Claims
- 1. A method for recovering a glycosidase or a peptidase from a culture broth comprising a culture medium and the cells which have produced the glycosidase or peptidase, wherein less than 80% of the peptidase or glycosidase in the culture medium is soluble in the culture medium and the remaining peptidase or glycosidase in the culture medium is present in the culture medium as either a precipitate or in crystalline form, comprising the sequence:a) adjusting the pH of the culture broth, which contains both the culture medium and the cells, to a pH value between about 9.5 and about 13, and subsequently, b) removing the cells from the culture broth to obtain the culture medium which contains the glycosidase or peptidase.
- 2. A method as defined in claim 1, wherein said remaining peptidase not in a soluble form at pH 7.5 is bound to solids in the culture broth.
- 3. A method as defined in claim 1, wherein the broth is adjusted to a pH between about 9.75 and about 13.
- 4. A method as defined in claim 1, wherein the broth is adjusted to a pH between about 10 to 13.
- 5. A method as defined in claim 1, wherein, in step (i), the culture broth is treated with at least one flocculation agent before, during, or after the pH adjustment.
- 6. A method as defined in claim 5, wherein the flocculation agent is a soluble Fe or Al compound.
- 7. A method as defined in claim 6, wherein the Al compound is selected from the group consisting of aluminum hydroxychloride, a polymer aluminum chlorohydrate, and NaAlO2.
- 8. A method as defined in claim 1, wherein the glycosidase or peptidase is a recombinantly derived variant.
- 9. A method as defined in claim 1, wherein the glycosidase is an amylase or a mannanase.
- 10. A method as defined in claim 1, wherein the peptidase is a protease.
- 11. A method as defined in claim 1, further comprising, prior to step (b), adjusting the pH to a lower pH.
Priority Claims (2)
Number |
Date |
Country |
Kind |
1999 00085 |
Jan 1999 |
DK |
|
1999 01491 |
Oct 1999 |
DK |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of U.S. Provisional application No. 60/118,757, filed Feb. 5, 1999, the contents of which are fully incorporated herein by reference.
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Date |
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5340926 |
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Aug 1994 |
|
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Provisional Applications (1)
|
Number |
Date |
Country |
|
60/118757 |
Feb 1999 |
US |