Patent Application
20120140215
1. Field of the Invention
The present invention relates to a retro-reflector microarray particularly suitable for biomedical optics and photonic detection. The retro-reflector microarray can reflect stimulated emission (fluorescence and Raman) whereby fluorescence and Raman signals of samples can be distantly scanned.
2. Description of the Related Art
In the field of biomedicine sensing, nucleic acid amplification is often used to duplicate and amplify a small amount of a nucleic acid sample to a detectable amount. The amplified nucleic acid product is further treated with probe hybridization so that nucleic acid probes with fluorescent dye can be linked to the target nucleic acid fragments. By being irradiated with the excitation light source having wavelengths matching to the fluorescence (and Raman signal), the target nucleic acid will emit specific optical signals (fluorescence and Raman signals). The signals are then detected and analyzed by the imaging and spectroscopy analyzer.
For the specific fluorescent dyes, the excitation light source with a specific range of wavelengths is required. When irradiated by light beams of proper wavelengths (e.g. ultraviolet), the fluorescence molecules will absorb the energy of the light and transit to a higher energy level. Then within a very short period of time (10−8-10−4 second), the electrons will return to the lower energy level and release energy in the form of fluorescence. If the transition to the higher states is virtual, the emitted signal is then a Raman one. The spontaneous fluorescence and Raman scattering are spatially isotropic (i.e., emitting in a solid angle of 4π) or non-coherent. In order to collect more non-coherent fluorescence and Raman signals, the optical elements (such as high numerical aperture optics) have to be positioned very close to the sample due to the small size of the lens. However, the numerical apertures of the optical elements may still be limited so that the strengths of the collected signals are insufficient and noises may be relatively high.
On the other hand, it is also known that excited fluorescence molecules can generate relatively coherent fluorescence signals through stimulated emission and the detection limit can reach 10−21 mol. The coherent fluorescent signals can then be distantly detected by optical elements with small numerical apertures. Effects of the corresponding optical components should then be improved.
The object of the present invention is to provide a retro-reflector shaped microarray. The retro-reflector microarray includes a plurality of micro retro-reflectors arranged on a common plane to form a microarray structure. The retro-reflectors can be arranged continuously or discreetly, regularly or irregularly, and as duplicated patterns or else. The retro-reflector shaped (or patterned) microarray can reflect coherent stimulated fluorescence and Raman emission induced by laser so that samples can be distantly scanned and the fluorescence and Raman signals can achieve higher strengths and lower noise-to-signal ratios.
Each of the retro-reflectors is a concave corner cube consisting of three mutually orthogonal reflective surfaces along the X-, Y- and Z-axes. Within each retro-reflector, v-shaped grooves constituted by two adjacent reflective surfaces along the X-, Y- and Z-axes form a main reflecting component to make the reflected light parallel to the incident light. The retro-reflector microarray can be an auxiliary element used in biomedical optical detection for distant scanning of fluorescence and Raman signals.
To further describe the present invention, the preferred embodiment is illustrated. It is necessary to note that elements are drawn for explaining the ratio, size, deformation or displacement but not proportional to the real elements. Furthermore, similar elements are designated identical numbers in the drawings.
The retro-reflector microarray of the present invention can be used to distantly scan samples and collect fluorescence and Raman signals generated by stimulated emission. The invention is further exemplified by detecting a nucleic acid sample coupled with fluorescent dye. Laser is provided as an excitation source and a stimulating source of fluorescence and Raman. When the sample is irradiated with excitation beams and stimulating beams, electrons thereof transit to a higher energy (virtual) level and then return with fluorescence (and Raman) emission. The fluorescence and Raman emission is highly coherent.
In the present invention, the retro-reflectors are arranged in a microarray way on a common plane to receive the incident beam (stimulated beam or stimulated coherent fluorescence and Raman) from each scanning point of the sample (50). The coherent fluorescence and Raman beams from all scanning points will reach to the detector. The positions and the angles that the reflected beams return to the scanner (40) are predictable. The detector can thus receive all the reflected beams at a fixed position. The detector can also be installed distance from the sample to collect all the reflected beams from each scanning point. In addition, such a remote scanning can promote strengths of the signals, lower noise-to-signal ratio and increase accuracy in interpreting the fluorescence and Raman signals.
While this invention has been particularly illustrated with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims. Any modification or change not departing from the main idea of the present invention is within the scope of the patent claimed.
| Number | Date | Country | Kind |
|---|---|---|---|
| 099142559 | Dec 2010 | TW | national |
