Recombinase Genome Editing

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Feb. 2, 2017, is named 010498_00906-WO_SL.txt and is 161,995 bytes in size.

FIELD

The present invention relates in general to genome editing methods that use foreign recombinases.

BACKGROUND

Oligonucleotide-mediated recombination is used for genome engineering (see Carr and Church 2009) where mutation-encoding oligonucleotides modify the genome without the need for site-specific DNA-binding proteins. Such techniques may be used to generate large complex libraries of genetic variants. Such techniques may also be used to generate complex, user-defined genotypes at high efficiency in E. coli. (see Wang et al., 2009). Oligo-mediated recombination has enabled multiple synthetic biology applications such as genetically recoded organisms (Lajoie et al., 2013) and sensor-based metabolic pathway optimization (Raman et al., 2014).

Efforts at recombineering (i.e., recombination-mediated genetic engineering) (are present in (Van Pijkeren and Britton, 2012), (van Pijkeren et al., 2012), (van Kessel and Hatfull, 2007), (Binder et al., 2013), (Datta et al., 2008). In E. coli, oligo-mediated targeting is most commonly done via λ Red recombineering, where an oligo preferentially anneals to the lagging strand of the genome during DNA replication and incorporates into the daughter strand (Ellis et al., 2001a). This system is based on the phage λ Red operon normally expressed during the phage's lytic growth (Poteete, 2001) and promotes high-efficiency, targeted recombination between linear, single-stranded (Mosberg et al., 2010) DNA (ssDNA) and the host chromosome. The λ Red operon is composed of Red α, β and γ, also known as exo (a 5′→3′ exonuclease), beta (a single stranded annealing protein [SSAP]), and gam (a RecBCD nuclease complex inhibitor), respectively. λ β is necessary and sufficient to recombine ssDNA into the E. coli chromosome and itself improves recombination rates in by 1E4-fold (Ellis et al., 2001b). The β-mediated recombination is based on the input ssDNA both directing proper targeting and encoding mutations of interest.

SUMMARY

The disclosure provides methods of optimizing genome editing in organisms, such as bacteria. The disclosure provides for the identification of recombinases that can be used for genome editing in organisms, such as bacteria. A recombinase may also be referred to herein as a single strand annealing protein. Genome editing includes the use of a recombinase to recombine genomic DNA to include a donor nucleic acid sequence such as a single stranded DNA (ssDNA). Such genome editing may be known in the art as “recombineering.” The disclosure provides for the identification and use of components sufficient to produce introduction of a foreign nucleic acid sequence into the genome of a cell. One or more or all of such components may be foreign to the cell. Such components include a recombinase (also referred to as a single strand annealing protein or SSAP) and a single-strand binding protein. The disclosure provides for the identification of one or more pairs of a recombinase and a single-stranded binding protein that can be used in genome editing to incorporate an ssDNA into a genome. A single stranded binding protein (SSB) or a single stranded annealing protein (SSAP) is one that participates in replication, repair or recombination. An exemplary recombinase used for recombineering is λ Red as described in (Carr et al., 2012; Lajoie et al., 2012; Miki et al., 2008; Mosberg et al., 2012; Wang et al., 2009, 2011). An exemplary single-stranded binding protein is single-strand DNA-binding protein (SSB), an example of which is found in E. coli. See Meyer R R, Laine P S (December 1990), Microbiol. Rev. 54 (4): 342-80. Other exemplary recombinases or single-strand DNA-binding proteins may be found in other bacteria and viruses. The disclosure provides that either one or both of a recombinase and a corresponding single-stranded binding protein is foreign to the organism which uses them for genome editing or into which they are provided. According to one aspect, the recombinase and a corresponding single-stranded binding protein are provided to a cell as native species or as a nucleic acid encoding the recombinase or the corresponding single-stranded binding protein for expression within the cell. The disclosure provides a method of genome editing by including one or more or both of a recombinase and a corresponding single-stranded DNA-binding protein into a cell where one or more or both of a recombinase and a corresponding single-stranded DNA binding protein is foreign to the cell and where a donor nucleic acid sequence is introduced into the genome of the cell. The disclosure provides that the combination of a recombinase and a corresponding single-stranded DNA binding protein provide the minimal functional units used by a cell to insert ssDNA into its genome. The recombinase and a corresponding single-stranded DNA binding protein may be evolved from the same or different organisms. However, at least one is foreign to the cell into which they are provided or are otherwise present.

The disclosure provides a library-based method of identifying candidate single-stranded annealing proteins for use in oligo-recombination. The disclosure provides a library-based method of identifying candidate single-stranded annealing proteins from various and diverse organisms for use in oligo-recombination.

The disclosure provides a method by which β anneals complementary ssDNA pre-coated with SSB which is dependent on the C-terminal 8 amino acid tail of SSB. The disclosure provides a method by which the C-terminus of λ β is involved in its interaction with SSB. The disclosure provides a method of co-expressing a low-activity SSAP and its corresponding SSB to achieve oligo recombination. The λ β-SSB is a minimal functional unit of recombination and constitutes a host interaction node regulating recombination frequencies.

Further features and advantages of certain embodiments of the present invention will become more fully apparent in the following description of embodiments and drawings thereof, and from the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee. The foregoing and other features and advantages of the present embodiments will be more fully understood from the following detailed description of illustrative embodiments taken in conjunction with the accompanying drawings in which:

FIG. 1 depicts in schematic a Serial Evolutionary Enrichment for Recombinases (SEER) workflow. A complex plasmid library bearing the synthetic recombinases to be tested was transformed into a reporter E. coli strain containing two chromosomally integrated broken antibiotic resistance markers, tolC and cat. These broken resistance markers can then be repaired by two serial oligo recombination and selection, effectively enriching for cells with functional recombinases. After this the selected plasmids were extracted and re-transformed into the original strain, completing two Rounds of Enrichment (RoE). After six RoE we directly PCR′d the barcode region from the recombinase insert with Illumina-compatible primers to prepare indexed libraries for deep sequencing analysis.

FIG. 2A-2C depict results of recombinase discovery using SEER in E. coli. Using the first library (72+10 recombinases), multiple library configurations including Library 1.1 (72 recombinases of unknown function), Library 1.2 (1.1+9 recombinases known to function to varying degrees in E. coli), and Library 1.3 (1.2+λ β) were prepared. SEER was conducted with these 3 library configurations. FIG. 2A: After 6 RoE, the frequencies of enriched SSAPs were quantified by Sanger sequencing of their corresponding barcodes from 48 clones. FIG. 2B: The Allele Recombination (AR) Frequency of the most abundant SSAPs were characterized from (A) by quantifying oligo recombination frequencies of fixing a tolC marker (using tolC-Lnull_revert) via serial dilution and colony counting. The data are presented as mean±Std. Error of the Mean with the scatter plotted and color coded to match (A). These data are the result of at least 4 biological replicates. FIG. 2C: Toxicity of SSAPs from (A) and (B) were tested using kinetic growth assays with (+L-Ara) and without L-arabinose (−L-Ara) induction. Two negative controls, pARC8.GFP and empty pARC8 were used. Growth kinetics were distilled into doubling time as calculated previously {24136967}. These data are the result of 3 biological replicates and are color coded to match (A) and (B).

FIG. 3A-3B depict Expanded Recombinase Search Space Using SEER in E. coli. In order to better sample the bacteriophage derived SSAP sequence space, a Hidden Markov Model-based search strategy using multiple known recombinases was used to generate the position matrix with which to search nucleotide databases. This new searched contributed 113 new SSAP, for a total recombinases library size of 195 members. FIG. 3A: Phylogenetic relationship between Single-strand annealing protein library members. The 6 clades of phage-derived SSAPs are color coded: redβ (red); sak (yellow); erf (light blue); gp2.5 (light green); sak4 (purple); and uvsX (orange). FIG. 3B: The population of recombinases was sequenced at each step of SEER, included before any enrichment (0 RoE). The population distribution of unique members was plotted as a stack plot with RoE on the x-axis. Over subsequent RoE, the population diversity of this SEER linage drops as the system converges on a solution.

FIG. 4A-4D depict characterizing the C-terminal of Beta. FIG. 4A: In order to test the λ β mediated interactions between SSAPs and SSB, the C-terminus of λ β protein was serially truncated into various fragments (177, 194, 211, 228, 245 amino acids, where 266 is wildtype). These variants were expressed on pARC8 and transformed into the SEER chassis to measure GFP reversion using GFP.r2_revert, followed by flow cytometric analysis of the GFP+ population (reported as % AR). FIG. 4B: Same as in (A), but using single alanine substitutions in λ β. FIGS. 3C and 3 D: Gel shift assay showing the ssDNA binding of a subset of beta mutations: wild type (red square); truncation mutant β1-194 (orange triangle); point mutants βK214A (blue circle); βK172A (green diamond). In addition the recombinase Q8 was included (purple square).

FIG. 5A-5C depict that beta interacts with SSB in a Mg²⁺-dependent reaction. FIG. 5A: Fluorescent oligo quenching assay. Briefly, two complementary oligos with compatible FITC fluorophore and quencher anneal and lead to a decay in the fluorescence intensity that can be tracked over time (1). Thus, fluorescence intensity will be proportional to the amount of starting substrate, while the remaining fraction will be the annealed product. If the oligos are coated with SSB prior mixing, they will be prevented from annealing unless additional factors are able to remove the inhibition (2). The following traces are representative examples of an experiment that was carried out at least 4 times. FIG. 5B: Two SSB-coated complementary oligos were incubated with λ (3 in the presence of 10 mM MgCl₂(red curve) and 1 mM EDTA (blue curve) showing that λ β is capable of removing the annealing inhibition of SSB in a Mg′-dependent reaction. In addition, the assay was repeated with SSBdC8 coated oligos (green curve), which abolished the de-inhibitory activity λ β. This suggests that like in other SSB-interacting proteins, the C-terminal 8-amino acid tail of SSB is required for its interaction with λ β. FIG. 5C: Annealing of SSB-coated oligos in the presence of various SSAPs. Only full length λ β WT is capable of annealing SSB-coated oligos (red curve), while the 1-194 truncation is indistinguishable from the no SSAP control (orange vs. purple curve). The λ β K214A single-amino acid substitution also has a significantly impaired activity (green curve). In addition, we tested Q8KQW0 from Vibrio cholera, the most active recombinase from our initial library. Q8KQW0 showed moderate activity in the SSB-coated oligo annealing assay (blue curve).

FIG. 6A-6B depict that co-expression of species-matched SSAP-SSB pairs enable gain of recombinase function. FIG. 6A: To test the plausibility of bi-cistronic expression in an L-arabinose-based inducible pARC8 vector, a vector was synthesized that conferred both λ β and a spectinomycin resistance and the growth of that construct was tested under inducing conditions. Ecnr2 is the positive control in which the addition of arabinose produces no difference than with spectinomycin alone. The Beta.Spec bi-cistronic vector grows in the presence of arabinose (dark blue curve) shows increased expression than when it is simply induced with spec, alone. FIG. 6B: Given (A), bi-cistronic variants were constructed containing the indicated SSAP and the indicated SSB. These plasmid variants were transformed into the SEER chassis and performed-arabinose-based oligo recombinations to fix tolC, and quantified Allele Recombination (AR) Frequency as the number of colonies on selective media (SDS) divided by the number of colonies on non-selective media (carb). These data are presented as the mean AR Frequency and the error bars indicate the Std. Error of the Mean, and are the result of at least 4 biological replicates. Here, Lr=L. reuteri (light gray), Cg=C. glutamicum (dark gray), Ec=E. coli (white). The black column indicates the frequency of AR frequency of recombinase-deficient clones electroporated with the same oligos (background).

FIG. 7A-7D depict data regarding synthesis of metagenomic recombinase homologs.

FIG. 8A-7C depict data of results of recombinase discovery using the SEER method described herein in E. coli.

FIG. 9 depict data regarding synthesis of metagenomic recombinase homologs.

DETAILED DESCRIPTION

The present disclosure provides methods of in vivo or ex vivo recombination-mediated genetic engineering including providing a cell, such as a prokaryotic cell or eukaryotic cell, with a recombinase and a single strand binding protein (i.e., single strand nucleic acid binding protein or a single strand DNA binding protein) and a donor nucleic acid (i.e., a single stranded nucleic acid, a single stranded DNA, a double stranded nucleic acid or a double stranded DNA), wherein either one or both of the recombinase and a single strand binding protein are foreign to the cell in which they are present. The pair of the recombinase and the single strand binding protein, in combination with the host cell's translational machinery, is sufficient to insert an ssDNA sequence into a target nucleic acid sequence within the cell.

The present disclosure provides methods of in vitro recombination-mediated genetic engineering including providing in a suitable in vitro environment a target nucleic acid sequence, a target cell's translational machinery (i.e., those proteins and other components responsible for translation within the cell), a recombinase and a single strand binding protein (i.e., single strand nucleic acid binding protein or a single strand DNA binding protein) and a donor nucleic acid (i.e., a single stranded nucleic acid, a single stranded DNA, a double stranded nucleic acid or a double stranded DNA), wherein either one or both of the recombinase and a single strand binding protein are foreign to the cell. The pair of the recombinase and the single strand binding protein, in combination with the host cell's translational machinery, is sufficient to insert an ssDNA sequence into a target nucleic acid sequence.

Cells according to the present disclosure include any cell into which foreign nucleic acids can be introduced and expressed as described herein. It is to be understood that the basic concepts of the present disclosure described herein are not limited by cell type. Cells according to the present disclosure include eukaryotic cells, prokaryotic cells, animal cells, plant cells, fungal cells, bacteria cells, archaeal cells, eubacterial cells and the like. Cells include eukaryotic cells such as yeast cells, plant cells, and animal cells. Particular cells include mammalian cells and human cells. Particular cells include stem cells, such as pluripotent stem cells, such as human induced pluripotent stem cells.

Target nucleic acids include any nucleic acid sequence into which a donor nucleic acid can be inserted or introduced or otherwise included. Target nucleic acids include genes. For purposes of the present disclosure, DNA, such as double stranded DNA, can include the target nucleic acid. Such target nucleic acids can include endogenous (or naturally occurring) nucleic acids and exogenous (or foreign) nucleic acids. The target nucleic acid sequence may be replicating DNA such as genomic DNA, mitochondrial DNA, viral DNA, exogenous DNA, a plasmid, a bacteriophage genome and other replicating DNA known to those of skill in the art.

The donor nucleic acid includes any nucleic acid to be inserted into a nucleic acid sequence as described herein. Foreign or exogenous nucleic acids (i.e. those which are not part of a cell's natural nucleic acid composition) may be introduced into a cell using any method known to those skilled in the art for such introduction. Such methods include transfection, transduction, viral transduction, microinjection, lipofection, nucleofection, nanoparticle bombardment, transformation, conjugation and the like. One of skill in the art will readily understand and adapt such methods using readily identifiable literature sources.

Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described in Sambrook, J., Fritsch, E. F. and Maniatis, T., Molecular Cloning: A Laboratory Manual, 2^nded.; Cold Spring Harbor Laboratory: Cold Spring Harbor, N.Y., (1989) and by Silhavy, T. J., Bennan, M. L. and Enquist, L. W., Experiments with Gene Fusions; Cold Spring Harbor Laboratory: Cold Spring Harbor, N.Y., (1984); and by Ausubel, F. M. et. al., Current Protocols in Molecular Biology, Greene Publishing and Wiley-Interscience (1987) each of which are hereby incorporated by reference in its entirety.

Additional useful methods are described in manuals including Advanced Bacterial Genetics (Davis, Roth and Botstein, Cold Spring Harbor Laboratory, 1980), Experiments with Gene Fusions (Silhavy, Berman and Enquist, Cold Spring Harbor Laboratory, 1984), Experiments in Molecular Genetics (Miller, Cold Spring Harbor Laboratory, 1972) Experimental Techniques in Bacterial Genetics (Maloy, in Jones and Bartlett, 1990), and A Short Course in Bacterial Genetics (Miller, Cold Spring Harbor Laboratory 1992) each of which are hereby incorporated by reference in its entirety.

Microorganisms may be genetically modified to delete genes or incorporate genes by methods known to those of skill in the art. Vectors and plasmids useful for transformation of a variety of host cells are common and commercially available from companies such as Invitrogen Corp. (Carlsbad, Calif.), Stratagene (La Jolla, Calif.), New England Biolabs, Inc. (Beverly, Mass.) and Addgene (Cambridge, Mass.).

Typically, the vector or plasmid contains sequences directing transcription and translation of a relevant gene or genes, a selectable marker, and sequences allowing autonomous replication or chromosomal integration. Suitable vectors comprise a region 5′ of the gene which harbors transcriptional initiation controls and a region 3′ of the DNA fragment which controls transcription termination. Both control regions may be derived from genes homologous to the transformed host cell, although it is to be understood that such control regions may also be derived from genes that are not native to the species chosen as a production host.

Initiation control regions or promoters, which are useful to drive expression of the relevant pathway coding regions in the desired host cell are numerous and familiar to those skilled in the art. Virtually any promoter capable of driving these genetic elements is suitable for the present invention including, but not limited to, lac, ara, tet, trp, IP_L, IP_R, T7, tac, and trc (useful for expression in Escherichia coli and Pseudomonas); the amy, apr, npr promoters and various phage promoters useful for expression in Bacillus subtilis, and Bacillus licheniformis; nisA (useful for expression in Gram-positive bacteria, Eichenbaum et al. Appl. Environ. Microbiol. 64(8):2763-2769 (1998)); and the synthetic P11 promoter (useful for expression in Lactobacillus plantarum, Rud et al., Microbiology 152:1011-1019 (2006)). Termination control regions may also be derived from various genes native to the preferred hosts.

Certain vectors are capable of replicating in a broad range of host bacteria and can be transferred by conjugation. The complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H) are available. These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria (Scott et al., Plasmid 50(1):74-79 (2003)). Several plasmid derivatives of broad-host-range Inc P4 plasmid RSF1010 are also available with promoters that can function in a range of Gram-negative bacteria. Plasmid pAYC36 and pAYC37, have active promoters along with multiple cloning sites to allow for the heterologous gene expression in Gram-negative bacteria.

Chromosomal gene replacement tools are also widely available. For example, a thermosensitive variant of the broad-host-range replicon pWV101 has been modified to construct a plasmid pVE6002 which can be used to create gene replacement in a range of Gram-positive bacteria (Maguin et al., J. Bacteriol. 174(17):5633-5638 (1992)). Additionally, in vitro transposomes are available to create random mutations in a variety of genomes from commercial sources such as EPICENTRE® (Madison, Wis.).

Vectors useful for the transformation of E. coli are common and commercially available. For example, the desired genes may be isolated from various sources, cloned onto a modified pUC19 vector and transformed into E. coli host cells. Alternatively, the genes encoding a desired biosynthetic pathway may be divided into multiple operons, cloned onto expression vectors, and transformed into various E. coli strains.

The Lactobacillus genus belongs to the Lactobacillales family and many plasmids and vectors used in the transformation of Bacillus subtilis and Streptococcus may be used for Lactobacillus. Non-limiting examples of suitable vectors include pAM.beta.1 and derivatives thereof (Renault et al., Gene 183:175-182 (1996); and O'Sullivan et al., Gene 137:227-231 (1993)); pMBB1 and pHW800, a derivative of pMBB1 (Wyckoff et al. Appl. Environ. Microbiol. 62:1481-1486 (1996)); pMG1, a conjugative plasmid (Tanimoto et al., J. Bacteriol. 184:5800-5804 (2002)); pNZ9520 (Kleerebezem et al., Appl. Environ. Microbiol. 63:4581-4584 (1997)); pAM401 (Fujimoto et al., Appl. Environ. Microbiol. 67:1262-1267 (2001)); and pAT392 (Arthur et al., Antimicrob. Agents Chemother. 38:1899-1903 (1994)). Several plasmids from Lactobacillus plantarum have also been reported (van Kranenburg R, Golic N, Bongers R, Leer R J, de Vos W M, Siezen R J, Kleerebezem M. Appl. Environ. Microbiol. 2005 March; 71(3): 1223-1230), which may be used for transformation.

Initiation control regions or promoters, which are useful to drive expression of the relevant pathway coding regions in the desired Lactobacillus host cell, may be obtained from Lactobacillus or other lactic acid bacteria, or other Gram-positive organisms. A non-limiting example is the nisA promoter from Lactococcus. Termination control regions may also be derived from various genes native to the preferred hosts or related bacteria.

The various genes for a desired pathway may be assembled into any suitable vector or vectors, such as those described above. A single vector need not include all of the genetic material encoding a complete pathway. One or more or a plurality of vectors may be used in any aspect of genetically modifying a cell as described herein. The codons can be optimized for expression based on the codon index deduced from the genome sequences of the host strain, such as for Lactobacillus plantarum or Lactobacillus arizonensis. The plasmids may be introduced into the host cell using methods known in the art, such as electroporation, as described in any one of the following references: Cruz-Rodz et al. (Molecular Genetics and Genomics 224:1252-154 (1990)), Bringel and Hubert (Appl. Microbiol. Biotechnol. 33: 664-670 (1990)), and Teresa Alegre, Rodriguez and Mesas (FEMS Microbiology Letters 241:73-77 (2004)). Plasmids can also be introduced to Lactobacillus plantatrum by conjugation (Shrago, Chassy and Dobrogosz Appl. Environ. Micro. 52: 574-576 (1986)). The desired pathway genes can also be integrated into the chromosome of Lactobacillus using integration vectors (Hols et al. Appl. Environ. Micro. 60:1401-1403 (1990); Jang et al. Micro. Lett. 24:191-195 (2003)).

Microorganisms which may serve as host cells and which may be genetically modified to produce recombinant microorganisms as described herein may include one or members of the genera Clostridium, Escherichia, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus Saccharomyces, and Enterococcus. Particularly suitable microorganisms include Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae.

Exemplary genus and species of bacteria cells for use in the methods described herein, for use in identifying corresponding phage, or for otherwise carrying out recombination-mediated genetic engineering include Acetobacter aurantius, Acinetobacter bitumen, Actinomyces israelii, Agrobacterium radiobacter, Agrobacterium tumefaciens, Anaplasma Anaplasma phagocytophilum, Azorhizobium caulinodans, Azotobacter vinelandii, viridans streptococci, Bacillus anthracis, Bacillus brevis, Bacillus cereus, Bacillus fusiformis, Bacillus licheniformis, Bacillus megaterium, Bacillus mycoides, Bacillus stearothermophilus, Bacillus subtilis, Bacteroides, Bacteroides fragilis, Bacteroides gingivalis, Bacteroides melaninogenicus (also referred to as Prevotella melaninogenica), Bartonella, Bartonella henselae, Bartonella quintana, Bordetella, Bordetella bronchiseptica, Bordetella pertussis, Borrelia burgdorferi, Brucella abortus, Brucella melitensis, Brucella suis, Burkholderia, Burkholderia mallei, Burkholderia pseudomallei, Burkholderia cepacia, Calymmatobacterium granulomatis, Campylobacter, Campylobacter coli, Campylobacter fetus, Campylobacter jejuni, Campylobacter pylori, Chlamydia, Chlamydia trachomatis, Chlamydophila Chlamydophila pneumoniae (also known as Chlamydia pneumoniae) Chlamydophila psittaci (also known as Chlamydia psittaci), Clostridium, Clostridium botulinum, Clostridium difficile, Clostridium perfringens (also known as Clostridium welchii), Clostridium tetani, Corynebacterium, Corynebacterium diphtheriae, Corynebacterium fusiforme, Coxiella burnetii, Ehrlichia chaffeensis, Enterobacter cloacae, Enterococcus, Enterococcus avium, Enterococcus durans, Enterococcus faecalis, Enterococcus faecium, Enterococcus galllinarum, Enterococcus maloratus, Escherichia coli, Francisella tularensis, Fusobacterium nucleatum, Gardnerella vaginalis, Haemophilus, Haemophilus ducreyi, Haemophilus influenzae, Haemophilus parainfluenzae, Haemophilus pertussis, Haemophilus vaginalis, Helicobacter pylori, Klebsiella pneumoniae, Lactobacillus, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Lactococcus lactis, Legionella pneumophila, Listeria monocytogenes, Methanobacterium extroquens, Microbacterium multiforme, Micrococcus luteus, Moraxella catarrhalis, Mycobacterium, Mycobacterium avium, Mycobacterium bovis, Mycobacterium diphtheriae, Mycobacterium intracellulare, Mycobacterium leprae, Mycobacterium lepraemurium, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, Mycoplasma, Mycoplasma fermentans, Mycoplasma genitalium, Mycoplasma hominis, Mycoplasma penetrans, Mycoplasma pneumoniae, Neisseria, Neisseria gonorrhoeae, Neisseria meningitidis, Pasteurella, Pasteurella multocida, Pasteurella tularensis, Peptostreptococcus, Porphyromonas gingivalis, Prevotella melaninogenica (also known as Bacteroides melaninogenicus), Pseudomonas aeruginosa, Rhizobium radiobacter, Rickettsia, Rickettsia prowazekii, Rickettsia psittaci, Rickettsia quintana, Rickettsia rickettsii, Rickettsia trachomae, Rochalimaea, Rochalimaea henselae, Rochalimaea quintana, Rothia dentocariosa, Salmonella, Salmonella enteritidis, Salmonella typhi, Salmonella typhimurium, Serratia marcescens, Shigella dysenteriae, Staphylococcus, Staphylococcus aureus, Staphylococcus epidermidis, Stenotrophomonas maltophilia, Streptococcus Streptococcus agalactiae, Streptococcus avium, Streptococcus bovis, Streptococcus cricetus, Streptococcus faceium, Streptococcus faecalis, Streptococcus ferus, Streptococcus gallinarum, Streptococcus lactis, Streptococcus mitior, Streptococcus mitis, Streptococcus mutans, Streptococcus oralis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus rattus, Streptococcus salivarius, Streptococcus sanguis, Streptococcus sobrinus, Treponema, Treponema pallidum, Treponema denticola, Vibrio, Vibrio cholerae, Vibrio comma, Vibrio parahaemolyticus, Vibrio vulnificus, Wolbachia, Yersinia, Yersinia enterocolitica, Yersinia pestis, and Yersinia pseudotuberculosis, and other genus and species known to those of skill in the art.

Exemplary genus and species of yeast cells for use in the methods described herein, or for otherwise carrying out recombination-mediated genetic engineering include Saccharomyces, Saccharomyces cerevisiae, Torula, Saccharomyces boulardii, Schizosaccharomyces, Schizosaccharomyces pombe, Candida, Candida glabrata, Candida tropicalis, Yarrowia, Candida parapsilosis, Candida krusei, Saccharomyces pastorianus, Brettanomyces, Brettanomyces bruxellensis, Pichia, Pichia guilliermondii, Cryptococcus, Cryptococcus gattii, Torulaspora, Torulaspora delbrueckii, Zygosaccharomyces, Zygosaccharomyces bailii, Candida lusitaniae, Candida stellata, Geotrichum, Geotrichum candidum, Pichia pastoris, Kluyveromyces, Kluyveromyces marxianus, Candida dubliniensis, Kluyveromyces, Kluyveromyces lactis, Trichosporon, Trichosporon uvarum, Eremothecium, Eremothecium gossypii, Pichia stipitis, Candida milleri, Ogataea, Ogataea polymorpha, Candida oleophilia, Zygosaccharomyces rouxii, Candida albicans, Leucosporidium, Leucosporidium frigidum, Candida viswanathii, Candida blankii, Saccharaomyces telluris, Saccharomyces florentinus, Sporidiobolus, Sporidiobolus salmonicolor, Dekkera, Dekkera anomala, Lachancea, Lachancea kluyveri, Trichosporon, Trichosporon mycotoxinivorans, Rhodotorula, Rhodotorula rubra, Saccharomyces exiguus, Sporobolomyces koalae, and Trichosporon cutaneum, and other genus and species known to those of skill in the art.

Exemplary genus and species of fungal cells for use in the methods described herein, or for otherwise carrying out recombination-mediated genetic engineering include Sac fungi, Basidiomycota, Zygomycota, Chtridiomycota, Basidiomycetes, Hyphomycetes, Glomeromycota, Microsporidia, Blastocladiomycota, and Neocallimastigomycota, and other genus and species known to those of skill in the art.

Exemplary recombinases for use in the recombineering methods described herein are listed in Tables 1-6. Exemplary single strand binding proteins for use in the recombineering methods described herein are listed in Table 7. Table 8 is an exemplary list of single stranded binding homologs corresponding to the protein sequences referenced by Uniprot IDs. Exemplary pairs of single strand binding proteins and recombinases include SSB (WP_003669492.1) and DNA recombination protein 1 from Lactobacillus reuteri (WP_003668036.1); SSB (WP_011835834.1) from Lactococcus lactis and phage recombination protein bet from lactococcus phage phi31.1; SSB (WP_011015545.1) from Corynebacterium glutamicum and gp61 (NP_817738.1) from Mycobacteriophage Che9c; SSB (WP_003400534.1) from Mycobacterium tuberculosis and gp61 (NP_817738.1) from Mycobacteriophage Che9c; SSB (WP_011269089.1) and recT (YP_235897.1) from Pseudomonas syringae; and SSB (CQR83440.1) and λ β itself from Escherichia coli K-12 sp MG1655.

The disclosure provides the use of Multiplex Automated Genome Engineering (MAGE) to enable multiplexed genomic mutations in Escherichia coli. See Wang, H. H. et al. 2009. Programming cells by multiplex genome engineering and accelerated evolution. Nature. 460, 7257 (August 2009), 894-8 hereby incorporated by reference in its entirety; Wang, H. H. et al. 2012. Genome-scale promoter engineering by co-selection MAGE. Nature methods. 9, 6 (June 2012), 591-3; Lajoie, M. J. et al. 2013. Probing the limits of genetic recoding in essential genes. Science (New York, N.Y.). 342, 6156 (October 2013), 361-3; Lajoie, M. J. et al. 2013. Genomically recoded organisms expand biological functions. Science (New York, N. Y). 342, 6156 (October 2013), 357-60 each of which is hereby incorporated by reference in its entirety.

The disclosure provides the use of MAGE with the λ Red recombinase, λ β (Bet), a viral recombinase or homologs thereof or proteins having similar function to λ β (Bet), that when ectopically expressed improves the efficiency of recombination of single-stranded DNA oligonucleotides into the bacterial genome. See Lajoie, M. J. et al. 2012. Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering. Nucleic acids research. 40, 22 (December 2012), e170 hereby incorporated by reference in its entirety. The disclosure provides a method referred to herein as Serial Evolutionary Enrichment for Recombinases (SEER) that enables the rapid discovery of Bet variants for use with MAGE in certain prokaryotic strains. A library of Bet homologs was built with homology searches across all known prokaryotic proteins, and curated to ensure large diversity (200 homologs). This library was then subjected to six successive rounds of selection in E. coli for improved recombineering activity, and characterized. Improved Bet homologs may be used for recomineering in Escherichia coli, Lactobacillus reuteri and Corynebacterium glutamicum.

The present disclosure provides that the molecular basis of Bet's recombinase function includes interaction with E. coli's single-stranded binding protein (SSB). Bet acts to specifically unload SSB from SSB-coated single-stranded DNA (ssDNA). This then enables strand-strand annealing, which is the mechanism by which ssDNA is incorporated into the replication fork in Bet-mediated recombineering. The present disclosure provides that an SSB taken from the same host organism as the Bet recombinase homolog improves the functioning of the Bet homolog in E. coli. Accordingly, the recombinase and/or the single strand binding protein are foreign to the cell in which they are present while facilitating incorporation of a donor nucleic acid into a target nucleic acid.

The following examples are set forth as being representative of the present disclosure. These examples are not to be construed as limiting the scope of the present disclosure as these and other equivalent embodiments will be apparent in view of the present disclosure, figures and accompanying claims.

Example I
Materials and Methods
Strains & Culture Methods

The strains used in this work were derived from EcNR2 (EcNR2.dnaG_Q576A.tolC_mut.mutS::cat_mut.dlambda::zeoR) (Wang et al., 2009). Strains were grown in liquid culture using the Lennox formulation of lysogeny broth (LB^L) (Lennox, 1955) with the appropriate selective agents: carbenicillin (50 μg/mL), chloramphenicol (20 μg/mL), SDS (0.005% w/v), zeocin (100 μg/mL).

Oligonucleotides, Polymerase Chain Reaction, Quikchanges, and Isothermal Assembly

Oligonucleotides were identified. PCR products used in transformations and recombinations were amplified using Kapa Biosystems, High-Fidelity polymerase, according to the manufacturer's instructions. Kapa 2G Fast ready mix was used to PCR screen the correct insertion in strains. Sanger sequencing of PCR products was carried out through a 3^rdparty service (Genewiz, Inc.). To assemble multiple DNA sequences into a single contiguous sequence, or to assemble a circularized vector from linear vector backbone and insertion variants, isothermal assembly at 50° C. for 60 minutes was used based on published protocols (Gibson et al., 2009). When SNV variants of a given plasmid system (e.g., to introduce point mutations) were desired, the QuikChange II Lightning Kit (Agilent Technologies) was used with primers encoding the mutations of interest to generate the mutant strand, followed by dpnI-digest of the parental plasmid, according to the manufacturer's instructions.

Transformations, A Red Recombinations, & MAGE

Transformations were conducted with Zymo Research's Mix & Go DH5a Z-competent E. coli, according to the manufacturer's protocol, except for the recovery step where the culture was recovered in 1 mL of LB^Lfor 1 hour before plating onto appropriate medium.

For recombinase discovery using the SEER method described herein and characterization, λ Red recombineering was implemented on episomal expression vector using 0.2% D-glucose to repress and 0.2% L-arabinose to induce expression (Datsenko and Wanner, 2000). An overnight growth culture was passaged 1:100 into 3 mL LB^Lwith 0.2% D-glucose. The cultures were then incubated at 34 C with rotation until the OD₆₀₀˜0.1 (˜1 hour). 2 mL culture was then washed twice with 2 mL water and resuspended in 2.5 mL LB^Lwith 0.2% L-arabinose. The cultures were then induced for 45 minutes while rotating at 34° C. followed by icing the culture and washing 0.980 mL of culture twice in ice-cold sterile water. Thereafter, pellets were resuspended with 50 μl of 1 μM ssDNA oligo, or 100 ng dsDNA PCR product, or both depending on the goal of the recombination. Cultures were electroporated at 1.8 kV, 200 ohms, 25 uF and recovered in 2 mL of LB^Lfor 2 hours.

Synthesis of Metagenomic Recombinase Libraries

To generate a library of candidate SSAPs, two approaches to cataloging metagenomic homologs of λ β were pursued. For the first, an iterative PSI-blast was performed using λ (NP_040617.1) as the query. Candidates exhibited a bi-modal distribution where the first was SSAP-like, with sequence lengths from 500-1,050 bp (except for 4 candidates >1,050 bp.), and were annotated as recombination protein or unknown. The second were larger genes (1,200-1,500+bp), and largely annotated as ABC-related ATP binding cassettes. The latter were removed. Any SSAP-like candidates from E. coli were removed to minimize redundancy with λ β. Identical entries were removed.

In the second approach, multiple sequence alignment of NP_040617.1 (λ β), NP_930169.1 from Photorhabdus luminescens, Q9AKZ0 from Legionella pneumophila, Q8KQW0 from Virbio cholerae, Q9MBV8 from Lactococcus phage u136.2, YP_003084246.1 from Prochlorococcus siphovirus P-SS2, NP_815795.1 (EF2132) from Enterococcus faecalis, and NP_463513.1 from Listeria phage A118 were used to generate a Hidden Markov Model that described the weighted positional variance of these proteins. Non-redundant nucleotide and environmental metagenomic databases were queried using web-based search interface (Finn et al., 2011). Candidates were filtered based on gene size and ABC ATP-binding cassette annotation. Candidates that exhibited intra-sequence similarity of greater than 98% were removed from the group.

A number of other recombinases were added for synthesis, including candidates from other model organisms that have been previously shown to function in E. coli (NP_930169.1 from Photorhabdus luminescens, Q9AKZ0 from Legionella pneumophila, Q8KQW0 from Virbio cholerae, Q9MBV8 from Lactococcus phage u136.2, YP_003084246.1 from Cyanophage P-SS2, NP_815795.1 (EF2132) from Enterococcus faecalis (Datta et al., 2008), recT from E. coli K12 (B1XAU6), CG19468 from Drosophila Melanogaster (Eisen and Camerini-Otero, 1988), C7F4E8 from Prochlorococcus siphovirus P-SS2 (Sullivan et al., 2009), and NP_040617.1 (λ β itself) (P03698). In total, a library was created that contained 72 members from the first approach, 113 members from the second, and 10 members that were rationally added for a total of 195 recombinase homologs.

To prepare the library for synthesis (Gen9, Inc.), the protein coding sequence was reverse translated using optimized codon usage tables for E. coli. Upper bounds (>70%) and lower bounds (<30%) for GC-content of 100-mer windows were set and codon usage was manually messaged to meet these requirements. ATG was used for all starts codons. TAA was used for all stop codons. Upstream of the coding sequence, 35 bp of homology was added to support assembly with the pARC8 (Choe et al., 2005) vector (5′-TTCTCCATACCTGTTTTTCTGGATGGAGTAAGACC-3′)(SEQ ID NO:1). After the stop codon, the primer sequence of a Illumina-like primer, barcode region of interest that was unique to each library member, and the hybridization site for a reverse Illumina primer to support a PCR-based library preparation for high-throughput, Illumina sequencing was added (see below). Downstream of the barcode region, 35 bp of homology was added to support assembly with the pARC8 vector (5′-ACTAGTGGGGAAGCTTATCGATGATAAGCTGTCAA-3′)(SEQ ID NO:2). As a final synthesis requirement, synthons were manually redesigned, as needed, to avoid the following sequences: GGGGG, AAAAAAAA, CCCCCCCC, TTTTTTTT, GGTCTC, GAGACC.

Sythons were pooled at equimolar ratios and assembled in a complex isothermal assembly (Gibson et al., 2009) using a linear pARC8 vector backbone, which enabled episomal expression of the recombinase candidates under 0.2% L-arabinose at a copy number ˜10. Crude assemblies were transformed into Z-competent DH5a (Zymo Research) and plated onto LB^Lagar containing carbenicillin to generate sufficient colonies for at least 10× coverage. The colonies were counted and scraped into LB^Lplus carbenicillin for plasmid preparation. To verify the diversity of any library preparation, a plasmid-limiting transformation of the complex plasmid preparation into naïve DH5a using 1 ng, 100 pg, 10 pg, and 1 pg of plasmid was performed, followed by plating onto selective agar. 96 clones were picked from the most plasmid-limited conditions and inserts were identified using the barcode region of interest.

Serial Evolutionary Enrichment for Recombinases (SEER)

To perform SEER (e.g., enrich for functional recombinases), oligo recombinations were leveraged to restore the coding region of a broken selectable marker followed by the respective selection as the mechanistic foundation for enrichment. In E. coli, multiple markers were used and inactivated, as such used MAGE to inactivate tolC^WT, mutS::cat, and 1984000::gfp_mut3b using oligo recombinations, followed by asPCR screening or replica plating to isolate the inactivated clones. These three inactivations generated EcNR2.mutS::cat_mut.tolC^WT_mut.1984000::gfp_mut3b_mut, which still contained the λ prophage and is competent for recombination. The entire prophage was then deleted in a dead-end recombination using a Δλ::zeoR PCR cassette, followed by selection on LB^Lagar plus zeocin to create a recombinase-deficient chassis for SEER in E. coli.

After transforming the pARC8-based libraries into the naive SEER chassis, the libraries were induced using 0.2% L-arabinose and oligo recombinations were performed to fix a broken selectable marker or a mock, water-only recombination was performed. The tolC^WT_mut was fixed using tolC-Lnull_revert, followed by selection in LB^L+SDS. The mutS::cat_mut was fixed using cat_CS_restore followed by selection in LB^L+Cm. These two oligo recombination/selection steps constitute 2 Rounds of Enrichment (RoE), but exhausted the opportunities for selectable recombinations in the chassis. To continue, total plasmid preps were prepared from post-selection cultures after 2 RoE, and the preparation was transformed into a naïve chassis to conduct additional RoE's and facilitate convergence onto a solution.

High-Throughput Sequencing

To support rapid and deep identification of recombinases, a custom Illumina sequencing platform was designed to leverage high-fidelity PCR to amplify the barcode region directly using large library size. After the stop (TAA), the seed sequence for barcoded Illumina p7 forward adapter (GACGTGTGCTCTTCCGATCT)(SEQ ID NO:3) was added, followed by two tandem 6-mer library IDs (cNNNNNNgNNNNNN)(SEQ ID NO:4), followed by the hybridization site for p5_alt (GATCGCCTAGACAACTCCTGA)(SEQ ID NO:5), a custom sequence chosen for minimal secondary structure (Kosuri et al., 2010; Xu et al., 2009). The p5_alt hyb site binds the barcoded, Illumina-compatible p5_alt reverse adapter, supporting robust amplification with few cycles. Libraries were amplified with Phusion (New England Biolabs) at 100 μL scale containing genomes from 10 μL of post-selection culture (10⁷-10⁸unique clones) for 10-16 cycles. The expected amplicon size is 146 bp and follows the format 5′-AATGATACGGCGACCACCGAGATCTACACnnnnnnACACTCTTTCCCTCAGGAGTT GTCTAGGCGATCcNNNNNNgNNNNNNAGATCGGAAGAGCACACGTCTGAACTCC AGTCACnnnnnnATCTCGTATGCCGTCTTCTGCTTG-3′ (SEQ ID NO:6), where nnnnnn are 6-mer indices added in the PCR reaction (see Table S4) (Gregg et al., 2014). Magnetic bead-associated PEG was used to cleanup reactions (Rohland and Reich, 2012). The libraries were visualized for specificity and pooled to equimolar amounts depending on the number of indices (unique experimental conditions) being sequenced. MiSeq SE50 runs were carried out using the custom read primer (ACACTCTTTCCCTCAGGAGTTGTCTAGGCGATC)(SEQ ID NO:7) and standard indexing primer, and included a 30% PhiX spike-in to mitigate sequencing of largely constant regions. A diagram depicting the entire SEER workflow is shown at FIG. 1.

Protein Purification

pARC8 was also leveraged for in vitro characterization and recombinant recombinase production. After cloning via isothermal assembly (Gibson et al., 2009) and adding an N-terminal 6×His tag (SEQ ID NO: 108) onto candidate proteins, the vector was transformed into NiCo21(DE3) competent E. coli (New England Biolabs). For protein production, 50 mL LB^Lplus 25 μg/mL chloramphenicol was inoculated with 1:100 from overnight confluent cultures, themselves grown LB^Lplus chloramphenicol plus 0.2% D-glucose. The 50 mL cultures were grown for 6 hours at 37° C. in LB^L+chloramphenicol, then induced using 0.1% L-arabinse. Cultures were spun down at 5,000 g for 10 minutes at 4° C. and the pellets were snap frozen in a dry ice ethanol bath. The pellets were thawed, then lysed using P-BER with Enzymes (Thermo Scientific) for 10 minutes at room temperature, according to the manufacturer's instructions. Lysates were mixed 1:1 with binding buffer (40 mM Imidazole, 500 nM NaCl, 50 mM Tris pH 7.4), spun down 10 mins 5,000 g 4° C. and the soluble fraction was added to a 20 mL column pre-loaded with 2 mL His GraviTrap Ni-NTA resin (GE Healthcare) that was pre-equilibrated with binding buffer. After binding, the columns were washed twice with 20 mL of wash buffer (100 mM Imidazole, 500 nM NaCl, 50 mM Tris pH 7.4), then eluted with 4 mL of elution buffer (500 mM Imidazole, 500 nM NaCl, 50 mM Tris pH 7.4) in 1 mL fractions. Protein concentration was quantified using the Qubit system (Life Technologies), and stability and purity was checked by SDS-PAGE (Bio-rad). The purest, most concentrated fractions were pooled and buffer exchanged with Zeba desalting columns 7K MWCO (ThermoFisher Scientific) into storage buffer (200 nM NaCl, 50 mM Tris pH 7.4, 1 mM DTT). Protein preps were concentrated using Amicon Ultra-4 10K centrifugal filters (Millipore), as needed.

Oligonucleotide Quenching Assay

Fluorophore/quencher complementary oligos were ordered from IDT (5′-AGCAAGCACGCCTTAGTAACCCGGAATTGCGTAAGTCTGCCGCCGATCGTGATG CTGCCTTTGAAAAAATTAATGAAGCGCGCAGTCCA/6-FAM/-3′ (SEQ ID NO:8) and 5′-/IABkFQ/TGGACTGCGCGCTTCATTAATTTTTTCAAAGGCAGCATCACGATCGGCG GCAGACTTACGCAATTCCGGGTTACTAAGGCGTGCTTGCT-3′ (SEQ ID NO:9). For the SSB annealing assays, the 10 nM oligo solutions with 20 nM NaCl, 1 mM DTT, 50 mM Tris pH 7.4 were separately incubated with 500 nM SSB or SSBAC8 for 20 minutes at 37 C. The reactions were tracked in a Synergy H4 Hybrid Microplate Reader (Biotek) in half-area, low-bind black 96 well plates. The oligos were serially added to the plate, then the SSAP in the same buffer.

Fluorescence Quenching-Based Annealed Fraction Estimate:

I=Fluorescence intensity at a given time is

I=F
_f
I
_f
+F
_b
I
_b

Where F_f=Free fraction, I_f=Free intensity, F_b=Bound fraction, I_b=Bound fraction. For a DNA annealing assay, F_fis the substrate, and F_bis the product

$\begin{matrix} At t_{o} \to F_{f} = 1, F_{b} = 0 \\ F_{f} + F_{b} = 1; F_{f} = 1 - F_{b} so \\ I = I_{f} (1 - F_{b}) + F_{b} I_{b} \\ F_{b} = \frac{I - I_{f}}{I_{b} - I_{f}} = 1 - \frac{I - I_{b}}{I_{f} - I_{b}} . \end{matrix}$

This calculation is independent of experimental background (B) from the reader:

$F_{b} = \frac{(I - B) - (I_{f} - B)}{(I_{b} - B) - (I_{f} - B)} = \frac{I - I_{f}}{I_{b} - I_{f}}$

I_bwas estimated from the minimal steady-state fluorescence of annealed oligos in the presence of protein, while I_fwas measured in parallel for each reaction using an unlabeled oligo instead for the quencher. This helped control for the variable background fluorescence of different protein solutions and the fluorescence decay of the FITC fluorophore over the time course measured. The reactions were tracked for an hour, measuring every 7 seconds. The naked-oligo experiments were done in a similar way, except no SSB was added during the pre-incubation step. Annealing and steady-state graphs were generated using GraphPad Prism 5.

Example II
Analysis of SEER Library 1 in E. coli

Phylogenetic analyses suggest that phage-derived SSAPs belong to six distinct families: redβ, erf, sak, sak4, uvsX, & gp2.5 (Iyer et al., 2002; Lopes et al., 2010). These recombinases are present in a variety of phages that exhibit both temperate and lytic lifestyles. The disclosure generates SSAP libraries that widely sample potential sequence space. An Iterated PSI-BLAST was used with the λ β amino acid sequence as the query, which produced a list of 500 candidates. From the initial hits, β homologs were removed from E. coli-derived phages, which were often identical to the query (e.g., E-value˜1⁻¹⁰⁴). By plotting the logarithm of the inverse of the E-value fit score for each candidate versus the rank score (FIG. 7A), it was visualize how the Goodness of Fit varied throughout the total list. In order to maximize the versatility of the library, both the highest ranked candidates and the tail-end of the distribution were sampled. A clear bi-modal distribution of predicted Polypeptide Length versus Rank Score (FIG. 7B) was identified, with the best hits (1st and 2nd deciles) exhibiting amino acid lengths around that of λ β (261 AA), whereas poorer hits (beyond the 2nd decile) were dominated by proteins of predicted length near 500 AA, which were largely annotated as ABC-related ATP binding cassettes. Sample hits <400 AA were then analyzed, regardless of Goodness of Fit, which are marked in light blue over the total candidate pool, marked in red (see FIG. 7A, 7B). This produced a pool of 77 candidates, of which 72 were successfully synthesized. Phylogenetic analysis suggested that the vast majority of these candidates fell into the redβ clade (0.943), except two homologs from the sak clade (P06778, NP005253777.1), two homologs from sak4 (YP_005321079.1, YP_002417106.1), and one from erf (YP_003467094.1). 10 homologs that have been previously shown to function in E. coli or other organisms (Datta et al., 2008; Eisen and Camerini-Otero, 1988; Sullivan et al., 2009) were synthesized. To understand the bias that known recombinases might impart on recombinase discovery, multiple library configurations which did not contain any known recombinases (Library 1.1), which contained the whole library minus λ β (Library 1.2), and which contained all 82 (including λ β, Library 1.3) were created. To perform SEER (see FIG. 1), 6 Rounds of Enrichment (RoE) were carried out with all three libraries and homologs were identified by Sanger sequencing.

Sequencing showed that inclusion of known recombinases dramatically altered the solution at the 6th RoE (FIG. 2A), though the coarse distribution of recombinases was unchanged with or without λ β (FIG. 2A, compare 1.2 versus 1.3). The candidate top hits from Library 1.2/1.3 (NP_930169.1 from Photorhabdus luminescens, Q9AKZ0 from Legionella pneumophila, Q8KQW0 from Vibrio cholerae), were assembled along with NP_040617.1 (λ β), and two poorly-functional control recombinases from Library 1.1 (YP_003993926.1 from Halanaerobium hydrogeniformans, NP_815795.1 from Enterococcus faecalis) for direct quantification of allele recombination frequency (FIG. 2B). These experiments showed that Q8KQW0 from Vibrio cholerae performed significantly better at oligo recombination in E. coli (0.20±0.04, mean±SEM, **p=0.008 vs. NP_040617.1 [λ β]) than λ β (0.06±0.01). The other two candidates, NP_930169.1 (0.11±0.04, p=0.79) & Q9AKZ0 (0.13±0.03, p=0.116) were equivalent to λ β, suggesting multiple potential alternatives to λ β in E. coli. These findings quantitatively contrast previous work (Datta et al., 2008) where these particular recombinases performed significantly worse than λ β at oligo recombination in E. coli. Moreover, the same study showed that NP_815795.1 from Enterococcus faecalis performed slightly better than λ β in single test oligo recombinations at the galK locus, whereas in the present experiments, this recombinase is around 1 log worse (FIG. 2B, dark gray squares). Locus-to-locus variability (tolC on+strand at Chr. 3,176,137 here, versus galK on—strand at Chr. 789,202) and oligo length (90-mer's here, versus 70-mer's) may contribute to some of these differences.

The candidates were tested for toxicity using a kinetic growth assay of the candidates with and without L-arabinose induction. Doubling time was calculated and presented as the change without (‘−L-ara’) and with (‘+L-ara’) arabinose (FIG. 2C). Two negative controls were included that would not be expected to increase doubling time upon induction, pARC8.GFP (empty black circles) and an empty pARC8 vector (filled black circles). These pARC8 variants did not exhibit wildly different doubling times without arabinose (51.8±5.7 minutes, min: 40.5±0.5 minutes for GFP; max: 60.8±2.3 minutes for NP_040617.1 [λ β]), but do exhibit slower growth with arabinose (FIG. 2C). Candidates that support the highest recombination rate (λ β included, FIG. 2B) also exhibit the slowest doubling times upon induction (see squares), whereas less functional recombinases led to more modest increases in doubling time, and negative controls did not increase doubling time. These results demonstrate that some level of toxicity is accepted for robust recombination rates, and that the candidates identified offer desirable performance (FIG. 2B) with acceptable toxicity (FIG. 2C).

Example III
Deep Sequencing Analysis of SEER Library 2 in E. coli

To develop a broader resource for recombinase discovery in non-standard model organisms and to sample the entirety of sequence space, a Hidden Markov Model-based search strategy was developed using multiple known recombinases to generate the position matrix with which to search nucleotide databases. This search returned ˜2,500 candidates that exhibited similar Goodness of Fit (FIG. 7C) and Polypeptide Length relationships (FIG. 7D) as the first library. Again, E. coli homologs were removed. Genes encoding less than 400 amino acids were the focus. The remaining distribution from the top 4 quintiles was sampled. Before synthesis, redundancy (defined as >98% amino acid identity) was removed which created a second library of 120 unique members, of which 113 were successfully synthesized using the same synthon design as Library 1. This new library (n=113) was combined with Library 1.3 (n=82) to generate Library 2 (n=195). A phylogenetic analysis is presented to show the diversity of SSAP clades covered by this new expansive library (FIG. 3A). Although Library 1.3 was heavily populated by members of redβ (0.943), the new library (n=113) has more balanced representation of all clades: redβ (0.400); erf (0.191); gp2.5 (0.127); sak (0.109); sak4 (0.146); and uvsX (0.027).

After complex assembly and verification of diversity, Library 2 was transformed and SEER in E. coli was performed. After the 6th RoE, the recombinase barcodes from 1E7 cells were PCR amplified before the first and after each RoE using indexed primers and sequenced using an Illumina MiSeq. After de-multiplexing and mapping, between 90.7 and 94.8% of raw reads corresponding to each RoE identically mapped to a recombinase barcode, resulting in a minimum read depth of 3.4E5 reads (see the 2nd RoE in FIG. 8A). Across all RoE, every recombinase was identified at least once throughout the experimental course.

193 of 195 total recombinases in the pre-SEER (0th RoE) population were identified, accurately reflecting the expected distribution of the clades in this library (FIG. 8B, 8C): redβ (0.63); erf (0.12); gp2.5 (0.07); sak (0.07); sak4 (0.09); and uvsX (0.2). There is notable skew in the population of library members, including the most abundant 31 recombinases (top 16%) accounting for 69% of the total reads, indicating significant assembly bias of some members over others. Over-abundant members included ZP_03935819.1_12 (0.100 of total reads), YP_950640_20 (0.062), EHN141107.1 (0.044), NP_040617.1 (λ β, 0.033), and two others greater than 0.03 frequency of total. Despite this skew, only 3/31 over-abundant recombinases emerged from the 6th RoE with a frequency of greater than 0.001: ZP_03935819.1_12 (redβ clade), 0.112; YP_001552302 (erf clade), 0.009; and NP_040617.1 (λ β), 0.005. These results suggest that some assembly bias can be tolerated by the power of serial enrichment.

Throughout the SEER workflow, the population diversity dropped from 193 at the 0th RoE to 55 unique members after the 6th RoE (FIG. 8C), though not strictly monotonically. Sampling bias in the 4th RoE (1.82E6 total reads) provided extra depth to identify 12 additional recombinases over those identified in reads from the 3rd RoE (0.52E6 total reads). Notably, 32 of the recombinases identified in the 4th RoE occurred below the frequency of detection of the 3rd RoE (1.91E-6), thus it is feasible to conclude that population diversity monotonically decreases with more RoE. Finally, relative clade abundance shifts in favor of redβ (FIG. 8B), whose members account for 0.639 of all reads at the 0th RoE and 0.840 of total at both the 5th and 6th RoE. At the 6th RoE, the erf clade accounts for 0.109 of total diversity, while sak4 (0.036) and gp2.5 (0.018) are also detected. Members of the uvsX clade are not detected after the 4th RoE, though at least 2 members were detected before that point, and were likely removed from the population by a sporadic spike in NP_040617.1 (λ β) abundance (0.645) at the 4th RoE. The abundance of NP_040617.1 (λ β) throughout the workflow (0.033, 0.017, 0.074, 0.010, 0.645, 0.033, 0.005, and Table 1) reflects this stark spike, however the population diversity doesn't reflect a bottleneck as diversity doesn't drop dramatically at the 4th RoE (FIG. 8C).

In terms of sheer abundance after the 6th RoE, desirable homologs included three redβ homologs, themselves accounting for 0.960 of all mapped reads: ZP_07797103.1 from Pseudomonas aeruginosa 39016, 0.556; ZP_09377516.1 from Hafnia alvei ATCC 51873, 0.292; and ZP_03935819.1 from Corynebacterium striatum ATCC 6940, 0.112 (FIG. 3A, Table 1). Using a phylogenetic analysis of the entire library (FIG. 3A), pair-wise comparisons of all members based on the branch length of the Newick string were computed. This showed that the 3 desirable homologs were in the 1st, 6th, and 6th closest deciles to NP_0406171.1 (λ β), highlighting the unpredictable nature of choosing candidate recombinases based on identity. The 6th, 7th, and 9th-most abundant recombinases at the 6th RoE (Table 1) were Q8KQW0 from Vibrio cholerae (0.009), NP_040617.1 (λ (3, 0.009), and Q9AKZ0 from Legionella pneumophila (0.005), corroborating results of SEER using Library 1.3 (FIG. 2A), and suggesting that desirable homologs from Library 2 may be equivalent or even better than λ β and/or Q8KQW0.

Enrichment factor (defined as freq_n/freq₀at nth RoE) is another way to consider relative performance that is less subject to skew at the 0th RoE (Table 2). At the 6th RoE, only 6 recombinases exhibited enrichment factors greater than 1.0 (Table 2), led by ZP_09377516.1 (170.1-fold) and ZP_07797103.1 (91.6-fold) that were the #2 and #1 most abundant recombinases at the 6th RoE. Also exhibiting enrichment factors >1 at the 6th RoE were Q8KQW0 (34.6-fold), Q9AKZ0 (2.80-fold), and ZP_08900554.1 from Gluconacetobacter oboediens 174Bp2 (3.53-fold). Enrichment factor at the 6th RoE is subject to complex population dynamics and propagation of sampling bias during the SEER workflow. For example, ZP_03935819.1, the most over-abundant recombinase in the starting pool (0.100) and the 3rd-most abundant recombinase at the end RoE (0.112), maintained its abundance through 6, suggesting that this candidate exhibited average performance within the context of the library. Enrichment factor is also presented after the 1st RoE (Table 3), which should be less subject to propagation biases, but more so to stochastic uncertainty. Here, only 16.6% of the pool expanded after one round, but eight candidates increased by at least 10-fold. However few persisted over all RoE. ZP_09377516.1 from Hafnia alvei, Q8KQW0 from Vibrio cholerae, & ZP_07797103.1 from Pseudomonas aeruginosa were advantageous. Two erf members, YP_001552302 from a Thalassaomonas phage, and YP_08900554.1 from an Enterobacter phage, start strong before declining in the face of many redβ competitors. These results offer a wealth of potential recombinases that could be leveraged in E. coli, but more importantly offer a representative workflow for recombinase discovery in non-standard model organisms.

Example IV
λ β Facilitates Annealing of Complementary SSB-Coated Oligonucleotides In Vitro

To understand more about the mechanisms of recombination, an in vitro oligo annealing assay was developed using two complementary 90mer oligos, one with a 3′-Fluorescein and the other with a 5′-Iowa Black FQ dark quencher (FIG. 5A). Upon mixing and incubating at 37° C., annealing reduces fluorescence over time. Annealing kinetics of the oligos is thus a platform with which the contributions of λ β or other SSAPs can be tested. To facilitate affinity purification, an N-terminal 6×His tag (SEQ ID NO: 108) was added and it was verified that the tag had no effect in its oligo recombination activity in E. coli (FIG. 9). Single stranded DNA binding proteins such as SSB protect ssDNA that is denatured during genome replication. Once bound, SSB inhibits complementary annealing {17272294}, until it is removed by the replisome or other interaction partners. Based on the functional analogy between λ β and RecO/Rad52 (which is a SSAP mediating annealing of complementary DNA strands and which is able to interact with the eukaryotic single-strand binding protein RPA) and the presence of SSB in some bacterial SSAP operons, the disclosure provides that an SSAP-SSB interaction represents a host-specific interaction node through which the SSAP interacts with the host system to facilitate recombination. An in vitro oligo quenching assay was set up using oligos pre-coated with SSB. In the absence of λ β, SSB-coated oligos support negligible annealing (FIG. 5C, purple curve). However, addition of λ β can overcome SSB pre-coating to facilitate annealing (FIG. 5B, 5C, red curve). Chelation of Mg²⁺ using EDTA significantly inhibits the annealing of SSB-coated oligos (FIG. 5B, blue curve), suggesting a Mg²⁺ requirement. To further characterize the λ β-SSB interaction, the effects of deleting eight C-terminal amino acids (DFDDDIPF (SEQ ID NO: 109)) of SSB (SSBAC8), previously shown to be dispensable for ssDNA binding but essential for its interaction with other proteins {8759000, 18937104}, and essential in vivo {8759000} was tested. λ β is not able to facilitate annealing of oligos that were pre-coated with SSBAC8 (FIG. 5B, green curve), suggesting that similar to other SSB-interacting proteins, λ β's interaction with SSB requires the SSB C-terminus. Compared to WT, the 1-194 truncation is unable to break the SSB inhibition, behaving very similarly to the SSB-only control (FIG. 5C, compare orange to purple curves). Similarly, K214A showed significantly decreased annealing of SSB-coated oligos (FIG. 5C, green curve).

Q8KQW0 is an advantageous SSAP. This Vibrio cholerae SSAP showed slightly reduced ssDNA binding affinity compared to λ β (FIG. 4D, purple squares), and was able to anneal oligos coated with E. coli SSB (FIG. 5C, blue curve). Taken together, these results provide that λ β interacts with SSB-coated ssDNA to facilitate annealing, and that the C-terminus of λ β plays a significant role in this proposed interaction and this SSB-SSAP interaction is important for oligo recombination in vivo.

Example V
The C-Terminal of λ β is Required Oligo Recombination

To further understand the host tropism SSAPs exhibit, the C-terminus of λ β was studied. First, the 266 amino acid protein was serially truncated, generating fragments 1-245, 1-228, 1-211, 1-194 and 1-177, which is the smallest fragment previously found to be sufficient for DNA binding (Wu et al., 2006). Whereas full-length λ β is capable of achieving oligo recombinations at high frequencies (0.0848 in FIG. 4A), even the smallest C-terminal truncation tested 031-245) decreased function at least ˜77-fold, and larger serial truncations exhibit similar loss-of-function phenotypes. To further interrogate the C-terminus of λ β, K, R, & F amino acids were mutated to alanine and these variants were tested in oligo recombination. These mutations were chosen because similar substitution of basic and aromatic amino acids ablate the RecO-SSB interaction (Ryzhikov et al., 2011). Some of the mutations severely reduced recombination frequencies, especially R192A, K214A, & F228A (FIG. 4B). A negative control mutant K172A, previously shown to abolish DNA binding (Wu et al., 2006), also had negligible recombination rates. Since the mutation screen revealed that single amino acid substitutions in the C-terminus of λ β had dramatic functional consequences for oligo recombination frequencies in vivo, a subset of these mutants was characterized in vitro via a gel shift assay (FIG. 4C, 4D). In this assay K214A was undistinguishable from WT λ β at binding a ssDNA 90-mer, while the 1-194 truncation had somewhat reduced affinity. These results suggest that the λ β C-terminal truncation and point mutation have preserved DNA binding but impaired in vivo recombination, suggesting the C-terminus has a function distinct from DNA binding that is required for oligo recombination.

Example V
Porting a Species-Matched Heterologous SSB Enables Gain of Recombinase Function in E. coli

The in vitro data of FIG. 5B-C provides that the species tropism seen in SSAPs is based on its ability to interact with SSB from ssDNA in a given model organism. To further evaluate this, foreign SSAPs were tested for a gain of function when they were co-expressed with a phylogenetically-matched SSB homolog. To test this in E. coli, an inducible, bi-cistronic vector was generated to express a candidate SSAP and either a matched or mismatched SSB. As candidates, SSAPs and SSBs were selected from E. coli, Lactobacillus reuteri {22328729}, and Corynebacterium glutamicum {23630315}, which are known to enable recombineering in their respective host organisms. To facilitate bi-cistronic expression of both ORFs in our pARC8-based vector, an RBS-containing motif (aaaataAGGAGGAaaaacat)(SEQ ID NO:10) was added downstream of the SSAP stop codon and upstream of an aadA coding region, which confers spectinomycin resistance. This plasmid conferred spectinomycin resistance in the presence of 0.2% L-arabinose, albeit supporting growth rates that were significantly slower than chromosomal, monocistronic expression of an aadA cassette.

pARC8 variants were constructed containing SSAPs only, properly matched SSAP-SSB pairs (e.g., λ β+EcSSB, Lr.recT1+LrSSB, or Cg.recT+CgSSB), or mismatched SSAP/SSB pairs (e.g., Lr.recT1+CgSSB, or Cg.recT+LrSSB). Induction was performed using 0.2% L-arabinose and oligo recombinations were performed to quantify AR Frequency (FIG. 6). Background (no plasmid) AR frequency was measured at 2.51E-5±1.48E-5 in E. coli, whereas λ β supported AR frequencies of 1.79E-1±1.29E-1 (mean±std. dev., **p<0.01, pARC8.λ β vs. background), consistent with a ˜1E4-fold increase, similar to that seen previously using λ Red in E. coli {11381128}. Bi-cistronic expression of E. coli λ β+EcSSB did not have a significant effect on fitness or on AR frequency (0.90E-1±0.68E-1, p=0.20, pARC8.λ β vs. pARC8.λ β_EcSSB). Although maximal over-expression of a heterologous SSB (LrSSB or CgSSB) may lead to toxicity, bi-cistronic overexpression using this system did not have any apparent effect on fitness in E. coli, suggesting that their expression is tolerated, at least at this level.

L. reuteri recT1 supported only a 15-fold increase in recombination rate over background in E. coli (3.13E-4±2.10E-4, p=ns pARC8.LrrecT1 vs background). However adding its cognate SSB further increased recombination frequency by ˜10-fold (3.00E-3±2.09E-3, *p<0.05 pARC8.LrrecT1_LrSSB vs pARC8.LrrecT1). An unrelated SSB (pARC8.LrrecT1_CgSSB) did not support this gain of function phenotype (4.96E-4±2.95E-4, p=ns, pARC8.LrrecT1_CgSSB vs pARc8.LrrecT1), nor did co-expressing E. coli SSB, suggesting that a functional relationship must be maintained between recombinase and SSB in the context of the host organism. This pattern was maintained with C. glutamicum recT. Although Cg.recT supported 1E3-fold increase in recombination rates over background (2.64E-2±1.18E-2), adding its cognate SSB increased AR frequencies another ˜10-fold to 2.45E-1±8.68E-2 (*p<0.05, pARC8.CgrecT_CgSSB vs pARC8.CgrecT), whereas an unrelated SSB (pARC8.CgrecT_LrSSB) does not support any gain-of-function (4.40E-2±2.40E-2, p=ns, pARC8.CgrecT_LrSSB vs pARC8.CgrecT).

The disclosure provides that oligo recombination via heterologous SSAPs is enhanced by expressing its corresponding SSB, further highlighting the importance of the SSAP-SSB interaction. The disclosure identifies useful SSAP candidates other than λ β. The disclosure provides that the C terminus of λ β facilitates recombination. The disclosure provides that proper function of the SSAP C-terminus is required for the λ β-SSB interaction. The disclosure provides that λ β-SSB interaction requires the extreme C-terminus of SSB implicating a protein-protein interaction.

The disclosure provides methods of recombineering or genome editing using an SSAP paired with its phylogenetically-matched SSB homolog in a foreign host cell. A cell is genetically modified to include a nucleic acid encoding the SSAP and a nucleic acid encoding the SSB. The nucleic acids are expressed by the cell. The SSAP and the SSB interact and a single stranded DNA is included in the genome of the cell. The disclosure provides that an SSAP-SSB pair is a minimally functional set required to port recombineering into non-standard model organisms.

Tables

TABLE 1

Exemplary Recombinases for Use in the Recombineering Methods Described herein.

Frequency of Recombinases in Library 2 throughout SEER Workflow. These data are

sorted by the frequency at 6 RoE from largest to smallest. This is a subset of

Table S1, including only the top 25 most abundant recombinases at the 6th RoE.

Frequency of Recombinase in Population at Indicated RoE

Accession ID
Clade
0
1
2
3
4
5
6

ZP_07797103.1
redB
0.006069
0.087155
0.252425
0.496366
0.201227
0.645734
0.556229

ZP_09377516.1
redB
0.001715
0.065338
0.148232
0.187097
0.075322
0.129997
0.291793

ZP_03935819.1
redB
0.099945
0.043402
0.085366
0.101573
0.04141
0.141074
0.112076

ZP_08900554.1
redB
0.004043
0.103575
0.147504
0.109183
0.016881
0.022072
0.014275

YP_001552302
erf
0.010264
0.147288
0.130424
0.047892
0.007072
0.011151
0.009077

Q8KQW0
redB
0.000247
0.004216
0.01591
0.011619
0.006185
0.011982
0.008571

NP_040617.1
redB
0.032941
0.016828
0.073765
0.010528
0.645134
0.033432
0.004992

ZP_04808991.1
redB
0.002255
0.004112
0.003903
0.001754
0.000846
0.000977
0.001477

Q9AKZ0
redB
0.000225
0.007636
0.01179
0.006744
0.001488
0.001833
0.000631

ZP_01947910.1
redB
0.002619
0.004029
0.004605
0.001445
0.001273
0.000281
0.000342

ZP_06691943.1
redB
0.013573
0.001012
0.000515
6.11E−05
3.19E−05
0.000203
0.000127

YP_001429745
redB
0.006977
0.00115
0.004247
0.000487
0.000224
0.000221
0.000108

ZP_07463816.1
redB
0.014396
0.048178
0.008293
0.003843
0.000267
0.000344
9.07E−05

NP_958297
redB
0.005724
0.000757
7.4E−05
3.05E−05
9.88E−06
7.44E−05
6.11E−05

ZP_08564335.1
redB
0.006915
0.095269
0.040859
0.005662
0.000433
5.43E−05
2.44E−05

YP_001285543
erf
0.004234
0.094347
0.013147
0.008766
0.000237
0.000193
1.22E−05

YP_001285915
erf
0.017154
0.028527
0.002657
0.00034
5E−05
1.21E−05
8.72E−06

YP_003006972.1
redB
0.000301
6.68E−05
2.96E−06
1.91E−06
1.98E−05
2.01E−06
5.23E−06

YP_004150652.1
redB
0.001105
0.000227
4.15E−05
0
6.54E−05
1E−05
5.23E−06

YP_112530
erf
0.010894
0.00159
0.000231
1.14E−05
5.49E−07
0
5.23E−06

YP_005892581.1
redB
0.000372
5.29E−05
0.000107
1.91E−06
8.68E−05
1.61E−05
5.23E−06

NP_958570
erf
0.006934
0.000561
9.77E−05
0
5.49E−07
2.01E−06
5.23E−06

Q7N2Y8
redB
0.000445
1.55E−06
8.88E−06
0
8.24E−06
0
5.23E−06

TABLE 2

Exemplary Recombinases for Use in the Recombineering Methods Described

herein. Enrichment Factor of Recombinases from Library 2. Here, enrichment factor

is calculated as freq_n/freq₀, where n is the RoE. These data are sorted

by the enrichment factor from the 6th RoE, from largest to smallest,

showing only recombinases where enrichment factor >1.

Enrichment Factor (count(n)/count(0) at Indicated n

Accession ID
Clade
0
1
2
3
4
5
6

ZP_09377516.1
redB
1
38.09
86.42
109.08
43.91
75.79
170.12

ZP_07797103.1
redB
1
14.36
41.59
81.79
33.16
106.40
91.65

Q8KQW0
redB
1
17.04
64.29
46.95
24.99
48.42
34.64

ZP_08900554.1
redB
1
25.62
36.48
27.00
4.18
5.46
3.53

Q9AKZ0
redB
1
33.91
52.35
29.94
6.61
8.14
2.80

ZP_03935819.1
redB
1
0.43
0.85
1.02
0.41
1.41
1.12

YP_001552302
erf
1
14.35
12.71
4.67
0.69
1.09
0.88

YP_003010343.1
redB
1
0
1.13
0.73
0.63
0
0.67

ZP_04808991.1
redB
1
1.82
1.73
0.78
0.38
0.43
0.66

NP_040617.1
redB
1
0.51
2.24
0.32
19.58
1.01
0.15

TABLE 3

Exemplary Recombinases for Use in the Recombineering Methods Described

herein. Enrichment Factor of Recombinases from Library 2. Here, enrichment factor

is calculated as freq_n/freq₀, where n is the RoE. These data are sorted

by the enrichment factor from the 1st RoE, from largest to smallest,

showing only recombinases where enrichment factor >1.

Enrichment Factor (freq(n)/freq(0) at Indicated n

Accession ID
Clade
0
1
2
3
4
5
6

ZP_09377516.1
redB
1
38.10
86.40
109.00
43.90
75.80
170.00

Q9AKZ0
redB
1
33.90
52.40
29.90
6.61
8.14
2.80

ZP_08900554.1
redB
1
25.60
36.50
27.00
4.18
5.46
3.53

YP_001285543
erf
1
22.30
3.10
2.07
0.06
0.05
0

Q8KQW0
redB
1
17.00
64.30
47.00
25.00
48.40
34.60

ZP_07797103.1
redB
1
14.40
41.60
81.80
33.20
106.00
91.70

YP_001552302
erf
1
14.40
12.70
4.67
0.69
1.09
0.88

ZP_08564335.1
redB
1
13.80
5.91
0.82
0.06
0.01
0

NP_815795.1
redB
1
4.83
2.77
0.22
0.04
0
0.01

YP_003150705.1
redB
1
3.71
1.89
0.18
0.01
0
0

ZP_07463816.1
redB
1
3.35
0.58
0.27
0.02
0.02
0.01

YP_004875641.1
redB
1
3.27
0.65
0.05
0
0
0

NP_463513.1
redB
1
2.51
0.50
0.01
0.01
0
0

ZP_03706978.1
redB
1
2.04
0.24
0.01
0
0
0

ZP_04808991.1
redB
1
1.82
1.73
0.78
0.38
0.43
0.66

YP_002233655.1
redB
1
1.72
0.34
0.02
0
0
0

YP_001285915
erf
1
1.66
0.16
0.02
0
0
0

NP_076707
redB
1
1.60
0.20
0.05
0
0
0

ZP_01947910.1
redB
1
1.54
1.76
0.55
0.49
0.11
0.13

YP_002995484.1
redB
1
1.48
0.11
0.01
0
0
0

YP_003084246.1
redB
1
1.46
0.28
0
0.05
0
0

YP_001293439
sak4
1
1.22
0.25
0.01
0
0
0

NP_455496.1
redB
1
1.21
0.27
0.02
0.13
0.01
0

YP_001409109.1
redB
1
1.19
2.26
0
10.90
1.54
0

YP_004479394.1
redB
1
1.03
0.75
0
0
0.04
0

TABLE 4

Recombinases.

RECOMBINASES

Saccharomyces cerevisiae

Homo sapiens

Saprospira grandis

Shewanella putrefaciens

Caldicellulosiruptor

Cyanophage pSS2

kristjanssonii

Anaeroce/lum
thermophilum

M yxococcus fulvus

Caldicellulosiruptor

hydrothermalis

Mycobacterium marinum

Photorhabdus luminescens

Myxococcus xanthus

Corallococcus coralloides

Persephone/la marina

Legionella pneumophila

Burkholderia pseudomallei

Stigmatella aurantiaca

Caldicellulosiruptor

saccharolyticus

Rhizobium loti

Vibrio cholerae

Listeria phage A118

Salmonella newport

Xylanimonas cellulosilytica

Caldice/lulosiruptor

kronotskyensis

Delftia sp.

Paracoccus denitrificans

Pelobacter propionicus

Psychrobacter sp.

Clostridium cellulovorans

Bifidobacterium longum subsp.

infantis

Sinorhizobium meliloti

Campylobacter curvus

Corynebacterium variabile

Xylella fastidiosa

Photorhabdus luminescens

Escherichia coli

subsp. laumondii

Lactotoccus phage u/16.2

Enterobacter sp.

Clostridium difficile

Paenibacillus sp.

A/lochromatium vinosum

Acidithiobacillus caldus

Vibrio splendidus

Carboxydothermus

Campylobacter jejuni subsp.

hydrogenoformans

doylei

Soda/is
glossinidius

Spirochaeta smaragdinae

Haemophilus parasuis serovar 5

Gluconobacter oxydans

Halanaerobium sp.

Enterobacter cloacae subsp.

cloacae

Neisseria meningitidis

Enterobacter aerogenes

Yersinia pseudotuberculosis

serogroup A

Bartone/la grahamii

Bartone/la tribocorum

Shewanelasp.

Proteus mirabilis

Streptococcus pneumoniae

Pseudomonas fluorescens

Vibrio cholerae

Alkaliphilus metalliredigens

Streptococcus pyogenes serotype

serotype 01

M12

Streptococcus pyogenes

Laribacter hongkongensis

Salmonella paratyphi B

serotype Ml

Salmonella heidelberg

Escherichia fergusonii

Hydrogenobacter thermophilus

Shigella dysenteriae serotype 1

Salmonella typhi

Aggregatibacter aphrophilus

Listeria innocua serovar 6a

Drosophila
melanogaster

Streptococcus pyogenes serotype

MS

Xenorhabdus bovienii

Streptococcus parauberis

Thermovibrio ammonificans

Streptococcus salivarius

Frateuria aurantia

Melissococcus plutonius

Avibacterium paragallinarum

Salmonella enterica subsp.

Haemophilus influenzae R3021

enterica serovar Typhi str. 404ty

Xylella fastidiosa Ann- 1

Bartone/la schoenbuchensis Rl

Enterobacteria phage lambda

Gluconacetobacter oboediens

Helicobacter cinaedi CCUG

Bartone/la sp. AR 15-3

174Bp2
18818

Listeria monocytogenes FSL

Listeria ivanovii FSL F6-596

Paenibacillus larvae subsp. larvae

R2-503

B-3650

Escherichia fergusonii ECD227
Enterobacteria phage Min27

Salmonella enterica subsp.

enterica serovar Rubislaw str.

A4-653

Myxococcus fulvus HW-1

Salmonella enterica subsp.

Salmonella enterica subsp.

enterica serovar Javiana str.

enterica serovar Senftenberg str.

GA_MM04042433
A4-543

Shigella dysenteriae

Salmonella enterica subsp.

Myxococcus xanthus DK 1622

enterica serovar Typhi str. AG3

Shigella dysenteriae 1617

Caldicellulosiruptor

Caldicellulosiruptor

hydrothermalis 108

kristjanssonii 177R1B

Corallococcus cora/loides DSM

Stigmatella aurantiaca DW4/3-1

Caldice/lulosiruptor

2259

saccharolyticus DSM 8903

Providencia rettgeri

Lactococcus phage phismq86

Caldicellulosiruptor

kronotskyensis 2002

Neisseria meningitidis WUE

Neisseria lactamica ¥92-1009

Burkholderia pseudomallei

2594

NCTC 13177

Clostridium botulinum C str.

Clostridium cellulovorans 7438

Xenorhabdus nematophila ATCC

Eklund

19061

aribacter hongkongensis

Burkholderia pseudomallei 668

Alkaliphilus metalliredigens

HLHK9

QYMF

Klebsiella pneumoniae

Vibrio cholerae 0139

Yersinia ruckeri

Haemophilus haemolyticus

Cyanophage PSS2

Providencia rettgeri DSM 1131

M21639

Lactococcus phage phiLC3

Frateuria aurantia DSM 6220

Haemophilus influenzae NT127

Streptococcus phage 2167

Aggregati bacter aphrophilus

Streptococcus pneumoniae EU-

NJ8700
NP01

Me/issococcus plutonius ATCC

Streptococcus infantis SK970

Salmonella enterica subsp.

35311

enterica serovar Montevideo str.

SARB30

Bacillus subtilis subsp.

Enterobacter cloacae subsp.

Photobacterium damselae subsp.

spizizenii TU-B-10

cloacae ATCC 13047

damselae

Neisseria bacilliformis ATCC

Clostridium botulinum C sir.

Enterobacter aerogenes KCTC

BAA-1200
Stockholm
2190

Pseudomonas aeruginosa 39016

Bartonella rochalimae ATCC

Fusobacterium sp. 3_1_5R

BAA-1498

Comamonas testosteroni S44

Pediococcus acidilactici DSM

Salmonella enterica subsp.

20284

houtenae str. ATCC BAA-1581

Sodalis glossinidius str.

Streptococcus parauberis KCTC

Bartonella tribocorum CIP

‘morsitans’
11537
105476

Klebsiella sp. 1_1_55

Lactobacillus ruminis SPM0211

Haemophilus

paraphrohaemolyticus HK411

Clostridium sporogenes PA

Mannheimia haemolytica

Klebsiella pneumoniae subsp.

3679
PHL213

rhinoscleromatis ATCC 13884

Streptococcus phage 8140

Sinorhizobium meliloti SMll

Streptococcus phage V22

Clostridium botulinum El str.

Streptococcus phage 9429.2

Spirochaeta smaragdinae DSM

‘Bo NT E Beluga’

11293

Sinorhizobium meliloti AK83

Streptococcus pyogenes str.

Carboxydothermus

Manfredo

hydrogenoformans Z-2901

Streptococcus pneumoniae

Klebsiella pneumoniae KCTC

Streptococcus salivarius JIM8777

GA11426
2242

Acinetobacter sp. SH024

Burkholderia sp. Chl-1

Streptococcus suis 89/1591

Mesorhizobium loti

Streptococcus suis SS12

listeria monocytogenes FSL Nl-

MAFF303099

017

lodobacteriophage phiPLPE

Bartonella grahamii as4aup

Fusobacterium sp. 12_1B

Cryptobacterium curtum DSM

Bacteroides caccae ATCC

Leptotrichia goodfellowii F0264

15641
43185

Listeria innocua Clip11262

Vibrio cholerae non-01/non-

Acinetobacter baumanniiAB900

0139

Halanaerobium

Mycobacterium marinum M

Peptoniphilus duerdenii ATCC

hydrogeniformans

BAA-1640

Serratia symbiotica str. Tucson

Bifidobacterium longum subsp.

Campylobacter jejuni subsp.

infantis 157F

doylei 269.97

Xylella fastidiosa subsp.

Xylella fastidiosa 9a5c

Acinetobacter sp. P8-3-8

fastidiosa GB514

Streptococcus phage M102

Campylobacter coli 1957

Corynebacterium variabile DSM

44702

Collinsella stercoris DSM

Campylobacter curvus 525.92

Campylobacter jejuni subsp.

13279

jejuni 2008-988

Campylobacter coli 67-8

Campylobacter coli LMG 9860

Campylobacter coli 2685

Campylobacter coli 132-6

Sebaldella termitidis ATCC

Xylanimonas cellulosilytica

33386
DSM 15894

Campylobacter coli 80352

Collinsella aerofaciens ATCC

Aggregatibacter segnis ATCC

25986
33393

Haemophilus parasuis SH0165

Pelobacter propionicus DSM

Haemophilus parasuis 29755

2379

Campylobacter upsaliensis

Acidithiobacillus caldus SM-1

Acidithiobacillus caldus ATCC

RM3195

51756

Streptococcus gallolyticus

Yersinia pseudotuberculosis IP

Burkholderia pseudomallei

subsp. gallolyticus TX20005
32953

Pasteur 52237

Sinorhizobium meliloti

Vibrio furnissii CIP 102972
Acetobacteraceae bacterium AT-

CCNWSX0020

5844

Helicobacter pullorum MIT 98-

Pseudomonas fluorescens

Photobacterium damselae subsp.

5489
SBW25

damselae CIP 102761

Sinorhizobium medicae

Hydrogenobacter thermophil us

Simkania negevensis z

WSM419
TK-6

Rhizobium leguminosarum bv.

Persephonella marina EX-Hl

Thiocapsa marina 5811

trifolii WSM2304

Thiorhodococcus drewsii AZl

Desuifitobacterium

Allochromatium vinosum DSM

metallireducens DSM 15288
180

Vibrio splendidus LGP32

Thermus aquaticus Y51MC23

Enterobacter sp. 638

Thiocystis violascens DSM 198

Vibrio cholerae 1587

Brevundimonas diminuta ATCC

11568

Delftia sp. Csl-4

Corynebacterium diphtheriae C7

Cronobacter phage ENT47670

(beta)

Thiorhodovibri o sp. 970

Paenibacillus sp. JDR-2

Photorhabdus luminescens subsp.

laumondii TTOl

Psychrobacter sp. PRwf-1

Aeromonas salmonicida subsp.

Gluconobacter oxydans 621H

salmonicida 01-6526

Sporosarcina newyorkensis

Acidovorax delafieldii 2AN

Paenibacillus elgii B69

2681

Methylomicrobium album BG8
Enterobacteriacea e bacterium

Paracoccus denitrificans PD1222

9_2_54FAA

Commensalibacter intestini

Mobiluncus curtisii ATCC

Listeria monocytogenes str. 1/2a

A911
51333
F6854

Pseudomonas syringae pv. pisi

Listeria monocytogenes FSL J2-

Pseudomonas syringae pv.

str. 17048
003

lachrymans str. M301315

Xanthobacter autotrophicus Py2

Ureaplasma parvum serovar 6

Rhodococcus phage REQ3

str. ATCC 27818

Ureaplasma urealyticum serovar

Ureaplasma urealyticum serovar

Halomonas elongata DSM 2581

8 str. ATCC 27618
12 str. ATCC 33696

Klebsiella oxytoca 10-5250

Paenibacillus larvae subsp.

Hafnia alvei ATCC 51873

larvae BRL-230010

Burkholderia cenocepacia J2315

Gardnerella vaginalis 409-05

Proteus penneri ATCC 35198

Acinetobacter baumannii ATCC

Staphylococcus aureus subsp.

Pseudoalteromonas sp. BSi20495

19606

aureus D139

Parabacteroides johnsoni i DSM

Citrobacter koseri ATCC BM-

Borrelia hermsii

18315
895

Salmonella enterica subsp.

Salmonella phage SPN1S

Salmonella enterica subsp.

enterica serovar Mississippi str.

enterica serovar Kentucky str.

A4-633

CDC 191

Shigella dysenteriae CDC 74-

Corynebacterium striatum

Klebsiella sp. MS 92-3

1112
ATCC 6940

Pantoea sp. aB

Proteus mirabilis ATCC 29906

Providencia alcalifaciens DSM

30120

Escherichia phage TL-2011b

Clostridium methylpentosum

Erwinia phage phiEt88

DSM 5476

Gemella sanguinis M325

Serratia odorifera DSM 4582

Pseudomonas brassicacearum

subsp. brassicacearum NFM421

Comamonas testosteroni KF-1

Edwardsiella tarda ATCC 23685

Brenneria sp. EniD312

Legionella pneumophila

Enterococcus faecalis TX0630

Edwardsiella ictaluri 93-146

Edwardsiella tarda EIB202

Acinetobacter radioresistens

Chelativorans sp. BNC1

SK82

Moraxella catarrhalis 101P30B1

Clostridium botulinum B str.

Xenorhabdus bovienii SS-2004

Eklund 178

Erwinia billingiae Eb661
EBPR podovirus 1

Methylobacterium nodulans ORS

2060

Pantoea vagans C9-1

Oceanicola sp. 5124

Salmonella enterica subsp.

enterica serovar Gaminara str.

A4-567

Klebsiella pneumoniae subsp.

Thermovibrio ammonificans

Corynebacterium glutamicum R

pneumoniae MGH 78578
HB-1

Phage Gifsy-1

Escherichia phage TL-2011c

Salmonella enterica subsp.

enterica serovar Typhimurium

Vibrio angustum S14

Corynebacterium ulcerans BR-

Listeria monocytogenes F5L F2-

AD22
515

Mycobacterium abscessus M93

Psychrobacter arcticus 273-4

Ahrensia sp. R2A130

Thermoanaerobacter italicus

Rhodomicrobium vannielii

Acinetobacter radioresistens

Ab9
ATCC 17100
SH164

Photobacterium profundum SS9

Segniliparus rotundus DSM

Megamonas funiformis YIT

44985
11815

Pseudomonas syringae pv.

Pseudomonas syringae pv.

Moraxella catarrhalis BCl

syringae B728a

maculicola str. ES4326

Eubacterium saburreum DSM

Clostridium hathewayi DSM

achnospiraceae bacterium

3986
13479
9_1_43BFAA

Eubacteriaceae bacterium

Burkholderia sp. CCGE1002

Bacteroides sp. 2_1_33B

ACC19a

Nitratifractor salsuginis DSM

Jonesia denitrificans DSM

Agrobacterium tumefaciens

16511
20603

Aeromonas hydrophila

butyrate-producing bacterium

Enterobacter hormaechei ATCC

553/4
49162

Pseudomonas aeruginosa

Pseudomonas phage phi297

Klebsiella variicola At-22

Burkholderia sp. TJl49

Klebsiella oxytoca 10-5243

Burkholderia phymatum 5TM815

Pseudomonas putida GB-1

Klebsiella pneumoniae 342

Shewanella putrefaciens 200

Saprospira grandis str. Lewin

Mycobacterium abscessus M94

Bradyrhizobium sp. STM 3843

Bacillus subtilis subsp. natto

Sodalis phage phiSG1

Parachlamydia acanthamoebae

BEST195

str. Hall's coccus

Achromobacter xylosoxidans

Desulfovibrio piger ATCC

Burkholderia dolosa AU0158

AXX-A
29098

delta proteobacterium NaphS2

Vibrio caribbenthicus ATCC

Dyadobacter fermentans DSM

BAA-2122
18053

Kordia algicida OT-1

Flavobacterium indicum

Vibrio harveyi 1DA3

GPTSAl00-9

Runella slithyformis DSM

Flavobacterium johnsoniae

Owenweeksia hongkongensis

19594
UW101
DSM 17368

Riemerella anatipestifer RA-GD

Flavobacteriales bacterium

Leadbetterella byssophila DSM

ALC-1
17132

Flavobacterium branchiophilum

Paenibacillus mucilaginosus

Pseudoalteromonas sp. BSi20439

Fl-15
K02

Burkholderia glumae BGRl

Weeksella virosa DSM 16922

Myroides odoratimimus CCUG

10230

Cyclobacterium marinum DSM

Lacinutrix sp. 5H-3-7-4

Bacteroides stercoris ATCC

745

43183

Pseudomonas fluorescens Fl13

Haliscomenobacter hydrossis

Pseudomonas putida BIRD-1

DSM 1100

Niastella koreensis GR20-10

Vibrio cholerae LMA3984-4

Muricauda ruestringensis DSM

13258

actococcus garvieae g2

Burkholderia sp. CCGE1003

Staphylococcus

pseudintermedius ED99

Flavobacterium columnare

Bacillus selenitireducens

5hewanella woodyi ATCC 51908

ATCC 49512
MLSlO

Lactobacillus rhamnosus GG

Pedobacter saltans DSM 12145

Flavobacterium psychrophilum

JIP02/86

Borrelia burgdorferi B31

Bacteriovorax marinus SJ

Burkholderia sp. 383

Fibrobacter succinogenes subsp.

Cupriavidus taiwanensis LMG

Pseudomonas fluorescens Pf0-1

succinogenes S85
19424

Pseudomonas mendocina NK-

Lactobacillus easel BD-11

Alcanivorax borkumensis SK2

01

Herminiimonas arsenicoxydans

Cellulophaga algicola DSM

Odoribacter laneus YIT 12061

14237

Lactococcus lactis subsp.

Cupriavidus metallidurans

Coprococcus eutactus ATCC

cremoris CNCM 1-1631
CH34
27759

Lactobacillus plantarum WCFSl

Bacteroides sp. 1_1_30

Clostridium acetobutylicum EA

2018

Lachnospiraceae bacterium

Streptococcus thermophilus

Clostridium sporogenes ATCC

ACC2
CNCM 1-1630
15579

Lactobacillus sakei subsp. sakei

Zobellia galactanivorans

Vibrio furnissii NCTC 11218

23K

Oceanimonas sp. GKl

Staphylococcus haemolyticus

Lactobacillus fermentum CECT

JCSC1435
5716

Halobacillus halophilus DSM

Chromohalobacter salexigens

Clostridium botulinum

2266
DSM 3043
BKT015925

Bordetella bronchiseptica RB50

Providencia stuartii MRSN 2154

Polynucleobacter necessarius

subsp. asymbioticus QLW-

PlDMWA-1

Tannerella sp.

Alistipes indistinctus YIT 12060

Pseudoalteromonas atlantica T6c

6_1_58FAA_CT1

Streptococcus mutans UA159

Aliivibrio salmonicida LF11238

Halomonas boliviensis LCl

gamma proteobacterium HdNl

Bacteroides dorei DSM 17855

Streptococcus intermedius F0395

Weissella koreensis KACC

Lactobacillus sp. 7_1_47FAA

Burkholderia sp. Yi23

15510

Shewanella amazonensis SB2B
Lachnospiraceae bacterium

Pseudomonas syringae pv.

2_1_46FM

phaseolicola 1448A

Pseudomonas aeruginosa M18

Streptococcus gallolyticus

Streptococcus pyogenes

subsp. gallolyticus ATCC 43143
MGAS1882

Fusobacterium nucleatum

Sphingobacterium sp. 21

Leptotrichia buccalis C-1013-b

subsp. animalis OT 420

Oceanobacillus iheyensis

Clostridium hathewayi WAL-

Clostridium sp. L2-SO

HTE831
18680

Lactobacillus johnsonii DPC

Streptococcus suis A7

Bordetella avium 197N

6026

Selenomonas noxia F0398

Psychromonas ingrahamii 37

Anaerostipes caccae DSM 14662

Clostridium sp. BNL1100

Coprococcus comes ATCC

Bordetella petrii DSM 12804

27758

Staphylococcus aureus subsp.

Lactobacillus salivarius CECT

Aeromonas hydrophila subsp.

aureus JKD6159
5713

hydrophila ATCC 7966

Tannerella forsythia ATCC

Desulfotomaculum ruminis

Dorea formicigenerans

43037
DSM 2154
4_6_53AFAA

Pseudomonas stutzeri DSM

Desulfosporosinus orientis DSM

Enterococcus faecalis 62

4166
765

Eubacterium eligens ATCC

Enterococcus saccharolyticus

Pseudogulbenkiania sp. NH8B

27750
30_1

Cronobacter sakazakii ATCC

Shewanella oneidensis MR-1

Selenomonas ruminantium subsp.

BAA-894

lactilytica TAM6421

Clostridium sp. SS2/1
Lachnospiraceae bacterium

Johnsonella ignava ATCC 51276

1_4_56FAA

Aeromonas salmonicida subsp.

Ralstonia solanacearum Po82

Dysgonomonas mossii DSM

salmonicida A449

22836

Parabacteroides merdae ATCC

Dysgonomonas gadei ATCC

Bacteroides coprocola DSM

43184
BAA-286
17136

Cellvibrio japonicus Ueda107
Lachnospiraceae bacterium

Spirochaeta africana DSM 8902

5_1_57FAA

Facklamia languida CCUG
gamma proteobacterium NORS-

Catonella morbi ATCC 51271

37842
3

Eubacterium ventriosum ATCC

Eikenella corrodens ATCC

Methylomonas methanica MC09

27560
23834

Citrobacter freundii

Blautia hydrogenotrophica DSM

Alteromonas macleodii str. ‘Deep

4_7_47CFAA
10507
ecotype’

Clostridium asparagiforme

Azotobacter vinelandii DJ
Lachnospiraceae bacterium

DSM 15981

7_1_58FAA

Paenibacillus sp. oral taxon 786

Alteromonas sp. SN2

Desulfitobacterium hafniense

str. D14

Y51

Geobacter metallireducens GS-
Lachnospiraceae bacterium

Methylomicrobium alcaliphilum

15
3_1_46FAA

Saccharophagus degradans 2-40

Melissococcus plutonius

Taylorella asinigenitalis MCE3

DAT561

Lactobacillus delbrueckii subsp.

Oribacterium sp. ACBl

Bacteroides pectinophilus ATCC

bulgaricus 2038

43243

Salmonella enterica subsp.
z23:-- str. RSK2980

Ruminococcus lactaris ATCC

arizonae serovar 62:z4

29176

Roseburia inulinivorans DSM

Neisseria flavescens

Paenibacillus terrae HPL-003

16841
NRL30031/H210

Shewanella piezotolerans WP3

Pediococcus claussenii ATCC

Chitinophaga pinensis DSM

BAA-344
2588

Gemella haemolysans M341

Shewanella sediminis HAW-

Shewanella loihica PV-4

EB3

Clostridium hylemonae DSM
Lachnospiraceae bacterium

Heliobacterium modesticaldumlcel

15053
4_1_37FAA

Geobacter sulfurreducens PCA

Bacteroides capillosus ATCC

Shewanella violacea DSS12

29799

Bacillus thuringiensis serovar

Shewanella baltica OS678
Erysipelotrichaceae bacterium

konkukian str. 97-27

21_3

Spirochaeta coccoides DSM

Bacillus pseudofirmus OF4

Shewanella baltica BA175

17374

Prevotella sp. oral taxon

Clostridium bolteae ATCC

Bacillus sp. B14905

302 str. F0323
BAA-613

Lachnospiraceae bacterium

Bacillus sp. JS

Abiotrophia defectiva ATCC

3_1_57FAA_CT1

49176

Ruminococcus sp.

Coprobacillus sp. 8_2_54BFAA

Shewanella putrefaciens CN-32

5_1_39B_FAA

Neisseria lactamica 020-06

Catenibacterium mitsuokai

Neisseria gonorrhoeae TCDC-

DSM 15897
NG08107

Lachnospiraceae bacterium

Bacillus licheniformis DSM 13 =

Clostridium sp. 7_3_54FAA

6_1_63FAA
ATCC 14580

Glaciecola nitratireducens

Listeria seeligeri serovar 1/2b

Escherichia phage phiVlO

FR1064
str. SLCC3954

Eubacterium sp. 3_1_31

Parvimonas micra ATCC 33270

Erysipelothrix rhusiopathiae str.

Fujisawa

Treponema pallidum subsp.

Clostridium nexile DSM 1787

Clostridium spiroforme DSM

pallidum DAL-1

1552

Pectobacterium atrosepticum

Sphaerochaeta globus str.

Rahnella aquatilis CIP 78.65 =

SCRl1043
Buddy
ATCC 33071

Clostridium kluyveri NBRC

Clostridium perfringens WAL-

Shuttleworthia satelles DSM

12016
14572
14600

Sphaerochaeta pleomorpha str.

Lactobacillus helveticus H10

Prevotella micans F0438

Grapes

Pantoea ananatis PA13

Pantoea ananatis LMG 20103

Leuconostoc mesenteroides

subsp. mesenteroides J18

Anaeromyxobacter sp. Fw109-5

Serratia sp. AS13

Prevotella maculosa OT 289

Acetobacterium woodii DSM

Roseburia hominis A2-183

Mycoplasma fermentans PG18

1030

Thiobacillus denitrificans

Yersinia enterocolitica subsp.

Desulfobacterium autotrophicum

ATCC 25259

palearctica Yl
HRM2

Sorangium cellulosum ‘So ce 56’

Subdoligranulum sp.

Prevotella histicola F0411

4_3_54A2FAA

Arcobacter butzleri ED-1

Eubacterium limosum KIST612

Arcobacter nitrofigilis DSM

7299

Leuconostoc sp. C2

Mycoplasma hyopneumoniae

Eubacterium haIIii DSM 3353

168

Cellulophaga lytica DSM 7489

Mycoplasma hyorhinis HUB-1

Mycoplasma hyorhinis MCLD

Helcococcus kunzii ATCC

Campylobacter showae

Granulicella mallensis

51366
RM3277
MP5ACTX8

Helicobacter pylori 83

Arcobacter sp. L

Gluconacetobacter xylinus

NBRC 3288

Anaerotruncus colihominis

Treponema brennaborense DSM

Myroides odoratimimus CIP

DSM 17241
12168
101113

Granulibacter bethesdensis

Bacteroides sp. 2_2_4

Campylobacter jejuni

CGDNIHl

Bacteroides sp. D20

Geobacillus thermodenitrificans

Bacteroides sp. 9_1_42FAA

NG80-2

Microcystis aeruginosa PCC

Bacteroides fragilis 3_1_12

lmtechella halotolerans Kl

7806

Acaryochloris marina

Caldilinea aerophila DSM

Planctomyces brasiliensis DSM

MBIC11017
14535 = NBRC 104270
5305

Bacteroides sp. 01

Trichodesmium erythraeum

Homo sapiens

IMS101 Saccharomyces cerevisiae

Saprospiragrandis

Shewanella putrefaciens

Caldicellulosiruptor kristjanssonii

Cyanophage pSS2

Anaeroce/lum thermophilum

Myxococcus fulvus

Caldicellulosiruptor

Mycobacterium marinum

Photorhabdus luminescens

hydrothermalis

Myxococcus xanthus

Corallococcus coralloides

Persephone/la marina

Legionella pneumophila

Burkholderia pseudomallei

Stigmatella aurantiaca

Caldicellulosiruptor

Rhizobium loti

Vibrio cholerae

saccharolyticus

Listeria phage A118

Salmonella newport

Xylanimonas cellulosilytica

Caldice/lulosiruptor

Delftia sp.

Paracoccus denitrificans

kronotskyensis

Pelobacter propionicus

Psychrobacter sp.

Clostridium cellulovorans

Bifidobacterium longum subsp.

Sinorhizobium meliloti

Campylobacter curvus

infantis

Corynebacterium variabile

Xylella fastidiosa

Photorhabdus luminescens subsp.

laumondii

Escherichia coli

Lactotoccus phage u/l6.2

Enterobacter sp.

Clostridium difficile

Paenibacillus sp.

A/lochromatium vinosum

Acidithiobacillus caldus

Vibrio splendidus

Carboxydothermus

hydrogenoformans

Campylobacter jejuni subsp.

Soda/is glossinidius

Spirochaeta smaragdinae

doylei

Haemophilus parasuis serovar 5

Gluconobacter oxydans

Halanaerobium sp.

Enterobacter cloacae subsp.

Neisseria meningitidis serogroup

Enterobacter aerogenes

cloacae

A

Yersinia pseudotuberculosis

Bartone/la grahamii

Bartone/la tribocorum

Shewane/la sp.

Proteus mirabilis

Streptococcus pneumoniae

Pseudomonas fluorescens

Vibrio cholerae serotype O1

Alkaliphilus metalliredigens

Streptococcus pyogenes

Streptococcus pyogenes

Laribacter hongkongensis

serotype M12
serotype Ml

Salmonella paratyphi B

Salmonella heidelberg

Escherichia fergusonii

Hydrogenobacter thermophilus

Shigella dysenteriae serotype 1

Salmonella typhi

Aggregatibacter aphrophilus

Listeria innocua serovar 6a

Drosophila melanogaster

Streptococcus pyogenes

Xenorhabdus bovienii

Streptococcus parauberis

serotype MS

Thermovibrio ammonificans

Streptococcus salivarius

Frateuria aurantia

Melissococcus plutonius

Avibacterium paragallinarum

Salmonella enterica subsp.

enterica serovar Typhi str. 404ty

Haemophilus influenzae R3021

Xylella fastidiosa Ann- 1

Bartone/la schoenbuchensis R1

Enterobacteria phage lambda

Gluconacetobacter oboediens

Helicobacter cinaedi CCUG

174Bp2
18818

Bartone/la sp. AR 15-3

Listeria monocytogenes FSL

Listeria ivanovii FSLF6-596

R2-503

Paenibacillus larvae subsp. larvae

Escherichia fergusonii ECD227
Enterobacteria phage Min27

B-3650

Salmonella enterica subsp.

Myxococcus fulvus HW-1

Salmonella enterica subsp.

enterica serovar Rubislaw str.

enterica serovar Javiana str.

A4-653

GA_MM04042433

Salmonella enterica subsp.

Shigella dysenteriae

Salmonella enterica subsp.

enterica serovar Senftenberg str.

enterica serovar Typhi str. AG3

A4-543

Myxococcus xanthus DK 1622

Shigella dysenteriae 1617

Caldicellulosiruptor

hydrothermalis 108

Caldicellulosiruptor

Corallococcus cora/loides DSM

Stigmatella aurantiaca DW4/3-1

kristjanssonii 177R1B
2259

Caldice/lulosiruptor

Providencia rettgeri

Lactococcus phage phismq86

saccharolyticus DSM 8903

Caldicellulosiruptor

Neisseria meningitidis WUE

Neisseria lactamica ¥92-1009

kronotskyensis 2002
2594

Burkholderia pseudomallei

Clostridium botulinum C str.

Clostridium cellulovorans 7438

NCTC 13177
Eklund

Xenorhabdus nematophila

aribacter hongkongensis

Burkholderia pseudomallei 668

ATCC l9061
HLHK9

Alkaliphilus metalliredigens

Klebsiella pneumoniae

Vibrio cholerae 0139

QYMF

Yersinia ruckeri

Haemophilus haemolyticus

Cyanophage PSS2

M21639

Providencia rettgeri DSM 1131

Lactococcus phage phiLC3

Frateuria aurantia DSM 6220

Haemophilus influenzae NT127

Streptococcus phage 2167

Aggregatibacter aphrophilus

NJ8700

Streptococcus pneumoniae EU-

Me/issococcus plutonius ATCC

Streptococcus infantis SK970

NP01
35311

Salmonella enterica subsp.

Bacillus subtilis subsp. spizizenii

Enterobacter cloacae subsp.

enterica serovar Montevideo str.
TU-B-10

cloacae ATCC 13047

SARB30

Photobacterium damselae subsp.

Neisseria bacilliformis ATCC

Clostridium botulinum C sir.

damselae

BAA-1200
Stockholm

Enterobacter aerogenes KCTC

Pseudomonas aeruginosa 39016

Bartonella rochalimae ATCC

2190

BAA-1498

Fusobacterium sp. 3_1_5R

Comamonas testosteroni S44

Pediococcus acidilactici DSM

20284

Salmonella enterica subsp.

Sodalis glossinidius str.

Streptococcus parauberis KCTC

houtenae str. ATCC BAA-1581
‘morsitans’
11537

Bartonella tribocorum CIP

Klebsiella sp. 1_1_55

Lactobacillus ruminis SPM0211

105476

Haemophilus

Clostridium sporogenes PA

Mannheimia haemolytica

paraphrohaemolyticus HK411
3679
PHL213

Klebsiella pneumoniae subsp.

Streptococcus phage 8140

Sinorhizobium meliloti SMll

rhinoscleromatis ATCC 13884

Streptococcus phage V22

Clostridium botulinum El str.

Streptococcus phage 9429.2

‘BoNT E Beluga’

Spirochaeta smaragdinae DSM

Sinorhizobium meliloti AK83

Streptococcus pyogenes str.

11293

Manfredo

Carboxydothermus

Streptococcus pneumoniae

Klebsiella pneumoniae KCTC

hydrogenoformans Z-2901
GA11426
2242

Streptococcus salivarius

Acinetobacter sp. SH024

Burkholderia sp. Chl-1

JIM8777

Streptococcus suis 89/1591

Mesorhizobium loti

Streptococcus suis SS12

MAFF303099

listeriamonocytogenes FSL Nl-

lodobacteriophage phiPLPE

Bartonella grahamii as4aup

017

Fusobacterium sp. 12_1B

Cryptobacterium curtum DSM

Bacteroides caccae ATCC 43185

l5641

Leptotrichia goodfellowii

Listeria innocua Clip11262
Vibrio cholerae non-01/non-

F0264

0139

Acinetobacter

Halanaerobium

Mycobacterium marinum M

baumanniiAB900

hydrogeniformans

Peptoniphilus duerdenii ATCC

Serratia symbiotica str. Tucson

Bifidobacterium longum subsp.

BAA-1640

infantis 157F

Campylobacter jejuni subsp.

Xylella fastidiosa subsp.

Xylella fastidiosa 9a5c

doylei269.97

fastidiosa GB514

Acinetobacter sp. P8-3-8

Streptococcus phage M102

Campylobacter coli 1957

Corynebacterium variabile

Collinsella stercoris DSM

Campylobacter curvus 525.92

DSM44702
13279

Campylobacter jejuni subsp.

Campylobacter coli67-8

Campylobacter coli LMG 9860

jejuni 2008-988

Campylobacter coli 2685

Campylobacter coli 132-6

Sebaldella termitidis ATCC

33386

Xylanimonas cellulosilytica

Campylobacter coli80352

Collinsella aerofaciens ATCC

DSM 15894

25986

Aggregatibacter segnis ATCC

Haemophilus parasuis SH0165

Pelobacter propionicus DSM

33393

2379

Haemophilus parasuis 29755

Campylobacter upsaliensis

Acidithiobacillus caldus SM-1

RM3195

Acidithiobacillus caldus ATCC

Streptococcus gallolyticus

Yersinia pseudotuberculosis IP

51756
subsp. gallolyticus TX20005
32953

Burkholderia pseudomallei

Sinorhizobium meliloti

Vibrio furnissii CIP 102972

Pasteur 52237
CCNWSX0020

Acetobacteraceae bacterium

Helicobacter pullorum MIT 98-

Pseudomonas fluorescens

AT-5844
5489
SBW25

Photobacterium damselae subsp.

Sinorhizobium medicae

Hydrogenobacter thermophilus

damselae CIP 102761
WSM419
TK-6

Simkania negevensis z

Rhizobium leguminosarum bv.

Persephonella marina EX-Hl

trifolii WSM2304

Thiocapsa marina 5811

Thiorhodococcus drewsii AZl

Desuifitobacterium

metallireducens DSM 15288

Allochromatium vinosum DSM

Vibrio splendidus LGP32

Thermus aquaticus Y51MC23

180

Enterobacter sp. 638

Thiocystis violascens DSM 198

Vibrio cholerae 1587

Brevundimonas diminuta ATCC

Delftia sp. Csl-4

Corynebacterium diphtheriae C7

11568

(beta)

Cronobacter phage ENT47670

Thiorhodovibrio sp. 970

Paenibacillus sp. JDR-2

Photorhabdus luminescens

Psychrobacter sp. PRwf-1

Aeromonas salmonicida subsp.

subsp. laumondii TTOl

salmonicida 01-6526

Gluconobacter oxydans 621H

Sporosarcina newyorkensis

Acidovorax delafieldii 2AN

2681

Paenibacillus elgii B69

Methylomicrobium album BG8
Enterobacteriaceae bacterium

9_2_54FAA

Paracoccus denitrificans

Commensalibacter intestini

Mobiluncus curtisii ATCC 51333

PD1222
A911

Listeria monocytogenes str. 1/2a

Pseudomonas syringae pv. pisi

Listeria monocytogenes FSLJ2-

F6854
str. l7048
003

Pseudomonas syringae pv.

Xanthobacter autotrophicus Py2

Ureaplasma parvum serovar 6 str.

lachrymans str. M301315

ATCC 27818

Rhodococcus phage REQ3

Ureaplasma urealyticum serovar

Ureaplasma urealyticum serovar

8 str. ATCC 27618
l2 str. ATCC 33696

Halomonas elongata DSM 2581

Klebsiella oxytoca 10-5250

Paenibacillus larvae subsp. larvae

BRL-230010

Hafnia alvei ATCC 51873

Burkholderia cenocepacia J2315

Gardnerella vaginalis 409-05

Proteus penneri ATCC 35198

Acinetobacter baumanniiATCC

Staphylococcus aureus subsp.

19606

aureus D139

Pseudoalteromonas sp.

Parabacteroides johnsoni i DSM

Citrobacter koseri ATCC BM-

BSi20495
18315
895

Borrelia hermsii

Salmonella enterica subsp.

Salmonella phage SPN1S

enterica serovar Mississippi str.

A4-633

Salmonella enterica subsp.

Shigella dysenteriae CDC 74-

Corynebacterium striatum ATCC

enterica serovar Kentucky str.
11l2
6940

CDC 191

Klebsiella sp. MS 92-3

Pantoea sp. aB

Proteus mirabilis ATCC 29906

Providencia alcalifaciens DSM

Escherichia phage TL-2011b

Clostridium methylpentosum

30120

DSM 5476

Erwinia phage phiEt88

Gemella sanguinis M325

Serratia odorifera DSM 4582

Pseudomonas brassicacearum

Comamonas testosteroni KF-1

Edwardsiella tarda ATCC 23685

subsp. brassicacearum NFM421

Brenneria sp. EniD312

Legionella pneumophila

Enterococcus faecalis TX0630

Edwardsiella ictaluri 93-146

Edwardsiella tarda EIB202

Acinetobacter radioresistens

SK82

Chelativorans sp. BNC1

Moraxella catarrhalis 101P30B1

Clostridium botulinum B str.

Eklund 178

Xenorhabdus bovienii SS-2004

Erwinia billingiae Eb661
EBPR podovirus 1

Methylobacterium nodulans

Pantoea vagans C9-1

Oceanicola sp. 5124

ORS 2060

Salmonella enterica subsp.

Klebsiella pneumoniae subsp.

Thermovibrio ammonificans

enterica serovar Gaminara str.

pneumoniae MGH 78578
HB-1

A4-567

Corynebacterium glutamicum R
Phage Gifsy-1

Escherichia phage TL-2011c

Salmonella enterica subsp.

Vibrio angustum S14

Corynebacterium ulcerans BR-

enterica serovar Typhimurium

AD22

Listeria monocytogenes F5L F2-

Mycobacterium abscessus M93

Psychrobacter arcticus 273-4

515

Ahrensia sp. R2A 130

Thermoanaerobacter italicus

Rhodomicrobium vannielii

Ab9
ATCC 17100

Acinetobacter radioresistens

Photobacterium profundum SS9

Segniliparus rotundus DSM

SH164

44985

Megamonas funiformis YIT

Pseudomonas syringae pv.

Pseudomonas syringae pv.

11815

syringae B728a

maculicola str. ES4326

Moraxella catarrhalis BCl

Eubacterium saburreum DSM

Clostridium hathewayi DSM

3986
13479

achnospiraceae bacterium
Eubacteriaceae bacterium

Burkholderia sp. CCGE1002

9_1_43BFAA
ACC19a

Bacteroides sp. 2_1_33B

Nitratifractor salsuginis DSM

Jonesia denitrificans DSM 20603

16511

Agrobacterium tumefaciens

Aeromonas hydrophila

butyrate-producing bacterium

553/4

Enterobacter hormaechei ATCC

Pseudomonas aeruginosa

Pseudomonas phage phi297

49162

Klebsiella variicola At-22

Burkholderia sp. TJ149

Klebsiella oxytoca 10-5243

Burkholderia phymatum

Pseudomonas putida GB-1

Klebsiella pneumoniae 342

5TM815

Shewanella putrefaciens 200

Saprospira grandis str. Lewin

Mycobacterium abscessus M94

Bradyrhizobium sp. STM 3843

Bacillus subtilis subsp. natto

Sodalis phage phiSG1

BEST195

Parachlamydia acanthamoebae

Achromobacter xylosoxidans

Desulfovibrio piger ATCC 29098

str. Hall's coccus
AXX-A

Burkholderia dolosa AU0158
delta proteobacterium NaphS2

Vibrio caribbenthicus ATCC

BAA-2122

Dyadobacter fermentans DSM

Kordia algicida OT-1

Flavobacterium indicum

18053

GPTSAl00-9

Vibrio harveyi 1DA3

Runella slithyformis DSM

Flavobacterium johnsoniae

19594
UW101

Owenweeksia hongkongensis

Riemerella anatipestifer RA-GD

Flavobacteriales bacterium ALC-

DSM 17368

1

Leadbetterella byssophila DSM

Flavobacterium branchiophilum

Paenibacillus mucilaginosus K02

17132
Fl-15

Pseudoalteromonas sp.

Burkholderia glumae BGRl

Weeksella virosa DSM 16922

BSi20439

Myroides odoratimimus CCUG

Cyclobacterium marinum DSM

Lacinutrix sp. 5H-3-7-4

10230
745

Bacteroides stercoris ATCC

Pseudomonas fluorescens Fl13

Haliscomenobacter hydrossis

43183

DSM 1100

Pseudomonas putida BIRD-1

Niastella koreensis GR20-10

Vibrio cholerae LMA3984-4

Muricauda ruestringensis DSM

actococcus garvieae g2

Burkholderia sp. CCGE1003

13258

Staphylococcus

Flavobacterium columnare

Bacillus selenitireducens MLSlO

pseudintermedius ED99
ATCC 49512

5hewanella woodyi ATCC

Lactobacillus rhamnosus GG

Pedobacter saltans DSM 12145

51908

Flavobacterium psychrophilum

Borrelia burgdorferi B31

Bacteriovorax marinus SJ

JIP02/86

Burkholderia sp. 383

Fibrobacter succinogenes subsp.

Cupriavidus taiwanensis LMG

succinogenes S85
19424

Pseudomonas fluorescens Pf0-1

Pseudomonas mendocina NK-01

Lactobacillus easel BD-11

Alcanivorax borkumensis SK2

Herminiimonas arsenicoxydans

Cellulophaga algicola DSM

14237

Odoribacter laneus YIT 12061

Lactococcus lactis subsp.

Cupriavidus metallidurans CH34

cremoris CNCM 1-1631

Coprococcus eutactus ATCC

Lactobacillus plantarum WCFSl

Bacteroides sp. 1_1_30

27759

Clostridium acetobutylicum EA
Lachnospiraceae bacterium

Streptococcus thermophilus

2018
ACC2
CNCM 1-1630

Clostridium sporogenes ATCC

Lactobacillus sakei subsp. sakei

Zobellia galactanivorans

15579
23K

Vibrio furnissii NCTC 11218

Oceanimonas sp. GK1

Staphylococcus haemolyticus

JCSC1435

Lactobacillus fermentum CECT

Halobacillus halophilus DSM

Chromohalobacter salexigens

5716
2266
DSM 3043

Clostridium botulinum

Bordetella bronchiseptica RB50

Providencia stuartii MRSN 2154

BKT015925

Polynucleobacter necessarius

Tannerella sp. 6_1_58FAA_CT1

Alistipes indistinctus YIT 12060

subsp. asymbioticus QLW-

PlDMWA-1

Pseudoalteromonas atlantica

Streptococcus mutans UA159

Aliivibrio salmonicida LF11238

T6c

Halomonas boliviensis LCl
gamma proteobacterium HdNl

Bacteroides dorei DSM 17855

Streptococcus intermedius

Weissella koreensis KACC

Lactobacillus sp. 7_1_47FAA

F0395
15510

Burkholderia sp. Yi23

Shewanella amazonensis SB2B
Lachnospiraceae bacterium

2_1_46FM

Pseudomonas syringae pv.

Pseudomonas aeruginosa M18

Streptococcus gallolyticus subsp.

phaseolicola 1448A

gallolyticus ATCC 43143

Streptococcus pyogenes

Fusobacterium nucleatum

Sphingobacterium sp. 21

MGAS1882
subsp. animalis OT 420

Leptotrichia buccalis C-1013-b

Oceanobacillus iheyensis

Clostridium hathewayi WAL-

HTE831
18680

Clostridium sp. L2-SO

Lactobacillus johnsonii DPC

Streptococcus suis A7

6026

Bordetella avium 197N

Selenomonas noxia F0398

Psychromonas ingrahamii 37

Anaerostipes caccae DSM 14662

Clostridium sp. BNL1100

Coprococcus comes ATCC 27758

Bordetella petrii DSM 12804

Staphylococcus aureus subsp.

Lactobacillus salivarius CECT

aureus JKD6159
5713

Aeromonas hydrophila subsp.

Tannerella forsythia ATCC

Desulfotomaculum ruminis DSM

hydrophila ATCC 7966
43037
2154

Dorea formicigenerans

Pseudomonas stutzeri DSM 4166

Desulfosporosinus orientis DSM

4_6_53AFAA

765

Enterococcus faecalis 62

Eubacterium eligens ATCC

Enterococcus saccharolyticus

27750
30_1

Pseudogulbenkiania sp. NH8B

Cronobacter sakazakii ATCC

Shewanella oneidensis MR-1

BAA-894

Selenomonas ruminantium

Clostridium sp. SS2/1
Lachnospiraceae bacterium

subsp. lactilytica TAM6421

1_4_56FAA

Johnsonella ignava ATCC 51276

Aeromonas salmonicida subsp.

Ralstonia solanacearum Po82

salmonicida A449

Dysgonomonas mossii DSM

Parabacteroides merdae ATCC

Dysgonomonas gadei ATCC

22836
43184
BAA-286

Bacteroides coprocola DSM

Cellvibrio japonicus Ueda107
Lachnospiraceae bacterium

17136

5_1_57FAA

Spirochaeta africana DSM 8902

Facklamia languida CCUG
gamma proteobacterium NORS-3

37842

Catonella morbi ATCC 51271

Eubacterium ventriosum ATCC

Eikenella corrodens ATCC 23834

27560

Methylomonas methanica

Citrobacter freundii

Blautiahydrogenotrophica DSM

MC09
4_7_47CFAA
10507

Alteromonas macleodii str.

Clostridium asparagiforme

Azotobacter vinelandii DJ

‘Deep ecotype’
DSM 15981

Lachnospiraceae bacterium

Paenibacillus sp. oral taxon 786

Alteromonas sp. SN2

7_1_58FAA
str. D14

Desulfitobacterium hafniense

Geobacter metallireducens GS-
Lachnospiraceae bacterium 3_1_46FAA

Y51
15

Methylomicrobium alcaliphilum

Saccharophagus degradans 2-40

Melissococcus plutonius

DAT561

Taylorella asinigenitalis MCE3

Lactobacillus delbrueckii subsp.

Oribacterium sp. ACB1

bulgaricus 2038

Bacteroides pectinophilus ATCC

Salmonella enterica subsp.
z23:-- str. RSK2980

43243

arizonae serovar 62:z4

Ruminococcus lactaris ATCC

Roseburia inulinivorans DSM

Neisseria flavescens

29176
16841
NRL30031/H210

Paenibacillus terrae HPL-003

Shewanella piezotolerans WP3

Pediococcus claussenii ATCC

BAA-344

Chitinophaga pinensis DSM

Gemella haemolysans M341

Shewanella sediminis HAW-EB3

2588

Shewanella loihica PV-4

Clostridium hylemonae DSM
Lachnospiraceae bacterium

15053
4_1_37FAA

Heliobacterium modesticaldum

Geobacter sulfurreducens PCA

Bacteroides capillosus ATCC

1cel

29799

Shewanella violacea DSS12

Bacillus thuringiensis serovar

Shewanella baltica OS678

konkukian str. 97-27

Erysipelotrichaceae bacterium

Spirochaeta coccoides DSM

Bacillus pseudofirmus OF4

21_3
17374

Shewanella baltica BA175

Prevotella sp. oral taxon 302 str.

Clostridium bolteae ATCC BAA-

F0323
613

Bacillus sp. B14905
Lachnospiraceae bacterium

Bacillus sp. JS

3_1_57FAA_CT1

Abiotrophia defectiva ATCC

Ruminococcus sp. 5_1_39B_FAA

Coprobacillus sp. 8_2_54BFAA

49176

Shewanella putrefaciens CN-32

Neisseria lactamica 020-06

Catenibacterium mitsuokai DSM

15897

Neisseria gonorrhoeae TCDC-
Lachnospiraceae bacterium

Bacillus licheniformis DSM 13 =

NG08107
6_1_63FAA
ATCC 14580

Clostridium sp. 7_3_54FAA

Glaciecola nitratireducens

Listeria seeligeri serovar 1/2b str.

FR1064
SLCC3954

Escherichia phage phiVlO

Eubacterium sp. 3_1_31

Parvimonas micra ATCC 33270

Erysipelothrix rhusiopathiae str.

Treponema pallidum subsp.

Clostridium nexile DSM 1787

Fujisawa

pallidum DAL-1

Clostridium spiroforme DSM

Pectobacterium atrosepticum

Sphaerochaeta globus str. Buddy

1552
SCR11043

Rahnella aquatilis CIP 78.65 =

Clostridium kluyveri NBRC

Clostridium perfringens WAL-

ATCC 33071
12016
14572

Shuttleworthia satelles DSM

Sphaerochaeta pleomorpha str.

Lactobacillus helveticus H10

14600
Grapes

Prevotella micans F0438

Pantoea ananatis PA13

Pantoea ananatis LMG 20103

Leuconostoc mesenteroides

Anaeromyxobacter sp. Fw109-5

Serratia sp. AS13

subsp. mesenteroides J18

Prevotella maculosa OT 289

Acetobacterium woodii DSM

Roseburia hominis A2-183

1030

Mycoplasma fermentans PG18

Thiobacillus denitrificans

Yersinia enterocolitica subsp.

ATCC 25259

palearctica Yl

Desulfobacterium

Sorangium cellulosum ‘So ce 56’

Subdoligranulum sp.

autotrophicum HRM2

4_3_54A2FAA

Prevotella histicola F0411

Arcobacter butzleri ED-1

Eubacterium limosum KIST612

Arcobacter nitrofigilis DSM

Leuconostoc sp. C2

Mycoplasma hyopneumoniae 168

7299

Eubacterium haIIii DSM 3353

Cellulophaga lytica DSM 7489

Mycoplasma hyorhinis HUB-1

Mycoplasma hyorhinis MCLD

Helcococcus kunzii ATCC

Campylobacter showae RM3277

51366

Granulicella mallensis

Helicobacter pylori 83

Arcobacter sp. L

MP5ACTX8

Gluconacetobacter xylinus

Anaerotruncus colihominis

Treponema brennaborense DSM

NBRC 3288
DSM 17241
12168

Myroides odoratimimus CIP

Granulibacter bethesdensis

Bacteroides sp. 2_2_4

101113
CGDNIHl

Campylobacter jejuni

Bacteroides sp. D20

Geobacillus thermodenitrificans

NG80-2

Bacteroides sp. 9_1_42FAA

Microcystis aeruginosa PCC

Bacteroides fragilis 3_1_12

7806

lmtechella halotolerans K1

Acaryochloris marina

Caldilinea aerophila DSM 14535 =

MBIC11017
NBRC 104270

Planctomyces brasiliensis DSM

Bacteroides sp. 01

Trichodesmium erythraeum

5305

IMS101

TABLE 5

Recombinases.

RECOMBINASES

YML032

Saccharomyces cerevisiae

P433S1

Homo sapiens

H6L1F4

Saprospira grandis

E6XGY4

Shewanella putrefaciens

E4SSA7

Caldicellulosiruptor kristjanssonli

C7F4E8
Cyanophage pSS2

B9MNF3

Anaerocellum thermophilum

F8CQD8

Myxococcus fulvus

E4QCOO

Caldicellulosi ruptor hydrothermalis

B2HP13

Mycobacterium marinum

Q7N2Y8

Photorhabdus luminescens

Q1DAT2

Myxococcus xanthus

H8MHN3

Corallococcus coralloides

COQSA2

Persephonella marina

Q9AKZO

Legionella pneumophila

A3NMOO

Burkholderia pseudomallei

Q08VK7

Stigmatella aurantiaca

A4XJGS

Caldicellulosiruptor saccharolyticus

Q984J6

Rhizobium loti

Q8KQWO

Vibrio cholerae

Q9T172

Listeria phage A118

A41VH9

Salmonella newport

D1BWP7

Xylanlmonas cellulosilytica

E4SEV3

Caldicellulosiruptor kronotskyensis

F6AU64

Delftia sp.

A1B8G1

Paracoccus denltrificans

A1AQ73

Pelobacter propionicus

ASWFS1

Psychrobacter sp.

D9SWGO

Clostridlum cellulovorans

E8MTD2

Bifidobacterium longum subsp. infantis

F6DWY3

Sinorhizobium melilotl

A7HOW7

Campylobacter curvus

GOHBS6

Corynebacterium variabile

Q87CQ1

Xylella fastidiosa

Q7N2Y8

Photorhabdus luminescens subsp. laumondii

BlXAU6

Escherichia coli

Q9MBV8

Lactotoccus phage ull6.2

A4W7K6

Enterobacter sp.

Q18AA7

Clostridium difficile

C6CX43

Paenibacillus sp.

D3RWF4

Allochromatium vinosum

F9ZNV9

Acidithiobacillus caldus

B7VNT1

Vibrio splendidus

Q3ABHS

Carboxydothermus hydrogenoformans

A7H394

Campylobacter jejuni subsp. doylei

Q2NSA3

Sodalis glossinidius

ElR1F4

Spirochaeta smaragdinae

B8F617

Haemophilus parasuis serovar S

QSFN39

Gluconobacter oxydans

E4RNA4

Halanaerobium sp.

DSCJK9

Enterobacter cloacae subsp. cloacae

E7BFDS

Neisseria meningitidis serogroup A

GOEOR4

Enterobacter aerogenes

Q66Bl7

Yersinia pseudotuberculosis

C6ACZ8

Bartonella grahamii

A91YA2

Bartone/la tribocorum

A1RH51

Shewanella sp.

B4EWR5

Proteus mlrabilis

ClCGll

Streptococcus pneumoniae

C3KA14

Pseudomonas fluorescens

C3NU24

Vibrio cholerae serotype 01

A6TRD8

Alkaliphilus metalliredigens

QlCQT2

Streptococcus pyogenes serotype M12

Q9A029

Streptococcus pyogenes serotype Ml

C1D7P7

Laribacter hongkongensis

A9MSD6

Salmonella paratyphi B

B4TDT1

Salmonella heidelberg

87LNE9

Escherichia fergusonii

D3DGM9

Hydrogenobacter thermophilus

Q32GM7

Shigella dysenteriae serotype 1

Q8Z7Y3

Salmonella typhi

C6AM23

Aggregatibacter aphrophilus

Q926A1

listeria innocua serovar 6a

CG19468

Drosophila melanogaster

A2RDQ6

Streptococcus pyogenes serotype MS

D3V014

Xenorhabdus bovienii

F5ZL14

Streptococcus parauberis

E8T306

Thermovibrio ammonificans

F8LPH2

Streptococcus sa livarius

H8L634

Frateuria aurantia

F3YBJO

Melissococcus plutonius

AFA45162.1

Avibacterium paragallinarum

ZP_03338172.1

Salmonella enterica subsp.

enterica serovar Typhi str. 404ty

ZP_01797282.1

Haemophilus influenzae R3021

ZP_00683037.1

Xylella fastidiosa Ann-1

CBl82624.1

Bartonella schoenbuchensfs R1

CAA23976.1
Enterobacteria phage lambda

ZP_08900462.1

Gluconacetobacter oboediens 174Bp2

ZP_07805046.1

Helicobacter cinaedi CCUG 18818

CBl78688.1

Bartonella sp. AR 15-3

EEW20557.1

Listeria monocytogenes FSL R2-503

ZP_07873574.1

listeria ivanovii FSL F6-596

ZP_09071954.1

Paenibacillus larvae subsp. larvae B-3650

EGC95709.1

Escherichia fergusonii ECD227

yp_001648906.1
Enterobacteria phage Min27

EHC90429.1

Salmonella enterica subsp. enterica

serovar Rubislaw str. A4-653

YP_004666545.1

Myxococcus fulvus HW-1

ZP_03220723.1

Salmonella enter/ca subsp. enterica

serovar Javiana str.

GA_MM04042433

EHC91146.1

Salmonella enterica subsp. enterica

serovar Senftenberg str. A4543

AAF28115.1, AF153317_10

Shigella dysenteriae

ZP_06538095.1

Salmonella enterica subsp. enterica

serovar Typhi str. AG3

YP_630241.1

Myxococcus xanthus DK 1622

ZP_07683610.1

Shigella dysenteriae 1617

YP_003991543.1

Caldicellulosiruptor hydrothermalis 108

YP_004027154.1

Caldicellulosiruptor kristjanssonH 177R1B

YP_005368024.1

Corallococcus coralloides DSM 2259

YP_003952450.1

Stigmatella aurantiaca DW4/3-1

YP_001180241.1

Caldicellulosiruptor saccharolyticus DSM 8903

AAM08027.1

Providencia rettgeri

ABD63859.1

Lactococcus phage phismq86

yp_004023409.1

Caldicellulosiruptor kronotskyensis 2002

YP_005892581 .1

Neisseria meningitidis WUE 2594

CBX21356.1

Neisseria lactamica Y92-1009

ZP_02494581 .1

Burkholderia pseudomallei NCTC 13177

EDS78268.1

Clostridium botulinum C str. Eklund

yp_003845006.1

Clostridium cellulovorans 7438

yp_003662329.1

Xenorhabdus nematophila ATCC 19061

yp_002795496.1

laribacter hongkongensis HLHK9

yp_001063371.1

Burkholderia pseudomallei 668

YP_001320415.1

Alkaliphilus metalliredigens QYMF

AEU09829.1

Klebsiella pneumoniae

ABY28344.1

Vibrio cholerae 0139

YP_001101797.1

Yersinia ruckeri

EGT79392.1

Haemophilus haemolyticus M21639

yp_003084246.1
Cyanophage PSS2

ZP_06124041.2

Providencia rettgeri DSM 1131

NP_996684.1

lactococcus phage phiLC3

YP_005377560.1

Frateuria aurantia DSM 6220

ZP_05850920.1

Haemophilus influenzae NT127

CBW39270.1

Streptococcus phage 2167

ABW02831.1

Aggregatibacter aphrophilus NJ8700

EHE64346.1

Streptococcus pneumoniae EU-NPOl

yp_004456677.1

Melissococcus plutonius ATCC 35311

EGV02688.1

Streptococcus infantis SK970

EHL46421.1

Salmonella enterica subsp. enterica

serovar Montevideo str. SARB30

yp_004875641.1

Baciffus subtilis subsp. spizizenii TU-B-10

yp_003611805.1

Enterobacter cloacae subsp. cloacae ATCC 13047

yp_005352506.1

Photobacterium damselae subsp. damselae

ZP_08247669.1

Neisseria bacilliformis ATCC BAA-1200

EG086949.1

Clostridium botulinum C str. Stockholm

yp_004594671.1

Enterobacter aerogenes KCTC 2190

ZP_07797103.1

Pseudomonas aeruginosa 39016

CBl78049.1

Bartonella rochalimae ATCC BAA-1498

ZP_07922626.1

Fusobacterium sp. 3_1_5R

ZP_07042769.1

Comamonas testosteroni 544

2P_07367149.1

Pediococcus acidilactici DSM 20284

EHY70960.1

Salmonella enterica subsp. houtenae str. ATCC BAA-1581

yp-455377.1

Sodalis glossinidius str. ‘morsitans 1

y p _004479394.1

Streptococcus parauberis KCTC 11537

yp_001610320.1

Bartonella tribocorum CIP 105476

ZP_06552348.1

Klebsiella sp. 1_1_55

ZP_08564335.1

lactobacillus ruminis SPM0211

ZP_10073638.1

Haemophilus paraphrohaemolyticus HK411

EHN14107.1

Clostridium sporogenes PA 3679

ZP_04979099.1

Mannheimia haemolytica PHL213

ZP_06016835.1

Klebsiella pneumoniae subsp. rhinoscferomatis ATCC 13884

CBW39216.1

Streptococcus phage 8140

yp_005719596.1

Sinorhizobium me/iloti SM11

CBW38953.1

Streptococcus phage V22

ZP_04822781.1

Clostridium botulinum El str, 1BoNT E Beluga’

yp_596543.1

Streptococcus phage 9429.2

YP_003804225.1

Spirochaeta smaragdinae DSM 11293

yp_004549676.1

Sinorhizobium meliloti AK83

VP_001128217.1

Streptococcus pyogenes str. Manfredo

yp_360509.1

Carboxydothermus hydrogenoformans Z-2901

EHE76000.1

Streptococcus pneumoniae GA11426

YP_005953460.1

Klebsiella pneumoniae KCTC 2242

CCB95242.1

Streptococcus salivarius JIM8777

ZP_06691943.1

Acinetobacter sp. SH024

ZP_10036178.1

Burkholderia sp. Chl-1

ZP_03625981 .1

Streptococcus suis 89/1591

BAB53634.1

Mesorhizobium loti MAFF303099

YP_006078894.1

Streptococcus suis 5512

ZP_07075805.1

listeria monocytogenes FSL N l-017

yp_002128512.1

lodobacteriophage phiPLPE

YP_002971742.1

Bartonella grahamii as4aup

ZP_09588597.1

Fusobacterium sp. 12_1B

YP_003150705.1

Cryptobacterium curtum DSM 15641

ZP_01960732.1

Bacteroides caccae ATCC 43185

ZP_06011746.1

Leptotrichia goodfellowii F0264

NP_471091.1

listeria innocua Cli p11262

AAX89425.1

Vibrio cholerae non-01/non-0139

ZP_04661743.1

Acinetobacter baumannii AB900

YP_003993926.1

Halanaerobium hydrogeniformans

YP_001852190.1

Mycobacterium marinum M

ZP_07400314.1

Peptoniphilus duerdenii ATCC BAA-1640

ZP_08039150.1

Serratia symbiotica str. Tucson

YP_004208804.1

Bifidobacterium longum subsp. infantis 157F

YP_001397974.1

Campylobacter jejuni subsp. doylei 269.97

YP_006001389.1

Xylella fastidiosa subsp. fastidiosa GB514

AAF84457.1, AE003991_9

Xylella fastidiosa 9a5c

ZP_09143689.1

Acinetobacter sp. P8-3-8

ABD48929.1

Streptococcus phage M102

EIA80005.1

Campylobacter coli 1957

YP_004759254.1

Corynebacterium variabile DSM 44702

ZP_03297235.1

Collinsella stercoris DSM 13279

YP_001409109.1

Campylobacter curvus 525.92

EIB51049.1

Campylobacter jejuni subsp. jejuni 2008-988

EIA84440.1

Campylobacter coli 67-8

EIB08252.1

Campylobacter coli LMG 9860

EIA50333.1

Campylobacter coli 2685

EIA74674.1

Campylobacter coli 132-6

YP_003308034.1

Sebaldella termitidis ATCC 33386

YP_003325187.1

Xylanimonas cellulosilytica DSM 15894

EIA59740.1

Campylobacter coli 80352

ZP_01771318.1

Collinsella aerofaciens ATCC 25986

ZP_07889816.1

Aggregatibacter segnls ATCC 33393

YP_002475717.1

Haemophilus parasuis SH0165

YP_901551.1

Pelobacter propionicus DSM 2379

ZP_02479394.1

Haemophilus parasuis 29755

ZP_00372172.1

Campylobacter upsaliensis RM3195

YP_004748640.1

Acidithiobacillus caldus SM-1

ZP_05294088.1

Acidithiobacillus caldus ATCC 51756

ZP_07463816.1

Streptococcus gallolyticus subsp. gallolyticus TX20005

YP_070310.1

Yersinia pseudotuberculosis IP 32953

ZP_04898500.1

Burkholderia pseudomallei Pasteur 52237

EHK78038.1

Sinorhizobium meliloti CCNWSX0020

ZP_05880433.1

Vibrio furnissii CIP 102972

ZP_09397479.1
Acetobacteraceae bacterium AT-5844

ZP_04809499.1

Helicobacter pullorum MIT 98-5489

YP_002872416.1

Pseudomonas fluorescens SBW25

ZP_06157475.1

Photobacterium damselae subsp. damselae CIP 102761

YP_001327594.1

Sinorhizobium medlcae WSM419

YP_005511208.1

Hydrogenobacter thermophilus TK-6

YP_004662889.1

Simkania negevensis Z

YP_002281965.1

Rhizobium leguminosarum bv. trifolii WSM2304

YP_002731548.1

Persephonella marina EX-Hl

ZP_08768668.1

Thiocapsa marina 5811

ZP_08824405.1

Thiorhodococcus drewsii AZl

ZP_08977565.1

Desulfitobacterium metalllreducens DSM 15288

YP_003456916.1

Allochromatium vinosum DSM 180

YP_002417106.1

Vibrio splendidus LGP32

ZP_03497413.1

Thermus aquaticus Y51MC23

YP_001175737.1

Enterobacter sp. 638

ZP_08926729.1

Thiocystis violascens DSM 198

ZP_01947910.1

Vibrio cholerae 1587

ZP_08267258.1

Brevundimonas diminuta ATCC 11568

YP_004488616.1

Delftia sp. Csl-4

YP_005162586.1

Corynebacterium diphtheriae C7 (beta)

ADZ13631.1

Cronobacter phage ENT47670

ZP_09809048.1

Thiorhodovibrio sp. 970

YP_003010343.1

Paenibacillus sp. JDR-2

NP_930169.1

Photorhabdus luminescens subsp. laumondii TTOl

yp_001280242.1

Psychrobacter sp. PRwf-1

EHl54313.1

Aeromonas salmonicida subsp. salmonicida 01-8526

YP_192864.1

Gluconobacter oxydans 621H

ZP_08680759.1

Sporosarcina newyorkensis 2681

ZP_04762755.1

Acldovorax delafieldil 2AN

ZP_09077613.1

Paenibacil/us elgii 869

ZP_09899678.1

Methylomicrobium album BG8

ZP_07949994.1
Enterobacteriaceae bacterium 9_2_54FAA

YP_917501.1

Paracoccus denitrificans PD1222

ZP_09013441.1

Commensalibacter intestini A911

ZP_07908870.1

Mobiluncus curtisil ATCC 51333

ZP_00235039.1

listeria monocytogenes str. 1/2a F6854

EGH43274.1

Pseudomonas syringae pv. pisi str. 1704B

ZP_05297269.1

Listeria monocytogenes FSL J2-003

EGH87883.1

Pseudomonas syringae pv, lachrymans str. M301315

YP_001418489.1

Xanthobacter autotrophicus Py2

ZP_02971366.1

Ureaplasma parvurn serovar 6 str. ATCC 27818

YP_005087203.1

Rhodococcus phage REQ3

ZP_03771988.1

Ureaplasma urealyticum serovar 8 str. ATCC 27618

EDX53145.1

Ureaplasma urealyticum serovar 12 str. ATCC 33696

YP_003897727.1

Halomonas elongata DSM 2581

EHT14033.1

Klebsiella oxytoca 10-5250

ZP_02327781 .1

Paenibacillus larvae subsp. larvae BRL-230010

ZP_09377516.1

Hafnia alvei ATCC 51873

YP_002233655.1

Burkholderia cenocepacia J2315

YP_003374177.1

Gardnerella vaginalis 409-05

ZP_03805638.1

Proteus penneri ATCC 35198

ZP_05830211 .1

Acinetobacter baumannii ATCC 19606

ZP_06324416.1

Staphylococcus aureus subsp. aureus 0139

ZP_09243850.1

Pseudoalteromonas sp. BSi20495

ZP_03477183.1

Parabacteroides johnsonii DSM 18315

YP_001453480.1

Citrobacter koseri ATCC BAA-895

AAG00321.1

Borrelia hermsii

EHC73330.1

Salmonella enterica subsp. enterica serovar

Mississippi str. A4-633

VP_005098013.1

Salmonella phage SPNlS

EDZ19028.1

Salmonella enterica subsp, enterica

serovar Kentucky str. CDC 191

EFW50178.1

Shigella dvsenteriae CDC 74• 1112

ZP_03935819.1

Corynebacterium striatum ATCC 6940

ZP_08306099.1

Klebsiella sp. MS 92-3

ZP_07380439.1

Pantoea sp. aB

ZP_03840270.1

Proteus mirabilis ATCC 29906

ZP_03318967.1

Providencia alcalifaciens DSM 30120

AEW24552.1

Escherichia phage TL-2011b

ZP_03706978.1

Clostridium methylpentosum DSM 5476

VP_004327343.1

Erwinia phage phiEt88

ZP_08261052.1

Gemella sanguinis M325

ZP_06640797 .1

Serratia odorifera DSM 4582

VP_004355228.1

Pseudomonas brassicacearum subsp.

brassicacearum NFM421

ZP_03544152.1

Comamonas testosteroni KF-1

ZP_06714885.1

Edwardsiella tarda ATCC 23685

ZP_09015869.1

Brenneria sp. EniD312

CAC33455.1

Legionella pneumophila

EFU90864.1

Enterococcus faecalis TX0630

yp_002933453.1

Edwardsiella ictaluri 93-146

VP_003295659.1

Edwardsiella tarda EIB202

ZP_05361195.1

Acinetobacter radioresistens SK82

yp_673748.1

Chelativorans sp. BNCl

EGE24382.1

Moraxella catarrhalis 101P30Bl

VP_001886967.1

Clostridium botulinum B str. Eklund 17B

yp_003467094.1

Xenorhabdus bovieniiSS-2004

yp_003742593.1

Erwinia billingiae Eb661

AEl70845.1
EBPR podovirus 1

VP_002502020.1

Methylobacterium nodulans ORS 2060

VP_003931279.1

Pantoea vagans C9-1

ZP_09514368.1

Oceanicola sp. 5124

EHC38944.1

Salmonella enterica subsp, enterica

serovar Gaminara str. A4-567

VP_001335044.1

Klebsiella pneumoniae subsp.

pneumoniae MGH 78578

VP_004150652.1

Thermovibrio ammonificans HB-1

YP_001139941.1

Corynebacterium glutamicum R

NP_461568.2
Phage Gifsy-1

AEW24678.1

Escherichia phage TL-201lc

AAC26069.1

Salmonella enterica subsp.

enterica serovar Typhimurium

ZP_01237386.1

Vibrio angustum 514

VP_004630586.1

Corynebacterium ulcerans BR-AD22

ZP_05289346.1

Listeria monocytogenes FSL F2-515

EIC64366.1

Mycobacterium abscessus M93

VP_264268.1

Psychrobacter arcticus 273-4

ZP_07375909.1

Ahrensia sp. R2A130

VP_003477226.1

Thermoanaerobacter italicus Ab9

VP_004011824.1

Rhodomicrobium vannielii ATCC 17100

ZP_06074083.1

Acinetobacter radioresistens SH164

VP_132232.1

Photobacterium profundum SS9

VP_003657357.1

Segniliparus rotundus DSM 44985

ZP_09732369.1

Megamonas funiformis Y/T 11815

VP_235897.1

Pseudomonas syringae pv. syrlngae B728a

EGH59138.1

Pseudomonas syringae pv. maculicola str. ES4326

EGE19718.1

Moraxella catarrhalis BCl

ZP_07903104.1

Eubacterium saburreum DSM 3986

EFC94742.1

Clostridium hathewayi DSM 13479

ZP_08334226.1
Lachnospiraceae bacterium 9_1_43BFAA

ZP_09322413.1
Eubacteriaceae bacterium ACC19a

VP_003609870.1

aurkholderia sp. CCGE1002

ZP_060741S5.1

Bacteroides sp. 2_1_33B

YP_004168204.1

Nitratifractor salsuginis DSM 16511

YP_003162220.1

Jonesia denitrificans DSM 20603

AAA22084.1

Agrobacterium tumefaciens

AAS46728.1

Aeromonas hydrophila

CBL42165.1
butyrate-producing bacterium SS3/4

ZP_08499194.1

Enterobacter hormaechei ATCC 49162

ACD38903.1

Pseudomonas aeruginosa

yp_005098041.1

Pseudomonas phage phi297

yp_003438695.1

Klebsiella variicola At-22

EGD06615.1

Burkholderia sp. TJl49

EHS95796.1

Klebsiella oxytoca 10-5243

yp_001863170.1

Burkholderia phymatum STM815

yp_001667964.1

Pseudomonas putida GB-1

yp_002238960.1

Klebsiella pneumoniae 342

yp_006011723.1

Shewanella putrefaciens 200

yp_005321079.1

Saprospfra grandis str. Lewin

EIC70403.1

Mycobacterium abscessus M94

ZP_09433274.1

Bradyrhizobium sp. STM 3843

8A185059.1

Bacillus subtilis subsp. natto BEST195

yp_516203.1

Sodalis phage phiSGl

ZP_06299504.1

Parachlamydia acanthamoebae str. Hall's coccus

EGP42731.1

Achromobacter xylosoxidans AXX-A

ZP_03311406.1

Desulfovibrio piger ATCC 29098

ZP_04945782.1

Burkholderia dolosa AU0158

ZP_07200498.1
delta proteobacterium NaphS2

ZP_07741429.1

Vibrio caribbenthicus ATCC BAA-2122

yp_003084663.1

Dyadobacterfermentans DSM 18053

ZP_02163271.1

Kordia algicida OT-1

yp_005357245.1

Flavobacterium indicum G PTSAl00-9

ZP_06177392.1

Vibrio harveyi 1DA3

YP_004655025.1

Runella slithyformis DSM 19594

yp_001196868.1

Flavobacterium johnsoniae UW101

yp_004988339.1

Owenweeksia hongkongensis DSM 17368

yp_006017652.1

Riemerella anatipestifer RA-GD

ZP_02183546.1

Flavobacteriales bacterium ALC-1

yp_003998593.1

Leadbetterella byssophila DSM 17132

YP_004843584.1

Flavobacterium branchiophilum FL-15

YP_006190082.1

Paenibacillus mucilaginosus K02

ZP_09236879.1

Pseudoafteromonas sp. BSi20439

yp_002911448.1

Burkholderia glumae BGRl

yp_004238525.1

Weeksella virosa DSM 16922

ZP_09524021.1

Myroides odoratimimus CCUG 10230

YP_004776003.1

Cyclobacterium marinum DSM 745

YP_004581188.1

Lacinutrix sp. SH-3-7-4

ZP_02435739.1

Bacteroides stercoris ATCC 43183

yp_005208095.1

Pseudomonas fluorescens F113

YP_004445529.1

Haliscomenobacter hydrossis DSM 1100

yp_005930618.1

Pseudomonas putida BIRD-1

YP_005006679.1

Niastella koreensis GR20-10

yp_005633953.1

Vibrio cholerae LMA3984-4

yp_004789848.1

Muricauda ruestringensis DSM 13258

yp_005869785.1

Lactococcus garvieae Lg2

YP_003907168.1

Burkholderia sp. CCGE1003

YP_006015496.1

Staphylococcus pseudintermedius ED99

YP_004941085.1

Flavobacterium columnare ATCC 49512

YP_003700049.1

Bacillus selenitireducens MLS10

YP_001760744.1

Shewanella woodyi ATCC 51908

yp_005865109.1

lactobacillus rhamnosus GG

yp_004272912.1

Pedobacter saltans DSM 12145

yp_001295947.1

Flavobacterium psychrophilum JIP02/86

NP_212876.2

Borrelia burgdorferi B31

yp_005034813.1

Bacteriovorax marinus SJ

yp_369306.1

Burkholderia sp. 383

YP_005820203.1

Fibrobacter succinogenes subsp. succinogenes 585

yp_002005623.1

Cupriavidus taiwanensis LMG 19424

yp_348340.1

Pseudomonas fluorescens Pf0-1

yp_004380540.1

Pseudomonas mendocina NK-01

yp_005858762.1

Lactobacillus casei BD-11

YP_692011.1

Alcanivorax borkumensis SK2

yp_001099394.1

Herminiimonas arsenicoxydans

YP_004166442.1

Cellulophaga algicola DSM 14237

ZP_09641960.1

Odoribacter laneus YIT 12061

EHE92415.1

Lactococcus lactis subsp. cremoris CNCM 1-1631

YP_583727.1

Cupriavidus metallidurans CH34

ZP_02206967.1

Coprococcus eutactus ATCC 27759

y p _004890503.1

Lactobacillus plantarum WCFSl

ZP_08586327.1

Bacteroides sp. 1_1_30

YP_005672087.1

Clostridium acetobutylicum EA 2018

ZP_09521413.1
Lachnospiraceae bacterium ACC2

EHE89971.1

Streptococcus thermophilus CNCM 1-1630

ZP_02996105.1

Clostridium sporogenes ATCC 15579

yp_396489.1

Lactobacillus sakei subsp. sakei 23K

YP_004739144.1

Zobelfia galactanivorans

y p_004993437.1

Vibrio furnissii NCTC 11218

y p_005092407.1

Oceanimonas sp, GK1

yp_253435.1

Staphylococcus haemolyticus JCSC1435

yp_005848993.1

Lactobacillus fermentum CECT 5716

yp_006180809.1

Halobacillus halophilus DSM 2266

yp_574526.1

Chromohalobacter salexigens DSM 3043

YP_004396695.1

Clostridium botulinum BKT015925

NP_889567.1

Bordetella bronchiseptica RB50

YP_006217717.1

Providencia stuartii MRSN 2154

yp_001155602.1

Polynucleobacter necessarius subsp.

asymbioticus QLW-PlDMWA-1

ZP_09337816.1

Tannerella sp. 6_1_58FAA_CT1

ZP_09023107.1

Alistipes indistinctus YIT 12060

yp_661287.1

Pseudoalteromonas atlantica T6c

NP_722442.1

Streptococcus mutans UA159

YP_002265207.1

Aliivibrio salmonicida LF11238

ZP_09188055.1

Halomonas boliviensis LC1

yp_003811665.1
gamma proteobacterium HdNl

ZP_03302243.1

Bacteroides dorei DSM 17855

EHG11465.1

Streptococcus intermedius F0395

YP_004726213.1

Weissella koreensis KACC 15510

ZP_08890725.1

Lactobacillus sp. 7_1_47FAA

yp_004977003.1

Burkholderia sp. Yl23

yp_927263.1

Shewanella amazonensis SB2B

ZP_08339829.1
Lachnospiraceae bacterium 2_1_46FAA

yp_274842.1

Pseudomonas syringae pv. phaseolicola 1448A

YP_005975666.1

Pseudomonas aeruginosa M18

YP_006035100.1

Streptococcus gallolyticus subsp. gallolyticus ATCC 43143

YP_005412494.1

Streptococcus pyogenes MGAS1882

EH078754.1

Fusobacterium nucleatum subsp. animalis OT 420

YP_004317691.1

Sphingobacterium sp. 21

YP_003163767.1

Leptotrichia buccalis C-1013-b

N P_693974.1

Oceanobacillus iheyensis HTE831

ZP_09148363.1

Clostridium hathewayi WAL-18680

ZP_02073778.1

Clostridium sp. L2-50

VP_005862099.1

Lactobacillus johnsonii DPC 6026

yp_006085650.1

Streptococcus suis A7

YP_786508.1

Bordetella avium 197N

EHG 25612.1

Selenomonas noxia F0398

YP_942451.1

Psychromonas ingrahamii 37

ZP_02421015.1

Anaerostipes caccae DSM 14662

yp_005147109.1

Clostridium sp. BN LllOO

ZP_03800678.1

Coprococcus comes ATCC 27758

YP_001631170.1

Bordetella petrii DSM 12804

yp_005739407.1

Staphylococcus aureus subsp. aureus JKD6159

YP_005863385.1

Lactobacillus salivarius CECT 5713

yp_856547.1

Aeromonas hydrophila subsp. hydrophila ATCC 7966

YP_005014978.1

Tannerella forsythia ATCC 43037

YP_004543651.1

Desulfotomaculum ruminis DSM 2154

ZP_08850119.1

Dorea formicigenerans 4_6_53AFAA

yp_005938868.1

Pseudomonas stutzeri DSM 4166

yp_004971910.1

Desu/fosporosinus orientis DSM 765

YP_005704426.1

Enterococcus faecalis 62

yp_002930165.1

Eu bacterium eligens ATCC 27750

ZP_09112782.1

Enterococcus saccharolyticus 30_1

yp_004846059.1

Pseudogu lbenkiania sp. NH8B

yp_001438598.1

Cronobacter sakaza kii ATCC BAA-894

N P_718112.1

Shewanella oneidensis MR-1

yp_005433748.1

Selenomonas ruminantium subsp. lactilytica TAM6421

ZP_02438733.1

Clostridium sp. 552/1

ZP_08616825.1
lachnospiraceae bacterium 1_4_56FAA

ZP_09154646.1

Johnsonella ignava ATCC 51276

yp_001141717.1

Aeromonas salmonicida subsp. salmonicida A449

yp_006029734.1

Ralstonia solanacearum Po82

ZP_08470204.1

Oysgonomonas mossii DSM 22836

ZP_02033987.1

Para bacteroides merdae ATCC 43184

ZP_08474773.1

Dysgonomonas gadei ATCC BAA-286

ZP_03010636.1

Bacteroides coprocola DSM 17136

yp_001983252.1

Cellvibrio japonicus Ueda107

ZP_08603145.1
lachnospiraceae bacterium 5_1_57FAA

YP_005474943.1

Spirocha eta africa na DSM 8902

ZP_09736507.1

Facklam ia languida CCUG 37842

ZP_05127187.1
gamma proteobacterium NORS-3

ZP_04449563.1

Catonella morbi ATCC 51271

ZP_02024907.1

Eubacterium ventriosum ATCC 27560

ZP_03712468.1

Eikenel la corrodens ATCC 23834

YP_004512397.1

Methylomonas methanica MC09

ZP_09334735.1

Citrobacter freundii 4_7_47CFAA

ZP_03781365.1

Blautia hvdrogenotrophica DSM 10507

VP_004426052.1

Alteromonas macleodii str. ‘Deep ecotype’

ZP_03762445.1

Clostridium asparagiforme DSM 15981

VP_002800018.1

Azotobacter vinelandii DJ

ZP_09532735.1
Lachnospiraceae bacterium 7_1_58FAA

ZP_04854710.1

Paenibacillus sp. oral taxon 786 str. 014

VP_004468160.1

Alteromonas sp. SN2

VP_520049.1

Desulfitobacterium hafniense YSl

VP_385574.1

Geobacter metallireducens GS-15

ZP_08336670.1
lachnospiraceae bacterium 3_1_46FAA

VP_004915903.1

Methvlom icrobium a lca liphilum

VP_527033.1

Saccharophagus degradans 2-40

YP_005318817.1

Me/issococcus plutonius DAT561

VP_004874525.1

Tavlorella asi nigenita lis MCE3

YP_005851623.1

lactobacillus delbrueckii subsp. bulga ricus 2038

ZP_09326776.1

Oribacterium sp. ACB1

ZP_03461890.1

Bacteroides pectinophilus ATCC 43243

VP_001571105.1

Salmonella enterica subsp. arizonae

serovar 62:z4, z23:-- str. RSK2980

ZP_03166627.1

Ruminococcus lactaris ATCC 29176

ZP_03755701.1

Roseburia inulinivorans DSM 16841

ZP_03719260.1

Neisseria flavescens N RL30031/H 210

VP_005073546.1

Paenibacillus terrae H PL-003

VP_002312664.1

Shewanella piezotolerans WP3

VP_005005652.1

Pediococcus cfaussenii ATCC BAA-344

VP_003126133.1

Chitinophaga pinensis DSM 2588

ZP_08258595.1

Gemella haemolvsans M341

VP_001473852.1

Shewanella sediminis HAW-EB3

yp_001093802.1

Shewanella loihica PV-4

ZP_03777231.1

Clostridium hvlemonae DSM 15053

ZP_08149693.1
Lachnospiraceae bacterium 4_1_37FAA

VP_001681197.1

Heliobacterium modesticaldum leel

N P_951231.1

Geobacter sulfurreducens PCA

ZP_02035690.1

Bacteroides ca pillosus ATCC 29799

VP_003556874.1

Shewanel la violacea DSS12

VP_037013.1

Bacil l us thuringiensis serovar konkukian str. 9727

VP_005272800.1

Shewanella baltica 05678

ZP_09537413.1
Erysipelotrichaceae bacterium 21_3

VP_00441 1234.1

Spirochaeta coccoides DSM 17374

VP_003428708.1

Bacillus pseudofirmus OF4

yp_006020740.1

Shewanella ba ltica BA175

ZP_09120679.1

Prevotella sp. oral taxon 302 str. F0323

ZP_02084161.1

Clostridium bolteae ATCC BAA-613

ZP_01725297.1

Bacillus sp. 814905

ZP_08604142.1
Lachnospiraceae bacterium 3_1_57FAA_CT1

yp_006231372.1

Bacillus sp. JS

ZP_04451575.1

Abiotrophia defectiva ATCC 49176

ZP_04855253.1

Ruminococcus sp. 5_1_39B_FAA

ZP_09697329.1

Coprobacil l us sp. 8_2_54BFAA

VP_001182934.1

Shewanella putrefaciens CN-32

VP_004048639.1

Neisseria lactamica 020-06

ZP_03682223.1

Catenibacterium mitsuokai DSM 15897

y p _005889683.1

Neisseria gonorrhoeae TCDC-NGD8107

ZP_08331597.1
Lachnospiraceae bacterium 6_1_63FAA

y p_091248.1

Bacillus licheniformis DSM 13 • ATCC 14580

ZP_09049102.1

Clostridium sp. 7_3_54FAA

VP_004871614.1

Glaciecola nitratireducens FR1064

VP_003464587.1

Listeria seeligeri serovar 1/2b str. SLCC3954

VP_512292.1

Escherichia phage phiVlO

ZP_09545227.1

Eubacterium sp. 3_1_31

ZP_02094048.1

Parvimonas micra ATCC 33270

VP_004560942.1

Erysipelothrix rhusiopathiae str. Fujisawa

VP_005224087.1

Treponema pallidum subsp. pallidum DAL-1

ZP_03290838.1

Clostridium nexile DSM 1787

ZP_02867996.1

Clostridium spiroforme DSM 1552

VP_051100.1

Pectobacterium atrosepticum SCRl1043

VP_004247313.1

Sphaerochaeta globus str. Buddy

VP_005199476.1

Rahnella aquatilis CIP 78.65 = ATCC 33071

VP_002472037.1

Clostridium kluyveri NBRC 12016

EHP49112.1

Clostridium perfringens WAL-14572

ZP_04454959.1

Shuttleworthia satelles DSM 14600

VP_005061390.1

Sphaerochaeta pleomorpha str. Grapes

VP_005850725.1

Lactobacillus helveticus H lO

ZP_09590809.1

Prevotella micans F0438

VP_005993583.1

Pantoea ananatis PA13

VP_003519587.1

Pantoea ananatis LMG 20103

VP_005174459.1

Leuconostoc mesenteroides subsp. mesentero1des 118

VP_001377632.1

Anaeromyxobacter sp. Fw109-5

yp_006024370.1

Serratia sp. AS13

ZP_09554100.1

Prevotella maculosa OT 289

VP_005269748.1

Acetobacterium woodii DSM 1030

VP_004838017.1

Roseburia hominis A2-183

BAH69619.1

Mycoplasma fermentans PG18

VP_313909.1

Thiobacillus denitrificans ATCC 25259

VP_006005235.1

Yersinia enterocoJitica subsp. palearctica Yll

VP_002603986.1

Desulfobacterium autotrophicum HRM2

yp_001616277.1

Sorangium cellulosum 1 $0 ce 561

ZP_09342658.1

Subdoligranulum sp. 4_3_54A2FAA

ZP_09103791.1

Prevotella histicola F0411

VP_005538542.1

Arcobacter butzleri ED-1

YP_003960581.1

Eubacterium limosum KlST612

VP_003655761 .1

Arcobacter nitrofigilis DSM 7299

VP_004704993.1

Leuconostoc sp. C2

VP_005888312.1

Mycoplasma hyopneumoniae 168

ZP_03717663.1

Eubacterium hallii DSM 3353

VP_004263391.1

Cellulophaga lytica DSM 7489

VP_003856575.1

Mycoplasma hyorhinis HUB-1

VP_005905159.1

Mycoplasma hyorhinis MCLD

ZP_09738126.1

Helcococcus kunzii ATCC 51366

ZP_05364429.1

Campylobacter showae RM3277

VP_005059662.1

Granulicella mallensis MP5ACTX8

VP_005785121.1

Helicobacter pylori 83

VP_005553834.1

Arcobacter sp, L

VP_004868821.1

Gluconacetobacter xylinus NBRC 3288

ZP_02442082.1

Anaerotruncus colihominis DSM 17241

VP_004438805.1

Treponema brennaborense DSM 12168

EH012592.1

Myroides odoratimimus CIP 101113

VP_744021.1

Granulibacter bethesdensis CGDN/Hl

ZP_04550622.1

Bacteroides sp. 2_2_4

AAF82113.1

Campylobacter je juni

ZP_06202195.1

Bacteroides sp. 020

yp_001125680.1

Geobacillus thermodenitrificans NGS0-2

ZP_04540182.1

Bacteroides sp. 9_1_42FAA

CA090389.1

Microcystis aeruginosa PCC 7806

ZP_07811148.1

Bacteroides fragilis 3_1_12

ZP_09999130.1

l mtechella ha lotolera ns Kl

yp_001516275.1

Acaryochloris marina MBlC11017

yp_005442188.1

Caldilinea aerophila DSM 14535 = N BRC 104270

yp_004270153.1

Planctomyces brasiliensis DSM 5305

ZP_08791189.1

Bacteroides sp. Dl

YP_722968.1

Trichodesmium erythraeum 1MS101

TABLE 6

Recombinases.

RECOMBINASES

ZP_04451575.1

Abiotrophia defectiva ATCC 49176

VP_001516275.1

Acaryochloris marina MBIC11017

ZP_09397479.1

Acetobacteraceae bacterium AT-5844

VP_005269748.1

Acetobacterium woodii DSM 1030

EGP42731.1

Achromobacter xylosoxidans AXX-A

ZP_05294088.1

Acidithiobacillus ca Idus ATCC 51756

VP_004748640.1

Acidithiobacillus caldus SM-1

ZP_04762755.1

Acidovorax delafieldii 2AN

ZP_04661743.1

Acinetobacter baumannii AB900

ZP_05830211.1

Acinetobacter baumannii ATCC 19606

ZP_06074083.1

Acinetobacter radioresistens SH164

ZP_06074084.1

Acinetobacter radioresistens SH164

ZP_05360445.1

Acinetobacter radioresistens SK82

ZP_05361614.1

Acinetobacter radioresistens SK82

ZP_05361195.1

Acinetobacter radioresistens SK82

ZP_09143689.1

Acinetobacter sp. P8-3-8

ZP_09142708.1

Acinetobacter sp. P8-3-8

ZP_06691943.1

Acinetobacter sp. SH024

VP_002995586.1

Aeromonas hydrophila

AAS46728.1

Aeromonas hydrophila

VP_856547.1

Aeromonas hydrophila subsp. hydrophila ATCC 7966

EHl54313.1

Aeromonas salmonicida subsp. salmonicida 01-B526

VP_001141717.1

Aeromonas salmonicida subsp. salmonicida A449

VP_003006972.1

Aggregatibacter aphrophilus NJ8700

ABW02831.1

Aggregatibacter aphrophilus NJ8700

ZP_07889816.1

Aggregatibacter segnis ATCC 33393

AAA22084.1

Agrobacteri um tumefaciens

ZP_07375909.1

Ahrensia sp. R2A130

VP_692011.1

Alcanivorax borkumensis SK2

VP_002265207.1

Aliivibrio salmonicida LFl1238

VP_002264570.1

Aliivibrio salmonicida LFl1238

ZP_09023107.1

Alistipes indistinctus VIT 12060

VP_001320415.1

Alkaliphilus metalliredigens QVMF

VP_001321158.1

Alkaliphilus metalliredigens QVMF

VP_003456916.1

Allochromati um vinosum DSM 180

VP_004426052.1

Alteromonas macleodiistr. ‘Deep ecotype’

VP_004427628.1

Alteromonas macleodiistr. ‘Deep ecotype’

VP_004468160.1

Alteromonas sp. SN2

VP_001377632.1

Anaeromyxobacter sp. Fw109-5

ZP_02421015.1

Anaerostipes caccae DSM 14662

ZP_02442082.1

Anaerotruncus colihominis DSM 17241

VP_005538542.1

Arcobacter butzleri ED-1

VP_003655761.1

Arcobacter nitrofigilis DSM 7299

VP_005553834.1

Arcobacter sp. L

AFA45162.1

Avibacterium paragallinarum

VP_002800018.1

Azotobacter vinelandii DJ

VP_091248.1

Bacillus licheniformis DSM 13 = ATCC 14580

VP_003428708.1

Bacillus pseudofirmus OF4

VP_003700049.1

Bacillus selenitireducens MLS10

ZP_01725297.1

Bacillus sp. B14905

VP_006231372.1

Bacillus sp. JS

BAl85059.1

Bacillus subtilis subsp. natto BEST195

YP_004875641.1

Bacillus subtilis subsp. spizizenii TU-B-10

yp_037013.1

Bacillus thuringiensis serovar konkukian str. 97-27

YP_005034813.1

Bacteriovorax marinus SJ

yp_005036300.1

Bacteriovorax marinus SJ

ZP_01960732.1

Bacteroides caccae ATCC 43185

ZP_02035690.1

Bacteroides capillosus ATCC 29799

ZP_03010636.1

Bacteroides coprocola DSM 17136

ZP_03302243.1

Bacteroides dorei DSM 17855

ZP_07811148.1

Bacteroides fragilis 3_1_12

ZP_03461890.1

Bacteroides pectinophilus ATCC 43243

ZP_08S86327.1

Bacteroides sp. 1_1_30

ZP_06074155.1

Bacteroides sp. 2_1_338

ZP_04550622.1

Bacteroides sp. 2_2_4

ZP_04540182.l

8acteroides sp. 9_1_42FAA

ZP_08791189.1

8acteroides sp. Dl

ZP_06202195.1

8acteroides sp. D20

ZP_02435739.l

8acteroides stercoris ATCC 43183

ZP_02434797.1

8acteroides stercoris ATCC 43183

YP_002971742.1

8artonella grahamii as4aup

CBl78049.1

Bartonella rochalimae ATCC BAA-1498

(8182624.1

8artonella schoenbuchensis Rl

C8182531.1

Bartonella schoenbuchensis Rl

CBl81728.1

8artonella schoenbuchensis Rl

CBl82748.1

8artonella schoenbuchensis Rl

(8182323.1

8artonella schoenbuchensis Rl

C8178688.1

Bartonella sp. AR 15-3

yp_001610320.1

Bartonella tribocorum CIP 105476

yp_001609377.1

8artonella tribocorum CIP 105476

yp_004208804.1

8ifidobacterium longum subsp. infantis 157F

ZP_03781365.1

Blautia hydrogenotrophica DSM 10507

yp_786508.1

Bordetella avium 197N

NP_889567.1

8ordetella bronchiseptica RB50

YP_001631170.1

Bordetella petrii DSM 12804

NP_212876.2

Borrelia burgdorferi B31

AAG00321.1

Borrelia hermsii

ZP_09433274.1

Bradyrhizobium sp. STM 3843

ZP_09015869.1

Brenneria sp. EniD312

ZP_08267258.1

Brevundimonas diminuta ATCC 11568

YP_002233655.1

8urkholderia cenocepacia J2315

ZP_04945782.1

Burkholderia dolosa AU0158

YP_002911448.1

Burkholderia glumae 8GR1

YP_001863170.1

Burkholderia phymatum STM815

VP_001063371.1

Burkholderia pseudomallei 668

ZP_02494581.1

Burkholderia pseudomallei NCTC 13177

ZP_04898500.1

Burkholderia pseudomallei Pasteur 52237

YP_369306.1

Burkholderia sp. 383

YP_003609870.1

8urkholderia sp. CCGE1002

YP_003907168.1

Burkholderia sp. CCGE1003

ZP_10036178.1

8urkholderia sp. Chl-1

EGD06615.1

8urkholderia sp. TJl49

VP_004977003.1

Burkholderia sp. Vl23

CBL42165.1
butyrate-producing bacterium SS3/4

VP_003991543.1

Caldicellulosiruptor hydrothermalis 108

VP_004027154.1

Caldicellulosiruptor kristjanssonii 177R1B

VP_004023409.1

Caldicellulosiruptor kronotskyensis 2002

VP_001180241.1

Caldicellulosiruptor saccharolyticus DSM 8903

VP_001181365.1

Caldicellulosiruptor saccharolyticus DSM 8903

VP_005442188.1

Caldilinea aerophila DSM 14535 = NBRC 104270

EIA74674.1

Campylobacter coli 132-6

EIA80005.1

Campylobacter coli 1957

EIA50333.1

Campylobacter coli 2685

EIA84440.1

Campylobacter coli 67-8

EIA59740.1

Campylobacter coli 80352

EIB08252.1

Campylobacter coli LMG 9860

VP_001409109.1

Campylobacter curvus 525.92

AAF82113.l

Campylobacter jejuni

VP_001397974.1

Campylobacter jejuni subsp. doylei 269.97

EIB51049.1

Campylobacter jejuni subsp. jejuni 2008-988

ZP_05364429.1

Campylobacter showae RM3277

ZP_00372172.1

Campylobacter upsaliensis RM3195

VP_360509.1

Carboxydothermus hydrogenoformans Z-2901

ZP_03682223.1

Catenibacterium mitsuokai DSM 15897

ZP_04449563.1

Catonella morbi ATCC 51271

VP_004166442.1

Cellulophaga algicola DSM 14237

VP_004166181.1

Cellulophaga a/gico/a DSM 14237

VP_004263391.1

Cellulophaga lytica DSM 7489

VP_0019832S2.1

Cellvibrio japonicus Ueda107

VP_673748.1

Chelativorans sp. BNCl

VP_003126133.1

Chitinophaga pinensis DSM 2588

VP_574526.1

Chromohalobacter sa/exigens DSM 3043

ZP_09334735.1

Citrobacter freundii 4_7_47CFAA

VP_001453480.i

Citrobacter koseri ATCC BAA-895

VP_005672087.1

Clostridium acetobutylicum EA 2018

ZP_03762445.1

Clostridium asparagiforme DSM 15981

ZP_02084161.1

Clostridium bolteae ATCC BAA-613

VP_001886967.1

Clostridium botulinum B str. Eklund 178

VP_004396695.1

Clostridium botulinum BKT015925

EDS78268.1

Clostridium botulinum C str. Eklund

EDS76145.l

Clostridium botulinum C str. Eklund

EG086949.l

Clostridium botulinum C str. Stockholm

ZP_04822781.1

Clostridium botulinum El str. ‘BoNT E Beluga’

VP_003845006.1

Clostridium cellulovorans 7438

EFC94742.1

Clostridium hathewayi DSM 13479

EFD01272.1

Clostridium hathewayi DSM 13479

ZP_09148363.1

Clostridium hathewayi WAL-18680

ZP_03777231.1

Clostridium hylemonae DSM 15053

VP_002472037.1

Clostridium kluyveri NBRC 12016

ZP_03706978.1

Clostridium methylpentosum DSM 5476

ZP_03706281.1

Clostridium methylpentosum DSM 5476

ZP_03290838.1

Clostridium nexile DSM 1787

EHP49112.1
•clostridium perfringens WAL-14572

ZP_09049102.1

Clostridium sp. 7_3_54FAA

yp_005147109.1

Clostridium sp. BNLllOO

ZP_02073778.1

C/ostridium sp. L2-50

ZP_02438733.1

Clostridium sp. SS2/1

ZP_02867996.1

Clostridium spiroforme DSM 1552

ZP_02996105.1

C/ostridium sporogenes ATCC 15579

EHN14107.1

C/ostridium sporogenes PA 3679

ZP_01771318.1

Collinsel/a aerofaciens ATCC 25986

ZP_03297235.1

Collinsella stercoris DSM 13279

ZP_03544152.1

Comamonas testosteroni KF-1

ZP_07042769.1

Comamonas testosteroni S44

ZP_09013441.1

Commensa /ibacter intestlni A911

ZP_ 09697329.1

Coprobacillus sp. 8_2_54BFAA

ZP_03800678.1

Coprococcus comes ATCC 27758

ZP_02206967.1

Coprococcus eutactus ATCC 27759

yp_005368024.1

Corallococcus coralloides DSM 2259

yp_005162586.1

Corynebacterium diphtheriae C7 (beta)

yp_001139941.1

Corynebacterium glutamlcum R

ZP_03935819.1

Corynebacterium striatum ATCC 6940

yp_004630586.1

Corynebacterium ulcerans BR-AD22

yp_004759254.1

Corynebacterium variabile DSM 44702

ADZ13631.1

Cronobacter phage ENT47670

yp_001438598.1

Cronobacter sakazakii ATCC BAA-894

yp_003150705.1

Cryptobacterium curtum DSM 15641

yp_583727.1

Cupriavidus metallidurans CH34

yp_002005623.1

Cupriavidus taiwanensis LMG 19424

YP_003084246.1
Cyanophage PSS2

ACY75805.1
Cyanophage PSS2

yp_004776003.1

Cyclobacterium marinum DSM 745

yp_004488616.1

Delftia sp. Csl-4

ZP_07200498.1
delta proteobacterium NaphS2

yp_520049.1

Desulfitobacterium hafniense Y51

ZP_08977565.1

Desulfitobacterium metallireducens DSM 15288

yp_002603986.1

Desulfobacterium autotrophicum HRM2

yp_004971910.1

Desulfosporosinus orientis DSM 765

yp_004543651.1

Desulfotomaculum ruminis DSM 2154

ZP_03311406.1

Desulfovibrio piger ATCC 29098

ZP_08850119.1

Dorea formicigenerans 4_6_53AFAA

yp_003084663.1

Dyadobacter fermentans DSM 18053

yp_003088487.1

Dyadobacter fermentans DSM 18053

ZP_08474773.1

Dysgonomonas gadei ATCC BAA-286

ZP_08473950.1

Dysgonomonas gadei ATCC BAA-286

ZP_08470204.1

Dysgonomonas mossii DSM 22836

AE /70845.1
EBPR podovirus 1

yp_002933453.1

Edwardsie/la ictaluri 93-146

yp_002934200.1

Edwardsiel/a ictaluri 93-146

ZP_06714885.1

Edwardsiella tarda ATCC 23685

yp_003295659.1

Edwardsiella tarda EIB202

ZP_03712468.1

Eikenella corrodens ATCC 23834

YP_004594671.1

Enterobacter aerogenes KCTC 2190

yp_004594431.1

Enterobacter aerogenes KCTC 2190

YP_003611805.1

Enterobacter cloacae subsp. cloacae ATCC 13047

ZP_08499194.1

Enterobacter hormaechei ATCC 49162

YP_001175737.1

Enterobacter sp. 638

CAA23976.1

Enterobacteria phage lambda

yp_001648906.1

Enterobacteria phage Min27

ZP_07949994.1
Enterobacteriaceae bacterium 9_2_54FAA

YP_005704426.1

Enterococcus faecalis 62

EFU90864.1

Enterococcus faecalis TX0630

ZP_09112782.1

Enterococcus saccharolyticus 30_1

yp_003742593.1

Erwinia billingiae Eb661

yp_003740823.1

Erwinia billingiae Eb661

yp_004327343.1

Erwinia phage phiEt88

YP_004560942.1

Erysipelothrix rhusiopathiae str. Fujisawa

ZP_09537413.1
Erysipelotrichaceae bacterium 21_3

EGC95709.1

Escherichia fergusonii ECD227

YP_512292.1

Escherichia phage phiVlO

AEW24552.1

Escherichia phage TL-20l b

AEW24678.1

Escherichia phage TL-20llc

ZP_09322413.1
Eubacteriaceae bacterium ACC19a

yp_002930165.1

Eubacterium eligens ATCC 27750

ZP_03717663.1

Eubacterium hallii DSM 3353

YP_003960581.1

Eubacterium limosum KIST612

ZP_07903104.1

Eubacterium saburreum DSM 3986

ZP_09545227.1

Eubacterium sp. 3_1_31

ZP_02024907.1

Eubacterium ventriosum ATCC 27560

ZP_09736507.1

Facklamia languida CCUG 37842

yp_005820203.1

Fibrobacter succinogenes subsp. succinogenes S85

ZP_02183546.1
Flavobacteriales bacterium ALC-1

yp_004843584.1

Flavobacterium branchiophilum FL-15

yp_004843521.1

Flavobacterium branchiophilum FL-15

YP_004941085.1

Flavobacterium columnare ATCC 49512

yp_004941882.1

Flavobacterium columnare ATCC 49512

yp_005357245.1

Flavobacterium indicum GPTSAl00-9

YP_005357968.1

Flavobacterium indicum GPTSAl00-9

yp_001196868.1

Flavobacterium johnsoniae UW101

yp_001195256.1

Flavobacterium johnsoniae UW101

yp_001295947.1

Flavobacterium psychrophilum J/POZ/86

yp_001296638.1

Flavobacterium psychrophilum JIP02/86

yp_005377560.1

Frateuria aurantia DSM 6220

EH078754.1

Fusobacterium nucleatum subsp. animalis OT 420

ZP_09588597.1

Fusobacterium sp. 12_1B

ZP_09586364.1

Fusobacterium sp. 12_1B

ZP_07922626.1

Fusobacterium sp. 3_1_5R

YP_003811665.1
gamma proteobacterium HdNl

ZP_05127187.1
gamma proteobacterium NOR5-3

yp_003374177.1

Gardnerella vaginalis 409-05

ZP_08258595.1

Gemella haemolysans M341

ZP_08261052.1

Gemella sanguinis M325

yp_001125680.1

Geobacillus thermodenitrificans NG80-2

yp_385574.1

Geobacter metallireducens GS-15

NP_951231.1

Geobacter sulfurreducens PCA

YP_004871614.l

Glaciecola nitratireducens FR1064

ZP_08900462.l

Gluconacetobacter oboediens 174Bp2

ZP_08900481.l

Gluconacetobacter oboediens 174Bp2

ZP_08900554.l

Gluconacetobacter oboediens 174Bp2

yp_004868821.l

Gluconacetobacter xylinus NBRC 3288

yp_192864.1

Gluconobacter oxydans 621H

VP_191186.1

Gluconobacter oxydans 621H

VP_744021.1

Granulibacter bethesdensis CGDNIHl

YP_005059662.l

Granulicella mallensis MP5ACTX8

EGT79392.1

Haemophilus haemolyticus M21639

ZP_05850920.1

Haemophilus influenzae NT127

ZP_01797282.1

Haemophilus influenzae R3021

ZP_01797281.1

Haemophilus influenzae R3021

ZP_10073638.1

Haemophilus paraphrohaemolyticus HK411

ZP_02479394.1

Haemophilus parasuis 29755

YP_002475717.l

Haemophilus parasuis SH0165

ZP_09377516.l

Hafnia alvei ATCC 51873

YP_003993926.1

Halanaerobium hydrogeniformans

yp_004445529.1

Haliscomenobacter hydrossis DSM llOO

yp_006180809.1

Halobacillus halophilus DSM 2266

ZP_09188055.1

Halomonas boliviensis LCl

yp_003897727.1

Halomonas elongata DSM 2581

yp_003897090.l

Halomonas elongata DSM 2581

ZP_09738126.l

Helcococcus kunzii ATCC 51366

ZP_07805046.1

Helicobacter cinaedi CCUG 18818

ZP_04809499.1

Helicobacter pullorum MIT 98-5489

ZP_04808991.l

Helicobacter pullorum MIT 98-5489

YP_005785121.1

Helicobacter pylori 83

yp_001681197.1

Heliobacterium modesticaldum lcel

yp_001099394.l

Herminiimonas arsenicoxydans

yp_005511208.l

Hydrogenobacter thermophilus TK-6

ZP_09999130.1

lmtechella halotoferans Kl

yp_002128512.l
lodobacteriophage phiPLPE

ZP_09154646.l

Johnsonella ignava ATCC 51276

yp_003162220.l

Jonesia denitrificans DSM 20603

EHS95796.1

Klebsiella oxytoca 10-5243

EHT14033.1

Klebsiella oxytoca 10-5250

AEU09829.1

Klebsiella pneumoniae

yp_002238960.l

Klebsiella pneumoniae 342

YP_005953460.1

Klebsiella pneumoniae KCTC 2242

YP_005953897.1

Klebsiella pneumoniae KCTC 2242

YP_001335044.1

Klebsiella pneumoniae subsp. pneumoniae MGH 78578

ZP_06016835.1

Klebsiella pneumoniae subsp. rhinoscleromatis ATCC 13884

ZP_06552348.1

Klebsiella sp. 1_1_55

ZP_08306099.1

Klebsiella sp. MS 92-3

yp_003438695.1

Klebsiella variicola At-22

ZP_02163271.1

Kordia algicida OT-1

ZP_08616825.l
Lachnospiraceae bacterium _4_56FAA

ZP_08339829.l
Lachnospiraceae bacterium 2_1_46FAA

ZP_08336670.1
Lachnospiraceae bacterium 3_1_46FAA

ZP_08604142.1
Lachnospiraceae bacterium 3_1_57FAA_CT1

ZP_08149693.1
Lachnospiraceae bacterium 4_1_37FAA

ZP_08603145.1
Lachnospiraceae bacterium 5_1_57FAA

ZP_08331597.1
Lachnospiraceae bacterium 6_1_63FAA

ZP_09532735.1
Lachnospiraceae bacterium 7_1_58FAA

ZP_08334226.1
Lachnospiraceae bacterium 9_1_43BFAA

ZP_09521413.1
Lachnospiraceae bacterium ACC2

VP_004581188.1

Lacinutrix sp. 5H-3-7-4

VP_004580596.1

Lacinutrix sp. 5H-3-7-4

VP_005858762.1

Lactobacillus casei BD-11

VP_005851623.1

Lactobacillus delbrueckii subsp. bulgaricus 2038

VP_005848993.1

Lactobacillus fermentum CECT 5716

VP_005850725.1

Lactobacillus helveticus H10

VP_005862099.1

Lactobacillus johnsoni i DPC 6026

VP_004890503.1

Lactobacillus plantarum WCFSl

VP_00586S109.1

Lactobacillus rhamnosus GG

ZP_08564335.1

Lactobacillus ruminis SPM0211

VP_396489.1

Lactobacillus sakei subsp. sakei 23K

VP_005863385.1

Lactobacillus salivarius CECT 5713

ZP_08890725.1

Lactobacillus sp. 7_1_47FAA

VP_005869785.1

Lactococcus garvieae Lg2

EHE92415.1

Lactococcus lactis subsp. cremoris CNCM 1-1631

NP_996684.1

Lactococcus phage phiLC3

ABD63859.1

Lactococcus phage phismq86

VP_00279S496.1

Laribacter hongkongensis HLHK9

VP_003998593.1

Leadbetterella byssophila DSM 17132

VP_003997999.1

Leadbetterella byssophila DSM 17132

CAC33455.1

Legionella pneumophila

VP_003163767.1

Leptotrichia buccalis C-1013-b

ZP_06011746.1

Leptotrichia goodfellowii F0264

VP_005174459.1

Leuconostoc mesenteroides subsp. mesenteroides J18

VP_004704993.1

Leuconostoc sp. C2

NP_471091.1

Listeria innocua Clipl 262

ZP_07873574.1

Listeria ivanovii FSL F6-596

ZP_05289346.1

Listeria monocytogenes FSL F2-515

ZP_05297269.1

Listeria monocytogenes FSLJ2-003

ZP_05299340.1

Listeria monocytogenes FSL J2-003

ZP_07075805.1

Listeria monocytogenes FSL N1-017

EEW20557.1

Listeria monocytogenes FSL R2-503

EEW2026S.1

Listeria monocytogenes FSL R2-S03

ZP_00235039.1

Listeria monocytogenes str. 1/2a F6854

VP_003464587.1

Listeria seeligeri serovar 1/2b str. SLCC3954

ZP_04979099.1

Mannheimia haemolytica PHL213

ZP_09732369.1

Megamonas funiformis VIT 11815

YP_004456677.1

Melissococcus plutonius ATCC 35311

YP_005318817.1

Melissococcus plutonius DAT561

BAB53634.1

Mesorhizobium Ioli MAFF303099

YP_002502020.1

Methylobacterium nodulans ORS 2060

YP_002501391.1

Methylobacterium nodu/ans ORS 2060

YP_002497906.1

Methylobacterium nodulans ORS 2060

YP_002498798.1

Methylobacterium nodulans ORS 2060

ZP_09899678.1

Methylomicrobium album BG8

YP_004915903.1

Methylomicrobium alcaliphilum

yp_004512397.1

Methylomonas methanica MC09

CA090389.1

Microcyst'1s aeruginosa PCC 7806

ZP_07908870.1

Mobiluncus curtisii ATCC 51333

EGE24382.1

Moraxella catarrhalis 101P3081

EGE19718.1

Moraxella catarrhalis 8Cl

yp_004789848.1

Muricauda ruestringensis DSM 13258

yp_004789429.1

Muricauda ruestringensis DSM 13258

EIC64366.1

Mycobacterium abscessus M93

EIC70403.1

Mycobacterium abscessus M94

yp_001852190.1

Mycobacterium marinum M

8AH69619.1

Mycoplasma fermentans PG18

yp_005888312.1

Mycoplasma hyopneumoniae 168

yp_003856575.1

Mycoplasma hyorhinis HU8-l

yp_005905159.1

Mycoplasma hyorhinis MCLD

ZP_09524021.l

Myroides adoratimimus CCUG 10230

EH012592.1

Myroides adoratimimus CIP 101113

YP_004666545.1

Myxacoccus fulvus HW-1

VP_004669149.1

Myxacoccus fulvus HW-1

YP_630241.1

Myxococcus xanthus DK 1622

ZP_08247669.1

Neisseria bacilliformis ATCC 8AA-1200

ZP_03719260.1

Neisseria flavescens NRL30031/H210

yp_005889683.1

Neisseria gonorrhoeae TCDC-NG08107

yp_004048639.1

Neisseria lactamica 020-06

C8X21356.1

Neisseria lactamica Y92-1009

yp_005892581.1

Neisseria meningitidis WUE 2594

yp_005006679.1

Niastella koreensis GR20-10

yp_004168204.1

Nitratifractor salsuginis DSM 16511

ZP_09514368.1

Oceanicola sp. Sl24

VP_005092407.1

Oceanimonas sp. GKl

yp_005093273.1

Oceanimonas sp. GKl

NP_693974.1

Oceanobacillus iheyensis HTE831

ZP_09641960.1

Odoribacter laneus YIT 12061

ZP_09326776.1

Oribacterium sp. ACBl

yp_004988339.1

Owenweeksia hongkongensis DSM 17368

yp_004988588.1

Owenweeksia hongkongensis DSM 17368

ZP_09077613.1

Paenibacillus elgii 869

ZP_09079484.1

Paenibacillus elgii 869

ZP_09071954.1

Paenibacillus larvae subsp. larvae 8-3650

ZP_09069307.1

Paenibacillus larvae subsp. larvae B-3650

ZP_09069904.1

Paenibacillus larvae subsp. larvae 8-3650

ZP_09072245.1

Paenibacillus larvae subsp. larvae 8-3650

ZP_02327781.1

Paenibacillus larvae subsp. larvae 8RL-230010

ZP_02326141.1

Paenibacillus larvae subsp. larvae 8RL-230010

YP_006190082.1

Paenibacillus mucilaginasus K02

yp_003010343.1

Paenibacillus sp. JDR-2

yp_003013704.1

Paenibacillus sp. JDR-2

ZP_04854710.1

Paenibacillus sp. oral taxon 786 str. D14

yp_005073546.1

Paenibacillus terrae HPL-003

VP_003519587.1

Pantoea ananatis LMG 20103

VP_005993583.1

Pantoea ananatis PA13

yp_005994280.1

Pantoea ananatis PA13

ZP_07380439.1

Pantoea sp. aB

yp_003931279.1

Pantoea vagans C9-1

ZP_03477183.1

Parabacteroides johnsonii DSM 18315

ZP_03475832.1

Parabacteroides johnsoni i DSM 18315

ZP_02033987.1

Parabacteroides merdae ATCC 43184

ZP_02032292.1

Parabacteroides merdae ATCC 43184

ZP_06299504.1

Parachlamydia acanthamoebae str. Hall's coccus

yp_917501.1

Paracoccus denitrificans PD1222

ZP_02094048.1

Parvimonas micra ATCC 33270

YP_051100.1

Pectobacterium atrosepticum SCRI 1043

ZP_07367149.1

Pediococcus acidilactici DSM 20284

yp_005005652.1

Pediococcus claussenii ATCC BAA-344

yp_004272912.1

Pedobacter saltans DSM 12145

yp_901551.1

Pelobacter propionicus DSM 2379

YP_903200.1

Pelobacter propionicus DSM 2379

ZP_07400314.1

Peptoniphilus duerdenii ATCC BAA-1640

yp_002731548.1

Persephonella marina EX-Hl

NP_461568.2
Phage Gifsy-1

yp_005352506.1

Photobacterium damselae subsp. damselae

ZP_06157475.1

Photobacterium damselae subsp. damselae CIP 102761

yp_132232.1

Photobacterium profundum SS9

NP_930169.1

Photorhabdus luminescens subsp. laumondii TTOl

YP_004270153.1

Planctomyces brasiliensis DSM 5305

yp_001155602.1

Polynucleobacter necessarius subsp. asymbioticus QLW-Pl DMWA-1

ZP_09103791.1

Prevotel/ a histico/a F0411

ZP_09554100.1

Prevotella maculosa OT 289

ZP_09590809.1

Prevote/la micans F0438

ZP_09120679.1

Prevotel/a sp. oral taxon 302 str. F0323

ZP_03840270.1

Proteus mirabi/is ATCC 29906

ZP_03805638.1

Proteus penneri ATCC 35198

ZP_03804770.1

Proteus penneri ATCC 35198

ZP_03318967.1

Providencia alca/ifaciens DSM 30120

ZP_03320274.1

Providencia alcalifaciens DSM 30120

AAM08027.1

Providencia rettgeri

ZP_06124041.2

Providencia rettgeri DSM 1131

EFE55836.1

Providencia rettgeri DSM 1131

yp_006217717.1

Providencia stuartii MRSN 2154

YP_661287.1

Pseudoalteromonas atlantica T6c

YP_663733.1

Pseudoalteromonas at/antica T6c

ZP_09236879.1

Pseudoalteromonas sp. BSi20439

ZP_09234821.1

Pseudoa/teromonas sp. BSi20439

ZP_09235053.1

Pseudoalteromonas sp. BSi20439

ZP_09243850.1

Pseudoalteromonas sp. BSi20495

YP_004846059.1

Pseudogulbenkiania sp. NH8B

ACD38903.1

Pseudomonas aeruginosa

ACD38827.1

Pseudomonas aeruginosa

ZP_07797103.1

Pseudomonas aeruginosa 39016

YP_005975666.1

Pseudomonas aeruginosa M18

YP_004355228.1

Pseudomonas brassicacearum subsp. brassicacearum NFM421

YP_005208095.1

Pseudomonas f/uorescens F113

yp_348340.1

Pseudomonas fluorescens PI0-1

yp_002872416.1

Pseudomonas fluorescens SBW2S

yp_004380540.1

Pseudomonas mendocina NK-01

YP_005098041.1

Pseudomonas phage phi297

yp_005930618.1

Pseudomonas putida BIRD-1

yp_001667964.1

Pseudomonas putida GB-1

yp_005938868.1

Pseudomonas stutzeri DSM 4166

YP_005937536.1

Pseudomonas stutzeri DSM 4166

EGH87883.1

Pseudomonas syringae pv. lachrymans str. M30131S

EGH59138.1

Pseudomonas syringae pv. maculicola str. ES4326

yp_274842.1

Pseudomonas syringae pv. phaseolicola 1448A

EGH43274.1

Pseudomonas syringae pv. pisi str. 17048

yp_235897.1

Pseudomonas syringae pv. syringae B728a

YP_264268.1

Psychrobacter arcticus 273-4

yp_001280242.1

Psychrobacter sp. PRwf-1

yp_942451.1

Psychromonas ingrahamii 37

yp_944546.1

Psychromonas ingrahamii 37

yp_005199476.1

Rahnella aquatilis CIP 78.6S = ATCC 33071

yp_006029734.1

Ralstonia solanacearum Po82

yp_002281965.1

Rhizobium leguminosarum bv. trifolii WSM2304

yp_005087203.1

Rhodococcus phage REQ3

yp_004011824.1

Rhodomicrobium vannielii ATCC 17100

yp_006017652.1

Riemerella anatipestifer RA-GD

yp_004838017.1

Roseburia hominis A2-183

ZP_03755701.1

Roseburia inulinivorans DSM 16841

ZP_03166627.1

Ruminococcus lactaris ATCC 29176

ZP_04855253.1

Ruminococcus sp. S_1_39B_FAA

yp_004655025.1

Runella slithyformis DSM 19S94

YP_004653638.1

Runella slithyformis DSM 19S94

yp_527033.1

Saccharophagus degradans 2-40

yp_001571105.1

Salmonella enterica subsp. arizonae serovar 62: z4, z23: — str. RSK2980

EHC38944.1

Salmonella enterica subsp. enterica serovar Gaminara str. A4-S67

ZP_ 03220723.1

Salmonella enterica subsp. enterica serovar Javiana str. GA_MM04042433

EDZ19028.1

Salmonella enterica subsp. enterica serovar Kentucky str. CDC 191

EHC73330.1

Salmonella enterica subsp. enterica serovar Mississippi str. A4-633

EHL46421.1

Salmonella enterica subsp. enterica serovar Montevideo str. SARB30

EHC90429.1

Salmonella enterica subsp. enterica serovar Rubislaw str. A4-6S3

EHC91146.1

Salmonella enterica subsp. enterica serovar Senftenberg str. A4-S43

ZP_03338172.1

Salmonella enterica subsp. enterica serovar Typhi str. 404ty

ZP_03340438.1

Salmonella enterica subsp. enterica serovar Typhi str. 404ty

ZP_03341127.1

Salmonella enterica subsp. enterica serovar Typhi str. 404ty

ZP_06538095.1

Salmonella enterica subsp. enterica serovar Typhi str. AG3

AAC26069.l

Salmonella enterica subsp. enterica serovar Typhimurium

EHY70960.1

Salmonella enterica subsp. houtenae str. ATCC BAA-1581

yp_005098013.1

Salmonella phage SPNlS

yp_005321079.1

Saprospira grandis str. Lewin

yp_005323878.1

Saprospira grandis str. Lewin

yp_003308034.1

Sebaldella termitidis ATCC 33386

yp_003657357.1

Segniliparus rotundus DSM 4498S

EHG25612.1

Selenomonas noxia F0398

yp_005433748.1

Selenomonas ruminantium subsp. lactilytica TAM6421

ZP_06640797.1

Serratia odorifera DSM 4582

VP_006024370.1

Serratia sp. AS13

ZP_08039150.1

Serratia symbiotica str. Tucson

yp_927263.1

Shewanella amazonensis SB2B

VP_006020740.1

Shewanella baltica BA175

VP_005272800.1

Shewanella baltica 05678

VP_001093802.1

Shewanella Joihica PV-4

NP_718112.1

Shewanella oneidensis MR-1

NP_717301.1

Shewanella oneidensis MR-1

VP_002312664.1

Shewanella piezotolerans WP3

yp_002311778.1

Shewanella piezotolerans WP3

VP_006011723.1

Shewanella putrefaciens 200

VP_001182934.1

Shewanella putrefaciens CN-32

VP_001473852.1

Shewanella sediminis HAW-EB3

VP_001473225.1

Shewanella sediminis HAW-EB3

VP_003556874.1

Shewanella violacea DSS12

VP_003557838.1

Shewanella violacea DSS12

YP_001760744.1

Shewanella woodyi ATCC 51908

AAF28115.l,

Shigella dvsenteriae

AF153317_10

ZP_07683610.1

Shigella dysenteriae 1617

EFW50178.1

Shigella dysenteriae CDC 74-1112

ZP_04454959.1

Shuttleworthia satelles DSM 14600

y p_004662889.1

Simkania negevensis Z

VP_001327594.1

Sinorhizobium medicae WSM419

yp_001327785.1

Sinorhizobium medicae WSM419

VP_004549676.1

Sinorhizobium meliloti AK83

VP_004548149.1

Sinorhizobium meliloti AK83

EHK78038.l

Sinorhizobium meliloti CCNWSX0020

VP_005719596.1

Sinorhizobium meliloti SMll

YP_455377.1

Sodalis glossinidius str. ‘morsitans'

VP_516203.l

Sodalis phage phiSGl

YP_001616277.1

Sorangium cellulosum ‘So ce 56’

YP_004247313.1

Sphaerochaeta globus str. Buddy

YP_005061390.1

Sphaerochaeta pleomorpha str. Grapes

VP_004317691.1

Sphingobacterium sp. 21

yp_004315662.1

Sphingobacterium sp. 21

YP_005474943.1

Spirochaeta africana DSM 8902

VP_004411234.1

Spirochaeta coccoides DSM 17374

YP_003804225.1

Spirochaeta smaragdinae DSM 11293

VP_003803689.1

Spirochaeta smaragdinae DSM 11293

ZP_08680759.1

Sporosarcina newyorkensis 2681

ZP_06324416.1

Staphylococcus aureus subsp. aureus D139

VP_005739407.1

Staphylococcus aureus subsp. aureus JKD6159

YP_253435.1

Staphylococcus haemolyticus JCSC143S

YP_006015496.1

Staphylococcus pseudintermedius ED99

YP_003952450.1

Stigmatella aurantiaca DW4/3-1

yp_006035100.1

Streptococcus gallolyticus subsp. gallolyticus ATCC 43143

ZP_07463816.1

Streptococcus gallolyticus subsp. gallolyticus TX20005

EGV02688.1

Streptococcus infantis SK970

EHG11465.1

Streptococcus intermedius F0395

NP_722442.1

Streptococcus mutans UA159

VP_004479394.1

Streptococcus parauberis KCTC 11537

CBW39270.1

Streptococcus phage 2167

CBW39216.1

Streptococcus phage 8140

VP_596543.1

Streptococcus phage 9429.2

ABD48929.1

Streptococcus phage M102

VP_002995484.l

Streptococcus phage M102

CBW38953.1

Streptococcus phage V22

EHE64346.1

Streptococcus pneumoniae EU-NPOl

EHE76000.1

Streptococcus pneumoniae GAI 1426

VP_005412494.1

Streptococcus pyogenes MGAS1882

VP_001128217.1

Streptococcus pyogenes str. Manfredo

CCB95242.1

Streptococcus salivarius JIM8777

ZP_03625981.1

Streptococcus suis 89/1591

yp_00608S650.1

Streptococcus suis A7

yp_006078894.1

Streptococcus suis SS12

EHE89971.1

Streptococcus thermophilus CNCM 1-1630

ZP_09342658.1

Subdoligranulum sp. 4_3_54A2FAA

yp_005014978.1

Tannerella forsythia ATCC 43037

yp_005015967.1

Tannerella forsythia ATCC 43037

ZP_09337816.1

Tannerella sp. 6_1_58FAA_CT1

ZP_09337420.1

Tannerella sp. 6_1_58FAA_CT1

yp_004874525.1

Tavlorella asinigenitalis MCE3

YP_003477226.1

Thermoanaerobacter italicus Ab9

yp_004150652.1

Thermovibrio ammonificans HB-1

ZP_03497413.1

Therm us aquaticus Y51MC23

YP_313909.1

Thiobacillus denitrificans ATCC 25259

ZP_08768668.1

Thiocapsa marina 5811

ZP_08772750.1

Thiocapsa marina 5811

ZP_08926729.1

Thiocystis violascens DSM 198

ZP_08824405.1

Thiorhodococcus drewsii AZl

ZP_08822326.1

Thiorhodococcus drewsii AZl

ZP_08825220.1

Thiorhodococcus drewsii All

ZP_09809048.1

Thiorhodovibrio sp. 970

ZP_09865744.1

Thiorhodovibrio sp. 970

ZP_09865982.1

Thiorhodovibrio sp. 970

ZP_09809289.1

Thiorhodovi brio sp. 970

yp_004438805.1

Treponema brennaborense DSM 12168

yp_005224087.1

Treponema pallidum subsp. pallidum DAL-1

yp_722968.1

Trichodesmium erythraeum IMS101

ZP_02971366.1

Ureaplasma parvum serovar 6 str. ATCC 27818

EDX53145.1

Ureaplasma urealyticum serovar 12 str. ATCC 33696

ZP_03771988.1

Ureaplasma urea/yticum serovar 8 str. ATCC 27618

ZP_01237386.1

Vibrio angustum S14

ZP_07741429.1

Vibrio caribbenthicus ATCC BAA-2122

ZP_01947910.1

Vibrio cholerae 1587

YP_005633953.1

Vibrio cholerae LMA3984-4

YP_005633301.1

Vibrio cholerae LMA3984-4

AAX89425.1

Vibrio cholerae non-Ol/non-0139

ABY28344.1

Vibrio cholerae 0139

ZP_05880433.1

Vibrio furnissii CIP 102972

yp_004993437.1

Vibrio furnissii NCTC 11218

ZP_06177392.1

Vibrio harvevi 1DA3

ZP_06174696.1

Vibrio harvevi 1DA3

VP_002417106.1

Vibrio splendidus LGP32

VP_004238525.1

Weeksella virosa DSM 16922

VP_004726213.1

Weissella koreensis KACC 15510

VP_001418489.1

Xanthobacter autotrophicus Pv2

yp_003467094.1

Xenorhabdus bovienii SS-2004

yp_003662329.1

Xenorhabdus nematophila ATCC 19061

yp_003325187.1

xvlanimonas cellulosilvtica DSM 15894

AAF84457.1,

Xvlella fastidiosa 9a5c

AE003991_9

ZP_00683037.1

Xvlella fastidiosa Ann-1

ZP_00682624.1

Xvlella fastidiosa Ann-1

ZP_00683132.1

Xvlella fastidiosa Ann-1

VP_006001389.1

Xvlella fastidiosa subsp. fastidiosa GB514

yp_006005235.1

Versinia enterocolitica subsp. palearctica Vll

yp_070310.1

Versinia pseudotuberculosis IP 32953

yp_001101797.1

Yersinia ruckeri

yp_004739144.1

Zobellia galactanivorans

yp_004738450.1

Zobellia galactanivorans

F9ZNV9

Acidithiobacillus caldus

C6AM23

Aggregatibacter aphrophilus

A6TRD8

Alkaliphilus metalliredigens

D3RWF4

Allochromatium vinosum

B9MNF3

Anaerocellum thermophilum

C6ACZ8

Bartonella grahamii

A91YA2

Bartonella tribocorum

A91T52

Bartonella tribocorum

E8MTD2

Bifidobacterium longum subsp. infantis

A3NMOO

Burkholderia pseudomallei

E4QCOO

Caldicellulosi ruptor hvdrothermalis

E4S5A7

Caldicellulosiruptor kristjanssonii

E4SEV3

Caldicellulosiruptor kronotskyensis

A4XJG5

Caldicellulosiruptor saccharolvticus

A4XMN9

Caldicellulosiruptor saccharolvticus

A7HOW7

Campylobacter curvus

A7H394

Campylobacter jejuni subsp. doylei

Q3ABH5

Carboxydothermus hydrogenoformans

D9SWGO

Clostridium cellulovorans

Q18AA7

Clostridium difficile

H8MHN3

Corallococcus coralloides

GOHB56

Corynebacterium variabile

C7F4E8

cvanophage pSS2

F6AU64

Delftia sp.

CG19468

Drosophila melanogaster

GOEOR4

Enterobacter aerogenes

D5CJK9

Enterobacter cloacae subsp. cloacae

A4W7K6

Enterobacter sp.

81XAU6

Escherichia coli

87LNE9

Escherichia fergusonii

H8L634

Frateuria aurantia

Q5FN39

Gluconobacter oxydans

B8F617

Haemophilus parasuis serovar 5

E4RNA4

Halanaerobium sp.

P43351

Homo sapiens

D3DGM9

Hydrogenobacter thermophilus

Q9MBV8

Lactotoccus phage ul16.2

C1D7P7

Laribacter hongkongensis

Q9AKZO

Legionella pneumophila

Q926Al

Listeria innocua serovar 6a

Q9T172

Listeria phage A118

F3YBJO

Melissococcus plutonius

B2HPl3

Mycobacterium marinum

F8CQD8

Myxococcus fulvus

Q1DAT2

Myxococcus xanthus

E7BFDS

Neisseria meningitidis serogroup A

C6CX43

Paenibacillus sp.

A1B8Gl

Paracoccus denitrificans

A1AQ73

Pelobacter propionicus

COQSA2

Persephonella marina

Q7N2Y8

Photorhabdus luminescens

Q7N2Y8

Photorhabdus luminescens subsp. laumondii

B4EWRS

Proteus mirabilis

C3KA14

Pseudomonas fluorescens

ASWFSl

Psychrobacter sp.

Q984J6

Rhizobium loti

YML032

Saccharomyces cerevisiae

B4TDT1

Salmonella heidelberg

A41VH9

Salmonella newport

A9MSD6

Salmonella paratyphi B

Q8Z7Y3

Salmonella typhi

H6L1F4

Saprospira grandis

E6XGY4

Shewanella putrefaciens

AlRHSl

Shewanella sp.

Q32GM7

Shigella dysenteriae serotype 1

F6DWY3

Sinorhizobium meliloti

F7X3Dl

Sinorhizobium meliloti

Q2NSA3

Sodalis glossinidius

E1R1F4

Spirochaeta smaragdinae

E1R3KO

Spirochaeta smaragdinae

Q08VK7

Stigmatella aurantiaca

FSZL14

Streptococcus parauberis

ClCGll

Streptococcus pneumoniae

EOTQLO

Streptococcus pneumoniae

Q9A029

Streptococcus pyogenes serotype Ml

Q1CQT2

Streptococcus pyogenes serotype M12

A2RDQ6

Streptococcus pyogenes serotype MS

F8LPH2

Streptococcus salivarius

E8T306

Thermovibrio ammonificans

Q8KQWO

Vibrio cholerae

C3NU24

Vibrio cholerae serotype 01

B7VNT1

Vibrio splendidus

D3V014

Xenorhabdus bovienii

D1BWP7

Xylanimonas cellulosilytica

Q87CQ1

Xylella fastidiosa

Q9PCV7

Xylella fastidiosa

Q66Bl7

Yersinia pseudotuberculosis

TABLE 7

Single Stranded DNA-Binding Proteins.

SS DNA-BINDING PROTEINS

gi|802137314|emb|CQR83440.1|: 39-216 single-stranded DNA-binding

protein [Escherichia coli K-12]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 11)

gi|115515464|gb|ABJ03539.1|: 20-197 single strand DNA-binding protein

[Escherichia coli APEC O1]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 12)

gi|91075172|gb|ABE10053.1|: 22-199 single strand DNA-binding protein

[Escherichia coli UTI89]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 13)

gi|446090450|ref|WP_000168305.1|: 1-178 MULTISPECIES: ssDNA-binding

protein [Proteobacteria]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 14)

gi|446090454|ref|WP_000168309.1|: 1-178 MULTISPECIES: ssDNA-binding

protein [Escherichia]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNV

GGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 15)

gi|446090447|ref|WP_000168302.1|: 1-178 ssDNA-binding protein

[Shigella flexneri]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNKFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 16)

gi|446079209|ref|WP_000157064.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASKGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 17)

gi|953799395|ref|WP_058036055.1|: 1-178 single-stranded DNA-binding

protein [Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYLEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 18)

gi|766978151|ref|WP_044868983.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSAQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 19)

gi|446090443|ref|WP_000168298.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAAGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 20)

gi|851928251|ref|WP_048220840.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGHDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 21)

gi|754840036|ref|WP_042201710.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQSGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 22)

gi|896026319|ref|WP_049086626.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGSWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 23)

gi|921979423|ref|WP_053271110.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGSAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 24)

gi|585361910|ref|WP_024243502.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGSNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 25)

gi|692999009|ref|WP_032185683.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGSNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 26)

gi|545166587|ref|WP_021521173.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNSGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 27)

gi|446090455|ref|WP_000168310.1|: 1-178 MULTISPECIES: ssDNA-binding

protein [Escherichia]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQSGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 28)

gi|585341793|ref|WP_024223419.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSTPAAPSNEPPMDFDDDIPF (SEQ ID NO: 29)

gi|757739421|ref|WP_042963455.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQSQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 110)

gi|486058717|ref|WP_001508182.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSCGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 30)

gi|446090457|ref|WP_000168312.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLTGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMXMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 31)

gi|723112103|ref|WP_033557595.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVVSEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 32)

gi|693064509|ref|WP_032230375.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGVQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 33)

gi|844746903|ref|WP_047928938.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGDAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 34)

gi|446090446|ref|WP_000168301.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQDGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 35)

gi|735676636|ref|WP_034167446.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYITEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 36)

gi|558576622|gb|AHA68760.1|: 22-199 Single-strand DNA bindingprotein

[Shigella dysenteriae 1617]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQLQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 37)

gi|974633789|ref|WP_059220463.1|: 1-178 single-stranded DNA-binding

protein [Escherichia albertii]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGLDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 38)

gi|961657168|emb|CRL87896.1|: 1-178 Single-stranded DNA-binding

protein [Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLCKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 39)

gi|446090440|ref|WP_000168295.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGLDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 40)

gi|446090449|ref|WP_000168304.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQPAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 41)

gi|446090444|ref|WP_000168299.1|: 1-178 MULTISPECIES: ssDNA-binding

protein Enterobacteriaceae]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQLQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 42)

gi|585353006|ref|WP_024234614.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKDQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSTPAAPSNEPPMDFDDDIPF (SEQ ID NO: 43)

gi|823645439|ref|WP_047090144.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRLQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 44)

gi|545298006|ref|WP_021578210.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQLQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 45)

gi|446090456|ref|WP_000168311.1|: 1-178 MULTISPECIES: ssDNA-binding

protein [Escherichia]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQSGGAPTGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 46)

gi|487374574|ref|WP_001647924.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQGYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 47)

gi|485855265|ref|WP_001456829.1|: 1-178 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEGASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 48)

gi|974673892|ref|WP_059258066.1|: 1-178 single-stranded DNA-binding

protein [Escherichia albertii]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSLVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQSGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 49)

gi|446090442|ref|WP_000168297.1|: 1-178 ssDNA-binding protein

[Escherichia albertii]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSEFWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQSGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 50)

gi|446090451|ref|WP_000168306.1|: 1-188 ssDNA-binding protein

[Escherichia coli]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ

ID NO: 111)

gi|780016555|ref|WP_045438572.1|: 1-177 MULTISPECIES: ssDNA-binding

protein [Citrobacter]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGNA

GGGQQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 51)

gi|835333245|ref|WP_047459655.1|: 1-178 ssDNA-binding protein

[Citrobacter koseri]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGAPAGGNM

GGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 52)

gi|558738441|gb|EST86567.1|: 1-171 single-stranded DNA-binding

protein [Escherichia coli ECC-1470]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMD (SEQ ID NO: 53)

gi|501084104|ref|WP_012134613.1|: 1-178 ssDNA-binding protein

[Citrobacter koseri]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGVPAGGNM

GGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 54)

gi|673534660|emb|CDZ85363.1|: 1-178 single-stranded DNA-binding

protein [Citrobacter koseri]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDKYITEVVVNVGGTMQMLGGRQGGGAPAGGNM

GGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 55)

gi|446090452|ref|WP_000168307.1|: 1-171 MULTISPECIES: ssDNA-binding

protein [Shigella]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIG

GGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 56)

gi|489923275|ref|WP_003826621.1|: 1-174 MULTISPECIES: ssDNA-binding

protein Enterobacteriaceae]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 57)

gi|817709260|ref|WP_046671365.1|: 1-174 MULTISPECIES: ssDNA-binding

protein [Citrobacter freundii complex]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGGQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 58)

gi|489121902|ref|WP_003031723.1|: 1-174 MULTISPECIES: ssDNA-binding

protein [Citrobacter]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGEQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 59)

gi|493739129|ref|WP_006688290.1|: 1-174 ssDNA-binding protein

[Citrobacter youngae]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 60)

gi|754956873|ref|WP_042312982.1|: 1-174 single-stranded DNA-binding

protein [Citrobacter werkmanii]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSNEPSMDFDDDIPF (SEQ ID NO: 61)

gi|851983070|ref|WP_048241790.1|: 1-174 ssDNA-binding protein

[Citrobacter sp. MGH109]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRLQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 62)

gi|507084615|ref|WP_016155359.1|: 1-175 MULTISPECIES: ssDNA-binding

protein Enterobacteriaceae]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 63)

gi|754934450|ref|WP_042291190.1|: 1-174 MULTISPECIES: ssDNA-binding

protein [Citrobacter]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGAPAGGQQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 64)

gi|489956977|ref|WP_003860284.1|: 1-174 MULTISPECIES: ssDNA-binding

protein [Proteobacteria]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 65)

gi|390676734|gb|EIN52819.1|: 1-155 single-stranded DNA-binding protein

[Escherichia coli PA5]

MPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLEGKLAEVASEYLRKGSQVYIEGQLRTRK

WTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGG

AQSRPQQSAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 66)

gi|695799260|ref|WP_032713447.1|: 1-174 ssDNA-binding protein

[Enterobacter aerogenes]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEIVVNVGGTMQMLGGRQGGGAPASGGQQ

QGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 67)

gi|695739375|ref|WP_032665749.1|: 1-176 ssDNA-binding protein

[Enterobacter cloacae]

MASKGVNKVILVGNLGQDPEVRYLPSGGAVCSVTLATSESWRDKATGELKEQTEWHRVVLFGKLA

EVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGAPTGGSQNQ

QQGGWGRHQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDLDDDIPF (SEQ ID NO: 68)

gi|895840578|ref|WP_048957472.1|: 1-174 ssDNA-binding protein

[Enterobacter cloacae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGAEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGSQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 69)

gi|959987279|gb|ALR75018.1|: 1-178 single-stranded DNA-binding

protein [Klebsiella sp. G5]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGNM

GGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 70)

gi|896091886|ref|WP_049127706.1|: 1-175 ssDNA-binding protein

[Klebsiella oxytoca]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQQGASAPAGGG

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 71)

gi|745784477|ref|WP_039077898.1|: 1-178 MULTISPECIES: ssDNA-binding

protein [Enterobacteriaceae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGAEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGNM

GGGQGQQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 72)

gi|503133400|ref|WP_013368061.1|: 1-178 ssDNA-binding protein

[Enterobacter lignolyticus]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGASAGGNM

GGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF(SEQ ID NO: 73)

gi|727165443|ref|WP_033636191.1|: 1-176 ssDNA-binding protein

[Serratia marcescens]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEQKEKTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGSLQTRKWQDQSGQDRYTTEIVVNVGGTMQMLGGRQGGGAPAGQSAG

GQSGWGQPQQPQGGNQFSGGQQQSRPAQNSAPATSNEPPMDFDDDIPF (SEQ ID NO: 74)

gi|782727713|ref|WP_045620928.1|: 1-174 MULTISPECIES: ssDNA-binding

protein [Enterobacter cloacae complex]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGSGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 75)

gi|695651336|ref|WP_032619399.1|: 1-176 MULTISPECIES: ssDNA-binding

protein [Enterobacter cloacae complex]

MASKGVNKVILVGNLGQDPEVRYLPSGGAVCSVTLATSESWRDKATGELKEQTEWHRVVLFGKLA

EVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGSQN

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF(SEQ ID NO: 76)

gi|490199293|ref|WP_004097799.1|: 1-175 MULTISPECIES: ssDNA-binding

protein [Enterobacteriaceae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQQGAGAPAGGG

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 77)

gi|490994755|ref|WP_004856483.1|: 1-175 MULTISPECIES: ssDNA-binding

protein [Enterobacteriaceae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTILIVVNVGGTMQMLGGRQQGAGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 78)

gi|980964768|ref|WP_059385087.1|: 1-176 single-stranded DNA-binding

protein [Enterobacter cloacae]

MASKGVNKVILVGNLGQDPEVRYLPSGSAVCSVTLATSESWRDKATGELKEQTEWHRVVLFGKLA

EVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGSQN

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 79)

gi|896015843|ref|WP_049079199.1|: 1-175 ssDNA-binding protein

[Klebsiella oxytoca]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQQGAGAPAGGG

QQQGGWGQPQQPQGGNQYSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 80)

gi|490229479|ref|WP_004127826.1|: 1-175 ssDNA-binding protein

[Klebsiella oxytoca]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQQGAGAPAGGG

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQTPAAPSNEPPMDFDDDIPF (SEQ ID NO: 81)

gi|727291496|ref|WP_033749160.1|: 1-184 MULTISPECIES: ssDNA-binding

protein [Pantoea]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGENKEITEWHRVVLFGKLA

EVAGEYLRKGSQVYIEGQLRTRKWQDQGGQDRYTTEVVVNVGGTMQMLGGRQQGGASAGGAPM

GGGQQSGGNNNGWGQPQQPQGGNQFSGGAQSRPQPQSAPASNNNEPPMDFDDDIPF (SEQ ID

NO: 82)

gi|950093073|ref|WP_057172980.1|: 1-175 single-stranded DNA-binding

protein [Klebsiella oxytoca]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQQGAGAPAGGG

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNETPMDFDDDIPF (SEQ ID NO: 83)

gi|896291109|ref|WP_049273747.1|: 1-176 MULTISPECIES: ssDNA-binding

protein [Enterobacteriaceae]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEQKEKTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGSLQTRKWQDQSGQDRYTTEIVVNVGGTMQMLGGRQGGGAPAGQSAG

GQGGWGQPQQPQSGNQFSGGQQQSRPAQNSAPATSNEPPMDFDDDIPF (SEQ ID NO: 84)

gi|556473803|ref|WP_023325414.1|: 1-174 ssDNA-binding protein

[Klebsiella pneumoniae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKHTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 85)

gi|983342195|ref|WP_060522659.1|: 1-171 single-stranded DNA-binding

protein [Klebsiella pneumoniae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWH

RVVLFGKLAEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGG

GAPAGGGQQQGGWGQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 86)

gi|490253751|ref|WP_004151744.1|: 1-174 MULTISPECIES: ssDNA-binding

protein [Enterobacteriaceae]

ASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQT

EWHRVVLFGKLAEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGR

QGGGAPAGGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID

NO: 87)

gi|896191520|ref|WP_049200586.1|: 3-176 ssDNA-binding protein

[Klebsiella pneumoniae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 88)

gi|757780461|ref|WP_042999045.1|: 1-175 MULTISPECIES: ssDNA-binding

protein [Gammaproteobacteria]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMK

EQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLG

GRQGGGAPAGGQQQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAAPSNEPPMDFDDDIPF (SEQ ID

NO: 89)

gi|779882983|ref|WP_045362433.1|: 1-173 ssDNA-binding protein

[Enterobacter aerogenes]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEIVVNVGGTMQMLGGRQGGGAPAGGQQQ

GGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 90)

gi|505181644|ref|WP_015368746.1|: 1-174 ssDNA-binding protein

[Enterobacter aerogenes]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEIVVNVGGTMQMLGGRQGGGAPAGGGQQ

QGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 91)

gi|727192951|ref|WP_033654707.1|: 1-177 MULTISPECIES: ssDNA-binding

protein [Serratia]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEQKEKTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGSLQTRKWQDQSGQDRYTTEIVVNVGGTMQMLGGRQGGGAPAGQSAG

GQGGWGQPQQPQGGNQFSGGQQQSRPAQNSAPAASSNEPPMDFDDDIPF (SEQ ID NO: 92)

gi|493870158|ref|WP_006816705.1|: 1-174 ssDNA-binding protein

[Yokenella regensburgei]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEIVVNVGGTMQMLGGRQQGGAPAGGGQQ

QGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 93)

gi|797191136|ref|WP_045853449.1|: 1-178 ssDNA-binding protein

[Raoultella terrigena]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGAEKYTTEIVVNVGGTMQMLGGRQGGGAPAGGGQQ

QGGWGQPQQPQQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 94)

gi|556230820|ref|WP_023284467.1|: 1-174 ssDNA-binding protein

[Klebsiella pneumoniae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQAPSAPSNEPPMDFDDDIPF (SEQ ID NO: 95)

gi|518042119|ref|WP_019212327.1|: 1-181 MULTISPECIES: ssDNA-binding

protein [Yersinia]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEQKEKTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGALQTRKWQDQSGQERYTTEVVVNVGGTMQMLGGRQGGGAPAGGSQ

QDGGAQGGWGQPQQPQGGNQFSGGQTSRPAQSAPAAQPQGGNEPPMDFDDDIPF (SEQ ID NO: 96)

gi|556404007|ref|WP_023305049.1|: 1-174 ssDNA-binding protein

[Klebsiella pneumoniae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVSGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 97)

gi|494949566|ref|WP_007675594.1|: 1-180 ssDNA-binding protein

[Cronobacter condimenti]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANLRLATSESWRDKQTGEMKEVFEWHSVVLYGKL

AEVAGEYLRKGSQIYIEGQLRTRKWQDQSGQDRYSTEVVVNVGGTMQMLGGRQGGGAPAGGNM

GGGQQQGGWGQPQQPQQQSGGAQFSGGAQSRPQQQAPAPSNEPPMDFDDDIPF (SEQ ID NO: 98)

gi|647265972|ref|WP_025713817.1|: 1-174 ssDNA-binding protein

[Klebsiella sp. 10982]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGSQFSGGAQSRPQQQAPAAPSNEPPMDFDDDIPF (SEQ ID NO: 99)

gi|694077789|ref|WP_032423210.1|: 1-174 single-stranded DNA-binding

protein [Klebsiella pneumoniae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANFTLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQDKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQQAPAAPSNETPMDFDDDIPF (SEQ ID NO: 100)

gi|740321204|ref|WP_038158358.1|: 1-176 ssDNA-binding protein

[Trabulsiella guamensis]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQQGAGAPAGGG

QQQQGGWGQPQQPQGGAQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 101)

gi|798873041|ref|WP_045897887.1|: 1-176 ssDNA-binding protein

[Enterobacter cloacae]

MASKGVNKVILVGNLGQDPEVRYLPSGGAVCSVTLATSESWRDKATGELKEQTEWHRIVLFGKLA

EVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQS

QQHGGWGQYQHPQVGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 102)

gi|930179010|ref|WP_054179758.1|: 1-177 ssDNA-binding protein

[Trabulsiella odontotermitis]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQQGAGAPAGGG

QPQQQGGWGQPQQPQGGAQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 103)

gi|908733191|ref|WP_049855775.1|: 1-177 ssDNA-binding protein

[Trabulsiella odontotermitis]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVASEYLRKGSQVYIEGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQQGAGAPAGGG

QQQGGWGQPQQPQQQGGAQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 104)

gi|763074203|ref|WP_043955685.1|: 1-174 ssDNA-binding protein

[Kosakonia radicincitans]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKQTGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGQEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQGGWGQPQQPQGGNQFSGGAQSRPQQSSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 105)

gi|983140349|ref|WP_060448016.1|: 1-177 single-stranded DNA-binding

protein [Serratia marcescens]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEQKEKTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGSLQTRKWTDQAGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGQSA

GGQGGWGQPQQPQGGNQFSGGQQQSRPAQNSAPAASSNEPPMDFDDDIPF (SEQ ID NO: 106)

gi|737931947|ref|WP_035896752.1|: 1-175 MULTISPECIES: ssDNA-binding

protein [Kluyvera]

MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGAEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQ

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 107)

gi|949705074|ref|WP_057057886.1|: 1-175 ssDNA-binding protein

[Enterobacter asburiae]

MASRGVNKVILVGNLGQDPEVRYMPSGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKL

AEVAGEYLRKGSQVYIEGQLRTRKWTDQSGAEKYTTEVVVNVGGTMQMLGGRQGGGTPAGGGQ

QQQGGWGQPQQPQGGNQFSGGAQSRPQQQSAPAPSNEPPMDFDDDIPF (SEQ ID NO: 112)

TABLE 8

SSB Genes Corresponding to the Protein Sequences Referenced by Uniprot IDs.

SSB_ECOLI
I6F672_SHIFL
Q327X2_SHIDS
A0A0A7A3L7_SHIDY

A8AN55_CITK8
I6G8M5_SHIFL
A0A0G3QH78_KLUIN
J1YNM2_9ENTR

A6TGW7_KLEP7
A0A0F6JWW6_ENTAE
L0M840_ENTBF
A0A089UTS5_9ENTR

K8BJX4_9ENTR
A4W5H1_ENT38
A0A0A2VXI7_BEABA
V5CXL1_ENTCL

I2BDV0_SHIBC
A7MPL6_CROS8
D2TRS6_CITRI
Q6D0V9_PECAS

W8TVY4_YEREN
L0MKB5_SERMA
SSB_YERPE
W0L7W3_9ENTR

SSB1_SALTY
A8GK93_SERP5
G7LX00_9ENTR
A0A0K0HGJ5_SALBC

A0A089PKR4_PLUGE
E0SFA5_DICD3
A9MGM8_SALAR
H2IV20_RAHAC

A0A0A1B0L7_9ENTR
A0A0M2KCH9_9ENTR
W0I221_9ENTR
E1SAY5_PANVC

D4GHC5_PANAM
A0A097R6T3_HAFAL
C0ARM9_9ENTR
E6WHP7_PANSA

D8MLY1_ERWBE
A0A0A0Z1Q4_9ENTR
B2VKC4_ERWT9
U3TS91_9ENTR

B4EYJ2_PROMH
Q2NR22_SODGM
D4BZF6_PRORE
D3VBV3_XENNA

J7TBA8_MORMO
K8WA41_9ENTR
Q7MZE3_PHOLL
A0A068QYL1_9ENTR

D3V5U1_XENBS
B7VI95_VIBTL
A0A068R687_9ENTR
I0DSY1_PROSM

A0A0C5W765_9GAMM
C5BF00_EDWI9
A6ARK4_VIBCY
A8T927_9VIBR

Q5E7Z8_VIBF1
SSB_VIBPA
U4K8W8_9VIBR
F0LWT6_VIBFN

F9TAJ8_9VIBR
A0A0F5VDV1_9GAMM
D0Z1S1_PHODD
F7YM47_VIBA7

A0A0A5JJ92_9VIBR
E3BJW7_9VIBR
SSB_VIBCH
F9RB03_VIBSN

A0A097QHM9_9VIBR
Q7MHB8_VIBVY
I2B9U6_SHIBC
A0A0D8MYY3_PHOLE

A0A0D8PTJ3_9GAMM
W0LBZ8_9ENTR
A0A0M2KBM0_9ENTR
A8G8I2_SERP5

A8GJB0_SERP5
Q1ZNU1_PHOAS
A0A068QYS8_9ENTR
C9P2V1_VIBME

A6D019_9VIBR
G2GZS2_9ENTR
A0A068R4G7_9ENTR
E0WRV0_9ENTR

F9T6P1_9VIBR
A0A0M2K592_9ENTR
Q6LM83_PHOPR
G7LLY5_9ENTR

U3U422_9ENTR
F7XX66_MOREP
A0A089PM27_PLUGE
A0A0F5ARE6_9GAMM

J3Z626_9ENTR
A0A090IIF7_9GAMM
D8MJG0_ERWBE
A0A0A7S1F2_9GAMM

R4I1N0_9ENTR
SSB_BPP1
A0A0A1B773_9ENTR
A0A0A1B773_9ENTR

J3TXW1_9ENTR
J3TXW1_9ENTR
SSB2_SALTY
U3U1Y0_9ENTR

D3V012_XENBS
A6AUY7_VIBCY
H2FYH8_OCESG
J1QW29_9ENTR

A0A0F6AES3_9GAMM
A0A0F6AES3_9GAMM
A0KQ40_AERHH
A0KQ40_AERHH

X2H798_9GAMM
A0A0F5ZR91_9GAMM
W8URY6_YEREN
Q1LTY5_BAUCH

A0A0F4PQ78_9GAMM
A1S2X0_SHEAM
C4K6G2_HAMD5
A6TID9_KLEP7

I1E095_9GAMM
I1E095_9GAMM
A0A068QPW4_9ENTR
A6TIR6_KLEP7

A0A0F4QKR0_9GAMM
A0A0E3U3R3_9ENTR
C7R765_KANKD
H0JIY9_9PSED

A0A068QP58_9ENTR
SSB_SHEON
A0A0A7EC64_9GAMM
A0A0A7EC64_9GAMM

I2JLX2_9GAMM
D4G909_RIEPU
SSB_BUCAI
A0A0F4PA03_PSEO7

A8H012_SHEPA
G4QFE0_GLANF
A0A0F6TPU7_9GAMM
W8KKP5_HALHR

SSB_HAEIN
F7WZQ6_9ENTR
A0A0F7M398_9GAMM
Q12IY7_SHEDO

D3V4B9_XENBS
A0A0F6JV15_ENTAE
D3VA12_XENNA
K4KIK2_SIMAS

D3VAC0_XENNA
M4XAW4_PSEDE
D3V9E7_XENNA
C4L8J0_TOLAT

C5BQ98_TERTT
A0A024HBS5_PSEKB
F7NR52_9GAMM
F7NR52_9GAMM

SSB_WIGBR
A0A0M2VIU0_9GAMM
A4A656_9GAMM
E4PMP4_MARAH

A0A0K1QHA3_PSEFL
SSB_PSEPK
C0AX13_9ENTR
C0AX13_9ENTR

F9U8B2_9GAMM
J2VMH4_9BURK
F5ZFB7_ALTSS
F5ZFB7_ALTSS

Q7N7R9_PHOLL
A0A068R6L2_9ENTR
Q3J6T1_NITOC
J3DHJ7_9BURK

C4KC21_THASP
Q21M21_SACD2
Q056Y9_BUCCC
A3N0E5_ACTP2

SSB_PSEAE
I3DML2_HAEPH
A1TYM6_MARHV
W0E0Y1_MARPU

F3KGP2_9GAMM
L0H1Y6_9GAMM
G2DXR4_9GAMM
Q2S941_HAHCH

Q2S941_HAHCH
D3V0M3_XENBS
K0C3K4_CYCSP
D3RQK6_ALLVD

I3YBP2_THIV6
S6BGJ6_9GAMM
J4UTR0_9PAST
D8ISL6_HERSS

A1WEN0_VEREI
K6Z560_9ALTE
Q5QWB9_IDILO
H0PUH7_9RHOO

C3K2U5_PSEFS
W0DPI3_9GAMM
Q0VSH0_ALCBS
M4R5F5_BIBTR

G8PZ64_PSEFL
C1DKP2_ AZOVD
B4EVH3_PROMH
B4EVH3_PROMH

Q15ZK8_PSEA6
Q15ZK8_PSEA6
K0CKB3_ALCDB
SSB_BUCBP

A0A077F648_9PSED
Q4K563_PSEF5
A0A024EEI5_9PSED
K6XV41_9ALTE

A0A0B4XJ04_9GAMM
K6YUU7_9ALTE
B7S2V8_9GAMM
G8M3I5_9BURK

A0A0E3Y7R2_9ENTR
Q487T6_COLP3
Q1YRM7_9GAMM
B8F401_HAEPS

K2IWX5_9GAMM
K2IWX5_9GAMM
W8RQ74_PSEST
H8Z2A9_9GAMM

I4MXA9_9PSED
I0DXT5_PROSM
Q7VQT7_BLOFL
Q2BLP5_NEPCE

F5RHX5_METUF
N6Y596_9RHOO
W0DY92_MARPU
Q603V6_METCA

U7U669_9BURK
A0A090ARY2_9ENTR
Q3IJB1_PSEHT
Q1LRM5_CUPMC

G3IXZ4_9GAMM
G4T2U7_META2
F3KYD2_9GAMM
E5ALT6_BURRH

Q65V18_MANSM
G4MIH5_9BURK
D3RW92_ALLVD
A0A0F7K1E3_9GAMM

SSB_HAEDU
M9X210_MANHA
A0A089YSB8_9PSED
A0A089WKU8_9PSED

D0KVW4_HALNC
D5X2Y7_THIK1
A1WZ36_HALHL
SSB_CUPNH

D7DNA2_METV0
F2G3Q0_ALTMD
A0LDV7_MAGMM
F6AJD7_PSEF1

A4C7X8_9GAMM
E1V5N6_HALED
W0TJZ1_9GAMM
D5AUB1_RHOCB

F9Q6N5_9PAST
Q5P300_AROAE
A0A0F5F0Y4_AVIPA
D4X548_9BURK

H8FXS8_PHAMO
A4XZ57_PSEMY
A0A0D5EH59_9BURK
A1VTY7_POLNA

SSB_COXBU
M1FC50_9ALTE
A0A052IQ42_9BORD
Q0A501_ALKEH

SSB_BORPE
A0A0F2P7X2_9GAMM
W0SH75_9RHOO
W0SH75_9RHOO

V5UG93_9BURK
A9IHN6_BORPD
G0AGI2_COLFT
F4HFM6_GALAU

I8I5Z0_9GAMM
E3HRP1_ACHXA
H3NWG8_9GAMM
A0A089WTH0_9PSED

A0A0H4VYA7_9BORD
U2FYN2_9GAMM
J2TVV7_9BURK
B8GV27_THISH

D5C3D3_NITHN
B8KWJ2_9GAMM
S6ALR8_PSERE
E1VGN3_9GAMM

A4G9L8_HERAR
A0A060B5W2_9GAMM
A0A077LD02_9PSED
B2JCR5_BURP8

W8WTJ8_CASDE
Q145U6_BURXL
C3XAY9_OXAFO
A0A0C4Y4K1_9BURK

D5WBB4_BURSC
Q31IV0_THICR
I0HY81_RUBGI
E1T6K9_BURSG

A0A0F2RU44_9RHOB
F2LSN1_BURGS
B3PK66_CELJU
SSB_PSESM

C6WZ85_METML
A0A0D5V8B5_9BURK
Q122U3_POLSJ
H8GRA6_METAL

I3I6A3_9GAMM
Q5QF93_9CAUD
Q2L1W7_BORA1
G2FAV3_9GAMM

I3UH71_ADVKW
Q166G4_ROSDO
A0A0F5QES3_9RHIZ
G8MQ43_9BURK

A0A0F4QUZ6_9GAMM
A0A0A1H9H4_9BURK
A0A0M2VEY2_9GAMM
A0Z169_9GAMM

I4W834_9GAMM
B1XS47_POLNS
G9ZF80_9GAMM
A0YGK8_9GAMM

SSB_PASMU
Q1GYJ8_METFK
F4GAZ0_ALIDK
G8QL94_DECSP

A4T051_POLSQ
C9R230_AGGAD
I3BZ36_9GAMM
A4JUL0_BURVG

A0A090BUS7_9GAMM
I9KSJ0_9RALS
A0A085BUP3_9RHOB
Q0KEL9_CUPNH

A4VHR0_PSEU5
M4U7M8_9GAMM
M4U7M8_9GAMM
H5WKW5_9BURK

A1TWB0_ACIAC
B7J938_ACIF2
N6YC46_9RHOO
B1XYU6_LEPCP

Q6V7S6_9CAUD
H1G1C8_9GAMM
A0A0F5FLE3_9RHIZ
D0IYB1_COMT2

A0A0F5LKK9_9RHIZ
E1SN23_FERBD
I3CFN7_9GAMM
F4QR90_9CAUL

A0A0F3LFK2_9CAUL
A9BS53_DELAS
A3K1S1_9RHOB
K2C0P8_9BACT

H1S7M6_9BURK
K2JQQ4_9PROT
A0A0F5PUU2_9RHIZ
B8IV04_METNO

Q6F764_ACIAD
C5CYD8_VARPS
A0A0H4L8C9_9RHOB
A4JTB6_BURVG

A1T012_PSYIN
A1KAZ5_AZOSB
D4ZCF2_SHEVD
Q2RTK0_RHORT

K0I6X4_9BURK
A0A070BZW0_BURCE
M4SGM9_9SPHN
E8RTB6_ASTEC

S5XVG2_PARAH
Q5NV32_CUPMC
W0PHB6_9BURK
K2DV93_9BACT

SSB_RHOS4
A0A0B5CJL8_NEIEG
Q0BST4_GRABC
N8TJ62_ACIGI

N8TJ62_ACIGI
C5TD29_ACIDE
D3SCQ7_THISK
Q07WS7_SHEFN

SSB_CAUCR
J5PF93_9RHOB
Q6N604_RHOPA
A0A0F3IJQ8_9GAMM

A2SBY4_METPP
C7RIZ8_ACCPU
E3DH61_9PAST
D0D283_9RHOB

A0A098G838_9GAMM
A0A0F2QS65_9PSED
B0UMW1_METS4
A0A0A1B6N6_9ENTR

Q21RY1_RHOFT
A4JHX0_BURVG
C6RQL8_ACIRA
K2B9K7_9BACT

A1WD45_ACISJ
D3HPN2_LEGLN
T2L5K1_9GAMM
E3I2T1_RHOVT

R4VPZ6_9GAMM
B2UCN7_RALPJ
F4GWZ8_PUSST
J0KHY6_9BURK

G2E4K5_9GAMM
B9NT08_9RHOB
A6VQI4_ACTSZ
A4EQZ7_9RHOB

G2IQI4_9SPHN
A0A0F5LBK0_9RHIZ
K2IPE2_9RHOB
I3DDC4_9PAST

B9JF98_AGRRK
A1AWF7_RUTMC
K1ZXB5_9BACT
A0A0M2WTC2_9BURK

F2LCY9_BURGS
A3V5P2_9RHOB
F0GF15_9BURK
Q0ANG1_MARMM

A0A0D5LSJ4_9RHIZ
SSB_NITEU
A0A0F5FT04_9RHIZ
I1XGC7_METNJ

S6AF57_9PROT
A0A0F4RGQ0_9GAMM
W0UWN0_9BURK
SSB_XANCP

W6K6N2_9PROT
N8ZEX2_9GAMM
N8ZEX2_9GAMM
L0E0L4_THIND

A2SNI7_METPP
B6AXZ3_9RHOB
Q2W4P2_MAGSA
K2Q2L3_9RHIZ

U5T0Q8_9GAMM
A0A076K549_9RHOB
A6FSR7_9RHOB
B1KDD5_SHEWM

J9DXI6_9PROT
A5WGZ1_PSYWF
B9JWR9_AGRVS
R5PPS2_9BURK

I4VXH8_9GAMM
A8LKY4_DINSH
A0A0F2QJX0_9RHOB
D5WNY1_BURSC

A0A0A8K3F9_9RHIZ
S6AQQ7_PSERE
C6XJ14_HIRBI
A4BT12_9GAMM

C4GH79_9NEIS
G9ESV4_9GAMM
B4EAT8_BURCJ
F5SZ57_9GAMM

A0A0A8UPM3_LEGHA
I4YXR6_9RHIZ
G2DYM8_9GAMM
D0CS74_RUELI

A3XAJ4_9RHOB
I1B1H0_9RHOB
A0A098GEA4_TATMI
A0A0F7KCW2_9PROT

Q63XJ3_BURPS
D9QLP4_BRESC
A0A0F3INJ0_9PROT
Q47IU1_DECAR

Q5ZYL6_LEGPH
A3QAA9_SHELP
K2BZG4_9BACT
B1LX07_METRJ

W0TJW4_9GAMM
F4R1K1_BREDI
A4YV28_BRASO
A1VVC7_POLNA

A0A0D6TGJ7_9RHOB
A0A0M2RDE4_9PROT
A1WP86_VEREI
A3UDK5_9RHOB

C6XAV2_METGS
A0A0J6RP75_9RHIZ
A0A0A3VYR5_9GAMM
A5PEQ1_9SPHN

A0A060QI44_9PROT
G2DKU1_9NEIS
W0BBC7_9GAMM
A3TWT6_OCEBH

A0A099GHI8_9RHOB
V2USK2_9GAMM
A0A0F5PFM8_9SPHN
Q0FQ09_PELBH

A4EMD4_9RHOB
Q1GNS9_SPHAL
D0UIT2_AGGAD
A4BEE4_9GAMM

M4NB34_9GAMM
D3SGM4_THISK
A0A023NV45_9GAMM
A0A023NV45_9GAMM

Q1GII7_RUEST
A0A024EMC9_9PSED
A0A0G3LPP7_XANCT
H0HVG1_9RHIZ

K2DU46_9BACT
E1VAE8_HALED
E8TB16_MESCW
A0A0C5KYX1_9SPHN

Q3SSB4_NITWN
A0A0E9MSC8_9SPHN
C5ADI0_BURGB
A0A0M3AV39_9SPHN

Q5NM94_ZYMMO
Q1YHZ0_AURMS
A0A0G1AXI5_9BACT
A0A0F3IMV0_9GAMM

G9ZIW0_9GAMM
Q1QLM1_NITHX
G4CQ97_9NEIS
F5R876_METUF

A3JSR5_9RHOB
B4R9P1_PHEZH
D4YXQ1_SPHJU
G2KQL9_MICAA

E3EYD9_KETVY
R6I5E6_9PROT
B0UTC9_HISS2
Q0BYK0_HYPNA

B7JAE5_ACIF2
A8TKW1_9PROT
D5RPM4_9PROT
J2PJG0_9SPHN

D9SK11_GALCS
Q213Y3_RHOPB
G4E3E4_9GAMM
SSB_RHIME

C5APZ6_METEA
F7QMR1_9BRAD
SSB_RALSO
M9X080_MANHA

B0UVW4_HISS2
F1ZBN2_9SPHN
B3QY18_CHLT3
Q1R0E6_CHRSD

D5CP98_SIDLE
V5SF15_9RHIZ
A0A0D6JBI9_9RHIZ
N9GT97_ACIHA

C0N5M9_9GAMM
A0A0F2RFG2_9RHOB
H6SQ65_RHOPH
G6Y9B4_9RHIZ

R6J5K6_9PROT
F7VE14_9PROT
A0A0F3K8X6_9GAMM
Q1N941_SPHSS

R5R624_9PROT
H0TF15_9BRAD
K5Z2Q0_9PROT
C8NAM6_9GAMM

B0SZT3_CAUSK
C3MD07_RHISN
Q2Y6V2_NITMU
X5MNE3_9RHIZ

I1YK77_METFJ
H8L1R7_FRAAD
B0VN74_ACIBS
SSB_BRADU

G9ZXQ9_9PROT
I3Y923_THIV6
M5DVK8_9GAMM
A3SNZ2_ROSNI

I9L8W8_9SPHN
Q3B682_CHLL7
B7RFT1_9RHOB
G6EYN2_9PROT

G6XK36_9PROT
R5XPX2_9PROT
C7DFD9_9RHOB
A0A077C4P4_9RICK

Q11II2_CHESB
A0A0B5E2T4_9RHOB
E8RPM9_ASTEC
B6JFC9_OLICO

A3VJM3_9RHOB
SSB_RHILO
G3Z3H6_9NEIS
D2ZVB2_NEIMU

A0A0B4X3B3_9RHIZ
D2UDJ2_XANAP
J2WCL8_9SPHN
Q5H2X8_XANOR

J8VZF2_9SPHN
F8J558_HYPSM
B7J8U2_ACIF2
F2JX52_MARM1

F8GEK6_NITSI
D7MZ59_9NEIS
F2BBV5_9NEIS
F9ZVP9_METMM

D5BQS9_PUNMI
Q2G4D9_NOVAD
W5YCT8_KOMXY
E3F1U4_KETVY

V6F1P2_9PROT
A6X111_OCHA4
Q2W772_MAGSA
N0B880_9RHIZ

B9QZI9_LABAD
I4W169_9GAMM
Q5LSX7_RUEPO
F3SAM2_9PROT

A9HM62_GLUDA
W8RVW7_9RHOB
F8GKP0_NITSI
B6ISP2_RHOCS

B2UKJ9_RALPJ
B4S530_PROA2
A7INE3_XANP2
C0DRP8_EIKCO

SSB_BRUA2
Q0FGL9_9RHOB
Q4FR27_PSYA2
Q3SLL6_THIDA

A9D9K8_HOEPD
J1JWK2_9RHIZ
B6JI03_OLICO
F9ZMD5_ACICS

Q2K8E4_RHIEC
B8F4I1_HAEPS
A0A0F2QVS7_9DELT
Q1GG07_RUEST

A7TDK0_NEMVE
I4MIC5_9BURK
W0A7X0_9SPHN
A0A0F5ESE3_AVIPA

A0A0G2ZM37_9DELT
A0A0A8E100_9XANT
A1B8M8_PARDP
V9VTI7_9RHOB

F8GG32_NITSI
Q1DDE2_MYXXD
E6WQZ6_PSEUU
K2FHM2_9BACT

F5T0V4_9GAMM
K2H7N0_9RHOB
W6RH93_9RHIZ
U1XXH6_9BURK

SSB2_XYLFA
A0A0F4RMX3_9RHOB
SSB_AGRFC
A0A0M3ARW7_9SPHN

C1D7M8_LARHH
F9ZLD2_ACICS
U7GLV1_9RHOB
F5Y3Y9_RAMTT

Q5FSE3_GLUOX
A9I2Z8_BORPD
F2A5K5_RHIET
G7URR0_PSEUP

Q0FLP9_PELBH
A0A0F2NW53_9FLAO
A0A0F5EX66_AVIPA
H8L133_FRAAD

B9TDA8_RICCO
Q2NDW0_ERYLH
Q1K3E6_DESAC
M4NHD3_9GAMM

B2UEU3_RALPJ
I5AYV2_9DELT
D5V9T9_MORCR
S5RPZ6_9PROT

SSB_NEIMB
H8YVI5_9GAMM
E5UI11_NEIMU
H0Q1C2_9RHOO

A0A0B4XXZ8_9PROT
A0A0M2LVA1_9SPHN
Q5F7Y0_NEIG1
G8PH47_PSEUV

D5QCC8_KOMHA
G5ZW55_9PROT
A0A0J7KQJ3_LASNI
A0A0F5P8V5_9SPHN

A0A0A6CZ02_9SPHN
S9UWZ3_9TRYP
F6IL26_9SPHN
A0A0F5K5M6_9BURK

Q08U58_STIAD
A5EWP4_DICNV
Q1GGQ8_RUEST
R5Q9P3_9PROT

F9ZU52_ACICS
R6VC65_9BACT
Q28R60_JANSC
H8YW24_9GAMM

C6W2S7_DYAFD
A0A0E9M483_9PROT
M1NYV3_BARAA
G4R9S1_PELHB

L7U4K1_MYXSD
A7HXM1_PARL1
H8MMS9_CORCM
F0F0Z9_9NEIS

A5G056_ACICJ
A0A0G3BHN5_9BURK
E8UF32_TAYEM
G4CGE7_9NEIS

A6W363_MARMS
A0A0M3BUH8_9RHIZ
U5NBX9_9BURK
E0TIG3_PARBH

A0A0D5LKG4_9RHIZ
B3E213_GEOLS
A5EUY7_DICNV
A0A0E3Z3E7_9GAMM

K0PP83_9RHIZ
V9TR38_9PROT
A0A060QDZ1_9PROT
X2HHK0_9NEIS

D5CTA8_SIDLE
A0A097EIF6_9SPHN
M1LT42_9PROT
A0A0G2B146_9BACT

A0A0G2B146_9BACT
I4YX49_9RHIZ
A0A017HB59_9RHOB
A1UST1_BARBK

Q747Z8_GEOSL
R7KPC2_9BURK
A9E9R6_9RHOB
I7EYX3_PHAIB

W0SEU3_9RHOO
A0A0F4QM95_9GAMM
J1K3E2_9RHIZ
M1LV26_9PROT

J1TI96_9RHIZ
Q4FLZ6_PELUB
B2FSL1_STRMK
A0A090N7K3_AFIFE

A6WZ49_OCHA4
A0A0F2R6Z2_9PROT
Q0G045_9RHIZ
Q1IPH6_KORVE

E0MKP9_9RHOB
E8RFQ0_DESPD
D1KBN2_9GAMM
B2IFV1_BEII9

M1NU57_BARAA
A0A0B5FQA0_9DELT
Q39YR9_GEOMG
A0A0C5V225_9XANT

Q7VML3_HAEDU
A0A068T9T5_RHIGA
M9X4F7_MANHA
A0A023Y6B6_9GAMM

B9KI69_ANAMF
A8ID59_AZOC5
H0A2S4_9PROT
M1NS08_BARAA

A5V8E1_SPHWW
A0A0B5FX24_9DELT
Q11LW6_CHESB
C7JGA1_ACEP3

M1PSQ3_DESSD
J7QSP6_METSZ
A7HHA9_ANADF
A0A0F2NLG2_9DELT

A0A075MK72_9RICK
A1BIR8_CHLPD
SSB1_XYLFA
A0A0F5MP96_9RICK

A6FSA2_9RHOB
D0RNY6_9PROT
A0A077AYK7_9RICK
R6Y4F9_9BACT

V4KTJ5_9DELT
R7JQ74_9BACT
M9WYF9_MANHA
Q1NJ37_9DELT

SSB1_CHLTE
L0EU44_LIBCB
E5Y6K0_BILWA
B8ET29_METSB

U6B7F1_9RHIZ
E1QIQ1_DESB2
A0A0A7PM00_9SPHN
D5X2Q2_THIK1

A7BTL5_9GAMM
B8FAA1_DESAA
E4TPK4_MARTH
A5G9S2_GEOUR

Q1N2F3_9GAMM
A8ZRT7_DESOH
A0A0C5W171_9GAMM
A0A0C7DVJ9_9GAMM

C8X289_DESRD
K1PF24_CRAGI
Q2GKN5_ANAPZ
A0A0F5PBJ2_9SPHN

K1YYU4_9BACT
D0LVJ8_HALO1
Q6AK18_DESPS
B9M7M5_GEODF

G6F3F3_9PROT
A0LIN0_SYNFM
H3KDP2_9BURK
I3YP43_ALIFI

B3ENL6_CHLPB
Q2CE60_OCEGH
J9Z216_9PROT
K0NCL2_DESTT

Q2S4M7_SALRD
R5WQX3_9BACT
M9RDW4_9RHOB
Q3A6V5_PELCD

F5S835_9NEIS
A0A0A1H1W5_9BURK
C6XHB3_LIBAP
Q74B90_GEOSL

A0A0C9MSV5_SPHPI
A0A0G3XCA5_9SPHN
Q2S565_SALRD
Q6MMP4_BDEBA

A5V945_SPHWW
A0A0F7KXN7_9SPHN
Q2GG22_EHRCR
F2IZR2_POLGS

A0A0A1H3N2_9BURK
A0A0G3I1G7_LIBAF
R7C2Q7_9BURK
D7AAH9_STAND

B3EB00_GEOLS
D0MG88_RHOM4
A0A0H4VTP9_9BACT
B5EG54_GEOBB

A1AKG6_PELPD
R5EC07_9BURK
C1D7P5_LARHH
A6FSV9_9RHOB

F8AB87_THEID
F8ACN0_THEID
R6EXV2_9BACE
F2IBW4_FLUTR

Q5FHD4_EHRRG
Q11NU5_CYTH3
R5I4N3_9BACT
D6Z5C9_DESAT

J0LLJ2_9BACT
G5H7V8_9BACT
B8DL66_DESVM
A0A077FMS8_9RICK

K7SBZ0_GLUOY
E4U6H4_OCEP5
E4U6H4_OCEP5
A0A099TDX5_9RHOB

F2I194_PELSM
F0JDI6_DESDE
Q2GD12_NEOSM
C4XMH4_DESMR

C1F3G8_ACIC5
A0A0F3NLM9_9RICK
A0A077DE37_9BURK
R6X338_9BACT

A0A0F7JLQ0_9DEIO
A0A0F7JLQ0_9DEIO
A0A0E3UW87_9BACT
H8K3H6_RICAG

X5H4M2_9RICK
A3WGB5_9SPHN
R5ELR6_9GAMM
Q1J216_DEIGD

Q1J216_DEIGD
Q0YQQ4_9CHLB
I7IU14_9RICK
D3PSV4_MEIRD

D3PSV4_MEIRD
R5RTT1_9BACE
A0A0M0BIG3_9ARCH
D7BBU5_MEISD

D7BBU5_MEISD
SSB_RICPR
C6C019_DESAD
E6VS39_DESAO

B6WVX8_9DELT
A9B460_HERA2
G7QAM4_9DELT
B4SFF9_PELPB

A0A0A1H6Y8_9BURK
F8EMG1_RUNSL
R5UZH0_9PORP
B8FNJ8_DESAA

Q3AU24_CHLCH
B0VUJ3_ACIBS
R5NYK0_9PORP
Q2IG69_ANADE

K1JZE8_9BURK
M4V949_9DELT
F7XVZ1_MIDMI
I2EUM7_EMTOG

A0A0G1C0M7_9BACT
A9WH08_CHLAA
Q9PCW1_XYLFA
SSB_BACTN

C1CXK1_DEIDV
C1CXK1_DEIDV
A0A0A7KJ72_9DEIO
A0A0A7KJ72_9DEIO

H8GPM1_METAL
I3TKW7_TISMK
E2CQP0_9RHOB
Q311Q4_DESAG

R5BSB1_9BACE
A0A0G1LQS6_9BACT
W5WYE9_BDEBC
Q9PG20_XYLFA

U6KT59_EIMTE
E1WYM5_HALMS
L0FWX8_ECHVK
I5C772_9BACT

A0A0G1LK57_9BACT
B5JYB4_9GAMM
A0A0G0ET98_9BACT
A0A0C3RCU9_9PORP

U6MFA4_9EIME
F0SN41_RUBBR
E8U544_DEIML
E8U544_DEIML

R5E1D3_9BURK
D6D4Z2_9BACE
A0A0F3MRG0_9RICK
R7IIR5_9BURK

F4HBY7_GALAU
A0A0F2NAQ0_9DELT
H8GSU7_DEIGI
H8GSU7_DEIGI

F9ZRW3_ACICS
I0K538_9BACT
A0A0A7LI72_9BACT
W8F2H2_9BACT

E8V7N3_TERSS
A0A068JL68_9DELT
A0A0F3PJ45_ORITS
G8R207_OWEHD

F2NKT7_MARHT
F2NKT7_MARHT
K1YDW1_9BACT
R4YT41_OLEAN

A0A077DAY8_9BURK
J5MVP4_PASMD
K1Y243_9BACT
A0A0F3QLF9_ORITS

A5CE60_ORITB
A0A0F3MAH9_ORITS
I4C0I0_DESTA
A0A0G1U4Z8_9BACT

F2NJT4_DESAR
W0EUN4_9PORP
D9SEK8_GALCS
F9Z3N4_ODOSD

Q2LVL9_SYNAS
X5DYT2_9BACT
K2E296_9BACT
K2BHI4_9BACT

A0A0F9Z3H3_9BACT
E8RHY9_DESPD
A0A0F2N9J8_9DELT
U6GLJ7_EIMAC

Q5DY59_VIBF1
H8KSH1_SOLCM
R7DJS5_9PORP
A6LDG4_PARD8

A0A078KHX1_9GAMM
A0A0E9LW73_9BACT
R5IPM2_9PORP
A0A0G1GC11_9BACT

W5YLS9_KOMXY
B8J4A9_DESDA
Q729X5_DESVH
L0A2S5_DEIPD

L0A2S5_DEIPD
L3ZDQ8_TERRK
E8RFA5_DESPD
D6SQC2_9DELT

E8UF33_TAYEM
G9ZJG1_9GAMM
C7PL90_CHIPD
I4AKI7_FLELS

R6KSH1_9BACE
T2GG32_DESGI
A7ILF7_XANP2
W0RJ13_9BACT

A0A0G1EUE3_9BACT
C1A4G5_GEMAT
A0A0G1CIP0_9BACT
A0A0E9MWL8_9SPHI

M1WUK7_DESPC
C9RPL0_FIBSS
B3SED6_TRIAD
A0A0G1KPV8_9BACT

R6T4B7_9BACE
F3Z0I0_DESAF
A1AL61_PELPD
B3EUI3_AMOA5

H8H1V2_DEIGI
H8H1V2_DEIGI
F0RJS5_DEIPM
F0RJS5_DEIPM

R4YJJ6_OLEAN
A0A0G0RHW4_9BACT
A6CAL9_9PLAN
F3PPY3_9BACE

C7LVB0_DESBD
U5Q9T7_9BACT
R6DQS0_9BACE
A0A0G0G4A2_9BACT

A5UYQ6_ROSS1
R5U903_9BACE
M9X2N2_MANHA
F0SAP4_PSESL

F8WWX3_9PORP
R6S0F0_9BACE
A0A0G0EYQ7_9BACT
A0A0G1M3J1_9BACT

D2QT14_SPILD
E8WX41_GRATM
A0A0G1B6J4_9BACT
F0RQ97_DEIPM

F0RQ97_DEIPM
A0A0G1WDY0_9BACT
A0A0G1XZ90_9BACT
A0A0G1GD47_9BACT

A0A0G0QXB5_9BACT
F6GHH8_LACS5
Q1MR30_LAWIP
G8P175_GRAMM

A0A0G3Q6R7_KLUIN
A0A0G1NBX6_9BACT
A0A0G1LCA4_9BACT
E8MY37_ANATU

F8C2X7_THEGP
K1Z192_9BACT
A0A0G1PFQ8_9BACT
A0A0G1QNL8_9BACT

R5J536_9BACE
A0A0G1ZBQ4_9BACT
A0A0G1QIF9_9BACT
Q5LF22_BACFN

A0A075WVB3_9BACT
A0A0G0FRM6_9BACT
A0A088EU06_9SPHI
A0A0G1S3A5_9BACT

A0A0G1H5P6_9BACT
A0A0G1WM19_9BACT
A0A0G1S5L4_9BACT
F4CDG5_SPHS2

A0A0M3CBN8_9SPHI
R6LH35_9BACE
K2F210_9BACT
A0A0G0NGV8_9BACT

A0A0G1D0J1_9BACT
A0A0G0R7V0_9BACT
A0A0G0AR01_9BACT
G1UTS6_9DELT

K2GD81_9BACT
A3HUW7_9BACT
A0A0G0ZFU3_9BACT
A0A0G1DJ74_9BACT

D7VHY4_9SPHI
F5J436_9PORP
A0A0G0VD89_9BACT
A0A0G1BMC4_9BACT

I2GP99_9BACT
Q5NE96_FRATT
K2CK60_9BACT
A0A0G0ITC8_9BACT

A0A0G1AFR7_9BACT
A0A0G0P9E8_9BACT
G8TNT5_NIAKG
K2FL67_9BACT

A0A0G1Y159_9BACT
A5URI9_ROSS1
E6SPC5_BACT6
A0A0G0UQH2_9BACT

SSB_THET8
SSB_THET8
A0A0G1EQ71_9BACT
V5RU84_9BACT

K2AY54_9BACT
A0A0M2XR79_9SPHI
I4EDL5_9CHLR
A0A0F9Z444_9BACT

D7CWQ9_TRURR
D7CWQ9_TRURR
A0A0G0AM08_9BACT
W0F027_9SPHI

A0A0F9YYB5_9BACT
R5YWC8_9BACE
A0A0G1TXK3_9BACT
K2ECV4_9BACT

A0A0G0HE85_9BACT
C0BIE9_FLABM
A0A0G1ZAW4_9BACT
E4RV70_LEAB4

E0TIQ6_ZINIC
SSB_DEIRA
SSB_DEIRA
G0IVW4_CYCMS

A0A0G1F613_9BACT
A0A0G0X959_9BACT
A0A0G0AR32_9BACT
G1UNE4_9DELT

F3S3G5_9PROT
R6YPN2_9BACE
E4T8K4_PALPW
A0A0G1GDN2_9BACT

A0A0G0IUI9_9BACT
E5Y6F6_BILWA
A0A0G1VM78_9BACT
R5NF50_9BACT

A0A0G0K483_9BACT
A0A0G1WKB5_9BACT
Q01NH0_SOLUE
A0A0G1IWE9_9BACT

K1KIB0_9BURK
K2DBA5_9BACT
A0A0G0FD70_9BACT
A0A0G1L815_9BACT

A0A0G0TAY3_9BACT
I2K4V5_9PROT
A0A0G1GTA0_9BACT
A0A0G0BES3_9BACT

A9AWI3_HERA2
A0A0G0VFF3_9BACT
A0A0G0Z742_9BACT
T2KR13_9FLAO

A0A0G1RWB7_9BACT
K2DHT4_9BACT
K2FUX9_9BACT
A6EB39_9SPHI

A0A0G0M9E2_9BACT
A0A0G0MVY3_9BACT
A0A0G0QNI7_9BACT
C6XY89_PEDHD

F4B1C6_DOKS4
S2DJ49_9BACT
A0R7W8_PELPD
A0A0G0F0A3_9BACT

I4AL65_FLELS
R6WXR8_9PORP
I3Z3D3_BELBD
R7EGY2_9BACE

K2CX72_9BACT
A0A0G1MUL2_9BACT
A0A0G0WVC5_9BACT
A0A0G0XAK9_9BACT

K2ECW7_9BACT
A0A0G0DA92_9BACT
A0A0G0Z340_9BACT
A6EM42_9BACT

A0A0G1CSF5_9BACT
D1Y258_9BACT
A0A0G1XSH5_9BACT
A0A0A7LLS2_9BACT

E6X251_NITSE
A0A0G0RUZ2_9BACT
A0A0G0KLW8_9BACT
A0A0G0MIW7_9BACT

A0M7C0_GRAFK
A0A0G0L6I2_9BACT
A0A0G0JKX6_9BACT
A0A024FGJ0_9FLAO

A0A0G0MT32_9BACT
A3XQ83_LEEBM
K2AGX2_9BACT
A0A0G1KDM0_9BACT

K1L8G3_9BACT
A0A0G0IL11_9BACT
H6L379_SAPGL
A0A0G0UDL8_9BACT

H1XZM6_9SPHI
F4L5F7_HALH1
C3XLE9_9HELI
F8X2D4_9PORP

S0GEM3_9PORP
A0A0G1XKI1_9BACT
A0A0G1YIP2_9BACT
H1XTH2_9BACT

A0A0G0ZVR8_9BACT
F3ZU02_9BACE
A0A0G1KCC6_9BACT
A0A0G1KMS5_9BACT

A0A0G1PCV8_9BACT
K2CTZ4_9BACT
A0A0G1BVU4_9BACT
A0A0G1QUX1_9BACT

A9FZR5_SORC5
A0A0G1RE30_9BACT
C1A431_GEMAT
A0A0G1RNU5_9BACT

K2AKT1_9BACT
A0A0G1T277_9BACT
K1ZC65_9BACT
X5DJQ1_9BACT

A0A0G0Q4T9_9BACT
A0A0G1EN32_9BACT
A0A0G1HYN1_9BACT
B4CYA0_9BACT

A0A0G0H3K7_9BACT
A0A0G0JR80_9BACT
I3C4X1_9FLAO
A0A0G0ICH2_9BACT

A0A0G0Q0Z9_9BACT
Q26HH7_FLABB
F8X1E8_9PORP
K1Z5D0_9BACT

A0A0G1PK08_9BACT
A0A0G0QT25_9BACT
A0A0G0VCD2_9BACT
A0A0G0WJ75_9BACT

A0A0G0QRF0_9BACT
A0A0G0XSM8_9BACT
A0A0G1IVQ6_9BACT
A0A0G1U187_9BACT

A0A0G1FGT8_9BACT
A0A0F9YZ74_9BACT
A0A0G1KD14_9BACT
K2GCJ2_9BACT

F9TDK3_9VIBR
K2AA51_9BACT
A0A0G1M0Y6_9BACT
K1ZD39_9BACT

A0A0G4B559_9BACT
A0A0G1QXE6_9BACT
A0A0G1VT24_9BACT
A0A0G0K489_9BACT

A0A098BVY9_9PORP
K2AQC8_9BACT
A0A0G1MW79_9BACT
A0A0G1V0T6_9BACT

R7PAL0_9BACT
K2G2N2_9BACT
A0A0G0BK79_9BACT
A0A0G0GUJ0_9BACT

F8EP30_RUNSL
A0A0G0HDB0_9BACT
A0A0G0M3V5_9BACT
A0A0G1HM27_9BACT

A0A0G0T5R8_9BACT
A0A0G1CE07_9BACT
A6G1X8_9DELT
A0A0G0YX12_9BACT

A0A0G0UM02_9BACT
A0A0G1YNF5_9BACT
E6X5Q0_CELAD
A0A0G1WNB0_9BACT

A0A0G0YK69_9BACT
A0A0G1VSA9_9BACT
A0A0G0M6X1_9BACT
A0A0G1P2X8_9BACT

X2H7A7_9NEIS
A3U5V5_CROAH
A0A0G1I503_9BACT
K2GR66_9BACT

SSB_RHOBA
A0A0G1TA05_9BACT
R5JRY1_9BACE
A7GXQ3_CAMC5

A0A0G1TVB4_9BACT
A0A0G1R3J3_9BACT
F0R206_BACSH
A0A0G1I1J8_9BACT

A0A0G1N1Y0_9BACT
A0A0G0DFF8_9BACT
A0A0G0HJ81_9BACT
A0A0G0L6C8_9BACT

G8UNV5_TANFA
A0A0G0WNN8_9BACT
A0A0G0T067_9BACT
A0A0G1TTV3_9BACT

R6FJU2_9BACE
K2Q6V2_9FLAO
G9PZM7_9BACT
A0A0G1EVI1_9BACT

K2CX80_9BACT
D2ZSZ5_NEIMU
A0A0G0MJX6_9BACT
A0A0G1UL86_9BACT

A0A0G1T611_9BACT
A0A0G1NY98_9BACT
A0A0G1K096_9BACT
A0A0G1LVX9_9BACT

A0A0G2AUH0_9BACT
A0A0F9ZKM8_9BACT
A0A0G0GFH0_9BACT
A0A0G0D4N4_9BACT

A0A0G1KT99_9BACT
R7LF57_9BACT
G2EHB5_9FLAO
A0A0F9YE88_9BACT

A0A0G1LER9_9BACT
A0A0G1E5Q5_9BACT
K2DE88_9BACT
A0A0G0QWW2_9BACT

A0A0G1YHP1_9BACT
Q30PZ3_SULDN
A0A0G1YJ06_9BACT
A0A0G0RMN5_9BACT

H2BRZ7_9FLAO
D5SQI8_PLAL2
A0A0G0RM55_9BACT
D1B1C3_SULD5

A0A0G0IK26_9BACT
A0A0G0RPA2_9BACT
K2ES05_9BACT
A0A0D5YSC5_9FLAO

A0A0G0FI04_9BACT
A0A0G0JPG5_9BACT
A4V7V9_PSEFS
L7W8Z0_NONDD

A0A0G0ZNV6_9BACT
K2B5H2_9BACT
A0A0G1GWI5_9BACT
A0A0G0VHY0_9BACT

A0A0G0F0G2_9BACT
W2CHQ2_9PORP
A0A0G1C1H8_9BACT
A0A0G1VPU9_9BACT

A0A0G1Y128_9BACT
A0A0C5W6B5_9FLAO
W5YUV6_9ALTE
A0A0G0QW90_9BACT

F0P2N0_WEEVC
A0A0G1WRU3_9BACT
V6F187_9PROT
V6F187_9PROT

G0L3S2_ZOBGA
A0A0G1NZT4_9BACT
B3JPX7_9BACE
A0A0A8E408_9GAMM

A0A0G0F4Z5_9BACT
K2CZW8_9BACT
R6EFS7_9BACE
R7JPX4_9PORP

A0A0G0B811_9BACT
R6NQA9_9BACE
A0A0G0HF46_9BACT
A0A0G0TF81_9BACT

A0A0G1DZP7_9BACT
K2BD14_9BACT
A0A0G2BH32_9BACT
R6CU93_9BACE

H0ULU9_9BACT
A0A0G1R5Q2_9BACT
D2Z2X8_9BACT
C9LG13_9BACT

A0A0G1G841_9BACT
A0A0G1DYE2_9BACT
A0A0G1MXS6_9BACT
A9WIF6_CHLAA

V6F590_9PROT
V6F590_9PROT
A0A0G1KC57_9BACT
A0A0G0ZZ46_9BACT

A0A0G1KHX6_9BACT
A0A0G1LKX5_9BACT
A0A0G0Y5Y6_9BACT
R6ZUD7_9BACE

E4U134_SULKY
D8K0D1_DEHLB
A0A0G1E8Q7_9BACT
A0A0G0WUP2_9BACT

A0A0G1RR84_9BACT
C0ZA46_BREBN
L0EUZ9_LIBCB
A0A0G0UEZ2_9BACT

A9G766_SORC5
A0A074SUT6_HAMHA
A0A0G0HVX2_9BACT
A0A0G0JF99_9BACT

I3UHZ1_ADVKW
A0A0F9Z7C7_9BACT
J2HR04_9BACL
K4IS14_PSYTT

D5ERI0_PRER2
A0A0G1U855_9BACT
A0A0G1W834_9BACT
A3U5F1_CROAH

F9YQ81_CAPCC
B9L5W8_NAUPA
D5EJK6_CORAD
A0A0G0NQR7_9BACT

A6L7N8_BACV8
G2LEN2_CHLTF
A0A0G1LDB3_9BACT
A0A0G0WK72_9BACT

A0A0G1XPK7_9BACT
R7NMI1_9BACE
A0A0G1UAR8_9BACT
R7HTQ0_9BACT

I3YV25_AEQSU
C0BLH8_9BACT
K1ZZU0_9BACT
F0EYB2_9NEIS

A0A0G0LTD2_9BACT
F6GGX7_LACS5
K1Z9M5_9BACT
A0A0G1R9V2_9BACT

H1Y6M1_9SPHI
K1ZMT3_9BACT
A0A0G0IWK6_9BACT
A0A0G0JF01_9BACT

A0A0G0GJN5_9BACT
A0A0G0L264_9BACT
A4BXL3_9FLAO
F4KZC1_HALH1

A0A0G0P2K6_9BACT
A6Q7C8_SULNB
A0A0G0WB84_9BACT
A0A0G1YS56_9BACT

A0A0F0CQF3_9BACT
Q4XMV9_PLACH
A0A0G1NYY3_9BACT
B6YQX2_AZOPC

A0A0G1BF33_9BACT
A4AWE0_MARSH
A0A0G1NN17_9BACT
D6THA2_9CHLR

SSBA_BACSU
L8AVZ9_BACIU
A0A0G1T421_9BACT
A0A0G0U7M6_9BACT

SSB_WOLSU
R5BM18_9FIRM
A0A0G1C6D3_9BACT
A0A0G2AWX5_9BACT

I4A267_ORNRL
R5GFV4_9BACT
K6UKM8_9APIC
D1BR58_VEIPT

J2S2V5_9FLAO
A0A0G1HLF4_9BACT
A7ZEF7_CAMC1
H7FN74_9FLAO

A0A098GHM2_TATMI
A0A0E9MZ46_9SPHI
W7AIE7_PLAVN
A8ZR98_DEIGD

A8ZR98_DEIGD
I0IET8_PHYMF
B9L1I8_THERP
A0A0G0M358_9BACT

A0A0G0RPU1_9BACT
V6S8T9_9FLAO
E0UPU6_SULAO
A0A0G1BTT0_9BACT

A0A0G1FMR0_9BACT
A0A0G0QLF7_9BACT
A0A0G0BZ87_9BACT
A0A0G1FFL8_9BACT

A0A0G0CXZ0_9BACT
A0A0F9ZUR4_9BACT
A0A0G1FVR4_9BACT
A0A0C1GCK9_9FLAO

A0A0G0MGM9_9BACT
A0A0G1WN93_9BACT
A6LAC3_PARD8
G2PM60_MURRD

A0A0G0GC72_9BACT
B6BIM9_SULGG
A0A0G1WQA3_9BACT
D7JFM1_9BACT

C5ZV90_9HELI
A0A0G1QS53_9BACT
L7W5D1_NONDD
G2Z2B5_FLABF

Q8I415_PLAF7
A0A024VHA1_PLAFA
A0A024WBS3_PLAFA
A0A024WWR3_PLAFA

D1B9D4_THEAS
A0A0G0BWT4_9BACT
A2TXU5_9FLAO
A0A0G1AL26_9BACT

A0A0G0UT33_9BACT
A0A0G1XX97_9BACT
A9DL76_9FLAO
A0A0G0L6C1_9BACT

A0A0G0IFR9_9BACT
Q7PDM6_PLAYO
A0A0G1NEE6_9BACT
A0A0G1RE35_9BACT

A0A0G0IKV8_9BACT
G0LBI4_ZOBGA
A0A0G0Q0U8_9BACT
AIZRR4_9BACT

A0A0G0K4P7_9BACT
C8PLH8_9PROT
W8VWG1_9FLAO
A0A0G0C6A8_9BACT

A8ERE3_ARCB4
A0A0G1FD97_9BACT
A0A095ZH18_9BACT
A3J2K3_9FLAO

Q4Z5Q5_PLABA
A0A0G1IAL7_9BACT
I0WK08_9FLAO
A5FLM3_FLAJ1

A0A0F9ZAH0_9BACT
D1PYR4_9BACT
A4CIT5_ROBBH
A0A0G0G6E6_9BACT

W7A536_9APIC
R5AVX0_9BACE
A0A0F2NZ96_9FLAO
A0A0G1PPZ1_9BACT

G2Z404_FLABF
A0A0G1P7K3_9BACT
A0A060R893_9BACT
A0A0G1N320_9BACT

W8VVV0_9FLAO
K2A8G9_9BACT
K2E6G7_9BACT
A0A0G0RN27_9BACT

A0A0G1H3J6_9BACT
A5KB16_PLAVS
K0P2K0_9BACT
D5V760_ARCNC

A0A0G0YX77_9BACT
A0A0G0MWK9_9BACT
Q3Z7N8_DEHM1
A0A024FGV6_9FLAO

Q5ZWV8_LEGPH
A0A0G1W343_9BACT
A0A0G0E4G6_9BACT
W0J168_9BACT

D1CFJ0_THET1
A0A0G0VDL7_9BACT
S6A3X9_9SPIO
A0A0G1N6K0_9BACT

E6TZX5_BACCJ
B3L6I1_PLAKH
F0IH13_9FLAO
E3CX16_9BACT

L0D9A4_SINAD
A0A0G1QE90_9BACT
A0A0G1ZNC1_9BACT
D5EG56_AMICL

E6K7H1_9BACT
A0A0G1ZLY4_9BACT
T1JEX3_STRMM
T1JEX3_STRMM

A0A094WDC8_BACAO
SSB_HELHP
G8X6H6_FLACA
H1Y6X3_9SPHI

A0A0B5RL43_9FLAO
A0A0G0R2B1_9BACT
G8R7E5_OWEHD
T2KIN2_9FLAO

C2M318_CAPGI
R7D092_9BACE
A0A0C5W9G8_9FLAO
Q7MXD2_PORGI

A0A0G1L2P3_9BACT
K6D849_BACAZ
W0F1G0_9SPHI
A6H058_FLAPJ

G2PIF9_MURRD
A0A0G0VWD9_9BACT
A0A0G0MTU3_9BACT
I3C2X3_9FLAO

C3J7T3_POREA
D5B9K6_ZUNPS
F0RQ90_DEIPM
F0RQ90_DEIPM

X2GU58_9BACI
A0A089NRR5_9BACL
F4B204_DOKS4
A0A0G0J2C4_9BACT

Q65CP4_BACLD
A4CH56_ROBBH
A0A0G1CG12_9BACT
A0A0M2V033_9BACT

A0A0G1X8Y6_9BACT
A0A0G1W4C5_9BACT
K2AP50_9BACT
R5AJM2_9BACT

Q26FF9_FLABB
F0RE93_CELLC
A0A0G1DJJ4_9BACT
J3C846_9FLAO

A6Q465_NITSB
I3C4Z1_9FLAO
A0A0G0XVB6_9BACT
A0A0G0GYK1_9BACT

H0E9H1_9ACTN
I4BUS1_ANAMD
A0A0G0UV05_9BACT
D1VYB4_9BACT

C8PFI3_9PROT
F4XGC9_9FIRM
K2F5S8_9BACT
A0A0G0XSG0_9BACT

SSB_BACHD
A0A0F6SHL5_9DELT
A5FJQ5_FLAJ1
A0A0G0CPI5_9BACT

V4IHX5_9GAMM
C6RCT1_9PROT
C6D836_PAESJ
B9KCI7_CAMLR

H8XRQ3_FLAIG
H8XQA3_FLAIG
A0A0G1N5F5_9BACT
G2PPT0_MURRD

A0A0G0RBE5_9BACT
A0A0G1LMR6_9BACT
A7I007_CAMHC
A3J3H8_9FLAO

A0A098GC49_TATMI
F0RQ78_DEIPM
F0RQ78_DEIPM
A1U8M6_MARHV

R5ZXU7_9BACT
A0A0G1TFJ3_9BACT
A0A0G1D250_9BACT
A0A0C1G1B6_9FLAO

K2D8C8_9BACT
G2ED13_9FLAO
A8FJE6_BACP2
D3FQF9_BACPE

V4Z7I0_TOXGO
H1ZEI1_MYROD
A0A0G0AIB5_9BACT
V6SCA1_9FLAO

A4ANB7_MARSH
A0A0B4SE50_9BACI
A0A0G0XMP0_9BACT
I7HEB8_9HELI

A0A0G1MV34_9BACT
H2BXS6_9FLAO
H3SKZ8_9BACL
A6EQD6_9BACT

I0JTK6_HALH3
A0A0G0KMQ6_9BACT
J9QTR6_RIEAN
A0A0G0X1K5_9BACT

E6X1S0_NITSE
A0A089MFZ3_9BACL
A0A059NVS1_9BACI
D8DWS8_PREBR

A0A0K0G707_9FIRM
K2C4X2_9BACT
I3C1I5_9FLAO
A0A0G1QFF4_9BACT

G2PQ85_MURRD
A0A0G0R1X3_9BACT
H8H312_DEIGI
H8H312_DEIGI

B7GMU8_ANOFW
K4RIT0_HELHE
SSB_CAMJE
G9ERB0_9GAMM

R5BTT7_9FIRM
I3C0K2_9FLAO
A3J3H9_9FLAO
A0A0G1A645_9BACT

A0A0G0EG92_9BACT
K1XT39_9BACT
A0A0G0NJ04_9BACT
A0A0G0E1X4_9BACT

A0A0G0DA43_9BACT
A0A0G1R810_9BACT
A0A0G1KQT7_9BACT
A0A0G1KYP1_9BACT

C2WF66_BACCE
G9ZIP3_9GAMM
A3XH90_LEEBM
I3C290_9FLAO

K2F3J1_9BACT
A6L491_BACV8
I3C2L6_9FLAO
W8VX23_9FLAO

I0TC03_9BACT
B4RIS2_PHEZH
G9EJ05_9GAMM
K1YY81_9BACT

A0A0G1G5A2_9BACT
A0A0G0YPJ7_9BACT
D8PBE8_9BACT
H2BTI1_9FLAO

H7F723_9LIST
SSB_BACCR
R6Q4G8_9BACT
D5BB32_ZUNPS

C0QIM1_DESAH
I3CB97_9FLAO
K1JX68_9BURK
A0A0G0AW48_9BACT

I3II60_9BACT
G9YQV9_9FIRM
R6E6X3_9BACT
K9P7I4_CYAGP

R6EX32_9BACT
A0A0F7CTW4_9CHLR
H8XRQ4_FLAIG
R5PDP4_9BACT

B9D1K8_CAMRE
R6YS16_9BACT
B5IJJ3_9CHRO
SSB_BACAN

D9RRS8_PREMB
D3UJ32_HELM1
K2GWT5_9BACT
F2KTM6_PREDF

G8T6V2_NIAKG
A3XJ25_LEEBM
A0A0M2PGH3_9BACI
E0I9A3_9BACL

G9EQ89_9GAMM
C7MM82_CRYCD
E0NPE2_9BACT
D7NFG4_9BACT

C7M3U9_CAPOD
G3Z1H6_9NEIS
Q5KU70_GEOKA
E6MT69_9BACT

G7V667_THELD
R7F3A0_9BACT
R6CIQ6_9BACT
E4PJ71_MARAH

D6D7Q4_9BACE
K2F7X5_9BACT
F2LU75_HIPMA
A0A0G1TBB5_9BACT

A0A0G3EDN0_9BACT
D5BAB2_ZUNPS
B9XFD3_PEDPL
A2TY45_9FLAO

C9LYI1_SELS3
A0A089M198_9BACL
R6AVL5_9BACT
A0M128_GRAFK

A0A075RC04_BRELA
E7RSX3_9BACT
R5VBW7_9BACE
I3C8E4_9FLAO

A7GVN6_BACCN
A5GI32_SYNPW
I3C6Z7_9FLAO
R7RV76_FLAPJ

R5TDE7_9FIRM
G8X8S0_FLACA
A0A099UZI7_9HELI
A0M0Q6_GRAFK

I0WHP7_9FLAO
F8N6Q2_9BACT
A0A075LKI3_9BACI
A0A099WCC6_9LIST

A3Z466_9SYNE
R6WUN6_9BACT
A0A0C2UAE7_BACBA
C5CD95_KOSOT

H2BWP6_9FLAO
B3E0Q5_METI4
A0A0F5PBD5_9SPHN
I8J2Y5_9BACI

R6WM24_9BACT
A0A0F5I2L0_9BACI
F4GLF7_SPHCD
A0A0G1YCE6_9BACT

SSB_OCEIH
A0A0G1JU97_9BACT
K2CKU7_9BACT
K6Y9X8_9ALTE

I3YUH0_AEQSU
A0A0G0R4I9_9BACT
A0A088AIU7_APIME
J7J598_DESMD

K2QGZ2_9FLAO
L0G1L9_ECHVK
A9DQ41_9FLAO
T2KN55_9FLAO

I3C1Q8_9FLAO
E3EC34_PAEPS
I1YFJ8_METFJ
A0RQ07_CAMFF

G7WI54_DESOD
A0A0D5NIC7_9BACL
A0A0F2SDU7_9FIRM
D5BLX7_ZUNPS

U5L4I2_9BACI
A0A0G1NI47_9BACT
D2R6N7_PIRSD
A0A0G1GT67_9BACT

B4EWR6_PROMH
X4ZJY3_9BACL
R5KHV0_9BACT
D3LTM2_9FIRM

B9D160_CAMRE
E1KTQ3_9BACT
D5BB33_ZUNPS
I3C289_9FLAO

A0A077KJT9_9FLAO
Q05UI4_9SYNE
A0A0B5AYD2_9BACL
A3ZL43_9PLAN

A3YWU7_9SYNE
F0RBC8_CELLC
D3IHP9_9BACT
J2ZK91_9LACO

B9DIB9_STACT
A0A0G1DV39_9BACT
K1ZYT4_9BACT
A0A0E3UIY2_9BACT

E8N1Q1_ANATU
L0ELE3_THECK
L8HBV6_ACACA
K2FTU8_9BACT

A0A075R7Q5_BRELA
K0J855_AMPXN
J5KD76_PASMD
A0A0D1LP27_9LACT

I0I2P5_CALAS
D4KHG9_9FIRM
F2F271_SOLSS
C6IWW3_9BACL

A0A0G1EHZ2_9BACT
B2UN66_AKKM8
I0IMR3_LEPFC
J3CHS7_9FLAO

A6M3L1_CLOB8
R7EVC4_9FIRM
G2KTA2_LACSM
Q5M2Q4_STRT2

R5HF76_9FIRM
A0M601_GRAFK
Q0IDX5_SYNS3
E6X7W5_CELAD

Q11BM6_CHESB
H6NIY5_9BACL
A0A0G0V6P2_9BACT
R4PN35_9BACT

L0JB55_PREDD
A0A0G1B4T5_9BACT
A0A0A1GUA2_9LACO
G4RDQ8_PELHB

E3GR78_EUBLK
E3GR78_EUBLK
A5GQ52_SYNR3
L3TXZ4_ENTFC

F3ZYJ8_MAHA5
G4HDI8_9BACL
H1HQJ5_9BACT
R6G607_9CLOT

J9ZDX0_LEPFM
R7KS27_9FIRM
D4MAR0_9BACT
B5YK70_THEYD

G2TIH3_BACCO
A5CY51_PELTS
A3I0E4_9BACT
Q03I53_PEDPA

H5VBY2_HELBI
F2LV66_HIPMA
G2FTW8_9FIRM
D6Y1K5_BACIE

C4L009_EXISA
R7GGC3_9CLOT
A0A0F3RSN3_9LACO
S1P033_9ENTE

Q5WAH4_BACSK
Q4L341_STAHJ
W0JD18_DESAE
K1LGI4_9BACT

Q73M33_TREDE
R2T735_9ENTE
K1XET1_9BACT
T2KQ25_9FLAO

F8KQW0_HELBC
A0A0F2J7A3_9FIRM
A0A0F3RVR0_9LACO
S0L007_9ENTE

G2FNN2_9FIRM
V4IXS5_9GAMM
B1YG98_EXIS2
B2V6V6_SULSY

I7A7D4_MELRP
F0P2C4_WEEVC
A0A0G1IEC5_9BACT
T2KP72_9FLAO

F5LHP3_9BACL
I0AJV0_IGNAJ
R5B767_9CLOT
R7CMF9_9FIRM

R5A556_9CLOT
G1WB69_9BACT
R6FRH6_9CLOT
F4KP50_PORAD

E7NW85_TREPH
A7H0W9_CAMC5
R7KDB0_9CLOT
I4Z6P8_9BACT

K0FER8_9NOCA
V5X6S5_MYCNE
G6EMD8_STRTR
A4BWV3_9FLAO

F2BXW4_9FIRM
I4EL78_9CHLR
E8R1N7_ISOPI
A0A0G3V8F0_9ACTN

A0A077ELF4_9FLAO
B1ZPW4_OPITP
R6W1H8_9FIRM
D5XDR1_THEPJ

L0EKJ7_THECK
A8L8T3_FRASN
H0DJQ8_9STAP
A0A0H4L2E2_9BACI

Q5HRZ4_STAEQ
K2BME1_9BACT
Q7U9Y1_SYNPX
A0A0H5Q455_SYNPZ

A0A0F9Z426_9BACT
G0VMW5_MEGEL
I4DCJ9_DESAJ
C4IMS9_CLOBU

A0A0M2SW57_9BACI
Q24MB8_DESHY
A0A024Q6M4_9BACI
R7MDV2_9CLOT

A0A0G0JLJ7_9BACT
A0A0A2E6F1_9PORP
SSB_THEMA
R7IZP9_9BACT

K6DNP2_9BACI
A0A0F2S1Q7_9RHOB
SSB1_STRA5
A0A077J7B4_9BACI

C2CT55_CORST
F8B6R8_FRADG
Q2G112_STAA8
C6X107_FLAB3

I0RDU7_MYCPH
A0A060M243_9BACI
W1SCF8_9BACI
A0A0A2U562_9BACL

E4KZJ9_9FIRM
K8E172_CARML
Q7NHP8_GLOVI
E8SHU3_STAPH

A4VXG0_STRSY
E3C6V5_9LACO
C1DTM9_SULAA
B0P5X9_9FIRM

A0A0G0B0I9_9BACT
SSB2_LACLA
A0A075JU30_9BACI
K2BLN7_9BACT

K4L900_9FIRM
L0FCF1_DESDL
D5WXD0_KYRT2
H6LCF9_ACEWD

S1N2V3_9ENTE
R5C9B9_9BACT
C4IHH5_CLOBU
A0A023CJV1_GEOSE

D3EJA6_GEOS4
A0A075TYJ5_9LACT
A0A0G0YSF5_9BACT
I4F5M2_MODMB

A0A0G1VJD5_9BACT
R5Z2P9_9CLOT
A9WVL4_RENSM
B9E8Y6_MACCJ

F0T2X6_SYNGF
E6W1E1_DESIS
H2J4V1_MARPK
R5KVV6_9CLOT

M1MNX6_9CLOT
R2PID4_9ENTE
Q7V923_PROMM
I0RXI5_MYCXE

Q0F0E0_9PROT
G9YN40_9FIRM
Q5YN16_NOCFA
R6AK08_9FIRM

G8LTQ4_CLOCD
S0JGT9_9ENTE
I7JHF2_9LACT
F5YAZ8_TREAZ

Q24VF6_DESHY
T0TSW0_9STRE
C2EU98_9LACO
R7MCD0_9CLOT

R5RR34_9FIRM
R5LR09_9SPIR
R7IS09_9CLOT
R6SXG9_9CLOT

K4ZHE9_PAEAL
G8RLC3_MYCRN
Q5LCZ3_BACFN
H7FPY3_9FLAO

R7PT35_9FIRM
D6GT00_FILAD
D4Z2M0_SPHJU
A9BD67_PROM4

A0A0B5RTH7_9FLAO
W0EFP3_9FIRM
A6NVZ6_9FIRM
D3G1D7_BACPE

A0A0F9Z6S5_9BACT
A0A023CI44_GEOSE
H2CE09_9LEPT
C5D8F8_GEOSW

V4QQS1_STRIN
I1YV15_PREI7
S0SK23_9ENTE
D9SPC1_CLOC7

K5BDX9_9MYCO
F4C309_SPHS2
I4DCP3_DESAJ
C0ZV44_RHOE4

G8PE86_PEDCP
D1C3R8_SPHTD
N0B1V2_9BACI
A1UP85_MYCSK

C5RAY6_WEIPA
C5D9X5_GEOSW
U5QLX6_9CYAN
C7NGT6_KYTSD

Q49UQ1_STAS1
A0A0M2VUR8_9BACL
Q38ZR7_LACSS
B9CLT9_9ACTN

B0VFZ2_CLOAI
F4F596_VERMA
A3I0F7_9BACT
I0GSV3_SELRL

I1D897_9PSEU
A0A0G2ZH51_9BACT
D6ZB10_SEGRD
D5UP28_TSUPD

A0A0G0FVV6_9BACT
A0A0A8JFR8_BACSX
A0A0G2B6Q3_9BACT
E1R296_SPISS

A0A0B6TWN0_9CORY
V5WHR9_9SPIO
R6TSJ9_9FIRM
F8B065_FRADG

C0QQW8_PERMH
F9VMA2_ARTSS
R6PML2_9CLOT
A7HZQ9_CAMHC

SSB_MYCS2
SSB_STRP1
R5W1M5_9BACE
K1X5S5_9BACT

SSB1_LISMO
SSB_ENTFA
Q67J49_SYMTH
C9KMR8_9FIRM

M1MYD5_9CLOT
D7CKA0_SYNLT
R6YHT0_9CLOT
R5YGH9_9CLOT

A8F5G1_PSELT
F4LN90_TREBD
A9WB21_CHLAA
A1SQS3_NOCSJ

G5JZW3_9STRE
Q1QEX4_NITHX
D5BDH4_ZUNPS
G7MBJ2_9CLOT

T0U9E3_9ENTE
E5WH96_9BACI
Q0ATX7_SYNWW
H6R9N9_NOCCG

R6UNI3_9FIRM
E1R0L2_9ACTN
F0H2E9_9FIRM
K6TY13_9CLOT

B9DVK3_STRU0
G5K7U2_9STRE
R3X593_9ENTE
R7FCY1_9CLOT

F9HJG1_9STRE
G1WKH2_9ACTN
V9WD61_9BACL
E2ZB85_9FIRM

S5QZ71_9STRE
J9SSA7_9ACTN
H1D1U0_9FIRM
SSB_HELPY

R7L899_9CLOT
A0A0D3VG85_9BACL
U5QBR9_9CYAN
R6H9V2_9FIRM

R6MT98_9FIRM
L0J4W0_MYCSM
A0A090MGC5_AFIFE
R6SVH2_9CLOT

U5N134_CLOSA
C3PKC9_CORA7
SSB_STRMU
A0A0F5N0E8_9MYCO

Q03UD7_LACBA
U5WR80_MYCKA
Q5FN08_LACAC
R6M3F2_9FIRM

R5NQF3_9CLOT
A0A0F2J3B9_9BACT
D0L8B0_GORB4
E8MYY2_ANATU

F9DV30_9BACL
A0A0H4LAQ5_9LACO
A0A0G1W755_9BACT
A0A0H4P943_9BACI

A0A0G3ITE4_9MYCO
E8UYL8_TERSS
A0A0G1HV64_9BACT
I6SBF0_ENTHA

G0J0R1_CYCMS
K9PZ35_9CYAN
K6VEM2_9MICO
D4IXN6_BUTFI

Q5KV10_GEOKA
M1V543_CYAME
I3EBY4_BACMT
R5VRL4_9FIRM

C6HX91_9BACT
A8AZD6_STRGC
B2HIB8_MYCMM
Q3AG26_CARHZ

G5F284_9ACTN
J4WCB5_9FIRM
A0A0B5E162_9RHOB
K2IBX4_9RHOB

A3VKS9_9RHOB
I3Z9F8_BELBD
K4LED7_THEPS
D7GS93_9FIRM

B2GJD7_KOCRD
A0A0A1H2Y5_9BURK
H3NDL1_9LACT
F5YR52_TREPZ

A9BH63_PETMO
C2BHH0_9FIRM
A0A0M3CEV4_9SPHI
S6CKB8_9ACTN

C8W8M1_ATOPD
H6LDD9_ACEWD
Q7NWU0_CHRVO
SSB_CLOPE

Q0SB52_RHOJR
A0A0D6DVT4_9LACT
D3Q1K1_STANL
R5M0T5_9MOLU

C5C7V0_MICLC
A0A0A6D3P2_9SPHN
C6HTW2_9BACT
G0GBN6_SPITZ

Q04HQ6_OENOB
F6CKP8_DESK7
G7CIJ8_MYCTH
F6FWN5_ISOV2

D5MK04_9BACT
A0A0A1FWC9_9MYCO
A6DQM0_9BACT
K9V7L7_9CYAN

R6T680_9FIRM
H9UJF3_SPIAZ
F9VGA9_LACGL
R7F241_9BACI

A0A0F2PHJ2_9FIRM
R6SGE0_9CLOT
D2S814_GEOOG
C0W4X0_9ACTO

B0TA59_HELMI
S1RRM8_9ENTE
E6MIL0_9FIRM
F7YUV7_9THEM

F6B6R2_DESCC
F3ZWZ1_MAHA5
F2NYC3_TRES6
F2IIU9_FLUTR

A0A0M2HUHL_9MICO
F7NE40_9FIRM
A0A060QK02_9PROT
A0A0M2SZ93_9BACI

A0A099WPE6_9LIST
R4KWF9_9FIRM
R6XT61_9CLOT
C7MS26_SACVD

I7L720_9LACO
J6IJK1_9ACTN
A0A0A0X2I0_9SPIO
G5KHN3_9STRE

E3EKM5_PAEPS
C7MPK4_CRYCD
A0A0G0I018_9BACT
D6CK72_XANAP

F9MV75_9FIRM
A8YW48_LACH4
E1L0M3_9ACTN
A0A0F0KZA8_9MICO

B6GAM6_9ACTN
L0KBU6_HALHC
F8EJI2_RUNSL
B2TRG6_CLOBB

A0A0G3WHL9_9BACT
A0A0A2TPR6_9BACL
A0A0M2CX95_9MICC
R6M0Y4_9CLOT

A0A0B4RFI6_9BACL
C5RA89_WEIPA
H8E981_9MICO
D4YSR8_9LACO

G7QC93_9DELT
A0A0I9Y3H7_9MYCO
D5H0C0_LACCS
C9A8S5_ENTCA

R5CPV5_9BACT
C9ZFB4_STRSW
R5SAB4_9GAMM
D3F3T6_CONWI

U5S6H1_9LACT
SSB_TREPA
B2TP00_CLOBB
W9ELJ4_9LACO

A0A0M3D3N6_9MICO
K7VWI7_9NOST
R7FKB8_9CLOT
H6RVY1_BLASD

A0A0A7FWL2_9CLOT
A0A0M2U8X9_9FIRM
Q2RM71_MOOTA
A0A0M2VQ29_9BACL

X2HCF9_9GAMM
U2Q8L6_9FIRM
H5XC54_9PSEU
R5PVH7_9CLOT

Q74M27_LACJO
X2GVH9_9BACI
A0A0D5AEV9_9NOCA
R5Q9E2_9FIRM

Q2JQ03_SYNJB
S2DXU5_9BACT
K1ZW69_9BACT
B1MMI1_MYCA9

R6XQU8_9FIRM
R5AUK9_9FIRM
K6C9N5_BACAZ
K2EUR8_9BACT

C0MEM0_STRS7
E3IZT7_FRASU
D7GH49_PROFC
R6U666_9CLOT

C7XXJ4_9LACO
S1RPU4_9ENTE
C8WIP6_EGGLE
R5HK63_9FIRM

W5WNL7_9PSEU
R7L882_9BACT
E0RXF1_BUTPB
F5YVQ0_MYCSD

R7NEZ3_9MOLU
D4S6J8_9FIRM
R6NRQ8_9FIRM
R7NN10_9FIRM

R5CX84_9FIRM
A4FR31_SACEN
B0SSW1_LEPBP
A1RDC5_ARTAT

A0A0M2ZFM8_9MYCO
R6ECK9_9FIRM
R6NDS1_9CLOT
B1C975_9FIRM

S0SMI5_ENTAV
K9X4B7_9NOST
Q6A5N0_PROAC
A6X3T2_OCHA4

D3P9P9_DEFDS
D6E866_9ACTN
F2J196_POLGS
R5ME01_9MOLU

R2Q3W5_9ENTE
A1THY6_MYCVP
B1I6T0_DESAP
K2AEK0_9BACT

R5Y805_9MOLU
Q01FS1_OSTTA
B0T9B6_CAUSK
V5XKD4_ENTMU

R6UV64_9FIRM
A0A0F2C1D9_9MICO
R7D361_9ACTN
A0A0A1CZK3_9MICC

S0EYS7_CHTCT
R6B7C1_9CLOT
D9T411_MICAI
R5THR6_9CLOT

E9SVY8_RHOHA
A3DHF9_CLOTH
C0GI94_9FIRM
A0A0F5NGW8_9MYCO

D3IAG1_9BACT
Q0FUG1_PELBH
B9DJE5_STACT
A0A0F0LDG7_9MICO

A0A0F0KNI0_9MICO
R6H510_9FIRM
A0A0G3XKH1_9SPHN
A0A0A7PS75_9SPHN

R7ANG4_9ACTN
R7M6R0_9CLOT
C8WV95_ALIAD
R6DRY6_9CLOT

SSB_MYCTU
R5FLX6_9BACT
A0A0E9MUR3_9SPHI
W8TJI2_EUBAC

A0A088ET40_9SPHI
R6LVK0_9FIRM
A4RRU9_OSTLU
F8HY91_WEIKK

A0A068NVF8_9BACT
S2W6P3_9ACTN
E8X1F5_GRATM
K6VPE8_9ACTN

F1SRQ7_PIG
H1PJB0_9FIRM
K0Z6V8_9ACTN
G9YJ09_9FIRM

F2I5E0_AERUA
U4Q9S1_TEPAE
I2EVM7_EMTOG
I5AVT5_EUBCE

A0A086YV23_9FIRM
H1Z8W1_MYROD
A0A0M2RD12_9ACTN
E8SHY8_STAPH

R6GTK3_9FIRM
R7N7X3_9FIRM
E2RST4_CANLF
A0A0F5P9A7_9SPHN

A3DF53_CLOTH
B2TSA5_CLOBB
K9Y789_HALP7
M3ZQC4_XIPMA

B1X1C2_CYAA5
D3EPD2_ATETH
G9L570_MUSPF
F1N1S0_BOVIN

F1T5Q2_9ACTN
F6RJN1_HORSE
A0A0C2TU04_BACBA
A0A0J6YHL0_9MYCO

G1M9E9_AILME
A5VHG0_LACRD
Q744V5_MYCPA
C4FAZ9_9ACTN

F4CXT6_PSEUX
F6CF29_LACKZ
F7MSD7_CLOBO
L9L0W8_TUPCH

G4KXN0_OSCVS
D9QUL1_ACEAZ
H0XFVO_OTOGA
K7E8E7_ORNAN

B8I3W7_CLOCE
I0LDK8_9ACTN
I4B8K2_TURPD
E0NIS3_9FIRM

F7C414_ORNAN
G3T7N4_LOXAF
I0ILW6_LEPFC
R6Z1V7_9ACTN

R6WMS7_9FIRM
R7G0F0_9FIRM
E8RVU3_ASTEC
K6WB53_9MICO

D1PJS6_9FIRM
K9XY78_STAC7
A4XDG7_SALTO
R5WIJ2_9DELT

R5QD10_9FIRM
R5T0D9_9CLOT
R6DCZ8_9FIRM
C8W049_DESAS

D9SWF8_CLOC7
A8MED5_ALKOO
E7AD95_HELFC
R4K5X2_CLOPA

I3EBG5_BACMT
R5DJN3_9FIRM
A0A0F6R208_9CORY
R6HES5_9CLOT

W5PX87_SHEEP
D9VB97_9ACTN
F8EXJ2_TRECH
K9T956_9CYAN

K8GNK7_9CYAN
C7RH78_ANAPD
A0A0M3AIU4_9SPHN
K4IG43_PSYTT

Q46II8_PROMT
A0PX88_CLONN
R7AXP0_9FIRM
SSB2_LISMO

L8ALM1_BACIU
SSB1_SYNY3
D2BED7_STRRD
G9WF10_9LACT

H2J429_MARPK
R4M2D8_9ACTN
U3J0M0_ANAPL
U5WCD4_9ACTN

R5VMD3_9FIRM
Q03D46_LACC3
C4ZDL5_EUBR3
H2MQE2_ORYLA

R6BYC9_9CLOT
R5EAX7_9FIRM
R5T2Q1_9CLOT
R7R157_9FIRM

A4J9Q4_DESRM
A8UWG2_9AQUI
K9W323_9CYAN
D1BGE6_SANKS

A0A0D8I5T9_9CLOT
S1NV36_9ENTE
I0YSZ5_9CHLO
M1S8U3_MORMO

M1S8U3_MORMO
S1NK24_9ENTE
A4AEU3_9ACTN
SSB_MYCLE

G3Q9G8_GASAC
K0NMK9_9LACO
R7DBY1_9FIRM
F9DY26_9BACL

R6DN65_9CLOT
C9LU67_SELS3
R5TRH8_9FIRM
M7AI56_CHEMY

Q31RI6_SYNE7
B7KF71_CYAP7
A3K4Q5_9RHOB
D7VRI1_9SPHI

C8WYC0_ALIAD
G1TFS5_RABIT
A0A0G0M5P2_9BACT
D4J7E9_9FIRM

A0A087Y2Y2_POEFO
B1MWV1_LEUCK
G8T9U4_NIAKG
R6ERI5_9FIRM

A0A0D6A0Q8_9LACO
F6DRE2_DESRL
R7GDI2_9CLOT
A0A076NIV4_9CORY

F7KMP1_9FIRM
K4Z6N1_PAEAL
F0RVP0_SPHGB
D5BC93_ZUNPS

F6YE54_CALJA
R5P5H2_9BACT
R6ZJ99_9FIRM
K1ERU3_9MICO

A0A0M2UA92_9FIRM
D4JEM5_9FIRM
W5K2H1_ASTMX
K1YBF9_9BACT

A1HSI9_9FIRM
R6FJ83_9FIRM
R7J855_9FUSO
G5JXG8_9STRE

C8XDE2_NAKMY
C2L0W5_9FIRM
E1W135_ARTAR
G8QXZ1_SPHPG

K0YPC2_9ACTO
SSBP_RABIT
H0ZMY7_TAEGU
G1SKF0_RABIT

E7GNQ2_CLOSY
G1TWH4_RABIT
E3PS07_CLOSD
A0A0F0H116_NOCAE

H2U9B8_TAKRU
U2KG41_9STRE
A0A0G0CQI7_9BACT
A0A0B5D669_9CORY

R5DSD5_9FIRM
A0A0B5DZS3_9RHOB
A4XJ53_CALS8
G1SAX7_NOMLE

W5YBI8_9CORY
E8LE33_9FIRM
A0A0F2NNB1_9FIRM
R5TE43_9FIRM

A0A0F9Z9P0_9BACT
U3JMD2_FICAL
C3PIE7_CORA7
A0A0A2U240_9BACL

M3W650_FELCA
F4LSB8_TEPAE
C7HV65_9FIRM
R5BGZ2_9FIRM

C7Q4G0_CATAD
A5VH17_SPHWW
E1R210_SPISS
K9UNF6_9CHRO

F0H1S8_9FIRM
F3Y7N0_MELPT
C7N448_SLAHD
I7KKM2_9LACO

A1HR42_9FIRM
R5H238_9SPIR
E6S7C6_INTC7
W5TTM8_9NOCA

R5KQL9_9FIRM
R6K193_9FIRM
R7HRX6_9CLOT
R5ZCW9_9ACTN

C0R077_BRAHW
R5AJ65_9FIRM
K1XZ04_9BACT
A0A0D9R6X7_CHLSB

I2F404_9BACT
I3M8X8_ICTTR
A0A096MTD7_PAPAN
F6X3C0_MACMU

G7P1E9_MACFA
W8U9E7_EUBAC
I0X6L5_9SPIO
E6SLI9_THEM7

SSBP_PONAB
H2QVI4_PANTR
SSBP_HUMAN
G3R2E1_GORGO

R6PGX1_9FIRM
R6Q6X2_9FIRM
B4WRL5_9SYNE
SSBP_BOVIN

B0RDN7_CLAMS
I3KN30_ORENI
F7MJE1_CLOBO
A0A0G0J791_9BACT

R5ADK0_9FIRM
H3NIJ2_9LACT
A0A0F0LRP6_9MICO
R6HA90_9ACTN

Q7NCN6_GLOVI
E7EUY5_HUMAN
C8NI09_9LACT
K9S5Q5_9CYAN

R5U6P8_9FIRM
E3HXL9_ACHXA
SSB2_CLOAB
A0A0C7NPU9_9BACT

A0A0F3FVR6_9LACO
A0A022LVI6_9MICO
C2CYC8_LACBR
I7KJX8_9CORY

R6SP51_9FIRM
D0L1A3_HALNC
D4M166_9FIRM
A0A0G1YPM3_9BACT

R5SNS5_9FIRM
J9H7C5_9ACTN
R5ZLV4_9FIRM
SSBB_BACSU

L8AVU2_BACIU
F8E7P3_FLESM
G1QPH4_NOMLE
A0A0F4L1N9_9LACO

B8A5I7_DANRE
R6C2Z2_9CLOT
B9E7S6_MACCJ
F9Q480_STROR

E8V197_TERSS
I7LFZ3_9CLOT
R7C795_9CLOT
A0A0G2JLD8_HUMAN

R5CH32_9FIRM
M5A794_9ACTN
B0CEQ2_ACAM1
D3DG88_HYDTT

W1SK53_9BACI
C1F4R9_ACIC5
R6AVI9_9FIRM
E3PVY2_CLOSD

R5HBE8_9FIRM
E8N9Q1_MICTS
I0XMM5_9LEPT
K2BN93_9BACT

A0A0F2PZW6_9FIRM
F7K7Z8_9FIRM
D4LNF3_9FIRM
A0A089ZK63_9LACO

E1R6X0_SPISS
G1P8K1_MYOLU
B6GDU1_9ACTN
C1F5R8_ACIC5

F7V2Z0_CLOSS
C6LLT2_9FIRM
R7AVA4_9FIRM
R5DTA3_9FIRM

D4W5D5_9FIRM
R5MZ80_9FIRM
U5Q7H7_9BACT
R7CDZ3_9FIRM

A8UZL7_9AQUI
B2A455_NATTJ
R6E0A2_9FIRM
A0A0N8JYM3_9TELE

W0JDR4_9BACT
R6WEN5_9FIRM
G2SPI9_LACRR
B7C9Y1_9FIRM

I0GI75_CALEA
R4K4U6_CLOPA
G6F027_9PROT
R5HV83_9FIRM

R5FJB4_9ACTN
K8EIA4_9FIRM
R7K0K6_9CLOT
R7FFT2_9FIRM

B5CQ72_9FIRM
E0UMC2_CYAP2
F3ANA9_9FIRM
C7RGC0_ANAPD

R4SXQ7_AMYOR
F9ED05_9ACTO
D9VNU3_9ACTN
K9SIA7_9CYAN

R6PPR1_9FIRM
A0A0F5P757_9SPHN
D9R1Y1_CLOSW
A0A0M3AMT0_9SPHN

E1BTE0_CHICK
A0A0F0CNJ0_9CLOT
G9RVU9_9FIRM
A5Z4B4_9FIRM

F9ZPK3_ACICS
T1ZBR8_STRIT
D9XNX1_9ACTN
B0K8G3_THEP3

R5VQV8_9FIRM
K2GHD5_9BACT
G9PGJ7_9ACTO
E0UA33_CYAP2

A1WDQ0_ACISJ
A0A0M2H829_9MICO
Q1NC23_SPHSS
G2IU50_9SPHN

D4YY34_SPHJU
D7E202_NOSA0
A0A0M2NCL8_9FIRM
H9UKK5_SPIAZ

K9R8A5_9CYAN
I7J5V6_9CLOT
K9WD85_9CYAN
K9PEK3_9CYAN

A0A099WEX7_9LIST
SSB3_CLOAB
C3XP75_9HELI
A0A059MK36_9NOCA

K1M765_9FLAO
D6S3J9_9LACO
Q65E30_BACLD
R9YIK7_9PROT

F2IIU8_FLUTR
Q9CFP4_LACLA
Q4C4A2_CROWT
G9WKY9_9FIRM

F9PDK0_9STRE
A0A068SFW7_9FUNG
Q82YK1_ENTFA
A0A023WZ22_9ACTN

J4UBY6_9FIRM
V5XV83_ENTMU
R6D7W9_9FIRM
D4J8W6_9FIRM

F7UXA5_EEGSY
Q1GW29_SPHAL
R5X759_9FIRM
C7H6E3_9FIRM

A0A0F4JZ00_9ACTN
Q8DNH4_STRR6
D2NQ89_ROTMD
G0FZG1_AMYMS

A0A0G1DY79_9BACT
R6DY76_9FIRM
M1E510_9FIRM
F9PCR2_9STRE

D6YAJ5_THEBD
Q03UT2_LEUMM
D3D6I3_9ACTN
F9PZY5_STROR

A5UU58_ROSS1
A0A0F0ESI1_9MICO
A6LKQ0_THEM4
R4K910_CLOPA

A0A0F4LYD6_9LACO
R5A082_9CLOT
SSB_LACPL
C8WNE4_EGGLE

D6E8L3_9ACTN
K7RSG7_PROA4
R5HIX1_9MOLU
R5R0I9_9FIRM

D6E9A6_9ACTN
R5FWS9_9FIRM
F3ALU5_9FIRM
G4L9D5_IETHN

C9ZAN4_STRSW
R7BN60_9ACTN
A6EHG8_9SPHI
C4ZBQ4_EUBR3

A6TRB9_ALKMQ
D4M0T8_9FIRM
E8V518_TERSS
F5WV62_ERYRF

R7MTA7_9FIRM
B7K610_CYAP8
U3GW76_9CORY
U5L431_9BACI

A0A0A1H454_9BURK
B1XLH4_SYNP2
A7HKU6_FERNB
D3SNS2_THEAH

L5KQV0_PTEAL
K1WZW4_9BACT
A0A0F7HHS8_9STAP
R5BRV9_9FIRM

R5EDC6_9CLOT
R6JYD3_9CLOT
E6WYD3_NITSE
R5JVR8_9FIRM

D4LX16_9FIRM
Q7V9P5_PROMA
SSB_CALS4
G2THW2_BACCO

A0A0G1ZRH2_9BACT
SSB_THEEB
E4TF90_CALNY
B4U7M9_HYDS0

I0HJX3_ACTM4
A0A0G2K747_RAT
Q08D46_XENTR
G3V7K6_RAT

E6U6D8_ETHHY
D4MTT0_ANAHA
A0A0M2H3M8_9MICO
R7BHP7_9FIRM

R6HJ30_9ACTN
SSBP_RAT
R6G7P4_9FIRM
F6ZGI9_XENTR

R7FPV5_9FIRM
K9SYX5_9SYNE
D4YHA4_9LACT
K9VAE1_9CYAN

SSBP_MOUSE
C4Z4Q5_EUBE2
R7H9I6_9FIRM
E1M8N3_9STRE

D9S186_THEOJ
R7DAW4_9ACTN
A4J362_DESRM
U2EED0_9BACT

G3HGL0_CRIGR
R6HDB5_9CLOT
Q8R2K3_MOUSE
A9AYW2_HERA2

G2P799_STRVO
R7A8H1_9CLOT
R6Z8U9_9CLOT
A0A088T1M9_9MOLU

R5QXZ4_9FIRM
B2TMX5_CLOBB
B2J2S0_NOSP7
B1H012_UNCTG

A0A0A0X352_9SPIO
E8JIW4_9ACTO
D2S7J2_GEOOG
G4L1H2_OSCVS

H0QKG9_ARTGO
K9ZC28_ANACC
F5SHA9_9BACL
D2RN22_ACIFV

B1C4J5_9FIRM
K0J1F5_AMPXN
C5ESW7_9FIRM
G4D4Z7_9FIRM

R4KD06_CLOPA
R5ZLD1_9FIRM
R5C772_9FIRM
E9T0A1_RHOHA

K6DEM7_9BACI
A0A0A1DSS4_NOCSI
C6Q0F7_9CLOT
R5FA57_9CLOT

R4TJ11_AMYOR
D4MX05_ANAHA
R6DH82_9CLOT
Q7UZN5_PROMP

A8TCG9_9VIBR
R5P3I4_9FIRM
D4L214_9FIRM
D4LVA5_9FIRM

B7C9D1_9FIRM
Q8XNP5_CLOPE
U5RXM5_9CLOT
B5GXB2_STRC2

W0EY94_9SPHI
R6NH05_9FIRM
H5TVR1_9ACTN
H6N236_GORPV

Q4L5H4_STAHJ
A1AY96_PARDP
K9QNQ4_NOSS7
R5I4T8_9FIRM

R5U7M0_9FIRM
A0A0E3M802_CLOSL
A0A0H3J879_CLOPA
D4YKW8_9MICO

SSB1_LACLA
A6TJA8_ALKMQ
R5SUP9_9CLOT
D0UIR6_AGGAD

A0A062XK97_LEUPS
SSB_STRR6
A0A0F9YFD2_9BACT
V6K9R3_STRRC

R5QDS7_9FIRM
A0A0M2PPY3_PROHO
F2N9C8_CORGP
A0A0C1T6X8_9ACTN

A9KKC9_CLOPH
R6IDK3_9FIRM
V4ITH1_9ACTN
E8SDU6_STAPH

F5XIS8_MICPN
R6PZ59_9CLOT
K0B4K0_CLOA9
E1QXP3_OLSUV

G5B580_HETGA
J5HB87_9FIRM
G1KLA3_ANOCA
B8E0J1_DICTD

G3VF75_SARHA
A0A075TTH1_9CORY
B5Y875_COPPD
A5I800_CLOBH

G3IU60_9GAMM
E6U5C2_ETHHY
R5K868_9CLOT
R6M217_9CLOT

G7GKY9_9ACTN
H7C6V9_ENTFA
A0A0F5VSZ8_9ACTN
Q17Q33_AEDAE

C3X7T9_OXAFO
R7B0S7_9BACE
V9XJ27_9NOCA
G5GFZ2_9FIRM

L0KAK0_HALHC
A0A0C5FYX7_9ACTN
B9EBP8_MACCJ
C7MB48_BRAFD

C4GC71_9FIRM
B0T9Q9_CAUSK
A0A0F6TCB0_9CORY
R4K1E0_CLOPA

R6JMM3_9CLOT
G5K4M6_9STRE
G4L3L8_TETHN
I0AH49_IGNAJ

B5GHS9_STRSH
Q1WVN8_LACS1
A0A0G3XK67_9SPHN
I3WC09_THESW

U7L398_9CORY
R6FHU1_9FIRM
Q6ABW8_LEIXX
R5UU53_9FIRM

A0A0H4C5K0_9ACTN
F9VBM4_LACGL
H1BI13_9FIRM
R6SCI7_9FIRM

SSB_CLOTE
D9QD46_CORP2
G9ZQA9_9LACO
D4K3Q1_9FIRM

R7R317_9FIRM
A0A078KJD7_9FIRM
Q2G694_NOVAD
A0A0M3AM79_9SPHN

F0YW24_9CLOT
V6DFC0_9DELT
K2GY73_9BACT
F8E3G1_CORRG

N2BLT6_9ACTN
Q8F5K5_LEPIN
Q2NBL0_ERYLH
F6ENV5_AMYSD

C7H6V9_9FIRM
R7HZX7_9CLOT
A5F9Q3_CLOK5
R7G9H7_9FIRM

D4L1G5_9FIRM
F6VVL1_MONDO
A0A0M3AYD6_9RHIZ
D4LM12_9FIRM

K0KGN7_SACES
K1LY02_9LACT
SSB1_NOSS1
A0A0A8B1Q7_9ACTN

R6BI07_9FIRM
A0A0G3AI68_9ACTN
C0CTZ5_9FIRM
E6UEW0_RUMA7

U5MXG3_CLOSA
D4CKG8_9FIRM
D7B132_NOCDD
C0C5L8_9FIRM

A0A0D4DPL9_9ACTN
H2RHH4_PANTR
C9K0U8_HUMAN
A3TN96_9MICO

F0H1Q3_9FIRM
R5D149_9FIRM
D3D9X6_9ACTN
D7N542_9FIRM

Q6NEQ1_CORDI
A0A091DHX3_FUKDA
D0BLJ2_9LACT
A0A0F0HG07_9ACTN

W6S615_9CLOT
K0YVT6_9ACTO
T0TUT1_9STRE
I5C950_9BACT

R5YEH2_9FIRM
K9Q960_9NOSO
K9U3S1_9CYAN
A0A073CAT4_PLAAG

C7M1H0_ACIFD
Q2J4C5_FRASC
A0A0M2XVT1_9SPHI
Q9CIG8_LACLA

T1ZFH7_STRIT
A0A075KIH8_9FIRM
L8EF91_STRRM
F8JTF2_STREN

Q01P66_SOLUE
A0K2H4_ARTS2
E7NQ91_TREPH
R5X9Q1_9CLOT

J6HF95_9FIRM
C3X998_OXAFO
J0UXX0_9HELI
M1NQN4_9CORY

A0A0M2JJ61_9ACTN
A0A0H3JAY7_CLOPA
A0A0G4AZY9_9BACT
Q81E38_BACCR

W0PCJ2_9BURK
R6QBY0_9FIRM
A0A075JDJ9_9MICO
F0SA65_PSESL

V8PCQ9_OPHHA
D5ZR07_9ACTN
G2T4Z3_ROSHA
U2IJE4_9STRE

F3Z785_9ACTN
J5UH88_9FIRM
R5ICL5_9FIRM
A0A0G0I4S4_9BACT

Q1B002_RUBXD
R5KD39_9CLOT
A0A0F4KNF1_9ACTN
B8D1C8_HALOH

C4XPY4_DESMR
I7KGV3_9LACO
A0A0G1V5K9_9BACT
F8I9E4_SULAT

A0A099WMF3_9LIST
Q4JSG0_CORJK
Q81R86_BACAN
Q1GC36_LACDA

E1M5B2_9STRE
E4KMD8_9LACT
A0A0G1PY19_9BACT
A0A0A1SB08_CLOSO

C2W7H2_BACCE
D8LMF5_ECTSI
R6QZ78_9CLOT
H6R954_NOCCG

L0RV23_MYCC1
I8RHV1_9FIRM
R7I585_9FIRM
A0YMQ0_LYNSP

R5AMA1_9FIRM
D9VGM5_9ACTN
A8MHY9_ALKOO
C6WSG2_ACTMD

C8WA62_ATOPD
A0A0F5YJ18_9CYAN
A0A0F0LWI2_9MICO
R5YE83_9FIRM

B4V9L5_9ACTN
A1B6V4_PARDP
R6C7H6_9FIRM
R6TQE9_9STAP

R6AYP4_9CLOT
F3NK91_9ACTN
A4VSM6_STRSY
J1F2K6_9LACO

Q2N5L5_ERYLH
B8HPH8_CYAP4
SSB2_STRA5
Q8DS68_STRMU

B9DT16_STRUO
H0V0N9_CAVPO
B0S4C2_FINM2
R6RS34_9FIRM

A5P8J7_9SPHN
SSB3_STRA5
H7C718_ENTFA
R6Z0P3_9FIRM

K8EJP0_CARML
E2S8L8_9ACTN
A0A0A8B2R2_9ACTN
G5IMP8_9CLOT

V6K1U9_STRNV
R7XTM4_9ACTN
H1LHE4_9LACO
C7PVD3_CATAD

B0PAM7_9FIRM
K2BW59_9BACT
V5RWF6_9BACT
E7GFL6_9FIRM

A0A0A8EN42_9ACTN
A9KQ32_CLOPH
C2GFP6_9CORY
A0A0F3K3Z3_9NEIS

V5XVL3_ENTMU
R5IVW3_9CLOT
Q2NDL0_ERYLH
M4SAH7_9SPHN

B0WQG1_CULQU
R5IVB6_9FIRM
A0A0G1R8N2_9BACT
E2ZG54_9FIRM

K9Z8A2_CYAAP
K9XBI7_9CHRO
C4LKJ5_CORK4
R7K9F5_9FIRM

R7GK56_9FIRM
D1A6N9_THECD
D8G2X3_9CYAN
F8B488_FRADG

A5N439_CLOK5
SSB_UREPA
X4QZF0_9ACTO
A0A0B4S0X3_9FIRM

R5J759_9CLOT
D6A5L5_9ACTN
G8SFP7_ACTS5
K1XM58_9BACT

S0RKA5_9ENTE
C5C660_BEUC1
A0ZBN5_NODSP
E2ZBE7_9FIRM

G1D5D5_9CAUD
D5UE91_CELFN
R7BU22_9FIRM
R5J3X1_9FIRM

R6AJ00_9CLOT
R7P3M7_9CLOT
R5I982_9BACT
A1B7E0_PARDP

A0A077JG98_9CYAN
G5KEQ7_9STRE
R5ITS9_9CLOT
SSB2_NOSS1

H2K3N5_STRHJ
A0A0H4BZY2_9ACIN
Q47K95_THEFY
C7Q5K4_CATAD

I3ZJ61_TERRK
A0A0G3XEQ1_9SPHN
D1PNB5_9FIRM
Q181R6_PEPD6

C0XQX6_9CORY
R4K0U4_CLOPA
R6JHK2_9CLOT
D4K2R3_9FIRM

A0A0F2G8L1_9ACIN
A0A0M2LZS7_9MICO
B3QVR6_CHLT3
D6ZJY0_MOBCV

C8PB10_9LACO
D4KCM1_9FIRM
R6ES87_9FIRM
D7C8S4_STRBB

A0A089Z0G9_STRGA
C9RC58_AMMDK
A0A0M2XMB8_9CORY
A0LWU4_ACIC1

A0A0B5D8I7_9CORY
R7K5T4_9FIRM
E1R3J5_SPISS
N0B1A9_9BACI

E0YPL4_9CAUD
Q116R3_TRIEI
C0E890_9FIRM
D1VUS3_9FIRM

K9X8U2_9NOST
E1R1F8_SPISS
R5XKF2_9FIRM
F6ILS3_9SPHN

B4W099_9CYAN
B8HJJ1_ARTCA
A8ZYK1_DESOH
A0A077HMZ9_9CORY

E5WGI9_9BACI
A0A0A8JKP8_BACSX
A3W9Z9_9SPHN
A0A0D6E047_9LACT

G0FLH8_AMYMS
A0A0M3DHT1_9CLOT
B0PHZ0_9FIRM
H1LEZ9_9LACO

A0A0F2TJA1_9ACTN
E0S4M7_BUTPB
K4ZGF4_PAEAL
A0A0H4C1Z5_9ACTN

G7MAP9_9CLOT
S4G5V5_GARVA
G5JV28_9STRE
A0A0F9Z0F5_9BACT

K9YM97_CYASC
Q82YU2_ENTFA
F9HJI2_9STRE
A8AZF8_STRGC

X5DQY1_9CORY
A3WDG7_9SPHN
C9RAG8_AMMDK
SSB2_CHLTE

B6GDJ1_9ACTN
U2DZE5_9BACT
A0A0A7FYU6_9CLOT
R6LLA3_9FIRM

F6CGA7_LACKZ
R5LYQ9_9FIRM
D4H166_DENA2
C4IMP1_CLOBU

K6U627_9CLOT
B9Y3H0_9FIRM
Q3Y114_ENTFC
I7JFJ5_9LACT

B0S2G8_FINM2
D9X019_STRVR
B4VV03_9CYAN
H1BPB1_9FIRM

K4QY06_9ACTN
A8KY48_FRASN
Q3B6M6_CHLL7
D3QYV9_MAGIU

H7EPC4_9SPIO
G0HB64_CORVD
SSB1_TROWT
K7SSA5_GLUOY

A6M304_CLOB8
Q857R0_9CAUD
Q5M2H8_STRT2
Q3ANR4_CHLCH

B4S3C4_PROA2
M4S4J6_9SPHN
J3JC89_9LACO
X4ZWN4_9BACL

R5AH99_9CLOT
R6R1Y2_9FIRM
Q2NBN4_ERYLH
C0W5T8_9ACTO

R6QI05_9FIRM
A0A0M2N1B0_9ACTN
B4NKZ7_DROWI
H4GIG3_9LACO

D4JTJ3_9FIRM
B0JQ04_MICAN
B4KA00_DROMO
R6ULN2_9FIRM

D4LDT4_RUMC1
D7BLE9_ARCHD
I3VSR0_THESW
R5KII9_9CLOT

A0A0M2GYF5_9MICO
J1ZWV2_9ACTN
E8X7E8_GRATM
K9RR91_SYNP3

G5K2B2_9STRE
A7GP66_BACCN
F5Z5L2_ALTSS
G6EMZ8_STRTR

B8H840_ARTCA
A0A084WRC1_ANOSI
R6Y2B4_9CLOT
B3MTU7_DROAN

L7LMG5_9ACTN
C8NI83_9LACT
B0PEV9_9FIRM
S5QY48_9STRE

D9W8L5_9ACTN
R5F117_9CLOT
U3JCL9_FICAL
B4SB43_PELPB

E3H7R3_ILYPC
A0A0A1GU37_9LACO
B4IBZ2_DROSE
SSB_CORGL

B4QX63_DROSI
SSBP_DROME
I7J8Z5_BABMI
E8WBV8_STRFA

E0E595_9FIRM
B4LXZ6_DROVI
G2G710_9ACTN
A0A0F2PDU0_9FIRM

T0UUE9_9STRE
E3H340_ROTDC
C5NVZ7_9BACL
C7XUW1_9LACO

A1BJC2_CHLPD
A0A075KAQ6_9FIRM
A1WDI7_ACISJ
R5FUF7_9FIRM

R7HLY7_9FIRM
D4LEZ6_RUMC1
SSB2_STRCO
R6JNI5_9CLOT

R5MMP1_9FIRM
W7TIN7_9STRA
L8F0E9_STRRM
B4GMJ5_DROPE

Q294W1_DROPS
E6KSY5_9ACTO
G0FHG4_AMYMS
Q7QC69_ANOGA

K2D879_9BACT
K1LWN4_9LACT
A0ZFI4_NODSP
C7NDG9_LEPBD

K2F787_9BACT
Q0RAR5_FRAAA
Q48AK8_COLP3
E0E1I1_9FIRM

D1A8E9_THECD
R7HIN3_9MOLU
R6MZ43_9CLOT
C4WSH6_ACYPI

B4JG16_DROGR
U2N105_TRESO
Q6NEI4_CORDI
A0A0L0C938_LUCCU

D6K6Y6_9ACTN
E0UNQ0_CYAP2
A0A075KE67_9FIRM
R6UNN6_9CLOT

M1N7D9_9CLOT
R5VJF6_9FIRM
Q0YNZ3_9CHLB
A0A077HLA7_9CORY

R5BQM1_9FIRM
B8I053_CLOCE
J1H3J1_9CLOT
SSB4_STRA5

M1CAV8_SOLTU
U2KMT7_TRESO
F0S465_DESTD
I7A1K0_MELRP

G0G277_AMYMS
A0A0G1KEH6_9BACT
SSB_AQUAE
R6RG83_9FIRM

M1CAV7_SOLTU
A0A0C5S293_9MOLU
R7FWV6_9PROT
E3H1M9_ROTDC

I9LJM8_9FIRM
A0A0F5I6R0_9BACI
SSB_LEPIN
U4KR14_9MOLU

F4GH00_ALIDK
E7MR23_9FIRM
A0A0C2YAV4_BACBA
K0YNW0_9ACTO

G8SF46_ACTS5
K4B142_SOLLC
W5JL12_ANODA
R5VM87_9CLOT

A0A067R5S5_ZOONE
E8T4D8_THEA1
N2BLQ3_9ACTN
B5H606_STRPR

V5WCX2_9SPIO
C4F8E6_9ACTN
A0A059B337_EUCGR
A0A0B5AQD2_9BACL

A0A0A8W776_CLOSO
C4LGG0_CORK4
A7SEZ5_NEMVE
E0UNI7_CYAP2

F8JWV7_STREN
R6XRZ0_9CLOT
K6DKB5_BACAZ
A0A0M2Z6A1_9ACTN

K9ZS22_ANACC
R5JB91_9FIRM
B9STF2_RICCO
S8C5W4_9LAMI

G5K9Z6_9STRE
A0A0F5W5C6_9ACTN
D2A2D3_TRICA
Q8Y4C7_LISMO

U4KLQ5_9MOLU
C0MFZ9_STRS7
R6NWZ1_9CLOT
C7M1D3_ACIFD

B7GDH9_PHATC
R6LWH9_9CLOT
U2Q8T2_9FIRM
F8I2Y8_SULAT

A0A077J6V4_9BACI
R6YDA7_9CLOT
D6Z9K0_SEGRD
M0TZ66_MUSAM

W4WGZ6_ATTCE
U5DC96_AMBTC
A0A0F7N7R7_9ACTN
A1RC69_ARTAT

G6FVY8_9CYAN
R5Y2C2_9CLOT
R7MDV0_9CLOT
G1NKN7_MELGA

B0SP31_LEPBP
B0S487_FINM2
A0A0A1CW74_9MICC
R5TB82_9CLOT

R5GS36_9FIRM
B3R0K2_PHYMT
B3EKG0_CHLPB
R6FRM9_9CLOT

Q8R8F1_CALS4
A9T9Z2_PHYPA
D0GIJ6_9FUSO
G7I9F0_MEDTR

A0A0G3HFP3_9CORY
I0XTS8_9LEPT
R6E141_9FIRM
D6YRN8_WADCW

Q6YRK4_ONYPE
E1R7U9_SPISS
F2JRY8_CELLD
C4GAT4_9FIRM

I1A6Q6_9MOLU
A9HT87_GLUDA
F9PLE9_9ACTO
E2A4E2_CAMFO

J7L8W1_NOCAA
A0A0F7CP49_9ACTN
R2S0D0_9ENTE
C6VZY4_DYAFD

H9IUP7_BOMMO
A0A072VNU3_MEDTR
I1CFQ3_RHIO9
K9U4I7_9CYAN

M2XDC8_GALSU
G2J0V5_PSEUL
A0A061ABV0_9MOLU
SSB_BORBU

D9WWZ7_9ACTN
B0S470_FINM2
J3JWK6_DENPD
A9NEP0_ACHLI

R5KNI9_9CLOT
E2C7L8_HARSA
A0A0M2HMR7_9MICO
A0A0G1VV93_9BACT

R6H4K2_9FIRM
F4XCR8_9FIRM
D7BCU9_MEISD
D7BCU9_MEISD

C4ICW4_CLOBU
D0WL44_9ACTO
A0A0M3DGJ3_9CLOT
D2VRD1_NAEGR

D0BM39_9LACT
D1AJZ4_SEBTE
R6MUJ6_9FIRM
B5I5T8_9ACTN

A1QYR3_BORT9
A0A022REF4_ERYGU
W5A3T7_WHEAT
S5LST4_9MOLU

K1YZ76_9BACT
T1G5H7_HELRO
B2TQY8_CLOBB
A0A0D6DYW6_9LACT

F4LPJ2_TREBD
W0PFC5_9BURK
M0TS40_MUSAM
M1M7M3_9CLOT

J9W2T3_LACBU
A6CPQ2_9BACI
I7L846_9LACT
W5AER3_WHEAT

A0A068NH60_9ACTO
J9W267_LACBU
U6E388_9MOLU
A0A058ZA14_9EUKA

F7ZZC6_CELGA
E8MXY3_ANATU
D7U9Z5_VITVI
I2N1Y8_9ACTN

K4ZCE8_PAEAL
A0A0B5EZL0_9ACTN
R0HD12_9BRAS
A0A061FAS7_THECC

C3WBN3_FUSMR
D1BRT7_XYLCX
A0A0D4CJ99_9LACO
E2ZNS8_9FIRM

G6DF00_DANPL
A0A061F3U5_THECC
A0A0G3XN39_9SPHN
D4BM44_BIFBR

R6I6X7_9FIRM
R7BIL2_9FIRM
A0A087GPT5_ARAAL
G5KEE4_9STRE

S2K7U9_MUCC1
M5WHL0_PRUPE
D4MVL9_ANAHA
I3UGJ2_ADVKW

F2EFX4_HORVD
G2NXT3_STRVO
I1BS51_RHIO9
F4WAN6_ACREC

F8L069_PARAV
A0A0A0LVY2_CUCSA
R5MS63_9MOLU
A4JV78_BURVG

E0DH66_9CORY
A0A023CPK7_GEOSE
F4GK80_SPHCD
A0A0A7I4Q3_9BIFI

I6XYA9_PROPF
SSBP_ARATH
R6H221_9FIRM
E2ZAS2_9FIRM

K0SQT6_THAOC
E4RL94_HALHG
A0A067L436_JATCU
F4H5G5_CELFA

D5AVD7_RHOCB
G2NI88_STREK
A0A0K9NWZ7_ZOSMR
F9MRH9_9FIRM

K1M9H1_9LACT
A0A061E958_THECC
M0XSF7_HORVD
A4XE47_NOVAD

K2EF75_9BACT
E3IUL2_FRASU
E4PRR2_MARAH
R6R906_9FIRM

D9WPL2_9ACTN
A0A0G0T216_9BACT
A0A0M2HGC3_9MICO
SSB1_CLOAB

E8NDK7_MICTS
J0NGW5_9ACTO
T1L9G1_TRIUA
A0A0A7I9B8_9BIFI

Q6MC71_PARUW
A0A0D3G9M1_9ORYZ
A0A0E0PPT0_ORYRU
A0A0E0A1G2_9ORYZ

R5HKG1_9MOLU
F8L7J6_SIMNZ
U7MT71_9CORY
F2ICX2_FLUTR

V4QNQ3_STRIN
F2RD73_STRVP
A0A0A8EXI3_9ACTN
D7EYW0_ARALL

D4BRU5_BIFBR
W4ZRU4_WHEAT
C4IYS0_MAIZE
I4YF78_WALMC

A0A0G3HFE7_9CORY
A0A0E7LNC1_9NEOP
R5A412_9CLOT
I1EB73_SOYBN

I1QKS2_ORYGL
Q6E4W0_ORYSJ
B9I7A1_POPTR
A0A0A7PEE5_9BACT

K3Z9P1_SETIT
C6VVF4_DYAFD
D1NWJ3_9BIFI
A0A097IIX8_9CORY

V7BK76_PHAVU
A0A0E0L4I0_ORYPU
G9WE70_9FIRM
A0A0D3E947_BRAOL

A0A096QAV3_MAIZE
K2BAD0_9BACT
A0AW08_ARTS2
A0A0M2HYL8_9MICO

R6BKT7_9CLOT
I0GQE5_SELRE
R6GPN1_9FIRM
F2NT26_TRES6

H8E4K3_9MICO
I4EAU6_METSZ
B1VPC3_STRGG
R6CL18_9CLOT

M4F2F9_BRARP
D7W9B0_9CORY
A0A0H4P3T7_9BACI
D7BJJ9_MEISD

C2WZR6_BACCE
A0A078DHL3_BRANA
E6W6W1_DESIS
I1HHT5_BRADI

A0A0M2EPP6_9SPHN
D2Q3W6_KRIFD
R6Z1D4_9FIRM
K7J2W0_NASVI

C4K426_HAMD5
B8AZT8_ORYSI
E3DS65_HALPG
A0A0D2R9N2_GOSRA

A0A0D2R335_GOSRA
C4GHF6_9NEIS
A0A0D2S506_GOSRA
I1HS48_BRADI

C7R3A8_JONDD
H3NE41_9FIRM
K9U8Y9_9CYAN
F8AYN8_FRADG

A0A0F0HK54_9PSEU
R6E0F3_9FIRM
A0A0F5P9A5_9SPHN
D4K451_9FIRM

A0A059NWA3_9BACI
A0A0M2UAZ0_9FIRM
H2CBY9_9LEPT
A0A078JKM8_BRANA

R6D5E3_9CLOT
A0A0D2PHD8_GOSRA
C6JRG5_FUSVA
B9EBK8_MACCJ

J3M8R3_ORYBR
E9SD49_RUMAL
A0A016PN37_GIBZA
A6WG62_KINRD

R5W069_9FIRM
A1A3I0_BIFAA
E5BEX3_9FUSO
R4KEJ9_CEOPA

I3WG37_BIFBI
D9SSV4_CLOC7
A0A0H4L230_9BACI
I1LB74_SOYBN

A0A0F7JQ78_9SPHN
A0A0C9M3T0_SPHPI
B2KEG1_ELUMP
I1HS47_BRADI

D4BUG1_PRORE
D4BUG1_PRORE
R7BP32_9ACTN
R5TEL9_9CLOT

R5VZQ8_9FIRM
A0A077RTF1_WHEAT
A4F8T1_SACEN
BIV954PHYAS

F8J185_9CAUD
D1AW03_STRM9
W5D8T8_WHEAT
R5J8V9_9CLOT

Q21QI3_RHOFT
A0A0E0KPE8_ORYPU
I1YEN3_METFJ
W9R5P5_9ROSA

W9R5P5_9ROSA
W9S301_9ROSA
A0A068R5E5_9ENTR
K6TTT2_9CLOT

K3VSP0_FUSPC
S5SQD8_9PROT
R5TNB7_9CLOT
A0A0G3XNP5_9SPHN

Q6YQ11_ONYPE
E7NXU2_TREPH
D2Q6Q0_BIFDB
A0A0M2H5S7_9MICO

D1ANB3_SEBTE
A0A0A0LTL6_CUCSA
B8C9K7_THAPS
A0A0M2EMI0_9MICO

B7Q357_IXOSC
D8RH51_SELML
D8RAR6_SELML
A0A0F2T3P0_9ACTN

Q6YPM6_ONYPE
D3CUK6_9ACTN
SSB_FUSNN
E7VKB8_CEOSH

F9EEE1_FUSNU
W4KF50_9HOMO
M0SIR5_MUSAM
MTSS1_CAEEL

J3E2A2_ORYBR
A0A0D9WIG4_9ORYZ
S5YJB8_PARAH
B6JK37_OLICO

A0A0D3FUN2_9ORYZ
Q2NV17_SODGM
W5WTH1_9CORY
F9VI82_ARTSS

A0A090JPR1_9FIRM
K4IZG0_BIFAP
A8X3C7_CAEBR
G0MGH4_CAEBE

V4L410_EUTSA
K7N5B5_SOYBN
R6AMR3_9CLOT
Q6YQE4_ONYPE

H1D838_9FUSO
Q6YPJ9_ONYPE
Q1QFG2_NITHX
E3N038_CAERE

I2GU76_9BACT
D6YW20_WADCW
V5FI32_BYSSN
A0PZ40_CLONN

E9IM03_SOLIN
K0JPX9_SACES
Q6A791_PROAC
A0A077KLD4_9FLAO

F8KZF0_PARAV
A0A090LE13_STRRB
R5MQA9_9FIRM
C5NV94_9BACL

E3D770_GARV3
F0RTU0_SPHGB
X2GTB7_9BACI
I1NJ41_SOYBN

R5BXI6_9BACE
A0A088T2E7_9MOLU
R5IVN3_9FIRM
A0A0E9LYC1_9BACT

R7EPH8_9BACE
A0A0D9MH74_9EURO
R4ECR5_9ACTN
R7HH66_9MOLU

Q6YQH7_ONYPE
A0A088A2R7_APIME
F9DX54_9BACL
Q6YQ63_ONYPE

C8NHW9_9EACT
D3PMG9_MEIRD
F2F3Y0_SOLSS
J3EX07_ORYBR

K3XLP5_SETIT
M4RAQ1_9BIFI
A9TAN1_PHYPA
R9ACF2_WALI9

A0A088QJJ4_9CORY
A0A059VZJ4_STRA9
W5CFS3_WHEAT
A0A0F0I7X5_ASPPA

R6B869_9CLOT
A0A0G3GUQ1_9CORY
E7MQM1_9FIRM
B5JNU0_9BACT

A0A0D9WIG3_9ORYZ
G1XDC6_ARTOA
I9C2C6_9SPHN
U6PK61_HAECO

G0HI79_CORVD
A0AWV3_ARTS2
A2WT49_ORYSI
B8PEC7_POSPM

Q6YQK9_ONYPE
K3XM68_SETIT
B7KMH3_CYAP7
A0A0E0JLP0_ORYPU

A0A060SPG2_PYCCI
K2FDS2_9BACT
A0A0B5I737_9ACTN
R7R2A3_9FIRM

I2JIG7_9GAMM
A0A0M2CUY5_9MICC
B9YD18_9FIRM
A0A0D6T7V8_9RHOB

A0A0A7G012_9CLOT
Q24LE5_BPPCD
A0A067GSU9_CITSI
K0F5S9_9NOCA

D4H4K0_DENA2
K0F0D9_9NOCA
S8ANE7_DACHA
F6FJB9_MYCHI

A0A0G0D266_9BACT
Q184D1_PEPD6
A0A0K0CZ86_ANGCA
D2MMI2_9FIRM

SSB_MYCPE
A0A0F4JXZ9_9ACTN
S5LYV5_9MOLU
E5BDE2_9FUSO

R7HJM0_9FIRM
T0U3E9_9ENTE
V5RJ91_SPIAP
C4IGU4_CLOBU

A0A077RA69_WHEAT
A0A0F0KC91_9MICO
A9JQS8_ONYPE
R5K3H0_9CLOT

B1VIZ7_CORU7
J8VIP9_9SPHN
A5VGC6_SPHWW
W8GEA7_9MOLU

R5X4S7_9CLOT
V4SGF4_9ROSI
A0A067GQ74_CITSI
T1H2Z8_MEGSC

R5N754_9FIRM
E9HG91_DAPPU
A0A0J8BBZ0_BETVU
R7DQ35_9FIRM

J6H3K8_9PORP
K4DEI6_SOLLC
B8NEG5_ASPFN
W6NRG3_HAECO

A0A0L1JBF4_ASPNO
W6QPG1_PENRO
A0A0D9V3F9_9ORYZ
A0A061G1I5_THECC

E6JBU4_9ACTN
W5IGQ0_SCAIO
Q5VP75_ORYSJ
U1M323_ASCSU

Q2FWF7_STAA8
I1NQ20_ORYGL
A0A0D3ERR1_9ORYZ
A0A087DDF0_9BIFI

F8A0N0_CELGA
W9EFH9_9LACO
Q6YPS0_ONYPE
A0A088QF08_9CORY

Q6YRH4_ONYPE
A0A0F0L4E0_9MICO
A0A067NLS3_PLEOS
F7W361_SORMK

L0G3E7_ECHVK
A0A0D9YC93_9ORYZ
M5FQH0_DACSP
E6TS66_BACCJ

U3U3T1_9ENTR
G4UQY2_NEUT9
M7YFY9_TRIUA
Q6YQG3_ONYPE

A0A0G1MMF6_9BACT
B9RE51_RICCO
Q2U7U2_ASPOR
R6X8C2_9BACT

E5WQN5_9BACI
A4J7S4_DESRM
D2NTP1_ROTMD
M4E0N7_BRARP

T1KFW6_TETUR
U4KKW9_9MOLU
R5MG88_9MOLU
E6K1V8_PARDN

SSB1_STRAW
A0A0G3HKR4_9CORY
D7VT17_9SPHI
R6SIP2_9FIRM

A0A078DUV6_BRANA
C5XF43_SORBI
H9UM39_SPIAZ
E1VWT1_ARTAR

K6V5A9_9MICO
F2IIU7_FLUTR
B2GG11_KOCRD
D3NUP5_AZOS1

A0A0F2FYC5_9ACTN
G4ELW3_MYCIO
A9RL19_PHYPA
M5WD92_PRUPE

M0XU19_HORVD
W7IFB9_9PEZI
A0A0F8UQU1_9EURO
R6Y4Q4_9FIRM

H0DGD7_9STAP
A0A0F0KXD3_9MICO
S8C8U9_9LAMI
C0BP77_9BACT

I1K195_SOYBN
W7LZR9_GIBM7
I1HPU9_BRADI
Q5HMC5_STAEQ

G8QX45_SPHPG
W5XWC6_9CORY
B4FRF4_MAIZE
C4FDE6_9BIFI

W6KQ01_9PROT
D7U3W8_VITVI
A5HYT7_CLOBH
D4H4T7_DENA2

Q893X5_CLOTE
U1FKZ5_TRESO
A9SBZ1_PHYPA
K8E772_CARML

A0A0D9VWL3_9ORYZ
E0S4T0_BUTPB
G8T8J7_NIAKG
M5BIN7_THACB

A0A0E0KGK4_ORYPU
E6UKA6_RUMA7
B6HQ01_PENRW
C5CAV8_MICLC

J4H3J7_9APHY
A0A0B4REG2_9BACL
S8FNG6_FOMPI
A0A067FQ03_CITSI

A0A085BWI4_9RHOB
E4KMV6_9LACT
V4VMU2_9ROSI
A0A0B2UZX5_TOXCA

U5VVL1_9ACTN
A0A0F2C284_9MICO
A0A077EL86_9FLAO
F0ICS7_9FLAO

F2NUF3_TRES6
A0A078EES3_BRANA
B9E8E1_MACCJ
S0DXT4_GIBF5

V2XJ24_MONRO
U4KRJ4_9MOLU
D4ZVZ7_ARTPN
A0A0B5DNE3_9ACTN

A0A0K9PNM3_ZOSMR
A5FFM4_FLAJ1
D4YQV9_9MICO
G9ZFM2_9GAMM

U9W337_NEUCR
F8B2W0_FRADG
B9DMG3_STACT
A0A0G3H624_9CORY

C1N4Z6_MICPC
R7GJ76_9CLOT
J6CJM6_PASMD
M0XU18_HORVD

J3CM39_9FLAO
D8T9F3_SELML
F2L6K8_PSEUX
Q5WE28_BACSK

S5VQJ5_STRC3
H5UT03_9MICO
D7L8J7_ARALL
C1EJB6_MICSR

D1KBN3_9GAMM
D3LVH7_9FIRM
A0A0A0WYH3_9SPIO
R7M578_9CLOT

I1PDQ5_ORYGL
A2XJV7_ORYSI
A0A0E0NYJ9_ORYRU
Q75GK2_ORYSJ

N4U305_FUSC1
A0A0D2XE17_FUSO4
X0K659_FUSOX
N1RE73_FUSC4

K9GIC0_PEND2
A0A0M1V0K5_CLOSO
V3ZV46_LOTGI
R7FPD1_9CLOT

F9FB83_FUSOF
A0A0E0BI39_9ORYZ
R7EZE5_9BACI
A0A0A2LCU3_PENIT

C7N828_SLAHD
A0A0F0CDT0_9CLOT
A0A0D9VWL2_9ORYZ
T5AB61_OPHSC

A0A088EZ19_9SPHI
Q0JDX1_ORYSJ
J2P0J4_9SPHN
W9SGS7_9ROSA

A0A0E0BI38_9ORYZ
A0A067H2H7_CITSI
X0DC39_FUSOX
A0A0M2HIA8_9MICO

D7WDW1_9CORY
A0A0D3CIU1_BRAOL
A0A0A8JGP8_BACSX
H7F3I4_9LIST

U5CZX7_AMBTC
E4MZB2_KITSK
V4M6U7_EUTSA
W5NKI1_LEPOC

A0A022R6Z5_ERYGU
R6QMS7_9FIRM
R6A7P3_9CLOT
W6AFA9_9MOLU

A0A087HAQ7_ARAAL
A0A0B0MES2_GOSAR
R0G708_9BRAS
D2BE00_STRRD

A0A024QAI4_9BACI
A9WUZ2_RENSM
C7R1E8_JONDD
A0A067TU98_9AGAR

A0A0D3ACJ2_BRAOL
A0A078E4T6_BRANA
G2X781_VERDV
Q9LII1_ARATH

D6DG89_CLOSC
D4MS05_9FIRM
SSB_ONYPE
R4UJY5_9MOLU

Q2NU12_SODGM
A0A0J8CQH7_BETVU
A0A078F1K6_BRANA
R5LJ38_9MOLU

I3WJ92_BIFBI
Q0F348_9PROT
A0A078G1R5_BRANA
M4F913_BRARP

A0A022LIZ9_9MICO
A0A0M3DCH8_9MICO
A0A0D9VWL4_9ORYZ
A0A0F0LRE6_9MICO

A0A093YQ38_9PEZI
A0A093XU41_9PEZI
A0A094DK53_9PEZI
D4LHA6_9FIRM

K5X983_PHACS
B4FZD7_MAIZE
F5YIL0_TREPZ
E8T6W4_THEA1

S7QK86_GLOTA
Q49Z44_STAS1
B9S4S1_RICCO
A0A0D9VWL1_9ORYZ

H5U4M8_9ACTN
A0A067J8I3_JATCU
W6S2J0_9CLOT
Q0RPP0_FRAAA

L3ZIZ7_TERRK
W0GPN8_9MOLU
E6S6A4_INTC7
E4RTC4_LEAB4

J0WYQ3_9BIFI
V7CAJ9_PHAVU
M7SRB7_EUTLA
U5H6L2_USTV1

C8XGL8_NAKMY
A0LVD2_ACIC1
R5W4M2_9DELT
E1ZTB4_CHLVA

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The following references may be referred to within the specification by author and date and are incorporated herein by reference in their entireties at the location within the specification where they are referenced.

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	Number	Date	Country
	62315336	Mar 2016	US
	62291499	Feb 2016	US

Recombinase Genome Editing

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