REPLICATION FUNCTION OF SACCHAROMYCES CEREVESIAE

Information

• Research Project
• 3286410

• ApplicationId

  3286410

• Core Project Number

  R01GM034808

• Full Project Number

  5R01GM034808-04

• Serial Number

  34808

• FOA Number

• Sub Project Id

• Project Start Date

  9/1/1984 - 40 years ago

• Project End Date

  2/28/1989 - 35 years ago

• Program Officer Name

• Budget Start Date

  3/1/1987 - 37 years ago

• Budget End Date

  2/29/1988 - 36 years ago

• Fiscal Year

  1987

• Support Year

  4

• Suffix

• Award Notice Date

  2/18/1987 - 37 years ago

Organizations

• ZymoGenetics, Inc.

Nucleosome assembly will studied in vitro using extracts of HeLa cell nuclei as a source of somatic cell nucleosome assembly factors. The appropriate DNA, histone modification state, incubation conditions will be determined for study of in vitro assembly. The formation of pre-nucleosomal histone-histone complexes, histone-DNA complexes, and factor-DNA complexes will be examined in order to establish the assembly pathway. Chromatin assembled in vitro will be tested for its ability to be transcribed by injection into Xenopus oocytes. Incubation of chromatin extracts derived from cells actively expressing sequences to be tested will be performed before, during, and after assembly in order to study the formation of transcriptionally active chromatin.

IC Name

• Activity

  R01

• Administering IC

  GM

• Application Type

  5

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  862

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  CTY

• Study Section Name

  Molecular Cytology Study Section

• Organization Name

  ZYMOGENETICS, INC.

• Organization Department

• Organization DUNS

• Organization City

  SEATTLE

• Organization State

  WA

• Organization Country

  UNITED STATES

• Organization Zip Code

  98105

• Organization District

  UNITED STATES