Claims
- 1. A process for cell culture propagation of hepatitis A virus comprising the steps of:
- a) acutely infecting susceptible cells with strain HAS-15 hepatitis A viral particles at a multiplicity of infection of approximately 300 infectious viral particles per cell, said infection resulting in cell lysis and subsequent release of viral particles; and
- (b) isolating and purifying said released viral particles of step a).
- 2. The process of claim 1 wherein acute injection is maintained by addition of uninfected cells when 75% of the original cells have lysed.
- 3. The process of claim 1 wherein said susceptible cells of step a) are FRhK4 foetal rhesus monkey kidney cells.
- 4. The process of claims 1 or 3 wherein said hepatitis A particles have an antigen coded by a VP1 region nucleotide sequence according to FIG. 1.
Parent Case Info
This is a continuation of application Ser. No. 07/211,973, filed on Jun. 27, 1988, which was abandoned upon the filing hereof.
US Referenced Citations (11)
Non-Patent Literature Citations (1)
Entry |
Anderson et al. J. Cell. Biochem. vol. 10D p. 287 (1986). |
Continuations (1)
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Number |
Date |
Country |
Parent |
211973 |
Jun 1988 |
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