Claims
- 1. A method for treating a fluid to reduce pathogens which may be present therein, the fluid containing one or more components selected from the group consisting of blood and blood components, the method comprising:
(a) removing substantially all adenine from the fluid; (b) mixing a pathogen reduction-effective, substantially non-toxic amount of an endogenous photosensitizer or endogenously-based derivative photosensitizer with the fluid; (c) exposing the fluid containing the photosensitizer to photoradiation of sufficient wavelength and energy to activate the photosensitizer, whereby the pathogens are reduced.
- 2. The method of claim 1, wherein the removing step comprises adding a sufficient volume of a diluting solution to the fluid so that the adenine in the fluid is diluted to an extent that formation of a photosensitizer-adenine complex is substantially prevented.
- 3. The method of claim 2, wherein the diluting solution is saline.
- 4. The method of claim 2, wherein the diluting solution is a buffer.
- 6. The method of claim 2, wherein the diluting solution is a commercially available cell storage solution which does not contain adenine.
- 7. The method of claim 2, wherein the diluting solution is an anticoagulant which does not contain adenine.
- 8. The method of claim 2, wherein the diluting solution is a cryopreservative solution which does not contain adenine.
- 9. The method of claim 1, wherein the removing step comprises washing the fluid.
- 10. The method of claim 9 wherein the removing step further comprises a plurality of washing steps.
- 11. The method of claim 1 wherein the fluid to be treated contains plasma and wherein the removing step comprises removing substantially all plasma which may be present in the fluid.
- 12. The method of claim 1 wherein the removing step comprises filtering out substantially all of the adenine present.
- 13. The method of claim 12 wherein the removing step further comprises filtering out substantially all adenine present using a filter having a filter media which selectively binds to adenine.
- 14. The method of claim 13 wherein the filtering step occurs outside of a container for containing fluid to be pathogen reduced.
- 15. The method of claim 13 wherein the filtering step occurs inside of a container for containing fluid to be pathogen reduced.
- 16. The method of claim 1 wherein the removing step comprises adding a substrate to the fluid wherein the substrate binds up substantially all adenine present in the fluid.
- 17. The method of claim 1 wherein the photosensitizer is 7,8-dimethyl-10-ribityl isoalloxazine.
- 18. The method of claim 1 wherein a quencher is added to the fluid after the removing step and prior to the exposing step.
- 19. The method of claim 18 wherein the quencher is selected from the group consisting of histidine, cysteine, tyrosine, tryptophan, ascorbate, N-acetyl-L-cysteine, propyl gallate, glutathione, mercaptopropionylglycine, dithiothreotol, nicotinamide, BHT, BHA, lysine, serine, methionine, glucose, mannitol, vitamin E, alpha-tocopherol acetate, trolox, glycerol, and mixtures thereof.
- 20. The method of claim 1 wherein the method substantially prevents damage to the blood or blood components contained in the fluid by preventing formation of a photosensitizer-adenine complex.
- 21. The method of claim 1 wherein the method substantially prevents an increase in oxidation reactions produced by photolysis of the photosensitizer.
- 22. The method of claim 1 wherein the mixing step further comprises placing the fluid in a container transparent to photoradiation, adding the photosensitizer to the fluid in the container and agitating the container.
- 23. The method of claim 1, wherein the adenine is substantially removed from the fluid prior to placing the fluid in a container for the exposing step.
- 24. The method of claim 1 wherein the adenine is substantially removed from the fluid after placing the fluid in a container for the exposing step.
- 25. The method of claim 1 wherein the fluid comprises whole blood.
- 27. The method of claim 1 wherein the fluid consists essentially of platelets.
- 28. The method of claim 1 wherein the fluid consists essentially of red blood cells.
- 29. A fluid for pathogen reduction that is substantially adenine-free comprising:
one or more components selected from the group consisting of blood and blood components; an endogenous photosensitizer or an endogenously-based derivative photosensitizer; and an adenine-free solution.
PRIORITY CLAIM
[0001] This application claims priority from U.S. Provisional No. 60/375,849 filed Apr. 24, 2002.
Provisional Applications (1)
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Number |
Date |
Country |
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60375849 |
Apr 2002 |
US |