Claims
- 1. A molecular conjugate comprising: a release tag compound covalently bound to an analyte to be analytically determined, to an analyte analog, or to a primary or secondary specific binding partner thereof, said release tag compound comprising:
- a signal group S, a release group Re, and a reactivity group Rx, group S being covalently linked to group Re, and group Re being further covalently linked to group Rx to define structure S-Re-Rx for said compound;
- Rx further comprising a functional group capable of forming a covalent bond with another molecule;
- Re further comprising a functionality selected from the group consisting of .alpha.-hydroxyketos and .beta.-hydroxyketos; and
- S further comprising a halogenated electron-absorbing organic group; and
- groups S and Re being mutually selected such that upon cleavage of said release tag at release group Re, signal group S is released in a volatile form suitable for electron capture determination in the gas phase.
- 2. The molecular conjugate of claim 1 wherein Re is an .alpha.-hydroxyketo group.
- 3. The molecular conjugate of claim 1 wherein Re is a .beta.-hydroxyketo group.
- 4. The molecular conjugate of claim 1 wherein Re is an .alpha.,.beta.-dihydroxyketo group.
- 5. The molecular conjugate of claim 1 wherein S is a group capable of release as a ketone compound.
- 6. The molecular conjugate of claim 1 wherein S is a group capable of release as a polyfluorinated ketone compound.
- 7. The molecular conjugate of claim 1 wherein said analyte is a polynucleotide.
- 8. The molecular conjugate of claim 1 wherein said analyte is a protein.
- 9. The molecular conjugate of claim 1 wherein said analyte is an antibody.
- 10. The molecular conjugate of claim 1 wherein said analyte is an antigen.
- 11. The molecular conjugate of claim 1 wherein said analyte is a hapten.
- 12. The molecular conjugate of claim 1 wherein said analyte is avidin or an avidin analog.
- 13. The molecular conjugate of claim 12 wherein said analyte is streptavidin.
RELATED APPLICATIONS
This application is a division of application Ser. No. 08/053,608, filed Apr 22, 1993, U.S. Pat. No. 5,516,931 and is a continuation-in-part of application Ser. No. 06/710,318 filed Mar. 11, 1985, U.S. Pat. No. 5,360,819 which was a continuation-in-part of application Ser. No. 06/344,394 filed Feb. 1, 1982, now U.S. Pat. No. 4,709,016, the whole of which are hereby incorporated by reference herein.
GOVERNMENT SUPPORT
Part of this invention was made with government support. Therefore, the U.S. Government has certain rights in this invention.
US Referenced Citations (3)
Non-Patent Literature Citations (6)
Entry |
Buchet et al., "Le dosage par chromatographie en phase gaseuse des m etabolites urinaires du trichlor ethylene: l'acide trichloroac etique et le trichloro ethanol", Archives de Maladies Professionelles de M edecine due Travial et de S ecurit e Sociale (Paris), 35:395-402 (1974). |
Kawamatsu et al., Eur. J. Med. Chem.--Chim. Ther., 16(4) 355-362 [CA95(21): 187138c (1981)]. |
Landegren et al., "DNA Diagnostics--Molecular Techniques and Automation," Science 242:229-237 (1988). |
Rotman, "Sequencing the entire human genome," Industrial Chemist, pp. 18-21, Dec. 1987. |
Rudinger-Adler et al., "Arzneim.-Forsch.," 29(9) 1326-1331 [CA92(5): 41712b (1979)]. |
Senft, "Simple determination of trichloroacetic acid in urine using head space gas chromatography: a suitable method for monitoring exposure to trichloroethylene," Journal of Chromatography 337:126-130 (1985). |
Divisions (1)
|
Number |
Date |
Country |
Parent |
53608 |
Apr 1993 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
344394 |
Feb 1982 |
|