Claims
- 1. A process for isolating and purifying protein from polycarboxylic acid-protein precipitates obtained by mixing an aqueous cheese whey solution with about 0.1 to about 1.0 percent, (weight/volume basis) based on the total volume of the mixture, of a polycarboxylic acid selected from the class consisting of poly-acrylic acid, hydrolyzed ethylene-maleic anhydride copolymers and hydrolyzed methyl vinyl ether-maleic anhydride copolymers at a pH from about 3 to 4.5 and at a temperature below about 30.degree.C., which comprises:
- a. forming an aqueous solution of said precipitate having a pH between about 6 to about 9;
- b. adding to said solution a substance selected from the class consisting of calcium salts, magnesium salts, barium salts and aluminum salts to form a precipitate with the polycarboxylic acid present; and
- c. separating the purified protein solution from the resulting precipitate.
- 2. A process as recited in claim 1, further comprising:
- a. contacting the purified protein solution with a substance selected from the class consisting of calcium phosphate and magnesium phosphate to form a precipitate with residual polycarboxylic acid present; and
- b. separating the substantially polycarboxylic acid free protein solution from the resulting precipitate.
- 3. A process as recited in claim 2, in which the purified protein solution is contacted with the substance selected from the class consisting of calcium phosphate and magnesium phosphate at a pH between about 6 to about 9.
- 4. A process as recited in claim 2, in which the substance selected from the class consisting of calcium phosphate and magnesium phosphate is present in the solution at a concentration from about 0.1 to about 1.0 percent (weight/volume basis) based upon the total volume of the solution.
CROSS REFERENCE TO RELATED APPLICATION
This application is a continuation-in-part of copending U.S. patent application Ser. No. 353,895, filed Apr. 23, 1973, now U.S. Pat. No. 3,883,448.
US Referenced Citations (5)
Continuation in Parts (1)
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Number |
Date |
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353895 |
Apr 1973 |
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