705
−2293
−914
−357
−2734
819
−598
−2445
2189
−2399
−1507
−628
−2037
−153
975
617
−923
−2013
−2558
−1945
104



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1567
−7942
−603
−894
−1115
−701
−1378
*
*


80(E)
−1455
−2350
50
3362
−2809
−1547
−927
−2700
−845
−2735
−2178
−487
−2000
−660
−1278
−1311
−1525
−2365
−2625
−2220
105



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−26
−6400
−7442
−894
−1115
−1808
−485
*
*


81(M)
−551
−928
1713
−589
−942
−1741
−615
5
−559
−521
2643
−741
−1867
−429
−905
−804
−519
1266
−1450
−997
106



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−19
−6823
−7865
−894
−1115
−167
−3194
*
*


82(K)
−936
−1587
−1304
−718
−1678
−2068
−727
−1273
1622
−91
1465
−909
−2141
−440
1553
−1037
1209
−1083
−1910
1220
107



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


83(Q)
216
−3288
1080
1963
−3557
−2088
−1124
−3366
−985
−3287
−2450
−697
−2432
3156
−1608
−1439
−1648
−2893
−3467
−2658
108



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


84(E)
661
−2268
1307
1400
−2586
−40
−425
−2337
−12
−2283
−1359
−392
−1857
1107
956
−675
492
−1889
−2452
−1768
109



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


85(M)
−2903
−2473
−4675
−4372
1750
−4229
−1624
−1350
−3989
445
3626
−3447
−4067
−3192
−3628
−3390
−2808
−1716
−937
3375
110



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


86(A)
1901
−908
−3172
−2568
−899
−2621
1244
994
−2224
146
−105
−2185
−2665
−1906
−2124
−1705
−1008
1900
−1447
−1095
111



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


87(Q)
−1463
−3014
2011
−328
−3316
−2053
−949
−3092
−641
−3010
−2150
2270
−2319
2525
696
−1279
−1430
−2636
−3169
−2433
112



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


88(A)
2248
−2123
670
−520
−2701
−1892
−856
−2400
−496
−2451
−1589
−769
−2126
−446
1017
−948
1463
−1971
−2692
−2080
113



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


89(F)
−2727
−2338
−4825
−4352
3286
−4296
−2265
816
−3996
1550
−226
−3736
−4033
−3222
−3652
−3458
−2628
−1306
3005
−774
114



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


90(Y)
−2126
−1823
−4287
−3802
−723
−3742
−2094
−327
−3430
1159
−480
−3251
−3663
−2945
−3227
−2891
−2068
2283
−1586
3201
115



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


91(E)
703
−3188
−772
3501
−4265
−2379
−2017
−4040
−2108
−4114
−3438
−1356
−2948
−1721
−2684
−2006
−2303
−3449
−4241
−3565
116



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


92(R)
−2413
−2234
−4171
−3369
−1308
−3884
−2460
877
−2018
2018
−224
−3190
−3714
−2356
2764
−3076
−2310
−961
−2462
−2283
117



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


93(R)
738
−2612
−3347
−2738
−4144
−2736
−2052
−3768
−900
−3705
−3029
−2409
−3189
−1725
3817
−2171
−2228
−3209
−3627
−3498
118



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


94(K)
503
−2445
−1015
−703
−3184
562
−1018
−2893
2978
−2852
−1992
704
−2331
−602
−835
−1206
−1297
−2406
−2999
−2416
119



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


95(H)
−928
−2405
−563
997
−2720
1543
2401
−2472
959
−2417
−1504
868
−1955
−84
−643
392
−872
−2026
−2586
−1897
120



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


96(K)
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
121



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−809
−7942
−1234
−894
−1115
−701
−1378
*
*


97(G)
−2666
−2751
−3377
−3700
−4393
3753
−3528
−4841
−4031
−4741
−4286
−3394
−3411
−3824
−3848
−2912
−3053
−4080
−3719
−4290
122



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7148
−8190
−894
−1115
−541
−1677
*
*


98(M)
412
−754
−2560
−1966
−741
−2291
−1150
981
−1684
−598
3179
1245
−2352
−1421
−1700
−1351
649
877
−1235
−874
123



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−10
−7762
−8804
−894
−1115
−413
−2007
*
*


99(T)
−2430
−2823
−4179
−4462
−4591
−3027
−4037
−4454
−4406
−4707
−4191
−3664
−3724
−4237
−4215
−2689
4010
−3747
−4431
−4588
124



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


100(P)
270
−1065
−2103
243
−1059
−2317
−1151
1403
−1372
790
−240
−1539
2257
−1198
−1557
−1358
−904
−375
−1531
−1145
125



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


101(E)
−2392
−4275
1163
3312
−4486
−2295
−1667
−4423
−1934
−4288
−3633
−894
1125
−1341
−2802
−2049
−2468
−3888
−4470
−3454
126



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


102(Q
297
−2471
1012
−160
−2788
−1849
−577
−2543
1870
−2482
−1573
944
−1994
2462
−693
−848
−930
−2093
−2649
−1955
127



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


103(A
3227
−1594
−3807
−3849
−3646
−1945
−3198
−3067
−3621
−3576
−2783
−2572
−2704
−3241
−3481
576
−1455
241
−3977
−3779
128



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


104(D
1261
−2267
1791
738
−2557
−1813
−517
−2280
−130
−2273
−1380
−464
−1934
−76
1383
−771
−825
386
−2479
−1813
129



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


105(K
415
−2546
−735
1917
−2896
−1973
−635
−2621
2122
−2543
−1653
−608
−2101
1705
−517
−981
−1047
−2189
−2683
−2042
130



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


106(M
308
−827
−2563
−1964
−795
−2335
−1167
1419
607
−677
2765
−1747
−2393
−1425
−1700
−1388
1409
−241
2166
−912
131



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


107(M
−2838
−2385
−5273
−4720
−1280
−4846
−3769
1119
−4469
2241
2938
−4517
−4377
−3690
−4172
−4080
−2748
1487
−2847
−2840
132



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


108(K
−1031
−2407
−918
1080
−2761
−1972
−550
−2464
1949
−434
−1505
−616
−2053
−100
1475
1180
−952
−2050
−2533
−1929
133



−149
−500
233
43
−381
400
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−169
−3204
−8984
−206
−2913
−701
−1378
*
*


109(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
135



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


110(P)
−893
2081
−1781
940
−1121
−2183
−978
444
−1070
−1013
−334
−1295
2567
−933
−1326
−1202
−842
−568
−1538
1138
136



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


111(N
−1006
−916
−2854
−2266
−891
−2503
−1385
865
−1966
1747
−97
1859
−2563
−1688
−1947
−70
−954
854
−1419
−1063
137



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


112(Y
−4083
−3282
−4926
−5082
1832
−4728
−1188
−2828
−4652
−2243
2245
−3495
−4595
−3549
−4122
−3951
−3965
−3052
−449
4351
138



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


113(F)
−4576
−3559
−5015
−5343
4263
−4881
−1152
−3449
−4910
−2776
−2865
−3550
−4757
−3679
−4300
−4154
−4444
−3622
−401
2124
139



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


114(G
2612
−1827
−3654
−3943
−4336
2661
−3533
−4149
−4027
−4411
−3508
−2727
−2864
−3570
−3838
−1474
−1691
−2967
−4475
−4432
140



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


115(T)
1598
−1522
−3726
−3555
−2787
−2149
−2868
−1443
−3288
−2470
−1892
−2585
−2799
−2974
−3213
−1463
2927
1522
−3313
−3022
141



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


116(M
845
−1514
−3507
−3305
−2427
−2152
−2615
−1675
−2968
−2025
4655
−2481
−2760
−2728
−2908
−1457
695
−1444
−2989
−2699
142



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


117(M
103
−2295
−4810
−4261
−1123
−4222
−3233
−505
−3905
1982
4088
−3963
−4004
−3263
−3656
−3428
−2527
−1018
−2542
−2485
143



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


118(V
249
−1764
−4559
−4171
−2124
−3892
−3557
400
−3936
216
−1010
−3749
−3970
−3700
−3906
−3157
−2087
3302
−3297
−2917
144



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


119(Y
−1748
−2787
−1867
−1117
−3134
−2580
−896
−2823
2430
−2708
−1928
−1262
−2616
−482
1298
588
−1603
−2513
−2720
2852
145



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


120(M
543
−1502
454
−655
−1569
−2005
−725
−1152
1280
1097
2617
−860
−2092
−451
−910
−972
−801
−975
−1856
−1371
146



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


121(G
−2913
−3668
945
−1911
−5049
3566
−2931
−5265
−3381
−5155
−4587
−2191
−3583
−2748
−4001
−2853
−3188
−4499
−4671
−4461
147



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


122(E)
418
−2234
1260
1822
−2533
−1769
−443
−2271
1177
−485
−1330
−412
−1871
9
−542
−693
−747
−1845
−2428
811
148



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


123(V
2475
−1721
−4141
−4120
−2951
−2548
−3488
−657
−3886
−2245
−1927
−3055
−3187
−3584
−3760
−1902
−1766
2703
−3796
−3475
149



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


124(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
150



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


125(G
−1595
−2107
−3720
−3963
−3970
3471
−3570
−3119
−3981
−3886
−3293
−2985
−3155
−3665
−3837
−1865
−2027
946
−4215
−4068
151



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


126(M
−2025
2478
−4388
−3797
−1130
−3739
−2667
−158
−3453
1208
4105
−3402
−3608
−2937
−3236
−2881
−1961
880
−2231
−2028
152



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


127(W
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
153



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


128(V
−2447
−1962
−5103
−4789
−2553
−4857
−4827
1666
−4719
−1334
−1279
−4568
−4656
−4638
−4849
−4228
−2447
3448
−4233
−3737
154



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


129(S)
2131
−1670
−3692
−3910
−4174
−1935
−3404
−3963
−3845
−4238
−3317
−2599
−2728
−3415
−3673
2964
−1517
−2788
−4397
−4269
155



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


130(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
156



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


131(A
3075
−1760
−2603
−2695
−3957
−1941
−2800
−3736
−2952
−3942
−3051
1370
−2670
−2640
−3110
792
−1502
−2723
−4139
−3839
157



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


132(V
1970
−1618
−4439
−3946
−1865
−3942
−3147
1777
−3693
528
−807
−3588
−3866
−3412
−3626
−3130
−1942
2096
−2880
−2511
158



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


133(H
−1102
2379
−1738
−1466
−2663
−2010
4358
−2506
−1383
−2678
−1892
969
−2465
−1337
−1706
783
−1293
−2046
−2891
−2346
159



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


134(S)
419
−1643
−3250
−3104
−3814
−1905
−2857
−3555
−2981
−3781
−2885
−2311
2298
−2716
−3080
2397
1614
−2583
−4014
−3764
160



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


135(T)
−1102
−1714
−3480
−3700
−4076
−1973
−3311
−3843
−3648
−4140
−3265
−2565
−2755
−3310
−3525
656
3729
−2765
−4307
−4127
161



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


136(G
2443
−1650
−3680
−3879
−4195
2494
−3389
−3992
−3844
−4243
−3304
−2574
−2707
−3388
−3682
672
−1492
−2786
−4412
−4299
162



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


137(D
−2610
−4414
3375
−640
−4704
−2395
−1885
−4787
−2296
−4632
−4069
2731
−2988
−1589
−3253
−2255
−2733
−4206
−4710
−3683
163



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


138(T)
−1802
−1610
−4107
−3628
−1625
−3434
−2777
121
−3310
979
−612
−3213
−3529
−3008
−3231
−2634
2771
1888
−2588
−2269
164



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


139(L)
−2900
−2445
−5330
−4886
−1470
−4921
−4106
−73
−4643
2578
−286
−4689
−4527
−3948
−4403
−4253
−2850
2224
−3114
−3047
165



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


140(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
166



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


141(P)
1695
−1951
−3539
−3802
−4288
−2189
−3492
−4075
−3870
−4341
−3515
−2772
3709
−3517
−3739
−1609
−1819
−3017
−4404
−4341
167



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


142(A
2997
−1820
−3672
−3957
−4327
2092
−3531
−4137
−4026
−4400
−3498
−2726
−2859
−3569
−3834
−1467
−1684
−2957
−4469
−4425
168



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


143(L)
−3221
−2748
−5558
−5092
−1274
−5134
−4164
−386
−4812
3030
−86
−4956
−4613
−3913
−4456
−4503
−3148
772
−2987
−3018
169



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


144(Q
−2871
−3512
−2899
−2161
−4235
−3278
−1502
−3869
−191
−3535
−2901
−2118
−3368
4099
1519
−2774
−2657
−3608
−3317
−3159
170



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


145(I)
−2460
−1967
−5122
−4804
−2546
−4899
−4864
3420
−4742
−1315
−1266
−4595
−4676
−4657
−4875
−4271
−2457
2106
−4245
−3757
171



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


146(I)
−2482
−1984
−5127
−4768
−2335
−4912
−4643
3097
−4684
313
−1075
−4568
−4633
−4487
−4753
−4252
−2463
2302
−3978
−3593
172



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


147(K
−2698
−3306
−2555
−2385
−4613
1689
−2226
−4379
3466
−4176
−3510
−2406
−3487
−1898
−1272
−2718
−2789
−3870
−3879
−3804
173



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


148(T)
−1775
−2078
−3737
−3648
−2269
−2758
−3026
−1461
−3198
577
−1417
−3004
−3304
−3108
−3140
−2144
3610
−1504
−3171
−2809
174



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


149(K
1442
−2243
−1132
−565
−2592
−2073
−704
−2218
2425
−2266
−1439
−805
−2179
1424
−419
−1063
−1056
139
−2490
−1948
175



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


150(P)
−2229
−3531
−751
745
−4074
−2482
−1471
−3788
860
−3650
−2934
−1174
3599
−1104
−1174
−2025
−2220
−3370
−3701
−3125
176



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


151(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
177



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


152(C
1031
2336
−3000
−2410
−865
−2361
−1377
−341
−2075
407
−99
−2001
−2486
−1756
−1986
688
649
1750
−1361
−1013
178



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


153(K
299
−2345
−751
−248
−2698
−1882
2125
−2424
2532
−2376
−1479
830
−1996
−103
−500
327
−894
−1997
−2541
−1898
179



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


154(T)
−908
−953
−2280
−1684
−935
−2313
−1108
722
344
1215
−140
−1590
−2372
−1223
1008
−1350
1552
668
−1392
−1018
180



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


155(V
248
−1426
−4165
−3634
−1671
−3597
−2693
2073
−3341
492
−700
−3234
−3564
−3048
−3243
−2750
488
2467
−2499
−2124
181



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


156(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
182



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


157(G
693
−1658
−3600
−3793
−4209
2495
−3362
−4011
−3794
−4256
−3314
−2553
−2706
−3347
−3658
2479
−1495
−2797
−4419
−4298
183



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


158(F)
757
−848
−3195
−2588
2258
−2533
−1456
1163
−2229
−678
−69
−2151
−2599
−1889
−2097
1006
−949
1406
−1371
−1020
184



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


159(F)
−3561
−3044
−5093
−4992
4144
−4702
−2042
−1417
−4612
−651
2173
−3997
−4516
−3679
−4183
−4055
−3507
−1990
−1263
−230
185



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


160(I)
−2399
−1985
−4894
−4389
1240
−4436
−3536
2456
−4148
1300
−435
−4090
−4179
−3651
−3986
−3652
−2345
2073
−2927
−2716
186



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


161(M
−2968
−2569
−5237
−4809
−1359
−4763
−3886
1091
−4419
−34
4848
−4576
−4450
−3792
−4170
−4116
−2930
−673
−2953
−2839
187



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


162(Q
309
−798
−2699
−2096
1118
−2361
−1203
789
−1796
499
6
−1823
−2416
1904
−1779
902
−818
786
−1249
−892
188



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


163(V
−884
−1453
−1331
−759
−1529
−2054
−754
426
1538
−1352
−623
−938
708
−503
609
−1024
716
1556
−1814
−1348
189



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


164(P)
−1205
−2096
−1371
−1294
−3530
1986
−1732
−3286
−1522
−3357
−2495
−1382
2600
1480
−1930
689
−1438
−2587
−3557
−3013
190



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


165(D
−1308
−2864
2377
1708
−3151
−1969
−847
−2932
908
−2865
−1984
1026
−2212
−422
−1139
−1136
−1274
−2473
−3039
1438
191



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


166(C
544
3117
−563
1527
−2011
−1598
−229
−1884
984
−1975
−920
−317
−1617
1132
−431
−599
−614
−1564
−1347
−1372
192



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


167(E)
−1135
−2652
805
2212
−2960
449
−701
−2724
−352
−2658
−1760
−534
−2098
1133
1725
−983
−1089
−2270
−2825
−2111
193



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


168(Y
−3596
−3426
−4011
−4236
−756
−3926
−2017
−3923
−4119
−3489
−3396
−3558
1274
−3712
−3935
−3640
−3783
−3834
−1423
4617
194



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−809
−7942
−1234
−894
−1115
−701
−1378
*
*


169(G
−2666
−2751
−3377
−3700
−4393
3753
−3528
−4841
−4031
−4741
−4286
−3394
−3411
−3824
−3848
−2912
−3053
−4080
−3719
−4290
195



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7148
−8190
−894
−1115
−2275
−334
*
*


170(H
−909
−2472
1648
1963
−2749
−1496
2279
−2536
−207
−2473
−1609
−112
−1774
−16
−789
911
−884
−2079
−2655
−1892
196



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7148
−8190
−894
−1115
−2275
−334
*
*


171(D
−1659
−3544
3062
1534
−3720
−1620
−946
−3633
−1158
−3508
−2820
1807
−2140
−608
−1992
−1327
−1718
−3114
−3702
−2701
197



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7148
−8190
−894
−1115
−2275
−334
*
*


172(G
−2666
−2751
−3377
−3700
−4393
3753
−3528
−4841
−4031
−4741
−4286
−3394
−3411
−3824
−3848
−2912
−3053
−4080
−3719
−4290
198



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−15
−7148
−8190
−894
−1115
−1358
−713
*
*


173(M
896
−558
−2670
−2060
1251
−2185
−1048
43
−1731
665
2262
−1717
−2232
−1411
−1654
−1259
1662
83
−1007
−665
199



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7414
−8456
−894
−1115
−1982
−421
*
*


174(L)
−3012
−2483
−4991
−4588
2563
−4681
−2567
−365
−4286
2641
286
−4144
−4140
−3283
−3862
−3990
−2889
−1078
−1633
−952
200



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7414
−8456
−894
−1115
−249
−2660
*
*


175(V
−1666
−1535
−3931
−3496
−1547
517
−2575
−46
−3176
1766
−593
−3023
−3348
−2859
−3069
−2394
−1705
2567
−2418
−2089
201



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


176(F)
−4576
−3559
−5015
−5343
4263
−4881
−1152
−3449
−4910
−2776
−2865
−3550
−4757
−3679
−4300
−4154
−4444
−3622
−401
2124
202



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


177(A
3345
−1674
−3727
−3977
−4175
−1941
−3439
−3955
−3910
−4243
−3329
−2621
−2737
−3468
−3709
825
−1527
−2788
−4405
−4282
203



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


178(W
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
204



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


179(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
205



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


180(C
−1236
5496
−4197
−4463
−4068
−2103
−3603
−3911
−4139
−4251
−3414
−2867
−2893
−3739
−3846
647
−1702
−2864
−4296
−4138
206



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


181(A
2681
−1648
−3686
−3867
−4184
2067
−3375
−3979
−3817
−4229
−3291
−2570
−2704
−3369
−3665
954
−1488
−2779
−4401
−4284
207



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


182(V
−2422
−1967
−4993
−4556
−1946
−4647
−4007
2482
−4386
447
1863
−4301
−4388
−4024
−4325
−3914
−2387
2602
−3399
−3128
208



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


183(N
−1642
−1630
−3011
−2702
−1675
−3027
−2261
−141
−2480
399
−827
3044
−3220
−2357
−2577
−2236
−1678
2152
−2448
−1987
209



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


184(I)
−1310
−1421
−2217
1185
−1569
−2673
−1609
2204
−1689
−1167
−660
−1843
2154
−1552
−1934
−1762
−1280
885
−2135
−1720
210



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


185(N
257
1558
440
612
−2481
−1765
−435
−2211
−31
−2194
1330
2360
−1863
1379
−534
−683
−729
−1796
−2393
−1727
211



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


186(P)
−2875
−3093
−3978
−4134
−3333
−3398
−3665
−3255
−3879
−167
−2945
−3727
4087
−3894
−3795
−3160
−3199
−3243
−3784
−3483
212



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


187(T)
−1617
−2991
2432
−518
−3614
−2086
−1314
−3413
−1230
−3383
−2559
996
−2497
−937
−1848
429
2498
−2889
−3583
−2811
213



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


188(A
2241
−1737
−1506
513
−2489
−1943
−1317
−2071
−1119
−2312
−1526
−1230
−2307
−1010
−1520
1714
−1101
416
−2692
−2195
214



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


189(E)
525
−2429
1776
1822
−2741
−1821
−551
−2497
−176
−2443
−1530
−453
124
1127
−700
−809
481
−2048
−2615
−1917
215



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


190(Q
−1567
−3175
1704
1474
−3449
−2060
−1054
−3249
−877
−3174
−2325
−671
−2377
2979
−1481
−1362
1084
−2781
−3353
−2563
216



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


191(L)
−3337
−2808
−5707
−5127
−1152
−5377
−4133
−460
−4878
2928
1801
−5079
−4636
−3831
−4452
−4675
−3207
697
−2886
−3022
217



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


192(A
3213
−1669
−3729
−3963
−4171
−1937
−3427
−3953
−3889
−4236
−3319
−2614
−2732
−3450
−3697
1430
−1520
−2784
−4399
−4276
218



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


193(E)
−2200
−4049
2514
2580
−4247
−2234
−1519
−4143
−1674
−4022
−3301
1158
−2729
−1171
−2473
318
−2248
−3627
−4213
−3243
219



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


194(I)
−2474
−2002
−5104
−4792
−2432
−4811
−4734
3681
−4697
−1199
−1182
−4556
−4627
−4560
−4788
−4186
−2477
1241
−4098
−3650
220



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


195(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
221



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


196(I)
−1091
−1076
−2583
413
−1145
−2551
−1453
2635
−1815
−911
−317
−1908
−2625
−1603
−1923
−64
352
1441
−1667
−1289
222



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


197(Q
518
−2071
−706
1236
−2329
−1787
−464
158
−79
−2066
−1190
−460
−1883
1523
−571
1071
1153
−1661
−2309
−1673
223



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


198(S)
690
−1632
−3665
−3707
−4013
−1911
−3224
−3755
−3549
−4023
−3114
−2519
−2683
−3185
−3470
2818
2263
−2676
−4240
−4079
224



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


199(A
2957
−1575
−3607
−3525
−3508
456
−2992
−3142
−3331
−3468
−2642
−2460
−2661
−2989
−3286
646
−1410
731
−3811
−3585
225



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


200(E)
56
−2745
1143
2154
−3045
−1930
−765
−2818
1875
−2752
−1859
945
−2144
−332
−1009
−1045
−1166
−2359
−2923
−2190
226



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


201(T)
−1256
−2015
−1982
−1726
−3420
−2098
−1785
−3082
927
−3191
−2379
−1663
−2604
−1463
−1433
524
3369
−2475
−3390
−2984
227



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


202(W
2769
−2207
−4068
−3807
2256
−3458
−1352
−1888
−3438
−1847
−1513
−2925
−3632
−2881
−3227
−2665
−2391
−1867
3112
296
228



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


203(R
−2470
−3357
−2623
1389
−4114
−3061
−1112
−3509
2409
−3196
−2485
−1713
−3049
−686
2844
−2333
−2214
−3234
−3079
−2887
229



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−540
−7942
−1697
−894
−1115
−701
−1378
*
*


204(M
816
−1388
−782
−226
−1511
−1668
−358
−1120
1679
119
1761
1028
−1755
−26
−458
−619
−511
−896
−1709
−1189
230



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7414
−8456
−894
−1115
−249
−2660
*
*


205(L)
−2837
−2376
−5287
−4745
−1320
−4877
−3831
1365
−4505
2429
1943
−4548
−4406
−3738
−4219
−4120
−2750
1537
−2900
−2889
231



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


206(C
−1191
2897
−3560
−2991
2519
−2720
−1807
−69
−2623
−837
−305
−2465
−2840
−2267
−2460
−1855
899
2022
−1675
−1297
232



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


207(G
−1539
−3140
2028
−312
−3421
2111
−1027
−3216
685
−3138
−2285
998
−2361
1202
−1409
−1339
−1520
−2749
−3312
−2532
233



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


208(I)
334
−1130
401
620
−1157
−2255
−1040
2289
−1141
−979
1812
−1358
−2323
−992
−1401
−1264
−866
644
−1591
−1181
234



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


209(E)
425
−2447
1685
2151
−2749
−1843
−586
−2497
831
−2456
−1553
−477
−1993
−142
−743
−847
−928
108
−2639
−1943
235



−149
−500
235
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−169
−3204
−8984
−206
−2913
−701
−1378
*
*


210(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
237



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


211(R
−2896
−3530
−3730
−2192
−4456
−3379
−1195
−3707
2481
−3319
−2662
−2040
−3291
1300
3145
−2762
−2535
−3495
−3134
−3075
238



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


212(V
−2838
−2616
−4834
−4880
−3213
−3955
−4416
−610
−4757
−2310
−2253
−4383
−4345
−4643
−4642
−3732
−3007
3763
−4157
−3886
239



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


213(A
3345
−1674
−3727
−3977
−4175
−1941
−3439
−3955
−3910
−4243
−3329
−2621
−2737
−3468
−3709
825
−1527
−2788
−4405
−4282
240



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


214(M
−3386
−2926
−5503
−5047
−1187
−5101
−3953
−644
−4586
916
4893
−4906
−4599
−3801
−4244
−4517
−3301
−1338
−2841
−2779
241



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


215(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4359
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
242



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


216(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
243



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


217(F)
−4596
−3564
−5022
−5356
4100
−4892
−1140
−3474
−4920
−2799
−2888
−3546
−4763
−3678
−4303
−4158
−4459
−3642
−389
2808
244



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


218(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
245



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


219(T)
−1086
−1700
−3489
−3680
−4071
−1960
−3290
−3842
−3622
−4127
−3243
−2550
−2741
−3278
−3511
1633
3501
−2755
−4299
−4122
246



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


220(K
−1211
−2188
−1252
−684
−2201
−2183
−735
−2041
2458
−2110
2342
1770
−2264
−372
−433
−1182
−1128
−1772
−2263
1110
247



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


221(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
248



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


222(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
249



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


223(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
250



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


224(K
−2345
−3215
−2680
−1702
−4004
−2994
−1114
−3430
3145
−3150
−2429
−1707
−3005
−690
1537
1210
−2130
−3134
−3052
−2847
251



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


225(H
−1041
−1842
−1795
−1535
−3225
1554
2940
−2958
−1555
−3088
−2233
−1512
−2445
−1442
−1925
2143
928
−2312
−3333
−2843
252



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


226(P)
−1812
−3493
1638
2272
−3746
−2139
−1234
−3572
714
−3480
−2668
−741
2320
−848
−1793
−1570
−1815
−3091
−3658
−2819
253



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


227(M
−1063
−1672
−1185
1731
−1647
−2144
−910
−1218
−725
1142
2726
1099
−2261
−651
−1101
−1179
−1008
−1100
−2011
−1532
254



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


228(W
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
255



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


229(V
−1683
−1779
−4225
−4114
−2704
−2952
−3573
−161
−3855
−1868
−1633
−3296
−3474
−3653
−3791
−2302
2073
3159
−3712
−3334
256



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


230(E)
576
−3124
2215
2294
−3448
−2061
−1092
−3240
−936
−3183
−2340
−696
−2393
−692
−1547
−1375
1093
−2772
−3374
−2589
257



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


231(K
315
−2794
−1360
−1079
−3606
−2397
−1201
−3242
3269
−3135
−2344
1290
−2657
−798
−553
−1655
−1723
−2800
−3186
−2733
258



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


232(V
−1806
−1830
−4356
−4254
−2729
−3152
−3789
−6
−4016
−1803
−1616
−3476
−3637
−3839
−3966
−2513
660
3495
−3859
−3459
259



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


233(Q
793
−1527
−1108
−552
−1618
−1947
1457
1157
637
−1440
−683
−772
−2030
1773
−839
−896
518
491
−1868
−1365
260



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


234(E)
300
−2398
695
1659
−2713
−1806
−518
−2470
1395
−2411
−1494
1531
−1936
1405
−648
−775
−852
−2018
−2579
−1883
261



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


235(A
3370
−1675
−3725
−3979
−4176
−1943
−3442
−3956
−3915
−4245
−3332
−2623
−2739
−3473
−3712
667
−1529
−2789
−4406
−4283
262



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


236(T)
−1487
−1747
−4009
−3951
−2829
−2596
−3358
−658
−3663
−2137
−1837
−3024
−3208
−3442
−3576
−1949
3247
2128
−3661
−3318
263



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


237(K
78
−2444
−636
1476
−2774
−1872
−554
−2516
2347
−2450
−1542
1061
−1996
1188
−567
−849
−918
−2073
−2605
−1935
264



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


238(L)
−2340
−2043
−4429
−3825
−1249
−4051
−2880
2209
47
2445
−154
−3576
−3849
−2947
−3012
−3227
−2264
−489
−2490
−2321
265



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


239(A
3066
−1532
−3416
−3204
−2546
−2126
−2613
−1745
−2919
251
−1678
−2434
−2743
−2680
−2903
−1429
721
−1481
−3078
−2775
266



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


240(K
−2775
−3478
−3431
−2065
−4356
−3299
−1170
−3648
3068
−3281
−2609
−1958
−3227
2481
1606
−2643
−2444
−3419
−3113
−3021
267



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


241(E)
5
−3248
−527
3274
−3691
−2238
−1242
−3429
736
−3351
−2559
−905
−2586
−855
−1282
−1630
−1816
−2982
−3491
−2801
268



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


242(R
1203
−1274
−1535
−960
714
−2112
−836
−874
1242
45
1502
−1087
−2184
−670
1607
−1097
−814
−732
−1655
−1220
269



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


243(A
1867
−2550
755
648
−2859
−1875
−646
−2618
−291
−2563
−1661
1632
−2046
−205
1386
−915
−1009
−2170
−2737
−2031
270



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


244(P)
−1833
−2267
−3769
−3944
−3591
−2645
−3527
−2530
−3805
−3285
−2936
−3132
3954
−3636
−3703
−2130
−2228
835
−4002
−3761
271



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


245(D
206
−2972
2710
1351
−3255
−2000
1755
−3042
−683
−2972
−2100
−614
−2269
1462
−1262
−1213
−1369
−2579
−3148
−2386
272



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


246(L)
−3414
−2874
−5769
−5161
1383
−5422
−4072
994
−4912
2728
2007
−5118
−4630
−3791
−4438
−4708
−3264
−1344
−2810
−2947
273



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


247(A
1831
−2029
575
−204
−2274
−1806
−494
−1962
927
−82
−1160
−494
270
−72
−607
−735
508
−1613
−2285
−1663
274



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


248(I)
−2431
−2000
−4947
−4466
1231
−4532
−3688
3189
−4249
517
−529
−4183
−4266
−3781
−4114
−3766
−2383
1363
−3060
−2816
275



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


249(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
276



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


250(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
277



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


251(E)
−4205
−4417
−2343
3901
−5349
−3698
−3534
−5837
−3838
−5561
−5237
−3039
−4218
−3453
−4201
−4072
−4352
−5409
−4640
−4860
278



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


252(L)
−3602
−3015
−5919
−5328
1158
−5654
−4138
−616
−5093
2938
2013
−5330
−4744
−3877
−4575
−4984
−3438
−1455
−2794
−2838
279



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


253(Q
−4207
−4117
−3531
−3731
−4752
−3930
−3721
−5554
−3395
−5179
−4897
−3823
−4399
4556
−3405
−4301
−4380
−5208
−4301
−4384
280



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


254(F)
−2863
−2433
−5172
−4640
3169
−4702
−3234
−392
−4348
1785
−84
−4307
−4290
−3544
−4021
−3916
−2769
1504
−2393
−2005
281



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


255(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
282



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


256(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
283



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


257(A
3186
−1669
−3728
−3961
−4171
−1936
−3425
−3953
−3886
−4235
−3318
−2613
−2731
−3447
−3695
1524
−1520
−2783
−4398
−4275
284



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


258(F)
−3002
−2525
−5316
−4818
3177
−4924
−3350
2613
−4551
818
−147
−4496
−4441
−3703
−4215
−4169
−2905
−737
−2444
−1989
285



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


259(V
−2294
−2438
724
−2485
−3071
−3156
−3064
−720
−3372
−2390
−2164
−2598
−3629
−2975
−3716
−2757
−2460
3538
−3861
−3370
286



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


260(P)
−1594
−2934
−619
588
−3418
−2163
−1118
−3148
755
−3092
−2272
−870
3320
−720
−1108
321
−1590
−2706
−3251
−2595
287



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


261(E)
232
−2432
−633
1887
−2799
−1880
−622
−2541
1768
−2491
−1591
−543
−2032
−178
−679
1460
−962
−2096
−2660
−1989
288



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


262(V
−2838
−2616
−4834
−4880
−3213
−3955
−4416
−610
−4757
−2310
−2253
−4383
−4345
−4643
−4642
−3732
−3007
3763
−4157
−3886
289



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


263(A
3303
−1827
−3692
−3978
−4328
1129
−3541
−4137
−4037
−4403
−3503
−2739
−2867
−3583
−3840
−1477
−1693
−2962
−4468
−4428
290



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


264(E)
1471
−2341
669
1713
−2646
−1806
−509
−2389
1384
−680
−1440
−444
−1930
−60
−624
−766
−833
−1955
−2531
−1848
291



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


265(Q
236
−2063
−853
−302
−2314
−1882
−542
−1988
1473
779
−1199
−578
−1977
1845
−534
1012
−820
−1652
−2307
−1712
292



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


266(K
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
293



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


267(A
3348
−2109
−3336
−3412
−1764
−2509
−2437
−2956
−3286
−3118
−2630
−2708
−3150
−3050
−3249
−1903
−2016
−2533
−2364
1487
294



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


268(P)
−1528
−2315
−1720
−1687
−3833
−2233
−1895
−3559
797
−3582
−2793
−1690
3653
−1574
−1445
406
−1768
−2881
−3673
−3270
295



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


269(G
−1935
−3089
2442
−885
−4418
2762
−1974
−4330
−2285
−4310
−3586
−1182
−2821
−1681
−3048
394
−2183
−3549
−4481
−3646
296



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


270(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
297



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


271(K
−986
−2487
579
781
−2798
−1840
−584
−2559
1709
−2499
−1588
1699
1485
−137
−743
394
−935
−2105
−2668
−1964
298



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


272(V
−2488
−1997
−5115
−4741
−2236
−4876
−4521
1399
−4638
586
−992
−4534
−4596
−4394
−4675
−4204
−2466
3282
−3849
−3500
299



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


273(A
2860
−2613
−1445
−1105
−3345
−2365
−1181
−2968
911
−2930
−2150
−1269
−2615
1502
−562
−1587
−1627
−2570
−3048
−2602
300



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


274(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
301



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


275(H
−1098
−2519
644
−333
−2880
−1987
2767
−2597
2281
−2509
−1617
857
−2093
−153
1114
−970
−1024
−2167
−2636
−2015
302



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


276(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
303



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


277(N
−1586
−2305
−1738
−2020
−4028
−2232
−2587
−3973
−2635
−4158
−3406
4054
−2920
−2402
−2900
−1720
681
−3116
−4157
−3676
304



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


278(V
−2446
−1963
−5100
−4786
−2548
−4847
−4816
1583
−4714
−1330
−1276
−4562
−4651
−4631
−4841
−4218
−2447
3468
−4224
−3729
305



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


279(W
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
306



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


280(F)
−3486
−2932
−5765
−5195
3114
−5447
−3820
−632
−4937
2173
2384
−5073
−4656
−3798
−4452
−4740
−3334
−1436
−2628
−2474
307



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


281(I)
−2458
−1961
−5125
−4807
−2569
−4914
−4890
3219
−4751
−1339
−1284
−4603
−4685
−4676
−4892
−4286
−2453
2471
−4276
−3778
308



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


282(F)
−4720
−3927
−5139
−5454
4513
−4504
−2520
−3938
−5326
−3307
−3429
−4469
−4782
−4575
−4818
−4787
−4810
−4172
−1812
−736
309



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


283(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
310



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


284(D
−2322
−4185
3035
1469
−4418
−2266
−1625
−4341
−1869
−4214
−3539
−867
−2800
−1293
−2724
1786
−2396
−3805
−4409
−3395
311



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


285(L)
−3492
−2925
−5831
−5302
−1190
−5571
−4334
974
−5050
3059
17
−5312
−4763
−3965
−4606
−4957
−3364
−1095
−2972
−3089
312



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


286(Q
−2004
−3769
1173
2079
−3991
−2187
−1375
−3849
−1403
−3743
−2969
1136
−2626
3177
−2117
−1727
−2027
−3352
−3926
−3026
313



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


287(A
2861
2401
−3941
−3933
−3713
−1915
−3209
−3405
−3647
−3703
−2846
−2573
−2679
−3249
−3498
1246
795
−2497
−4004
−3830
314



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


288(G
804
−1917
−3555
−3872
−4406
3488
−3564
−4245
−4053
−4497
−3611
−2768
−2934
−3603
−3880
−1567
−1787
−3064
−4502
−4481
315



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


289(N
−3642
−3838
−3004
−3356
−4743
−3612
−3773
−5643
−4099
−5460
−5060
4378
−4185
−3865
−4225
−3744
−3950
−5002
−4391
−4381
316



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


290(I)
−3202
−2787
−5004
−4996
−2836
−4384
−4440
3937
−4838
−1815
−1871
−4704
−4600
−4683
−4704
−4353
−3280
−603
−3938
−3661
317



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


291(G
1452
−1874
−3596
−3903
−4376
3335
−3550
−4203
−4044
−4459
−3565
−2747
−2900
−3588
−3862
−1522
−1740
−3018
−4492
−4461
318



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


292(Y
−4791
−3935
−4914
−5231
−815
−4530
−2178
−4357
−5024
−3745
−3807
−4206
−4772
−4328
−4584
−4677
−4837
−4435
−1477
4857
319



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


293(K
−4125
−4091
−4017
−3615
−5101
−3960
−2958
−5182
3972
−4825
−4345
−3552
−4249
−2709
−1809
−4154
−4044
−4895
−4132
−4389
320



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


294(I)
−3130
−2603
−5572
−5096
−1392
−5301
−4317
3061
−4881
2084
−185
−5010
−4688
−4034
−4578
−4656
−3046
−463
−3127
−3143
321



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


295(A
2675
−1535
−3836
−3683
−2774
−2214
−2959
−1215
−3410
−2393
−1857
−2670
−2859
−3085
−3310
−1533
792
2057
−3350
−3057
322



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


296(Q
−4207
−4117
−3531
−3731
−4752
−3930
−3721
−5554
−3395
−5179
−4897
−3823
−4399
4556
−3405
−4301
−4380
−5208
−4301
−4384
323



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


297(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
324



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


298(F)
−3500
−2941
−5773
−5206
3166
−5461
−3812
−635
−4949
2227
2003
−5083
−4665
−3805
−4462
−4757
−3346
−1441
−2619
−2449
325



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


299(G
2279
−1650
−3675
−3870
−4196
2606
−3385
−3993
−3836
−4243
−3303
−2572
−2706
−3382
−3678
828
−1491
−2786
−4412
−4298
326



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


300(K
−1571
−2888
−927
−655
−3321
2063
1468
−3019
2355
−2910
−2082
920
−2447
−523
−551
−1431
−1504
−2612
−2998
−2431
327



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


301(F)
2448
−2615
−4303
−4172
2956
−3922
−1214
−2324
−3828
−2183
−1926
−3107
−3996
−3105
−3543
−3119
−2926
−2325
−560
2239
328



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


302(E)
−1402
−2920
1061
2832
−3242
−2054
−879
−2998
774
−2910
−2042
−672
−2284
−454
1073
−1230
−1357
−2549
−3055
−2355
329



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


303(A
3609
−2508
−4184
−4493
−4592
−2737
−3989
−4421
−4496
−4701
−4051
−3440
−3474
−4171
−4253
−2294
−2487
−3536
−4484
−4643
330



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


304(I)
−1878
−1552
−4312
−3775
−1491
−3734
−2739
2447
−3471
521
−541
−3367
−3656
−3110
−3335
−2889
−1832
2126
−2430
2171
331



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


305(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
332



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


306(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
333



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


307(I)
−2574
−2082
−5179
−4778
−2009
−4923
−4424
3104
−4656
1327
−776
−4584
−4587
−4278
−4620
−4242
−2542
1595
−3640
−3399
334



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


308(C
−1490
2918
−3842
−3258
−1170
−3142
−2133
1934
−2902
1595
−258
−2806
−3152
−2528
−2741
−2266
1394
65
−1929
−1611
335



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


309(Q
−4207
−4117
−3531
−3731
−4752
−3930
−3721
−5554
−3395
−5179
−4897
−3823
−4399
4556
−3405
−4301
−4380
−5208
−4301
−4384
336



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


310(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
337



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


311(L)
−3686
−3084
−5727
−5319
2036
−5453
−3331
−789
−5052
2929
−88
−4966
−4743
−3883
−4542
−4837
−3542
−1589
−2266
−1680
338



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


312(A
2898
−1987
−2240
−2485
−4049
−2085
−2826
−3885
−3004
−4117
−3288
2707
−2813
−2683
−3186
−1506
−1726
−2917
−4217
−3861
339



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


313(K
1066
−2626
−1795
−1052
−3050
−2498
−872
−2618
2866
−2563
1526
−1206
−2543
−458
1142
−1556
−1494
−2321
−2682
−2281
340



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


314(P)
−4497
−4117
−4899
−5251
−5560
−4139
−4798
−6328
−5454
−5976
−5741
−5026
4302
−5328
−5104
−4798
−4844
−5739
−4665
−5484
341



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


315(V
−2475
−1980
−5117
−4752
−2317
−4893
−4598
2349
−4663
557
−1062
−4548
−4617
−4458
−4725
−4228
−2455
2932
−3943
−3564
342



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


316(H
173
−2262
−944
−767
−3091
−1973
2974
−2865
−947
−2905
−2052
2949
−2338
−840
−1414
1020
−1292
−2346
−3104
−2474
343



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


317(D
−4232
−4465
4158
−2649
−5412
−3676
−3575
−6036
−4125
−5722
−5419
−2993
−4216
−3512
−4617
−4077
−4401
−5553
−4696
−4914
344



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


318(L)
−4119
−3542
−5389
−5357
−2027
−4767
−4359
−1609
−5118
3293
−979
−5230
−4771
−4474
−4724
−5069
−4106
−2331
−3412
−3400
345



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


319(S)
−1944
−2458
−3696
−4011
−4410
−2644
−3753
−4675
−4191
−4807
−4070
−3198
−3384
−3886
−4051
3656
−2393
−3615
−4374
−4307
346



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


320(R
−4488
−4181
−4789
−4318
−5193
−4148
−3436
−5568
−2393
−5152
−4748
−4156
−4479
−3286
4202
−4614
−4467
−5273
−4267
−4649
347



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


321(G
−4088
−3924
−4774
−5139
−5615
3825
−4753
−6303
−5453
−6014
−5662
−4812
−4539
−5232
−5106
−4370
−4472
−5527
−4696
−5561
348



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


322(C
−1383
5545
−4327
−4558
−4019
−2243
−3671
−3414
−4157
−3975
−3301
−3006
−3016
−3818
−3871
−1656
540
−2675
−4269
−4170
349



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


323(S)
−1998
−3389
920
−646
−4194
−2202
−1688
−4095
−1857
−4037
−3301
1873
−2745
−1360
−2596
2972
−2145
−3473
−4215
−3333
350



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


324(A
1796
−2218
759
1121
−2509
−1874
−667
−2198
−343
−2259
−1405
−578
−2039
−254
−849
333
−937
1127
−2521
−1880
351



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


325(E)
−2264
−4130
1161
3061
−4316
−2257
−1556
−4221
−1720
−4092
−3390
−846
−2763
2340
−2512
−1940
−2316
−3705
−4277
−3299
352



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


326(D
−33
−4335
3607
1205
−4570
−2294
−1708
−4511
−2035
−4382
−3756
−897
−2858
−1390
−2952
−2084
−2528
−3963
−4583
−3525
353



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


327(I)
−2456
−1959
−5125
−4808
−2580
−4919
−4900
3012
−4754
−1350
−1292
−4605
−4688
−4684
−4898
−4291
−2452
2731
−4288
−3786
354



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


328(Y
−2065
−1703
−4458
−4000
−1497
−3978
−2824
1512
−3720
−1096
−877
−3567
−3903
−3404
−3627
−3169
−2033
2744
−2424
2949
355



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



9—
−7942
−8984
−894
−1115
−701
−1378
*
*


329(K
−1420
−3005
1129
1097
−3290
1087
−935
−3080
2093
−3005
−2135
1724
−2283
−517
−1280
−1233
−1393
−2613
−3178
−2412
356



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


330(W
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
357



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


331(V
242
−1739
−4505
−4069
−1901
−3865
−3328
339
−3811
1145
−812
−3662
−3892
−3515
−3741
−3100
−2045
3053
−3031
−2693
358



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


332(A
2785
−1548
−3757
−3706
−3183
−2010
−3016
−2274
−3464
−3018
−2323
−2559
−2725
−3105
−3346
519
−1436
1956
−3604
−3353
359



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


333(A
2301
−1785
−4329
−3814
−1389
−3715
−2881
1608
−3501
1601
−328
−3440
−3685
−3079
−3364
−2901
−2013
−142
−2516
−2281
360



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


334(I)
−2461
−1969
−5121
−4803
−2539
−4894
−4855
3457
−4740
−1307
−1260
−4593
−4673
−4650
−4870
−4267
−2458
2020
−4235
−3750
361



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


335(T)
1694
−1679
−3770
−3955
−4023
−1971
−3381
−3579
−3783
−3994
−3161
−2633
−2754
−3407
−3608
−1339
3474
−2625
−4286
−4150
362



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


336(A
3145
2363
−4124
−4092
−3068
−2102
−3176
−1748
−3745
−2761
−2179
−2727
−2812
−3341
−3530
−1439
−1489
622
−3594
−3345
363



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


337(V
1549
−1561
−3980
−3800
−2617
−2450
−3066
−638
−3529
−2084
−1647
−2851
−3038
−3217
−3427
−1764
645
3033
−3323
−3003
364



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


338(Q
−1998
−3480
−470
1001
−3872
−2292
−1373
−3668
−1085
−3566
−2804
−947
1060
3783
−1533
−1779
−2007
−3214
−3690
−2964
365



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


339(A
2845
−1741
−2441
−2116
−3476
181
−2133
−3194
−1899
−3346
−2475
−1852
−2527
−1850
1122
752
−1333
−2425
−3579
−3191
366



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−9
−7942
−8984
−894
−1115
−701
−1378
*
*


340(Q
288
−1371
−2820
−2256
−1089
−2819
−1668
1024
−1933
1596
−165
−2151
−2848
2772
−2022
−1891
−1329
−469
−1817
−1496
367



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−444
−7942
−1940
−894
−1115
−701
−1378
*
*


341(A
2553
−1499
−1618
−1106
−1437
−1994
−884
−1437
−516
−1637
−947
−1150
−2220
−717
1070
−1116
−989
−1219
−1772
1598
368



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−154
−7510
−3381
−894
−1115
−1844
−471
*
*


342(Q
−840
−2183
2051
46
−2487
478
−471
−2205
−189
−2230
−1379
−273
−1821
2234
−719
−721
−813
565
−2466
−1782
369



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−13
−7368
−8411
−894
−1115
−2041
−402
*
*


343(K
−542
−1518
903
−68
−1686
266
−294
542
1621
−1463
−666
−332
−1689
78
−434
−539
−484
817
−1827
−1263
370



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−435
−7368
−1975
−894
−1115
−2041
−402
*
*


344(K
842
−1833
1221
299
−2188
−1329
−107
−1924
1349
−1907
−1015
−8
−1492
329
−247
1153
−413
−1495
−2105
−1431
371



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−1110
−6951
−919
−894
−1115
−2432
−296
*
*


345(R
−956
−1423
−1186
−681
−1830
−1454
−276
−1686
690
−1640
−1095
−694
−1696
14
3253
−1003
−929
−1464
−1574
−1319
372



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


346(E)
−804
−1735
497
2874
−2096
−1058
−327
−1858
−157
−1967
−1380
40
−1471
−39
−568
−688
−853
−1562
−2027
−1547
373



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


347(R
−956
−1423
−1186
−681
−1830
−1454
−276
−1686
690
−1640
−1095
−694
−1696
14
3253
−1003
−929
−1464
−1574
−1319
374



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


348(S)
227
−390
−660
−668
−1564
−565
−762
−1409
−705
−1690
−1020
−460
−1182
−625
−900
2604
−94
−861
−1818
−1338
375



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


349(L)
−1162
−1035
−2273
−2015
−35
−2243
−1374
599
−1587
2466
801
−1903
−2350
−1486
−1587
−1745
−1181
283
−1141
−718
376



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


350(H
−948
−1353
−590
−533
−353
−1374
4354
−1609
−210
−1544
−1090
−628
−1716
−415
−369
−985
−1004
−1409
−758
74
377



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


351(T)
71
−435
−987
−905
−1386
−761
−851
−654
−738
−1152
−667
−673
−1327
−733
−889
−159
2865
−370
−1730
−1351
378



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


352(L)
−1162
−1035
−2273
−2015
−35
−2243
−1374
599
−1587
2466
801
−1903
−2350
−1486
−1587
−1745
−1181
283
−1141
−718
379



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


353(V
−558
−557
−2112
−1895
−730
−1799
−1509
1150
−1619
15
149
−1639
−2121
−1558
−1693
−1183
−681
2778
−1647
−1187
380



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−2888
−209
*
*


354(E)
−804
−1735
497
2874
−2096
−1058
−327
−1858
−157
−1967
−1380
40
−1471
−39
−568
−688
−853
−1562
−2027
−1547
381



−149
−500
233
43
−381
399
106
−626
210
−466
−720
275
394
45
96
359
117
−369
−294
−249



−37
−5878
−6920
−894
−1115
−127
−3565
*
*


355(W
−150
757
49
299
−277
−651
839
−485
392
−910
599
300
−401
623
162
−99
38
−448
1107
126
382



*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*



*
*
*
*
*
*
*
*
0








Claims
  • 1-4. (canceled)
  • 5. A recombinant host cell comprising: (i) at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity; and(ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
  • 6. The recombinant host cell of claim 5, wherein said host cell further comprises: (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
  • 7. The recombinant host cell of claim 5, wherein said host cell has a reduced requirement for an exogenous two-carbon substrate for its growth in culture compared to a recombinant eukaryotic host cell comprising (i) and not (ii).
  • 8. The recombinant host cell of claim 5, wherein said host cell has improved growth as compared to a host cell comprising (i) and not (ii) in culture media that is not supplemented with an exogenous two-carbon carbon substrate.
  • 9. The recombinant host cell of claim 8, wherein said exogenous to-carbon substrate is ethanol or acetate.
  • 10. (canceled)
  • 11. The recombinant host cell of claim 5, wherein said endogenous gene encoding a polypeptide having pyruvate decarboxylase activity is PDC1, PDC5, PDC6, or combinations thereof and said host cell is S. cerevisiae.
  • 12. The recombinant host cell of claim 5, wherein said heterologous polynucleotide encoding a polypeptide having phosphoketolase activity is xpk1 from Lactobacillus plantarum, xpkA from Lactobacillus pentosus MD363 or 6-phosphate phosphoketolase from B. lactis.
  • 13. The recombinant host cell of claim 5 wherein said polypeptide having phosphoketolase activity comprises at least 85% identity to SEQ ID NO 481 or an active fragment thereof.
  • 14. The recombinant host cell of claim 6, wherein said heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity is EutD from Lactobacillus plantarum or phosphotransacetylase from Bacillus subtilis.
  • 15. The recombinant host cell of claim 6, wherein said, polypeptide having phosphotransacetylase activity comprises at least 85% identity to SEQ ID NO 1472 or an active fragment thereof.
  • 16. The recombinant host cell of claim 5, wherein said host cell is a member of the genera Clostridium, Zymomonas, Escherichia, Salmonella, Senatia, Erwinia, Klebsiella, Shigella, Rhodococcus, Pseudornonas, Bacillus, Lactobacillus, Enterococcus, Alcaligenes, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Schizosaccharomyces, Kluyveromyces, Yarrowia, Pichia, Candida, Hansenula, or Saccharomyces.
  • 17. The recombinant host cell of claim 5 wherein said host, cell is Saccharomyces cerevisiae.
  • 18. The recombinant host cell of claim 5 further comprising a pyruvate-utilizing biosynthetic pathway, wherein said pyruvate-utilizing biosynthetic pathway forms a product selected from the group consisting of: 2,3-butanediol, 2-butanol, 2-butanone, valine, leucine, lactic acid, malate, and isoamyl alcohol.
  • 19. The recombinant host cell of claim 5, further comprising a pyruvate-utilizing biosynthetic pathway herein said pyruvate-utilizing biosynthetic pathway is an isobutanol biosynthetic pathway comprising the substrate to product conversions: (i) pyruvate to acetolactate;(ii) acetolactate to 2,3-dihydroxyisovalerate;(iii) 2,3-dihydroxyisovalerate to 2-ketoisovalerate;(iv) 2-ketoisovalerate to isobutyraldehyde;(v) isobutyraldehyde to isobutanol; and
  • 20. The recombinant host cell of claim 18, wherein said pyruvate-utilizing biosynthetic pathway is a 2-butanone biosynthetic pathway comprising the substrate to product conversions: (i) pyruvate to acetolactate;(ii) acetolactate to acetoin;(iii) acetoin to 2,3-butanediol;(iv) 2,3-butanediol to 2-butanone;
  • 21. The recombinant host cell of claim 18, wherein said pyruvate-utilizing biosynthetic pathway is a 2-butanol biosynthetic pathway comprising the substrate to product conversions: (i) pyruvate to acetolactate;(ii) acetolactate to acetoin;(iii) acetoin to 2,3-butanediol;(iv) 2,3-butanediol to 2-butanone;(v) 2-butanone to 2-butanol;
  • 22. A method for the production of 2,3-butanediol, 2-butanol, 2-butanone, valine, leucine, lactic acid, malic acid, alanine, fumaric acid, succinic acid or isoamyl alcohol comprising growing the recombinant host cell of claim 18 under conditions wherein the product is produced and optionally recovering the product.
  • 23. A method for the production of isobutanol comprising growing the recombinant host cell of claim 19 under conditions wherein the product is produced and optionally recovering the product.
  • 24-27. (canceled)
  • 28. A method of improving the growth of a recombinant host cell comprising at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity, comprising: transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity,
  • 29. The meth d of claim 28, wherein said method further comprises: transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
  • 30. The method of claim 28, further comprising growing the recombinant host cell in media containing limited carbon substrate.
  • 31. A method of reducing the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least genetic modification reduces or eliminates pyruvate decarboxylase activity, comprising: transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity, wherein the recombinant host cell comprising the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity has a reduced requirement for an exogenous two-carbon substrate as compared to a control recombinant host without the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
  • 32. The method of claim 31, wherein said method further comprises: transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
  • 33. The recombinant host cell of claim 5 wherein the phosphoketolase matches the Profile HMM given in Table 6 with an E value of less than 7.5E-242.
  • 34. The recombinant host cell of claim 5 wherein the phosphoketolase matches the Profile HMMs given in Tables 6, 7, 8, and 9 with E values of less than 7.5E-242, 1.1E-124, 2.1E-49, 7.8E-37, respectively.
  • 35. The recombinant host cell of claim 5 further comprising a phosphotransacetylase which matches the Profile HMM given in Table 14 with an E value of less than 5E-34.
  • 36. The recombinant host cell of claim 18, wherein said pyruvate-utilizing biosynthetic pathway is a 2,3-butanediol biosynthetic pathway comprising the substrate to product conversations: (i) pyruvate to acetolactate;(ii) acetolactate to acetoin;(iii) acetoin 2,3-butanediol; and
CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of and claims the benefit of U.S. application Ser. No. 13/161,168, filed on Jun. 15, 2011, which is related to and claims the benefit of priority of U.S. Provisional Patent Application No. 61/356,379, filed on Jun. 18, 2010. Each of the referenced applications is herein incorporated by reference.

Provisional Applications (1)
Number Date Country
61356379 Jun 2010 US
Continuations (1)
Number Date Country
Parent 13161168 Jun 2011 US
Child 14335734 US