Claims
- 1. A method of producing an adjuvant response or stimulating the immune response of a warm blooded animal comprising injecting into said warm blooded animal an effective amount of a composition comprising refined detoxified endotoxin obtained from microorganisms of the Family Enterobacteriaciae and having no detectable 2-keto-deoxyoctanoate, between about 350 and 475 n moles/mg of phosphorus and between about 1700 and 2000 n moles/mg of fatty acids, in combination with a pharmaceutically acceptable carrier.
- 2. The method of claim 1 wherein said effective amount is between about 5 and 500 .mu.g/kg.
- 3. The method of claim 2 wherein said effective amount is between about 10 and 100.mu.g/kg.
- 4. The method of claim 1 wherein said composition is in lyophilized form.
- 5. The method of claim 1 wherein said composition is an oil droplet emulsion.
- 6. The method of claim 1 wherein the immune response comprises the activation of B lymphocytes in said warm blooded animal.
- 7. The method of claim 6 wherein said effective amount is between about 5 and 500 micrograms per total animal weight.
- 8. The method of claim 7 wherein said effective amount is between about 10 and 100 micrograms per total animal weight.
- 9. The method of claim 6 wherein said composition is an oil droplet emulsion.
- 10. The method of claim 1 wherein the immune response comprises the production of lymphokines in said warm blooded animal.
- 11. The method of claim 10 wherein said effective amount is between about 5 and 500 micrograms per total animal weight.
- 12. The method of claim 11 wherein said effective amount is between about 10 and 100 micrograms per total animal weight.
- 13. The method of claim 10 wherein said composition is in lyophilized form.
- 14. The method of claim 10 wherein said composition is an oil droplet emulsion.
- 15. The method of claim 1 wherein the adjuvant response comprises stimulating the production of antibodies in said warm blooded animal.
- 16. The method of claim 15 wherein said effective amount is between about 5 and 500 micrograms per total animal weight.
- 17. The method of claim 16 wherein said effective amount is between about 10 and 100 micrograms per total animal weight.
- 18. The method of claim 15 wherein said composition is in lyophilized form.
- 19. The method of claim 15 wherein said composition is an oil droplet emulsion.
- 20. A method of producing an adjuvant response or stimulating the immune response of a warm blooded animal comprising injecting into said warm blooded animal an effective amount of a composition comprising refined detoxified endotoxin having no detectable 2-keto-deoxyoctanoate, between about 350 and 475 n moles/mg of phosphorus and between about 1700 and 2000 n moles/mg of fatty acids, in combination with a pharmaceutically acceptable carrier, said refined detoxified endotoxin having been prepared by the steps of:
- (a) hydrolyzing an endotoxin extract obtained from microorganisms of the Family Enterobacteriaciae with an inorganic or organic acid;
- (b) lyophilizing the hydrolyzed product to obtain crude lipid A;
- (c) treating crude lipid A with a first solvent capable of dissolving fatty acids and impurities without dissolving said crude lipid A;
- (d) dissolving the resulting insoluble product in a second solvent in which said crude lipid A is soluble; and
- (e) passing the resulting solution through a chromatographic column to obtain the desired refined detoxified endotoxin.
Parent Case Info
This application is a continuation of Ser. No. 526,967, now abandoned, which is a continuation-in-part of Ser. No. 382,404, filed May 26, 1982, now U.S. Pat. No. 4,436,727.
US Referenced Citations (6)
Continuations (1)
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Number |
Date |
Country |
Parent |
526967 |
Aug 1983 |
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Continuation in Parts (1)
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Number |
Date |
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Parent |
382404 |
May 1982 |
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