Claims
- 1. In an assay which includes contacting an analyte-containing sample with a labeled reagent, or with a conjugate made by covalently reacting a labeled reagent with standard said analyte, or with an analog of, ligand for, or specific binding partner of said analyte, incubating said contacted sample, and measuring the amount of label, the improvement which comprises:
- using as the labeled reagent a release tag compound, S-Re-Rx, wherein S is a signal group covalently bound to group Re, and Re is a cleavable release group which is covalently bound to group Rx which is a reactivity group;
- and S is further defined as comprising a halogenated electron-absorbing group which can be measured by electron capture detection;
- and Re is further defined as comprising a functionality selected from the group consisting of .alpha.-hydroxyketos and .beta.-hydroxyketos, and is capable of releasing the signal group in a volatile form suitable for measuring by electron capture detection;
- and Rx is further defined as comprising a functional group which is capable of forming a covalent bond with another molecule.
- 2. In an assay which includes contacting an analyte-containing sample with a labeled reagent, or with a conjugate made by covalently reacting a labeled reagent with standard said analyte, or with an analog of ligand for, or specific binding partner of said analyte, incubating said contacted sample, and measuring the amount of label, the improvement which comprises:
- using as the labeled reagent a release tag compound, S-Re-Rx, wherein S is a signal group covalently bound to group Re, and Re is a cleavable release group which is covalently bound to group Rx which is a reactivity group;
- and S is further defined as comprising a halogenated electron-absorbing group which can be measured by electron capture detection;
- and Re is further defined as comprising a functionality cleavable by heat and is capable of releasing the signal group in a volatile form suitable for measuring in the gas phase;
- and Rx is further defined as comprising a functional group which is capable of forming a covalent bond with another molecule;
- said improvement further comprising measuring the amount of label following thermal release of the signal group.
- 3. The assay of claim 2 wherein Re is a .beta.-hydroxyketo group.
- 4. The assay of claim 2 wherein Re is an .alpha.,.beta.-dihydroxyketo group.
- 5. The assay of claim 2 wherein said signal group is released as a ketone compound.
- 6. The assay of claim 2 wherein said signal group is released as a polyfluorinated ketone compound.
- 7. The assay of claim 1 wherein Re is an .alpha.,.beta.-dihydroxyketo group.
- 8. The assay of claim 1 wherein said signal group is released as a ketone compound.
- 9. The assay of claim 1 wherein said signal group is released as a polyfluorinated ketone compound.
RELATED APPLICATIONS
This application is a division of application Ser. No. 08/053,608, filed Apr. 22, 1993 now U.S. Pat. No. 5,516,931 which is application is a continuation-in-part of application Ser. No. 06/710,318 filed Mar. 11, 1985, now U.S. Pat. No. 5,366,819, which was a continuation-in-part of application Ser. No. 06/344,394 filed Feb. 1, 1982, now U.S. Pat. No. 4,709,016, the whole of which are hereby incorporated by reference herein.
GOVERNMENT SUPPORT
Part of this invention was made with government support. Therefore, the U.S. Government has certain rights in this invention.
US Referenced Citations (3)
Non-Patent Literature Citations (6)
Entry |
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Divisions (1)
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Number |
Date |
Country |
Parent |
53608 |
Apr 1993 |
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Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
710318 |
Mar 1985 |
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Parent |
344394 |
Feb 1982 |
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